Rroxscaffold_7G00186890

Tetraketide alpha-pyrone reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008638
Physical Location & Seq
Forward (+)
26235304 .. 26240681
5378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00186890.1

Sequence Viewer

Length: 1011 bp
ATGGGGTGCTTTTGTTTCAAGATGGATCAAAAACTTGAATCCGAAAGCAGAGTTTGCGTCACGGGAGCTTCTGGCTTTCTGGCATCTTGGCTTATTAAGCGACTTCTCATGTCAGGATATCATGTAACTGGAACTGTCAGAGATCCAGGAAACCAGAAGAAATTGGCACATTTGTGGAGGCTGGAAGGAGCTAAAGAAAGACTGAGATTGGTGAAGGCTGATTTAGTTGAAGAAGGTAGCTTTGATGATGCAATCTTCGGGTGCCATGGTGTCTTCCACTCTGCTTCTCCTGTACCCAAACTTTCATCTGATCCAAAGAAAGAAATCCTAGACCCGGCTATTGAAGGCACGTTGAATGTGCTTCGTTCATGTAAGAAGAATCCATCTCTAAGGCGTGTGGTTCTCACCTCATCTTCTTCAGCTGTAAGGGTGAGAGATGGTGATGATTTCGACCCCAATATTCCATTGGATGAGTCATCTTGGAGCTCTGTGGAACTCTGCGAGAAACTGCAGCTTTGGTACCCTTTATCAAAAATTTTAGCTGAGAGGGCAGCATTGGATTTCTGCAAAGCAAATGGGATCGATTTAGTAACCGTTCTACCCACATTCGTTGTTGGACCTAGTTTGCCACCTGATTTATGTTCTACTGCATCTGATATACTTGGCCTGCTCAAGGGTGAAACAGAGAGGTTCAATATGCATGGAAGAATGGGATATGTTCACATCGATGATGTTGCACTTTGTCACATCCAAGTTTATGAGCACAAAAGTGCTCATGGTCGATACATTTGCAACTCGACAGTGCTTGACAATAATGAGTTAACATCCTTGCTATCCACAAGATATCCTTCCTTACCTATCCCTGAAAGGTTTGAGCAATTGGAAAGGCCACACTATGATTTCAACACCTTGAAGTTGAGGGGTCTAGGATTTAAGTTCAAGACTGTCCAAGAGATGTTTGATGATTGCATTGCCTCTCTGGTGGAGCAAGGCCATCTTTCTCCATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase UFD1

Protein Analysis

336

Amino Acids

37.54

Weight (kDa)

6.27

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 18 - 258 9.6e-23 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 20 - 198 8.2e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 20 - 210 4.5e-12 Male sterility protein
GDP_Man_Dehyd PF16363 20 - 139 3.1e-09 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 22 - 147 1.4e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 519
AccB1I GGYRCC 2 cut(s) 261, 519
AclWI GGATC 4 cut(s) 33, 137, 305, 587
AcsI RAATTY 1 cut(s) 534
AcuI CTGAAG 1 cut(s) 402
AfaI GTAC 2 cut(s) 294, 521
AfiI CCNNNNNNNGG 2 cut(s) 334, 673
AgsI TTSAA 9 cut(s) 19, 38, 230, 344, 355, 694, 904, 913, 940
AjnI CCWGG 1 cut(s) 145
AleI CACNNNNGTG 2 cut(s) 172, 768
AluBI AGCT 7 cut(s) 68, 191, 240, 422, 486, 514, 542
AluI AGCT 7 cut(s) 68, 191, 240, 422, 486, 514, 542
Alw21I GWGCWC 3 cut(s) 488, 765, 775
AlwI GGATC 4 cut(s) 33, 137, 305, 587
AoxI GGCC 3 cut(s) 664, 887, 991
ApeKI GCWGC 2 cut(s) 511, 551
ApoI RAATTY 1 cut(s) 534
Asp718I GGTACC 1 cut(s) 519
AspS9I GGNCC 1 cut(s) 617
AsuC2I CCSGG 1 cut(s) 335
AsuHPI GGTGA 5 cut(s) 223, 397, 442, 452, 689
AvaII GGWCC 1 cut(s) 617
BanI GGYRCC 2 cut(s) 261, 519
BanII GRGCYC 1 cut(s) 488
BbsI GAAGAC 1 cut(s) 265
Bbv12I GWGCWC 3 cut(s) 488, 765, 775
BbvI GCAGC 2 cut(s) 523, 563
BccI CCATC 4 cut(s) 16, 391, 431, 1002
BcgI CGANNNNNNTGC 4 cut(s) 716, 750, 771, 805
BciT130I CCWGG 1 cut(s) 147
BcnI CCSGG 1 cut(s) 335
BfaI CTAG 4 cut(s) 329, 621, 926, 1009
BfmI CTRYAG 1 cut(s) 509
BisI GCNGC 2 cut(s) 512, 552
BlsI GCNGC 2 cut(s) 513, 553
Bme1390I CCNGG 2 cut(s) 147, 335
Bme18I GGWCC 1 cut(s) 617
BmgT120I GGNCC 1 cut(s) 617
BmiI GGNNCC 2 cut(s) 263, 521
BmrFI CCNGG 2 cut(s) 147, 335
BmsI GCATC 3 cut(s) 92, 238, 659
BpiI GAAGAC 1 cut(s) 265
BpuEI CTTGAG 1 cut(s) 656
BpuMI CCSGG 1 cut(s) 335
Bsa29I ATCGAT 2 cut(s) 582, 726
BsaJI CCNNGG 1 cut(s) 265
Bsc4I CCNNNNNNNGG 2 cut(s) 334, 673
Bse1I ACTGG 1 cut(s) 133
Bse3DI GCAATG 1 cut(s) 969
BseBI CCWGG 1 cut(s) 147
BseCI ATCGAT 2 cut(s) 582, 726
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 3 cut(s) 475, 747, 824
BseLI CCNNNNNNNGG 2 cut(s) 334, 673
BseMI GCAATG 1 cut(s) 969
BseMII CTCAG 2 cut(s) 194, 534
BseNI ACTGG 1 cut(s) 133
BseXI GCAGC 2 cut(s) 523, 563
BshFI GGCC 3 cut(s) 666, 889, 993
BshNI GGYRCC 2 cut(s) 261, 519
BshVI ATCGAT 2 cut(s) 582, 726
BsiHKAI GWGCWC 3 cut(s) 488, 765, 775
BsiSI CCGG 1 cut(s) 335
BslI CCNNNNNNNGG 2 cut(s) 334, 673
BsnI GGCC 3 cut(s) 666, 889, 993
Bsp1286I GDGCHC 3 cut(s) 488, 765, 775
Bsp143I GATC 4 cut(s) 25, 142, 310, 579
Bsp19I CCATGG 1 cut(s) 265
BspANI GGCC 3 cut(s) 666, 889, 993
BspCNI CTCAG 2 cut(s) 195, 535
BspDI ATCGAT 2 cut(s) 582, 726
BspLI GGNNCC 2 cut(s) 263, 521
BspMAI CTGCAG 1 cut(s) 513
BspPI GGATC 4 cut(s) 33, 137, 305, 587
BspT107I GGYRCC 2 cut(s) 261, 519
BsrDI GCAATG 1 cut(s) 969
BsrI ACTGG 1 cut(s) 133
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 4 cut(s) 25, 142, 310, 579
BssT1I CCWWGG 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 4 cut(s) 136, 595, 802, 946
BstAPI GCANNNNNTGC 1 cut(s) 54
BstC8I GCNNGC 1 cut(s) 668
BstDEI CTNAG 3 cut(s) 203, 389, 543
BstDSI CCRYGG 1 cut(s) 265
BstENI CCTNNNNNAGG 1 cut(s) 671
BstF5I GGATG 3 cut(s) 475, 747, 824
BstKTI GATC 4 cut(s) 28, 145, 313, 582
BstMBI GATC 4 cut(s) 25, 142, 310, 579
BstMWI GCNNNNNNNGC 3 cut(s) 54, 97, 548
BstNI CCWGG 1 cut(s) 147
BstSCI CCNGG 2 cut(s) 145, 333
BstSFI CTRYAG 1 cut(s) 509
BstV1I GCAGC 2 cut(s) 523, 563
BstV2I GAAGAC 1 cut(s) 265
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
Bsu15I ATCGAT 2 cut(s) 582, 726
BsuRI GGCC 3 cut(s) 666, 889, 993
BsuTUI ATCGAT 2 cut(s) 582, 726
BtgI CCRYGG 1 cut(s) 265
BtsCI GGATG 3 cut(s) 475, 747, 824
BtsIMutI CAGTG 1 cut(s) 807
Cac8I GCNNGC 1 cut(s) 668
Cfr13I GGNCC 1 cut(s) 617
ClaI ATCGAT 2 cut(s) 582, 726
CseI GACGC 1 cut(s) 46
Csp6I GTAC 2 cut(s) 293, 520
CspCI CAANNNNNGTGG 2 cut(s) 592, 627
CviAII CATG 6 cut(s) 109, 122, 266, 369, 701, 776
CviQI GTAC 2 cut(s) 293, 520
DdeI CTNAG 3 cut(s) 203, 389, 543
DpnI GATC 4 cut(s) 27, 144, 312, 581
DpnII GATC 4 cut(s) 25, 142, 310, 579
Ecl136II GAGCTC 1 cut(s) 486
Eco130I CCWWGG 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 488
Eco32I GATATC 2 cut(s) 119, 845
Eco47I GGWCC 1 cut(s) 617
Eco53kI GAGCTC 1 cut(s) 486
Eco57I CTGAAG 1 cut(s) 402
EcoICRI GAGCTC 1 cut(s) 486
EcoNI CCTNNNNNAGG 1 cut(s) 671
EcoRII CCWGG 1 cut(s) 145
EcoRV GATATC 2 cut(s) 119, 845
EcoT14I CCWWGG 1 cut(s) 265
EcoT22I ATGCAT 1 cut(s) 702
EcoT38I GRGCYC 1 cut(s) 488
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 6 cut(s) 112, 125, 269, 372, 704, 779
FalI AAGNNNNNCTT 3 cut(s) 832, 864, 981
FatI CATG 6 cut(s) 108, 121, 265, 368, 700, 775
Fnu4HI GCNGC 2 cut(s) 512, 552
FokI GGATG 3 cut(s) 482, 734, 811
FriOI GRGCYC 1 cut(s) 488
Fsp4HI GCNGC 2 cut(s) 512, 552
FspBI CTAG 4 cut(s) 329, 621, 926, 1009
GluI GCNGC 2 cut(s) 512, 552
HaeIII GGCC 3 cut(s) 666, 889, 993
HapII CCGG 1 cut(s) 335
HgaI GACGC 1 cut(s) 46
Hin1II CATG 6 cut(s) 112, 125, 269, 372, 704, 779
HincII GTYRAC 1 cut(s) 822
HindII GTYRAC 1 cut(s) 822
HinfI GANTC 3 cut(s) 38, 379, 473
HpaI GTTAAC 1 cut(s) 822
HpaII CCGG 1 cut(s) 335
HphI GGTGA 5 cut(s) 223, 397, 442, 452, 689
Hpy166II GTNNAC 2 cut(s) 721, 822
Hpy188I TCNGA 4 cut(s) 43, 140, 310, 655
Hpy188III TCNNGA 3 cut(s) 19, 114, 940
Hpy8I GTNNAC 2 cut(s) 721, 822
HpyAV CCTTC 5 cut(s) 179, 208, 227, 338, 858
HpyCH4III ACNGT 4 cut(s) 136, 595, 802, 946
HpyCH4IV ACGT 1 cut(s) 350
HpyCH4V TGCA 8 cut(s) 251, 511, 567, 650, 700, 737, 792, 969
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 97, 548
HpyF3I CTNAG 3 cut(s) 203, 389, 543
HpySE526I ACGT 1 cut(s) 350
Hsp92II CATG 6 cut(s) 112, 125, 269, 372, 704, 779
KpnI GGTACC 1 cut(s) 523
KspAI GTTAAC 1 cut(s) 822
Kzo9I GATC 4 cut(s) 25, 142, 310, 579
LmnI GCTCC 4 cut(s) 65, 188, 483, 985
Lsp1109I GCAGC 2 cut(s) 523, 563
LweI GCATC 3 cut(s) 92, 238, 659
MaeI CTAG 4 cut(s) 329, 621, 926, 1009
MaeII ACGT 1 cut(s) 350
MaeIII GTNAC 4 cut(s) 58, 124, 589, 743
MalI GATC 4 cut(s) 27, 144, 312, 581
MboI GATC 4 cut(s) 25, 142, 310, 579
MboII GAAGA 8 cut(s) 169, 242, 247, 265, 388, 405, 408, 717
MfeI CAATTG 1 cut(s) 878
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 3 cut(s) 488, 765, 775
MluCI AATT 3 cut(s) 161, 534, 878
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 1 cut(s) 595
MnlI CCTC 6 cut(s) 171, 418, 540, 681, 912, 985
Mph1103I ATGCAT 1 cut(s) 702
MseI TTAA 3 cut(s) 96, 821, 933
MslI CAYNNNNRTG 3 cut(s) 172, 726, 768
MspA1I CMGCKG 1 cut(s) 422
MspI CCGG 1 cut(s) 335
MspR9I CCNGG 2 cut(s) 147, 335
MunI CAATTG 1 cut(s) 878
MvaI CCWGG 1 cut(s) 147
MwoI GCNNNNNNNGC 3 cut(s) 54, 97, 548
NciI CCSGG 1 cut(s) 335
NcoI CCATGG 1 cut(s) 265
NdeII GATC 4 cut(s) 25, 142, 310, 579
NlaIII CATG 6 cut(s) 112, 125, 269, 372, 704, 779
NlaIV GGNNCC 2 cut(s) 263, 521
NmuCI GTSAC 2 cut(s) 58, 743
NsiI ATGCAT 1 cut(s) 702
OliI CACNNNNGTG 2 cut(s) 172, 768
PfeI GAWTC 2 cut(s) 38, 379
PfoI TCCNGGA 1 cut(s) 145
PkrI GCNGC 2 cut(s) 513, 553
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
Psp124BI GAGCTC 1 cut(s) 488
Psp6I CCWGG 1 cut(s) 145
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 2 cut(s) 263, 521
PspPI GGNCC 1 cut(s) 617
PstI CTGCAG 1 cut(s) 513
PsuI RGATCY 1 cut(s) 142
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 2 cut(s) 294, 521
RsaNI GTAC 2 cut(s) 293, 520
RseI CAYNNNNRTG 3 cut(s) 172, 726, 768
SacI GAGCTC 1 cut(s) 488
SaqAI TTAA 3 cut(s) 96, 821, 933
SatI GCNGC 2 cut(s) 512, 552
Sau3AI GATC 4 cut(s) 25, 142, 310, 579
Sau96I GGNCC 1 cut(s) 617
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 2 cut(s) 147, 335
SduI GDGCHC 3 cut(s) 488, 765, 775
SfaNI GCATC 3 cut(s) 92, 238, 659
SfcI CTRYAG 1 cut(s) 509
SinI GGWCC 1 cut(s) 617
SmiMI CAYNNNNRTG 3 cut(s) 172, 726, 768
SmlI CTYRAG 1 cut(s) 671
SmoI CTYRAG 1 cut(s) 671
Sse9I AATT 3 cut(s) 161, 534, 878
SspI AATATT 1 cut(s) 460
SspMI CTAG 4 cut(s) 329, 621, 926, 1009
SstI GAGCTC 1 cut(s) 488
StyD4I CCNGG 2 cut(s) 145, 333
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 4 cut(s) 136, 595, 802, 946
TaiI ACGT 1 cut(s) 353
TaqI TCGA 5 cut(s) 450, 582, 726, 781, 797
TasI AATT 3 cut(s) 161, 534, 878
TfiI GAWTC 2 cut(s) 38, 379
Tru1I TTAA 3 cut(s) 96, 821, 933
Tru9I TTAA 3 cut(s) 96, 821, 933
TscAI CASTG 1 cut(s) 807
TseFI GTSAC 2 cut(s) 58, 743
TseI GCWGC 2 cut(s) 511, 551
Tsp45I GTSAC 2 cut(s) 58, 743
TspDTI ATGAA 2 cut(s) 294, 357
TspRI CASTG 1 cut(s) 807
VpaK11BI GGWCC 1 cut(s) 617
XagI CCTNNNNNAGG 1 cut(s) 671
XapI RAATTY 1 cut(s) 534
XcmI CCANNNNNNNNNTGG 1 cut(s) 463
XspI CTAG 4 cut(s) 329, 621, 926, 1009
Zsp2I ATGCAT 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.