RchiOBHm_Chr6g0281711

Tetraketide alpha-pyrone reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008638
Physical Location & Seq
Reverse (-)
45034647 .. 45036792
2146 bp
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UTR
Exon/CDS
Intron
PRQ25267

Sequence Viewer

Length: 990 bp
ATGGATCAAAAACTTGAATCCGAAAGCAGAGTTTGTGTCACGGGAGCTTCTGGCTTTCTGGCATCTTGGCTCATTAAGCGACTTCTCATGTCAGGATATCATGTAACTGGAACTGTCAGAGATCCAGGAAACCAGAAGAAATTGGCACATTTGTGGAGGCTGGAAGGAGCTAAAGAAAGACTGAGATTGGTGAAGGCTGATTTAGTTGAAGAAGGTAGCTTTGATGATGCAATCTTCGGGTGCCATGGTGTCTTCCACTCTGCTTCTCCTGTACCCAAACTTTCATCTGATCCAAAGAAAGAAATCCTAGACCCCGCTATTGAAGGCACGTTGAATGTACTTCGTTCATGTAAGAAGAATCCATCTCTAAGGCGTGTGGTTCTCACCTCATCTTCTTCAGCTGTAAGGGTGAGAGATGGTGATGATTTTGACCCCAATATTCCATTGGATGAGTCATCTTGGAGCTCTGTGGAACTCTGCCAGAAACTGCAGATTTGGTACCCTTTATCAAAAATTTTAGCTGAGAGGGCAGCATCGGATTTCTGCAAAGCAAATGGGATCGATTTAGTAACCGTTCTACCCACATTCGTCGTTGGACCTAGTTTGCCACCTGATTTATGTTCTACTGCATCTGATATACTTGGCCTGCTCAAGGGTGAAACAGAGAGGTTCAAATTGCATGGAAGAATGGGATATGTTCACATCGATGATGTTGCACTTTGCCACATCCTAGTTTATGAGCACAAAAGTGCTCATAGTCGATACATTTGCAACTCGACAGTGCTTGACAACAATGAGTTAGCATCCTTGCTATCCACAAGATATCCTTCCTTACCTATCCCTGAAAGGTTTGAGCAACTGGAAAGGCCACACTATGATTTCAACACCTCAAAGTTGAGGGCTCTAGGATTTAAGTTCAAGACTGTCCGAGAGATGTTTGATGATTGCATTGCCTCTCTGGTGGAGCAAGGCCATCTTTCTCCATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase UFD1

Protein Analysis

329

Amino Acids

36.7

Weight (kDa)

6.6

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 11 - 251 5.5e-23 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 13 - 192 1.2e-12 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 13 - 203 2.9e-12 Male sterility protein
GDP_Man_Dehyd PF16363 13 - 132 1.7e-09 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 15 - 140 1.3e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 498
AccB1I GGYRCC 2 cut(s) 240, 498
AciI CCGC 1 cut(s) 315
AclWI GGATC 4 cut(s) 12, 116, 284, 566
AcsI RAATTY 1 cut(s) 513
AcuI CTGAAG 1 cut(s) 381
AfaI GTAC 3 cut(s) 273, 339, 500
AfiI CCNNNNNNNGG 1 cut(s) 652
AgsI TTSAA 7 cut(s) 17, 209, 323, 334, 673, 883, 919
AjnI CCWGG 1 cut(s) 124
AleI CACNNNNGTG 2 cut(s) 151, 747
AluBI AGCT 6 cut(s) 47, 170, 219, 401, 465, 521
AluI AGCT 6 cut(s) 47, 170, 219, 401, 465, 521
Alw21I GWGCWC 3 cut(s) 467, 744, 754
AlwI GGATC 4 cut(s) 12, 116, 284, 566
AlwNI CAGNNNCTG 1 cut(s) 487
AoxI GGCC 3 cut(s) 643, 866, 970
ApeKI GCWGC 1 cut(s) 530
ApoI RAATTY 1 cut(s) 513
Asp718I GGTACC 1 cut(s) 498
AspS9I GGNCC 1 cut(s) 596
AsuHPI GGTGA 5 cut(s) 202, 376, 421, 431, 668
AvaII GGWCC 1 cut(s) 596
BanI GGYRCC 2 cut(s) 240, 498
BanII GRGCYC 2 cut(s) 467, 904
BbsI GAAGAC 1 cut(s) 244
Bbv12I GWGCWC 3 cut(s) 467, 744, 754
BbvI GCAGC 1 cut(s) 542
BccI CCATC 3 cut(s) 370, 410, 981
BcgI CGANNNNNNTGC 4 cut(s) 695, 729, 750, 784
BciT130I CCWGG 1 cut(s) 126
BfaI CTAG 4 cut(s) 308, 600, 731, 905
BfmI CTRYAG 1 cut(s) 488
BisI GCNGC 1 cut(s) 531
BlsI GCNGC 1 cut(s) 532
Bme1390I CCNGG 1 cut(s) 126
Bme18I GGWCC 1 cut(s) 596
BmgT120I GGNCC 1 cut(s) 596
BmiI GGNNCC 2 cut(s) 242, 500
BmrFI CCNGG 1 cut(s) 126
BmsI GCATC 5 cut(s) 71, 217, 542, 638, 812
BpiI GAAGAC 1 cut(s) 244
BpuEI CTTGAG 1 cut(s) 635
Bsa29I ATCGAT 2 cut(s) 561, 705
BsaJI CCNNGG 1 cut(s) 244
Bsc4I CCNNNNNNNGG 1 cut(s) 652
Bse1I ACTGG 2 cut(s) 112, 864
Bse3DI GCAATG 1 cut(s) 948
BseBI CCWGG 1 cut(s) 126
BseCI ATCGAT 2 cut(s) 561, 705
BseDI CCNNGG 1 cut(s) 244
BseGI GGATG 3 cut(s) 454, 726, 803
BseLI CCNNNNNNNGG 1 cut(s) 652
BseMI GCAATG 1 cut(s) 948
BseMII CTCAG 2 cut(s) 173, 513
BseNI ACTGG 2 cut(s) 112, 864
BseXI GCAGC 1 cut(s) 542
BshFI GGCC 3 cut(s) 645, 868, 972
BshNI GGYRCC 2 cut(s) 240, 498
BshVI ATCGAT 2 cut(s) 561, 705
BsiHKAI GWGCWC 3 cut(s) 467, 744, 754
BslI CCNNNNNNNGG 1 cut(s) 652
BsnI GGCC 3 cut(s) 645, 868, 972
Bsp1286I GDGCHC 4 cut(s) 467, 744, 754, 904
Bsp143I GATC 4 cut(s) 4, 121, 289, 558
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 1 cut(s) 315
BspANI GGCC 3 cut(s) 645, 868, 972
BspCNI CTCAG 2 cut(s) 174, 514
BspDI ATCGAT 2 cut(s) 561, 705
BspLI GGNNCC 2 cut(s) 242, 500
BspMAI CTGCAG 1 cut(s) 492
BspPI GGATC 4 cut(s) 12, 116, 284, 566
BspT107I GGYRCC 2 cut(s) 240, 498
BsrDI GCAATG 1 cut(s) 948
BsrI ACTGG 2 cut(s) 112, 864
BssECI CCNNGG 1 cut(s) 244
BssMI GATC 4 cut(s) 4, 121, 289, 558
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 4 cut(s) 115, 574, 781, 925
BstC8I GCNNGC 1 cut(s) 647
BstDEI CTNAG 3 cut(s) 182, 368, 522
BstDSI CCRYGG 1 cut(s) 244
BstENI CCTNNNNNAGG 1 cut(s) 650
BstF5I GGATG 3 cut(s) 454, 726, 803
BstKTI GATC 4 cut(s) 7, 124, 292, 561
BstMBI GATC 4 cut(s) 4, 121, 289, 558
BstMWI GCNNNNNNNGC 2 cut(s) 76, 527
BstNI CCWGG 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 124
BstSFI CTRYAG 1 cut(s) 488
BstV1I GCAGC 1 cut(s) 542
BstV2I GAAGAC 1 cut(s) 244
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
Bsu15I ATCGAT 2 cut(s) 561, 705
BsuRI GGCC 3 cut(s) 645, 868, 972
BsuTUI ATCGAT 2 cut(s) 561, 705
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 3 cut(s) 454, 726, 803
BtsIMutI CAGTG 1 cut(s) 786
Cac8I GCNNGC 1 cut(s) 647
CaiI CAGNNNCTG 1 cut(s) 487
Cfr13I GGNCC 1 cut(s) 596
ClaI ATCGAT 2 cut(s) 561, 705
Csp6I GTAC 3 cut(s) 272, 338, 499
CviAII CATG 5 cut(s) 88, 101, 245, 348, 680
CviQI GTAC 3 cut(s) 272, 338, 499
DdeI CTNAG 3 cut(s) 182, 368, 522
DpnI GATC 4 cut(s) 6, 123, 291, 560
DpnII GATC 4 cut(s) 4, 121, 289, 558
Ecl136II GAGCTC 1 cut(s) 465
Eco130I CCWWGG 1 cut(s) 244
Eco24I GRGCYC 2 cut(s) 467, 904
Eco32I GATATC 2 cut(s) 98, 824
Eco47I GGWCC 1 cut(s) 596
Eco53kI GAGCTC 1 cut(s) 465
Eco57I CTGAAG 1 cut(s) 381
EcoICRI GAGCTC 1 cut(s) 465
EcoNI CCTNNNNNAGG 1 cut(s) 650
EcoRII CCWGG 1 cut(s) 124
EcoRV GATATC 2 cut(s) 98, 824
EcoT14I CCWWGG 1 cut(s) 244
EcoT38I GRGCYC 2 cut(s) 467, 904
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 5 cut(s) 91, 104, 248, 351, 683
FalI AAGNNNNNCTT 3 cut(s) 811, 843, 960
FatI CATG 5 cut(s) 87, 100, 244, 347, 679
FauI CCCGC 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 531
FokI GGATG 3 cut(s) 461, 713, 790
FriOI GRGCYC 2 cut(s) 467, 904
Fsp4HI GCNGC 1 cut(s) 531
FspBI CTAG 4 cut(s) 308, 600, 731, 905
GluI GCNGC 1 cut(s) 531
HaeIII GGCC 3 cut(s) 645, 868, 972
Hin1II CATG 5 cut(s) 91, 104, 248, 351, 683
HinfI GANTC 3 cut(s) 17, 358, 452
HphI GGTGA 5 cut(s) 202, 376, 421, 431, 668
Hpy166II GTNNAC 1 cut(s) 700
Hpy188I TCNGA 6 cut(s) 22, 119, 289, 538, 634, 929
Hpy188III TCNNGA 2 cut(s) 93, 919
Hpy8I GTNNAC 1 cut(s) 700
Hpy99I CGWCG 1 cut(s) 593
HpyAV CCTTC 5 cut(s) 158, 187, 206, 317, 837
HpyCH4III ACNGT 4 cut(s) 115, 574, 781, 925
HpyCH4IV ACGT 1 cut(s) 329
HpyCH4V TGCA 8 cut(s) 230, 490, 546, 629, 679, 716, 771, 948
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 527
HpyF3I CTNAG 3 cut(s) 182, 368, 522
HpySE526I ACGT 1 cut(s) 329
Hsp92II CATG 5 cut(s) 91, 104, 248, 351, 683
KpnI GGTACC 1 cut(s) 502
Kzo9I GATC 4 cut(s) 4, 121, 289, 558
LmnI GCTCC 4 cut(s) 44, 167, 462, 964
Lsp1109I GCAGC 1 cut(s) 542
LweI GCATC 5 cut(s) 71, 217, 542, 638, 812
MaeI CTAG 4 cut(s) 308, 600, 731, 905
MaeII ACGT 1 cut(s) 329
MaeIII GTNAC 3 cut(s) 37, 103, 568
MalI GATC 4 cut(s) 6, 123, 291, 560
MboI GATC 4 cut(s) 4, 121, 289, 558
MboII GAAGA 8 cut(s) 148, 221, 226, 244, 367, 384, 387, 696
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 4 cut(s) 467, 744, 754, 904
MluCI AATT 3 cut(s) 140, 513, 674
MlyI GAGTC 1 cut(s) 461
MmeI TCCRAC 1 cut(s) 574
MnlI CCTC 7 cut(s) 150, 397, 519, 660, 891, 898, 964
MseI TTAA 2 cut(s) 75, 912
MslI CAYNNNNRTG 3 cut(s) 151, 705, 747
MspA1I CMGCKG 1 cut(s) 401
MspR9I CCNGG 1 cut(s) 126
MvaI CCWGG 1 cut(s) 126
MwoI GCNNNNNNNGC 2 cut(s) 76, 527
NcoI CCATGG 1 cut(s) 244
NdeII GATC 4 cut(s) 4, 121, 289, 558
NlaIII CATG 5 cut(s) 91, 104, 248, 351, 683
NlaIV GGNNCC 2 cut(s) 242, 500
NmuCI GTSAC 1 cut(s) 37
OliI CACNNNNGTG 2 cut(s) 151, 747
PfeI GAWTC 2 cut(s) 17, 358
PfoI TCCNGGA 1 cut(s) 124
PkrI GCNGC 1 cut(s) 532
PleI GAGTC 1 cut(s) 460
PpsI GAGTC 1 cut(s) 460
Psp124BI GAGCTC 1 cut(s) 467
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 2 cut(s) 242, 500
PspPI GGNCC 1 cut(s) 596
PstI CTGCAG 1 cut(s) 492
PstNI CAGNNNCTG 1 cut(s) 487
PsuI RGATCY 1 cut(s) 121
PvuII CAGCTG 1 cut(s) 401
RsaI GTAC 3 cut(s) 273, 339, 500
RsaNI GTAC 3 cut(s) 272, 338, 499
RseI CAYNNNNRTG 3 cut(s) 151, 705, 747
SacI GAGCTC 1 cut(s) 467
SaqAI TTAA 2 cut(s) 75, 912
SatI GCNGC 1 cut(s) 531
Sau3AI GATC 4 cut(s) 4, 121, 289, 558
Sau96I GGNCC 1 cut(s) 596
SchI GAGTC 1 cut(s) 461
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 4 cut(s) 467, 744, 754, 904
SfaNI GCATC 5 cut(s) 71, 217, 542, 638, 812
SfcI CTRYAG 1 cut(s) 488
SinI GGWCC 1 cut(s) 596
SmiMI CAYNNNNRTG 3 cut(s) 151, 705, 747
SmlI CTYRAG 1 cut(s) 650
SmoI CTYRAG 1 cut(s) 650
Sse9I AATT 3 cut(s) 140, 513, 674
SsiI CCGC 1 cut(s) 315
SspI AATATT 1 cut(s) 439
SspMI CTAG 4 cut(s) 308, 600, 731, 905
SstI GAGCTC 1 cut(s) 467
StyD4I CCNGG 1 cut(s) 124
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 4 cut(s) 115, 574, 781, 925
TaiI ACGT 1 cut(s) 332
TaqI TCGA 4 cut(s) 561, 705, 760, 776
TasI AATT 3 cut(s) 140, 513, 674
TatI WGTACW 1 cut(s) 337
TfiI GAWTC 2 cut(s) 17, 358
Tru1I TTAA 2 cut(s) 75, 912
Tru9I TTAA 2 cut(s) 75, 912
TscAI CASTG 1 cut(s) 786
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 1 cut(s) 530
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 2 cut(s) 273, 336
TspRI CASTG 1 cut(s) 786
VpaK11BI GGWCC 1 cut(s) 596
XagI CCTNNNNNAGG 1 cut(s) 650
XapI RAATTY 1 cut(s) 513
XcmI CCANNNNNNNNNTGG 1 cut(s) 442
XspI CTAG 4 cut(s) 308, 600, 731, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.