Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0011708

Physical Location & Seq

Genomic Coordinates Forward (+)

88320179 .. 88329270

Gene Structure 9092 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00066840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5211 bp

ATGGAGGGTTCGGAAGGAGATAATCAGAATCGGAACCATGAGAATAGCAATAGTAAATTGAACAATTCCGGCGAGGGAGGCAAGCCCAAGCGCCAGATGAAGACGCCGTTTCAGCTCGAAACCCTAGAGAAGGCCTATGCGTTGGAGACGTACCCATCGGAGTCGATTAGGGCGGACCTGTCGGAGAAATTGGGGCTTTCCGATCGGCAGTTGCAGATGTGGTTCTGTCACAGAAGGCTGAAGGATAAGAAGGAAGGAGGTTCGGGTTCGGGTCCGGGTCCAGGTCCGGGTCCAGGACCGGCGAAGAAGCAGAGGAAATCAGTGGCGGTGTTGCCGGAGCCTCCAATTGATGACTTGGCACTTGGGTCGGAGCCGGCGAGTGACTATGGGTCGGGTTCGGGCTCTGGCTCGAGCCCATTTGGTCATGCAGACCGCAATGTGGTGTCCAGGAGTGTTGTTGTTGAGGATATGCCAAGGAGGAGGCATTATGAGTCACAGCAGTCGATTACAGAGCTTAGAGCCATTGCTATTGTGGAAGCTCAATTGGGAGAGCCATTAAGGGAAGATGGCCCTGTGCTTGGGATAGAGTTTGATCAATTGCCCCCGGATGCATTCGGGGCACCTCTAGGTATACAGCTTGGTTTGAGATGCTTTGTACCCGACTTATATAGGGGTACTCCGAGATCTCTCCATGAATATCCATATCTTCAAGACCACCCCATTATTCGGCCTGATGTATATGGACAAGTTGCTCAATCTCATTTTCATGATTCAGCAATTGATGGTCCAACTGCAAGAGCTTCACCATTTGCAAGTGGAAATGAACAGTCGTCAAGGGTTCACGGTGGCCACAGTCGGGCTCGTCTTCTGTCACAACAGGATAGGCAAGCAGTGGCCTTTTCATCTCCTGGTGATGATGGCTTACCTCAAAGGGACCCCTTTATCAATGTTAGAATGAATACTCAATATGGTGAACCCCCTATTATTGCACCGGAAAACTCTAATGTATTGTCTGATGGTCAAATTAATGACACTATGCTGAGAATGGAGAGGAAACGCAAGAGTGAAGAAGTCAGAATGGCTAAAGAAGTAGAAGCTCATGAAGTTCGAATTCGGAAGGAGCTGGAGAAACAAGATATTCTGAGGAGAAAGAATGAGGAAAGAATGAGGAAAGAAATGGAAAGGCAAGACCGTGAAAGGCGAAAGGAGGAAGAGAGGTTGATGCGTGAGAGGCAGCGAGAGGAAGAGAGATCAAAGAAGGAGCAAAAACGTGAAATCGAACGAAGGGAGAAGTTTTTGCAAAAAGAACATATAAGAGCTGAGAAAAGGAGGCAAAAGGAAGAGCTCCGAAAAGAGAGAGAGGAAGTGAGACGCAAAGCTGCACTTGAGAAGGCTACTGCAAGAAGGCTTCTTAATAAATCTATGGAACTTTATGAGGATGAGCAACTAGAGCTAATGGAGTTGGCTGCTGCAAGCAAGGGATTATCCTCAATAATTAGTATTGATCCCGACACCAACCTTGATGAATTCAGAGATGATCTGATGGCATTTCCACCCAAGTCTGTGCTATTGAAAAAACCATTTGCAGTTCATCCGTGGATCAATTCAGAGGAGAACATTGGTAACTTTCTCATGGTTTGGAGATTTTTGATTACATTTGCAGATGTTCTCGAGTTATGGCCTTTTACTGTGGATGAGTTTGTTCAAGCTTTTCATGACTATGATTCAAGGTTGTTAGGAGAGATTCATGTTGCTCTCTTGAGGTTGATTATAAAGGATATAGAAGATGTTGCAAGGACACCTTCTACTGGACTAGGTCTCAATCAAAATGGTGCTGCTAATCCTGGAGGTGGACATCCACAGATTGTTGAAGGAGCATATGCGTGGGGATTTGACATAAGGAACTGGCAGAAGCACTTAAATCTGCTAACATGGCCTGAAATTTTTCGCCAACTAGCACTCTCTGCTGGATTTGGACCACAGTTGAAGAAAAGGAGTATCTCATGGTCGTACTTGCCTGATAATGATGAGGGTAAAGGTTGTCGTGATGTAATTTCAACTCTCCGCAATGGTTCAGCAGCTGAAAATGCATTTGCAATAATGCAAGAAAAGGGCTTATTAGCTCCCCGGAGATCCAGACATCGGTTGACACCGGGAACTGTAAAGTTTGCAGCTTTTCATGTCCTTTCTCTTGAGGGAAACAAGGGATTGACGGTGTTAGAACTTGCTGACAAGATTCAGAAATCTGGCCTTCGAGACCTGACAACAAGCAAGACTCCAGAGGCTTCAATTTCAGTTGCTCTGACAAGAGATACTAAGCTTTTTGAAAGAATTGCTCCTTCAACATATCGTGTACGATCTGCTTACAGAAAGGACCCTATTGATGCTGAGGCCATACTTTCTGCAGCTAGGAAGAAGGTTCAGATATTTGAAAATGGGATTTTAGCTGCAGAAGATGTCGATGAAGTTGAAAGAGATGATGCCGAAGAGGTTGAGAGAGATGAAGACTCTGATTGTGATGACGTTGATGAGGACCCTGAAGTTGACGACTTAGCTACTCCAGCCATAGTGAAGAAATCTCCTGATCAGTTTAATGAAGTGACTCCTTTTTCAGAGAATGGACAGGAAAATTTATGCATTGATGTTGCACCAACTGTGCAAAATGAGTTTGATAAGGATGTTTCATCTTTCCCCGTTACTGCTTCCAAAGAGGCAGACGGTCCAAGTGCTTCCTCCAAGCAGTGTGTTTCTGGTGTAGAAATTAGTACTAGCAATCTTGATCAAGAAAATATGGAGATTGATGAGAGCAAGGCAGGTGAGTCATGGGTTCAAGGGCTTACAGAAGGGGATTATTCTGATCTCAGTGTCGAAGAGCGTCTTAGTTCTCTCGTTTCCTTAATTGGTATTGCAAATGAAGGAAACTCTATTCGTGTTGTTCTTGAGGATCGCTTAGAAGCAGCAAATGCTCTTAAGAAGCAAATGTGGGCGGAGGCACAGCTTGACAAAAGTCGCCTAAAAGAAGAGAATGTGAGTAAATTTGATATTCCATCTTTGATGGGAGGCAAAGCTGAAGCACATGTTCCTGGAGTGGAAGACGGCCAAAGTCCTCTACTGGATGTTTATAATAGAATCAATGAGGAGGGAGCTGCAGAAACTCAAAATTCGAATCATGGTTCACAAGTACTTCTGAATGGTGTGCCTGTTGAAAGGGCCATGGTACCTCAAGATACTTCCATGGGTCCTGAAAACATCTTGAATCAGCAATTGGCTTATGCATCAAAAAAGTCACGTTCACAGTTGAAATCATATATTGCCCACAGAGCAGAGGAGATGTATGCTTACAGGTCCCTCCCTCTTGGCCAAGATCGCAGGCATAATCGATACTGGCAATTTGTTGCATCTGCATCGAGCAATGATCCTGGTTCAGGCAGAATCTTTATTGAACTAAACAATGGAAATTGGAGGCTTATTGACACCGAAGAGGCATTTGACACTCTTTTGATGTCACTGGATACACGTGGGATTAGAGAATCCCACTTACGCTTAATGTTGCAAAAGATTGAGGCATCCTTCAAGGAAAATGTTCGTAGAAATGTGCATCCTTCAAGTAGAAATCATGTTAAAAAAGAAGCTGATGAAATGGATTCTAGTCCCGACTACCCTTCGGGTTTCGACAGCCCTGGAAGTACAGTTTCTGCTTTAAATACTGACATTGGGGAGACGTCCTCTTCTTTCAGAATTGAGCTTAACCGAAATGAAAATGAGAAAAGGGCTGCCTTGAGTAGGTATCAAGATTTTCAGAAGTGGATGTGGAGAGAATGCTTTAGTACATCTGCATTATGTGCCTCGAAATATGGGAAAAAGAGGTGCAGACAGCTCTTTGATTTTTGCGATTTCTGCCTCAGTTGTTACCACTTTGAAGACTCCCACTGCAGTTTCTGCCATCAGACGTTTGGTGCTACCTATGAAAATCTTGACTTTTCTGAACATGTAATACAATGTAAAGAGAGAAGGAACTTGGAAACTTGCGATATCCATGGTCCTGGTACTGCTGTTCCTTTGGCCAGCAAATTGCTCAAGGCCTTCATGGCTCTTGTTGAGGTATCTGTTCCACCCGAAGCCCTTCAATCCTTTTGGACAGAAGACTGTCGAAAGATGTGGGGAGCAAAACTGAATGCGTCATCATCGGTTGAAGAGCTTCTGCAGATGTTAACTGTGTTTGAGACTGCGATGAAACGAGACTTTGTGTCATCAAACTTTGCAGCGACTGATGAATTACTGGGCTCCAGCAAACAGTCAGTGATTGCTAATCATGATTATCTAGACACCAAATCAGTTTCTGTACTTCCTTGGATACCACATACCACGGCAGCTGTGGCTCTGAGGGTTTATGAGATGGACTCAGCAATTACATATATACCGAATGAGAAACCTGAGCCCAATGGCGACAAGGAAGTCAGAGAACATATTAAGATTCCTTTGAGATTCACCCCAATGAGAAATGATAGGGAGATCGAACCAACAGAGACAGACCACAACGAACAAAGTACTCACCTGAAAAGTGCACGAAACAGCCTCAAACGTGGTAGAGGAGGTCGTGAGCAAGGACGTGGTAAAAAGTCCAAATCTGGTGGTAGTCGGCGAAAAGCTAGAGGTAATGAGAACGAGAATATGAGTCAAGGATTAGGACCTGTGGGGCGAAGAACGCAGGGACAAGGGAGTGGACGGGGCCGACGTACTGTTAGAAAGAGGAGAATGAAGAACAGGGCTGTCGAGGGGACACTTATGGGTCATGTGACTGACCTACGCAGTAGCCCCGAAAGTGGAGGGGAATCACCAAGAAACTTGGCTGGGGAATGGGATGATGAAAATATCAATATGATCCATATGAAAGGTGATGAACAACGAGAGGAGTATGAGCAAGCAGAGGCACTGGAATCTGACGACGAAGAACAAGCAGTGGGATATGAACAAGGGAACTGGGAAATTGGATTTGATGGGACCTCCAGCGCTTGGAACAGCGGTCTACGAGAACCCAGTGATGAGGAGATGGATGCTTCTGAAGATGATAATAATGGCATTGAGGAAGCGAGAGAAGAAGATTCGGAAGGGGATGTAGATATGAGTGATGCTTCAGATGAAGTCCCAAACAGGATTATAAATGATGATGGCACTGATTCAGCTTCAGACGATGATGATTACAGTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0000976 GO:0000977 GO:0000981 GO:0001012 GO:0001067 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1736

Amino Acids

195.17

Weight (kDa)

5.2

Isoelectric Point (pI)

51.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Homeodomain	PF00046	28 - 84	1.4e-16	Homeodomain
DDT	PF02791	538 - 593	1.1e-19	DDT domain
HARE-HTH	PF05066	719 - 787	7.4e-19	HB1, ASXL, restriction endonuclease HTH domain
WHIM1	PF15612	937 - 981	3.5e-06	WSTF, HB1, Itc1p, MBD9 motif 1
WSD	PF15613	1114 - 1187	1.3e-21	Williams-Beuren syndrome DDT (WSD), D-TOX E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28420
fragaria_vesca	FvH4_3g08620
malus_domestica	MD05G1310200.v1.1 MD10G1289100.v1.1
prunus_persica	Prupe.4G052600_v2.0.a1
pyrus_communis	pycom05g28660 pycom10g24170
rosa_chinensis	RchiOBHm_Chr5g0009221
rosa_laevigata	RLG00000031635 RLG00000031641
rosa_multiflora	Rmu_sc0006233.1_g000030
rosa_roxburghii	Rroxscaffold_1G00066840 Rroxscaffold_1G00067540
rosa_rugosa	Rorug04G0441600
rosa_samantha	Rh5AG071400 Rh5BG067900 Rh5CG080200 Rh5DG067900
rosa_wichuraiana	Rw5G006670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1772, 3123, 5159
AarI	CACCTGC	1 cut(s)	2804
AasI	GACNNNNNNGTC	1 cut(s)	4454
AatII	GACGTC	1 cut(s)	3725
Acc36I	ACCTGC	1 cut(s)	2804
Acc65I	GGTACC	1 cut(s)	3217
AccB1I	GGYRCC	2 cut(s)	619, 3217
AccB7I	CCANNNNNTGG	1 cut(s)	5013
AccI	GTMKAC	2 cut(s)	631, 5026
AciI	CCGC	6 cut(s)	173, 326, 433, 2065, 2987, 5022
AclWI	GGATC	6 cut(s)	1499, 1607, 2127, 2952, 3410, 4876
AcoI	YGGCCR	4 cut(s)	847, 3097, 3358, 4062
AcsI	RAATTY	6 cut(s)	1110, 1526, 1941, 2629, 3035, 3160
AcuI	CTGAAG	6 cut(s)	260, 2559, 3090, 5082, 5118, 5169
AcvI	CACGTG	1 cut(s)	3518
AcyI	GRCGYC	2 cut(s)	104, 3722
AfeI	AGCGCT	1 cut(s)	5011
AflII	CTTAAG	1 cut(s)	2969
AflIII	ACRYGT	3 cut(s)	3076, 3515, 3988
AhdI	GACNNNNNGTC	2 cut(s)	388, 4246
AjiI	CACGTC	1 cut(s)	4610
AjnI	CCWGG	9 cut(s)	280, 292, 446, 907, 1843, 3082, 3418, 3679, 4042
AjuI	GAANNNNNNNTTGG	2 cut(s)	3248, 3280
Alw21I	GWGCWC	2 cut(s)	1347, 4567
Alw26I	GTCTC	8 cut(s)	140, 1363, 1823, 2250, 3713, 4217, 4233, 4520
Alw44I	GTGCAC	1 cut(s)	4563
AlwI	GGATC	6 cut(s)	1499, 1607, 2127, 2952, 3410, 4876
AlwNI	CAGNNNCTG	5 cut(s)	2081, 3695, 3939, 4268, 4340
Ama87I	CYCGRG	2 cut(s)	409, 1670
Aor51HI	AGCGCT	1 cut(s)	5011
ApaLI	GTGCAC	1 cut(s)	4563
ApoI	RAATTY	6 cut(s)	1110, 1526, 1941, 2629, 3035, 3160
AseI	ATTAAT	1 cut(s)	1026
Asp700I	GAANNNNTTC	4 cut(s)	1944, 3582, 4122, 4197
Asp718I	GGTACC	1 cut(s)	3217
AspLEI	GCGC	2 cut(s)	93, 5012
AsuC2I	CCSGG	5 cut(s)	276, 288, 605, 2128, 2154
AsuHPI	GGTGA	8 cut(s)	795, 923, 983, 2828, 4480, 4544, 4827, 4907
AsuII	TTCGAA	2 cut(s)	1108, 3164
AvaI	CYCGRG	2 cut(s)	409, 1670
BaeGI	GKGCMC	2 cut(s)	622, 4567
BaeI	ACNNNNGTAYC	2 cut(s)	4038, 4071
BalI	TGGCCA	3 cut(s)	849, 3360, 4064
BanI	GGYRCC	2 cut(s)	619, 3217
BanII	GRGCYC	6 cut(s)	404, 416, 862, 1347, 4286, 4440
BbrPI	CACGTG	1 cut(s)	3518
BbsI	GAAGAC	6 cut(s)	107, 857, 2511, 3099, 3927, 4149
Bbv12I	GWGCWC	2 cut(s)	1347, 4567
BbvCI	CCTCAGC	1 cut(s)	2388
BceAI	ACGGC	3 cut(s)	91, 3112, 4383
BcgI	CGANNNNNNTGC	4 cut(s)	348, 382, 3387, 3421
BciT130I	CCWGG	9 cut(s)	282, 294, 448, 909, 1845, 3084, 3420, 3681, 4044
BciVI	GTATCC	2 cut(s)	3505, 4347
BclI	TGATCA	3 cut(s)	592, 2584, 2779
BcnI	CCSGG	5 cut(s)	276, 288, 605, 2128, 2154
BcoDI	GTCTC	8 cut(s)	140, 1363, 1823, 2250, 3713, 4217, 4233, 4520
BfmI	CTRYAG	5 cut(s)	2403, 2448, 3147, 3931, 4202
BfoI	RGCGCY	2 cut(s)	94, 5013
BfrI	CTTAAG	1 cut(s)	2969
BfuAI	ACCTGC	1 cut(s)	2804
BfuI	GTATCC	2 cut(s)	3505, 4347
BglI	GCCNNNNNGGC	1 cut(s)	4088
BglII	AGATCT	1 cut(s)	683
BmcAI	AGTACT	3 cut(s)	2767, 3183, 4549
BmeRI	GACNNNNNGTC	2 cut(s)	388, 4246
BmeT110I	CYCGRG	2 cut(s)	409, 1670
BmgBI	CACGTC	1 cut(s)	4610
BmrI	ACTGGG	3 cut(s)	4289, 4990, 5031
BmuI	ACTGGG	3 cut(s)	4289, 4990, 5031
BoxI	GACNNNNGTC	1 cut(s)	3006
BpiI	GAAGAC	6 cut(s)	107, 857, 2511, 3099, 3927, 4149
BplI	GAGNNNNNCTC	4 cut(s)	3710, 3742, 4385, 4417
BpmI	CTGGAG	7 cut(s)	1145, 1866, 2262, 2544, 3105, 4270, 4990
Bpu10I	CCTNAGC	2 cut(s)	2388, 4434
Bpu14I	TTCGAA	2 cut(s)	1108, 3164
BpuEI	CTTGAG	7 cut(s)	1406, 1780, 2213, 2960, 3207, 3799, 4061
BpuMI	CCSGG	5 cut(s)	276, 288, 605, 2128, 2154
Bsa29I	ATCGAT	1 cut(s)	3379
BsaAI	YACGTR	1 cut(s)	3518
BsaHI	GRCGYC	2 cut(s)	104, 3722
BsaI	GGTCTC	2 cut(s)	1823, 2250
BsaWI	WCCGGW	1 cut(s)	991
Bse118I	RCCGGY	2 cut(s)	298, 373
Bse1I	ACTGG	9 cut(s)	1813, 1910, 3117, 3389, 3513, 4284, 4938, 4985, 5037
Bse3DI	GCAATG	4 cut(s)	442, 522, 2074, 3418
BseBI	CCWGG	9 cut(s)	282, 294, 448, 909, 1845, 3084, 3420, 3681, 4044
BseCI	ATCGAT	1 cut(s)	3379
BseMI	GCAATG	4 cut(s)	442, 522, 2074, 3418
BseMII	CTCAG	9 cut(s)	1031, 1133, 1311, 2379, 2875, 3916, 4373, 4416, 4425
BseNI	ACTGG	9 cut(s)	1813, 1910, 3117, 3389, 3513, 4284, 4938, 4985, 5037
BseRI	GAGGAG	9 cut(s)	493, 1159, 1625, 3152, 3341, 4605, 4765, 4925, 5060
BseSI	GKGCMC	2 cut(s)	622, 4567
BseYI	CCCAGC	1 cut(s)	4850
BsgI	GTGCAG	2 cut(s)	1365, 3889
Bsh1285I	CGRYCG	1 cut(s)	205
BshNI	GGYRCC	2 cut(s)	619, 3217
BshVI	ATCGAT	1 cut(s)	3379
BsiEI	CGRYCG	1 cut(s)	205
BsiHKAI	GWGCWC	2 cut(s)	1347, 4567
BsiHKCI	CYCGRG	2 cut(s)	409, 1670
BslFI	GGGAC	7 cut(s)	947, 3331, 3636, 4725, 4792, 5014, 5129
BsmAI	GTCTC	8 cut(s)	140, 1363, 1823, 2250, 3713, 4217, 4233, 4520
BsmBI	CGTCTC	3 cut(s)	140, 1363, 3713
BsmFI	GGGAC	7 cut(s)	947, 3331, 3636, 4725, 4792, 5014, 5129
BsmI	GAATGC	3 cut(s)	611, 3824, 4180
Bso31I	GGTCTC	2 cut(s)	1823, 2250
BsoBI	CYCGRG	2 cut(s)	409, 1670
Bsp119I	TTCGAA	2 cut(s)	1108, 3164
Bsp1286I	GDGCHC	8 cut(s)	404, 416, 622, 862, 1347, 4286, 4440, 4567
Bsp19I	CCATGG	3 cut(s)	3213, 3234, 4036
BspACI	CCGC	6 cut(s)	173, 326, 433, 2065, 2987, 5022
BspCNI	CTCAG	9 cut(s)	1032, 1134, 1312, 2380, 2874, 3915, 4374, 4415, 4426
BspDI	ATCGAT	1 cut(s)	3379
BspHI	TCATGA	4 cut(s)	766, 1099, 1714, 4312
BspMAI	CTGCAG	5 cut(s)	2407, 2452, 3151, 3935, 4206
BspMI	ACCTGC	1 cut(s)	2804
BspPI	GGATC	6 cut(s)	1499, 1607, 2127, 2952, 3410, 4876
BspQI	GCTCTTC	3 cut(s)	1335, 2865, 4188
BspT104I	TTCGAA	2 cut(s)	1108, 3164
BspT107I	GGYRCC	2 cut(s)	619, 3217
BspTI	CTTAAG	1 cut(s)	2969
BspTNI	GGTCTC	2 cut(s)	1823, 2250
BsrDI	GCAATG	4 cut(s)	442, 522, 2074, 3418
BsrFI	RCCGGY	2 cut(s)	298, 373
BsrI	ACTGG	9 cut(s)	1813, 1910, 3117, 3389, 3513, 4284, 4938, 4985, 5037
BssAI	RCCGGY	2 cut(s)	298, 373
BssNAI	GTATAC	1 cut(s)	632
BssNI	GRCGYC	2 cut(s)	104, 3722
BssT1I	CCWWGG	5 cut(s)	473, 3213, 3234, 4036, 4349
Bst1107I	GTATAC	1 cut(s)	632
Bst2UI	CCWGG	9 cut(s)	282, 294, 448, 909, 1845, 3084, 3420, 3681, 4044
Bst6I	CTCTTC	9 cut(s)	1206, 1239, 1335, 2481, 2865, 3015, 3474, 3733, 4188
BstACI	GRCGYC	2 cut(s)	104, 3722
BstAFI	CTTAAG	1 cut(s)	2969
BstAPI	GCANNNNNTGC	4 cut(s)	1964, 2963, 3842, 3939
BstBAI	YACGTR	1 cut(s)	3518
BstBI	TTCGAA	2 cut(s)	1108, 3164
BstC8I	GCNNGC	7 cut(s)	83, 375, 888, 1474, 3371, 4066, 4923
BstDSI	CCRYGG	5 cut(s)	1595, 3213, 3234, 4036, 4365
BstENI	CCTNNNNNAGG	3 cut(s)	128, 3423, 3781
BstH2I	RGCGCY	2 cut(s)	94, 5013
BstHHI	GCGC	2 cut(s)	93, 5012
BstMAI	GTCTC	8 cut(s)	140, 1363, 1823, 2250, 3713, 4217, 4233, 4520
BstMCI	CGRYCG	1 cut(s)	205
BstNI	CCWGG	9 cut(s)	282, 294, 448, 909, 1845, 3084, 3420, 3681, 4044
BstNSI	RCATGY	2 cut(s)	3080, 3992
BstPAI	GACNNNNGTC	1 cut(s)	3006
BstSFI	CTRYAG	5 cut(s)	2403, 2448, 3147, 3931, 4202
BstSLI	GKGCMC	2 cut(s)	622, 4567
BstV2I	GAAGAC	6 cut(s)	107, 857, 2511, 3099, 3927, 4149
BstX2I	RGATCY	2 cut(s)	683, 2132
BstXI	CCANNNNNNTGG	1 cut(s)	4043
BstYI	RGATCY	2 cut(s)	683, 2132
BstZ17I	GTATAC	1 cut(s)	632
Bsu15I	ATCGAT	1 cut(s)	3379
BsuI	GTATCC	2 cut(s)	3505, 4347
BsuTUI	ATCGAT	1 cut(s)	3379
BtgI	CCRYGG	5 cut(s)	1595, 3213, 3234, 4036, 4365
BtgZI	GCGATG	1 cut(s)	4244
BtrI	CACGTC	1 cut(s)	4610
BtsI	GCAGTG	4 cut(s)	897, 2747, 3928, 4965
BveI	ACCTGC	1 cut(s)	2804
Cac8I	GCNNGC	7 cut(s)	83, 375, 888, 1474, 3371, 4066, 4923
CaiI	CAGNNNCTG	5 cut(s)	2081, 3695, 3939, 4268, 4340
CciI	TCATGA	4 cut(s)	766, 1099, 1714, 4312
CfoI	GCGC	2 cut(s)	93, 5012
Cfr10I	RCCGGY	2 cut(s)	298, 373
ClaI	ATCGAT	1 cut(s)	3379
CseI	GACGC	4 cut(s)	112, 1380, 2864, 4167
CspCI	CAANNNNNGTGG	2 cut(s)	4612, 4647
DraI	TTTAAA	1 cut(s)	3702
DrdI	GACNNNNNNGTC	1 cut(s)	4454
DriI	GACNNNNNGTC	2 cut(s)	388, 4246
DseDI	GACNNNNNNGTC	1 cut(s)	4454
EaeI	YGGCCR	4 cut(s)	847, 3097, 3358, 4062
Eam1104I	CTCTTC	9 cut(s)	1206, 1239, 1335, 2481, 2865, 3015, 3474, 3733, 4188
Eam1105I	GACNNNNNGTC	2 cut(s)	388, 4246
EarI	CTCTTC	9 cut(s)	1206, 1239, 1335, 2481, 2865, 3015, 3474, 3733, 4188
EciI	GGCGGA	2 cut(s)	188, 3002
Ecl136II	GAGCTC	1 cut(s)	1345
Eco130I	CCWWGG	5 cut(s)	473, 3213, 3234, 4036, 4349
Eco147I	AGGCCT	2 cut(s)	134, 4082
Eco24I	GRGCYC	6 cut(s)	404, 416, 862, 1347, 4286, 4440
Eco31I	GGTCTC	2 cut(s)	1823, 2250
Eco32I	GATATC	1 cut(s)	4033
Eco47III	AGCGCT	1 cut(s)	5011
Eco53kI	GAGCTC	1 cut(s)	1345
Eco57I	CTGAAG	6 cut(s)	260, 2559, 3090, 5082, 5118, 5169
Eco72I	CACGTG	1 cut(s)	3518
Eco88I	CYCGRG	2 cut(s)	409, 1670
EcoICRI	GAGCTC	1 cut(s)	1345
EcoNI	CCTNNNNNAGG	3 cut(s)	128, 3423, 3781
EcoO109I	RGGNCCY	7 cut(s)	934, 2374, 2533, 3239, 3345, 4688, 5001
EcoRI	GAATTC	2 cut(s)	1110, 1526
EcoRII	CCWGG	9 cut(s)	280, 292, 446, 907, 1843, 3082, 3418, 3679, 4042
EcoRV	GATATC	1 cut(s)	4033
EcoT14I	CCWWGG	5 cut(s)	473, 3213, 3234, 4036, 4349
EcoT22I	ATGCAT	4 cut(s)	613, 2092, 2639, 3277
EcoT38I	GRGCYC	6 cut(s)	404, 416, 862, 1347, 4286, 4440
ErhI	CCWWGG	5 cut(s)	473, 3213, 3234, 4036, 4349
Esp3I	CGTCTC	3 cut(s)	140, 1363, 3713
FalI	AAGNNNNNCTT	6 cut(s)	1368, 1400, 1786, 1818, 2862, 2894
FaqI	GGGAC	7 cut(s)	947, 3331, 3636, 4725, 4792, 5014, 5129
FauNDI	CATATG	2 cut(s)	1879, 4887
FbaI	TGATCA	3 cut(s)	592, 2584, 2779
FblI	GTMKAC	2 cut(s)	631, 5026
FriOI	GRGCYC	6 cut(s)	404, 416, 862, 1347, 4286, 4440
GlaI	GCGC	2 cut(s)	92, 5011
GsaI	CCCAGC	1 cut(s)	4854
GsuI	CTGGAG	7 cut(s)	1145, 1866, 2262, 2544, 3105, 4270, 4990
HaeII	RGCGCY	2 cut(s)	94, 5013
HgaI	GACGC	4 cut(s)	112, 1380, 2864, 4167
HhaI	GCGC	2 cut(s)	93, 5012
Hin1I	GRCGYC	2 cut(s)	104, 3722
Hin6I	GCGC	2 cut(s)	91, 5010
HinP1I	GCGC	2 cut(s)	91, 5010
HincII	GTYRAC	3 cut(s)	2148, 2545, 4212
HindII	GTYRAC	3 cut(s)	2148, 2545, 4212
HindIII	AAGCTT	2 cut(s)	1707, 2318
HpaI	GTTAAC	1 cut(s)	4212
HphI	GGTGA	8 cut(s)	795, 923, 983, 2828, 4480, 4544, 4827, 4907
Hpy99I	CGWCG	2 cut(s)	4737, 4949
Hsp92I	GRCGYC	2 cut(s)	104, 3722
HspAI	GCGC	2 cut(s)	91, 5010
KflI	GGGWCCC	1 cut(s)	934
KpnI	GGTACC	1 cut(s)	3221
KroI	GCCGGC	1 cut(s)	373
KroNI	GCCGGC	1 cut(s)	375
Ksp22I	TGATCA	3 cut(s)	592, 2584, 2779
KspAI	GTTAAC	1 cut(s)	4212
LguI	GCTCTTC	3 cut(s)	1335, 2865, 4188
MfeI	CAATTG	5 cut(s)	345, 542, 596, 777, 3263
MflI	RGATCY	2 cut(s)	683, 2132
MhlI	GDGCHC	8 cut(s)	404, 416, 622, 862, 1347, 4286, 4440, 4567
MlsI	TGGCCA	3 cut(s)	849, 3360, 4064
MluNI	TGGCCA	3 cut(s)	849, 3360, 4064
MlyI	GAGTC	9 cut(s)	170, 500, 2269, 2501, 2596, 2828, 3917, 4394, 4684
MmeI	TCCRAC	4 cut(s)	123, 162, 348, 812
Mox20I	TGGCCA	3 cut(s)	849, 3360, 4064
Mph1103I	ATGCAT	4 cut(s)	613, 2092, 2639, 3277
MroNI	GCCGGC	1 cut(s)	373
MroXI	GAANNNNTTC	4 cut(s)	1944, 3582, 4122, 4197
MscI	TGGCCA	3 cut(s)	849, 3360, 4064
MslI	CAYNNNNRTG	4 cut(s)	765, 1719, 1882, 4493
Msp20I	TGGCCA	3 cut(s)	849, 3360, 4064
MspA1I	CMGCKG	3 cut(s)	2081, 4373, 5022
MspCI	CTTAAG	1 cut(s)	2969
MunI	CAATTG	5 cut(s)	345, 542, 596, 777, 3263
Mva1269I	GAATGC	3 cut(s)	611, 3824, 4180
MvaI	CCWGG	9 cut(s)	282, 294, 448, 909, 1845, 3084, 3420, 3681, 4044
NaeI	GCCGGC	1 cut(s)	375
NciI	CCSGG	5 cut(s)	276, 288, 605, 2128, 2154
NcoI	CCATGG	3 cut(s)	3213, 3234, 4036
NdeI	CATATG	2 cut(s)	1879, 4887
NgoMIV	GCCGGC	1 cut(s)	373
NmuCI	GTSAC	8 cut(s)	227, 380, 492, 870, 2599, 3285, 3504, 4795
NsiI	ATGCAT	4 cut(s)	613, 2092, 2639, 3277
NspI	RCATGY	2 cut(s)	3080, 3992
NspV	TTCGAA	2 cut(s)	1108, 3164
PaeR7I	CTCGAG	2 cut(s)	409, 1670
PagI	TCATGA	4 cut(s)	766, 1099, 1714, 4312
PaqCI	CACCTGC	1 cut(s)	2804
PceI	AGGCCT	2 cut(s)	134, 4082
PciI	ACATGT	2 cut(s)	3076, 3988
PciSI	GCTCTTC	3 cut(s)	1335, 2865, 4188
PcsI	WCGNNNNNNNCGW	2 cut(s)	374, 4732
PctI	GAATGC	3 cut(s)	611, 3824, 4180
PdiI	GCCGGC	1 cut(s)	375
PdmI	GAANNNNTTC	4 cut(s)	1944, 3582, 4122, 4197
PflFI	GACNNNGTC	1 cut(s)	1815
PflMI	CCANNNNNTGG	1 cut(s)	5013
PfoI	TCCNGGA	4 cut(s)	292, 446, 1843, 3082
Ple19I	CGATCG	1 cut(s)	205
PleI	GAGTC	9 cut(s)	169, 499, 2269, 2501, 2596, 2827, 3917, 4394, 4683
PmaCI	CACGTG	1 cut(s)	3518
PmlI	CACGTG	1 cut(s)	3518
PpsI	GAGTC	9 cut(s)	169, 499, 2269, 2501, 2596, 2827, 3917, 4394, 4683
Ppu21I	YACGTR	1 cut(s)	3518
PpuMI	RGGWCCY	7 cut(s)	934, 2374, 2533, 3239, 3345, 4688, 5001
PscI	ACATGT	2 cut(s)	3076, 3988
PshAI	GACNNNNGTC	1 cut(s)	3006
PshBI	ATTAAT	1 cut(s)	1026
PsiI	TTATAA	3 cut(s)	1772, 3123, 5159
Psp124BI	GAGCTC	1 cut(s)	1347
Psp5II	RGGWCCY	7 cut(s)	934, 2374, 2533, 3239, 3345, 4688, 5001
Psp6I	CCWGG	9 cut(s)	280, 292, 446, 907, 1843, 3082, 3418, 3679, 4042
PspCI	CACGTG	1 cut(s)	3518
PspFI	CCCAGC	1 cut(s)	4850
PspGI	CCWGG	9 cut(s)	280, 292, 446, 907, 1843, 3082, 3418, 3679, 4042
PspPPI	RGGWCCY	7 cut(s)	934, 2374, 2533, 3239, 3345, 4688, 5001
PspXI	VCTCGAGB	1 cut(s)	409
PsrI	GAACNNNNNNTAC	2 cut(s)	3978, 4010
PstI	CTGCAG	5 cut(s)	2407, 2452, 3151, 3935, 4206
PstNI	CAGNNNCTG	5 cut(s)	2081, 3695, 3939, 4268, 4340
PsuI	RGATCY	2 cut(s)	683, 2132
PsyI	GACNNNGTC	1 cut(s)	1815
PvuI	CGATCG	1 cut(s)	205
PvuII	CAGCTG	2 cut(s)	2081, 4373
RseI	CAYNNNNRTG	4 cut(s)	765, 1719, 1882, 4493
SacI	GAGCTC	1 cut(s)	1347
SapI	GCTCTTC	3 cut(s)	1335, 2865, 4188
ScaI	AGTACT	3 cut(s)	2767, 3183, 4549
SchI	GAGTC	9 cut(s)	170, 500, 2269, 2501, 2596, 2828, 3917, 4394, 4684
SduI	GDGCHC	8 cut(s)	404, 416, 622, 862, 1347, 4286, 4440, 4567
SfcI	CTRYAG	5 cut(s)	2403, 2448, 3147, 3931, 4202
Sfr274I	CTCGAG	2 cut(s)	409, 1670
SfuI	TTCGAA	2 cut(s)	1108, 3164
SlaI	CTCGAG	2 cut(s)	409, 1670
SmiMI	CAYNNNNRTG	4 cut(s)	765, 1719, 1882, 4493
SseBI	AGGCCT	2 cut(s)	134, 4082
SsiI	CCGC	6 cut(s)	173, 326, 433, 2065, 2987, 5022
SstI	GAGCTC	1 cut(s)	1347
StuI	AGGCCT	2 cut(s)	134, 4082
StyI	CCWWGG	5 cut(s)	473, 3213, 3234, 4036, 4349
TatI	WGTACW	6 cut(s)	2765, 3181, 3686, 3827, 4342, 4547
TseFI	GTSAC	8 cut(s)	227, 380, 492, 870, 2599, 3285, 3504, 4795
Tsp45I	GTSAC	8 cut(s)	227, 380, 492, 870, 2599, 3285, 3504, 4795
TspGWI	ACGGA	1 cut(s)	1584
Tth111I	GACNNNGTC	1 cut(s)	1815
Van91I	CCANNNNNTGG	1 cut(s)	5013
Vha464I	CTTAAG	1 cut(s)	2969
VneI	GTGCAC	1 cut(s)	4563
VspI	ATTAAT	1 cut(s)	1026
XagI	CCTNNNNNAGG	3 cut(s)	128, 3423, 3781
XapI	RAATTY	6 cut(s)	1110, 1526, 1941, 2629, 3035, 3160
XbaI	TCTAGA	1 cut(s)	4321
XceI	RCATGY	2 cut(s)	3080, 3992
XcmI	CCANNNNNNNNNTGG	3 cut(s)	529, 3950, 4372
XhoI	CTCGAG	2 cut(s)	409, 1670
XmiI	GTMKAC	2 cut(s)	631, 5026
XmnI	GAANNNNTTC	4 cut(s)	1944, 3582, 4122, 4197
ZraI	GACGTC	1 cut(s)	3723
ZrmI	AGTACT	3 cut(s)	2767, 3183, 4549
Zsp2I	ATGCAT	4 cut(s)	613, 2092, 2639, 3277

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.