Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0004507

Physical Location & Seq

Genomic Coordinates Reverse (-)

72877857 .. 72883658

Gene Structure 5802 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0540500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2394 bp

ATGTCAGGAGAGGGGTACAGCGATGAGACTCATCTGCTGAGATCCTCGAACGACATTAACAAAGACGAGAACGGCGCCGTTTCGCCGGAGGACGACAACGACGGGGACGGCGATGCCGACTTGGAAGCGCAGAGCCGGAAAAGTAGTATCAGTCCGAGCAGTAGAGTCGGTGCTTTTAAGGATCTGCTGCTGAAGCATTTGGACGGCGTCGGAGGGCTTTCCGGTCGCCGGAACAGTTTCAAGAGGCTCGATGGGAATTGGGATAGGGATAGGGATAGGGGTAGGGAGAGTAATCGGAGAGAAAGTAGAGAGCGGCGATCGCCTGTAGATCAGCTGCGTCATAGTAGTAATCATGACTCGCCGGCCGGAATCGATGGGAACGATGAACTGGCCGATAGTGCTCCGCCGGAGTGGGCTTTGCTTCTCCTCGGTTGCATTCTCGGCCTCGCCACCGGTCTCTTTGTTGCCGCGTTTAACAAAGGAGTACATGTTATACACGAATGGGCCTGGGCTGGTACTCCAACTGAAGGTGCTGCATGGCTCCGTCTGCAGAGACTGGGCGATACTTGGCATCGAATACTTTTAGTACCCGTCACAGGTGGGGTCATTGTTGGCATGATGCATGGTTTACTTGAAATATTGGATCAAATAAGACAGTTAAGTTCCTCCCAAGGGAAAGGATTTGATTTACTAGCTGGAGTCTTTCCCACAATAAAGGCCGTCCAGGCAGCTATCACACTAGGTACTGGTTGTTCTTTAGGTCCTGAAGGCCCTAGTGTAGATATTGGAAAATCATGTGCCAATGGGTTCTCATTAATGATGGAAAACAACAGAGAAAGGAAGATTGCCCTCATTGCAGCAGGTGCTGCTGCTGGAATTTCTTCAGGATTTAATGCGGCAGTTGCTGGTTGTTTCTTTGCTATTGAAACGGTGCTAAGGCCACTTCGAGCAGAAAACTCTCCTCCTTTTACTACCGCAATGATTATATTGGCTTCTGTTATATCGTCCACAGTATCTAATGTTTTACTTGGGACACAATCAGCCTTTACAGTGCCTGTTTACGATTTGAAATCAGCTGCTGAATTGCCCCTCTACCTTATATTGGGCATGCTATGTGGTGCTGTGAGTGTAGTCTTCAATCGCTTGGTTGCTTGGTTCACAAAGTTTTTTGATTATATCAAGGAAAGATTTGGCCTTCCTGCGGTTGCATGCCCTGCTTTAGGTGGTTTAGGAGCTGGGTTGATTGCTCTCAAGTATCCGGGAATTCTGTACTGGGGTTTCACAAATGTTGAAGAAATCCTGCATACTGGAAAGACTGCTTCCGCCCCTGGAATCTGGCTGTTAACTCAATTATCAGCAGCCAAAGTTGTAGCAACAGCTTTATGCAAGGGTTCTGGGCTTGTAGGTGGACTTTATGCACCAAGCTTGATGATTGGGGCTGCTGTTGGCGCTGTATTTGGGGGTTCAGCTGCAGAATTGATTAATTCAGCAATTCCAGGAAATGCTGCCGTTGCTCAGCCACAGGCATATGCACTGGTGGGAATGGCTGCTATGCTGGCTTCAGTTTGTTCGGTGCCATTGACGTCAGTTCTCCTTCTGTTTGAGCTGACAAAAGATTACCGAATATTGCTTCCTCTCATGGGGGCTGTTGGATTGGCAATTTGGGTTCCCTCTGTGGTAAACCAGGGAAAGGAGACTGAAGCATCTGATACACGGAATTCTGCGAGAGGTTACTCTTCTGTTTCAGCTGCTGAAGAGAAAGATGAAGTTATTTGGAGACAACATGATAATGGAGATGATATGGAACTGTCTACTATGGGGAATACTTCTGATTCTGAATTAACTGAAGAAATGCTTCTGGAGAATCTAAAGGTTTCGCGGGCCATGTCAAAGAATTATGTGAAGGTTTCTCTGACCGTAACTATTGAAGAGGCAATAAAATCCATGCATGACAATCATCAGAATTGTGTGTTAATTGTTGATGATGAAGATTTTCTGGAGGGAATATTGACATATGGTGATGTTAGACGGTATCAATCAAGGAAGTCTTCTGATACGTTGAAGAGCGATTCTAGTTTTCTGGATGAAAACACCTGTCTTGTTTCCTCTGTATGTACTCGAGAAATCAACTTTCATGGGCGAACCCGAGGTCTATTAACCTGTTATCCGGACACGGGATTATTAATGGCCAAGGAGCTAATGGAGGCAAAAGATATCAAGCAGTTGCCTGTGGTTAAGCGTGGCAGACAACCACCAAAAGAAACGAGGCGAAGACTTGTTGCTATTCTTCATTACGATTCAATCTTGATGTGTCTCAGGGAGGAGATAAATCACAGGAAATCAATTCATCAACATAGAAAAGAGAATCTTGACGAAATAACAAATGGCCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002181 GO:0002184 GO:0002376 GO:0003006 GO:0003674 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006415 GO:0006518 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009657 GO:0009658 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009987 GO:0010154 GO:0010467 GO:0016043 GO:0019538 GO:0022411 GO:0022414 GO:0032501 GO:0032502 GO:0032544 GO:0032984 GO:0034641 GO:0034645 GO:0042742 GO:0043021 GO:0043023 GO:0043043 GO:0043167 GO:0043169 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0043624 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045087 GO:0046872 GO:0046914 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0061458 GO:0071704 GO:0071840 GO:0098542 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RRF

Protein Analysis

797

Amino Acids

85.98

Weight (kDa)

5.77

Isoelectric Point (pI)

42.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	226 - 556	1.1e-70	Voltage gated chloride channel
CBS	PF00571	625 - 679	1.2e-08	CBS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004507)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63190
fragaria_vesca	FvH4_2g23500 FvH4_2g23500
malus_domestica	MD03G1263900.v1.1 MD11G1284600.v1.1
prunus_persica	Prupe.8G240300_v2.0.a1 Prupe.8G240300_v2.0.a1 Prupe.8G240300_v2.0.a1 Prupe.8G240300_v2.0.a1
pyrus_communis	pycom03g21160 pycom11g25210
rosa_chinensis	RchiOBHm_Chr6g0290971
rosa_laevigata	RLG00000007645 RLG00000009086 RLG00000012159 RLG00000013184
rosa_multiflora	Rmu_sc0004191.1_g000054
rosa_roxburghii	Rroxscaffold_7G00176500
rosa_rugosa	Rorug03G0346200 Rorug05G0540500 Rorug06G0216800
rosa_samantha	Rh1CG191500 Rh5AG174800 Rh5DG051200 Rh6AG326500 Rh6BG334100 Rh6CG340200 Rh6DG327400 Rh7BG357000
rosa_wichuraiana	Rw1G021130 Rw2G030470 Rw6G028390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	851
AatII	GACGTC	1 cut(s)	1586
Acc36I	ACCTGC	1 cut(s)	851
AccB1I	GGYRCC	2 cut(s)	74, 1573
AccBSI	CCGCTC	1 cut(s)	313
AccI	GTMKAC	1 cut(s)	1811
AccII	CGCG	2 cut(s)	470, 1879
AccIII	TCCGGA	1 cut(s)	2167
AciI	CCGC	8 cut(s)	313, 404, 468, 896, 975, 1202, 1323, 1879
AclWI	GGATC	3 cut(s)	36, 189, 651
AcoI	YGGCCR	4 cut(s)	363, 390, 2187, 2386
AcsI	RAATTY	3 cut(s)	876, 1263, 1717
AcuI	CTGAAG	8 cut(s)	212, 546, 786, 867, 1545, 1719, 1773, 1866
AcyI	GRCGYC	3 cut(s)	75, 207, 1583
AfaI	GTAC	7 cut(s)	17, 486, 517, 588, 745, 1271, 2116
AflIII	ACRYGT	1 cut(s)	487
AgeI	ACCGGT	1 cut(s)	452
AgsI	TTSAA	9 cut(s)	241, 635, 926, 1069, 1138, 1292, 1928, 2062, 2301
AjnI	CCWGG	5 cut(s)	506, 723, 1327, 1495, 1683
Alw21I	GWGCWC	1 cut(s)	403
Alw26I	GTCTC	6 cut(s)	20, 461, 547, 1688, 1771, 2318
AlwI	GGATC	3 cut(s)	36, 189, 651
AlwNI	CAGNNNCTG	6 cut(s)	556, 866, 905, 1055, 1079, 1751
Ama87I	CYCGRG	2 cut(s)	2118, 2145
Aor13HI	TCCGGA	1 cut(s)	2167
ApoI	RAATTY	3 cut(s)	876, 1263, 1717
ArsI	GACNNNNNNTTYG	2 cut(s)	2134, 2166
AseI	ATTAAT	3 cut(s)	815, 1482, 2183
AsiGI	ACCGGT	1 cut(s)	452
AsiSI	GCGATCGC	1 cut(s)	320
Asp700I	GAANNNNTTC	4 cut(s)	236, 880, 1854, 1992
AspLEI	GCGC	3 cut(s)	77, 130, 1451
AspS9I	GGNCC	4 cut(s)	504, 761, 770, 1881
AsuC2I	CCSGG	1 cut(s)	1260
AsuHPI	GGTGA	1 cut(s)	2030
AvaI	CYCGRG	2 cut(s)	2118, 2145
AvaII	GGWCC	1 cut(s)	761
BalI	TGGCCA	2 cut(s)	2189, 2388
BanI	GGYRCC	2 cut(s)	74, 1573
BbsI	GAAGAC	3 cut(s)	1126, 2040, 2278
Bbv12I	GWGCWC	1 cut(s)	403
BccI	CCATC	3 cut(s)	245, 368, 814
BceAI	ACGGC	6 cut(s)	62, 88, 124, 220, 704, 1493
BcgI	CGANNNNNNTGC	2 cut(s)	148, 182
BciT130I	CCWGG	5 cut(s)	508, 725, 1329, 1497, 1685
BciVI	GTATCC	1 cut(s)	1266
BcnI	CCSGG	1 cut(s)	1260
BcoDI	GTCTC	6 cut(s)	20, 461, 547, 1688, 1771, 2318
BfaI	CTAG	4 cut(s)	692, 740, 774, 2073
BfmI	CTRYAG	3 cut(s)	324, 548, 1470
BfoI	RGCGCY	2 cut(s)	78, 1452
BfuAI	ACCTGC	1 cut(s)	851
BfuI	GTATCC	1 cut(s)	1266
BglI	GCCNNNNNGGC	1 cut(s)	725
BlpI	GCTNAGC	1 cut(s)	1515
Bme1390I	CCNGG	6 cut(s)	508, 725, 1260, 1329, 1497, 1685
Bme18I	GGWCC	1 cut(s)	761
BmeT110I	CYCGRG	2 cut(s)	2118, 2145
BmgT120I	GGNCC	4 cut(s)	504, 761, 770, 1881
BmiI	GGNNCC	4 cut(s)	76, 542, 1575, 1668
BmrFI	CCNGG	6 cut(s)	508, 725, 1260, 1329, 1497, 1685
BmrI	ACTGGG	2 cut(s)	566, 1282
BmsI	GCATC	4 cut(s)	103, 580, 609, 1712
BmuI	ACTGGG	2 cut(s)	566, 1282
BpiI	GAAGAC	3 cut(s)	1126, 2040, 2278
BpmI	CTGGAG	3 cut(s)	717, 1880, 2018
Bpu10I	CCTNAGC	1 cut(s)	935
Bpu1102I	GCTNAGC	1 cut(s)	1515
BpuEI	CTTGAG	1 cut(s)	1235
BpuMI	CCSGG	1 cut(s)	1260
Bsa29I	ATCGAT	1 cut(s)	372
BsaHI	GRCGYC	3 cut(s)	75, 207, 1583
BsaI	GGTCTC	1 cut(s)	461
BsaJI	CCNNGG	7 cut(s)	427, 507, 670, 1327, 1684, 2146, 2190
BsaWI	WCCGGW	3 cut(s)	221, 452, 2167
BsaXI	ACNNNNNCTCC	4 cut(s)	289, 319, 474, 504
Bse118I	RCCGGY	2 cut(s)	361, 452
Bse1I	ACTGG	6 cut(s)	393, 561, 751, 1277, 1312, 1539
Bse3DI	GCAATG	2 cut(s)	852, 984
BseAI	TCCGGA	1 cut(s)	2167
BseBI	CCWGG	5 cut(s)	508, 725, 1329, 1497, 1685
BseCI	ATCGAT	1 cut(s)	372
BseDI	CCNNGG	7 cut(s)	427, 507, 670, 1327, 1684, 2146, 2190
BseGI	GGATG	1 cut(s)	2089
BseMI	GCAATG	2 cut(s)	852, 984
BseMII	CTCAG	3 cut(s)	29, 1529, 2329
BseNI	ACTGG	6 cut(s)	393, 561, 751, 1277, 1312, 1539
BseRI	GAGGAG	3 cut(s)	416, 951, 2336
BseX3I	CGGCCG	1 cut(s)	363
BseYI	CCCAGC	1 cut(s)	1235
Bsh1236I	CGCG	2 cut(s)	470, 1879
Bsh1285I	CGRYCG	3 cut(s)	226, 320, 366
BshNI	GGYRCC	2 cut(s)	74, 1573
BshTI	ACCGGT	1 cut(s)	452
BshVI	ATCGAT	1 cut(s)	372
BsiEI	CGRYCG	3 cut(s)	226, 320, 366
BsiHKAI	GWGCWC	1 cut(s)	403
BsiHKCI	CYCGRG	2 cut(s)	2118, 2145
BslFI	GGGAC	2 cut(s)	119, 1045
BsmAI	GTCTC	6 cut(s)	20, 461, 547, 1688, 1771, 2318
BsmFI	GGGAC	2 cut(s)	119, 1045
BsmI	GAATGC	1 cut(s)	435
Bso31I	GGTCTC	1 cut(s)	461
BsoBI	CYCGRG	2 cut(s)	2118, 2145
Bsp1286I	GDGCHC	1 cut(s)	403
Bsp13I	TCCGGA	1 cut(s)	2167
Bsp143I	GATC	5 cut(s)	41, 181, 317, 328, 643
Bsp1720I	GCTNAGC	1 cut(s)	1515
BspACI	CCGC	8 cut(s)	313, 404, 468, 896, 975, 1202, 1323, 1879
BspCNI	CTCAG	3 cut(s)	30, 1528, 2328
BspDI	ATCGAT	1 cut(s)	372
BspEI	TCCGGA	1 cut(s)	2167
BspFNI	CGCG	2 cut(s)	470, 1879
BspHI	TCATGA	1 cut(s)	352
BspLI	GGNNCC	4 cut(s)	76, 542, 1575, 1668
BspMAI	CTGCAG	2 cut(s)	552, 1474
BspMI	ACCTGC	1 cut(s)	851
BspPI	GGATC	3 cut(s)	36, 189, 651
BspQI	GCTCTTC	1 cut(s)	2057
BspT107I	GGYRCC	2 cut(s)	74, 1573
BspTNI	GGTCTC	1 cut(s)	461
BsrBI	CCGCTC	1 cut(s)	313
BsrDI	GCAATG	2 cut(s)	852, 984
BsrFI	RCCGGY	2 cut(s)	361, 452
BsrI	ACTGG	6 cut(s)	393, 561, 751, 1277, 1312, 1539
BssAI	RCCGGY	2 cut(s)	361, 452
BssECI	CCNNGG	7 cut(s)	427, 507, 670, 1327, 1684, 2146, 2190
BssMI	GATC	5 cut(s)	41, 181, 317, 328, 643
BssNI	GRCGYC	3 cut(s)	75, 207, 1583
BssT1I	CCWWGG	2 cut(s)	670, 2190
Bst2UI	CCWGG	5 cut(s)	508, 725, 1329, 1497, 1685
Bst4CI	ACNGT	8 cut(s)	236, 657, 931, 1012, 1051, 1809, 1918, 2031
Bst6I	CTCTTC	4 cut(s)	1741, 1749, 1923, 2057
BstACI	GRCGYC	3 cut(s)	75, 207, 1583
BstAPI	GCANNNNNTGC	3 cut(s)	863, 866, 1214
BstC8I	GCNNGC	5 cut(s)	363, 1109, 1210, 1557, 1881
BstDEI	CTNAG	4 cut(s)	38, 935, 1515, 2315
BstENI	CCTNNNNNAGG	1 cut(s)	1218
BstF5I	GGATG	1 cut(s)	2089
BstFNI	CGCG	2 cut(s)	470, 1879
BstH2I	RGCGCY	2 cut(s)	78, 1452
BstHHI	GCGC	3 cut(s)	77, 130, 1451
BstKTI	GATC	5 cut(s)	44, 184, 320, 331, 646
BstMAI	GTCTC	6 cut(s)	20, 461, 547, 1688, 1771, 2318
BstMBI	GATC	5 cut(s)	41, 181, 317, 328, 643
BstMCI	CGRYCG	3 cut(s)	226, 320, 366
BstNI	CCWGG	5 cut(s)	508, 725, 1329, 1497, 1685
BstNSI	RCATGY	3 cut(s)	491, 1111, 1212
BstSCI	CCNGG	6 cut(s)	506, 723, 1258, 1327, 1495, 1683
BstSFI	CTRYAG	3 cut(s)	324, 548, 1470
BstUI	CGCG	2 cut(s)	470, 1879
BstV2I	GAAGAC	3 cut(s)	1126, 2040, 2278
BstX2I	RGATCY	2 cut(s)	41, 181
BstYI	RGATCY	2 cut(s)	41, 181
BstZI	CGGCCG	1 cut(s)	363
Bsu15I	ATCGAT	1 cut(s)	372
BsuI	GTATCC	1 cut(s)	1266
BsuTUI	ATCGAT	1 cut(s)	372
BtgZI	GCGATG	2 cut(s)	36, 126
BtsCI	GGATG	1 cut(s)	2089
BtsIMutI	CAGTG	2 cut(s)	1056, 1532
BveI	ACCTGC	1 cut(s)	851
Cac8I	GCNNGC	5 cut(s)	363, 1109, 1210, 1557, 1881
CaiI	CAGNNNCTG	6 cut(s)	556, 866, 905, 1055, 1079, 1751
CciI	TCATGA	1 cut(s)	352
CfoI	GCGC	3 cut(s)	77, 130, 1451
Cfr10I	RCCGGY	2 cut(s)	361, 452
Cfr13I	GGNCC	4 cut(s)	504, 761, 770, 1881
ClaI	ATCGAT	1 cut(s)	372
CseI	GACGC	2 cut(s)	196, 326
Csp6I	GTAC	7 cut(s)	16, 485, 516, 587, 744, 1270, 2115
CspAI	ACCGGT	1 cut(s)	452
CviQI	GTAC	7 cut(s)	16, 485, 516, 587, 744, 1270, 2115
DdeI	CTNAG	4 cut(s)	38, 935, 1515, 2315
DinI	GGCGCC	1 cut(s)	76
DpnI	GATC	5 cut(s)	43, 183, 319, 330, 645
DpnII	GATC	5 cut(s)	41, 181, 317, 328, 643
EaeI	YGGCCR	4 cut(s)	363, 390, 2187, 2386
EagI	CGGCCG	1 cut(s)	363
Eam1104I	CTCTTC	4 cut(s)	1741, 1749, 1923, 2057
EarI	CTCTTC	4 cut(s)	1741, 1749, 1923, 2057
EciI	GGCGGA	2 cut(s)	393, 1312
EclXI	CGGCCG	1 cut(s)	363
Eco130I	CCWWGG	2 cut(s)	670, 2190
Eco31I	GGTCTC	1 cut(s)	461
Eco32I	GATATC	1 cut(s)	2215
Eco47I	GGWCC	1 cut(s)	761
Eco52I	CGGCCG	1 cut(s)	363
Eco57I	CTGAAG	8 cut(s)	212, 546, 786, 867, 1545, 1719, 1773, 1866
Eco88I	CYCGRG	2 cut(s)	2118, 2145
EcoNI	CCTNNNNNAGG	1 cut(s)	1218
EcoO109I	RGGNCCY	2 cut(s)	761, 770
EcoRI	GAATTC	2 cut(s)	1263, 1717
EcoRII	CCWGG	5 cut(s)	506, 723, 1327, 1495, 1683
EcoRV	GATATC	1 cut(s)	2215
EcoT14I	CCWWGG	2 cut(s)	670, 2190
EcoT22I	ATGCAT	2 cut(s)	624, 1950
EgeI	GGCGCC	1 cut(s)	76
EheI	GGCGCC	1 cut(s)	76
ErhI	CCWWGG	2 cut(s)	670, 2190
FalI	AAGNNNNNCTT	6 cut(s)	1839, 1871, 2032, 2064, 2352, 2384
FaqI	GGGAC	2 cut(s)	119, 1045
FauI	CCCGC	1 cut(s)	1872
FauNDI	CATATG	2 cut(s)	1528, 2014
FblI	GTMKAC	1 cut(s)	1811
FokI	GGATG	1 cut(s)	2096
FspBI	CTAG	4 cut(s)	692, 740, 774, 2073
GlaI	GCGC	3 cut(s)	76, 129, 1450
GsaI	CCCAGC	1 cut(s)	1239
GsuI	CTGGAG	3 cut(s)	717, 1880, 2018
HaeII	RGCGCY	2 cut(s)	78, 1452
HgaI	GACGC	2 cut(s)	196, 326
HhaI	GCGC	3 cut(s)	77, 130, 1451
Hin1I	GRCGYC	3 cut(s)	75, 207, 1583
Hin6I	GCGC	3 cut(s)	75, 128, 1449
HinP1I	GCGC	3 cut(s)	75, 128, 1449
HincII	GTYRAC	1 cut(s)	1344
HindII	GTYRAC	1 cut(s)	1344
HindIII	AAGCTT	1 cut(s)	1423
HpaI	GTTAAC	1 cut(s)	1344
HphI	GGTGA	1 cut(s)	2030
Hpy166II	GTNNAC	8 cut(s)	629, 1008, 1060, 1158, 1344, 1409, 1681, 1812
Hpy188I	TCNGA	9 cut(s)	156, 212, 297, 1708, 1831, 1837, 1914, 1962, 2053
Hpy8I	GTNNAC	8 cut(s)	629, 1008, 1060, 1158, 1344, 1409, 1681, 1812
Hpy99I	CGWCG	2 cut(s)	104, 212
HpyAV	CCTTC	5 cut(s)	521, 761, 1205, 1604, 1897
HpyCH4III	ACNGT	8 cut(s)	236, 657, 931, 1012, 1051, 1809, 1918, 2031
HpyCH4IV	ACGT	2 cut(s)	1583, 2057
HpyF3I	CTNAG	4 cut(s)	38, 935, 1515, 2315
HpySE526I	ACGT	2 cut(s)	1583, 2057
Hsp92I	GRCGYC	3 cut(s)	75, 207, 1583
HspAI	GCGC	3 cut(s)	75, 128, 1449
KasI	GGCGCC	1 cut(s)	74
Kpn2I	TCCGGA	1 cut(s)	2167
KroI	GCCGGC	1 cut(s)	361
KroNI	GCCGGC	1 cut(s)	363
KspAI	GTTAAC	1 cut(s)	1344
Kzo9I	GATC	5 cut(s)	41, 181, 317, 328, 643
LguI	GCTCTTC	1 cut(s)	2057
LmnI	GCTCC	4 cut(s)	406, 546, 1232, 2194
LweI	GCATC	4 cut(s)	103, 580, 609, 1712
MaeI	CTAG	4 cut(s)	692, 740, 774, 2073
MaeII	ACGT	2 cut(s)	1583, 2057
MaeIII	GTNAC	3 cut(s)	592, 1730, 1918
MalI	GATC	5 cut(s)	43, 183, 319, 330, 645
MbiI	CCGCTC	1 cut(s)	313
MboI	GATC	5 cut(s)	41, 181, 317, 328, 643
MflI	RGATCY	2 cut(s)	41, 181
MhlI	GDGCHC	1 cut(s)	403
MlsI	TGGCCA	2 cut(s)	2189, 2388
MluNI	TGGCCA	2 cut(s)	2189, 2388
Mly113I	GGCGCC	1 cut(s)	75
MlyI	GAGTC	4 cut(s)	22, 174, 350, 708
MmeI	TCCRAC	3 cut(s)	190, 545, 1630
Mox20I	TGGCCA	2 cut(s)	2189, 2388
Mph1103I	ATGCAT	2 cut(s)	624, 1950
MroI	TCCGGA	1 cut(s)	2167
MroNI	GCCGGC	1 cut(s)	361
MroXI	GAANNNNTTC	4 cut(s)	236, 880, 1854, 1992
MscI	TGGCCA	2 cut(s)	2189, 2388
MslI	CAYNNNNRTG	1 cut(s)	1788
Msp20I	TGGCCA	2 cut(s)	2189, 2388
MspA1I	CMGCKG	4 cut(s)	334, 1076, 1469, 1748
MspR9I	CCNGG	6 cut(s)	508, 725, 1260, 1329, 1497, 1685
Mva1269I	GAATGC	1 cut(s)	435
MvaI	CCWGG	5 cut(s)	508, 725, 1329, 1497, 1685
MvnI	CGCG	2 cut(s)	470, 1879
NaeI	GCCGGC	1 cut(s)	363
NarI	GGCGCC	1 cut(s)	75
NciI	CCSGG	1 cut(s)	1260
NdeI	CATATG	2 cut(s)	1528, 2014
NdeII	GATC	5 cut(s)	41, 181, 317, 328, 643
NgoMIV	GCCGGC	1 cut(s)	361
NlaIV	GGNNCC	4 cut(s)	76, 542, 1575, 1668
NmeAIII	GCCGAG	1 cut(s)	420
NmuCI	GTSAC	1 cut(s)	592
NsiI	ATGCAT	2 cut(s)	624, 1950
NspI	RCATGY	3 cut(s)	491, 1111, 1212
PaeI	GCATGC	2 cut(s)	1111, 1212
PaeR7I	CTCGAG	1 cut(s)	2118
PagI	TCATGA	1 cut(s)	352
PaqCI	CACCTGC	1 cut(s)	851
PciI	ACATGT	1 cut(s)	487
PciSI	GCTCTTC	1 cut(s)	2057
PcsI	WCGNNNNNNNCGW	3 cut(s)	108, 114, 378
PctI	GAATGC	1 cut(s)	435
PdiI	GCCGGC	1 cut(s)	363
PdmI	GAANNNNTTC	4 cut(s)	236, 880, 1854, 1992
PfeI	GAWTC	7 cut(s)	369, 1332, 1832, 1864, 2069, 2297, 2365
PflFI	GACNNNGTC	1 cut(s)	206
PfoI	TCCNGGA	2 cut(s)	1258, 1495
PinAI	ACCGGT	1 cut(s)	452
Ple19I	CGATCG	1 cut(s)	320
PleI	GAGTC	4 cut(s)	22, 173, 350, 707
PluTI	GGCGCC	1 cut(s)	78
PpsI	GAGTC	4 cut(s)	22, 173, 350, 707
PpuMI	RGGWCCY	1 cut(s)	761
PscI	ACATGT	1 cut(s)	487
PshBI	ATTAAT	3 cut(s)	815, 1482, 2183
Psp5II	RGGWCCY	1 cut(s)	761
Psp6I	CCWGG	5 cut(s)	506, 723, 1327, 1495, 1683
PspFI	CCCAGC	1 cut(s)	1235
PspGI	CCWGG	5 cut(s)	506, 723, 1327, 1495, 1683
PspN4I	GGNNCC	4 cut(s)	76, 542, 1575, 1668
PspPI	GGNCC	4 cut(s)	504, 761, 770, 1881
PspPPI	RGGWCCY	1 cut(s)	761
PsrI	GAACNNNNNNTAC	2 cut(s)	736, 768
PstI	CTGCAG	2 cut(s)	552, 1474
PstNI	CAGNNNCTG	6 cut(s)	556, 866, 905, 1055, 1079, 1751
PsuI	RGATCY	2 cut(s)	41, 181
PsyI	GACNNNGTC	1 cut(s)	206
PvuI	CGATCG	1 cut(s)	320
PvuII	CAGCTG	4 cut(s)	334, 1076, 1469, 1748
RgaI	GCGATCGC	1 cut(s)	320
RsaI	GTAC	7 cut(s)	17, 486, 517, 588, 745, 1271, 2116
RsaNI	GTAC	7 cut(s)	16, 485, 516, 587, 744, 1270, 2115
RseI	CAYNNNNRTG	1 cut(s)	1788
SapI	GCTCTTC	1 cut(s)	2057
Sau3AI	GATC	5 cut(s)	41, 181, 317, 328, 643
Sau96I	GGNCC	4 cut(s)	504, 761, 770, 1881
SchI	GAGTC	4 cut(s)	22, 174, 350, 708
ScrFI	CCNGG	6 cut(s)	508, 725, 1260, 1329, 1497, 1685
SduI	GDGCHC	1 cut(s)	403
SfaAI	GCGATCGC	1 cut(s)	320
SfaNI	GCATC	4 cut(s)	103, 580, 609, 1712
SfcI	CTRYAG	3 cut(s)	324, 548, 1470
SfoI	GGCGCC	1 cut(s)	76
Sfr274I	CTCGAG	1 cut(s)	2118
SgfI	GCGATCGC	1 cut(s)	320
SinI	GGWCC	1 cut(s)	761
SlaI	CTCGAG	1 cut(s)	2118
SmiMI	CAYNNNNRTG	1 cut(s)	1788
SmlI	CTYRAG	2 cut(s)	1250, 2118
SmoI	CTYRAG	2 cut(s)	1250, 2118
SphI	GCATGC	2 cut(s)	1111, 1212
SsiI	CCGC	8 cut(s)	313, 404, 468, 896, 975, 1202, 1323, 1879
SspDI	GGCGCC	1 cut(s)	74
SspI	AATATT	3 cut(s)	639, 1626, 2007
SspMI	CTAG	4 cut(s)	692, 740, 774, 2073
StyD4I	CCNGG	6 cut(s)	506, 723, 1258, 1327, 1495, 1683
StyI	CCWWGG	2 cut(s)	670, 2190
TaaI	ACNGT	8 cut(s)	236, 657, 931, 1012, 1051, 1809, 1918, 2031
TaiI	ACGT	2 cut(s)	1586, 2060
TaqI	TCGA	6 cut(s)	47, 249, 372, 574, 946, 2119
TatI	WGTACW	3 cut(s)	484, 1269, 2114
TauI	GCSGC	3 cut(s)	316, 470, 899
TfiI	GAWTC	7 cut(s)	369, 1332, 1832, 1864, 2069, 2297, 2365
TscAI	CASTG	2 cut(s)	1056, 1539
TseFI	GTSAC	1 cut(s)	592
Tsp45I	GTSAC	1 cut(s)	592
TspDTI	ATGAA	7 cut(s)	399, 1779, 2001, 2100, 2123, 2279, 2336
TspGWI	ACGGA	2 cut(s)	533, 1729
TspRI	CASTG	2 cut(s)	1056, 1539
Tth111I	GACNNNGTC	1 cut(s)	206
VpaK11BI	GGWCC	1 cut(s)	761
VspI	ATTAAT	3 cut(s)	815, 1482, 2183
XagI	CCTNNNNNAGG	1 cut(s)	1218
XapI	RAATTY	3 cut(s)	876, 1263, 1717
XceI	RCATGY	3 cut(s)	491, 1111, 1212
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2197
XhoI	CTCGAG	1 cut(s)	2118
XmiI	GTMKAC	1 cut(s)	1811
XmnI	GAANNNNTTC	4 cut(s)	236, 880, 1854, 1992
XspI	CTAG	4 cut(s)	692, 740, 774, 2073
ZraI	GACGTC	1 cut(s)	1584
Zsp2I	ATGCAT	2 cut(s)	624, 1950

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.