MD12G1001200.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011692
Physical Location & Seq
Forward (+)
225321 .. 227615
2295 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1001200.v1.1.491

Sequence Viewer

Length: 1440 bp
ATGGATCCGTCGATGGAAGCACAAACAGAAACAGGCATGGTGGTGAATAGTGGAACATCAAACGAGCTCAAGGGGTTGCCTCCGGGTTTTCGGTTTCATCCCACGGATGAAGAAATTATAACTAGCTATCTCATAAAGAAGGTCATCAACAGCCGCTTCGAAGCTGCTGCAATTGGCGAAGCTGATTTGAACAAGTCCGAACCTTGGGATTTGCCTAAAAAGGCAAAGATGGGAGAGAAGGAGTGGTACTTCTTCTGCCAGAGAGACAGGAAGTATCCTACGGGGATGAGGACGAATCGAGCGACGGAATCAGGGTACTGGAAGGCCACAGGAAAAGATAAAGAGATATTCAATAAGATTAAAAAGCAGCAGCAGCAGGGTGGTAAAGGTGCAGGAGGAGGATGCCTGGTTGGGATGAAGAAGACTCTTGTGTTCTACAGAGGGAGAGCCCCCAAAGGAGAGAAGACCAACTGGGTCATGCATGAATACAGGCTTGATGGCAAAATCCCACCCTACAATGACCTCTCCAAGGCTGCAAAGGACGAATGGGTTGTGTGCAGGGTTTTCCACAAGAACATGGGGCTCAAAAAAACCCTTAGCCCGACTAGGATGAACTCTTTTGGGAATATTGGGGATCATGATCTCCTGGATTGTTCTTCCCTCCCACCTCTTATGGATCCTACTCTTGATACTTCCAACATCATGACCAACAAACATTCTTCAGGCTATGGAGACAATGACTTCAAGCCCGATCCCATTATCACAGTGACACCACTATCGGATCATCATGGAATCAGCACTAACAGTTACCTCAACTACCTTTCATTGGGGGGAGGTGGCAGCGTTCAAGCCCTCAAGCAGAGTTACAACTTTCAGCTGCATCCTACCAGTAACGACAACAGTCACCAAGCCAGTAGTCTCAGTAACCCTTCCAATAAGTTATATCCTCCACCTCGTTATCATTATCATCAGCATCACCAGTTTCAAAATCCTTCTAGTTTCTCTAATTTCCAGCCAAATCAGATGATGAGCAATTCTGATTATTTTCAACAAGGGATGATGAGAGGTGCTCAAGGGCAAGGGTCCGTGTTTCGAACAAGTACTAGTACTAGTAGTGACCATGATGACGATGATCAGGCCATTCCAAGAGCAACAGCTAAAGACAACAACAAAAACAAGATGGAGAATAATATTGAAACATCATCATCTGGCTTGGGAGGAGGAGGCTTAGACAGGCAGTGCAAGGTCGAGCAGTTTTCGACCAACCAGTCTATGTTTAGCCTCTCCCAAGACACCGGATTGAGCACGGATATGAACACTAACGAAATATCATCATCATTTGTTTCAAAGCAAGAATTGGGCAGTACCAACAACTCATCTTATGATCAAGATCCATCTGTTGTTCCCCTTTCCGATATTGAAGACTTGTGGGACTTTTGA

Protein Analysis

480

Amino Acids

53.36

Weight (kDa)

6.4

Isoelectric Point (pI)

34.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 27 - 165 8.1e-48 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011692)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04060 AT5G18270 AT5G18270
fragaria_vesca FvH4_1g27900
malus_domestica MD12G1001200.v1.1 MD14G1001200.v1.1
prunus_persica Prupe.7G001400_v2.0.a1
pyrus_communis pycom12g00110 pycom49g00070
rosa_chinensis RchiOBHm_Chr3g0497471
rosa_laevigata RLG00000022457
rosa_multiflora Rmu_sc0008086.1_g000018
rosa_roxburghii Rroxscaffold_6G00387990
rosa_rugosa Rorug03G0293100
rosa_samantha Rh3AG336800 Rh3BG375100 Rh3CG371100 Rh3DG371800
rosa_wichuraiana Rw3G029600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 119
AasI GACNNNNNNGTC 1 cut(s) 1267
AciI CCGC 1 cut(s) 154
AclWI GGATC 7 cut(s) 12, 642, 671, 684, 746, 789, 1385
AcuI CTGAAG 1 cut(s) 705
AfaI GTAC 5 cut(s) 248, 317, 1102, 1108, 1366
AfiI CCNNNNNNNGG 3 cut(s) 204, 529, 826
AgsI TTSAA 9 cut(s) 190, 352, 745, 848, 986, 1049, 1196, 1347, 1421
AhlI ACTAGT 2 cut(s) 1103, 1109
AjnI CCWGG 2 cut(s) 405, 645
AluBI AGCT 6 cut(s) 67, 126, 164, 182, 877, 1157
AluI AGCT 6 cut(s) 67, 126, 164, 182, 877, 1157
Alw21I GWGCWC 3 cut(s) 69, 1072, 1307
Alw26I GTCTC 3 cut(s) 258, 726, 923
AlwI GGATC 7 cut(s) 12, 642, 671, 684, 746, 789, 1385
AoxI GGCC 2 cut(s) 324, 1137
ApeKI GCWGC 8 cut(s) 164, 167, 367, 370, 373, 533, 840, 877
AspS9I GGNCC 1 cut(s) 1083
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 3 cut(s) 55, 896, 968
AsuII TTCGAA 2 cut(s) 159, 1093
AvaII GGWCC 1 cut(s) 1083
BamHI GGATCC 2 cut(s) 4, 676
BanII GRGCYC 3 cut(s) 69, 451, 585
BarI GAAGNNNNNNTAC 2 cut(s) 230, 262
BbsI GAAGAC 3 cut(s) 428, 470, 1428
Bbv12I GWGCWC 3 cut(s) 69, 1072, 1307
BbvI GCAGC 8 cut(s) 151, 154, 379, 382, 385, 520, 852, 864
BccI CCATC 5 cut(s) 7, 223, 491, 1174, 1402
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BciT130I CCWGG 2 cut(s) 407, 647
BciVI GTATCC 1 cut(s) 285
BclI TGATCA 2 cut(s) 1132, 1384
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 3 cut(s) 258, 726, 923
BcuI ACTAGT 2 cut(s) 1103, 1109
BfaI CTAG 5 cut(s) 123, 606, 996, 1104, 1110
BfmI CTRYAG 1 cut(s) 436
BfuI GTATCC 1 cut(s) 285
BisI GCNGC 9 cut(s) 154, 165, 168, 368, 371, 374, 534, 841, 878
BlsI GCNGC 9 cut(s) 155, 166, 169, 369, 372, 375, 535, 842, 879
BmcAI AGTACT 2 cut(s) 1102, 1108
Bme1390I CCNGG 3 cut(s) 84, 407, 647
Bme18I GGWCC 1 cut(s) 1083
BmgT120I GGNCC 1 cut(s) 1083
BmiI GGNNCC 3 cut(s) 6, 678, 1084
BmrFI CCNGG 3 cut(s) 84, 407, 647
BmrI ACTGGG 1 cut(s) 481
BmsI GCATC 3 cut(s) 392, 889, 982
BmuI ACTGGG 1 cut(s) 481
BoxI GACNNNNGTC 1 cut(s) 900
BpiI GAAGAC 3 cut(s) 428, 470, 1428
BplI GAGNNNNNCTC 2 cut(s) 1054, 1086
Bpu10I CCTNAGC 1 cut(s) 596
Bpu14I TTCGAA 2 cut(s) 159, 1093
BpuEI CTTGAG 3 cut(s) 53, 839, 1056
BpuMI CCSGG 1 cut(s) 84
BsaBI GATNNNNATC 2 cut(s) 639, 1389
BsaJI CCNNGG 3 cut(s) 102, 203, 528
BsaWI WCCGGW 1 cut(s) 1295
Bsc4I CCNNNNNNNGG 3 cut(s) 204, 529, 826
Bse1I ACTGG 6 cut(s) 323, 476, 888, 912, 979, 1267
Bse8I GATNNNNATC 2 cut(s) 639, 1389
BseBI CCWGG 2 cut(s) 407, 647
BseDI CCNNGG 3 cut(s) 102, 203, 528
BseGI GGATG 8 cut(s) 97, 112, 291, 407, 420, 615, 880, 1062
BseJI GATNNNNATC 2 cut(s) 639, 1389
BseLI CCNNNNNNNGG 3 cut(s) 204, 529, 826
BseMII CTCAG 1 cut(s) 934
BseNI ACTGG 6 cut(s) 323, 476, 888, 912, 979, 1267
BseRI GAGGAG 3 cut(s) 411, 1233, 1236
BseXI GCAGC 8 cut(s) 151, 154, 379, 382, 385, 520, 852, 864
BsgI GTGCAG 2 cut(s) 411, 577
BshFI GGCC 2 cut(s) 326, 1139
BsiHKAI GWGCWC 3 cut(s) 69, 1072, 1307
BsiSI CCGG 2 cut(s) 83, 1296
BslI CCNNNNNNNGG 3 cut(s) 204, 529, 826
BsmAI GTCTC 3 cut(s) 258, 726, 923
BsnI GGCC 2 cut(s) 326, 1139
Bsp119I TTCGAA 2 cut(s) 159, 1093
Bsp1286I GDGCHC 5 cut(s) 69, 451, 585, 1072, 1307
Bsp143I GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1384, 1390
BspACI CCGC 1 cut(s) 154
BspANI GGCC 2 cut(s) 326, 1139
BspCNI CTCAG 1 cut(s) 933
BspHI TCATGA 2 cut(s) 637, 702
BspLI GGNNCC 3 cut(s) 6, 678, 1084
BspPI GGATC 7 cut(s) 12, 642, 671, 684, 746, 789, 1385
BspT104I TTCGAA 2 cut(s) 159, 1093
BsrI ACTGG 6 cut(s) 323, 476, 888, 912, 979, 1267
BssECI CCNNGG 3 cut(s) 102, 203, 528
BssMI GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1384, 1390
BssT1I CCWWGG 2 cut(s) 203, 528
Bst2UI CCWGG 2 cut(s) 407, 647
Bst4CI ACNGT 3 cut(s) 766, 806, 902
BstBI TTCGAA 2 cut(s) 159, 1093
BstDEI CTNAG 3 cut(s) 596, 920, 1228
BstDSI CCRYGG 1 cut(s) 102
BstENI CCTNNNNNAGG 1 cut(s) 527
BstF5I GGATG 8 cut(s) 97, 112, 291, 407, 420, 615, 880, 1062
BstKTI GATC 9 cut(s) 7, 637, 643, 679, 754, 784, 1135, 1387, 1393
BstMAI GTCTC 3 cut(s) 258, 726, 923
BstMBI GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1384, 1390
BstMWI GCNNNNNNNGC 1 cut(s) 373
BstNI CCWGG 2 cut(s) 407, 647
BstPAI GACNNNNGTC 1 cut(s) 900
BstSCI CCNGG 3 cut(s) 82, 405, 645
BstSFI CTRYAG 1 cut(s) 436
BstV1I GCAGC 8 cut(s) 151, 154, 379, 382, 385, 520, 852, 864
BstV2I GAAGAC 3 cut(s) 428, 470, 1428
BstX2I RGATCY 3 cut(s) 4, 676, 1390
BstYI RGATCY 3 cut(s) 4, 676, 1390
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 2 cut(s) 326, 1139
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 8 cut(s) 97, 112, 291, 407, 420, 615, 880, 1062
BtsI GCAGTG 1 cut(s) 1244
BtsIMutI CAGTG 2 cut(s) 771, 1244
CciI TCATGA 2 cut(s) 637, 702
Cfr13I GGNCC 1 cut(s) 1083
Csp6I GTAC 5 cut(s) 247, 316, 1101, 1107, 1365
CviAII CATG 8 cut(s) 37, 478, 482, 577, 638, 703, 788, 1121
CviQI GTAC 5 cut(s) 247, 316, 1101, 1107, 1365
DdeI CTNAG 3 cut(s) 596, 920, 1228
DpnI GATC 9 cut(s) 6, 636, 642, 678, 753, 783, 1134, 1386, 1392
DpnII GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1384, 1390
DrdI GACNNNNNNGTC 1 cut(s) 1267
DseDI GACNNNNNNGTC 1 cut(s) 1267
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 2 cut(s) 203, 528
Eco24I GRGCYC 3 cut(s) 69, 451, 585
Eco47I GGWCC 1 cut(s) 1083
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 705
EcoICRI GAGCTC 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 527
EcoRII CCWGG 2 cut(s) 405, 645
EcoT14I CCWWGG 2 cut(s) 203, 528
EcoT22I ATGCAT 1 cut(s) 483
EcoT38I GRGCYC 3 cut(s) 69, 451, 585
ErhI CCWWGG 2 cut(s) 203, 528
FaeI CATG 8 cut(s) 40, 481, 485, 580, 641, 706, 791, 1124
FatI CATG 8 cut(s) 36, 477, 481, 576, 637, 702, 787, 1120
FbaI TGATCA 2 cut(s) 1132, 1384
Fnu4HI GCNGC 9 cut(s) 154, 165, 168, 368, 371, 374, 534, 841, 878
FokI GGATG 8 cut(s) 84, 119, 298, 414, 427, 622, 867, 1069
FriOI GRGCYC 3 cut(s) 69, 451, 585
Fsp4HI GCNGC 9 cut(s) 154, 165, 168, 368, 371, 374, 534, 841, 878
FspBI CTAG 5 cut(s) 123, 606, 996, 1104, 1110
GluI GCNGC 9 cut(s) 154, 165, 168, 368, 371, 374, 534, 841, 878
HaeIII GGCC 2 cut(s) 326, 1139
HapII CCGG 2 cut(s) 83, 1296
Hin1II CATG 8 cut(s) 40, 481, 485, 580, 641, 706, 791, 1124
HinfI GANTC 4 cut(s) 295, 308, 424, 792
HpaII CCGG 2 cut(s) 83, 1296
HphI GGTGA 3 cut(s) 55, 896, 968
Hpy188I TCNGA 5 cut(s) 199, 781, 1023, 1039, 1414
Hpy188III TCNNGA 4 cut(s) 638, 686, 703, 1388
Hpy99I CGWCG 2 cut(s) 13, 307
HpyAV CCTTC 5 cut(s) 133, 232, 316, 939, 1002
HpyCH4III ACNGT 3 cut(s) 766, 806, 902
HpyCH4V TGCA 7 cut(s) 170, 392, 481, 536, 558, 880, 1242
HpyF10VI GCNNNNNNNGC 1 cut(s) 373
HpyF3I CTNAG 3 cut(s) 596, 920, 1228
Hsp92II CATG 8 cut(s) 40, 481, 485, 580, 641, 706, 791, 1124
Ksp22I TGATCA 2 cut(s) 1132, 1384
Kzo9I GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1384, 1390
Lsp1109I GCAGC 8 cut(s) 151, 154, 379, 382, 385, 520, 852, 864
LweI GCATC 3 cut(s) 392, 889, 982
MaeI CTAG 5 cut(s) 123, 606, 996, 1104, 1110
MaeIII GTNAC 7 cut(s) 766, 806, 863, 890, 902, 923, 1115
MalI GATC 9 cut(s) 6, 636, 642, 678, 753, 783, 1134, 1386, 1392
MboI GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1384, 1390
MboII GAAGA 8 cut(s) 122, 244, 430, 433, 475, 648, 711, 1433
MfeI CAATTG 1 cut(s) 171
MflI RGATCY 3 cut(s) 4, 676, 1390
MhlI GDGCHC 5 cut(s) 69, 451, 585, 1072, 1307
MluCI AATT 5 cut(s) 114, 171, 1006, 1033, 1355
MlyI GAGTC 1 cut(s) 418
MmeI TCCRAC 1 cut(s) 720
Mph1103I ATGCAT 1 cut(s) 483
MseI TTAA 1 cut(s) 360
MslI CAYNNNNRTG 2 cut(s) 41, 1310
MspA1I CMGCKG 1 cut(s) 877
MspI CCGG 2 cut(s) 83, 1296
MspR9I CCNGG 3 cut(s) 84, 407, 647
MunI CAATTG 1 cut(s) 171
MvaI CCWGG 2 cut(s) 407, 647
MwoI GCNNNNNNNGC 1 cut(s) 373
NciI CCSGG 1 cut(s) 84
NdeII GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1384, 1390
NlaIII CATG 8 cut(s) 40, 481, 485, 580, 641, 706, 791, 1124
NlaIV GGNNCC 3 cut(s) 6, 678, 1084
NmuCI GTSAC 3 cut(s) 766, 902, 1115
NsiI ATGCAT 1 cut(s) 483
NspV TTCGAA 2 cut(s) 159, 1093
PagI TCATGA 2 cut(s) 637, 702
PcsI WCGNNNNNNNCGW 1 cut(s) 299
PfeI GAWTC 3 cut(s) 295, 308, 792
PfoI TCCNGGA 1 cut(s) 645
PkrI GCNGC 9 cut(s) 155, 166, 169, 369, 372, 375, 535, 842, 879
PleI GAGTC 1 cut(s) 418
PpsI GAGTC 1 cut(s) 418
PshAI GACNNNNGTC 1 cut(s) 900
PsiI TTATAA 1 cut(s) 119
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 2 cut(s) 405, 645
PspGI CCWGG 2 cut(s) 405, 645
PspN4I GGNNCC 3 cut(s) 6, 678, 1084
PspPI GGNCC 1 cut(s) 1083
PsuI RGATCY 3 cut(s) 4, 676, 1390
PvuII CAGCTG 1 cut(s) 877
RsaI GTAC 5 cut(s) 248, 317, 1102, 1108, 1366
RsaNI GTAC 5 cut(s) 247, 316, 1101, 1107, 1365
RseI CAYNNNNRTG 2 cut(s) 41, 1310
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 1 cut(s) 360
SatI GCNGC 9 cut(s) 154, 165, 168, 368, 371, 374, 534, 841, 878
Sau3AI GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1384, 1390
Sau96I GGNCC 1 cut(s) 1083
ScaI AGTACT 2 cut(s) 1102, 1108
SchI GAGTC 1 cut(s) 418
ScrFI CCNGG 3 cut(s) 84, 407, 647
SduI GDGCHC 5 cut(s) 69, 451, 585, 1072, 1307
SfaNI GCATC 3 cut(s) 392, 889, 982
SfcI CTRYAG 1 cut(s) 436
SfuI TTCGAA 2 cut(s) 159, 1093
SinI GGWCC 1 cut(s) 1083
SmiMI CAYNNNNRTG 2 cut(s) 41, 1310
SmlI CTYRAG 3 cut(s) 68, 854, 1071
SmoI CTYRAG 3 cut(s) 68, 854, 1071
SpeI ACTAGT 2 cut(s) 1103, 1109
Sse9I AATT 5 cut(s) 114, 171, 1006, 1033, 1355
SsiI CCGC 1 cut(s) 154
SspI AATATT 2 cut(s) 628, 1192
SspMI CTAG 5 cut(s) 123, 606, 996, 1104, 1110
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 3 cut(s) 82, 405, 645
StyI CCWWGG 2 cut(s) 203, 528
TaaI ACNGT 3 cut(s) 766, 806, 902
TaqI TCGA 6 cut(s) 11, 159, 298, 1093, 1248, 1259
TasI AATT 5 cut(s) 114, 171, 1006, 1033, 1355
TatI WGTACW 2 cut(s) 1100, 1106
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 3 cut(s) 295, 308, 792
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TscAI CASTG 2 cut(s) 771, 1244
TseFI GTSAC 3 cut(s) 766, 902, 1115
TseI GCWGC 8 cut(s) 164, 167, 367, 370, 373, 533, 840, 877
Tsp45I GTSAC 3 cut(s) 766, 902, 1115
TspDTI ATGAA 7 cut(s) 86, 123, 431, 498, 626, 813, 1328
TspGWI ACGGA 4 cut(s) 119, 320, 1075, 1322
TspRI CASTG 2 cut(s) 771, 1244
VpaK11BI GGWCC 1 cut(s) 1083
XagI CCTNNNNNAGG 1 cut(s) 527
XspI CTAG 5 cut(s) 123, 606, 996, 1104, 1110
ZrmI AGTACT 2 cut(s) 1102, 1108
Zsp2I ATGCAT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.