Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0011708

Physical Location & Seq

Genomic Coordinates Reverse (-)

6138794 .. 6147696

Gene Structure 8903 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG080200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5208 bp

ATGGAGGGTTCGGAAGGAGATAATCAGAATCGGAACCATGAGAATAGCAATAGTAAATTGAACAATTCCGGCGAGGGAGGCAAGCCCAAGCGCCAGATGAAGACGCCGTTTCAGCTCGAAACCCTAGAGAAGGCCTATGCGTTGGAGACGTACCCATCGGAGTCGATTAGGGCGGAGCTGTCGGAGAAATTGGGGCTTTCCGATCGGCAGTTGCAGATGTGGTTCTGTCACAGGAGGCTGAAGGATAAGAAGGAAGGAGGTTCGGGTTCGGGTCCGGGTCCGGGTCCGGGTCCAGGACCGGCGAAGAAGCAGAGGAAATCAGTGGCGGTGTTGCCGGAGCCTCCAATTGATGACTTGGCACATGGGTCGGAGCCGGCGAGTGACTATGGGTCGGGTTCGGGCTCCGGCTCGAGCCCATTTGGTCATGCAGACCGCAATGTGGTGTCCAGGAGTGTTGTTGTTGAGGATATGCCAAGGAGGAGGCATTATGAGTCACAGCAGTCGATTACGGAGCTTAGAGCCATTGCTATTGTGGAAGCTCAATTGGGAGAGCCATTAAGGGAAGATGGCCCTGTGCTTGGGATAGAGTTTGATCAATTGCCCCCGGATGCATTCGGGGCACCTCTAGTTGCAGAGCAGCAGAAGCGGCATGATGCAAAACTAAATAAAGGTACTCCGAGATCTCTCCATGAATATCCATATCTTCAAGACCACCCCATTATTCGGCCTGATGTATATGGACAAGTTGCTCAATCTCATTTTCATGATTCAGCAATTGATGGTCCAACTGCAAGAGCTTCACCATTTGCAAGTGGAAATGAACAGTTGTCAAGGGTTCACGGTGGCCACAGTCGGGCTCGTCTTCTGTCACAACAGGAGAAGCAAGCAGTGGCCTTTTCATCTCCTGGTGATGATGGCTTACCTCAAAGGGACCCCTTTATCAATGTTAGAATGAATACTCAATATGGTGAACCCACTATTATTGCACCGGAAAACTCTAATGTATTGTCTGATGGTCAAATTAATGACACTATGCTGAGAATGGAGAGGAAACGCAAGAGTGAAGAAGTCAGAATGGCTAAAGAAGTAGAAGCTCATGAAGTTCGAATTCGGAAGGAGCTGGAGAAACAAGATATTCTGAGGAGAAAGAATGAGGAAAGAATGAGGAAAGAAATGGAAAGGCAAGACCGTGAAAGGCGAAAGGAGGAAGAGAGGTTGATGCGTGAGAGGCAGCGAGAGGAAGAGAGATCAAAGAAGGAGCAAAAACGTGAAATCGAACGAAGGGAGAAGTTTTTGCAAAAAGAACATATAAGAGCTGAGAAAAGGAGGCAAAAGGAAGAGCTCCGAAAAGAGAGAGAGGAAGTGAGACGCAAAGCTGCACTTGAGAAGGCTACTGCAAGAAGGCTTCTTAATAAATCTATGGAACTTTATGAGGATGAGCAACTAGAGCTAATGGAGTTGGCTGCTGCAAGCAAGGGATTATCCTCAATAATTAGTATTGATCCTGACACCAACCTTGATGAATTCAGAGATGATCTGACGGCATTTCCACCGAAGTCTGTGCTATTGAAAAAACCATTTGCAGTTCATCCGTGGATCGATTCAGAGGAGAACATCGGTAACTTTCTCATGGTTTGGAGATTTTTGATTACATTTGCAGATGTTCTTGAGTTATGGCCTTTTACTGTGGATGAGTTTGTTCAAGCTTTTCATGACTATGATTCAAGGTTGTTAGGAGAGATTCATGTTGCTCTCTTGAGGTTGATTATAAAGGATATAGAAGATGTTGCAAGGACACCTTCTACTGGACTAGGTCTCAATCAAAATGGTGCTGCTAATCCTGGAGGTGGACATCCACAGATTGTTGAAGGAGCATATGCGTGGGGATTTGACATAAGGAACTGGCAGAAGCACTTAAATCTGCTAACATGGCCTGAAATTTTTCGCCAACTAGCACTCTCTGCTGGATTTGGGCCACAGTTGAAGAAAAGGAGTATCTCATGGTCGTACTTGCCTGATAATGATGAGGGTAAAGGTTGTCGTGATGTAATTTCAACTCTCCGCAATGGTTCAGCAGCTGAAAATGCATTTGCAATAATGCAAGAAAAGGGCTTATTAGCTCCACGGAGATCCAGACATCGGTTGACGCCAGGAACTGTAAAGTTTGCAGCTTTTCATGTCCTTTCTCTTGAGGGAAACAAGGGATTGACGGTGTTAGAACTTGCTGACAAGATTCAGAAATCTGGCCTTCGAGACCTGACAACAAGCAAGACTCCAGAGGCTTCAATTTCAGTTGCTCTGACAAGAGATACTAAGCTTTTTGAAAGAATTGCTCCTTCAACATATCGTGTACGATCTGCTTACAGAAAGGACCCTATTGATGCTGAGGCCATACTTTCTGCAGCTAGGAAGAAGGTTCAGATATTTGAAAATGGGATTTTAGCTGCAGAAGATGTCGATGAGGTTGAAAGAGATGATGCCGAAGAGGTTGAGAGAGATGAAGACTCTGATTGTGATGACGTTGATGAGGACCCTGAAGTTGATGACTTAGCTACTCCAGCCATAGTGAAGAAATCCCCTGATCAGTTTAATGAAGTGACTCCTTTTTCAGAGAATGGACAGGAAAATTTATGCATTGATGTTGCACCAACTGTGCAAAATGAGTTTGATAAGGATGTTTCATCTTTCCCTGTTACTGCTTCCAAAGAGGCAGACGGTCCAAGTGCTTCCTCCAAGCAGTGTGTTTCTGGTGTAGAAATTAGTACTAGCAATCTTGATCAAGAAAATATGGAGATTGATGAGAGCAAGGCAGGTGAGTCATGGGTTCAAGGGCTCACAGAAGGGGATTATTCTGATCTCAGTGTCGAAGAGCGTCTTAGTTCTCTTGTTTCCTTAATTGGTATTGCAAATGAAGGAAACTCGATTCGTGTTGTTCTTGAGGATCGCTTAGAAGCAGCAAATGCTCTTAAGAAGCAAATGTGGGCAGAGGCACAGCTTGACAAAAGTCGCCTAAAAGAAGAGAATGTGAGTAAATTTGATATTCCATCTTTCATGGGAGGCAAAGCTGAAGCACATGTTCCCGGAGTGGAAGACGGCCAAAGTCCTCTACTGGATGTTTATAATAGAATCAATGAGGAAGGAGCTGCAGAAACTCAAAATTCAAATCATTGTTCACAAGTACTTCTGAATGGTGTGCCTGTTGAAAGGGCCATGGTACCTCAAGATACTTCCATGGGTCCTGAAAACATCTTGAATCAGCAATTGGCTTATGCATCAAAAAAGTCACGTTCACAGTTGAAATCATATATTGCCCACAGAGCAGAGGAGATGTATGCTTACAGGTCCCTCCCTCTTGGCCAAGATCGCAGGCATAATCGATACTGGCAATTTGTTGCATCTGCATCGAGCAATGATCCTGGTTCAGGCAGAATCTTTATTGAACTAAACAATGGAAATTGGAGGCTTATTGACACCGAGGAGGCATTTGACACTCTTTTGATGTCACTGGATACACGTGGGATTAGAGAATCCCATTTACGCTTAATGTTGCAAAAGATTGAGGCATCCTTCAAGGAAAATGTTCGTAGAAATGTGCATCCTTCAAGTAGAAATCATGTTAAAAAAGAAGCTGATGAAATGGATTCTAGTCCCGACTACCCTTCGGGTTTCGACAGCCCTGGAAGTACAGTTTCTGCTTTAAATACTGACGTTGGGGAGACGTCCTCTTCTTTCAGAATTGAGCTTAACCGAAATGAAAATGAGAAAAGGGCTGCCTTGAGTAGGTATCAAGATTTTCAGAAGTGGATGTGGAGAGAATGCTTTAGTACATCTGCATTATGTGCCTCGAAATATGGGAAAAAGAGGTGCAGACAGCTCTTTGATTTTTGCGATTTCTGCCTCAGTTGTTACCACTTTGAAGACTCCCACTGCAGTTTCTGCCATCAGACGTTTGGTGCTACCTATGAAAATCTTGACTTTTCTGAACATGTAATACAATGTAAAGAGAGAAGGAACTTGGAAACTTGCGATATCCATATTCCTGGTACTTCTGTTCCTTTGGCCAGCAAATTGCTCAAGGCCTTCGTGACTCTTGTTGAGGTATCTGTTCCACCAGAAGCCCTTCAATCCTTTTGGACAGAAGACTGTCGAAAGACGTGGGGAGCAAAACTGAATGCGTCATCATCTGTTGAAGAGCTTCTGCAGATGTTAACTGTGTTTGAGACTGCGATGAAACGAGACTTTGTGTCATCAAACTTTGCAGCGACTGATGAATTACTGGGCTCCAGCAAACAGTCAGTGATTGCTAATCGTGATTATCTAGACACCAAATCAGTTTCTGTACTTCCTTGGATACCACATACCACGGCAGCTGTGGCTCTGAGGGTTTATGAGATGGACTCAGCAATTACATATATACCGAATGAGAAACCTGAGCCCAATGGTGACAAGGAAGTCGGAGAACATATTAAGATTCCTTTGAGATTCACCCCAATGAGAAATGATAGGGAGATCGAACCAACAGAGACAGACCACAACGAACAAAGTACTCACCTGAAAAGTGCACGAAACAGCCTCAAACGTGGTAGAGGAGGTCGTGAGCAAGGACGTGGTAAAAAGTCCAAATCTGGTGGTAGTCGGCGAAAAGCTAGAGGTAATGAGAACGAGAATATGAGTCAAGGATTAGGACCTGTGGGGCGAAGAACGCAGGGACAAGGGAGTGGACGGGGCCGACGTACTGTTAGAAAGAGGAGAATGAAGAACAGGGCTGTAGAGGGGACACTTATAGGTCATGTGACTGACCTACGCAGTAGCCCTGACAGTGGAGGGGAATCACCCAGAAACTTGGCTGGGGAATGGGATGATGAAAATATCAATATGATCCATATGAAAGGTGATGAACAACGAGAGGAGTACGAGCAAGCAGAGGCACTGGAATCTGATGACGAAGAACAAGCAGTGGGATATGAACAAGGGAACTGGGAGATTGGATTTGATGGGACCTCCAGCGGTTGGAACAGCGGTCTACGAGAACCTAGTGATGAGGAGATGGATGCTTCTGAAGATGATAATAATGGCATTGAGGAAGCGAGAGAAGAAGATTCGGAAGGGGATGTAGATATGAGTGATGCTTCAGATGAAGTCCCAAACAGGATTATAAATGATGATGGCACTGATTCAGCTTCAGACGATGATGATTACAGTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0000976 GO:0000977 GO:0000981 GO:0001012 GO:0001067 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1735

Amino Acids

195.09

Weight (kDa)

5.21

Isoelectric Point (pI)

51.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Homeodomain	PF00046	28 - 84	8e-17	Homeodomain
DDT	PF02791	537 - 592	1.1e-19	DDT domain
HARE-HTH	PF05066	718 - 786	7.4e-19	HB1, ASXL, restriction endonuclease HTH domain
WHIM1	PF15612	936 - 980	3.5e-06	WSTF, HB1, Itc1p, MBD9 motif 1
WSD	PF15613	1113 - 1186	1.3e-21	Williams-Beuren syndrome DDT (WSD), D-TOX E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0011708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28420
fragaria_vesca	FvH4_3g08620
malus_domestica	MD05G1310200.v1.1 MD10G1289100.v1.1
prunus_persica	Prupe.4G052600_v2.0.a1
pyrus_communis	pycom05g28660 pycom10g24170
rosa_chinensis	RchiOBHm_Chr5g0009221
rosa_laevigata	RLG00000031635 RLG00000031641
rosa_multiflora	Rmu_sc0006233.1_g000030
rosa_roxburghii	Rroxscaffold_1G00066840 Rroxscaffold_1G00067540
rosa_rugosa	Rorug04G0441600
rosa_samantha	Rh5AG071400 Rh5BG067900 Rh5CG080200 Rh5DG067900
rosa_wichuraiana	Rw5G006670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1769, 3120, 5156
AarI	CACCTGC	1 cut(s)	2801
AasI	GACNNNNNNGTC	1 cut(s)	4451
AatII	GACGTC	1 cut(s)	3722
Acc36I	ACCTGC	1 cut(s)	2801
Acc65I	GGTACC	1 cut(s)	3214
AccB1I	GGYRCC	2 cut(s)	619, 3214
AccB7I	CCANNNNNTGG	1 cut(s)	5010
AccI	GTMKAC	1 cut(s)	5023
AciI	CCGC	7 cut(s)	173, 326, 433, 646, 2062, 5007, 5019
AclWI	GGATC	6 cut(s)	1496, 1604, 2124, 2949, 3407, 4873
AcoI	YGGCCR	4 cut(s)	844, 3094, 3355, 4059
AcsI	RAATTY	6 cut(s)	1107, 1523, 1938, 2626, 3032, 3157
AcuI	CTGAAG	6 cut(s)	260, 2556, 3087, 5079, 5115, 5166
AcvI	CACGTG	1 cut(s)	3515
AcyI	GRCGYC	3 cut(s)	104, 2147, 3719
AflII	CTTAAG	1 cut(s)	2966
AflIII	ACRYGT	3 cut(s)	3073, 3512, 3985
AhdI	GACNNNNNGTC	2 cut(s)	388, 4243
AjiI	CACGTC	2 cut(s)	4155, 4607
AjnI	CCWGG	8 cut(s)	292, 446, 904, 1840, 2149, 3415, 3676, 4039
AjuI	GAANNNNNNNTTGG	2 cut(s)	3245, 3277
Alw21I	GWGCWC	2 cut(s)	1344, 4564
Alw26I	GTCTC	8 cut(s)	140, 1360, 1820, 2247, 3710, 4214, 4230, 4517
Alw44I	GTGCAC	1 cut(s)	4560
AlwI	GGATC	6 cut(s)	1496, 1604, 2124, 2949, 3407, 4873
AlwNI	CAGNNNCTG	6 cut(s)	2078, 2156, 3692, 3936, 4265, 4337
Ama87I	CYCGRG	1 cut(s)	409
ApaLI	GTGCAC	1 cut(s)	4560
ApoI	RAATTY	6 cut(s)	1107, 1523, 1938, 2626, 3032, 3157
AseI	ATTAAT	1 cut(s)	1023
Asp700I	GAANNNNTTC	4 cut(s)	1941, 3579, 4119, 4194
Asp718I	GGTACC	1 cut(s)	3214
AspLEI	GCGC	1 cut(s)	93
AsuC2I	CCSGG	5 cut(s)	276, 282, 288, 605, 3081
AsuHPI	GGTGA	9 cut(s)	792, 920, 980, 2825, 4454, 4477, 4541, 4824, 4904
AsuII	TTCGAA	1 cut(s)	1105
AvaI	CYCGRG	1 cut(s)	409
BaeGI	GKGCMC	2 cut(s)	622, 4564
BaeI	ACNNNNGTAYC	2 cut(s)	4035, 4068
BalI	TGGCCA	3 cut(s)	846, 3357, 4061
BanI	GGYRCC	2 cut(s)	619, 3214
BanII	GRGCYC	7 cut(s)	404, 416, 859, 1344, 2835, 4283, 4437
BbrPI	CACGTG	1 cut(s)	3515
BbsI	GAAGAC	6 cut(s)	107, 854, 2508, 3096, 3924, 4146
Bbv12I	GWGCWC	2 cut(s)	1344, 4564
BbvCI	CCTCAGC	1 cut(s)	2385
BceAI	ACGGC	4 cut(s)	91, 1557, 3109, 4380
BcgI	CGANNNNNNTGC	6 cut(s)	348, 382, 1543, 1577, 3384, 3418
BciT130I	CCWGG	8 cut(s)	294, 448, 906, 1842, 2151, 3417, 3678, 4041
BciVI	GTATCC	2 cut(s)	3502, 4344
BclI	TGATCA	3 cut(s)	592, 2581, 2776
BcnI	CCSGG	5 cut(s)	276, 282, 288, 605, 3081
BcoDI	GTCTC	8 cut(s)	140, 1360, 1820, 2247, 3710, 4214, 4230, 4517
BfmI	CTRYAG	6 cut(s)	2400, 2445, 3144, 3928, 4199, 4767
BfoI	RGCGCY	1 cut(s)	94
BfrI	CTTAAG	1 cut(s)	2966
BfuAI	ACCTGC	1 cut(s)	2801
BfuI	GTATCC	2 cut(s)	3502, 4344
BglII	AGATCT	1 cut(s)	680
BmcAI	AGTACT	3 cut(s)	2764, 3180, 4546
BmeRI	GACNNNNNGTC	2 cut(s)	388, 4243
BmeT110I	CYCGRG	1 cut(s)	409
BmgBI	CACGTC	2 cut(s)	4155, 4607
BmrI	ACTGGG	2 cut(s)	4286, 4987
BmuI	ACTGGG	2 cut(s)	4286, 4987
BoxI	GACNNNNGTC	1 cut(s)	3003
BpiI	GAAGAC	6 cut(s)	107, 854, 2508, 3096, 3924, 4146
BplI	GAGNNNNNCTC	4 cut(s)	3707, 3739, 4382, 4414
BpmI	CTGGAG	6 cut(s)	1142, 1863, 2259, 2541, 4267, 4987
Bpu10I	CCTNAGC	2 cut(s)	2385, 4431
Bpu14I	TTCGAA	1 cut(s)	1105
BpuEI	CTTGAG	8 cut(s)	1403, 1688, 1777, 2210, 2957, 3204, 3796, 4058
BpuMI	CCSGG	5 cut(s)	276, 282, 288, 605, 3081
Bsa29I	ATCGAT	2 cut(s)	1599, 3376
BsaAI	YACGTR	1 cut(s)	3515
BsaHI	GRCGYC	3 cut(s)	104, 2147, 3719
BsaI	GGTCTC	2 cut(s)	1820, 2247
BsaWI	WCCGGW	1 cut(s)	988
Bse118I	RCCGGY	2 cut(s)	298, 373
Bse1I	ACTGG	8 cut(s)	1810, 1907, 3114, 3386, 3510, 4281, 4935, 4982
Bse3DI	GCAATG	4 cut(s)	442, 522, 2071, 3415
BseBI	CCWGG	8 cut(s)	294, 448, 906, 1842, 2151, 3417, 3678, 4041
BseCI	ATCGAT	2 cut(s)	1599, 3376
BseMI	GCAATG	4 cut(s)	442, 522, 2071, 3415
BseMII	CTCAG	9 cut(s)	1028, 1130, 1308, 2376, 2872, 3913, 4370, 4413, 4422
BseNI	ACTGG	8 cut(s)	1810, 1907, 3114, 3386, 3510, 4281, 4935, 4982
BseRI	GAGGAG	9 cut(s)	493, 1156, 1622, 3338, 3491, 4602, 4762, 4922, 5057
BseSI	GKGCMC	2 cut(s)	622, 4564
BseYI	CCCAGC	1 cut(s)	4847
BsgI	GTGCAG	2 cut(s)	1362, 3886
Bsh1285I	CGRYCG	1 cut(s)	205
BshNI	GGYRCC	2 cut(s)	619, 3214
BshVI	ATCGAT	2 cut(s)	1599, 3376
BsiEI	CGRYCG	1 cut(s)	205
BsiHKAI	GWGCWC	2 cut(s)	1344, 4564
BsiHKCI	CYCGRG	1 cut(s)	409
BslFI	GGGAC	7 cut(s)	944, 3328, 3633, 4722, 4789, 5011, 5126
BsmAI	GTCTC	8 cut(s)	140, 1360, 1820, 2247, 3710, 4214, 4230, 4517
BsmBI	CGTCTC	3 cut(s)	140, 1360, 3710
BsmFI	GGGAC	7 cut(s)	944, 3328, 3633, 4722, 4789, 5011, 5126
BsmI	GAATGC	3 cut(s)	611, 3821, 4177
Bso31I	GGTCTC	2 cut(s)	1820, 2247
BsoBI	CYCGRG	1 cut(s)	409
Bsp119I	TTCGAA	1 cut(s)	1105
Bsp1286I	GDGCHC	9 cut(s)	404, 416, 622, 859, 1344, 2835, 4283, 4437, 4564
Bsp19I	CCATGG	2 cut(s)	3210, 3231
BspACI	CCGC	7 cut(s)	173, 326, 433, 646, 2062, 5007, 5019
BspCNI	CTCAG	9 cut(s)	1029, 1131, 1309, 2377, 2871, 3912, 4371, 4412, 4423
BspDI	ATCGAT	2 cut(s)	1599, 3376
BspHI	TCATGA	3 cut(s)	763, 1096, 1711
BspMAI	CTGCAG	5 cut(s)	2404, 2449, 3148, 3932, 4203
BspMI	ACCTGC	1 cut(s)	2801
BspPI	GGATC	6 cut(s)	1496, 1604, 2124, 2949, 3407, 4873
BspQI	GCTCTTC	3 cut(s)	1332, 2862, 4185
BspT104I	TTCGAA	1 cut(s)	1105
BspT107I	GGYRCC	2 cut(s)	619, 3214
BspTI	CTTAAG	1 cut(s)	2966
BspTNI	GGTCTC	2 cut(s)	1820, 2247
BsrDI	GCAATG	4 cut(s)	442, 522, 2071, 3415
BsrFI	RCCGGY	2 cut(s)	298, 373
BsrI	ACTGG	8 cut(s)	1810, 1907, 3114, 3386, 3510, 4281, 4935, 4982
BssAI	RCCGGY	2 cut(s)	298, 373
BssNI	GRCGYC	3 cut(s)	104, 2147, 3719
BssT1I	CCWWGG	4 cut(s)	473, 3210, 3231, 4346
Bst2UI	CCWGG	8 cut(s)	294, 448, 906, 1842, 2151, 3417, 3678, 4041
Bst6I	CTCTTC	8 cut(s)	1203, 1236, 1332, 2478, 2862, 3012, 3730, 4185
BstACI	GRCGYC	3 cut(s)	104, 2147, 3719
BstAFI	CTTAAG	1 cut(s)	2966
BstAPI	GCANNNNNTGC	4 cut(s)	1961, 2960, 3839, 3936
BstBAI	YACGTR	1 cut(s)	3515
BstBI	TTCGAA	1 cut(s)	1105
BstC8I	GCNNGC	7 cut(s)	83, 375, 885, 1471, 3368, 4063, 4920
BstDSI	CCRYGG	5 cut(s)	1592, 2123, 3210, 3231, 4362
BstENI	CCTNNNNNAGG	3 cut(s)	128, 3420, 3778
BstH2I	RGCGCY	1 cut(s)	94
BstHHI	GCGC	1 cut(s)	93
BstMAI	GTCTC	8 cut(s)	140, 1360, 1820, 2247, 3710, 4214, 4230, 4517
BstMCI	CGRYCG	1 cut(s)	205
BstNI	CCWGG	8 cut(s)	294, 448, 906, 1842, 2151, 3417, 3678, 4041
BstNSI	RCATGY	2 cut(s)	3077, 3989
BstPAI	GACNNNNGTC	1 cut(s)	3003
BstSFI	CTRYAG	6 cut(s)	2400, 2445, 3144, 3928, 4199, 4767
BstSLI	GKGCMC	2 cut(s)	622, 4564
BstV2I	GAAGAC	6 cut(s)	107, 854, 2508, 3096, 3924, 4146
BstX2I	RGATCY	2 cut(s)	680, 2129
BstXI	CCANNNNNNTGG	2 cut(s)	4040, 4843
BstYI	RGATCY	2 cut(s)	680, 2129
Bsu15I	ATCGAT	2 cut(s)	1599, 3376
BsuI	GTATCC	2 cut(s)	3502, 4344
BsuTUI	ATCGAT	2 cut(s)	1599, 3376
BtgI	CCRYGG	5 cut(s)	1592, 2123, 3210, 3231, 4362
BtgZI	GCGATG	1 cut(s)	4241
BtrI	CACGTC	2 cut(s)	4155, 4607
BtsI	GCAGTG	4 cut(s)	894, 2744, 3925, 4962
BveI	ACCTGC	1 cut(s)	2801
Cac8I	GCNNGC	7 cut(s)	83, 375, 885, 1471, 3368, 4063, 4920
CaiI	CAGNNNCTG	6 cut(s)	2078, 2156, 3692, 3936, 4265, 4337
CciI	TCATGA	3 cut(s)	763, 1096, 1711
CfoI	GCGC	1 cut(s)	93
Cfr10I	RCCGGY	2 cut(s)	298, 373
ClaI	ATCGAT	2 cut(s)	1599, 3376
CseI	GACGC	5 cut(s)	112, 1377, 2155, 2861, 4164
CspCI	CAANNNNNGTGG	4 cut(s)	964, 999, 4609, 4644
DraI	TTTAAA	1 cut(s)	3699
DrdI	GACNNNNNNGTC	1 cut(s)	4451
DriI	GACNNNNNGTC	2 cut(s)	388, 4243
DseDI	GACNNNNNNGTC	1 cut(s)	4451
EaeI	YGGCCR	4 cut(s)	844, 3094, 3355, 4059
Eam1104I	CTCTTC	8 cut(s)	1203, 1236, 1332, 2478, 2862, 3012, 3730, 4185
Eam1105I	GACNNNNNGTC	2 cut(s)	388, 4243
EarI	CTCTTC	8 cut(s)	1203, 1236, 1332, 2478, 2862, 3012, 3730, 4185
EciI	GGCGGA	1 cut(s)	188
Ecl136II	GAGCTC	1 cut(s)	1342
Eco130I	CCWWGG	4 cut(s)	473, 3210, 3231, 4346
Eco147I	AGGCCT	2 cut(s)	134, 4079
Eco24I	GRGCYC	7 cut(s)	404, 416, 859, 1344, 2835, 4283, 4437
Eco31I	GGTCTC	2 cut(s)	1820, 2247
Eco32I	GATATC	1 cut(s)	4030
Eco53kI	GAGCTC	1 cut(s)	1342
Eco57I	CTGAAG	6 cut(s)	260, 2556, 3087, 5079, 5115, 5166
Eco72I	CACGTG	1 cut(s)	3515
Eco88I	CYCGRG	1 cut(s)	409
EcoICRI	GAGCTC	1 cut(s)	1342
EcoNI	CCTNNNNNAGG	3 cut(s)	128, 3420, 3778
EcoO109I	RGGNCCY	7 cut(s)	931, 2371, 2530, 3236, 3342, 4685, 4998
EcoRI	GAATTC	2 cut(s)	1107, 1523
EcoRII	CCWGG	8 cut(s)	292, 446, 904, 1840, 2149, 3415, 3676, 4039
EcoRV	GATATC	1 cut(s)	4030
EcoT14I	CCWWGG	4 cut(s)	473, 3210, 3231, 4346
EcoT22I	ATGCAT	4 cut(s)	613, 2089, 2636, 3274
EcoT38I	GRGCYC	7 cut(s)	404, 416, 859, 1344, 2835, 4283, 4437
ErhI	CCWWGG	4 cut(s)	473, 3210, 3231, 4346
Esp3I	CGTCTC	3 cut(s)	140, 1360, 3710
FalI	AAGNNNNNCTT	6 cut(s)	1365, 1397, 1783, 1815, 2859, 2891
FaqI	GGGAC	7 cut(s)	944, 3328, 3633, 4722, 4789, 5011, 5126
FauNDI	CATATG	2 cut(s)	1876, 4884
FbaI	TGATCA	3 cut(s)	592, 2581, 2776
FblI	GTMKAC	1 cut(s)	5023
FriOI	GRGCYC	7 cut(s)	404, 416, 859, 1344, 2835, 4283, 4437
GlaI	GCGC	1 cut(s)	92
GsaI	CCCAGC	1 cut(s)	4851
GsuI	CTGGAG	6 cut(s)	1142, 1863, 2259, 2541, 4267, 4987
HaeII	RGCGCY	1 cut(s)	94
HgaI	GACGC	5 cut(s)	112, 1377, 2155, 2861, 4164
HhaI	GCGC	1 cut(s)	93
Hin1I	GRCGYC	3 cut(s)	104, 2147, 3719
Hin6I	GCGC	1 cut(s)	91
HinP1I	GCGC	1 cut(s)	91
HincII	GTYRAC	2 cut(s)	2145, 4209
HindII	GTYRAC	2 cut(s)	2145, 4209
HindIII	AAGCTT	2 cut(s)	1704, 2315
HpaI	GTTAAC	1 cut(s)	4209
HphI	GGTGA	9 cut(s)	792, 920, 980, 2825, 4454, 4477, 4541, 4824, 4904
Hpy99I	CGWCG	1 cut(s)	4734
Hsp92I	GRCGYC	3 cut(s)	104, 2147, 3719
HspAI	GCGC	1 cut(s)	91
KflI	GGGWCCC	1 cut(s)	931
KpnI	GGTACC	1 cut(s)	3218
KroI	GCCGGC	1 cut(s)	373
KroNI	GCCGGC	1 cut(s)	375
Ksp22I	TGATCA	3 cut(s)	592, 2581, 2776
KspAI	GTTAAC	1 cut(s)	4209
LguI	GCTCTTC	3 cut(s)	1332, 2862, 4185
MfeI	CAATTG	5 cut(s)	345, 542, 596, 774, 3260
MflI	RGATCY	2 cut(s)	680, 2129
MhlI	GDGCHC	9 cut(s)	404, 416, 622, 859, 1344, 2835, 4283, 4437, 4564
MlsI	TGGCCA	3 cut(s)	846, 3357, 4061
MluNI	TGGCCA	3 cut(s)	846, 3357, 4061
MmeI	TCCRAC	6 cut(s)	123, 162, 348, 809, 4435, 4991
Mox20I	TGGCCA	3 cut(s)	846, 3357, 4061
Mph1103I	ATGCAT	4 cut(s)	613, 2089, 2636, 3274
MroNI	GCCGGC	1 cut(s)	373
MroXI	GAANNNNTTC	4 cut(s)	1941, 3579, 4119, 4194
MscI	TGGCCA	3 cut(s)	846, 3357, 4061
MslI	CAYNNNNRTG	4 cut(s)	762, 1716, 1879, 4490
Msp20I	TGGCCA	3 cut(s)	846, 3357, 4061
MspA1I	CMGCKG	4 cut(s)	2078, 4370, 5007, 5019
MspCI	CTTAAG	1 cut(s)	2966
MunI	CAATTG	5 cut(s)	345, 542, 596, 774, 3260
Mva1269I	GAATGC	3 cut(s)	611, 3821, 4177
MvaI	CCWGG	8 cut(s)	294, 448, 906, 1842, 2151, 3417, 3678, 4041
NaeI	GCCGGC	1 cut(s)	375
NciI	CCSGG	5 cut(s)	276, 282, 288, 605, 3081
NcoI	CCATGG	2 cut(s)	3210, 3231
NdeI	CATATG	2 cut(s)	1876, 4884
NgoMIV	GCCGGC	1 cut(s)	373
NsiI	ATGCAT	4 cut(s)	613, 2089, 2636, 3274
NspI	RCATGY	2 cut(s)	3077, 3989
NspV	TTCGAA	1 cut(s)	1105
PaeR7I	CTCGAG	1 cut(s)	409
PagI	TCATGA	3 cut(s)	763, 1096, 1711
PaqCI	CACCTGC	1 cut(s)	2801
PceI	AGGCCT	2 cut(s)	134, 4079
PciI	ACATGT	2 cut(s)	3073, 3985
PciSI	GCTCTTC	3 cut(s)	1332, 2862, 4185
PcsI	WCGNNNNNNNCGW	2 cut(s)	374, 4729
PctI	GAATGC	3 cut(s)	611, 3821, 4177
PdiI	GCCGGC	1 cut(s)	375
PdmI	GAANNNNTTC	4 cut(s)	1941, 3579, 4119, 4194
PflFI	GACNNNGTC	1 cut(s)	1812
PflMI	CCANNNNNTGG	1 cut(s)	5010
PfoI	TCCNGGA	4 cut(s)	292, 446, 1840, 3079
Ple19I	CGATCG	1 cut(s)	205
PmaCI	CACGTG	1 cut(s)	3515
PmlI	CACGTG	1 cut(s)	3515
Ppu21I	YACGTR	1 cut(s)	3515
PpuMI	RGGWCCY	7 cut(s)	931, 2371, 2530, 3236, 3342, 4685, 4998
PscI	ACATGT	2 cut(s)	3073, 3985
PshAI	GACNNNNGTC	1 cut(s)	3003
PshBI	ATTAAT	1 cut(s)	1023
PsiI	TTATAA	3 cut(s)	1769, 3120, 5156
Psp124BI	GAGCTC	1 cut(s)	1344
Psp5II	RGGWCCY	7 cut(s)	931, 2371, 2530, 3236, 3342, 4685, 4998
Psp6I	CCWGG	8 cut(s)	292, 446, 904, 1840, 2149, 3415, 3676, 4039
PspCI	CACGTG	1 cut(s)	3515
PspFI	CCCAGC	1 cut(s)	4847
PspGI	CCWGG	8 cut(s)	292, 446, 904, 1840, 2149, 3415, 3676, 4039
PspPPI	RGGWCCY	7 cut(s)	931, 2371, 2530, 3236, 3342, 4685, 4998
PspXI	VCTCGAGB	1 cut(s)	409
PsrI	GAACNNNNNNTAC	2 cut(s)	3975, 4007
PstI	CTGCAG	5 cut(s)	2404, 2449, 3148, 3932, 4203
PstNI	CAGNNNCTG	6 cut(s)	2078, 2156, 3692, 3936, 4265, 4337
PsuI	RGATCY	2 cut(s)	680, 2129
PsyI	GACNNNGTC	1 cut(s)	1812
PvuI	CGATCG	1 cut(s)	205
PvuII	CAGCTG	2 cut(s)	2078, 4370
RseI	CAYNNNNRTG	4 cut(s)	762, 1716, 1879, 4490
SacI	GAGCTC	1 cut(s)	1344
SapI	GCTCTTC	3 cut(s)	1332, 2862, 4185
ScaI	AGTACT	3 cut(s)	2764, 3180, 4546
SduI	GDGCHC	9 cut(s)	404, 416, 622, 859, 1344, 2835, 4283, 4437, 4564
SfcI	CTRYAG	6 cut(s)	2400, 2445, 3144, 3928, 4199, 4767
Sfr274I	CTCGAG	1 cut(s)	409
SfuI	TTCGAA	1 cut(s)	1105
SlaI	CTCGAG	1 cut(s)	409
SmiMI	CAYNNNNRTG	4 cut(s)	762, 1716, 1879, 4490
SseBI	AGGCCT	2 cut(s)	134, 4079
SsiI	CCGC	7 cut(s)	173, 326, 433, 646, 2062, 5007, 5019
SstI	GAGCTC	1 cut(s)	1344
StuI	AGGCCT	2 cut(s)	134, 4079
StyI	CCWWGG	4 cut(s)	473, 3210, 3231, 4346
TatI	WGTACW	6 cut(s)	2762, 3178, 3683, 3824, 4339, 4544
TauI	GCSGC	1 cut(s)	649
TspGWI	ACGGA	3 cut(s)	524, 1581, 2140
Tth111I	GACNNNGTC	1 cut(s)	1812
Van91I	CCANNNNNTGG	1 cut(s)	5010
Vha464I	CTTAAG	1 cut(s)	2966
VneI	GTGCAC	1 cut(s)	4560
VspI	ATTAAT	1 cut(s)	1023
XagI	CCTNNNNNAGG	3 cut(s)	128, 3420, 3778
XapI	RAATTY	6 cut(s)	1107, 1523, 1938, 2626, 3032, 3157
XbaI	TCTAGA	1 cut(s)	4318
XceI	RCATGY	2 cut(s)	3077, 3989
XcmI	CCANNNNNNNNNTGG	3 cut(s)	529, 3947, 4369
XhoI	CTCGAG	1 cut(s)	409
XmiI	GTMKAC	1 cut(s)	5023
XmnI	GAANNNNTTC	4 cut(s)	1941, 3579, 4119, 4194
ZraI	GACGTC	1 cut(s)	3720
ZrmI	AGTACT	3 cut(s)	2764, 3180, 4546
Zsp2I	ATGCAT	4 cut(s)	613, 2089, 2636, 3274

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.