MD10G1145300.v1.1

Tetraketide alpha-pyrone reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008638
Physical Location & Seq
Reverse (-)
23166770 .. 23168573
1804 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1145300.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGGATCGAAAACTCGAATCCAAAAGTAAAGTTTGTGTCACCGGGGCTTCTGGTTTTCTGGCATCCTGGCTCATTAAGCGGCTTCTCTTGTCCGGTTATCATGTAACAGGGACTGTAAGAGATCCAGGAAACGAGAAGAAATTGGCGCATTTGTGGAAGCTAGAAGGAGCTAAGGAAAGACTCAGATTGGTGAAGGCAGATTTGATGGAAGAAGGCAGCTTTGATGACGCAATCTTGGGGTGCCATGGTGTTTTCCACTCTGCTTCCCCTGTTTTGAAACCTTCATCTGATCCCAAGACAGAAATACTGGAACCGGCTGTTGAGGGCACCCTGAACGTGTTACGTTCGTGTAAGAAGAATCCGTCTCTAAGGCGCGTGGTCCTCACCTCATCTTCTTCAGCTGTAAGGGTGAGACCAGATGGGGATTTCTCCTCCAATGTACCGCTTGATGAGTTGTCTTGGAGCTCTGTCGAACTCTGCGAGACGCTTCAGATATGGTACCCTTTGTCGAAAATTTTAGCTGAGAAAGCAGCATGGGATTTCTGCAAAGAAAATGGGATTGATTTGGTAACCATCCTACCCTCTTTCGTGATCGGGCCTAGTTTGCCCCCTGATTTGTGTTCTACTGCATCAGATGTTCTTGGCCTGCTTAAAGGTACTACTACTGTTGATTTATGTCCTTGCATCATAAGCGCTTCGTTTAATCATGATCATAAGCGCGTTAAATGGTTATTTGTAGGAGAAACAGAGAAATTCAAATGGCATGGAAGGATGGGATATGTTCACATTGATGATGTTGCACTCTGTCACATCTTAGTATATGAGCATGAAAGTGCTCATGGTCGATATCTTTGCAGCTCAACAGTTTTGGACAACAACGAGTTGGCTTCCTTATTATCTGCAAGATATCCATCCTTACCTATCCCCGCAAGGTTTGAGCAACTAGGGAGGCCACATTATGAGATGAACACCTCAAAGATGGAGAGTCTAGGGTTCAAGTTTAAGAGTATCCAAGAGATGTTTGATGATTGCATTGCTTCTTTGGTGGAACAAGGCCATCTTCCTGCACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase UFD1

Protein Analysis

358

Amino Acids

39.61

Weight (kDa)

6.36

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 8 - 88 3.2e-06 NmrA-like family
Epimerase PF01370 11 - 276 4.6e-20 NAD dependent epimerase/dehydratase family
NAD_binding_4 PF07993 13 - 203 1.8e-13 Male sterility protein
3Beta_HSD PF01073 13 - 179 1.9e-11 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 13 - 132 4e-09 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 15 - 138 1.2e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 498
AccB1I GGYRCC 3 cut(s) 240, 326, 498
AccII CGCG 2 cut(s) 375, 720
AciI CCGC 3 cut(s) 79, 443, 927
AclWI GGATC 3 cut(s) 12, 116, 284
AcsI RAATTY 2 cut(s) 513, 752
AcuI CTGAAG 2 cut(s) 381, 473
AfaI GTAC 3 cut(s) 441, 500, 658
AfeI AGCGCT 1 cut(s) 694
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 3 cut(s) 277, 757, 997
AjnI CCWGG 2 cut(s) 65, 124
AluBI AGCT 7 cut(s) 160, 170, 219, 401, 465, 521, 858
AluI AGCT 7 cut(s) 160, 170, 219, 401, 465, 521, 858
Alw21I GWGCWC 2 cut(s) 467, 838
Alw26I GTCTC 3 cut(s) 369, 406, 476
AlwI GGATC 3 cut(s) 12, 116, 284
AlwNI CAGNNNCTG 1 cut(s) 113
Aor51HI AGCGCT 1 cut(s) 694
AoxI GGCC 4 cut(s) 596, 643, 950, 1054
ApeKI GCWGC 3 cut(s) 216, 530, 855
ApoI RAATTY 2 cut(s) 513, 752
Asp718I GGTACC 1 cut(s) 498
AspLEI GCGC 4 cut(s) 148, 375, 695, 720
AspS9I GGNCC 2 cut(s) 379, 596
AsuC2I CCSGG 1 cut(s) 43
AsuHPI GGTGA 4 cut(s) 31, 202, 376, 421
AvaII GGWCC 1 cut(s) 379
BaeGI GKGCMC 1 cut(s) 329
BanI GGYRCC 3 cut(s) 240, 326, 498
BanII GRGCYC 1 cut(s) 467
Bbv12I GWGCWC 2 cut(s) 467, 838
BbvI GCAGC 3 cut(s) 228, 542, 867
BccI CCATC 7 cut(s) 199, 413, 581, 766, 919, 973, 1065
BcgI CGANNNNNNTGC 2 cut(s) 834, 868
BciT130I CCWGG 2 cut(s) 67, 126
BciVI GTATCC 1 cut(s) 1019
BclI TGATCA 1 cut(s) 709
BcnI CCSGG 1 cut(s) 43
BcoDI GTCTC 3 cut(s) 369, 406, 476
BfaI CTAG 5 cut(s) 161, 600, 944, 989, 1072
BfoI RGCGCY 1 cut(s) 696
BfuI GTATCC 1 cut(s) 1019
BisI GCNGC 4 cut(s) 80, 217, 531, 856
BlsI GCNGC 4 cut(s) 81, 218, 532, 857
Bme1390I CCNGG 3 cut(s) 43, 67, 126
Bme18I GGWCC 1 cut(s) 379
BmgT120I GGNCC 2 cut(s) 379, 596
BmiI GGNNCC 4 cut(s) 242, 312, 328, 500
BmrFI CCNGG 3 cut(s) 43, 67, 126
BmsI GCATC 3 cut(s) 71, 638, 693
Bpu10I CCTNAGC 1 cut(s) 171
BpuMI CCSGG 1 cut(s) 43
BsaBI GATNNNNATC 1 cut(s) 910
BsaI GGTCTC 1 cut(s) 406
BsaJI CCNNGG 2 cut(s) 42, 244
BsaWI WCCGGW 1 cut(s) 92
Bse118I RCCGGY 1 cut(s) 313
Bse1I ACTGG 1 cut(s) 312
Bse3DI GCAATG 1 cut(s) 1032
Bse8I GATNNNNATC 1 cut(s) 910
BseBI CCWGG 2 cut(s) 67, 126
BseDI CCNNGG 2 cut(s) 42, 244
BseGI GGATG 4 cut(s) 62, 573, 777, 911
BseJI GATNNNNATC 1 cut(s) 910
BseMI GCAATG 1 cut(s) 1032
BseMII CTCAG 2 cut(s) 196, 513
BseNI ACTGG 1 cut(s) 312
BseRI GAGGAG 1 cut(s) 421
BseSI GKGCMC 1 cut(s) 329
BseXI GCAGC 3 cut(s) 228, 542, 867
BsgI GTGCAG 1 cut(s) 1050
Bsh1236I CGCG 2 cut(s) 375, 720
BshFI GGCC 4 cut(s) 598, 645, 952, 1056
BshNI GGYRCC 3 cut(s) 240, 326, 498
BsiHKAI GWGCWC 2 cut(s) 467, 838
BsiSI CCGG 3 cut(s) 42, 93, 314
BslFI GGGAC 1 cut(s) 124
BsmAI GTCTC 3 cut(s) 369, 406, 476
BsmBI CGTCTC 2 cut(s) 369, 476
BsmFI GGGAC 1 cut(s) 124
BsnI GGCC 4 cut(s) 598, 645, 952, 1056
Bso31I GGTCTC 1 cut(s) 406
Bsp1286I GDGCHC 3 cut(s) 329, 467, 838
Bsp143I GATC 5 cut(s) 4, 121, 289, 591, 709
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 3 cut(s) 79, 443, 927
BspANI GGCC 4 cut(s) 598, 645, 952, 1056
BspCNI CTCAG 2 cut(s) 195, 514
BspFNI CGCG 2 cut(s) 375, 720
BspHI TCATGA 1 cut(s) 706
BspLI GGNNCC 4 cut(s) 242, 312, 328, 500
BspPI GGATC 3 cut(s) 12, 116, 284
BspT107I GGYRCC 3 cut(s) 240, 326, 498
BspTNI GGTCTC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 1032
BsrFI RCCGGY 1 cut(s) 313
BsrI ACTGG 1 cut(s) 312
BssAI RCCGGY 1 cut(s) 313
BssECI CCNNGG 2 cut(s) 42, 244
BssMI GATC 5 cut(s) 4, 121, 289, 591, 709
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 2 cut(s) 67, 126
Bst4CI ACNGT 3 cut(s) 115, 667, 865
BstC8I GCNNGC 1 cut(s) 647
BstDEI CTNAG 5 cut(s) 171, 182, 368, 522, 814
BstDSI CCRYGG 1 cut(s) 244
BstEII GGTNACC 1 cut(s) 568
BstF5I GGATG 4 cut(s) 62, 573, 777, 911
BstFNI CGCG 2 cut(s) 375, 720
BstH2I RGCGCY 1 cut(s) 696
BstHHI GCGC 4 cut(s) 148, 375, 695, 720
BstKTI GATC 5 cut(s) 7, 124, 292, 594, 712
BstMAI GTCTC 3 cut(s) 369, 406, 476
BstMBI GATC 5 cut(s) 4, 121, 289, 591, 709
BstMWI GCNNNNNNNGC 4 cut(s) 76, 527, 604, 690
BstNI CCWGG 2 cut(s) 67, 126
BstPI GGTNACC 1 cut(s) 568
BstSCI CCNGG 3 cut(s) 41, 65, 124
BstSLI GKGCMC 1 cut(s) 329
BstUI CGCG 2 cut(s) 375, 720
BstV1I GCAGC 3 cut(s) 228, 542, 867
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BsuI GTATCC 1 cut(s) 1019
BsuRI GGCC 4 cut(s) 598, 645, 952, 1056
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 4 cut(s) 62, 573, 777, 911
Cac8I GCNNGC 1 cut(s) 647
CaiI CAGNNNCTG 1 cut(s) 113
CciI TCATGA 1 cut(s) 706
CfoI GCGC 4 cut(s) 148, 375, 695, 720
Cfr10I RCCGGY 1 cut(s) 313
Cfr13I GGNCC 2 cut(s) 379, 596
CseI GACGC 2 cut(s) 236, 493
Csp6I GTAC 3 cut(s) 440, 499, 657
CviAII CATG 7 cut(s) 101, 245, 534, 707, 764, 827, 839
CviQI GTAC 3 cut(s) 440, 499, 657
DdeI CTNAG 5 cut(s) 171, 182, 368, 522, 814
DpnI GATC 5 cut(s) 6, 123, 291, 593, 711
DpnII GATC 5 cut(s) 4, 121, 289, 591, 709
Ecl136II GAGCTC 1 cut(s) 465
Eco130I CCWWGG 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 467
Eco31I GGTCTC 1 cut(s) 406
Eco32I GATATC 2 cut(s) 848, 908
Eco47I GGWCC 1 cut(s) 379
Eco47III AGCGCT 1 cut(s) 694
Eco53kI GAGCTC 1 cut(s) 465
Eco57I CTGAAG 2 cut(s) 381, 473
Eco91I GGTNACC 1 cut(s) 568
EcoICRI GAGCTC 1 cut(s) 465
EcoO65I GGTNACC 1 cut(s) 568
EcoRII CCWGG 2 cut(s) 65, 124
EcoRV GATATC 2 cut(s) 848, 908
EcoT14I CCWWGG 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 467
ErhI CCWWGG 1 cut(s) 244
Esp3I CGTCTC 2 cut(s) 369, 476
FaeI CATG 7 cut(s) 104, 248, 537, 710, 767, 830, 842
FalI AAGNNNNNCTT 1 cut(s) 1044
FaqI GGGAC 1 cut(s) 124
FatI CATG 7 cut(s) 100, 244, 533, 706, 763, 826, 838
FauI CCCGC 1 cut(s) 934
FbaI TGATCA 1 cut(s) 709
Fnu4HI GCNGC 4 cut(s) 80, 217, 531, 856
FokI GGATG 4 cut(s) 49, 560, 784, 898
FriOI GRGCYC 1 cut(s) 467
Fsp4HI GCNGC 4 cut(s) 80, 217, 531, 856
FspBI CTAG 5 cut(s) 161, 600, 944, 989, 1072
GlaI GCGC 4 cut(s) 147, 374, 694, 719
GluI GCNGC 4 cut(s) 80, 217, 531, 856
HaeII RGCGCY 1 cut(s) 696
HaeIII GGCC 4 cut(s) 598, 645, 952, 1056
HapII CCGG 3 cut(s) 42, 93, 314
HgaI GACGC 2 cut(s) 236, 493
HhaI GCGC 4 cut(s) 148, 375, 695, 720
Hin1II CATG 7 cut(s) 104, 248, 537, 710, 767, 830, 842
Hin6I GCGC 4 cut(s) 146, 373, 693, 718
HinP1I GCGC 4 cut(s) 146, 373, 693, 718
HinfI GANTC 4 cut(s) 17, 180, 358, 985
HpaII CCGG 3 cut(s) 42, 93, 314
HphI GGTGA 4 cut(s) 31, 202, 376, 421
Hpy166II GTNNAC 1 cut(s) 784
Hpy188I TCNGA 4 cut(s) 185, 289, 492, 634
Hpy188III TCNNGA 2 cut(s) 589, 707
Hpy8I GTNNAC 1 cut(s) 784
HpyAV CCTTC 5 cut(s) 158, 187, 206, 291, 762
HpyCH4III ACNGT 3 cut(s) 115, 667, 865
HpyCH4IV ACGT 2 cut(s) 336, 343
HpyCH4V TGCA 8 cut(s) 546, 629, 684, 800, 855, 902, 1032, 1067
HpyF10VI GCNNNNNNNGC 4 cut(s) 76, 527, 604, 690
HpyF3I CTNAG 5 cut(s) 171, 182, 368, 522, 814
HpySE526I ACGT 2 cut(s) 336, 343
Hsp92II CATG 7 cut(s) 104, 248, 537, 710, 767, 830, 842
HspAI GCGC 4 cut(s) 146, 373, 693, 718
KpnI GGTACC 1 cut(s) 502
Ksp22I TGATCA 1 cut(s) 709
Kzo9I GATC 5 cut(s) 4, 121, 289, 591, 709
LmnI GCTCC 2 cut(s) 167, 462
Lsp1109I GCAGC 3 cut(s) 228, 542, 867
LweI GCATC 3 cut(s) 71, 638, 693
MaeI CTAG 5 cut(s) 161, 600, 944, 989, 1072
MaeII ACGT 2 cut(s) 336, 343
MaeIII GTNAC 5 cut(s) 37, 103, 339, 568, 806
MalI GATC 5 cut(s) 6, 123, 291, 593, 711
MboI GATC 5 cut(s) 4, 121, 289, 591, 709
MboII GAAGA 6 cut(s) 148, 221, 367, 384, 387, 1052
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 3 cut(s) 329, 467, 838
MluCI AATT 3 cut(s) 140, 513, 752
MlyI GAGTC 2 cut(s) 174, 994
MnlI CCTC 7 cut(s) 316, 392, 397, 442, 592, 942, 982
MseI TTAA 5 cut(s) 75, 651, 702, 723, 1002
MslI CAYNNNNRTG 2 cut(s) 789, 831
MspA1I CMGCKG 1 cut(s) 401
MspI CCGG 3 cut(s) 42, 93, 314
MspR9I CCNGG 3 cut(s) 43, 67, 126
MvaI CCWGG 2 cut(s) 67, 126
MvnI CGCG 2 cut(s) 375, 720
MwoI GCNNNNNNNGC 4 cut(s) 76, 527, 604, 690
NciI CCSGG 1 cut(s) 43
NcoI CCATGG 1 cut(s) 244
NdeII GATC 5 cut(s) 4, 121, 289, 591, 709
NlaIII CATG 7 cut(s) 104, 248, 537, 710, 767, 830, 842
NlaIV GGNNCC 4 cut(s) 242, 312, 328, 500
NmuCI GTSAC 2 cut(s) 37, 806
PagI TCATGA 1 cut(s) 706
PcsI WCGNNNNNNNCGW 1 cut(s) 477
PfeI GAWTC 2 cut(s) 17, 358
PfoI TCCNGGA 1 cut(s) 124
PkrI GCNGC 4 cut(s) 81, 218, 532, 857
PleI GAGTC 2 cut(s) 174, 993
PpsI GAGTC 2 cut(s) 174, 993
Psp124BI GAGCTC 1 cut(s) 467
Psp6I CCWGG 2 cut(s) 65, 124
PspEI GGTNACC 1 cut(s) 568
PspGI CCWGG 2 cut(s) 65, 124
PspN4I GGNNCC 4 cut(s) 242, 312, 328, 500
PspPI GGNCC 2 cut(s) 379, 596
PstNI CAGNNNCTG 1 cut(s) 113
PsuI RGATCY 1 cut(s) 121
PvuII CAGCTG 1 cut(s) 401
RsaI GTAC 3 cut(s) 441, 500, 658
RsaNI GTAC 3 cut(s) 440, 499, 657
RseI CAYNNNNRTG 2 cut(s) 789, 831
SacI GAGCTC 1 cut(s) 467
SaqAI TTAA 5 cut(s) 75, 651, 702, 723, 1002
SatI GCNGC 4 cut(s) 80, 217, 531, 856
Sau3AI GATC 5 cut(s) 4, 121, 289, 591, 709
Sau96I GGNCC 2 cut(s) 379, 596
SchI GAGTC 2 cut(s) 174, 994
ScrFI CCNGG 3 cut(s) 43, 67, 126
SduI GDGCHC 3 cut(s) 329, 467, 838
SfaNI GCATC 3 cut(s) 71, 638, 693
SinI GGWCC 1 cut(s) 379
SmiMI CAYNNNNRTG 2 cut(s) 789, 831
Sse9I AATT 3 cut(s) 140, 513, 752
SsiI CCGC 3 cut(s) 79, 443, 927
SspMI CTAG 5 cut(s) 161, 600, 944, 989, 1072
SstI GAGCTC 1 cut(s) 467
StyD4I CCNGG 3 cut(s) 41, 65, 124
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 3 cut(s) 115, 667, 865
TaiI ACGT 2 cut(s) 339, 346
TaqI TCGA 5 cut(s) 7, 15, 471, 509, 844
TasI AATT 3 cut(s) 140, 513, 752
TauI GCSGC 1 cut(s) 82
TfiI GAWTC 2 cut(s) 17, 358
Tru1I TTAA 5 cut(s) 75, 651, 702, 723, 1002
Tru9I TTAA 5 cut(s) 75, 651, 702, 723, 1002
TseFI GTSAC 2 cut(s) 37, 806
TseI GCWGC 3 cut(s) 216, 530, 855
Tsp45I GTSAC 2 cut(s) 37, 806
TspDTI ATGAA 3 cut(s) 273, 843, 980
TspGWI ACGGA 1 cut(s) 351
VpaK11BI GGWCC 1 cut(s) 379
XapI RAATTY 2 cut(s) 513, 752
XspI CTAG 5 cut(s) 161, 600, 944, 989, 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.