Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0011708

Physical Location & Seq

Genomic Coordinates Reverse (-)

6136163 .. 6145607

Gene Structure 9445 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ29011

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5202 bp

ATGGAGGGTTCGGAAGGAGATAATCAGAATCGGAACCCCGAGAATAGCAATAGTAAATTGAACAATTCCGGCGAGGGAGGCAAGCCCAAGCGCCAGATGAAGACGCCGTTTCAGCTCGAAACCCTAGAGAAGGCCTATGCGTTGGAGACGTACCCATCGGAGTCGATTAGGGCGGAGCTGTCGGAGAAATTGGGGCTTTCCGATCGGCAGTTGCAGATGTGGTTCTGTCACAGAAGGCTGAAGGATAAGAAGGAAGGAGGTTCGGGTTCGGGTCCGGGTCCGGGTCCAGGACCGGCGAAGAAGCAGAGGAAATCAGTGGCGGTGTTGCCGGAGCCTCCAATTGATGACTTGGCACATGGGTCGGAGCCGGGGAGTGACTATGGGTCGGGTTCGGGCTCTGGCTCGAGCCCATTTGGTCATGCAGACCGCAATGTGGTGTCCAGGAGTGTTGTTGTTGAGGATATGCCAAGGAGGAGGCATTATGAGTCACAGCAGTCGATTACGGAGCTTAGAGCCATTGCTATTGTGGAAGCTCAATTGGGAGAGCCATTAAGGGAAGATGGCCCTGTGCTTGGGATAGAGTTTGATCAATTGCCCCCGGATGCATTCGGGGCACCTCTAGTTGCAGAGCAGCAGAAGCGGCATGATGCAAAACTAAATAAAGGTACTCCGAGATCTCTCCATGAATATCCATATCTTCAAGACCACCCCATTATTCGGCCTGATGTATATGGACAAGTTGCTCAATCTCATTTTCATGATTCAGCAATTGATGGTCCAACTGCAAGAGCTTCACCATTTGCAAGTGGAAATGAACAGTTGTCAAGGGTTCACGGTGGCCACAGTCGGGCTCGTCTTCTGTCACAACAGGAGAAGCAAGCAGTGGCCTTTTCATCTCCTGGTGATGATGGCTTACCTCTAAGGGACCCCTTTATCAATGTTAGAATGAATACTCAATATGGTGAACCCCCTATTATTGCACCGGAAAACTCTAATGTATTGTCTGATGGTCAAATTAATGACACTATGCTGAGAATGGAGAGGAAACGCAAGAGTGAAGAAGTCAGAATGGCTAAAGAAGTAGAAGCTCATGAAGTTCGAATTCGGAAGGAGCTGGAGAAACAAGATATTCTAAGGAGAAAGAATGAGGAAAGAATGAAGAAAGAAATGGAAAGGCAAGACCGTGAAAGGCGAAAGGAGGAAGAGAGGTTGATGCGTGAGAGGCAGCGAGAGGAAGAGAGATCAAAGAAGGAGCAAAAACGTGAAATCGAACGAAGGGAGAAGTTTTTGCAAAAAGAACATATAAGAGCTGAGAAAAGGAGGCAAAAGGAAGAGCTCCGAAAAGAGAGAGAGGAAGTGAGACGCAAAGCTGCACTTGAGAAGGCTACTGCAAGAAGGCTTCTTAATAAATCTATGGAACTTTATGAGGATGAGCAACTAGAGCTAATGGAGTTGGCTGCTGCAAGCAAGGGATTATCCTCAATAATTAGTATTGATCCTGACACCAACCTTGATGAATTCAGAGATGATCTGACGGCATTTCCACCGAAGTCTGTGCTATTGAAAAAACCATTTGCAGTTCATCCGTGGATCGATTCAGAGGAGAACATCGGTAACTTTCTCATGGTTTGGAGATTTTTGATTACATTTGCAGATGTTCTTGAGTTATGGCCTTTTACTGTGGATGAGTTTGTTCAAGCTTTTCATGACTATGATTCAAGGTTGTTAGGAGAGATTCATGTTGCTCTCTTGAGGTTGATTATAAAGGATATAGAAGATGTTGCAAGGACACCTTCTACTGGACTAGGTCTCAATCAAAATGGTGCTGCTAATCCTGGAGGTGGACATCCACAGATTGTTGAAGGAGCATATGCATGGGGATTTGATATAAGGAACTGGCAGAAGCACTTAAATCTGCTAACATGGCCTGAAATTTTTCGCCAACTAGCACTCTCCGCTGGATTTGGGCCACAGTTGAAGAAAAGGAGTATCTCATGGTCATACTTGCCTGATAATGATGAGGGTAAAGGTTGTCGTGATGTAATTTCAACTCTCCGCAATGGTTCAGCAGCTGAAAATGCATTTGCAATAATGCAAGAAAAGGGCTTATTAGCTCCACGGAGATCCAGACATCGGTTGACGCCAGGAACTGTAAAGTTTGCAGCTTTTCATGTCCTTTCTCTTGAGGGAAACAAGGGATTGACGGTGTTAGAACTTGCTGACAAGATTCAGAAATCTGGCCTTCGAGACCTGACAACAAGCAAGACTCCAGAAGCTTCAATTTCAGTTGCTCTGACGAGAGATACTAAGCTTTTTGAAAGAATTGCTCCTTCAACATATCGTGTACGATCTGCTTACAGAAAGGACCCTATTGATGCTGAGGCCATACTTTCGGCAGCGAGGAAGAAGGTTCAGATATTTGAAAATGGGATTTTAGCTGCAGAAGATGTCGATGAGGTTGAAAGAGATGATGCCGAAGAGGTTGAGAGAGATGAAGACTCTGATTGTGATGACGTTGACGAGGACCCTGAAGTTGACGACTTAGCTACTCCAGCCATAGTGAAGAAATCCCCTGATCAGTTTAATGAAGTGACTCCTTTTTCAGAGAATGGACAGGAAAATTTATGCATTGATGTTGCACCAACTGTGCAAAATGAGTTTGATAAGGATGTTTCATCTTTCCCCGTTACTGCTTCCAAAGAGGCAGACGGTCCAAGTGCTTCCTCCAAGCAGTGTGTTTCTGGTGTAGAAATTAGTACTAGCAATCTTGATCAAGAAAATATGGAGATTGATGAGAGCAAGGCAGGTGAGTCATGGGTTCAAGGGCTTACAGAAGGGGATTATTCTGATCTCAGTGTCGAAGAGCGTCTTAGTTCTCTCGTTTCCTTAATTGGTATTGCAAATGAAGGAAACTCTATTCGTGTTGTTCTTGAGGATCGCTTAGAAGCAGCAAATGCTCTTAAGAAGCAAATGTGGGCAGAGGCACAGCTTGACAAAAGTCGCCTAAAAGAAGAGAATGTGAGTAAAGTTGATATTCCATCTTTCATGGGAGGCAAAGCTGAAGCCCATGTTCCCGGAGTGGAAGACGGCCAAAGTCCTCTACTGGATGTTTATAATAGAATCAATGAGGAAGGAGCTGCAGAAACTCAAAATTCGAATCATGGTTCACAAGTACTTCTGAATGGTGTGCCTGTTGAAAGGGCCATGGTACCTCAAGATACTTCCATGGGTCCTGAAAACATCTTGAATCAGCAATTGGCTTATGCATCAAAAAAGTCACGTTCACAGTTGAAATCATATATTGCCCACAGAGCAGAGGAGATGTATGCTTACAGGTCCCTCCCTCTTGGCCAAGATCGCAGGCATAATCGATACTGGCAATTTGTTGCATCTGCATCGAGCAATGATCCTGGTTCAGGCAGAATCTTTATTGAACTAAACAATGGAAATTGGAGGCTTATTGACACCGAGGAGGCATTTGACACTCTTTTGATGTCACTGGATACACGTGGGATTAGAGAATCCCATTTACGCTTAATGTTGCAAAAGATTGAGGCATCCTTCAAGGAAAATGTTCGTAGAAATTTGCATCCTTCAAGTAGAAATCATGTTAAAAAAGAAGCTGATGAAATGGATTCTAGTCCCGACTACCCTTCGGGTTTCGACAGCCCTGGAAGTACAGTTTCTGCTTTAAATACTGACGTTGGGGAGACGTCCTCTTCTTTCAGAATTGAGCTTAACCGAAACGAAAATGAGAAAAGGGCTGCCTTGAGTAGGTATCAAGATTTTCAGAAGTGGATGTGGAGAGAATGCTTCAGTACATCTGCATTATGTGCCTCGAAATATGGGAAAAAGAGGTGCAGACAGCTCTTTGATTTTTGCGATTTCTGCCTCAGTTGTTACCACTTTGAAGACTCCCACTGCAGTTTCTGCCATCAGACGTTTGGTGCTACCTATGAAAATCTTGACTTTTCTGAACATGTAATACAATGTAAAGAGAGAAGGAACTTGGAAACTTGCGATATCCATGTTCCTGGTACTTCTGTTCCTTTGGCCAGCAAATTGCTCAAGGCCTTCATGGCTCTTGTTGAGGTATCTGTTCCACCCGAAGCCCTTCAATCCTTTTGGACAGAAGACAGTCGAAAGACGTGGGGAGCAAAACTGAATGCGTCATCATCGGTTGAAGAGCTTCTGCAGATGTTAACTGTGTTTGAGACTGCGATGAAACGAGACTTTGTGTCATCAAACTTTGCAGCGACTGATGAATTACTGGGCTCCAGCAAACAGTCAGTGATTGCTAATCGTGATTATCTAGACACCAAATCAGTTTCTGTACTTCCTTGGATACCACATACCACGGCAGCTGTGGCTCTGAGGGTTTATGAGATGGACTCAGCAATTACATATATACCGAATGAGAAACCTGAGCCCAATGGCGACAAGGAAGTCGGAGAACATATTAAGATTCCTTTGAGATTCACCCCAATGAGAAATGATAGGGAGATCGAACCAACAGAGACAGACCACAACGAACAAAGTACTCACCTGAAAAGTGCACGAAACAGCCTCAAACGTGGTAGAGGAGGTCGTGAGCAAGGACGTGGTAAAAAGTCCAAATCTGGTGGTAGTCGGCGAAAAGCTAGAGGTAATGAGAACGAGAATATGAGTCAAGGATTAGGACCTGTGGGGCGAAGAACGCAGGGACAAGGGAGTGGACGGGGCCGACGTACTGTTAGAAAGAGGAGAATGAAGAACAGGGCTGTCGAGGGGACACTTATGGGTCATGTGACTGACCTACGCAGTAGCCCTGACAGTGGAGGGGAATCACCCAGAAACTTGGCTGGGGAATGGGATGATGAAAATATCAATATGATCCATATGAAAGGTGATGAACAACGAGAGGAGTATGAGCAAGCAGAGGCACTGGAATCTGATGACGAAGAACAAGCAGTGGGATATGAACAAGGGAACTGGGAGATTGGATTTGATGGGACCTCCAGCGGTTGGAACAGTGGTCTACGAGAACCTAGTGATGAGGAGATGGATGCTTCTGAAGATGATAATAATGGCATTGAGGAAGCAAGAGAAGAAGATTCGGAAGGGGATGTAGATTTGAGTGATGCTTCAGATGAAGTCCCAAACAGGATTATAAATGATGGCGGCACTGATTCAGCTTCAGACGATGATGATTACAGTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0000976 GO:0000977 GO:0000981 GO:0001012 GO:0001067 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1733

Amino Acids

194.67

Weight (kDa)

5.21

Isoelectric Point (pI)

51.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Homeodomain	PF00046	28 - 84	8e-17	Homeodomain
DDT	PF02791	535 - 590	1.1e-19	DDT domain
HARE-HTH	PF05066	716 - 784	7.4e-19	HB1, ASXL, restriction endonuclease HTH domain
WHIM1	PF15612	934 - 978	3.5e-06	WSTF, HB1, Itc1p, MBD9 motif 1
WSD	PF15613	1111 - 1184	1.3e-21	Williams-Beuren syndrome DDT (WSD), D-TOX E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28420
fragaria_vesca	FvH4_3g08620
malus_domestica	MD05G1310200.v1.1 MD10G1289100.v1.1
prunus_persica	Prupe.4G052600_v2.0.a1
pyrus_communis	pycom05g28660 pycom10g24170
rosa_chinensis	RchiOBHm_Chr5g0009221
rosa_laevigata	RLG00000031635 RLG00000031641
rosa_multiflora	Rmu_sc0006233.1_g000030
rosa_roxburghii	Rroxscaffold_1G00066840 Rroxscaffold_1G00067540
rosa_rugosa	Rorug04G0441600
rosa_samantha	Rh5AG071400 Rh5BG067900 Rh5CG080200 Rh5DG067900
rosa_wichuraiana	Rw5G006670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1763, 3114, 5150
AarI	CACCTGC	1 cut(s)	2795
AasI	GACNNNNNNGTC	1 cut(s)	4445
AatII	GACGTC	1 cut(s)	3716
Acc36I	ACCTGC	1 cut(s)	2795
Acc65I	GGTACC	1 cut(s)	3208
AccB1I	GGYRCC	2 cut(s)	613, 3208
AccB7I	CCANNNNNTGG	1 cut(s)	5004
AccI	GTMKAC	1 cut(s)	5017
AciI	CCGC	8 cut(s)	173, 320, 427, 640, 1956, 2056, 5001, 5160
AclWI	GGATC	6 cut(s)	1490, 1598, 2118, 2943, 3401, 4867
AcoI	YGGCCR	4 cut(s)	838, 3088, 3349, 4053
AcsI	RAATTY	6 cut(s)	1101, 1517, 1932, 2620, 3151, 3583
AcuI	CTGAAG	7 cut(s)	260, 2550, 3081, 3799, 5073, 5109, 5160
AcvI	CACGTG	1 cut(s)	3509
AcyI	GRCGYC	3 cut(s)	104, 2141, 3713
AflII	CTTAAG	1 cut(s)	2960
AflIII	ACRYGT	2 cut(s)	3506, 3979
AhdI	GACNNNNNGTC	2 cut(s)	382, 4237
AjiI	CACGTC	2 cut(s)	4149, 4601
AjnI	CCWGG	8 cut(s)	286, 440, 898, 1834, 2143, 3409, 3670, 4033
AjuI	GAANNNNNNNTTGG	2 cut(s)	3239, 3271
Alw21I	GWGCWC	2 cut(s)	1338, 4558
Alw26I	GTCTC	8 cut(s)	140, 1354, 1814, 2241, 3704, 4208, 4224, 4511
Alw44I	GTGCAC	1 cut(s)	4554
AlwI	GGATC	6 cut(s)	1490, 1598, 2118, 2943, 3401, 4867
AlwNI	CAGNNNCTG	6 cut(s)	2072, 2150, 3686, 3930, 4259, 4331
Ama87I	CYCGRG	2 cut(s)	38, 403
ApaLI	GTGCAC	1 cut(s)	4554
ApoI	RAATTY	6 cut(s)	1101, 1517, 1932, 2620, 3151, 3583
AseI	ATTAAT	1 cut(s)	1017
Asp700I	GAANNNNTTC	4 cut(s)	1935, 3573, 4113, 4188
Asp718I	GGTACC	1 cut(s)	3208
AspLEI	GCGC	1 cut(s)	93
AsuC2I	CCSGG	5 cut(s)	276, 282, 369, 599, 3075
AsuHPI	GGTGA	8 cut(s)	786, 914, 974, 2819, 4471, 4535, 4818, 4898
AsuII	TTCGAA	2 cut(s)	1099, 3155
AvaI	CYCGRG	2 cut(s)	38, 403
BaeGI	GKGCMC	2 cut(s)	616, 4558
BaeI	ACNNNNGTAYC	2 cut(s)	4029, 4062
BalI	TGGCCA	3 cut(s)	840, 3351, 4055
BanI	GGYRCC	2 cut(s)	613, 3208
BanII	GRGCYC	6 cut(s)	398, 410, 853, 1338, 4277, 4431
BbrPI	CACGTG	1 cut(s)	3509
BbsI	GAAGAC	6 cut(s)	107, 848, 2502, 3090, 3918, 4140
Bbv12I	GWGCWC	2 cut(s)	1338, 4558
BbvCI	CCTCAGC	1 cut(s)	2379
BceAI	ACGGC	4 cut(s)	91, 1551, 3103, 4374
BcgI	CGANNNNNNTGC	6 cut(s)	342, 376, 1537, 1571, 3378, 3412
BciT130I	CCWGG	8 cut(s)	288, 442, 900, 1836, 2145, 3411, 3672, 4035
BciVI	GTATCC	2 cut(s)	3496, 4338
BclI	TGATCA	3 cut(s)	586, 2575, 2770
BcnI	CCSGG	5 cut(s)	276, 282, 369, 599, 3075
BcoDI	GTCTC	8 cut(s)	140, 1354, 1814, 2241, 3704, 4208, 4224, 4511
BfmI	CTRYAG	4 cut(s)	2439, 3138, 3922, 4193
BfoI	RGCGCY	1 cut(s)	94
BfrI	CTTAAG	1 cut(s)	2960
BfuAI	ACCTGC	1 cut(s)	2795
BfuI	GTATCC	2 cut(s)	3496, 4338
BglI	GCCNNNNNGGC	1 cut(s)	4079
BglII	AGATCT	1 cut(s)	674
BmcAI	AGTACT	3 cut(s)	2758, 3174, 4540
BmeRI	GACNNNNNGTC	2 cut(s)	382, 4237
BmeT110I	CYCGRG	2 cut(s)	38, 403
BmgBI	CACGTC	2 cut(s)	4149, 4601
BmrI	ACTGGG	2 cut(s)	4280, 4981
BmuI	ACTGGG	2 cut(s)	4280, 4981
BoxI	GACNNNNGTC	1 cut(s)	2997
BpiI	GAAGAC	6 cut(s)	107, 848, 2502, 3090, 3918, 4140
BplI	GAGNNNNNCTC	4 cut(s)	3701, 3733, 4376, 4408
BpmI	CTGGAG	6 cut(s)	1136, 1857, 2253, 2535, 4261, 4981
Bpu10I	CCTNAGC	2 cut(s)	2379, 4425
Bpu14I	TTCGAA	2 cut(s)	1099, 3155
BpuEI	CTTGAG	8 cut(s)	1397, 1682, 1771, 2204, 2951, 3198, 3790, 4052
BpuMI	CCSGG	5 cut(s)	276, 282, 369, 599, 3075
Bsa29I	ATCGAT	2 cut(s)	1593, 3370
BsaAI	YACGTR	1 cut(s)	3509
BsaHI	GRCGYC	3 cut(s)	104, 2141, 3713
BsaI	GGTCTC	2 cut(s)	1814, 2241
BsaWI	WCCGGW	1 cut(s)	982
Bse118I	RCCGGY	1 cut(s)	292
Bse1I	ACTGG	8 cut(s)	1804, 1901, 3108, 3380, 3504, 4275, 4929, 4976
Bse3DI	GCAATG	4 cut(s)	436, 516, 2065, 3409
BseBI	CCWGG	8 cut(s)	288, 442, 900, 1836, 2145, 3411, 3672, 4035
BseCI	ATCGAT	2 cut(s)	1593, 3370
BseMI	GCAATG	4 cut(s)	436, 516, 2065, 3409
BseMII	CTCAG	8 cut(s)	1022, 1302, 2370, 2866, 3907, 4364, 4407, 4416
BseNI	ACTGG	8 cut(s)	1804, 1901, 3108, 3380, 3504, 4275, 4929, 4976
BseRI	GAGGAG	8 cut(s)	487, 1616, 3332, 3485, 4596, 4756, 4916, 5051
BseSI	GKGCMC	2 cut(s)	616, 4558
BseYI	CCCAGC	1 cut(s)	4841
BsgI	GTGCAG	2 cut(s)	1356, 3880
Bsh1285I	CGRYCG	1 cut(s)	205
BshNI	GGYRCC	2 cut(s)	613, 3208
BshVI	ATCGAT	2 cut(s)	1593, 3370
BsiEI	CGRYCG	1 cut(s)	205
BsiHKAI	GWGCWC	2 cut(s)	1338, 4558
BsiHKCI	CYCGRG	2 cut(s)	38, 403
BsiSI	CCGG	9 cut(s)	69, 275, 281, 293, 329, 368, 599, 983, 3075
BslFI	GGGAC	7 cut(s)	938, 3322, 3627, 4716, 4783, 5005, 5120
BsmAI	GTCTC	8 cut(s)	140, 1354, 1814, 2241, 3704, 4208, 4224, 4511
BsmBI	CGTCTC	3 cut(s)	140, 1354, 3704
BsmFI	GGGAC	7 cut(s)	938, 3322, 3627, 4716, 4783, 5005, 5120
BsmI	GAATGC	3 cut(s)	605, 3815, 4171
Bso31I	GGTCTC	2 cut(s)	1814, 2241
BsoBI	CYCGRG	2 cut(s)	38, 403
Bsp119I	TTCGAA	2 cut(s)	1099, 3155
Bsp1286I	GDGCHC	8 cut(s)	398, 410, 616, 853, 1338, 4277, 4431, 4558
Bsp19I	CCATGG	2 cut(s)	3204, 3225
BspACI	CCGC	8 cut(s)	173, 320, 427, 640, 1956, 2056, 5001, 5160
BspCNI	CTCAG	8 cut(s)	1023, 1303, 2371, 2865, 3906, 4365, 4406, 4417
BspDI	ATCGAT	2 cut(s)	1593, 3370
BspHI	TCATGA	3 cut(s)	757, 1090, 1705
BspMAI	CTGCAG	4 cut(s)	2443, 3142, 3926, 4197
BspMI	ACCTGC	1 cut(s)	2795
BspPI	GGATC	6 cut(s)	1490, 1598, 2118, 2943, 3401, 4867
BspQI	GCTCTTC	3 cut(s)	1326, 2856, 4179
BspT104I	TTCGAA	2 cut(s)	1099, 3155
BspT107I	GGYRCC	2 cut(s)	613, 3208
BspTI	CTTAAG	1 cut(s)	2960
BspTNI	GGTCTC	2 cut(s)	1814, 2241
BsrDI	GCAATG	4 cut(s)	436, 516, 2065, 3409
BsrFI	RCCGGY	1 cut(s)	292
BsrI	ACTGG	8 cut(s)	1804, 1901, 3108, 3380, 3504, 4275, 4929, 4976
BssAI	RCCGGY	1 cut(s)	292
BssNI	GRCGYC	3 cut(s)	104, 2141, 3713
BssT1I	CCWWGG	4 cut(s)	467, 3204, 3225, 4340
Bst2UI	CCWGG	8 cut(s)	288, 442, 900, 1836, 2145, 3411, 3672, 4035
Bst6I	CTCTTC	8 cut(s)	1197, 1230, 1326, 2472, 2856, 3006, 3724, 4179
BstACI	GRCGYC	3 cut(s)	104, 2141, 3713
BstAFI	CTTAAG	1 cut(s)	2960
BstAPI	GCANNNNNTGC	3 cut(s)	2954, 3833, 3930
BstBAI	YACGTR	1 cut(s)	3509
BstBI	TTCGAA	2 cut(s)	1099, 3155
BstC8I	GCNNGC	6 cut(s)	83, 879, 1465, 3362, 4057, 4914
BstDSI	CCRYGG	5 cut(s)	1586, 2117, 3204, 3225, 4356
BstENI	CCTNNNNNAGG	3 cut(s)	128, 3414, 3772
BstH2I	RGCGCY	1 cut(s)	94
BstHHI	GCGC	1 cut(s)	93
BstMAI	GTCTC	8 cut(s)	140, 1354, 1814, 2241, 3704, 4208, 4224, 4511
BstMCI	CGRYCG	1 cut(s)	205
BstNI	CCWGG	8 cut(s)	288, 442, 900, 1836, 2145, 3411, 3672, 4035
BstNSI	RCATGY	1 cut(s)	3983
BstPAI	GACNNNNGTC	1 cut(s)	2997
BstSFI	CTRYAG	4 cut(s)	2439, 3138, 3922, 4193
BstSLI	GKGCMC	2 cut(s)	616, 4558
BstV2I	GAAGAC	6 cut(s)	107, 848, 2502, 3090, 3918, 4140
BstX2I	RGATCY	2 cut(s)	674, 2123
BstXI	CCANNNNNNTGG	2 cut(s)	4034, 4837
BstYI	RGATCY	2 cut(s)	674, 2123
Bsu15I	ATCGAT	2 cut(s)	1593, 3370
BsuI	GTATCC	2 cut(s)	3496, 4338
BsuTUI	ATCGAT	2 cut(s)	1593, 3370
BtgI	CCRYGG	5 cut(s)	1586, 2117, 3204, 3225, 4356
BtgZI	GCGATG	1 cut(s)	4235
BtrI	CACGTC	2 cut(s)	4149, 4601
BtsI	GCAGTG	4 cut(s)	888, 2738, 3919, 4956
BveI	ACCTGC	1 cut(s)	2795
Cac8I	GCNNGC	6 cut(s)	83, 879, 1465, 3362, 4057, 4914
CaiI	CAGNNNCTG	6 cut(s)	2072, 2150, 3686, 3930, 4259, 4331
CciI	TCATGA	3 cut(s)	757, 1090, 1705
CfoI	GCGC	1 cut(s)	93
Cfr10I	RCCGGY	1 cut(s)	292
ClaI	ATCGAT	2 cut(s)	1593, 3370
CseI	GACGC	5 cut(s)	112, 1371, 2149, 2855, 4158
CspCI	CAANNNNNGTGG	2 cut(s)	4603, 4638
DraI	TTTAAA	1 cut(s)	3693
DrdI	GACNNNNNNGTC	1 cut(s)	4445
DriI	GACNNNNNGTC	2 cut(s)	382, 4237
DseDI	GACNNNNNNGTC	1 cut(s)	4445
EaeI	YGGCCR	4 cut(s)	838, 3088, 3349, 4053
Eam1104I	CTCTTC	8 cut(s)	1197, 1230, 1326, 2472, 2856, 3006, 3724, 4179
Eam1105I	GACNNNNNGTC	2 cut(s)	382, 4237
EarI	CTCTTC	8 cut(s)	1197, 1230, 1326, 2472, 2856, 3006, 3724, 4179
EciI	GGCGGA	1 cut(s)	188
Ecl136II	GAGCTC	1 cut(s)	1336
Eco130I	CCWWGG	4 cut(s)	467, 3204, 3225, 4340
Eco147I	AGGCCT	2 cut(s)	134, 4073
Eco24I	GRGCYC	6 cut(s)	398, 410, 853, 1338, 4277, 4431
Eco31I	GGTCTC	2 cut(s)	1814, 2241
Eco32I	GATATC	1 cut(s)	4024
Eco53kI	GAGCTC	1 cut(s)	1336
Eco57I	CTGAAG	7 cut(s)	260, 2550, 3081, 3799, 5073, 5109, 5160
Eco72I	CACGTG	1 cut(s)	3509
Eco88I	CYCGRG	2 cut(s)	38, 403
EcoICRI	GAGCTC	1 cut(s)	1336
EcoNI	CCTNNNNNAGG	3 cut(s)	128, 3414, 3772
EcoO109I	RGGNCCY	7 cut(s)	925, 2365, 2524, 3230, 3336, 4679, 4992
EcoRI	GAATTC	2 cut(s)	1101, 1517
EcoRII	CCWGG	8 cut(s)	286, 440, 898, 1834, 2143, 3409, 3670, 4033
EcoRV	GATATC	1 cut(s)	4024
EcoT14I	CCWWGG	4 cut(s)	467, 3204, 3225, 4340
EcoT22I	ATGCAT	5 cut(s)	607, 1876, 2083, 2630, 3268
EcoT38I	GRGCYC	6 cut(s)	398, 410, 853, 1338, 4277, 4431
ErhI	CCWWGG	4 cut(s)	467, 3204, 3225, 4340
Esp3I	CGTCTC	3 cut(s)	140, 1354, 3704
FalI	AAGNNNNNCTT	6 cut(s)	1359, 1391, 1777, 1809, 2853, 2885
FaqI	GGGAC	7 cut(s)	938, 3322, 3627, 4716, 4783, 5005, 5120
FauNDI	CATATG	2 cut(s)	1870, 4878
FbaI	TGATCA	3 cut(s)	586, 2575, 2770
FblI	GTMKAC	1 cut(s)	5017
FriOI	GRGCYC	6 cut(s)	398, 410, 853, 1338, 4277, 4431
GlaI	GCGC	1 cut(s)	92
GsaI	CCCAGC	1 cut(s)	4845
GsuI	CTGGAG	6 cut(s)	1136, 1857, 2253, 2535, 4261, 4981
HaeII	RGCGCY	1 cut(s)	94
HapII	CCGG	9 cut(s)	69, 275, 281, 293, 329, 368, 599, 983, 3075
HgaI	GACGC	5 cut(s)	112, 1371, 2149, 2855, 4158
HhaI	GCGC	1 cut(s)	93
Hin1I	GRCGYC	3 cut(s)	104, 2141, 3713
Hin6I	GCGC	1 cut(s)	91
HinP1I	GCGC	1 cut(s)	91
HincII	GTYRAC	4 cut(s)	2139, 2518, 2536, 4203
HindII	GTYRAC	4 cut(s)	2139, 2518, 2536, 4203
HindIII	AAGCTT	3 cut(s)	1698, 2274, 2309
HpaI	GTTAAC	1 cut(s)	4203
HpaII	CCGG	9 cut(s)	69, 275, 281, 293, 329, 368, 599, 983, 3075
HphI	GGTGA	8 cut(s)	786, 914, 974, 2819, 4471, 4535, 4818, 4898
Hpy99I	CGWCG	1 cut(s)	4728
Hsp92I	GRCGYC	3 cut(s)	104, 2141, 3713
HspAI	GCGC	1 cut(s)	91
KflI	GGGWCCC	1 cut(s)	925
KpnI	GGTACC	1 cut(s)	3212
Ksp22I	TGATCA	3 cut(s)	586, 2575, 2770
KspAI	GTTAAC	1 cut(s)	4203
LguI	GCTCTTC	3 cut(s)	1326, 2856, 4179
MfeI	CAATTG	5 cut(s)	339, 536, 590, 768, 3254
MflI	RGATCY	2 cut(s)	674, 2123
MhlI	GDGCHC	8 cut(s)	398, 410, 616, 853, 1338, 4277, 4431, 4558
MlsI	TGGCCA	3 cut(s)	840, 3351, 4055
MluNI	TGGCCA	3 cut(s)	840, 3351, 4055
MlyI	GAGTC	9 cut(s)	170, 494, 2260, 2492, 2587, 2819, 3908, 4385, 4675
MmeI	TCCRAC	6 cut(s)	123, 162, 342, 803, 4429, 4985
Mox20I	TGGCCA	3 cut(s)	840, 3351, 4055
Mph1103I	ATGCAT	5 cut(s)	607, 1876, 2083, 2630, 3268
MroXI	GAANNNNTTC	4 cut(s)	1935, 3573, 4113, 4188
MscI	TGGCCA	3 cut(s)	840, 3351, 4055
MslI	CAYNNNNRTG	4 cut(s)	756, 1710, 1873, 4484
Msp20I	TGGCCA	3 cut(s)	840, 3351, 4055
MspA1I	CMGCKG	4 cut(s)	1958, 2072, 4364, 5001
MspCI	CTTAAG	1 cut(s)	2960
MspI	CCGG	9 cut(s)	69, 275, 281, 293, 329, 368, 599, 983, 3075
MunI	CAATTG	5 cut(s)	339, 536, 590, 768, 3254
Mva1269I	GAATGC	3 cut(s)	605, 3815, 4171
MvaI	CCWGG	8 cut(s)	288, 442, 900, 1836, 2145, 3411, 3672, 4035
NciI	CCSGG	5 cut(s)	276, 282, 369, 599, 3075
NcoI	CCATGG	2 cut(s)	3204, 3225
NdeI	CATATG	2 cut(s)	1870, 4878
NmuCI	GTSAC	8 cut(s)	227, 374, 486, 861, 2590, 3276, 3495, 4786
NsiI	ATGCAT	5 cut(s)	607, 1876, 2083, 2630, 3268
NspI	RCATGY	1 cut(s)	3983
NspV	TTCGAA	2 cut(s)	1099, 3155
PaeR7I	CTCGAG	1 cut(s)	403
PagI	TCATGA	3 cut(s)	757, 1090, 1705
PaqCI	CACCTGC	1 cut(s)	2795
PceI	AGGCCT	2 cut(s)	134, 4073
PciI	ACATGT	1 cut(s)	3979
PciSI	GCTCTTC	3 cut(s)	1326, 2856, 4179
PcsI	WCGNNNNNNNCGW	1 cut(s)	4723
PctI	GAATGC	3 cut(s)	605, 3815, 4171
PdmI	GAANNNNTTC	4 cut(s)	1935, 3573, 4113, 4188
PflFI	GACNNNGTC	1 cut(s)	1806
PflMI	CCANNNNNTGG	1 cut(s)	5004
PfoI	TCCNGGA	4 cut(s)	286, 440, 1834, 3073
Ple19I	CGATCG	1 cut(s)	205
PleI	GAGTC	9 cut(s)	169, 493, 2260, 2492, 2587, 2818, 3908, 4385, 4674
PmaCI	CACGTG	1 cut(s)	3509
PmlI	CACGTG	1 cut(s)	3509
PpsI	GAGTC	9 cut(s)	169, 493, 2260, 2492, 2587, 2818, 3908, 4385, 4674
Ppu21I	YACGTR	1 cut(s)	3509
PpuMI	RGGWCCY	7 cut(s)	925, 2365, 2524, 3230, 3336, 4679, 4992
PscI	ACATGT	1 cut(s)	3979
PshAI	GACNNNNGTC	1 cut(s)	2997
PshBI	ATTAAT	1 cut(s)	1017
PsiI	TTATAA	3 cut(s)	1763, 3114, 5150
Psp124BI	GAGCTC	1 cut(s)	1338
Psp5II	RGGWCCY	7 cut(s)	925, 2365, 2524, 3230, 3336, 4679, 4992
Psp6I	CCWGG	8 cut(s)	286, 440, 898, 1834, 2143, 3409, 3670, 4033
PspCI	CACGTG	1 cut(s)	3509
PspFI	CCCAGC	1 cut(s)	4841
PspGI	CCWGG	8 cut(s)	286, 440, 898, 1834, 2143, 3409, 3670, 4033
PspPPI	RGGWCCY	7 cut(s)	925, 2365, 2524, 3230, 3336, 4679, 4992
PspXI	VCTCGAGB	1 cut(s)	403
PsrI	GAACNNNNNNTAC	2 cut(s)	3969, 4001
PstI	CTGCAG	4 cut(s)	2443, 3142, 3926, 4197
PstNI	CAGNNNCTG	6 cut(s)	2072, 2150, 3686, 3930, 4259, 4331
PsuI	RGATCY	2 cut(s)	674, 2123
PsyI	GACNNNGTC	1 cut(s)	1806
PvuI	CGATCG	1 cut(s)	205
PvuII	CAGCTG	2 cut(s)	2072, 4364
RseI	CAYNNNNRTG	4 cut(s)	756, 1710, 1873, 4484
SacI	GAGCTC	1 cut(s)	1338
SapI	GCTCTTC	3 cut(s)	1326, 2856, 4179
ScaI	AGTACT	3 cut(s)	2758, 3174, 4540
SchI	GAGTC	9 cut(s)	170, 494, 2260, 2492, 2587, 2819, 3908, 4385, 4675
SduI	GDGCHC	8 cut(s)	398, 410, 616, 853, 1338, 4277, 4431, 4558
SfcI	CTRYAG	4 cut(s)	2439, 3138, 3922, 4193
Sfr274I	CTCGAG	1 cut(s)	403
SfuI	TTCGAA	2 cut(s)	1099, 3155
SlaI	CTCGAG	1 cut(s)	403
SmiMI	CAYNNNNRTG	4 cut(s)	756, 1710, 1873, 4484
SseBI	AGGCCT	2 cut(s)	134, 4073
SsiI	CCGC	8 cut(s)	173, 320, 427, 640, 1956, 2056, 5001, 5160
SstI	GAGCTC	1 cut(s)	1338
StuI	AGGCCT	2 cut(s)	134, 4073
StyI	CCWWGG	4 cut(s)	467, 3204, 3225, 4340
TatI	WGTACW	6 cut(s)	2756, 3172, 3677, 3818, 4333, 4538
TauI	GCSGC	2 cut(s)	643, 5163
TseFI	GTSAC	8 cut(s)	227, 374, 486, 861, 2590, 3276, 3495, 4786
Tsp45I	GTSAC	8 cut(s)	227, 374, 486, 861, 2590, 3276, 3495, 4786
TspGWI	ACGGA	3 cut(s)	518, 1575, 2134
Tth111I	GACNNNGTC	1 cut(s)	1806
Van91I	CCANNNNNTGG	1 cut(s)	5004
Vha464I	CTTAAG	1 cut(s)	2960
VneI	GTGCAC	1 cut(s)	4554
VspI	ATTAAT	1 cut(s)	1017
XagI	CCTNNNNNAGG	3 cut(s)	128, 3414, 3772
XapI	RAATTY	6 cut(s)	1101, 1517, 1932, 2620, 3151, 3583
XbaI	TCTAGA	1 cut(s)	4312
XceI	RCATGY	1 cut(s)	3983
XcmI	CCANNNNNNNNNTGG	3 cut(s)	523, 3941, 4363
XhoI	CTCGAG	1 cut(s)	403
XmiI	GTMKAC	1 cut(s)	5017
XmnI	GAANNNNTTC	4 cut(s)	1935, 3573, 4113, 4188
ZraI	GACGTC	1 cut(s)	3714
ZrmI	AGTACT	3 cut(s)	2758, 3174, 4540
Zsp2I	ATGCAT	5 cut(s)	607, 1876, 2083, 2630, 3268

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.