Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0004507

Physical Location & Seq

Genomic Coordinates Reverse (-)

40296529 .. 40300070

Gene Structure 3542 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1284600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 846 bp

ATGGCGACCCACTTCTCTCCTGCAACCCCTGTCCGCTTCGTCCTCCAGCCCAAGCAAAACCCTCCCAAAACCCTCCTTTCCATTCGAGATTCTTTTGGTGGAGGGCCGGCGTGTAAGAAATGCCAGCCTAGAACTGTTAATGTGTGCCCATGGAGCTCGTCTGCTAGCTATGTGAGGTCTCAGGATAGTGCTGCGAAGCTTTCGGGCAATGCCATTGTTGTGAAGAAGCAATTGTTCCAAAATAGAGCTGGAATTGTAAGGGCTGCTACTATCGAAGAAATAGAAGCGGAGAAGTCTTTGATAAAGAAAGAAGCTGAAGGAAGGATGGAAAAGACTCTTGAAACTGTACGATCCAATTTCAATTCTATTAGGACAGGGAGGGCAAGCCCGGCCATGCTGGATAAGATTGAGGTTGAATACTATGGAAGCCCAGTTGGCTTGAAGACCATTGCTAATATCAGTTCTCCTGAAGCAAGTTCCTTCTTGGTTCAGCCATACGACAAATCCAGCTTAAAGGCTATAGAGAAGGCCATCGTTAGCTCTGATCTTGGTTTGACTCCAAATAATGATGGAGAAGTAATTCGGTTGACCCTACCTCAACTTACATCAGAGAGAAGGAAGGAATTGTCAAAAATTGTGGCAAAACAGACCGAAGAAGGAAAGGTGGCATTGAGGAACATAAGAAGGGATGCTCTAAAAACTCTTGAAAAACTTGAGAAGGCCAAAAAGCTTTCTGAAGATAATGTCAAGGACTTATCAAGTGATTTGCAGAAATTGACAGATGAGTATGTGAAGAAGCTTGATGCCATATTCAAACAAAAAGAGAAGGAATTGCTGAAGGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002181 GO:0002184 GO:0002376 GO:0003006 GO:0003674 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006415 GO:0006518 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009657 GO:0009658 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009987 GO:0010154 GO:0010467 GO:0016043 GO:0019538 GO:0022411 GO:0022414 GO:0032501 GO:0032502 GO:0032544 GO:0032984 GO:0034641 GO:0034645 GO:0042742 GO:0043021 GO:0043023 GO:0043043 GO:0043167 GO:0043169 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0043624 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045087 GO:0046872 GO:0046914 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0061458 GO:0071704 GO:0071840 GO:0098542 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RRF

Protein Analysis

282

Amino Acids

31.13

Weight (kDa)

9.59

Isoelectric Point (pI)

55.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRF	PF01765	117 - 279	1.4e-65	Ribosome recycling factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0004507)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63190
fragaria_vesca	FvH4_2g23500 FvH4_2g23500
malus_domestica	MD03G1263900.v1.1 MD11G1284600.v1.1
prunus_persica	Prupe.8G240300_v2.0.a1 Prupe.8G240300_v2.0.a1 Prupe.8G240300_v2.0.a1 Prupe.8G240300_v2.0.a1
pyrus_communis	pycom03g21160 pycom11g25210
rosa_chinensis	RchiOBHm_Chr6g0290971
rosa_laevigata	RLG00000007645 RLG00000009086 RLG00000012159 RLG00000013184
rosa_multiflora	Rmu_sc0004191.1_g000054
rosa_roxburghii	Rroxscaffold_7G00176500
rosa_rugosa	Rorug03G0346200 Rorug05G0540500 Rorug06G0216800
rosa_samantha	Rh1CG191500 Rh5AG174800 Rh5DG051200 Rh6AG326500 Rh6BG334100 Rh6CG340200 Rh6DG327400 Rh7BG357000
rosa_wichuraiana	Rw1G021130 Rw2G030470 Rw6G028390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	2 cut(s)	34, 287
AclWI	GGATC	1 cut(s)	345
AcoI	YGGCCR	1 cut(s)	390
AcuI	CTGAAG	3 cut(s)	336, 489, 756
AfaI	GTAC	1 cut(s)	348
AgsI	TTSAA	6 cut(s)	341, 361, 416, 442, 707, 814
AluBI	AGCT	9 cut(s)	156, 168, 199, 248, 314, 510, 540, 730, 799
AluI	AGCT	9 cut(s)	156, 168, 199, 248, 314, 510, 540, 730, 799
Alw21I	GWGCWC	1 cut(s)	158
Alw26I	GTCTC	1 cut(s)	183
AlwI	GGATC	1 cut(s)	345
AoxI	GGCC	4 cut(s)	104, 390, 528, 720
ApeKI	GCWGC	2 cut(s)	191, 263
Asp700I	GAANNNNTTC	1 cut(s)	579
AspS9I	GGNCC	1 cut(s)	104
AsuC2I	CCSGG	1 cut(s)	389
AsuNHI	GCTAGC	1 cut(s)	164
BaeGI	GKGCMC	1 cut(s)	149
BanII	GRGCYC	1 cut(s)	158
BbsI	GAAGAC	1 cut(s)	449
Bbv12I	GWGCWC	1 cut(s)	158
BbvI	GCAGC	2 cut(s)	178, 250
BccI	CCATC	3 cut(s)	319, 539, 563
BcnI	CCSGG	1 cut(s)	389
BcoDI	GTCTC	1 cut(s)	183
BfaI	CTAG	2 cut(s)	129, 165
BfmI	CTRYAG	1 cut(s)	519
BglI	GCCNNNNNGGC	1 cut(s)	435
BisI	GCNGC	2 cut(s)	192, 264
BlsI	GCNGC	2 cut(s)	193, 265
Bme1390I	CCNGG	1 cut(s)	389
BmgT120I	GGNCC	1 cut(s)	104
BmrFI	CCNGG	1 cut(s)	389
BmrI	ACTGGG	1 cut(s)	425
BmsI	GCATC	2 cut(s)	679, 793
BmtI	GCTAGC	1 cut(s)	168
BmuI	ACTGGG	1 cut(s)	425
BpiI	GAAGAC	1 cut(s)	449
BpmI	CTGGAG	1 cut(s)	29
BpuEI	CTTGAG	1 cut(s)	734
BpuMI	CCSGG	1 cut(s)	389
BsaI	GGTCTC	1 cut(s)	183
BsaJI	CCNNGG	1 cut(s)	149
Bse118I	RCCGGY	1 cut(s)	106
Bse1I	ACTGG	1 cut(s)	431
Bse3DI	GCAATG	2 cut(s)	214, 447
BseDI	CCNNGG	1 cut(s)	149
BseGI	GGATG	2 cut(s)	330, 694
BseMI	GCAATG	2 cut(s)	214, 447
BseMII	CTCAG	1 cut(s)	194
BseNI	ACTGG	1 cut(s)	431
BseSI	GKGCMC	1 cut(s)	149
BseXI	GCAGC	2 cut(s)	178, 250
BshFI	GGCC	4 cut(s)	106, 392, 530, 722
BsiHKAI	GWGCWC	1 cut(s)	158
BsiSI	CCGG	2 cut(s)	107, 389
BsmAI	GTCTC	1 cut(s)	183
BsnI	GGCC	4 cut(s)	106, 392, 530, 722
Bso31I	GGTCTC	1 cut(s)	183
Bsp1286I	GDGCHC	2 cut(s)	149, 158
Bsp143I	GATC	2 cut(s)	350, 544
Bsp19I	CCATGG	1 cut(s)	149
BspACI	CCGC	2 cut(s)	34, 287
BspANI	GGCC	4 cut(s)	106, 392, 530, 722
BspCNI	CTCAG	1 cut(s)	193
BspOI	GCTAGC	1 cut(s)	168
BspPI	GGATC	1 cut(s)	345
BspTNI	GGTCTC	1 cut(s)	183
BsrDI	GCAATG	2 cut(s)	214, 447
BsrFI	RCCGGY	1 cut(s)	106
BsrI	ACTGG	1 cut(s)	431
BssAI	RCCGGY	1 cut(s)	106
BssECI	CCNNGG	1 cut(s)	149
BssMI	GATC	2 cut(s)	350, 544
BssT1I	CCWWGG	1 cut(s)	149
Bst4CI	ACNGT	2 cut(s)	136, 346
BstC8I	GCNNGC	4 cut(s)	108, 125, 166, 385
BstDEI	CTNAG	1 cut(s)	180
BstDSI	CCRYGG	1 cut(s)	149
BstF5I	GGATG	2 cut(s)	330, 694
BstKTI	GATC	2 cut(s)	353, 547
BstMAI	GTCTC	1 cut(s)	183
BstMBI	GATC	2 cut(s)	350, 544
BstMWI	GCNNNNNNNGC	3 cut(s)	153, 389, 435
BstSCI	CCNGG	1 cut(s)	387
BstSFI	CTRYAG	1 cut(s)	519
BstSLI	GKGCMC	1 cut(s)	149
BstV1I	GCAGC	2 cut(s)	178, 250
BstV2I	GAAGAC	1 cut(s)	449
BsuRI	GGCC	4 cut(s)	106, 392, 530, 722
BtgI	CCRYGG	1 cut(s)	149
BtsCI	GGATG	2 cut(s)	330, 694
Cac8I	GCNNGC	4 cut(s)	108, 125, 166, 385
Cfr10I	RCCGGY	1 cut(s)	106
Cfr13I	GGNCC	1 cut(s)	104
Csp6I	GTAC	1 cut(s)	347
CspCI	CAANNNNNGTGG	2 cut(s)	618, 653
CviAII	CATG	2 cut(s)	150, 394
CviQI	GTAC	1 cut(s)	347
DdeI	CTNAG	1 cut(s)	180
DpnI	GATC	2 cut(s)	352, 546
DpnII	GATC	2 cut(s)	350, 544
EaeI	YGGCCR	1 cut(s)	390
Ecl136II	GAGCTC	1 cut(s)	156
Eco130I	CCWWGG	1 cut(s)	149
Eco24I	GRGCYC	1 cut(s)	158
Eco31I	GGTCTC	1 cut(s)	183
Eco53kI	GAGCTC	1 cut(s)	156
Eco57I	CTGAAG	3 cut(s)	336, 489, 756
EcoICRI	GAGCTC	1 cut(s)	156
EcoT14I	CCWWGG	1 cut(s)	149
EcoT38I	GRGCYC	1 cut(s)	158
ErhI	CCWWGG	1 cut(s)	149
FaeI	CATG	2 cut(s)	153, 397
FaiI	YATR	9 cut(s)	151, 171, 395, 423, 496, 521, 680, 789, 809
FatI	CATG	2 cut(s)	149, 393
Fnu4HI	GCNGC	2 cut(s)	192, 264
FokI	GGATG	2 cut(s)	337, 701
FriOI	GRGCYC	1 cut(s)	158
Fsp4HI	GCNGC	2 cut(s)	192, 264
FspBI	CTAG	2 cut(s)	129, 165
GluI	GCNGC	2 cut(s)	192, 264
GsuI	CTGGAG	1 cut(s)	29
HaeIII	GGCC	4 cut(s)	106, 392, 530, 722
HapII	CCGG	2 cut(s)	107, 389
Hin1II	CATG	2 cut(s)	153, 397
HincII	GTYRAC	1 cut(s)	588
HindII	GTYRAC	1 cut(s)	588
HindIII	AAGCTT	3 cut(s)	197, 728, 797
HinfI	GANTC	3 cut(s)	89, 334, 556
HpaII	CCGG	2 cut(s)	107, 389
Hpy166II	GTNNAC	1 cut(s)	588
Hpy188I	TCNGA	3 cut(s)	544, 610, 736
Hpy188III	TCNNGA	5 cut(s)	86, 182, 338, 467, 704
Hpy8I	GTNNAC	1 cut(s)	588
HpyCH4III	ACNGT	2 cut(s)	136, 346
HpyCH4V	TGCA	2 cut(s)	23, 769
HpyF10VI	GCNNNNNNNGC	3 cut(s)	153, 389, 435
HpyF3I	CTNAG	1 cut(s)	180
Hsp92II	CATG	2 cut(s)	153, 397
KroI	GCCGGC	1 cut(s)	106
KroNI	GCCGGC	1 cut(s)	108
Kzo9I	GATC	2 cut(s)	350, 544
LmnI	GCTCC	1 cut(s)	153
Lsp1109I	GCAGC	2 cut(s)	178, 250
LweI	GCATC	2 cut(s)	679, 793
MaeI	CTAG	2 cut(s)	129, 165
MalI	GATC	2 cut(s)	352, 546
MboI	GATC	2 cut(s)	350, 544
MboII	GAAGA	6 cut(s)	235, 287, 454, 665, 749, 805
MfeI	CAATTG	1 cut(s)	230
MhlI	GDGCHC	2 cut(s)	149, 158
MluCI	AATT	9 cut(s)	230, 252, 355, 361, 579, 623, 633, 773, 830
MlyI	GAGTC	2 cut(s)	328, 550
MnlI	CCTC	9 cut(s)	53, 72, 83, 95, 168, 372, 403, 606, 666
MroNI	GCCGGC	1 cut(s)	106
MroXI	GAANNNNTTC	1 cut(s)	579
MseI	TTAA	2 cut(s)	138, 512
MslI	CAYNNNNRTG	1 cut(s)	218
MspI	CCGG	2 cut(s)	107, 389
MspR9I	CCNGG	1 cut(s)	389
MunI	CAATTG	1 cut(s)	230
MwoI	GCNNNNNNNGC	3 cut(s)	153, 389, 435
NaeI	GCCGGC	1 cut(s)	108
NciI	CCSGG	1 cut(s)	389
NcoI	CCATGG	1 cut(s)	149
NdeII	GATC	2 cut(s)	350, 544
NgoMIV	GCCGGC	1 cut(s)	106
NheI	GCTAGC	1 cut(s)	164
NlaIII	CATG	2 cut(s)	153, 397
PdiI	GCCGGC	1 cut(s)	108
PdmI	GAANNNNTTC	1 cut(s)	579
PfeI	GAWTC	1 cut(s)	89
PkrI	GCNGC	2 cut(s)	193, 265
PleI	GAGTC	2 cut(s)	328, 550
PpsI	GAGTC	2 cut(s)	328, 550
Psp124BI	GAGCTC	1 cut(s)	158
PspPI	GGNCC	1 cut(s)	104
RsaI	GTAC	1 cut(s)	348
RsaNI	GTAC	1 cut(s)	347
RseI	CAYNNNNRTG	1 cut(s)	218
SacI	GAGCTC	1 cut(s)	158
SaqAI	TTAA	2 cut(s)	138, 512
SatI	GCNGC	2 cut(s)	192, 264
Sau3AI	GATC	2 cut(s)	350, 544
Sau96I	GGNCC	1 cut(s)	104
SchI	GAGTC	2 cut(s)	328, 550
ScrFI	CCNGG	1 cut(s)	389
SduI	GDGCHC	2 cut(s)	149, 158
SfaNI	GCATC	2 cut(s)	679, 793
SfcI	CTRYAG	1 cut(s)	519
SmiMI	CAYNNNNRTG	1 cut(s)	218
SmlI	CTYRAG	1 cut(s)	713
SmoI	CTYRAG	1 cut(s)	713
Sse9I	AATT	9 cut(s)	230, 252, 355, 361, 579, 623, 633, 773, 830
SsiI	CCGC	2 cut(s)	34, 287
SspMI	CTAG	2 cut(s)	129, 165
SstI	GAGCTC	1 cut(s)	158
StyD4I	CCNGG	1 cut(s)	387
StyI	CCWWGG	1 cut(s)	149
TaaI	ACNGT	2 cut(s)	136, 346
TaqI	TCGA	2 cut(s)	85, 273
TaqII	GACCGA	1 cut(s)	665
TasI	AATT	9 cut(s)	230, 252, 355, 361, 579, 623, 633, 773, 830
TfiI	GAWTC	1 cut(s)	89
Tru1I	TTAA	2 cut(s)	138, 512
Tru9I	TTAA	2 cut(s)	138, 512
TseI	GCWGC	2 cut(s)	191, 263
XcmI	CCANNNNNNNNNTGG	1 cut(s)	245
XmnI	GAANNNNTTC	1 cut(s)	579
XspI	CTAG	2 cut(s)	129, 165

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.