MD10G1289100.v1.1

HB1, ASXL, restriction endonuclease HTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011708
Physical Location & Seq
Forward (+)
37820341 .. 37830401
10061 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1289100.v1.1.491

Sequence Viewer

Length: 5355 bp
ATGGAGGCGGCATCGGAGGGAGAAAATCAGAACAAAAATCATGAGAGCAATAGTAAATTTAACAATTCCAGTGAGGGGCAGAGCAAGCCCAAGCGTCAGATGAAGACCCCCTTTCAGCTCGAAACCCTCGAGAAAGCATATGCCTTGGATACCTACCCTTCGGAGGCGGTTAGGGCTGAGCTATCGGAAAGATTAGGGCTTTCCGATCGGCAGTTGCAGATGTGGTTCTGTCACAGAAGGTTGAAGGACAAGAAGGAAGGGGGTCCACCCAAGAAGCAGCGGAAATTGGCACCCCCTTTGCCTGAGCCTCCTATGGATGATTTGGCTCATGGCTCAGAGCCGGGTAGTGATTATGGGTCGGGCTCTGGCTCCGGCTCAAGCCCCTTTGGTCATGCACAGTTGCGGAATGTGGTGGCTAGGGGTGTGGCTGATGACATTCCAATGAGGGGGAGGAGGTATTATGAGTCGCCGCAGTCGAAATTGGAGCTCAGAGCCATTGCTTGTGTGGAAGCTCAGTTGGGAGAACCATTGAGGGAAAATGGCCCGCTTCTTGGGATAGAGTTTGATCCTTTGCCGCCAGATGCATTTGGGGCACCTATAGTTGCGGAGCAGCAGAAGCGGTCTGCCCATGCTTTGGAGGGCAAATATGAGCGTCATGATGCAAAACCAAATAAAGCTGCTCCAAGGACCCTCCATGAATATCCCTTTCTTCAAGATCACTCCAGTATTCGGTCTGATGCATATGGACAAGTCTCTCAATCTCATTTTCACGATTCAGCCATTGATGGTCCATCTGCAAGAACTTCATCATTTGCTGTTGGAAATGAGCCGTTGTCAAGGGTTCATGTCCATGGTCATGCATCGCGTGTTCGTCTTCTGTCTCAGCAGGAGAGGCAAGCGGTGCCCTACTCATCTCCTGTTGATGATGGAAGCGTACCACAAAGGGACTCCTTCACTAACGTAAGAGTAAATACTCAGTTTAGTGATCCCCCTGTTGTTGCTCCGGAAAACTCTAATTTATTATCTGAAGATCAAATTAATGACTCTATTCTGAGAATGGAGAGGAAACGCAAGAGTGAAGAAGTGAGAATCGCTAAAGAAGTTGAAGCTCATGAGCTGCGGATCCGGAAGGAGCTGGAGAAACAAGATATTCTGAGGAGAAAGAATGAGGAACGAATGAGGAAGGAAATGGAAAGGCAAGACCGTGAGAGACGAAAAGAAGAAGAGAGGTTGATGCGTGAAAGGCAGCGCGAGGAAGAGAGATTAAAGCGTGAGCAAAAACGTGAAATTGAACGAAGGGAAAAGTTTTTACAGAAAGAGTATATAAGAGCTGAGAAAAGGAGGCAGAAGGAAGAGCTTCGCAAAGAGAGAGAGGTAGTGAGACGCAAAGCTGCACTTGAGAAGGCAACTGCACGCAGGCTTGCTAAAGAATCTATGGAGCTTATTGAGGATGAGCAACTAGAACTAATGGAGTTGGCAGCTGCAAGCAAGGGTTTGTCCTCCATTATTTCTATTGATCTTGACACCCTGCAAAACCTTGATGCATTCAGAGATTCTTTGGTTGCATTCCCACCTAGGTCTGTGCAATTAAAAAGACCATTTGCAATTCAGCCGTGGATCAATTCAGAGGCGAATATTGGTAACCTTCTCATGGTTTGGAGATTTTTGATTACCTTCGCTGATGTTCTCGAGTTATGGCCCTTTACCCTTGATGAGTTTGTTCAAGCATTTCATGACTATGATTCAAGGTTGTTAGGAGAGATCCATGTTGCTCTCTTGAGGTTGATTATCAAAGATATTGAAGATGTTGCAAGGACTCCTACTGGATTAGGAATAAATCAAAATGGTGCTGCTAATCCTGGAGGTGGACATCCACAGATTGTTGAAGGAGCATATGCATGGGGCTTTGATATACGAAACTGGCAGCAGCACTTAAATCTGCTAACATGGCCTGAAATATTCCGGCAACTAGCACTCTCTGCCGGATTTGGGCCACAGCTGAAGAAAAGGAGTATTGCATGGTCATACACACCCGATAAGGATGAGGGTAAAGGTTGTCAGGATGCCATTTCTAATCTACGAAATGGTTCCGCAGCTGTAAATGCATTTGCAGTAATGCAAGAGAAAGGCTTACTGGCTCCAAGAAGATCTAGACACCGGTTGACTCCTGGAACTGTAAAGTTTGCAGCTTTTCATGTGCTTTCACTTGAGGGAAATAAGGGACTAACAGTGTTAGATCTTGCAGAAAAGATTCAGAAATCTGGCCTTCGGGACCTGACAACAAGCAAGACTCCTGAAGCTTCAATTTCTGTAGCTTTGACAAGAGATACTAAGCTCTTTGAAAGAATAGCTCCTTCAACATATCGTGTACGAGCTGCTTACAGAAAGGATCCTATTGATGCTGAGGCTGTACTTTCAGCGGCCAGGAAGAAAATTCAGATATTTGAAAATGGAATTTTAGCTGCAGAAGATGTCGATGATGTTGAAAGAGATGATGTTGAAAATGATGAGGTTGAAAGAGACGAAGACTTTGAATGTGAAGTTGATGAAGATCCTGAAGTTGATGACTTAGCTACTCCATCAGTTGCAAAGAAATCCCCTGATGATTACAGTGAAGTAATTATTTTCTCAGAAAATGGAAAGGACAGTTTATGCGGCGATGTTGGACTTGCTGTGAAAAATGAGTTTGATAAGGATGTTTTATGTTCCCCCGTCACTGGTTCCAAAGATGTATTCTGTCCTAGTGCTTCCTCAAAACAGTGTGTTTCAGGTGTAGATATTAGTACTAGTAATCTTGACCAAGAAAATATGGAGATTGATGAAAGCAAGTCAGGTGAGTCATGGGTTCAAGGACTTACAGAAGGGGAATATTCTGATCTCAGCGTGGAAGAGCGTCTTAGAGGTCTTGTTGCCTTAATTGGTGTTGCAAATGAAGGAAACACCATTCGTGTTGTTCTCGAGGATCGCTTAGAAGCTGCAAATGCTCTTAAGAAGCAAATGTTGGCAGAGGCACAGCTGGATAAAAGTCGCCTAAAAGAAGAGAATGTTGGTAGGTTAGATTTTCCATCTTTTATGGGAGGCAAAGCTGAAGTACAAGCTACCGGAGTAGAGGACGGCCAAAGTCCCTTACGGGATGTTGATAACAGAAACATTGAAGCATCTCCAGTCACTGCAGAAAATCAAAAGTCTATCCATGGGTCACAAGGTGTTCAGAATCAGCTAAATGGTGTGCCTGTTGAAAGGACCTCAGCAGCCCAAGATTTCTCCATGGGTTCTGATAACTTTTTGTCTCAGCAACTTGCATATGCATCAAAAAGGTCACGCTCACAGTTAAAATCATATATTGCACATAGAGCAGAAGAGATGTATGCATACAGGTCCCTGCCTCTTGGTCAAGATCGTAGGCATAATCGATACTGGCAATTTGTTGCATCTGCATCAAGAAATGATCCTGGTTCAGGCAGAATTTTTATTGAATTGAACAATGGAAGCTGGAGGCTTATTGACACTGAAGGGGCCTTTGATGCTCTTGTTACGGCTCTGGACACACGCGGGATTAGGGAATCTCATTTACGCTTAATGTTGCAAAAGATTGAGGCATCCTTCAGGGAGAATGGTCGGAAAAATGCACATTGTCCTAACCATGCAGGCCCGAGTAAAAACCATGTTAAAAATGAAGCCGACGAAATGGATTCTAGTCCTGATTTCCCTTCTCGTTTTGACAGCCCTGAAAGTACAGTCTGTGCTTTGAATTCTGATACAACGCAGAGCTCCTCTTCTTTCAGAATTGAGCTCGACAGAAGTGAAACTGAGAAAAGGGCTGCCTTGAGAAGGTATCAAGATTTTCAGAAGTGGATGTGGAAAGAATGCTTTAATTCATCTACCTTATGTGCCACGAAATATGGGAAGGAGAGATGCAGACCGCTGTTTGATGTTTGTGATGTCTGCCTCAGTTGTTATTACTTTGAGGATTCCCACTGCGATACTTGCCATCAGACTTTTGATGCCTTCAACGGAAATTTTGACTTTAATGAACATGTAATTCAATGTAAAAGAAAAAGGACGTTGGAACCTTGGGATTTCCATATTCCTTGTACTTCTGTTCCCTTGGCCAGGAGATTGCTCAAGGCATTAATAGGTCTTGTTGAGGTATGTACCCCACCAGAGGCTCTTCAATCAGTTTGGATTGAAGACCGTCGAAAGATGTGGGGAGAGAAACTGAATGCGTCATCATCAATGGAAGAACTTCTACAGACATTGACTCTGTTTGAGACTGCTGTGAAGAAAGACTTTCTGTCATCACACTTTGCACTGACGGAGGAATTGCTGGGCTCCTGCAAACAGCTGGGGGTTGTTCGCGATTATCTTGACAATGGATCCGTTCCTGTACTTCCTTGGATACCACTTACCACTGCAGCCGTGGCTTTGAGGCTTCATGAGATAGATTCAGCAATTACATATGTAAAGCAAGCGACACCTGAGCCCACCGCGGACAAGGAAGTGGGAGAATATCTTAATCAACCTATGAGACTTCTGGCATCGAGGAGTGAGACTGAACTAACAGAAGCAGGCCTCAATGAGTACACGAGGGAAGACAACAGTACTCACCTGAAAAGTGGACGAAACAGCTTTAGACGTGGTAGAGGAGGTCGAGAGCAAGGACGCGGTAAAAGGTGGCAGAAAAAGGTGAATAATCCCAAATCAGTGGCAAGTCGTCGGAACATTAGGGGTAATGAGAATTTGAGTCAAGGGTTGAGAACTGTGGGAAAACGAACGCAGGGACAAGGGAGTGGACGGGGTCGCCGAACTGTTAGAAAGAGGAGAATGAAGAACAGGGCTGTTGAGGGGACACCTCTGGGCCGTATGAGTGACATACGCAGCAGCCCTGAGAGTGGAGGAGAATCGCCAAGAAACTTGGCTGAGGAATGGGATGATGAAAAGATCGATATGAAAGGTGACGAGCAAGGAGAGGGATATGAACAAGGGGAGTTGGGAGAGGGATATGAACAAGGAGAGTTGGGAGAGGGATATGAACAAGGAGAGTTAGGAGAAGGATATGAACAAGGAGGCGCATTGGAATCTGAAGACGACGAACAAGCAGTGGGATATGGACAAGGTAACTGGGAAATTGGGTTTGATGGGACTTCCAGCGGATGGAACAGGGGTTTAGTAGAAGCTAGTGACAACGATTTGGATACTTCTGAAGATGACAATAACGTCATGGAAGAAGTGAGAGACGAAGATTCAGAAGGGGATGTAGATGTCGATATGAGTGATGCCTCGGATGAAATGCCAAACGCGATGAGAAACGATGATGGCACAGAGTCGGAAAGTTCAGATGACTATAGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000122 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001077 GO:0001227 GO:0001228 GO:0001525 GO:0001568 GO:0001944 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030154 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0035239 GO:0035295 GO:0040034 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048506 GO:0048509 GO:0048510 GO:0048514 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0072358 GO:0072359 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1990837 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1785

Amino Acids

200.69

Weight (kDa)

5.28

Isoelectric Point (pI)

52.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 29 - 85 1e-16 Homeodomain
DDT PF02791 545 - 599 3e-20 DDT domain
HARE-HTH PF05066 724 - 792 2e-18 HB1, ASXL, restriction endonuclease HTH domain
WHIM1 PF15612 943 - 987 7.2e-07 WSTF, HB1, Itc1p, MBD9 motif 1
WSD PF15613 1125 - 1198 9.4e-21 Williams-Beuren syndrome DDT (WSD), D-TOX E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011708)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28420
fragaria_vesca FvH4_3g08620
malus_domestica MD05G1310200.v1.1 MD10G1289100.v1.1
prunus_persica Prupe.4G052600_v2.0.a1
pyrus_communis pycom05g28660 pycom10g24170
rosa_chinensis RchiOBHm_Chr5g0009221
rosa_laevigata RLG00000031635 RLG00000031641
rosa_multiflora Rmu_sc0006233.1_g000030
rosa_roxburghii Rroxscaffold_1G00066840 Rroxscaffold_1G00067540
rosa_rugosa Rorug04G0441600
rosa_samantha Rh5AG071400 Rh5BG067900 Rh5CG080200 Rh5DG067900
rosa_wichuraiana Rw5G006670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 5216
AccB1I GGYRCC 3 cut(s) 289, 592, 901
AccB7I CCANNNNNTGG 2 cut(s) 634, 5154
AccII CGCG 7 cut(s) 865, 1251, 3552, 4359, 4490, 4665, 5300
AccIII TCCGGA 2 cut(s) 1003, 1125
AcoI YGGCCR 3 cut(s) 2421, 3115, 4110
AcsI RAATTY 7 cut(s) 56, 2433, 2454, 3465, 3751, 4018, 4738
AcuI CTGAAG 9 cut(s) 1047, 2021, 2316, 2577, 3108, 3531, 3589, 5103, 5223
AdeI CACNNNGTG 1 cut(s) 3206
AflII CTTAAG 1 cut(s) 2987
AflIII ACRYGT 1 cut(s) 4036
AgeI ACCGGT 1 cut(s) 2157
AhdI GACNNNNNGTC 1 cut(s) 4294
AhlI ACTAGT 1 cut(s) 2786
AjiI CACGTC 1 cut(s) 4637
AjnI CCWGG 5 cut(s) 1858, 2167, 2423, 3451, 4112
AjuI GAANNNNNNNTTGG 6 cut(s) 2717, 2749, 2984, 3016, 3030, 3062
Alw21I GWGCWC 3 cut(s) 489, 3773, 3795
AlwNI CAGNNNCTG 1 cut(s) 3170
Ama87I CYCGRG 4 cut(s) 128, 1688, 2957, 3652
Aor13HI TCCGGA 2 cut(s) 1003, 1125
ApoI RAATTY 7 cut(s) 56, 2433, 2454, 3465, 3751, 4018, 4738
ArsI GACNNNNNNTTYG 2 cut(s) 2513, 2545
AseI ATTAAT 2 cut(s) 1038, 4133
AsiGI ACCGGT 1 cut(s) 2157
Asp700I GAANNNNTTC 4 cut(s) 1356, 2086, 2250, 4245
AspA2I CCTAGG 1 cut(s) 1574
AspLEI GCGC 2 cut(s) 1251, 5072
AsuC2I CCSGG 1 cut(s) 342
AsuHPI GGTGA 4 cut(s) 2846, 4598, 4699, 4967
AvaI CYCGRG 4 cut(s) 128, 1688, 2957, 3652
AvaII GGWCC 6 cut(s) 263, 687, 788, 2272, 3243, 3378
AvrII CCTAGG 1 cut(s) 1574
BaeGI GKGCMC 2 cut(s) 595, 906
BalI TGGCCA 1 cut(s) 4112
BamHI GGATCC 3 cut(s) 1122, 2389, 4376
BanI GGYRCC 3 cut(s) 289, 592, 901
BanII GRGCYC 6 cut(s) 365, 489, 3773, 3795, 4334, 4485
BarI GAAGNNNNNNTAC 2 cut(s) 4233, 4265
BauI CACGAG 1 cut(s) 4585
BbsI GAAGAC 6 cut(s) 110, 866, 2532, 4197, 4599, 5091
Bbv12I GWGCWC 3 cut(s) 489, 3773, 3795
BbvCI CCTCAGC 3 cut(s) 2403, 3247, 4920
BccI CCATC 9 cut(s) 779, 799, 920, 2587, 3073, 3999, 5132, 5148, 5309
BceAI ACGGC 6 cut(s) 814, 1597, 3130, 3552, 4403, 4845
BciT130I CCWGG 5 cut(s) 1860, 2169, 2425, 3453, 4114
BciVI GTATCC 3 cut(s) 142, 4392, 5188
BcnI CCSGG 1 cut(s) 342
BcuI ACTAGT 1 cut(s) 2786
BfaI CTAG 9 cut(s) 417, 1460, 1575, 1970, 2151, 2742, 2787, 3696, 5178
BfmI CTRYAG 7 cut(s) 597, 2310, 2463, 3171, 4250, 4413, 5344
BfrI CTTAAG 1 cut(s) 2987
BfuI GTATCC 3 cut(s) 142, 4392, 5188
BglII AGATCT 2 cut(s) 2147, 2236
BlnI CCTAGG 1 cut(s) 1574
BlpI GCTNAGC 1 cut(s) 177
BmcAI AGTACT 2 cut(s) 2785, 4603
Bme1390I CCNGG 6 cut(s) 342, 1860, 2169, 2425, 3453, 4114
Bme18I GGWCC 6 cut(s) 263, 687, 788, 2272, 3243, 3378
BmeRI GACNNNNNGTC 1 cut(s) 4294
BmeT110I CYCGRG 4 cut(s) 128, 1688, 2957, 3652
BmgBI CACGTC 1 cut(s) 4637
BmrFI CCNGG 6 cut(s) 342, 1860, 2169, 2425, 3453, 4114
BmrI ACTGGG 1 cut(s) 5131
BmuI ACTGGG 1 cut(s) 5131
BpiI GAAGAC 6 cut(s) 110, 866, 2532, 4197, 4599, 5091
BpmI CTGGAG 5 cut(s) 706, 1157, 1881, 3147, 3514
Bpu10I CCTNAGC 5 cut(s) 303, 2403, 3247, 4479, 4920
Bpu1102I GCTNAGC 1 cut(s) 177
BpuEI CTTGAG 6 cut(s) 361, 1418, 1798, 2228, 3847, 4109
BpuMI CCSGG 1 cut(s) 342
Bsa29I ATCGAT 2 cut(s) 3412, 4944
BsaBI GATNNNNATC 1 cut(s) 2550
BsaWI WCCGGW 4 cut(s) 1003, 1125, 2157, 3101
Bse118I RCCGGY 1 cut(s) 2157
Bse1I ACTGG 9 cut(s) 69, 723, 1828, 1925, 2139, 2722, 3164, 3422, 5126
Bse3DI GCAATG 1 cut(s) 495
Bse8I GATNNNNATC 1 cut(s) 2550
BseAI TCCGGA 2 cut(s) 1003, 1125
BseBI CCWGG 5 cut(s) 1860, 2169, 2425, 3453, 4114
BseCI ATCGAT 2 cut(s) 3412, 4944
BseJI GATNNNNATC 1 cut(s) 2550
BseMI GCAATG 1 cut(s) 495
BseNI ACTGG 9 cut(s) 69, 723, 1828, 1925, 2139, 2722, 3164, 3422, 5126
BseRI GAGGAG 7 cut(s) 466, 1171, 3763, 4558, 4659, 4834, 4911
BseSI GKGCMC 2 cut(s) 595, 906
BseYI CCCAGC 2 cut(s) 4327, 4345
BsgI GTGCAG 2 cut(s) 1377, 1395
Bsh1236I CGCG 7 cut(s) 865, 1251, 3552, 4359, 4490, 4665, 5300
Bsh1285I CGRYCG 1 cut(s) 208
BshNI GGYRCC 3 cut(s) 289, 592, 901
BshTI ACCGGT 1 cut(s) 2157
BshVI ATCGAT 2 cut(s) 3412, 4944
BsiEI CGRYCG 1 cut(s) 208
BsiHKAI GWGCWC 3 cut(s) 489, 3773, 3795
BsiHKCI CYCGRG 4 cut(s) 128, 1688, 2957, 3652
BsiSI CCGG 8 cut(s) 341, 372, 1004, 1126, 1963, 1983, 2158, 3102
BslFI GGGAC 8 cut(s) 959, 2235, 2285, 3108, 3364, 4794, 4861, 5155
BsmBI CGTCTC 4 cut(s) 1204, 1375, 2514, 5229
BsmFI GGGAC 8 cut(s) 959, 2235, 2285, 3108, 3364, 4794, 4861, 5155
BsmI GAATGC 4 cut(s) 1544, 1565, 3872, 4228
BsoBI CYCGRG 4 cut(s) 128, 1688, 2957, 3652
Bsp1286I GDGCHC 8 cut(s) 365, 489, 595, 906, 3773, 3795, 4334, 4485
Bsp13I TCCGGA 2 cut(s) 1003, 1125
Bsp1720I GCTNAGC 1 cut(s) 177
Bsp19I CCATGG 3 cut(s) 850, 3193, 3267
Bsp68I TCGCGA 1 cut(s) 4359
BspDI ATCGAT 2 cut(s) 3412, 4944
BspEI TCCGGA 2 cut(s) 1003, 1125
BspFNI CGCG 7 cut(s) 865, 1251, 3552, 4359, 4490, 4665, 5300
BspHI TCATGA 5 cut(s) 40, 655, 1111, 1732, 4435
BspMAI CTGCAG 3 cut(s) 2467, 3175, 4417
BspQI GCTCTTC 3 cut(s) 1347, 2883, 4176
BspT107I GGYRCC 3 cut(s) 289, 592, 901
BspTI CTTAAG 1 cut(s) 2987
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 1 cut(s) 2157
BsrI ACTGG 9 cut(s) 69, 723, 1828, 1925, 2139, 2722, 3164, 3422, 5126
BssAI RCCGGY 1 cut(s) 2157
BssSI CACGAG 1 cut(s) 4585
BssT1I CCWWGG 9 cut(s) 144, 683, 850, 1574, 3193, 3267, 4073, 4107, 4394
Bst2BI CACGAG 1 cut(s) 4585
Bst2UI CCWGG 5 cut(s) 1860, 2169, 2425, 3453, 4114
Bst6I CTCTTC 8 cut(s) 1218, 1251, 1347, 2883, 3033, 3354, 3781, 4176
BstAFI CTTAAG 1 cut(s) 2987
BstAPI GCANNNNNTGC 2 cut(s) 901, 1979
BstDSI CCRYGG 6 cut(s) 850, 1613, 3193, 3267, 4419, 4488
BstEII GGTNACC 1 cut(s) 1640
BstENI CCTNNNNNAGG 3 cut(s) 3456, 3829, 4111
BstFNI CGCG 7 cut(s) 865, 1251, 3552, 4359, 4490, 4665, 5300
BstHHI GCGC 2 cut(s) 1251, 5072
BstMCI CGRYCG 1 cut(s) 208
BstNI CCWGG 5 cut(s) 1860, 2169, 2425, 3453, 4114
BstNSI RCATGY 1 cut(s) 4040
BstPI GGTNACC 1 cut(s) 1640
BstSCI CCNGG 6 cut(s) 340, 1858, 2167, 2423, 3451, 4112
BstSFI CTRYAG 7 cut(s) 597, 2310, 2463, 3171, 4250, 4413, 5344
BstSLI GKGCMC 2 cut(s) 595, 906
BstUI CGCG 7 cut(s) 865, 1251, 3552, 4359, 4490, 4665, 5300
BstV2I GAAGAC 6 cut(s) 110, 866, 2532, 4197, 4599, 5091
BstX2I RGATCY 7 cut(s) 1122, 1761, 2147, 2236, 2389, 2551, 4376
BstXI CCANNNNNNTGG 1 cut(s) 4705
BstYI RGATCY 7 cut(s) 1122, 1761, 2147, 2236, 2389, 2551, 4376
Bsu15I ATCGAT 2 cut(s) 3412, 4944
BsuI GTATCC 3 cut(s) 142, 4392, 5188
BsuTUI ATCGAT 2 cut(s) 3412, 4944
BtgI CCRYGG 6 cut(s) 850, 1613, 3193, 3267, 4419, 4488
BtgZI GCGATG 3 cut(s) 846, 2673, 5315
BtrI CACGTC 1 cut(s) 4637
BtsI GCAGTG 4 cut(s) 3168, 3976, 4410, 5106
BtuMI TCGCGA 1 cut(s) 4359
CaiI CAGNNNCTG 1 cut(s) 3170
CciI TCATGA 5 cut(s) 40, 655, 1111, 1732, 4435
CfoI GCGC 2 cut(s) 1251, 5072
Cfr10I RCCGGY 1 cut(s) 2157
Cfr42I CCGCGG 1 cut(s) 4491
ClaI ATCGAT 2 cut(s) 3412, 4944
CseI GACGC 6 cut(s) 83, 641, 1392, 2882, 4215, 4671
CspAI ACCGGT 1 cut(s) 2157
DraIII CACNNNGTG 1 cut(s) 3206
DrdI GACNNNNNNGTC 1 cut(s) 5216
DriI GACNNNNNGTC 1 cut(s) 4294
DseDI GACNNNNNNGTC 1 cut(s) 5216
EaeI YGGCCR 3 cut(s) 2421, 3115, 4110
Eam1104I CTCTTC 8 cut(s) 1218, 1251, 1347, 2883, 3033, 3354, 3781, 4176
Eam1105I GACNNNNNGTC 1 cut(s) 4294
EarI CTCTTC 8 cut(s) 1218, 1251, 1347, 2883, 3033, 3354, 3781, 4176
Ecl136II GAGCTC 3 cut(s) 487, 3771, 3793
Eco130I CCWWGG 9 cut(s) 144, 683, 850, 1574, 3193, 3267, 4073, 4107, 4394
Eco147I AGGCCT 1 cut(s) 4572
Eco24I GRGCYC 6 cut(s) 365, 489, 3773, 3795, 4334, 4485
Eco47I GGWCC 6 cut(s) 263, 687, 788, 2272, 3243, 3378
Eco53kI GAGCTC 3 cut(s) 487, 3771, 3793
Eco57I CTGAAG 9 cut(s) 1047, 2021, 2316, 2577, 3108, 3531, 3589, 5103, 5223
Eco88I CYCGRG 4 cut(s) 128, 1688, 2957, 3652
Eco91I GGTNACC 1 cut(s) 1640
EcoICRI GAGCTC 3 cut(s) 487, 3771, 3793
EcoNI CCTNNNNNAGG 3 cut(s) 3456, 3829, 4111
EcoO109I RGGNCCY 5 cut(s) 687, 2272, 3243, 3378, 3516
EcoO65I GGTNACC 1 cut(s) 1640
EcoRI GAATTC 1 cut(s) 3751
EcoRII CCWGG 5 cut(s) 1858, 2167, 2423, 3451, 4112
EcoT14I CCWWGG 9 cut(s) 144, 683, 850, 1574, 3193, 3267, 4073, 4107, 4394
EcoT22I ATGCAT 8 cut(s) 586, 742, 862, 1546, 1900, 2107, 3310, 3373
EcoT38I GRGCYC 6 cut(s) 365, 489, 3773, 3795, 4334, 4485
ErhI CCWWGG 9 cut(s) 144, 683, 850, 1574, 3193, 3267, 4073, 4107, 4394
Esp3I CGTCTC 4 cut(s) 1204, 1375, 2514, 5229
FalI AAGNNNNNCTT 8 cut(s) 95, 127, 1380, 1412, 2880, 2912, 4274, 4306
FaqI GGGAC 8 cut(s) 959, 2235, 2285, 3108, 3364, 4794, 4861, 5155
FauI CCCGC 2 cut(s) 552, 3545
FauNDI CATATG 5 cut(s) 139, 742, 1894, 3304, 4459
FriOI GRGCYC 6 cut(s) 365, 489, 3773, 3795, 4334, 4485
FspBI CTAG 9 cut(s) 417, 1460, 1575, 1970, 2151, 2742, 2787, 3696, 5178
GlaI GCGC 2 cut(s) 1250, 5071
GsaI CCCAGC 2 cut(s) 4331, 4349
GsuI CTGGAG 5 cut(s) 706, 1157, 1881, 3147, 3514
HapII CCGG 8 cut(s) 341, 372, 1004, 1126, 1963, 1983, 2158, 3102
HgaI GACGC 6 cut(s) 83, 641, 1392, 2882, 4215, 4671
HhaI GCGC 2 cut(s) 1251, 5072
Hin6I GCGC 2 cut(s) 1249, 5070
HinP1I GCGC 2 cut(s) 1249, 5070
HincII GTYRAC 1 cut(s) 2163
HindII GTYRAC 1 cut(s) 2163
HindIII AAGCTT 1 cut(s) 2298
HpaII CCGG 8 cut(s) 341, 372, 1004, 1126, 1963, 1983, 2158, 3102
HphI GGTGA 4 cut(s) 2846, 4598, 4699, 4967
Hpy166II GTNNAC 7 cut(s) 266, 1868, 2163, 2369, 4584, 4619, 4793
Hpy8I GTNNAC 7 cut(s) 266, 1868, 2163, 2369, 4584, 4619, 4793
Hpy99I CGWCG 4 cut(s) 3686, 4200, 4719, 5093
HpyCH4IV ACGT 5 cut(s) 960, 1282, 4064, 4636, 5217
HpySE526I ACGT 5 cut(s) 960, 1282, 4064, 4636, 5217
HspAI GCGC 2 cut(s) 1249, 5070
Kpn2I TCCGGA 2 cut(s) 1003, 1125
KspI CCGCGG 1 cut(s) 4491
LguI GCTCTTC 3 cut(s) 1347, 2883, 4176
MaeI CTAG 9 cut(s) 417, 1460, 1575, 1970, 2151, 2742, 2787, 3696, 5178
MaeII ACGT 5 cut(s) 960, 1282, 4064, 4636, 5217
MflI RGATCY 7 cut(s) 1122, 1761, 2147, 2236, 2389, 2551, 4376
MhlI GDGCHC 8 cut(s) 365, 489, 595, 906, 3773, 3795, 4334, 4485
MlsI TGGCCA 1 cut(s) 4112
MluNI TGGCCA 1 cut(s) 4112
MmeI TCCRAC 6 cut(s) 799, 2644, 3599, 4047, 4697, 5307
Mox20I TGGCCA 1 cut(s) 4112
Mph1103I ATGCAT 8 cut(s) 586, 742, 862, 1546, 1900, 2107, 3310, 3373
MroI TCCGGA 2 cut(s) 1003, 1125
MroXI GAANNNNTTC 4 cut(s) 1356, 2086, 2250, 4245
MscI TGGCCA 1 cut(s) 4112
MslI CAYNNNNRTG 5 cut(s) 440, 849, 855, 1737, 1897
Msp20I TGGCCA 1 cut(s) 4112
MspCI CTTAAG 1 cut(s) 2987
MspI CCGG 8 cut(s) 341, 372, 1004, 1126, 1963, 1983, 2158, 3102
MspR9I CCNGG 6 cut(s) 342, 1860, 2169, 2425, 3453, 4114
Mva1269I GAATGC 4 cut(s) 1544, 1565, 3872, 4228
MvaI CCWGG 5 cut(s) 1860, 2169, 2425, 3453, 4114
MvnI CGCG 7 cut(s) 865, 1251, 3552, 4359, 4490, 4665, 5300
NciI CCSGG 1 cut(s) 342
NcoI CCATGG 3 cut(s) 850, 3193, 3267
NdeI CATATG 5 cut(s) 139, 742, 1894, 3304, 4459
NmuCI GTSAC 8 cut(s) 230, 2713, 3166, 3198, 3318, 4868, 4955, 5180
NruI TCGCGA 1 cut(s) 4359
NsiI ATGCAT 8 cut(s) 586, 742, 862, 1546, 1900, 2107, 3310, 3373
NspI RCATGY 1 cut(s) 4040
PaeR7I CTCGAG 3 cut(s) 128, 1688, 2957
PagI TCATGA 5 cut(s) 40, 655, 1111, 1732, 4435
PceI AGGCCT 1 cut(s) 4572
PciI ACATGT 1 cut(s) 4036
PciSI GCTCTTC 3 cut(s) 1347, 2883, 4176
PcsI WCGNNNNNNNCGW 3 cut(s) 126, 473, 4801
PctI GAATGC 4 cut(s) 1544, 1565, 3872, 4228
PdmI GAANNNNTTC 4 cut(s) 1356, 2086, 2250, 4245
PflFI GACNNNGTC 1 cut(s) 4797
PflMI CCANNNNNTGG 2 cut(s) 634, 5154
PfoI TCCNGGA 2 cut(s) 1858, 2167
PinAI ACCGGT 1 cut(s) 2157
Ple19I CGATCG 1 cut(s) 208
PpuMI RGGWCCY 4 cut(s) 687, 2272, 3243, 3378
PscI ACATGT 1 cut(s) 4036
PshBI ATTAAT 2 cut(s) 1038, 4133
Psp124BI GAGCTC 3 cut(s) 489, 3773, 3795
Psp5II RGGWCCY 4 cut(s) 687, 2272, 3243, 3378
Psp6I CCWGG 5 cut(s) 1858, 2167, 2423, 3451, 4112
PspEI GGTNACC 1 cut(s) 1640
PspFI CCCAGC 2 cut(s) 4327, 4345
PspGI CCWGG 5 cut(s) 1858, 2167, 2423, 3451, 4112
PspPPI RGGWCCY 4 cut(s) 687, 2272, 3243, 3378
PstI CTGCAG 3 cut(s) 2467, 3175, 4417
PstNI CAGNNNCTG 1 cut(s) 3170
PsuI RGATCY 7 cut(s) 1122, 1761, 2147, 2236, 2389, 2551, 4376
PsyI GACNNNGTC 1 cut(s) 4797
PvuI CGATCG 1 cut(s) 208
PvuII CAGCTG 5 cut(s) 1481, 1999, 2096, 3016, 4345
RruI TCGCGA 1 cut(s) 4359
RseI CAYNNNNRTG 5 cut(s) 440, 849, 855, 1737, 1897
SacI GAGCTC 3 cut(s) 489, 3773, 3795
SacII CCGCGG 1 cut(s) 4491
SapI GCTCTTC 3 cut(s) 1347, 2883, 4176
ScaI AGTACT 2 cut(s) 2785, 4603
ScrFI CCNGG 6 cut(s) 342, 1860, 2169, 2425, 3453, 4114
SduI GDGCHC 8 cut(s) 365, 489, 595, 906, 3773, 3795, 4334, 4485
SfcI CTRYAG 7 cut(s) 597, 2310, 2463, 3171, 4250, 4413, 5344
Sfr274I CTCGAG 3 cut(s) 128, 1688, 2957
Sfr303I CCGCGG 1 cut(s) 4491
SgrBI CCGCGG 1 cut(s) 4491
SinI GGWCC 6 cut(s) 263, 687, 788, 2272, 3243, 3378
SlaI CTCGAG 3 cut(s) 128, 1688, 2957
SmiMI CAYNNNNRTG 5 cut(s) 440, 849, 855, 1737, 1897
SpeI ACTAGT 1 cut(s) 2786
SseBI AGGCCT 1 cut(s) 4572
SspI AATATT 3 cut(s) 1636, 1959, 2870
SspMI CTAG 9 cut(s) 417, 1460, 1575, 1970, 2151, 2742, 2787, 3696, 5178
SstI GAGCTC 3 cut(s) 489, 3773, 3795
StuI AGGCCT 1 cut(s) 4572
StyD4I CCNGG 6 cut(s) 340, 1858, 2167, 2423, 3451, 4112
StyI CCWWGG 9 cut(s) 144, 683, 850, 1574, 3193, 3267, 4073, 4107, 4394
TaiI ACGT 5 cut(s) 963, 1285, 4067, 4639, 5220
TaqII GACCGA 1 cut(s) 720
TatI WGTACW 8 cut(s) 2410, 2783, 3091, 3734, 4094, 4387, 4581, 4601
TauI GCSGC 5 cut(s) 11, 472, 577, 2423, 2658
TseFI GTSAC 8 cut(s) 230, 2713, 3166, 3198, 3318, 4868, 4955, 5180
Tsp45I GTSAC 8 cut(s) 230, 2713, 3166, 3198, 3318, 4868, 4955, 5180
TspGWI ACGGA 3 cut(s) 4029, 4331, 4369
Tth111I GACNNNGTC 1 cut(s) 4797
Van91I CCANNNNNTGG 2 cut(s) 634, 5154
Vha464I CTTAAG 1 cut(s) 2987
VpaK11BI GGWCC 6 cut(s) 263, 687, 788, 2272, 3243, 3378
VspI ATTAAT 2 cut(s) 1038, 4133
XagI CCTNNNNNAGG 3 cut(s) 3456, 3829, 4111
XapI RAATTY 7 cut(s) 56, 2433, 2454, 3465, 3751, 4018, 4738
XbaI TCTAGA 1 cut(s) 2150
XceI RCATGY 1 cut(s) 4040
XcmI CCANNNNNNNNNTGG 2 cut(s) 502, 4417
XhoI CTCGAG 3 cut(s) 128, 1688, 2957
XmaJI CCTAGG 1 cut(s) 1574
XmnI GAANNNNTTC 4 cut(s) 1356, 2086, 2250, 4245
XspI CTAG 9 cut(s) 417, 1460, 1575, 1970, 2151, 2742, 2787, 3696, 5178
ZrmI AGTACT 2 cut(s) 2785, 4603
Zsp2I ATGCAT 8 cut(s) 586, 742, 862, 1546, 1900, 2107, 3310, 3373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.