RLG00000012947

Tetraketide alpha-pyrone reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008638
Physical Location & Seq
Forward (+)
24213598 .. 24216382
2785 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012947

Sequence Viewer

Length: 1008 bp
ATGTTGGTGCTGGTCAGGATGGATCAAAAACTTGAATCTGAAAGCAGAGTTTGCGTCACGGGAGCTTCTGGCTCTCTGGCATCTTGGCTTATTAAGCGACTTCTCATGTCAGGATATCATGTAACTGGAACTGTCAGAGATCCAGGAAACAAGAAGAAATTGGCACATTTGTGGAGGCTGGAAGGAGCTAAAGAAAGACTGAGATTGGTGAAGGCTGATTTAGTTGAAGAAGGTAGCTTTGATGATGCAATCTTCGGGTGCCACGGTGTCTTCCACTCTGCTTCTCCTGTACCCAAACTTTCATCTGATCCAAAGAAAGAAATCCTAGACCCGGCTATCGAAGGCACGTTGAATGTGCTTCGTTCATGTAAGAAGAATCCATCTCTAAGGCGTGTGGTTCTCACCTCATCTTCTTCAGCTGTAAGGGTGAGAGATGGTGATGATTTTGACCCCAATATTCCATTGGATGAGTCATCTTGGAGCTCTGTGGAACTCTGTGAGAAACTGCAGATTTGGTACCCTTTATCAAAAATTTTAGCTGAGAGGGCAGCATTGGATTTCTGCAAAGCAAATGGGATCGATTTAGTAACCGTTCTACCCACATTCGTCGTTGGACCTAGTTTGCCACCTGATTTATGTTCTACTGCATCTGATATACTTGGCCTGCTCAAGGGTGAAACAGAGAGGTTCAAATTGCATGGAAGAATGGGATATATTCACATCGATGATGTTGCACTTTGCCACATCCTAGTTTATGAGCACAAAAGTGCTTATGGTCGATACATTTGCAACTCGACAGTGCTTGACAACAATGAGTTAGCATCCTTGCTATCCACAAGATATCCTTCCTTACCTATCCCTGAAAGGTTTGAGCAACTGGAAAGGCCACACTATGATTTCAACACCTCAAAGTTGAGGGGTCTTGGATTTAAGTTCAAGACTGTCCAAGAGATGTTTGATGATTGTATTGCCTCTTTGGTGGAGCAAGGCCATCTTTCTCCATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase UFD1

Protein Analysis

336

Amino Acids

37.34

Weight (kDa)

6.56

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 14 - 95 3.4e-06 NmrA-like family
Epimerase PF01370 17 - 256 7.8e-22 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 19 - 197 4.7e-11 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 19 - 209 3.3e-10 Male sterility protein
GDP_Man_Dehyd PF16363 19 - 138 6.2e-08 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 21 - 146 7.8e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 516
AccB1I GGYRCC 2 cut(s) 258, 516
AclWI GGATC 4 cut(s) 30, 134, 302, 584
AcsI RAATTY 1 cut(s) 531
AcuI CTGAAG 1 cut(s) 399
AfaI GTAC 2 cut(s) 291, 518
AfiI CCNNNNNNNGG 2 cut(s) 331, 670
AgsI TTSAA 6 cut(s) 35, 227, 352, 691, 901, 937
AjnI CCWGG 1 cut(s) 142
AleI CACNNNNGTG 2 cut(s) 169, 765
AluBI AGCT 6 cut(s) 65, 188, 237, 419, 483, 539
AluI AGCT 6 cut(s) 65, 188, 237, 419, 483, 539
Alw21I GWGCWC 2 cut(s) 485, 762
AlwI GGATC 4 cut(s) 30, 134, 302, 584
AoxI GGCC 3 cut(s) 661, 884, 988
ApeKI GCWGC 1 cut(s) 548
ApoI RAATTY 1 cut(s) 531
Asp718I GGTACC 1 cut(s) 516
AspS9I GGNCC 1 cut(s) 614
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 5 cut(s) 220, 394, 439, 449, 686
AvaII GGWCC 1 cut(s) 614
BanI GGYRCC 2 cut(s) 258, 516
BanII GRGCYC 1 cut(s) 485
BbsI GAAGAC 1 cut(s) 262
Bbv12I GWGCWC 2 cut(s) 485, 762
BbvI GCAGC 1 cut(s) 560
BccI CCATC 4 cut(s) 13, 388, 428, 999
BcgI CGANNNNNNTGC 4 cut(s) 713, 747, 768, 802
BciT130I CCWGG 1 cut(s) 144
BcnI CCSGG 1 cut(s) 332
BfaI CTAG 3 cut(s) 326, 618, 749
BfmI CTRYAG 1 cut(s) 506
BisI GCNGC 1 cut(s) 549
BlsI GCNGC 1 cut(s) 550
Bme1390I CCNGG 2 cut(s) 144, 332
Bme18I GGWCC 1 cut(s) 614
BmgT120I GGNCC 1 cut(s) 614
BmiI GGNNCC 2 cut(s) 260, 518
BmrFI CCNGG 2 cut(s) 144, 332
BmsI GCATC 4 cut(s) 89, 235, 656, 830
BpiI GAAGAC 1 cut(s) 262
BpuEI CTTGAG 1 cut(s) 653
BpuMI CCSGG 1 cut(s) 332
Bsa29I ATCGAT 2 cut(s) 579, 723
BsaJI CCNNGG 1 cut(s) 262
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 670
Bse1I ACTGG 2 cut(s) 130, 882
BseBI CCWGG 1 cut(s) 144
BseCI ATCGAT 2 cut(s) 579, 723
BseDI CCNNGG 1 cut(s) 262
BseGI GGATG 4 cut(s) 24, 472, 744, 821
BseLI CCNNNNNNNGG 2 cut(s) 331, 670
BseMII CTCAG 2 cut(s) 191, 531
BseNI ACTGG 2 cut(s) 130, 882
BseXI GCAGC 1 cut(s) 560
BshFI GGCC 3 cut(s) 663, 886, 990
BshNI GGYRCC 2 cut(s) 258, 516
BshVI ATCGAT 2 cut(s) 579, 723
BsiHKAI GWGCWC 2 cut(s) 485, 762
BsiSI CCGG 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 331, 670
BsnI GGCC 3 cut(s) 663, 886, 990
Bsp1286I GDGCHC 2 cut(s) 485, 762
Bsp143I GATC 4 cut(s) 22, 139, 307, 576
BspANI GGCC 3 cut(s) 663, 886, 990
BspCNI CTCAG 2 cut(s) 192, 532
BspDI ATCGAT 2 cut(s) 579, 723
BspLI GGNNCC 2 cut(s) 260, 518
BspMAI CTGCAG 1 cut(s) 510
BspPI GGATC 4 cut(s) 30, 134, 302, 584
BspT107I GGYRCC 2 cut(s) 258, 516
BsrI ACTGG 2 cut(s) 130, 882
BssECI CCNNGG 1 cut(s) 262
BssMI GATC 4 cut(s) 22, 139, 307, 576
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 5 cut(s) 133, 266, 592, 799, 943
BstAPI GCANNNNNTGC 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 665
BstDEI CTNAG 3 cut(s) 200, 386, 540
BstDSI CCRYGG 1 cut(s) 262
BstENI CCTNNNNNAGG 1 cut(s) 668
BstF5I GGATG 4 cut(s) 24, 472, 744, 821
BstKTI GATC 4 cut(s) 25, 142, 310, 579
BstMBI GATC 4 cut(s) 22, 139, 307, 576
BstMWI GCNNNNNNNGC 3 cut(s) 51, 94, 545
BstNI CCWGG 1 cut(s) 144
BstSCI CCNGG 2 cut(s) 142, 330
BstSFI CTRYAG 1 cut(s) 506
BstV1I GCAGC 1 cut(s) 560
BstV2I GAAGAC 1 cut(s) 262
BstX2I RGATCY 1 cut(s) 139
BstYI RGATCY 1 cut(s) 139
Bsu15I ATCGAT 2 cut(s) 579, 723
BsuRI GGCC 3 cut(s) 663, 886, 990
BsuTUI ATCGAT 2 cut(s) 579, 723
BtgI CCRYGG 1 cut(s) 262
BtsCI GGATG 4 cut(s) 24, 472, 744, 821
BtsIMutI CAGTG 1 cut(s) 804
Cac8I GCNNGC 1 cut(s) 665
Cfr13I GGNCC 1 cut(s) 614
ClaI ATCGAT 2 cut(s) 579, 723
CseI GACGC 1 cut(s) 43
Csp6I GTAC 2 cut(s) 290, 517
CviAII CATG 4 cut(s) 106, 119, 366, 698
CviQI GTAC 2 cut(s) 290, 517
DdeI CTNAG 3 cut(s) 200, 386, 540
DpnI GATC 4 cut(s) 24, 141, 309, 578
DpnII GATC 4 cut(s) 22, 139, 307, 576
Ecl136II GAGCTC 1 cut(s) 483
Eco24I GRGCYC 1 cut(s) 485
Eco32I GATATC 2 cut(s) 116, 842
Eco47I GGWCC 1 cut(s) 614
Eco53kI GAGCTC 1 cut(s) 483
Eco57I CTGAAG 1 cut(s) 399
EcoICRI GAGCTC 1 cut(s) 483
EcoNI CCTNNNNNAGG 1 cut(s) 668
EcoRII CCWGG 1 cut(s) 142
EcoRV GATATC 2 cut(s) 116, 842
EcoT38I GRGCYC 1 cut(s) 485
FaeI CATG 4 cut(s) 109, 122, 369, 701
FalI AAGNNNNNCTT 3 cut(s) 829, 861, 978
FatI CATG 4 cut(s) 105, 118, 365, 697
Fnu4HI GCNGC 1 cut(s) 549
FokI GGATG 4 cut(s) 31, 479, 731, 808
FriOI GRGCYC 1 cut(s) 485
Fsp4HI GCNGC 1 cut(s) 549
FspBI CTAG 3 cut(s) 326, 618, 749
GluI GCNGC 1 cut(s) 549
HaeIII GGCC 3 cut(s) 663, 886, 990
HapII CCGG 1 cut(s) 332
HgaI GACGC 1 cut(s) 43
Hin1II CATG 4 cut(s) 109, 122, 369, 701
HinfI GANTC 3 cut(s) 35, 376, 470
HpaII CCGG 1 cut(s) 332
HphI GGTGA 5 cut(s) 220, 394, 439, 449, 686
Hpy188I TCNGA 4 cut(s) 40, 137, 307, 652
Hpy188III TCNNGA 3 cut(s) 16, 111, 937
Hpy99I CGWCG 1 cut(s) 611
HpyAV CCTTC 5 cut(s) 176, 205, 224, 335, 855
HpyCH4III ACNGT 5 cut(s) 133, 266, 592, 799, 943
HpyCH4IV ACGT 1 cut(s) 347
HpyCH4V TGCA 7 cut(s) 248, 508, 564, 647, 697, 734, 789
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 94, 545
HpyF3I CTNAG 3 cut(s) 200, 386, 540
HpySE526I ACGT 1 cut(s) 347
Hsp92II CATG 4 cut(s) 109, 122, 369, 701
KpnI GGTACC 1 cut(s) 520
Kzo9I GATC 4 cut(s) 22, 139, 307, 576
LmnI GCTCC 4 cut(s) 62, 185, 480, 982
Lsp1109I GCAGC 1 cut(s) 560
LweI GCATC 4 cut(s) 89, 235, 656, 830
MaeI CTAG 3 cut(s) 326, 618, 749
MaeII ACGT 1 cut(s) 347
MaeIII GTNAC 3 cut(s) 55, 121, 586
MalI GATC 4 cut(s) 24, 141, 309, 578
MboI GATC 4 cut(s) 22, 139, 307, 576
MboII GAAGA 8 cut(s) 166, 239, 244, 262, 385, 402, 405, 714
MflI RGATCY 1 cut(s) 139
MhlI GDGCHC 2 cut(s) 485, 762
MluCI AATT 3 cut(s) 158, 531, 692
MlyI GAGTC 1 cut(s) 479
MmeI TCCRAC 1 cut(s) 592
MnlI CCTC 7 cut(s) 168, 415, 537, 678, 909, 916, 982
MseI TTAA 2 cut(s) 93, 930
MslI CAYNNNNRTG 3 cut(s) 169, 723, 765
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 2 cut(s) 144, 332
MvaI CCWGG 1 cut(s) 144
MwoI GCNNNNNNNGC 3 cut(s) 51, 94, 545
NciI CCSGG 1 cut(s) 332
NdeII GATC 4 cut(s) 22, 139, 307, 576
NlaIII CATG 4 cut(s) 109, 122, 369, 701
NlaIV GGNNCC 2 cut(s) 260, 518
NmuCI GTSAC 1 cut(s) 55
OliI CACNNNNGTG 2 cut(s) 169, 765
PfeI GAWTC 2 cut(s) 35, 376
PfoI TCCNGGA 1 cut(s) 142
PkrI GCNGC 1 cut(s) 550
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
Psp124BI GAGCTC 1 cut(s) 485
Psp6I CCWGG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 2 cut(s) 260, 518
PspPI GGNCC 1 cut(s) 614
PstI CTGCAG 1 cut(s) 510
PsuI RGATCY 1 cut(s) 139
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 2 cut(s) 291, 518
RsaNI GTAC 2 cut(s) 290, 517
RseI CAYNNNNRTG 3 cut(s) 169, 723, 765
SacI GAGCTC 1 cut(s) 485
SaqAI TTAA 2 cut(s) 93, 930
SatI GCNGC 1 cut(s) 549
Sau3AI GATC 4 cut(s) 22, 139, 307, 576
Sau96I GGNCC 1 cut(s) 614
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 2 cut(s) 144, 332
SduI GDGCHC 2 cut(s) 485, 762
SfaNI GCATC 4 cut(s) 89, 235, 656, 830
SfcI CTRYAG 1 cut(s) 506
SinI GGWCC 1 cut(s) 614
SmiMI CAYNNNNRTG 3 cut(s) 169, 723, 765
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
Sse9I AATT 3 cut(s) 158, 531, 692
SspI AATATT 1 cut(s) 457
SspMI CTAG 3 cut(s) 326, 618, 749
SstI GAGCTC 1 cut(s) 485
StyD4I CCNGG 2 cut(s) 142, 330
TaaI ACNGT 5 cut(s) 133, 266, 592, 799, 943
TaiI ACGT 1 cut(s) 350
TaqI TCGA 5 cut(s) 339, 579, 723, 778, 794
TasI AATT 3 cut(s) 158, 531, 692
TfiI GAWTC 2 cut(s) 35, 376
Tru1I TTAA 2 cut(s) 93, 930
Tru9I TTAA 2 cut(s) 93, 930
TscAI CASTG 1 cut(s) 804
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 1 cut(s) 548
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 2 cut(s) 291, 354
TspRI CASTG 1 cut(s) 804
VpaK11BI GGWCC 1 cut(s) 614
XagI CCTNNNNNAGG 1 cut(s) 668
XapI RAATTY 1 cut(s) 531
XcmI CCANNNNNNNNNTGG 1 cut(s) 460
XspI CTAG 3 cut(s) 326, 618, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.