Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0011708

Physical Location & Seq

Genomic Coordinates Reverse (-)

6896583 .. 6905413

Gene Structure 8831 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G006670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5196 bp

ATGGAGGGTTCGGAAGGAGATAATCAGAATCGGAACCATGAGAATAGCAATAGTAAATTGAACAATTCCGGCGAGGGAGGCAAGCCCAAGCGCCAGATGAAGACGCCGTTTCAGCTCGAAACCCTAGAGAAGGCCTATGCGTTGGAGACGTACCCATCGGAGTCGATTAGGGCGGAGCTGTCGGAGAAATTGGGGCTTTCCGATCGGCAGTTGCAGATGTGGTTCTGTCACAGGAGGCTGAAGGATAAGAAGGAAGGAGGTTCGGGTTCGGGTCCGGGTCCAGGACCGGCGAAGAAGCAGAGGAAATCAGTGGCGGTGTTGCCGGAGCCTCCAATTGATGACTTGGCACATGGGTCGGAGCCGGCGAGTGACTATGGGTCGGGTTCGGGCTCCGGCTCGAGCCCATTTGGTCATGCAGACCGCAATGTGGTGTCCAGGAGTGTTGTTGTTGAGGATATGCCAAGGAGGAGGCATTATGAGTCACAGCAGTCGATTACGGAGCTTAGAGCCATTGCTATTGTGGAAGCTCAATTGGGAGAGCCATTAAGGGAAGATGGCCCTGTGCTTGGGATAGAGTTTGATCAATTGCCCCCGGATGCATTCGGGGCACCTTTAGTTGCAGAGCAGCAGAAGCGGCATGATGCAAAACTAAATAAAGGTACTCCGAGATCTCTCCATGAATATCCATATCTTCAAGACCACCCCATTATTCGGCCTGATGTATATGGACAAGTTGCTCAATCTCATTTTCATGATTCAGCAATTGATGGTCCAACTGCAAGAGCTTCACCATTTGCAAGTGGAAATGAACAGTTGTCAAGGGTTCACGGTGGCCACAGTCGGGCTCGTCTTCTGTCACAACAGGAGAAGCAAGCAGTGGCCTTTTCATCTCCTGGTGATGATGGCTTACCTCAAAGGGACCCCTTTATCAATGTTAGAATGAATACTCAATATGGTGAACCCACTATTATTGCACCGGAAAACTCTAATGTATTGTCTGATGGTCAAATTAATGACACTATGCTGAGAATGGAGAGGAAACGCAAGAGTGAAGAAGTCAGAATGGCTAAAGAAGTAGAAGCTCATGAAGTTCGAATTCGGAAGGAGCTGGAGAAACAAGATATTCTGAGGAGAAAGAATGAGGAAAGAATGAGGAAAGAAATGGAAAGGCAAGACCGTGAAAGGCGAAAGGAGGAAGAGAGGTTGATGCGTGAGAGGCAGCGAGAGGAAGAGAGATCAAAGAAGGAGCAAAAACGTGAAATCGAACGAAGGGAGAAGTTTTTGCAAAAGGAACATATAAGAGCTGAGAAAAGGAGGCAAAAGGAAGAGCTCCGAAAAGAGAGAGAGGAAGTGAGACGCAAAGCTGCACTTGAGAAGGCTACTGCAAGAAGGCTTCTTAATAAATCTATGGAACTTTATGAGGATGAGCAACTAGAGCTAATGGAGTTGGCTGCTGCAAGCAAGGGATTATCCTCAATAATTAGTATTGATCCTGACACCAACCTTGATGAATTCAGAGATGATCTGACGGCATTTCCACCGAAGTCTGTGCTATTGAAAAAACCATTTGCAGTTCATCCGTGGATCGATTCAGAGGAGAACATCGGTAACTTTCTCATGGTTTGGAGATTTTTGATTACATTTGCAGATGTTCTTGAGTTATGGCCTTTTACTGTGGATGAGTTTGTTCAAGCTTTTCATGACTATGATTCAAGGTTGTTAGGAGAGATTCATGTTGCTCTCTTGAGGTTGATTATAAAGGATATAGAAGATGTTGCAAGGACACCTTCTACTGGACTAGGTCTCAATCAAAATGGTGCTGCTAATCCTGGAGGTGGACATCCACAGATTGTTGAAGGAGCATATGCGTGGGGATTTGACATAAGGAACTGGCAGAAGCACTTAAATCTGCTAACATGGCCTGAAATTTTTCGCCAACTAGCACTCTCTGCTGGATTTGGGCCACAGTTGAAGAAAAGGAGTATCTCATGGTCATACTTGCCTGATAATGATGAGGGTAAAGGTTGTCGTGATGTAATTTCAACTCTCCGCAATGGTTCAGCAGCTGAAAATGCATTTGCAATAATGCAAGAAAAGGGCTTATTAGCTCCACGGAGATCCAGACATCGGTTGACGCCAGGAACTGTAAAGTTTGCAGCTTTTCATGTCCTTTCTCTTGAGGGAAACAAGGGATTGACGGTGTTAGAACTTGCTGACAAGATTCAGAAATCTGGCCTTCGAGACCTGACAACAAGCAAGACTCCAGAGGCTTCAATTTCAGTTGCTCTGACAAGAGATACTAAGCTTTTTGAAAGAATTGCTCCTTCAACATATCGTGTACGATCTGCTTACAGAAAGGACCCTATTGATGCTGAGGCCATACTTTCTGCAGCTAGGAAGAAGGTTCAGATATTTGAAAATGGGATTTTAGCTGCAGAAGATGTCGATGAGGTTGAAAGAGATGATGCCGAAGAGGTTGAGAGAGATGAAGACTCTGATTGTGATGACGTTGATGAGGACCCTGAAGTTGATGACTTAGCTACTCCAGCCATAGTGAAGAAATCCCCTGATCAGTTTAATGAAGTGACTCCTTTTTCAGAGAATGGACAGGAAAATTTATGCATTGATGTTGCACCAACTGTGCAAAATGAGTTTGATAAGGATGTTTCATCTTTCCCTGTTACTGCTTCCAAAGAGGCAGACGGTCCAAGTGCTTCCTCCAAGCAGTGTGTTTCTGGTGTAGAAATTAGTACTAGCAATCTTGATCAAGAAAATATGGAGATTGATGAGAGCAAGGCAGGTGAGTCATGGGTTCAAGGGCTTACAGAAGGGGATTATTCTGATCTCAGTGTCGAAGAGCGTCTTAGTTCTCTCGTTTCCTTAATTGGTATTGCAAATGAAGGAAACTCGATTCGTGTTGTTCTTGAGGATCGCTTAGAAGCAGCAAATGCTCTTAAGAAGCAAATGTGGGCAGAGGCACAGCTTGACAAAAGTCGCCTAAAAGAAGAGAATGTGAGTAAATTTGATATTCCATCTTTCATGGGAGGCAAAGCTGAAGCACATGTTCCCGGAGTGGAAGACGGCCAAAGTCCTCTACTGGATGTTTATAATAGAATCAATGAGGAAGGAGCTGCAGAAACTCAAAATTCAAATCATTGTTCACAAGTACTTCTGAATGGTGTGCCTGTTGAAAGGGCCATGGTACCTCAAGATACTTCCATGGGTCCTGAAAACATCTTGAATCAGCAATTGGCTTATGCATCAAAAAAGTCACGTTCACAGTTGAAATCATATATTGCCCACAGAGCAGAGGAGATGTATGCTTACAGGTCCCTCCCTCTTGGCCAAGATCGCAGGCATAATCGATACTGGCAATTTGTTGCATCTCCATCGAGCAATGATCCTGGTTCAGGCAGAATCTTTATTGAACTAAACAATGGAAATTGGAGGCTTATTGACACCGAGGAGGCATTTGACACTCTTTTGATGTCACTGGATACACGTGGGATTAGAGAATCCCATTTACGCTTAATGTTGCAAAAGATTGAGGCATCCTTCAAGGAAAATGTTCGTAGAAATGTGCATCCTTCAAGTAGAAATCATGTTAAAAAAGAAGCTGATGAAATGGATTCTAGTCCCGACTACCCTTCAGGTTTCGACAGCCCTGGAAGTACAGTTTCTGCTTTAAATACTGACGTTGGGGAGACGTCCTCTTCTTTCAGAATTGAGCTTAACCGAAATGAAAATGAGAAAAGGGCTGCCTTGAGTAGGTATCAAGATTTTCAGAAGTGGATGTGGAGAGAATGCTTTAGTACATCTGCATTATGTGCCTCGAAATATGGGAAAAAGAGGTGCAGACAGCTCTTTGATTTTTGCGATTTCTGCCTCAGTTGTTACCACTTTGAAGACTCCCACTGCAGTTTCTGCCATCAGACGTTTGGTGCTACCTATGAAAATCTTGACTTTTCTGAACATGTAATACAATGTAAAGAGAGAAGGAACTTGGAAACTTGCGATATCCATATTCCTGGTACTTCTGTTCCTTTGGCCAGCAAATTGCTCAAGGCCTTCGTGGCTCTTGTTGAGGTATCTGTTCCACCAGAAGCCCTTCAATCCTTTTGGACAGAAGACTGTCGAAAGACGTGGGGAGCAAAACTGAATGCGTCATCATCTGTTGAAGATCTTCTGCAGATGTTAACTGTGTTTGAGACTGCGATGAAACGAGACTTTGTGTCATCAAACTTTGCAGCGACTGATGAATTACTGGGCTCCAGCAAACAGTCAGTGATTGCTAATCGTGATTATCTAGACACCAAATCAGTTTCTGTACTTCCTTGGATACCACATACCACGGCAGCTGTGGCTCTGAGGGTTTATGAGATGGACTCAGCAATTACATATATACCGAATGAGAAACCTGAGCCCAATGGTGACAAGGAAGTCGGAGAACATATTAAGATTCCTTTGAGATTCACCCCAATGAGAAATGATAGGGAGATCGAACCAACAGAGACAGACCACAACGAACAAAGTACTCACCTGAAAAGTGCACGAAACAGCCTCAAACGTGGTAGAGGAGGTCGTGAGCAAGGACGTGGTAAAAAGTCCAAATCTGGTGGTAGTCGGCGAAAAGCTAGAGGTAATGAGAACGAGAATATGAGTCAAGGATTAGGACCTGTGGGGCGAAGAACGCAGGGACAAGGGAGTGGACGGGGCCGACGTACTGTTAGAAAGAGGAGAATGAAGAACAGGGCTGTAGAGGGGACACTTATAGGTCATGTGACTGACCTACGCAGTAGCCCTGACAGTGGAGGGGAATCACCCAGAAACTTGGCTGGGGAATGGGATGATGAAAATATCAATATGATCCATATGAAAGGTGATGAACAACGAGAGGAGTATGAGCAAGCAGAGGCACTGGAATCTGATGACGAAGAACAAGCAGTGGGATATGAACAAGGGAACTGGGAGATTGGATTTGATGGGACCTCCAGCGGTTGGAACAGCGGTCTACGAGAACCTAGTGATGAGGAGATGGATGCTTCTGAAGATGATAATAATGGCATTGAGGAAGCGAGAGAAGAAGATTCGGAAGGGGATGTAGATATGAGTGATGCTTCAGATGAAGTCCCAAACAGGATTATAAATGATGATGGCACTGATTCAGCTTCAGACGATGATGATTACAGTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0000976 GO:0000977 GO:0000981 GO:0001012 GO:0001067 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1731

Amino Acids

194.77

Weight (kDa)

5.21

Isoelectric Point (pI)

51.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Homeodomain	PF00046	28 - 84	8e-17	Homeodomain
DDT	PF02791	533 - 588	1.1e-19	DDT domain
HARE-HTH	PF05066	714 - 782	7.4e-19	HB1, ASXL, restriction endonuclease HTH domain
WHIM1	PF15612	932 - 976	3.5e-06	WSTF, HB1, Itc1p, MBD9 motif 1
WSD	PF15613	1109 - 1182	1.2e-21	Williams-Beuren syndrome DDT (WSD), D-TOX E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28420
fragaria_vesca	FvH4_3g08620
malus_domestica	MD05G1310200.v1.1 MD10G1289100.v1.1
prunus_persica	Prupe.4G052600_v2.0.a1
pyrus_communis	pycom05g28660 pycom10g24170
rosa_chinensis	RchiOBHm_Chr5g0009221
rosa_laevigata	RLG00000031635 RLG00000031641
rosa_multiflora	Rmu_sc0006233.1_g000030
rosa_roxburghii	Rroxscaffold_1G00066840 Rroxscaffold_1G00067540
rosa_rugosa	Rorug04G0441600
rosa_samantha	Rh5AG071400 Rh5BG067900 Rh5CG080200 Rh5DG067900
rosa_wichuraiana	Rw5G006670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1757, 3108, 5144
AarI	CACCTGC	1 cut(s)	2789
AasI	GACNNNNNNGTC	1 cut(s)	4439
AatII	GACGTC	1 cut(s)	3710
Acc36I	ACCTGC	1 cut(s)	2789
Acc65I	GGTACC	1 cut(s)	3202
AccB1I	GGYRCC	2 cut(s)	607, 3202
AccB7I	CCANNNNNTGG	1 cut(s)	4998
AccI	GTMKAC	1 cut(s)	5011
AciI	CCGC	7 cut(s)	173, 314, 421, 634, 2050, 4995, 5007
AclWI	GGATC	6 cut(s)	1484, 1592, 2112, 2937, 3395, 4861
AcoI	YGGCCR	4 cut(s)	832, 3082, 3343, 4047
AcsI	RAATTY	6 cut(s)	1095, 1511, 1926, 2614, 3020, 3145
AcuI	CTGAAG	7 cut(s)	260, 2544, 3075, 3633, 5067, 5103, 5154
AcvI	CACGTG	1 cut(s)	3503
AcyI	GRCGYC	3 cut(s)	104, 2135, 3707
AflII	CTTAAG	1 cut(s)	2954
AflIII	ACRYGT	3 cut(s)	3061, 3500, 3973
AhdI	GACNNNNNGTC	2 cut(s)	376, 4231
AjiI	CACGTC	2 cut(s)	4143, 4595
AjnI	CCWGG	8 cut(s)	280, 434, 892, 1828, 2137, 3403, 3664, 4027
AjuI	GAANNNNNNNTTGG	2 cut(s)	3233, 3265
Alw21I	GWGCWC	2 cut(s)	1332, 4552
Alw26I	GTCTC	8 cut(s)	140, 1348, 1808, 2235, 3698, 4202, 4218, 4505
Alw44I	GTGCAC	1 cut(s)	4548
AlwI	GGATC	6 cut(s)	1484, 1592, 2112, 2937, 3395, 4861
AlwNI	CAGNNNCTG	6 cut(s)	2066, 2144, 3680, 3924, 4253, 4325
Ama87I	CYCGRG	1 cut(s)	397
ApaLI	GTGCAC	1 cut(s)	4548
ApoI	RAATTY	6 cut(s)	1095, 1511, 1926, 2614, 3020, 3145
AseI	ATTAAT	1 cut(s)	1011
Asp700I	GAANNNNTTC	4 cut(s)	1929, 3567, 4107, 4182
Asp718I	GGTACC	1 cut(s)	3202
AspLEI	GCGC	1 cut(s)	93
AsuC2I	CCSGG	3 cut(s)	276, 593, 3069
AsuHPI	GGTGA	9 cut(s)	780, 908, 968, 2813, 4442, 4465, 4529, 4812, 4892
AsuII	TTCGAA	1 cut(s)	1093
AvaI	CYCGRG	1 cut(s)	397
BaeGI	GKGCMC	2 cut(s)	610, 4552
BaeI	ACNNNNGTAYC	2 cut(s)	4023, 4056
BalI	TGGCCA	3 cut(s)	834, 3345, 4049
BanI	GGYRCC	2 cut(s)	607, 3202
BanII	GRGCYC	6 cut(s)	392, 404, 847, 1332, 4271, 4425
BbrPI	CACGTG	1 cut(s)	3503
BbsI	GAAGAC	6 cut(s)	107, 842, 2496, 3084, 3912, 4134
Bbv12I	GWGCWC	2 cut(s)	1332, 4552
BbvCI	CCTCAGC	1 cut(s)	2373
BceAI	ACGGC	4 cut(s)	91, 1545, 3097, 4368
BcgI	CGANNNNNNTGC	6 cut(s)	336, 370, 1531, 1565, 3372, 3406
BciT130I	CCWGG	8 cut(s)	282, 436, 894, 1830, 2139, 3405, 3666, 4029
BciVI	GTATCC	2 cut(s)	3490, 4332
BclI	TGATCA	3 cut(s)	580, 2569, 2764
BcnI	CCSGG	3 cut(s)	276, 593, 3069
BcoDI	GTCTC	8 cut(s)	140, 1348, 1808, 2235, 3698, 4202, 4218, 4505
BfmI	CTRYAG	6 cut(s)	2388, 2433, 3132, 3916, 4187, 4755
BfoI	RGCGCY	1 cut(s)	94
BfrI	CTTAAG	1 cut(s)	2954
BfuAI	ACCTGC	1 cut(s)	2789
BfuI	GTATCC	2 cut(s)	3490, 4332
BglI	GCCNNNNNGGC	1 cut(s)	4073
BglII	AGATCT	2 cut(s)	668, 4180
BmcAI	AGTACT	3 cut(s)	2752, 3168, 4534
BmeRI	GACNNNNNGTC	2 cut(s)	376, 4231
BmeT110I	CYCGRG	1 cut(s)	397
BmgBI	CACGTC	2 cut(s)	4143, 4595
BmrI	ACTGGG	2 cut(s)	4274, 4975
BmuI	ACTGGG	2 cut(s)	4274, 4975
BoxI	GACNNNNGTC	1 cut(s)	2991
BpiI	GAAGAC	6 cut(s)	107, 842, 2496, 3084, 3912, 4134
BplI	GAGNNNNNCTC	4 cut(s)	3695, 3727, 4370, 4402
BpmI	CTGGAG	6 cut(s)	1130, 1851, 2247, 2529, 4255, 4975
Bpu10I	CCTNAGC	2 cut(s)	2373, 4419
Bpu14I	TTCGAA	1 cut(s)	1093
BpuEI	CTTGAG	8 cut(s)	1391, 1676, 1765, 2198, 2945, 3192, 3784, 4046
BpuMI	CCSGG	3 cut(s)	276, 593, 3069
Bsa29I	ATCGAT	2 cut(s)	1587, 3364
BsaAI	YACGTR	1 cut(s)	3503
BsaHI	GRCGYC	3 cut(s)	104, 2135, 3707
BsaI	GGTCTC	2 cut(s)	1808, 2235
BsaWI	WCCGGW	1 cut(s)	976
Bse118I	RCCGGY	2 cut(s)	286, 361
Bse1I	ACTGG	8 cut(s)	1798, 1895, 3102, 3374, 3498, 4269, 4923, 4970
Bse3DI	GCAATG	4 cut(s)	430, 510, 2059, 3403
BseBI	CCWGG	8 cut(s)	282, 436, 894, 1830, 2139, 3405, 3666, 4029
BseCI	ATCGAT	2 cut(s)	1587, 3364
BseMI	GCAATG	4 cut(s)	430, 510, 2059, 3403
BseMII	CTCAG	9 cut(s)	1016, 1118, 1296, 2364, 2860, 3901, 4358, 4401, 4410
BseNI	ACTGG	8 cut(s)	1798, 1895, 3102, 3374, 3498, 4269, 4923, 4970
BseRI	GAGGAG	9 cut(s)	481, 1144, 1610, 3326, 3479, 4590, 4750, 4910, 5045
BseSI	GKGCMC	2 cut(s)	610, 4552
BseYI	CCCAGC	1 cut(s)	4835
BsgI	GTGCAG	2 cut(s)	1350, 3874
Bsh1285I	CGRYCG	1 cut(s)	205
BshNI	GGYRCC	2 cut(s)	607, 3202
BshVI	ATCGAT	2 cut(s)	1587, 3364
BsiEI	CGRYCG	1 cut(s)	205
BsiHKAI	GWGCWC	2 cut(s)	1332, 4552
BsiHKCI	CYCGRG	1 cut(s)	397
BsiSI	CCGG	9 cut(s)	69, 275, 287, 323, 362, 393, 593, 977, 3069
BslFI	GGGAC	7 cut(s)	932, 3316, 3621, 4710, 4777, 4999, 5114
BsmAI	GTCTC	8 cut(s)	140, 1348, 1808, 2235, 3698, 4202, 4218, 4505
BsmBI	CGTCTC	3 cut(s)	140, 1348, 3698
BsmFI	GGGAC	7 cut(s)	932, 3316, 3621, 4710, 4777, 4999, 5114
BsmI	GAATGC	3 cut(s)	599, 3809, 4165
Bso31I	GGTCTC	2 cut(s)	1808, 2235
BsoBI	CYCGRG	1 cut(s)	397
Bsp119I	TTCGAA	1 cut(s)	1093
Bsp1286I	GDGCHC	8 cut(s)	392, 404, 610, 847, 1332, 4271, 4425, 4552
Bsp19I	CCATGG	2 cut(s)	3198, 3219
BspACI	CCGC	7 cut(s)	173, 314, 421, 634, 2050, 4995, 5007
BspCNI	CTCAG	9 cut(s)	1017, 1119, 1297, 2365, 2859, 3900, 4359, 4400, 4411
BspDI	ATCGAT	2 cut(s)	1587, 3364
BspHI	TCATGA	3 cut(s)	751, 1084, 1699
BspMAI	CTGCAG	5 cut(s)	2392, 2437, 3136, 3920, 4191
BspMI	ACCTGC	1 cut(s)	2789
BspPI	GGATC	6 cut(s)	1484, 1592, 2112, 2937, 3395, 4861
BspQI	GCTCTTC	2 cut(s)	1320, 2850
BspT104I	TTCGAA	1 cut(s)	1093
BspT107I	GGYRCC	2 cut(s)	607, 3202
BspTI	CTTAAG	1 cut(s)	2954
BspTNI	GGTCTC	2 cut(s)	1808, 2235
BsrDI	GCAATG	4 cut(s)	430, 510, 2059, 3403
BsrFI	RCCGGY	2 cut(s)	286, 361
BsrI	ACTGG	8 cut(s)	1798, 1895, 3102, 3374, 3498, 4269, 4923, 4970
BssAI	RCCGGY	2 cut(s)	286, 361
BssNI	GRCGYC	3 cut(s)	104, 2135, 3707
BssT1I	CCWWGG	4 cut(s)	461, 3198, 3219, 4334
Bst2UI	CCWGG	8 cut(s)	282, 436, 894, 1830, 2139, 3405, 3666, 4029
Bst6I	CTCTTC	7 cut(s)	1191, 1224, 1320, 2466, 2850, 3000, 3718
BstACI	GRCGYC	3 cut(s)	104, 2135, 3707
BstAFI	CTTAAG	1 cut(s)	2954
BstAPI	GCANNNNNTGC	4 cut(s)	1949, 2948, 3827, 3924
BstBAI	YACGTR	1 cut(s)	3503
BstBI	TTCGAA	1 cut(s)	1093
BstC8I	GCNNGC	7 cut(s)	83, 363, 873, 1459, 3356, 4051, 4908
BstDSI	CCRYGG	5 cut(s)	1580, 2111, 3198, 3219, 4350
BstENI	CCTNNNNNAGG	3 cut(s)	128, 3408, 3766
BstH2I	RGCGCY	1 cut(s)	94
BstHHI	GCGC	1 cut(s)	93
BstMAI	GTCTC	8 cut(s)	140, 1348, 1808, 2235, 3698, 4202, 4218, 4505
BstMCI	CGRYCG	1 cut(s)	205
BstNI	CCWGG	8 cut(s)	282, 436, 894, 1830, 2139, 3405, 3666, 4029
BstNSI	RCATGY	2 cut(s)	3065, 3977
BstPAI	GACNNNNGTC	1 cut(s)	2991
BstSFI	CTRYAG	6 cut(s)	2388, 2433, 3132, 3916, 4187, 4755
BstSLI	GKGCMC	2 cut(s)	610, 4552
BstV2I	GAAGAC	6 cut(s)	107, 842, 2496, 3084, 3912, 4134
BstX2I	RGATCY	3 cut(s)	668, 2117, 4180
BstXI	CCANNNNNNTGG	2 cut(s)	4028, 4831
BstYI	RGATCY	3 cut(s)	668, 2117, 4180
Bsu15I	ATCGAT	2 cut(s)	1587, 3364
BsuI	GTATCC	2 cut(s)	3490, 4332
BsuTUI	ATCGAT	2 cut(s)	1587, 3364
BtgI	CCRYGG	5 cut(s)	1580, 2111, 3198, 3219, 4350
BtgZI	GCGATG	1 cut(s)	4229
BtrI	CACGTC	2 cut(s)	4143, 4595
BtsI	GCAGTG	4 cut(s)	882, 2732, 3913, 4950
BveI	ACCTGC	1 cut(s)	2789
Cac8I	GCNNGC	7 cut(s)	83, 363, 873, 1459, 3356, 4051, 4908
CaiI	CAGNNNCTG	6 cut(s)	2066, 2144, 3680, 3924, 4253, 4325
CciI	TCATGA	3 cut(s)	751, 1084, 1699
CfoI	GCGC	1 cut(s)	93
Cfr10I	RCCGGY	2 cut(s)	286, 361
ClaI	ATCGAT	2 cut(s)	1587, 3364
CseI	GACGC	5 cut(s)	112, 1365, 2143, 2849, 4152
CspCI	CAANNNNNGTGG	4 cut(s)	952, 987, 4597, 4632
DraI	TTTAAA	1 cut(s)	3687
DrdI	GACNNNNNNGTC	1 cut(s)	4439
DriI	GACNNNNNGTC	2 cut(s)	376, 4231
DseDI	GACNNNNNNGTC	1 cut(s)	4439
EaeI	YGGCCR	4 cut(s)	832, 3082, 3343, 4047
Eam1104I	CTCTTC	7 cut(s)	1191, 1224, 1320, 2466, 2850, 3000, 3718
Eam1105I	GACNNNNNGTC	2 cut(s)	376, 4231
EarI	CTCTTC	7 cut(s)	1191, 1224, 1320, 2466, 2850, 3000, 3718
EciI	GGCGGA	1 cut(s)	188
Ecl136II	GAGCTC	1 cut(s)	1330
Eco130I	CCWWGG	4 cut(s)	461, 3198, 3219, 4334
Eco147I	AGGCCT	2 cut(s)	134, 4067
Eco24I	GRGCYC	6 cut(s)	392, 404, 847, 1332, 4271, 4425
Eco31I	GGTCTC	2 cut(s)	1808, 2235
Eco32I	GATATC	1 cut(s)	4018
Eco53kI	GAGCTC	1 cut(s)	1330
Eco57I	CTGAAG	7 cut(s)	260, 2544, 3075, 3633, 5067, 5103, 5154
Eco72I	CACGTG	1 cut(s)	3503
Eco88I	CYCGRG	1 cut(s)	397
EcoICRI	GAGCTC	1 cut(s)	1330
EcoNI	CCTNNNNNAGG	3 cut(s)	128, 3408, 3766
EcoO109I	RGGNCCY	7 cut(s)	919, 2359, 2518, 3224, 3330, 4673, 4986
EcoRI	GAATTC	2 cut(s)	1095, 1511
EcoRII	CCWGG	8 cut(s)	280, 434, 892, 1828, 2137, 3403, 3664, 4027
EcoRV	GATATC	1 cut(s)	4018
EcoT14I	CCWWGG	4 cut(s)	461, 3198, 3219, 4334
EcoT22I	ATGCAT	4 cut(s)	601, 2077, 2624, 3262
EcoT38I	GRGCYC	6 cut(s)	392, 404, 847, 1332, 4271, 4425
ErhI	CCWWGG	4 cut(s)	461, 3198, 3219, 4334
Esp3I	CGTCTC	3 cut(s)	140, 1348, 3698
FalI	AAGNNNNNCTT	6 cut(s)	1353, 1385, 1771, 1803, 2847, 2879
FaqI	GGGAC	7 cut(s)	932, 3316, 3621, 4710, 4777, 4999, 5114
FauNDI	CATATG	2 cut(s)	1864, 4872
FbaI	TGATCA	3 cut(s)	580, 2569, 2764
FblI	GTMKAC	1 cut(s)	5011
FriOI	GRGCYC	6 cut(s)	392, 404, 847, 1332, 4271, 4425
GlaI	GCGC	1 cut(s)	92
GsaI	CCCAGC	1 cut(s)	4839
GsuI	CTGGAG	6 cut(s)	1130, 1851, 2247, 2529, 4255, 4975
HaeII	RGCGCY	1 cut(s)	94
HapII	CCGG	9 cut(s)	69, 275, 287, 323, 362, 393, 593, 977, 3069
HgaI	GACGC	5 cut(s)	112, 1365, 2143, 2849, 4152
HhaI	GCGC	1 cut(s)	93
Hin1I	GRCGYC	3 cut(s)	104, 2135, 3707
Hin6I	GCGC	1 cut(s)	91
HinP1I	GCGC	1 cut(s)	91
HincII	GTYRAC	2 cut(s)	2133, 4197
HindII	GTYRAC	2 cut(s)	2133, 4197
HindIII	AAGCTT	2 cut(s)	1692, 2303
HpaI	GTTAAC	1 cut(s)	4197
HpaII	CCGG	9 cut(s)	69, 275, 287, 323, 362, 393, 593, 977, 3069
HphI	GGTGA	9 cut(s)	780, 908, 968, 2813, 4442, 4465, 4529, 4812, 4892
Hpy99I	CGWCG	1 cut(s)	4722
Hsp92I	GRCGYC	3 cut(s)	104, 2135, 3707
HspAI	GCGC	1 cut(s)	91
KflI	GGGWCCC	1 cut(s)	919
KpnI	GGTACC	1 cut(s)	3206
KroI	GCCGGC	1 cut(s)	361
KroNI	GCCGGC	1 cut(s)	363
Ksp22I	TGATCA	3 cut(s)	580, 2569, 2764
KspAI	GTTAAC	1 cut(s)	4197
LguI	GCTCTTC	2 cut(s)	1320, 2850
MfeI	CAATTG	5 cut(s)	333, 530, 584, 762, 3248
MflI	RGATCY	3 cut(s)	668, 2117, 4180
MhlI	GDGCHC	8 cut(s)	392, 404, 610, 847, 1332, 4271, 4425, 4552
MlsI	TGGCCA	3 cut(s)	834, 3345, 4049
MluNI	TGGCCA	3 cut(s)	834, 3345, 4049
MlyI	GAGTC	9 cut(s)	170, 488, 2254, 2486, 2581, 2813, 3902, 4379, 4669
MmeI	TCCRAC	6 cut(s)	123, 162, 336, 797, 4423, 4979
Mox20I	TGGCCA	3 cut(s)	834, 3345, 4049
Mph1103I	ATGCAT	4 cut(s)	601, 2077, 2624, 3262
MroNI	GCCGGC	1 cut(s)	361
MroXI	GAANNNNTTC	4 cut(s)	1929, 3567, 4107, 4182
MscI	TGGCCA	3 cut(s)	834, 3345, 4049
MslI	CAYNNNNRTG	4 cut(s)	750, 1704, 1867, 4478
Msp20I	TGGCCA	3 cut(s)	834, 3345, 4049
MspA1I	CMGCKG	4 cut(s)	2066, 4358, 4995, 5007
MspCI	CTTAAG	1 cut(s)	2954
MspI	CCGG	9 cut(s)	69, 275, 287, 323, 362, 393, 593, 977, 3069
MunI	CAATTG	5 cut(s)	333, 530, 584, 762, 3248
Mva1269I	GAATGC	3 cut(s)	599, 3809, 4165
MvaI	CCWGG	8 cut(s)	282, 436, 894, 1830, 2139, 3405, 3666, 4029
NaeI	GCCGGC	1 cut(s)	363
NciI	CCSGG	3 cut(s)	276, 593, 3069
NcoI	CCATGG	2 cut(s)	3198, 3219
NdeI	CATATG	2 cut(s)	1864, 4872
NgoMIV	GCCGGC	1 cut(s)	361
NmuCI	GTSAC	9 cut(s)	227, 368, 480, 855, 2584, 3270, 3489, 4430, 4780
NsiI	ATGCAT	4 cut(s)	601, 2077, 2624, 3262
NspI	RCATGY	2 cut(s)	3065, 3977
NspV	TTCGAA	1 cut(s)	1093
PaeR7I	CTCGAG	1 cut(s)	397
PagI	TCATGA	3 cut(s)	751, 1084, 1699
PaqCI	CACCTGC	1 cut(s)	2789
PceI	AGGCCT	2 cut(s)	134, 4067
PciI	ACATGT	2 cut(s)	3061, 3973
PciSI	GCTCTTC	2 cut(s)	1320, 2850
PcsI	WCGNNNNNNNCGW	2 cut(s)	362, 4717
PctI	GAATGC	3 cut(s)	599, 3809, 4165
PdiI	GCCGGC	1 cut(s)	363
PdmI	GAANNNNTTC	4 cut(s)	1929, 3567, 4107, 4182
PflFI	GACNNNGTC	1 cut(s)	1800
PflMI	CCANNNNNTGG	1 cut(s)	4998
PfoI	TCCNGGA	4 cut(s)	280, 434, 1828, 3067
Ple19I	CGATCG	1 cut(s)	205
PleI	GAGTC	9 cut(s)	169, 487, 2254, 2486, 2581, 2812, 3902, 4379, 4668
PmaCI	CACGTG	1 cut(s)	3503
PmlI	CACGTG	1 cut(s)	3503
PpsI	GAGTC	9 cut(s)	169, 487, 2254, 2486, 2581, 2812, 3902, 4379, 4668
Ppu21I	YACGTR	1 cut(s)	3503
PpuMI	RGGWCCY	7 cut(s)	919, 2359, 2518, 3224, 3330, 4673, 4986
PscI	ACATGT	2 cut(s)	3061, 3973
PshAI	GACNNNNGTC	1 cut(s)	2991
PshBI	ATTAAT	1 cut(s)	1011
PsiI	TTATAA	3 cut(s)	1757, 3108, 5144
Psp124BI	GAGCTC	1 cut(s)	1332
Psp5II	RGGWCCY	7 cut(s)	919, 2359, 2518, 3224, 3330, 4673, 4986
Psp6I	CCWGG	8 cut(s)	280, 434, 892, 1828, 2137, 3403, 3664, 4027
PspCI	CACGTG	1 cut(s)	3503
PspFI	CCCAGC	1 cut(s)	4835
PspGI	CCWGG	8 cut(s)	280, 434, 892, 1828, 2137, 3403, 3664, 4027
PspPPI	RGGWCCY	7 cut(s)	919, 2359, 2518, 3224, 3330, 4673, 4986
PspXI	VCTCGAGB	1 cut(s)	397
PsrI	GAACNNNNNNTAC	2 cut(s)	3963, 3995
PstI	CTGCAG	5 cut(s)	2392, 2437, 3136, 3920, 4191
PstNI	CAGNNNCTG	6 cut(s)	2066, 2144, 3680, 3924, 4253, 4325
PsuI	RGATCY	3 cut(s)	668, 2117, 4180
PsyI	GACNNNGTC	1 cut(s)	1800
PvuI	CGATCG	1 cut(s)	205
PvuII	CAGCTG	2 cut(s)	2066, 4358
RseI	CAYNNNNRTG	4 cut(s)	750, 1704, 1867, 4478
SacI	GAGCTC	1 cut(s)	1332
SapI	GCTCTTC	2 cut(s)	1320, 2850
ScaI	AGTACT	3 cut(s)	2752, 3168, 4534
SchI	GAGTC	9 cut(s)	170, 488, 2254, 2486, 2581, 2813, 3902, 4379, 4669
SduI	GDGCHC	8 cut(s)	392, 404, 610, 847, 1332, 4271, 4425, 4552
SfcI	CTRYAG	6 cut(s)	2388, 2433, 3132, 3916, 4187, 4755
Sfr274I	CTCGAG	1 cut(s)	397
SfuI	TTCGAA	1 cut(s)	1093
SlaI	CTCGAG	1 cut(s)	397
SmiMI	CAYNNNNRTG	4 cut(s)	750, 1704, 1867, 4478
SseBI	AGGCCT	2 cut(s)	134, 4067
SsiI	CCGC	7 cut(s)	173, 314, 421, 634, 2050, 4995, 5007
SstI	GAGCTC	1 cut(s)	1332
StuI	AGGCCT	2 cut(s)	134, 4067
StyI	CCWWGG	4 cut(s)	461, 3198, 3219, 4334
TatI	WGTACW	6 cut(s)	2750, 3166, 3671, 3812, 4327, 4532
TauI	GCSGC	1 cut(s)	637
TseFI	GTSAC	9 cut(s)	227, 368, 480, 855, 2584, 3270, 3489, 4430, 4780
Tsp45I	GTSAC	9 cut(s)	227, 368, 480, 855, 2584, 3270, 3489, 4430, 4780
TspGWI	ACGGA	3 cut(s)	512, 1569, 2128
Tth111I	GACNNNGTC	1 cut(s)	1800
Van91I	CCANNNNNTGG	1 cut(s)	4998
Vha464I	CTTAAG	1 cut(s)	2954
VneI	GTGCAC	1 cut(s)	4548
VspI	ATTAAT	1 cut(s)	1011
XagI	CCTNNNNNAGG	3 cut(s)	128, 3408, 3766
XapI	RAATTY	6 cut(s)	1095, 1511, 1926, 2614, 3020, 3145
XbaI	TCTAGA	1 cut(s)	4306
XceI	RCATGY	2 cut(s)	3065, 3977
XcmI	CCANNNNNNNNNTGG	3 cut(s)	517, 3935, 4357
XhoI	CTCGAG	1 cut(s)	397
XmiI	GTMKAC	1 cut(s)	5011
XmnI	GAANNNNTTC	4 cut(s)	1929, 3567, 4107, 4182
ZraI	GACGTC	1 cut(s)	3708
ZrmI	AGTACT	3 cut(s)	2752, 3168, 4534
Zsp2I	ATGCAT	4 cut(s)	601, 2077, 2624, 3262

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.