RLG00000022457
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0011692
Physical Location & Seq
Forward (+)
616670 .. 618486
1817 bp
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UTR
Exon/CDS
Intron
RLM00000022457

Sequence Viewer

Length: 1416 bp
ATGGAAGAAGAAGCTGTCGTGGTGAACCAAGGAGGAGGAGGAGATGATGAGCTGATGGCCTTGCCTCCGGGATTTCGTTTCCATCCCACCGACGAGGAAATCATAACTTGCTATCTCACGGAGAAGGTGGTCAACAGCAACTTCACCGCAACTGCAATCGGTGAAGCTGATTTGAACAAGTGTGAACCTTGGGATTTACCCAACAAGGCAAAGATGGGGGAGAAGGAGTGGTACTTCTTCTGCCAGAGAGACAGGAAGTATCCCACAGGCATGAGAACGAATCGAGCAACGCAATCTGGTTACTGGAAAGCCACCGGAAAAGATAAAGAGATCTACAAGCGCAAAACCAACAACAGTAACTGCCTTGTCGGGATGAAGAAGACTCTTGTGTTTTACACCGGAAGAGCTCCAAAGGGTGAGAAGACCAACTGGGTCATGCACGAATACAGACTCGACGGCAAATTCACACATTACAATCTTTCTAAGCCTTCAAAGGATGAATGGGTTGTGTGCAGGGTTTTCCACAAGAACATGGGGCTTAAAAGGACTAGCAGTCCATTTTCTCCGACTGATCAAGGCGCTGGTGGCCAGCTGCTAAGGCTCAACTCTTTTGGGGATGATTTCTTGGATTCTTCTTCTTCACTCCCACCTCTCATGGATCCTACTCCTTCCTACGACAGGCCTGCAGGCTCCTATGGCGGCTACAGCCTCGACTATGGAGAGCACGACGACAACGATTCCAAAGGAAAAATCACAGCAGCACCATTATCAGTACCAGCAGCCAATAAAACAACACCAGCAGATATGCAATACTACTTTCAAGTTGGCTCTTCCACTCGTCAGATCAACTGCTTTCAGCATCCCAACGCTGCTGCTGCTGCCGCTAGCTACCAAATGAGCAACCCAATGGCGACCCCCACCATGTTATACCCTCAGATTCATCATCATCACCAGTTGATTCCGAACCCTTCCTTCTCTTTCCAGCAAAATAATAATCCTGATTATTACTTCCAACAAGAGATAAGGGGCCCGAGGACTTCATTCCCAAACAATCAAGGCTCCTCCTTCGACTTTCAAAGCAGCAAACTTGATCATCAGGCCATTTTAAGAGCAATGGCTGCAGCTAAAAACAGCAACCACCAGATTGAAACATCAGTAACAACAGCAGGCTCAGGGGGCTTAGACAGTAGACAGTGCAAGGTGGAGCAGTATTCATCCAACAACCAGTCTATGTTTAGCGTCTCGCAAGACACTGGATTGAGCACCGCTGACATGAACACCACCACCGAGATATCATCATCAGTTGTCTCAAAGCAAGAAATCGAGGTCGGAAGCAGCAACACATTATATGAAGATCATCTTCAAAGTCCATCCGTTGTCCCTATTTCGGACATTGAAGGCTTGTGGGACTATTGA

Protein Analysis

472

Amino Acids

52.47

Weight (kDa)

5.83

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 22 - 151 2e-49 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011692)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04060 AT5G18270 AT5G18270
fragaria_vesca FvH4_1g27900
malus_domestica MD12G1001200.v1.1 MD14G1001200.v1.1
prunus_persica Prupe.7G001400_v2.0.a1
pyrus_communis pycom12g00110 pycom49g00070
rosa_chinensis RchiOBHm_Chr3g0497471
rosa_laevigata RLG00000022457
rosa_multiflora Rmu_sc0008086.1_g000018
rosa_roxburghii Rroxscaffold_6G00387990
rosa_rugosa Rorug03G0293100
rosa_samantha Rh3AG336800 Rh3BG375100 Rh3CG371100 Rh3DG371800
rosa_wichuraiana Rw3G029600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1189
AciI CCGC 4 cut(s) 147, 699, 882, 1266
AclWI GGATC 2 cut(s) 653, 666
AcoI YGGCCR 1 cut(s) 586
AcsI RAATTY 1 cut(s) 461
AfaI GTAC 2 cut(s) 233, 774
AfiI CCNNNNNNNGG 2 cut(s) 678, 1387
AgsI TTSAA 7 cut(s) 175, 492, 821, 1076, 1148, 1364, 1397
AhdI GACNNNNNGTC 1 cut(s) 552
AluBI AGCT 7 cut(s) 14, 52, 167, 407, 592, 888, 1124
AluI AGCT 7 cut(s) 14, 52, 167, 407, 592, 888, 1124
Alw21I GWGCWC 3 cut(s) 409, 726, 1265
Alw26I GTCTC 3 cut(s) 243, 1246, 1312
AlwI GGATC 2 cut(s) 653, 666
AlwNI CAGNNNCTG 1 cut(s) 360
Ama87I CYCGRG 1 cut(s) 1030
AoxI GGCC 5 cut(s) 57, 586, 680, 1027, 1098
ApaI GGGCCC 1 cut(s) 1031
ApoI RAATTY 1 cut(s) 461
AspLEI GCGC 2 cut(s) 342, 581
AspS9I GGNCC 2 cut(s) 1027, 1028
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 5 cut(s) 34, 136, 173, 428, 941
AsuNHI GCTAGC 1 cut(s) 884
AvaI CYCGRG 1 cut(s) 1030
BaeGI GKGCMC 1 cut(s) 1031
BalI TGGCCA 1 cut(s) 588
BamHI GGATCC 1 cut(s) 658
BanII GRGCYC 2 cut(s) 409, 1031
BarI GAAGNNNNNNTAC 2 cut(s) 215, 247
BbsI GAAGAC 2 cut(s) 386, 428
Bbv12I GWGCWC 3 cut(s) 409, 726, 1265
BccI CCATC 4 cut(s) 49, 90, 208, 1378
BceAI ACGGC 1 cut(s) 472
BcgI CGANNNNNNTGC 2 cut(s) 665, 699
BciVI GTATCC 1 cut(s) 270
BclI TGATCA 2 cut(s) 571, 1090
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 3 cut(s) 243, 1246, 1312
BfaI CTAG 2 cut(s) 549, 885
BfmI CTRYAG 3 cut(s) 684, 703, 1119
BfoI RGCGCY 1 cut(s) 582
BfuI GTATCC 1 cut(s) 270
BglII AGATCT 1 cut(s) 330
Bme1390I CCNGG 1 cut(s) 69
BmeRI GACNNNNNGTC 1 cut(s) 552
BmeT110I CYCGRG 1 cut(s) 1030
BmgT120I GGNCC 2 cut(s) 1027, 1028
BmiI GGNNCC 5 cut(s) 660, 691, 1028, 1029, 1060
BmrFI CCNGG 1 cut(s) 69
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 1 cut(s) 868
BmtI GCTAGC 1 cut(s) 888
BmuI ACTGGG 1 cut(s) 439
BpiI GAAGAC 2 cut(s) 386, 428
Bpu10I CCTNAGC 2 cut(s) 596, 1171
BpuMI CCSGG 1 cut(s) 69
BsaJI CCNNGG 3 cut(s) 28, 188, 1031
BsaWI WCCGGW 2 cut(s) 314, 398
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 2 cut(s) 678, 1387
Bse1I ACTGG 5 cut(s) 308, 434, 952, 1225, 1258
Bse3DI GCAATG 1 cut(s) 1119
BseDI CCNNGG 3 cut(s) 28, 188, 1031
BseGI GGATG 7 cut(s) 82, 378, 502, 622, 859, 1214, 1370
BseLI CCNNNNNNNGG 2 cut(s) 678, 1387
BseMI GCAATG 1 cut(s) 1119
BseMII CTCAG 2 cut(s) 947, 1185
BseNI ACTGG 5 cut(s) 308, 434, 952, 1225, 1258
BseRI GAGGAG 4 cut(s) 48, 51, 54, 1051
BseSI GKGCMC 1 cut(s) 1031
BsgI GTGCAG 1 cut(s) 532
BshFI GGCC 5 cut(s) 59, 588, 682, 1029, 1100
BsiHKAI GWGCWC 3 cut(s) 409, 726, 1265
BsiHKCI CYCGRG 1 cut(s) 1030
BsiSI CCGG 3 cut(s) 68, 315, 399
BslFI GGGAC 1 cut(s) 1364
BslI CCNNNNNNNGG 2 cut(s) 678, 1387
BsmAI GTCTC 3 cut(s) 243, 1246, 1312
BsmBI CGTCTC 1 cut(s) 1246
BsmFI GGGAC 1 cut(s) 1364
BsnI GGCC 5 cut(s) 59, 588, 682, 1029, 1100
BsoBI CYCGRG 1 cut(s) 1030
Bsp120I GGGCCC 1 cut(s) 1027
Bsp1286I GDGCHC 4 cut(s) 409, 726, 1031, 1265
Bsp143I GATC 6 cut(s) 330, 571, 658, 843, 1090, 1354
BspACI CCGC 4 cut(s) 147, 699, 882, 1266
BspANI GGCC 5 cut(s) 59, 588, 682, 1029, 1100
BspCNI CTCAG 2 cut(s) 946, 1184
BspLI GGNNCC 5 cut(s) 660, 691, 1028, 1029, 1060
BspMAI CTGCAG 2 cut(s) 688, 1123
BspOI GCTAGC 1 cut(s) 888
BspPI GGATC 2 cut(s) 653, 666
BspQI GCTCTTC 2 cut(s) 397, 835
BsrDI GCAATG 1 cut(s) 1119
BsrI ACTGG 5 cut(s) 308, 434, 952, 1225, 1258
BssECI CCNNGG 3 cut(s) 28, 188, 1031
BssMI GATC 6 cut(s) 330, 571, 658, 843, 1090, 1354
BssT1I CCWWGG 2 cut(s) 28, 188
Bst4CI ACNGT 3 cut(s) 356, 1187, 1194
Bst6I CTCTTC 2 cut(s) 397, 835
BstAPI GCANNNNNTGC 1 cut(s) 1118
BstC8I GCNNGC 5 cut(s) 590, 684, 688, 886, 1168
BstDEI CTNAG 5 cut(s) 483, 596, 933, 1171, 1180
BstENI CCTNNNNNAGG 1 cut(s) 676
BstF5I GGATG 7 cut(s) 82, 378, 502, 622, 859, 1214, 1370
BstH2I RGCGCY 1 cut(s) 582
BstHHI GCGC 2 cut(s) 342, 581
BstKTI GATC 6 cut(s) 333, 574, 661, 846, 1093, 1357
BstMAI GTCTC 3 cut(s) 243, 1246, 1312
BstMBI GATC 6 cut(s) 330, 571, 658, 843, 1090, 1354
BstMWI GCNNNNNNNGC 9 cut(s) 585, 598, 696, 705, 875, 878, 881, 1118, 1176
BstSCI CCNGG 1 cut(s) 67
BstSFI CTRYAG 3 cut(s) 684, 703, 1119
BstSLI GKGCMC 1 cut(s) 1031
BstV2I GAAGAC 2 cut(s) 386, 428
BstX2I RGATCY 2 cut(s) 330, 658
BstYI RGATCY 2 cut(s) 330, 658
BsuI GTATCC 1 cut(s) 270
BsuRI GGCC 5 cut(s) 59, 588, 682, 1029, 1100
BtsCI GGATG 7 cut(s) 82, 378, 502, 622, 859, 1214, 1370
BtsIMutI CAGTG 2 cut(s) 1199, 1251
Cac8I GCNNGC 5 cut(s) 590, 684, 688, 886, 1168
CaiI CAGNNNCTG 1 cut(s) 360
CfoI GCGC 2 cut(s) 342, 581
Cfr13I GGNCC 2 cut(s) 1027, 1028
CseI GACGC 1 cut(s) 1228
Csp6I GTAC 2 cut(s) 232, 773
CviAII CATG 6 cut(s) 271, 436, 532, 655, 922, 1273
CviQI GTAC 2 cut(s) 232, 773
DdeI CTNAG 5 cut(s) 483, 596, 933, 1171, 1180
DpnI GATC 6 cut(s) 332, 573, 660, 845, 1092, 1356
DpnII GATC 6 cut(s) 330, 571, 658, 843, 1090, 1354
DriI GACNNNNNGTC 1 cut(s) 552
EaeI YGGCCR 1 cut(s) 586
Eam1104I CTCTTC 2 cut(s) 397, 835
Eam1105I GACNNNNNGTC 1 cut(s) 552
EarI CTCTTC 2 cut(s) 397, 835
Ecl136II GAGCTC 1 cut(s) 407
Eco130I CCWWGG 2 cut(s) 28, 188
Eco147I AGGCCT 1 cut(s) 682
Eco24I GRGCYC 2 cut(s) 409, 1031
Eco32I GATATC 1 cut(s) 1293
Eco53kI GAGCTC 1 cut(s) 407
Eco88I CYCGRG 1 cut(s) 1030
EcoICRI GAGCTC 1 cut(s) 407
EcoNI CCTNNNNNAGG 1 cut(s) 676
EcoO109I RGGNCCY 1 cut(s) 1027
EcoRV GATATC 1 cut(s) 1293
EcoT14I CCWWGG 2 cut(s) 28, 188
EcoT38I GRGCYC 2 cut(s) 409, 1031
ErhI CCWWGG 2 cut(s) 28, 188
Esp3I CGTCTC 1 cut(s) 1246
FaeI CATG 6 cut(s) 274, 439, 535, 658, 925, 1276
FalI AAGNNNNNCTT 2 cut(s) 1344, 1376
FaqI GGGAC 1 cut(s) 1364
FatI CATG 6 cut(s) 270, 435, 531, 654, 921, 1272
FbaI TGATCA 2 cut(s) 571, 1090
FblI GTMKAC 1 cut(s) 1189
FokI GGATG 7 cut(s) 69, 385, 509, 629, 846, 1201, 1357
FriOI GRGCYC 2 cut(s) 409, 1031
FspBI CTAG 2 cut(s) 549, 885
GlaI GCGC 2 cut(s) 341, 580
HaeII RGCGCY 1 cut(s) 582
HaeIII GGCC 5 cut(s) 59, 588, 682, 1029, 1100
HapII CCGG 3 cut(s) 68, 315, 399
HgaI GACGC 1 cut(s) 1228
HhaI GCGC 2 cut(s) 342, 581
Hin1II CATG 6 cut(s) 274, 439, 535, 658, 925, 1276
Hin6I GCGC 2 cut(s) 340, 579
HinP1I GCGC 2 cut(s) 340, 579
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HinfI GANTC 7 cut(s) 280, 382, 450, 629, 737, 937, 958
HpaII CCGG 3 cut(s) 68, 315, 399
HphI GGTGA 5 cut(s) 34, 136, 173, 428, 941
Hpy166II GTNNAC 4 cut(s) 25, 133, 185, 1190
Hpy188I TCNGA 6 cut(s) 567, 843, 936, 963, 1331, 1390
Hpy188III TCNNGA 2 cut(s) 370, 998
Hpy8I GTNNAC 4 cut(s) 25, 133, 185, 1190
Hpy99I CGWCG 3 cut(s) 95, 458, 731
HpyAV CCTTC 8 cut(s) 118, 217, 498, 678, 978, 982, 1075, 1391
HpyCH4III ACNGT 3 cut(s) 356, 1187, 1194
HpyCH4V TGCA 7 cut(s) 155, 439, 513, 686, 808, 1121, 1197
HpyF10VI GCNNNNNNNGC 9 cut(s) 585, 598, 696, 705, 875, 878, 881, 1118, 1176
HpyF3I CTNAG 5 cut(s) 483, 596, 933, 1171, 1180
Hsp92II CATG 6 cut(s) 274, 439, 535, 658, 925, 1276
HspAI GCGC 2 cut(s) 340, 579
Ksp22I TGATCA 2 cut(s) 571, 1090
Kzo9I GATC 6 cut(s) 330, 571, 658, 843, 1090, 1354
LguI GCTCTTC 2 cut(s) 397, 835
LmnI GCTCC 4 cut(s) 412, 695, 1064, 1204
LweI GCATC 1 cut(s) 868
MaeI CTAG 2 cut(s) 549, 885
MaeIII GTNAC 3 cut(s) 299, 356, 1156
MalI GATC 6 cut(s) 332, 573, 660, 845, 1092, 1356
MboI GATC 6 cut(s) 330, 571, 658, 843, 1090, 1354
MflI RGATCY 2 cut(s) 330, 658
MhlI GDGCHC 4 cut(s) 409, 726, 1031, 1265
MlsI TGGCCA 1 cut(s) 588
MluCI AATT 1 cut(s) 461
MluNI TGGCCA 1 cut(s) 588
MlyI GAGTC 2 cut(s) 376, 444
MmeI TCCRAC 4 cut(s) 590, 1036, 1242, 1309
Mox20I TGGCCA 1 cut(s) 588
MscI TGGCCA 1 cut(s) 588
MseI TTAA 2 cut(s) 540, 1106
MslI CAYNNNNRTG 1 cut(s) 269
Msp20I TGGCCA 1 cut(s) 588
MspA1I CMGCKG 2 cut(s) 592, 1268
MspI CCGG 3 cut(s) 68, 315, 399
MspR9I CCNGG 1 cut(s) 69
MwoI GCNNNNNNNGC 9 cut(s) 585, 598, 696, 705, 875, 878, 881, 1118, 1176
NciI CCSGG 1 cut(s) 69
NdeII GATC 6 cut(s) 330, 571, 658, 843, 1090, 1354
NheI GCTAGC 1 cut(s) 884
NlaIII CATG 6 cut(s) 274, 439, 535, 658, 925, 1276
NlaIV GGNNCC 5 cut(s) 660, 691, 1028, 1029, 1060
PceI AGGCCT 1 cut(s) 682
PciSI GCTCTTC 2 cut(s) 397, 835
PcsI WCGNNNNNNNCGW 1 cut(s) 732
PfeI GAWTC 5 cut(s) 280, 629, 737, 937, 958
PfoI TCCNGGA 1 cut(s) 67
PleI GAGTC 2 cut(s) 376, 444
PpsI GAGTC 2 cut(s) 376, 444
Psp124BI GAGCTC 1 cut(s) 409
PspN4I GGNNCC 5 cut(s) 660, 691, 1028, 1029, 1060
PspOMI GGGCCC 1 cut(s) 1027
PspPI GGNCC 2 cut(s) 1027, 1028
PstI CTGCAG 2 cut(s) 688, 1123
PstNI CAGNNNCTG 1 cut(s) 360
PsuI RGATCY 2 cut(s) 330, 658
PvuII CAGCTG 1 cut(s) 592
RsaI GTAC 2 cut(s) 233, 774
RsaNI GTAC 2 cut(s) 232, 773
RseI CAYNNNNRTG 1 cut(s) 269
SacI GAGCTC 1 cut(s) 409
SapI GCTCTTC 2 cut(s) 397, 835
SaqAI TTAA 2 cut(s) 540, 1106
Sau3AI GATC 6 cut(s) 330, 571, 658, 843, 1090, 1354
Sau96I GGNCC 2 cut(s) 1027, 1028
SbfI CCTGCAGG 1 cut(s) 688
SchI GAGTC 2 cut(s) 376, 444
ScrFI CCNGG 1 cut(s) 69
SdaI CCTGCAGG 1 cut(s) 688
SduI GDGCHC 4 cut(s) 409, 726, 1031, 1265
SfaNI GCATC 1 cut(s) 868
SfcI CTRYAG 3 cut(s) 684, 703, 1119
SmiMI CAYNNNNRTG 1 cut(s) 269
Sse8387I CCTGCAGG 1 cut(s) 688
Sse9I AATT 1 cut(s) 461
SseBI AGGCCT 1 cut(s) 682
SsiI CCGC 4 cut(s) 147, 699, 882, 1266
SspMI CTAG 2 cut(s) 549, 885
SstI GAGCTC 1 cut(s) 409
StuI AGGCCT 1 cut(s) 682
StyD4I CCNGG 1 cut(s) 67
StyI CCWWGG 2 cut(s) 28, 188
TaaI ACNGT 3 cut(s) 356, 1187, 1194
TaqI TCGA 5 cut(s) 283, 453, 711, 1068, 1323
TasI AATT 1 cut(s) 461
TauI GCSGC 2 cut(s) 702, 884
TfiI GAWTC 5 cut(s) 280, 629, 737, 937, 958
Tru1I TTAA 2 cut(s) 540, 1106
Tru9I TTAA 2 cut(s) 540, 1106
TscAI CASTG 2 cut(s) 1199, 1258
TspDTI ATGAA 7 cut(s) 389, 513, 929, 1029, 1203, 1289, 1365
TspGWI ACGGA 2 cut(s) 134, 1363
TspRI CASTG 2 cut(s) 1199, 1258
XagI CCTNNNNNAGG 1 cut(s) 676
XapI RAATTY 1 cut(s) 461
XmiI GTMKAC 1 cut(s) 1189
XspI CTAG 2 cut(s) 549, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.