MD14G1001200.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011692
Physical Location & Seq
Forward (+)
256059 .. 258347
2289 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1001200.v1.1.491

Sequence Viewer

Length: 1449 bp
ATGGATCCGTCGATTGAAGCACAAACAGAAACAGGCATGGTGGTGAATAGAGGATTATCAGACGAGCTCAAGGGGTTGCCTCCGGGTTTTCGGTTTCATCCCACGGATGAAGAAATCATAACTAGCTATCTCATAAAGAAGGTCATCAACAGCAGCTTCGAAGCTGTTGCAATCGGCGAAGCTGATTTGAACAAGTGCGAACCTTGGGATTTGCCTAAAGAAGCAAAGATGGGAGAGAAGGAGTGGTACTTCTTTTGCCAGAGAGACAGGAAGTATCCGACGGGGATGAGGACGAATCGTGCGACGGAATCAGGGTACTGGAAGGCCACAGGAAAGGATAAAGAGATATTCAATAAGGTTAAAAAGCAGCAACAGCAGGGCGGGAAAGGTGCAGGAGGAGGATGCCTGGTTGGGATGAAGAAGACTCTTGTGTTCTACAGAGGGAGAGCCCCTAAAGGAGAGAAGACCAACTGGGTCATGCATGAATACAGGCTTGATGGCAAAATCCCACTCTACAATAACCTCTCCAAGGCTGCAAAGGATGAATGGGTTGTGTGCAGAGTTTTCCACAAGAACATGGGGCTCAAAAGAACCCTTACCCCAACTCGGATGAACTCTTTTGGGAATATTGGGGATCACGATCTCTTGGATTGTTCTTCCCTCCCACCTCTTATGGATCCTCCTCTTAATACTTCCAACATCATGACCAACAAACATTCTTCATGCTATGGAGACAATGACTTCAAGCCCGATTCCATTATCACAGCGCCACACCCATCGGATTATCGTGGAATCAACACTAACAGTTACCTCAACTACGTTTCACTGGGGGGAGGCGGCCGCGGTGTTCAAGGCCTCAAGCAGAGTTGCAACTTTCAGCTGCAGCCTAACAATAACTACAACAGTTACCAAGCCATTAGTCTCAGTAACCCTTCCAACATGTTATATCCTCCACCTCTTCATCATAATCATCAGCATCATCACCAGTTTCAAAATCCTTCTAGTTTCCCAAATTTCCAGCGAAATCAGATGATGAGCGATTCTGATTATTTTCAACAAGGGATGATGAGGGGTACTCAAGGGCAAGGGTCGGTGTTCCAAACAAGTACTATTACTAGTAGTGACCGTGATGACGATGATCAGGCCATTCTAAAAGCAATAGCTGCAGACAACAACAACAACAACAAGAGGAAGAATAATAATGAAACATCATCGTCTGGCTTAGGAGGAGGAGGCTTAGACAGGCACTGCAAGGTCGAGCAGTTTTCGTCCAACCAGTCTATGTTTAGCCTCTCCCAAGACACCGGATTGAGCACCGACATGAACACTACCGAGATATCATCATCATTTATTTCAAAGCAAGAATTGGGCAGCACCAACAACTCATCCTATGATCAAGATCCATCTGTTGTCCCCCTTTCCGACATTGAAGGCTTGTGGGGCTTTTGA

Protein Analysis

483

Amino Acids

53.79

Weight (kDa)

6.89

Isoelectric Point (pI)

36.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 27 - 165 1.1e-47 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011692)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04060 AT5G18270 AT5G18270
fragaria_vesca FvH4_1g27900
malus_domestica MD12G1001200.v1.1 MD14G1001200.v1.1
prunus_persica Prupe.7G001400_v2.0.a1
pyrus_communis pycom12g00110 pycom49g00070
rosa_chinensis RchiOBHm_Chr3g0497471
rosa_laevigata RLG00000022457
rosa_multiflora Rmu_sc0008086.1_g000018
rosa_roxburghii Rroxscaffold_6G00387990
rosa_rugosa Rorug03G0293100
rosa_samantha Rh3AG336800 Rh3BG375100 Rh3CG371100 Rh3DG371800
rosa_wichuraiana Rw3G029600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 843
AciI CCGC 4 cut(s) 381, 837, 841, 843
AclWI GGATC 5 cut(s) 12, 642, 671, 684, 1394
AcoI YGGCCR 1 cut(s) 838
AcsI RAATTY 1 cut(s) 1012
AfaI GTAC 4 cut(s) 248, 317, 1075, 1108
AfiI CCNNNNNNNGG 2 cut(s) 529, 606
AflIII ACRYGT 1 cut(s) 939
AgsI TTSAA 9 cut(s) 17, 190, 352, 745, 851, 992, 1055, 1356, 1430
AhlI ACTAGT 1 cut(s) 1115
AjnI CCWGG 1 cut(s) 405
AluBI AGCT 7 cut(s) 67, 126, 156, 164, 182, 880, 1163
AluI AGCT 7 cut(s) 67, 126, 156, 164, 182, 880, 1163
Alw21I GWGCWC 2 cut(s) 69, 1316
Alw26I GTCTC 3 cut(s) 258, 726, 926
AlwI GGATC 5 cut(s) 12, 642, 671, 684, 1394
AlwNI CAGNNNCTG 1 cut(s) 1248
AoxI GGCC 4 cut(s) 324, 838, 853, 1143
ApeKI GCWGC 7 cut(s) 153, 367, 533, 880, 883, 1163, 1371
ApoI RAATTY 1 cut(s) 1012
AspLEI GCGC 1 cut(s) 769
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 2 cut(s) 55, 974
AsuII TTCGAA 1 cut(s) 159
BamHI GGATCC 2 cut(s) 4, 676
BanII GRGCYC 3 cut(s) 69, 451, 585
BarI GAAGNNNNNNTAC 2 cut(s) 230, 262
BbsI GAAGAC 2 cut(s) 428, 470
Bbv12I GWGCWC 2 cut(s) 69, 1316
BbvI GCAGC 7 cut(s) 165, 379, 520, 867, 895, 1150, 1383
BccI CCATC 4 cut(s) 223, 491, 784, 1411
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BciT130I CCWGG 1 cut(s) 407
BciVI GTATCC 1 cut(s) 285
BclI TGATCA 2 cut(s) 1138, 1393
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 3 cut(s) 258, 726, 926
BcuI ACTAGT 1 cut(s) 1115
BfaI CTAG 3 cut(s) 123, 1002, 1116
BfmI CTRYAG 3 cut(s) 436, 881, 1164
BfoI RGCGCY 1 cut(s) 770
BfuI GTATCC 1 cut(s) 285
BisI GCNGC 9 cut(s) 154, 368, 534, 838, 841, 881, 884, 1164, 1372
BlsI GCNGC 9 cut(s) 155, 369, 535, 839, 842, 882, 885, 1165, 1373
BmcAI AGTACT 1 cut(s) 1108
Bme1390I CCNGG 2 cut(s) 84, 407
BmiI GGNNCC 2 cut(s) 6, 678
BmrFI CCNGG 2 cut(s) 84, 407
BmrI ACTGGG 2 cut(s) 481, 836
BmsI GCATC 2 cut(s) 392, 985
BmuI ACTGGG 2 cut(s) 481, 836
BpiI GAAGAC 2 cut(s) 428, 470
BplI GAGNNNNNCTC 2 cut(s) 1060, 1092
Bpu10I CCTNAGC 1 cut(s) 1222
Bpu14I TTCGAA 1 cut(s) 159
BpuEI CTTGAG 3 cut(s) 53, 842, 1062
BpuMI CCSGG 1 cut(s) 84
BsaBI GATNNNNATC 2 cut(s) 639, 1398
BsaJI CCNNGG 4 cut(s) 102, 203, 528, 841
BsaWI WCCGGW 1 cut(s) 1304
Bsc4I CCNNNNNNNGG 2 cut(s) 529, 606
Bse1I ACTGG 5 cut(s) 323, 476, 831, 985, 1276
Bse8I GATNNNNATC 2 cut(s) 639, 1398
BseBI CCWGG 1 cut(s) 407
BseDI CCNNGG 4 cut(s) 102, 203, 528, 841
BseGI GGATG 9 cut(s) 97, 112, 291, 407, 420, 547, 615, 1068, 1385
BseJI GATNNNNATC 2 cut(s) 639, 1398
BseLI CCNNNNNNNGG 2 cut(s) 529, 606
BseMII CTCAG 1 cut(s) 937
BseNI ACTGG 5 cut(s) 323, 476, 831, 985, 1276
BseRI GAGGAG 4 cut(s) 411, 672, 1242, 1245
BseX3I CGGCCG 1 cut(s) 838
BseXI GCAGC 7 cut(s) 165, 379, 520, 867, 895, 1150, 1383
BsgI GTGCAG 2 cut(s) 411, 577
Bsh1236I CGCG 1 cut(s) 843
Bsh1285I CGRYCG 1 cut(s) 841
BshFI GGCC 4 cut(s) 326, 840, 855, 1145
BsiEI CGRYCG 1 cut(s) 841
BsiHKAI GWGCWC 2 cut(s) 69, 1316
BsiSI CCGG 2 cut(s) 83, 1305
BslFI GGGAC 1 cut(s) 1397
BslI CCNNNNNNNGG 2 cut(s) 529, 606
BsmAI GTCTC 3 cut(s) 258, 726, 926
BsmFI GGGAC 1 cut(s) 1397
BsnI GGCC 4 cut(s) 326, 840, 855, 1145
Bsp119I TTCGAA 1 cut(s) 159
Bsp1286I GDGCHC 4 cut(s) 69, 451, 585, 1316
Bsp143I GATC 7 cut(s) 4, 634, 640, 676, 1138, 1393, 1399
BspACI CCGC 4 cut(s) 381, 837, 841, 843
BspANI GGCC 4 cut(s) 326, 840, 855, 1145
BspCNI CTCAG 1 cut(s) 936
BspFNI CGCG 1 cut(s) 843
BspHI TCATGA 1 cut(s) 702
BspLI GGNNCC 2 cut(s) 6, 678
BspMAI CTGCAG 2 cut(s) 885, 1168
BspPI GGATC 5 cut(s) 12, 642, 671, 684, 1394
BspT104I TTCGAA 1 cut(s) 159
BsrI ACTGG 5 cut(s) 323, 476, 831, 985, 1276
BssECI CCNNGG 4 cut(s) 102, 203, 528, 841
BssMI GATC 7 cut(s) 4, 634, 640, 676, 1138, 1393, 1399
BssT1I CCWWGG 2 cut(s) 203, 528
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 3 cut(s) 806, 905, 1127
Bst6I CTCTTC 1 cut(s) 963
BstAPI GCANNNNNTGC 1 cut(s) 1163
BstBI TTCGAA 1 cut(s) 159
BstDEI CTNAG 3 cut(s) 923, 1222, 1237
BstDSI CCRYGG 2 cut(s) 102, 841
BstENI CCTNNNNNAGG 1 cut(s) 527
BstF5I GGATG 9 cut(s) 97, 112, 291, 407, 420, 547, 615, 1068, 1385
BstFNI CGCG 1 cut(s) 843
BstH2I RGCGCY 1 cut(s) 770
BstHHI GCGC 1 cut(s) 769
BstKTI GATC 7 cut(s) 7, 637, 643, 679, 1141, 1396, 1402
BstMAI GTCTC 3 cut(s) 258, 726, 926
BstMBI GATC 7 cut(s) 4, 634, 640, 676, 1138, 1393, 1399
BstMCI CGRYCG 1 cut(s) 841
BstMWI GCNNNNNNNGC 3 cut(s) 373, 1163, 1440
BstNI CCWGG 1 cut(s) 407
BstNSI RCATGY 1 cut(s) 943
BstSCI CCNGG 2 cut(s) 82, 405
BstSFI CTRYAG 3 cut(s) 436, 881, 1164
BstUI CGCG 1 cut(s) 843
BstV1I GCAGC 7 cut(s) 165, 379, 520, 867, 895, 1150, 1383
BstV2I GAAGAC 2 cut(s) 428, 470
BstX2I RGATCY 3 cut(s) 4, 676, 1399
BstYI RGATCY 3 cut(s) 4, 676, 1399
BstZI CGGCCG 1 cut(s) 838
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 4 cut(s) 326, 840, 855, 1145
BtgI CCRYGG 2 cut(s) 102, 841
BtsCI GGATG 9 cut(s) 97, 112, 291, 407, 420, 547, 615, 1068, 1385
BtsI GCAGTG 1 cut(s) 1246
BtsIMutI CAGTG 2 cut(s) 824, 1246
CaiI CAGNNNCTG 1 cut(s) 1248
CciI TCATGA 1 cut(s) 702
CciNI GCGGCCGC 1 cut(s) 838
CfoI GCGC 1 cut(s) 769
Cfr42I CCGCGG 1 cut(s) 844
Csp6I GTAC 4 cut(s) 247, 316, 1074, 1107
CviAII CATG 8 cut(s) 37, 478, 482, 577, 703, 723, 940, 1321
CviQI GTAC 4 cut(s) 247, 316, 1074, 1107
DdeI CTNAG 3 cut(s) 923, 1222, 1237
DpnI GATC 7 cut(s) 6, 636, 642, 678, 1140, 1395, 1401
DpnII GATC 7 cut(s) 4, 634, 640, 676, 1138, 1393, 1399
EaeI YGGCCR 1 cut(s) 838
EagI CGGCCG 1 cut(s) 838
Eam1104I CTCTTC 1 cut(s) 963
EarI CTCTTC 1 cut(s) 963
Ecl136II GAGCTC 1 cut(s) 67
EclXI CGGCCG 1 cut(s) 838
Eco130I CCWWGG 2 cut(s) 203, 528
Eco147I AGGCCT 1 cut(s) 855
Eco24I GRGCYC 3 cut(s) 69, 451, 585
Eco32I GATATC 1 cut(s) 1338
Eco52I CGGCCG 1 cut(s) 838
Eco53kI GAGCTC 1 cut(s) 67
EcoICRI GAGCTC 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 527
EcoRII CCWGG 1 cut(s) 405
EcoRV GATATC 1 cut(s) 1338
EcoT14I CCWWGG 2 cut(s) 203, 528
EcoT22I ATGCAT 1 cut(s) 483
EcoT38I GRGCYC 3 cut(s) 69, 451, 585
ErhI CCWWGG 2 cut(s) 203, 528
FaeI CATG 8 cut(s) 40, 481, 485, 580, 706, 726, 943, 1324
FaqI GGGAC 1 cut(s) 1397
FatI CATG 8 cut(s) 36, 477, 481, 576, 702, 722, 939, 1320
FauI CCCGC 1 cut(s) 374
FbaI TGATCA 2 cut(s) 1138, 1393
Fnu4HI GCNGC 9 cut(s) 154, 368, 534, 838, 841, 881, 884, 1164, 1372
FokI GGATG 9 cut(s) 84, 119, 298, 414, 427, 554, 622, 1075, 1372
FriOI GRGCYC 3 cut(s) 69, 451, 585
Fsp4HI GCNGC 9 cut(s) 154, 368, 534, 838, 841, 881, 884, 1164, 1372
FspBI CTAG 3 cut(s) 123, 1002, 1116
GlaI GCGC 1 cut(s) 768
GluI GCNGC 9 cut(s) 154, 368, 534, 838, 841, 881, 884, 1164, 1372
HaeII RGCGCY 1 cut(s) 770
HaeIII GGCC 4 cut(s) 326, 840, 855, 1145
HapII CCGG 2 cut(s) 83, 1305
HhaI GCGC 1 cut(s) 769
Hin1II CATG 8 cut(s) 40, 481, 485, 580, 706, 726, 943, 1324
Hin6I GCGC 1 cut(s) 767
HinP1I GCGC 1 cut(s) 767
HinfI GANTC 6 cut(s) 295, 308, 424, 752, 792, 1040
HpaII CCGG 2 cut(s) 83, 1305
HphI GGTGA 2 cut(s) 55, 974
Hpy188I TCNGA 7 cut(s) 61, 279, 609, 781, 1029, 1045, 1423
Hpy188III TCNNGA 3 cut(s) 638, 703, 1397
Hpy99I CGWCG 3 cut(s) 13, 283, 307
HpyAV CCTTC 6 cut(s) 133, 232, 316, 942, 1008, 1424
HpyCH4III ACNGT 3 cut(s) 806, 905, 1127
HpyCH4IV ACGT 1 cut(s) 819
HpyCH4V TGCA 9 cut(s) 170, 392, 481, 536, 558, 870, 883, 1166, 1251
HpyF10VI GCNNNNNNNGC 3 cut(s) 373, 1163, 1440
HpyF3I CTNAG 3 cut(s) 923, 1222, 1237
HpySE526I ACGT 1 cut(s) 819
Hsp92II CATG 8 cut(s) 40, 481, 485, 580, 706, 726, 943, 1324
HspAI GCGC 1 cut(s) 767
Ksp22I TGATCA 2 cut(s) 1138, 1393
KspI CCGCGG 1 cut(s) 844
Kzo9I GATC 7 cut(s) 4, 634, 640, 676, 1138, 1393, 1399
Lsp1109I GCAGC 7 cut(s) 165, 379, 520, 867, 895, 1150, 1383
LweI GCATC 2 cut(s) 392, 985
MaeI CTAG 3 cut(s) 123, 1002, 1116
MaeII ACGT 1 cut(s) 819
MaeIII GTNAC 4 cut(s) 806, 905, 926, 1121
MalI GATC 7 cut(s) 6, 636, 642, 678, 1140, 1395, 1401
MboI GATC 7 cut(s) 4, 634, 640, 676, 1138, 1393, 1399
MboII GAAGA 8 cut(s) 122, 430, 433, 475, 648, 711, 950, 1204
MflI RGATCY 3 cut(s) 4, 676, 1399
MhlI GDGCHC 4 cut(s) 69, 451, 585, 1316
MluCI AATT 2 cut(s) 1012, 1364
MlyI GAGTC 1 cut(s) 418
MmeI TCCRAC 5 cut(s) 302, 720, 960, 1296, 1446
Mph1103I ATGCAT 1 cut(s) 483
MseI TTAA 2 cut(s) 360, 687
MslI CAYNNNNRTG 2 cut(s) 41, 1319
MspA1I CMGCKG 2 cut(s) 843, 880
MspI CCGG 2 cut(s) 83, 1305
MspR9I CCNGG 2 cut(s) 84, 407
MvaI CCWGG 1 cut(s) 407
MvnI CGCG 1 cut(s) 843
MwoI GCNNNNNNNGC 3 cut(s) 373, 1163, 1440
NciI CCSGG 1 cut(s) 84
NdeII GATC 7 cut(s) 4, 634, 640, 676, 1138, 1393, 1399
NlaIII CATG 8 cut(s) 40, 481, 485, 580, 706, 726, 943, 1324
NlaIV GGNNCC 2 cut(s) 6, 678
NmuCI GTSAC 1 cut(s) 1121
NotI GCGGCCGC 1 cut(s) 838
NsiI ATGCAT 1 cut(s) 483
NspI RCATGY 1 cut(s) 943
NspV TTCGAA 1 cut(s) 159
PagI TCATGA 1 cut(s) 702
PceI AGGCCT 1 cut(s) 855
PciI ACATGT 1 cut(s) 939
PcsI WCGNNNNNNNCGW 1 cut(s) 299
PfeI GAWTC 5 cut(s) 295, 308, 752, 792, 1040
PkrI GCNGC 9 cut(s) 155, 369, 535, 839, 842, 882, 885, 1165, 1373
PleI GAGTC 1 cut(s) 418
PpsI GAGTC 1 cut(s) 418
PscI ACATGT 1 cut(s) 939
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 405
PspGI CCWGG 1 cut(s) 405
PspN4I GGNNCC 2 cut(s) 6, 678
PstI CTGCAG 2 cut(s) 885, 1168
PstNI CAGNNNCTG 1 cut(s) 1248
PsuI RGATCY 3 cut(s) 4, 676, 1399
PvuII CAGCTG 1 cut(s) 880
RsaI GTAC 4 cut(s) 248, 317, 1075, 1108
RsaNI GTAC 4 cut(s) 247, 316, 1074, 1107
RseI CAYNNNNRTG 2 cut(s) 41, 1319
SacI GAGCTC 1 cut(s) 69
SacII CCGCGG 1 cut(s) 844
SaqAI TTAA 2 cut(s) 360, 687
SatI GCNGC 9 cut(s) 154, 368, 534, 838, 841, 881, 884, 1164, 1372
Sau3AI GATC 7 cut(s) 4, 634, 640, 676, 1138, 1393, 1399
ScaI AGTACT 1 cut(s) 1108
SchI GAGTC 1 cut(s) 418
ScrFI CCNGG 2 cut(s) 84, 407
SduI GDGCHC 4 cut(s) 69, 451, 585, 1316
SfaNI GCATC 2 cut(s) 392, 985
SfcI CTRYAG 3 cut(s) 436, 881, 1164
Sfr303I CCGCGG 1 cut(s) 844
SfuI TTCGAA 1 cut(s) 159
SgrBI CCGCGG 1 cut(s) 844
SmiMI CAYNNNNRTG 2 cut(s) 41, 1319
SmlI CTYRAG 3 cut(s) 68, 857, 1077
SmoI CTYRAG 3 cut(s) 68, 857, 1077
SpeI ACTAGT 1 cut(s) 1115
Sse9I AATT 2 cut(s) 1012, 1364
SseBI AGGCCT 1 cut(s) 855
SsiI CCGC 4 cut(s) 381, 837, 841, 843
SspI AATATT 1 cut(s) 628
SspMI CTAG 3 cut(s) 123, 1002, 1116
SstI GAGCTC 1 cut(s) 69
StuI AGGCCT 1 cut(s) 855
StyD4I CCNGG 2 cut(s) 82, 405
StyI CCWWGG 2 cut(s) 203, 528
TaaI ACNGT 3 cut(s) 806, 905, 1127
TaiI ACGT 1 cut(s) 822
TaqI TCGA 3 cut(s) 11, 159, 1257
TasI AATT 2 cut(s) 1012, 1364
TatI WGTACW 1 cut(s) 1106
TauI GCSGC 2 cut(s) 840, 843
TfiI GAWTC 5 cut(s) 295, 308, 752, 792, 1040
Tru1I TTAA 2 cut(s) 360, 687
Tru9I TTAA 2 cut(s) 360, 687
TscAI CASTG 2 cut(s) 831, 1253
TseFI GTSAC 1 cut(s) 1121
TseI GCWGC 7 cut(s) 153, 367, 533, 880, 883, 1163, 1371
Tsp45I GTSAC 1 cut(s) 1121
TspGWI ACGGA 2 cut(s) 119, 320
TspRI CASTG 2 cut(s) 831, 1253
XagI CCTNNNNNAGG 1 cut(s) 527
XapI RAATTY 1 cut(s) 1012
XceI RCATGY 1 cut(s) 943
XspI CTAG 3 cut(s) 123, 1002, 1116
ZrmI AGTACT 1 cut(s) 1108
Zsp2I ATGCAT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.