MD10G1190700.v1.1

Iron-sulfur protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007258
Physical Location & Seq
Forward (+)
28771234 .. 28778610
7377 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1190700.v1.1.491

Sequence Viewer

Length: 870 bp
ATGAAGGGGCTGCTCAAATCCTTCACTAGGGTTGGAGGAGGTGTTAGGGGTTATAGCGCGGCGAGGAAGGACCTTCGGATTGAGGGGGTTAAGCATGCCATAGCTGTTGCTTCTGGTAAAGGTGGTGTCGGCAAGTCCACCACTGCAGTTAATCTGGCTGTTGCACTTGCTAACAAGTGCCAAATGAAGGTTGGCTTGCTTGATGCTGATATATACGGACCAAATGTTCCTATAATGATGAAGCTTGATAGAAAGCCAGAAGTGACTGAAGATAAAAAAATGATTCCAATCGAGAGCTATGGAGTCAAGTGTATGTCAATGGGACTTCTTGTGGATAAGGATGCTCCGCTTGTTTGGAGAGGTCCCATGGTATCTAGCGCTCTTGAAAAAATGACTAGGGGCGCTGACTGGGGAAATCTTGATATTCTTGTGGTAGATATGCCTCCTGGCACTGGTGATGCTCATATAACTGTATCCCAAAGGCTGCAATTATCAGGTGCATTGATTGTTTCAACGCCTCAAGATGTAGCATTAATGGATGCCCGTAGAGGAATTAACATGTTCTCTAAAGTTGAAGTTCCCATTTTGGGAATTGTAGAGAACATGAGCTATTTTAAGTGTCCGCACTGTGCCGAATGTTCCTTCATTTTCGGGAAAGGAGGATCTCGTAAGACAGCAACTGAGATGGATTTGGAATTTGTTGGCGAGATACCGTTAGAAATGGGGATCAGAGAAGGCTCCGACAATGGTGTCCCAATAGTAATATCAGCTCCTGATTCTGATGTCTCGAAAGCATATGTGGACGTGGCTCGGAAAGTTGTAGAAAGATTGTCGAAGGAAGAACAATCCCGTCCACATATATGCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ParA

Protein Analysis

290

Amino Acids

31.09

Weight (kDa)

8.45

Isoelectric Point (pI)

34.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ParA PF10609 30 - 273 7.5e-101 NUBPL iron-transfer P-loop NTPase
MipZ PF09140 32 - 78 1.3e-06 ATPase MipZ
AAA_31 PF13614 33 - 72 2.4e-08 AAA domain
CbiA PF01656 34 - 201 1.4e-12 CobQ/CobB/MinD/ParA nucleotide binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 749
AccII CGCG 1 cut(s) 59
AciI CCGC 3 cut(s) 59, 347, 623
AclWI GGATC 2 cut(s) 670, 734
AcsI RAATTY 1 cut(s) 695
AcuI CTGAAG 1 cut(s) 288
AfeI AGCGCT 1 cut(s) 379
AfiI CCNNNNNNNGG 4 cut(s) 27, 187, 452, 587
AflIII ACRYGT 1 cut(s) 558
AgsI TTSAA 3 cut(s) 386, 513, 575
AjiI CACGTC 1 cut(s) 805
AjnI CCWGG 1 cut(s) 445
AloI GAACNNNNNNTCC 2 cut(s) 210, 242
AluBI AGCT 5 cut(s) 104, 244, 297, 609, 770
AluI AGCT 5 cut(s) 104, 244, 297, 609, 770
Alw26I GTCTC 1 cut(s) 790
AlwI GGATC 2 cut(s) 670, 734
AlwNI CAGNNNCTG 2 cut(s) 680, 773
Aor51HI AGCGCT 1 cut(s) 379
ApeKI GCWGC 2 cut(s) 10, 484
ApoI RAATTY 1 cut(s) 695
AseI ATTAAT 1 cut(s) 533
AspLEI GCGC 3 cut(s) 59, 380, 404
AspS9I GGNCC 3 cut(s) 70, 218, 362
AsuHPI GGTGA 1 cut(s) 467
AvaII GGWCC 3 cut(s) 70, 218, 362
BbvI GCAGC 1 cut(s) 471
BccI CCATC 1 cut(s) 679
BciT130I CCWGG 1 cut(s) 447
BciVI GTATCC 1 cut(s) 484
BcoDI GTCTC 1 cut(s) 790
BfaI CTAG 3 cut(s) 27, 375, 396
BfmI CTRYAG 1 cut(s) 144
BfoI RGCGCY 2 cut(s) 381, 405
BfuI GTATCC 1 cut(s) 484
BisI GCNGC 3 cut(s) 11, 60, 485
BlsI GCNGC 3 cut(s) 12, 61, 486
Bme1390I CCNGG 1 cut(s) 447
Bme18I GGWCC 3 cut(s) 70, 218, 362
BmgBI CACGTC 1 cut(s) 805
BmgT120I GGNCC 3 cut(s) 70, 218, 362
BmiI GGNNCC 2 cut(s) 364, 739
BmrFI CCNGG 1 cut(s) 447
BmrI ACTGGG 1 cut(s) 418
BmsI GCATC 4 cut(s) 193, 331, 448, 529
BmuI ACTGGG 1 cut(s) 418
BpuEI CTTGAG 1 cut(s) 504
BsaBI GATNNNNATC 1 cut(s) 287
BsaJI CCNNGG 1 cut(s) 366
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 187, 452, 587
Bse1I ACTGG 2 cut(s) 413, 457
Bse8I GATNNNNATC 1 cut(s) 287
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 1 cut(s) 366
BseGI GGATG 2 cut(s) 346, 544
BseJI GATNNNNATC 1 cut(s) 287
BseLI CCNNNNNNNGG 4 cut(s) 27, 187, 452, 587
BseMII CTCAG 1 cut(s) 672
BseNI ACTGG 2 cut(s) 413, 457
BseRI GAGGAG 1 cut(s) 51
BseXI GCAGC 1 cut(s) 471
Bsh1236I CGCG 1 cut(s) 59
BslFI GGGAC 3 cut(s) 336, 348, 737
BslI CCNNNNNNNGG 4 cut(s) 27, 187, 452, 587
BsmAI GTCTC 1 cut(s) 790
BsmFI GGGAC 3 cut(s) 336, 348, 737
Bsp143I GATC 2 cut(s) 662, 726
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 3 cut(s) 59, 347, 623
BspCNI CTCAG 1 cut(s) 673
BspFNI CGCG 1 cut(s) 59
BspLI GGNNCC 2 cut(s) 364, 739
BspMAI CTGCAG 1 cut(s) 148
BspPI GGATC 2 cut(s) 670, 734
BsrI ACTGG 2 cut(s) 413, 457
BssECI CCNNGG 1 cut(s) 366
BssMI GATC 2 cut(s) 662, 726
BssT1I CCWWGG 1 cut(s) 366
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 3 cut(s) 472, 629, 714
BstC8I GCNNGC 2 cut(s) 96, 197
BstDEI CTNAG 1 cut(s) 681
BstDSI CCRYGG 1 cut(s) 366
BstENI CCTNNNNNAGG 1 cut(s) 25
BstF5I GGATG 2 cut(s) 346, 544
BstFNI CGCG 1 cut(s) 59
BstH2I RGCGCY 2 cut(s) 381, 405
BstHHI GCGC 3 cut(s) 59, 380, 404
BstKTI GATC 2 cut(s) 665, 729
BstMAI GTCTC 1 cut(s) 790
BstMBI GATC 2 cut(s) 662, 726
BstNI CCWGG 1 cut(s) 447
BstNSI RCATGY 2 cut(s) 98, 562
BstSCI CCNGG 1 cut(s) 445
BstSFI CTRYAG 1 cut(s) 144
BstUI CGCG 1 cut(s) 59
BstV1I GCAGC 1 cut(s) 471
BstX2I RGATCY 1 cut(s) 662
BstYI RGATCY 1 cut(s) 662
BsuI GTATCC 1 cut(s) 484
BtgI CCRYGG 1 cut(s) 366
BtrI CACGTC 1 cut(s) 805
BtsCI GGATG 2 cut(s) 346, 544
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 3 cut(s) 141, 450, 625
Cac8I GCNNGC 2 cut(s) 96, 197
CaiI CAGNNNCTG 2 cut(s) 680, 773
CfoI GCGC 3 cut(s) 59, 380, 404
Cfr13I GGNCC 3 cut(s) 70, 218, 362
CviAII CATG 4 cut(s) 95, 367, 559, 604
DdeI CTNAG 1 cut(s) 681
DpnI GATC 2 cut(s) 664, 728
DpnII GATC 2 cut(s) 662, 726
DrdI GACNNNNNNGTC 1 cut(s) 749
DseDI GACNNNNNNGTC 1 cut(s) 749
Eco130I CCWWGG 1 cut(s) 366
Eco47I GGWCC 3 cut(s) 70, 218, 362
Eco47III AGCGCT 1 cut(s) 379
Eco57I CTGAAG 1 cut(s) 288
EcoNI CCTNNNNNAGG 1 cut(s) 25
EcoO109I RGGNCCY 2 cut(s) 70, 362
EcoRII CCWGG 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 4 cut(s) 98, 370, 562, 607
FalI AAGNNNNNCTT 2 cut(s) 179, 211
FaqI GGGAC 3 cut(s) 336, 348, 737
FatI CATG 4 cut(s) 94, 366, 558, 603
FauNDI CATATG 1 cut(s) 796
Fnu4HI GCNGC 3 cut(s) 11, 60, 485
FokI GGATG 2 cut(s) 353, 551
Fsp4HI GCNGC 3 cut(s) 11, 60, 485
FspBI CTAG 3 cut(s) 27, 375, 396
GlaI GCGC 3 cut(s) 58, 379, 403
GluI GCNGC 3 cut(s) 11, 60, 485
HaeII RGCGCY 2 cut(s) 381, 405
HhaI GCGC 3 cut(s) 59, 380, 404
Hin1II CATG 4 cut(s) 98, 370, 562, 607
Hin6I GCGC 3 cut(s) 57, 378, 402
HinP1I GCGC 3 cut(s) 57, 378, 402
HindIII AAGCTT 1 cut(s) 242
HinfI GANTC 3 cut(s) 283, 303, 776
HphI GGTGA 1 cut(s) 467
Hpy166II GTNNAC 3 cut(s) 138, 802, 854
Hpy188I TCNGA 6 cut(s) 78, 731, 742, 781, 813, 869
Hpy188III TCNNGA 7 cut(s) 292, 383, 419, 521, 652, 773, 787
Hpy8I GTNNAC 3 cut(s) 138, 802, 854
HpyAV CCTTC 7 cut(s) 31, 61, 83, 181, 652, 728, 829
HpyCH4III ACNGT 3 cut(s) 472, 629, 714
HpyCH4IV ACGT 1 cut(s) 804
HpyCH4V TGCA 4 cut(s) 146, 164, 487, 500
HpyF3I CTNAG 1 cut(s) 681
HpySE526I ACGT 1 cut(s) 804
Hsp92II CATG 4 cut(s) 98, 370, 562, 607
HspAI GCGC 3 cut(s) 57, 378, 402
Kzo9I GATC 2 cut(s) 662, 726
LmnI GCTCC 3 cut(s) 349, 743, 775
LpnPI CCDG 9 cut(s) 99, 140, 270, 394, 432, 438, 459, 480, 786
Lsp1109I GCAGC 1 cut(s) 471
LweI GCATC 4 cut(s) 193, 331, 448, 529
MaeI CTAG 3 cut(s) 27, 375, 396
MaeII ACGT 1 cut(s) 804
MaeIII GTNAC 1 cut(s) 262
MalI GATC 2 cut(s) 664, 728
MboI GATC 2 cut(s) 662, 726
MboII GAAGA 2 cut(s) 281, 851
MflI RGATCY 1 cut(s) 662
MluCI AATT 4 cut(s) 488, 552, 591, 695
MlyI GAGTC 1 cut(s) 312
MmeI TCCRAC 2 cut(s) 13, 765
MnlI CCTC 9 cut(s) 29, 32, 57, 76, 353, 453, 528, 542, 653
MseI TTAA 5 cut(s) 90, 150, 533, 555, 615
MslI CAYNNNNRTG 1 cut(s) 859
MspR9I CCNGG 1 cut(s) 447
MvaI CCWGG 1 cut(s) 447
MvnI CGCG 1 cut(s) 59
NcoI CCATGG 1 cut(s) 366
NdeI CATATG 1 cut(s) 796
NdeII GATC 2 cut(s) 662, 726
NlaIII CATG 4 cut(s) 98, 370, 562, 607
NlaIV GGNNCC 2 cut(s) 364, 739
NmuCI GTSAC 1 cut(s) 262
NspI RCATGY 2 cut(s) 98, 562
PaeI GCATGC 1 cut(s) 98
PciI ACATGT 1 cut(s) 558
PfeI GAWTC 2 cut(s) 283, 776
PkrI GCNGC 3 cut(s) 12, 61, 486
PleI GAGTC 1 cut(s) 311
PpsI GAGTC 1 cut(s) 311
PpuMI RGGWCCY 2 cut(s) 70, 362
PscI ACATGT 1 cut(s) 558
PshBI ATTAAT 1 cut(s) 533
Psp5II RGGWCCY 2 cut(s) 70, 362
Psp6I CCWGG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 2 cut(s) 364, 739
PspPI GGNCC 3 cut(s) 70, 218, 362
PspPPI RGGWCCY 2 cut(s) 70, 362
PstI CTGCAG 1 cut(s) 148
PstNI CAGNNNCTG 2 cut(s) 680, 773
PsuI RGATCY 1 cut(s) 662
RseI CAYNNNNRTG 1 cut(s) 859
SaqAI TTAA 5 cut(s) 90, 150, 533, 555, 615
SatI GCNGC 3 cut(s) 11, 60, 485
Sau3AI GATC 2 cut(s) 662, 726
Sau96I GGNCC 3 cut(s) 70, 218, 362
SchI GAGTC 1 cut(s) 312
ScrFI CCNGG 1 cut(s) 447
SfaNI GCATC 4 cut(s) 193, 331, 448, 529
SfcI CTRYAG 1 cut(s) 144
SinI GGWCC 3 cut(s) 70, 218, 362
SmiMI CAYNNNNRTG 1 cut(s) 859
SmlI CTYRAG 1 cut(s) 519
SmoI CTYRAG 1 cut(s) 519
SphI GCATGC 1 cut(s) 98
Sse9I AATT 4 cut(s) 488, 552, 591, 695
SsiI CCGC 3 cut(s) 59, 347, 623
SspMI CTAG 3 cut(s) 27, 375, 396
StyD4I CCNGG 1 cut(s) 445
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 3 cut(s) 472, 629, 714
TaiI ACGT 1 cut(s) 807
TaqI TCGA 3 cut(s) 291, 788, 833
TasI AATT 4 cut(s) 488, 552, 591, 695
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 2 cut(s) 283, 776
Tru1I TTAA 5 cut(s) 90, 150, 533, 555, 615
Tru9I TTAA 5 cut(s) 90, 150, 533, 555, 615
TscAI CASTG 3 cut(s) 148, 457, 632
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 2 cut(s) 10, 484
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 4 cut(s) 17, 200, 254, 634
TspGWI ACGGA 1 cut(s) 231
TspRI CASTG 3 cut(s) 148, 457, 632
VpaK11BI GGWCC 3 cut(s) 70, 218, 362
VspI ATTAAT 1 cut(s) 533
XagI CCTNNNNNAGG 1 cut(s) 25
XapI RAATTY 1 cut(s) 695
XceI RCATGY 2 cut(s) 98, 562
XcmI CCANNNNNNNNNTGG 1 cut(s) 188
XspI CTAG 3 cut(s) 27, 375, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.