pycom12g00110
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011692
Physical Location & Seq
Forward (+)
190676 .. 192526
1851 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g00110.1

Sequence Viewer

Length: 1437 bp
ATGGATCTATCGATGGAAGCACAAACAGAAACAGGCATGGTGGTGAATAGAGGAATATCAGACGAGCTCAAGGGGTTGCCTCCGGGTTTTCGGTTTCATCCCACGGATGAAGAAATCATAACTAGCTATCTCATAAAGAAGGTCATCAACAGCCGCTTCGAAGCTGCTGCAATTGGCGAAGCTGATTTGAACAAGTGCGAACCTTGGGATTTGCCTAAAAAAGCAAAGATGGGAGAGAAGGAATGGTACTTCTTCTGCCAGAGAGACAGGAAATATCCGACGGGGATGAGGACGAATCGAGCGACGGAATCAGGGTACTGGAAGGCCACAGGAAAAGATAAAGAGATATTCAATAAGATTAAAAAGCAGCAGCAGCAGGGTGGGAAAGGTGCAGGAGGAGGGTGCCTGGTTGGGATGAAGAAGACTCTTGTGTTCTACAGAGGGAGAGCCCCCAAAGGAGAGAAGACCAACTGGGTCATGCATGAATACAGGCTTGATGGCAAAATCCCACCCTACAATAACCTCTCCAAGGCTGCAAGGGATGAATGGGTTGTGTGCAGGGTTTTCCACAAGAACATGGGGCTCAAAAAAACCCTTAGCCCAACTAGGATGAACTCTTTTGGGAATATTGGGGATCATGATCTCCTGGATTGTTCTTCCCTCCCACCTCTTATGGATCCCACTCTTGATACTTCCAACGTCATGACCAACAAACATTCTTCAGGCCATGGAGACAATGACTTCAAGCCCGATCCCATTATCACAGTGGCACCACCATCGGATCATCATGGAATCAGCACTAACAGTTACCTCAACTACTTTTCAGTGGGGGGAGGCGGCGGCGTTCAAGCCCTCAAGCAGAGTTACAACTTTCAGCTGCATCCTACCAGTAACTACAACAGTCACCAAGCCAGTAGTCTCAGTAACCCTTCCAACATGTTACATCCTCCACCTCTTTATCATTATCATCAGCATCACCAGTTTCAAAATCCTTCTAGTTTCTCTAATTTCCAGCCAAATCAGATGATGAGCAATTCTGATTATTTTCAACAAGGGATGATGAGAGGTGCTCAAGGGCAAGGGTCTGTGTTTCGAACAAGTACTAGTACTAGTAGTGACCATGATGACGATGATCAGGCCATTCCAAGAGCAATAGCTAAAGACAACAACAAAAACAAGATGAAGAATAATATTGAAACATCGTCATCTGGCTTAGGAGGAGGCTCAGACAGGCAGTGCAAGGTCGAGCAGTTTTCGACCAACCAGTCTATGTTTAGCCTCTCCCAAGACACCGAATTGAGCACGGATATGAACACTAACGAAATATCATCATCATTTGTTTCAAAGCAAGAATTGGGCAGCACCAACAACTCATCCTATGATCAGGATCCATCTGTTGTTCCCCTTTCCGATATTGAAGACTTGTGGGACTTTTGA

Protein Analysis

479

Amino Acids

53.38

Weight (kDa)

6.6

Isoelectric Point (pI)

34.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 27 - 165 3.2e-48 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011692)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04060 AT5G18270 AT5G18270
fragaria_vesca FvH4_1g27900
malus_domestica MD12G1001200.v1.1 MD14G1001200.v1.1
prunus_persica Prupe.7G001400_v2.0.a1
pyrus_communis pycom12g00110 pycom49g00070
rosa_chinensis RchiOBHm_Chr3g0497471
rosa_laevigata RLG00000022457
rosa_multiflora Rmu_sc0008086.1_g000018
rosa_roxburghii Rroxscaffold_6G00387990
rosa_rugosa Rorug03G0293100
rosa_samantha Rh3AG336800 Rh3BG375100 Rh3CG371100 Rh3DG371800
rosa_wichuraiana Rw3G029600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1264
AccB1I GGYRCC 2 cut(s) 402, 769
AciI CCGC 3 cut(s) 154, 837, 840
AclWI GGATC 8 cut(s) 12, 642, 671, 684, 746, 789, 1382, 1395
AcuI CTGAAG 1 cut(s) 705
AfaI GTAC 4 cut(s) 248, 317, 1102, 1108
AfiI CCNNNNNNNGG 1 cut(s) 529
AflIII ACRYGT 1 cut(s) 936
AgsI TTSAA 9 cut(s) 190, 352, 745, 848, 986, 1049, 1196, 1344, 1418
AhlI ACTAGT 2 cut(s) 1103, 1109
AjnI CCWGG 2 cut(s) 405, 645
AluBI AGCT 6 cut(s) 67, 126, 164, 182, 877, 1157
AluI AGCT 6 cut(s) 67, 126, 164, 182, 877, 1157
Alw21I GWGCWC 3 cut(s) 69, 1072, 1304
Alw26I GTCTC 3 cut(s) 258, 726, 923
AlwI GGATC 8 cut(s) 12, 642, 671, 684, 746, 789, 1382, 1395
AoxI GGCC 3 cut(s) 324, 724, 1137
ApeKI GCWGC 8 cut(s) 164, 167, 367, 370, 373, 533, 877, 1359
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 3 cut(s) 55, 896, 968
AsuII TTCGAA 2 cut(s) 159, 1093
BamHI GGATCC 2 cut(s) 676, 1387
BanI GGYRCC 2 cut(s) 402, 769
BanII GRGCYC 3 cut(s) 69, 451, 585
BarI GAAGNNNNNNTAC 2 cut(s) 230, 262
BbsI GAAGAC 3 cut(s) 428, 470, 1425
Bbv12I GWGCWC 3 cut(s) 69, 1072, 1304
BbvI GCAGC 8 cut(s) 151, 154, 379, 382, 385, 520, 864, 1371
BccI CCATC 5 cut(s) 7, 223, 491, 784, 1399
BcgI CGANNNNNNTGC 4 cut(s) 149, 183, 759, 793
BciT130I CCWGG 2 cut(s) 407, 647
BclI TGATCA 2 cut(s) 1132, 1381
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 3 cut(s) 258, 726, 923
BcuI ACTAGT 2 cut(s) 1103, 1109
BfaI CTAG 5 cut(s) 123, 606, 996, 1104, 1110
BfmI CTRYAG 1 cut(s) 436
BmcAI AGTACT 2 cut(s) 1102, 1108
Bme1390I CCNGG 3 cut(s) 84, 407, 647
BmiI GGNNCC 4 cut(s) 404, 678, 771, 1389
BmrFI CCNGG 3 cut(s) 84, 407, 647
BmrI ACTGGG 1 cut(s) 481
BmsI GCATC 2 cut(s) 889, 982
BmuI ACTGGG 1 cut(s) 481
BpiI GAAGAC 3 cut(s) 428, 470, 1425
BplI GAGNNNNNCTC 2 cut(s) 1054, 1086
Bpu10I CCTNAGC 2 cut(s) 596, 1213
Bpu14I TTCGAA 2 cut(s) 159, 1093
BpuEI CTTGAG 3 cut(s) 53, 839, 1056
BpuMI CCSGG 1 cut(s) 84
Bsa29I ATCGAT 1 cut(s) 11
BsaBI GATNNNNATC 2 cut(s) 639, 1386
BsaJI CCNNGG 4 cut(s) 102, 203, 528, 727
Bsc4I CCNNNNNNNGG 1 cut(s) 529
Bse1I ACTGG 6 cut(s) 323, 476, 888, 912, 979, 1264
Bse8I GATNNNNATC 2 cut(s) 639, 1386
BseBI CCWGG 2 cut(s) 407, 647
BseCI ATCGAT 1 cut(s) 11
BseDI CCNNGG 4 cut(s) 102, 203, 528, 727
BseJI GATNNNNATC 2 cut(s) 639, 1386
BseLI CCNNNNNNNGG 1 cut(s) 529
BseMII CTCAG 2 cut(s) 934, 1239
BseNI ACTGG 6 cut(s) 323, 476, 888, 912, 979, 1264
BseRI GAGGAG 2 cut(s) 411, 1233
BseXI GCAGC 8 cut(s) 151, 154, 379, 382, 385, 520, 864, 1371
BsgI GTGCAG 2 cut(s) 411, 577
BshFI GGCC 3 cut(s) 326, 726, 1139
BshNI GGYRCC 2 cut(s) 402, 769
BshVI ATCGAT 1 cut(s) 11
BsiHKAI GWGCWC 3 cut(s) 69, 1072, 1304
BsiSI CCGG 1 cut(s) 83
BslI CCNNNNNNNGG 1 cut(s) 529
BsmAI GTCTC 3 cut(s) 258, 726, 923
BsnI GGCC 3 cut(s) 326, 726, 1139
Bsp119I TTCGAA 2 cut(s) 159, 1093
Bsp1286I GDGCHC 5 cut(s) 69, 451, 585, 1072, 1304
Bsp143I GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1381, 1387
Bsp19I CCATGG 1 cut(s) 727
BspACI CCGC 3 cut(s) 154, 837, 840
BspANI GGCC 3 cut(s) 326, 726, 1139
BspCNI CTCAG 2 cut(s) 933, 1238
BspDI ATCGAT 1 cut(s) 11
BspHI TCATGA 2 cut(s) 637, 702
BspLI GGNNCC 4 cut(s) 404, 678, 771, 1389
BspPI GGATC 8 cut(s) 12, 642, 671, 684, 746, 789, 1382, 1395
BspT104I TTCGAA 2 cut(s) 159, 1093
BspT107I GGYRCC 2 cut(s) 402, 769
BsrI ACTGG 6 cut(s) 323, 476, 888, 912, 979, 1264
BssECI CCNNGG 4 cut(s) 102, 203, 528, 727
BssMI GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1381, 1387
BssT1I CCWWGG 3 cut(s) 203, 528, 727
Bst2UI CCWGG 2 cut(s) 407, 647
Bst4CI ACNGT 3 cut(s) 766, 806, 902
BstBI TTCGAA 2 cut(s) 159, 1093
BstDEI CTNAG 4 cut(s) 596, 920, 1213, 1225
BstDSI CCRYGG 2 cut(s) 102, 727
BstENI CCTNNNNNAGG 1 cut(s) 527
BstKTI GATC 9 cut(s) 7, 637, 643, 679, 754, 784, 1135, 1384, 1390
BstMAI GTCTC 3 cut(s) 258, 726, 923
BstMBI GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1381, 1387
BstMWI GCNNNNNNNGC 1 cut(s) 373
BstNI CCWGG 2 cut(s) 407, 647
BstNSI RCATGY 1 cut(s) 940
BstSCI CCNGG 3 cut(s) 82, 405, 645
BstSFI CTRYAG 1 cut(s) 436
BstV1I GCAGC 8 cut(s) 151, 154, 379, 382, 385, 520, 864, 1371
BstV2I GAAGAC 3 cut(s) 428, 470, 1425
BstX2I RGATCY 3 cut(s) 4, 676, 1387
BstYI RGATCY 3 cut(s) 4, 676, 1387
Bsu15I ATCGAT 1 cut(s) 11
BsuRI GGCC 3 cut(s) 326, 726, 1139
BsuTUI ATCGAT 1 cut(s) 11
BtgI CCRYGG 2 cut(s) 102, 727
BtsI GCAGTG 1 cut(s) 1241
BtsIMutI CAGTG 3 cut(s) 771, 831, 1241
CciI TCATGA 2 cut(s) 637, 702
ClaI ATCGAT 1 cut(s) 11
Csp6I GTAC 4 cut(s) 247, 316, 1101, 1107
CviQI GTAC 4 cut(s) 247, 316, 1101, 1107
DdeI CTNAG 4 cut(s) 596, 920, 1213, 1225
DpnI GATC 9 cut(s) 6, 636, 642, 678, 753, 783, 1134, 1383, 1389
DpnII GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1381, 1387
DrdI GACNNNNNNGTC 1 cut(s) 1264
DseDI GACNNNNNNGTC 1 cut(s) 1264
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 3 cut(s) 203, 528, 727
Eco24I GRGCYC 3 cut(s) 69, 451, 585
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 705
EcoICRI GAGCTC 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 527
EcoRII CCWGG 2 cut(s) 405, 645
EcoT14I CCWWGG 3 cut(s) 203, 528, 727
EcoT22I ATGCAT 1 cut(s) 483
EcoT38I GRGCYC 3 cut(s) 69, 451, 585
ErhI CCWWGG 3 cut(s) 203, 528, 727
FbaI TGATCA 2 cut(s) 1132, 1381
FriOI GRGCYC 3 cut(s) 69, 451, 585
FspBI CTAG 5 cut(s) 123, 606, 996, 1104, 1110
HaeIII GGCC 3 cut(s) 326, 726, 1139
HapII CCGG 1 cut(s) 83
HinfI GANTC 4 cut(s) 295, 308, 424, 792
HpaII CCGG 1 cut(s) 83
HphI GGTGA 3 cut(s) 55, 896, 968
Hpy188I TCNGA 7 cut(s) 61, 279, 781, 1023, 1039, 1228, 1411
Hpy188III TCNNGA 4 cut(s) 638, 686, 703, 1385
Hpy99I CGWCG 2 cut(s) 283, 307
HpyAV CCTTC 5 cut(s) 133, 232, 316, 939, 1002
HpyCH4III ACNGT 3 cut(s) 766, 806, 902
HpyCH4IV ACGT 1 cut(s) 699
HpyCH4V TGCA 7 cut(s) 170, 392, 481, 536, 558, 880, 1239
HpyF10VI GCNNNNNNNGC 1 cut(s) 373
HpyF3I CTNAG 4 cut(s) 596, 920, 1213, 1225
HpySE526I ACGT 1 cut(s) 699
Ksp22I TGATCA 2 cut(s) 1132, 1381
Kzo9I GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1381, 1387
Lsp1109I GCAGC 8 cut(s) 151, 154, 379, 382, 385, 520, 864, 1371
LweI GCATC 2 cut(s) 889, 982
MaeI CTAG 5 cut(s) 123, 606, 996, 1104, 1110
MaeII ACGT 1 cut(s) 699
MaeIII GTNAC 7 cut(s) 806, 863, 890, 902, 923, 939, 1115
MalI GATC 9 cut(s) 6, 636, 642, 678, 753, 783, 1134, 1383, 1389
MboI GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1381, 1387
MboII GAAGA 9 cut(s) 122, 244, 430, 433, 475, 648, 711, 1195, 1430
MfeI CAATTG 1 cut(s) 171
MflI RGATCY 3 cut(s) 4, 676, 1387
MhlI GDGCHC 5 cut(s) 69, 451, 585, 1072, 1304
MluCI AATT 5 cut(s) 171, 1006, 1033, 1295, 1352
MlyI GAGTC 1 cut(s) 418
MmeI TCCRAC 3 cut(s) 302, 720, 957
Mph1103I ATGCAT 1 cut(s) 483
MseI TTAA 1 cut(s) 360
MslI CAYNNNNRTG 2 cut(s) 41, 1307
MspA1I CMGCKG 1 cut(s) 877
MspI CCGG 1 cut(s) 83
MspR9I CCNGG 3 cut(s) 84, 407, 647
MunI CAATTG 1 cut(s) 171
MvaI CCWGG 2 cut(s) 407, 647
MwoI GCNNNNNNNGC 1 cut(s) 373
NciI CCSGG 1 cut(s) 84
NcoI CCATGG 1 cut(s) 727
NdeII GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1381, 1387
NlaIV GGNNCC 4 cut(s) 404, 678, 771, 1389
NmuCI GTSAC 2 cut(s) 902, 1115
NsiI ATGCAT 1 cut(s) 483
NspI RCATGY 1 cut(s) 940
NspV TTCGAA 2 cut(s) 159, 1093
PagI TCATGA 2 cut(s) 637, 702
PciI ACATGT 1 cut(s) 936
PcsI WCGNNNNNNNCGW 1 cut(s) 299
PfeI GAWTC 3 cut(s) 295, 308, 792
PfoI TCCNGGA 1 cut(s) 645
PleI GAGTC 1 cut(s) 418
PpsI GAGTC 1 cut(s) 418
PscI ACATGT 1 cut(s) 936
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 2 cut(s) 405, 645
PspGI CCWGG 2 cut(s) 405, 645
PspN4I GGNNCC 4 cut(s) 404, 678, 771, 1389
PsuI RGATCY 3 cut(s) 4, 676, 1387
PvuII CAGCTG 1 cut(s) 877
RsaI GTAC 4 cut(s) 248, 317, 1102, 1108
RsaNI GTAC 4 cut(s) 247, 316, 1101, 1107
RseI CAYNNNNRTG 2 cut(s) 41, 1307
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 1 cut(s) 360
Sau3AI GATC 9 cut(s) 4, 634, 640, 676, 751, 781, 1132, 1381, 1387
ScaI AGTACT 2 cut(s) 1102, 1108
SchI GAGTC 1 cut(s) 418
ScrFI CCNGG 3 cut(s) 84, 407, 647
SduI GDGCHC 5 cut(s) 69, 451, 585, 1072, 1304
SfaNI GCATC 2 cut(s) 889, 982
SfcI CTRYAG 1 cut(s) 436
SfuI TTCGAA 2 cut(s) 159, 1093
SmiMI CAYNNNNRTG 2 cut(s) 41, 1307
SmlI CTYRAG 3 cut(s) 68, 854, 1071
SmoI CTYRAG 3 cut(s) 68, 854, 1071
SpeI ACTAGT 2 cut(s) 1103, 1109
Sse9I AATT 5 cut(s) 171, 1006, 1033, 1295, 1352
SsiI CCGC 3 cut(s) 154, 837, 840
SspI AATATT 2 cut(s) 628, 1192
SspMI CTAG 5 cut(s) 123, 606, 996, 1104, 1110
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 3 cut(s) 82, 405, 645
StyI CCWWGG 3 cut(s) 203, 528, 727
TaaI ACNGT 3 cut(s) 766, 806, 902
TaiI ACGT 1 cut(s) 702
TaqI TCGA 6 cut(s) 11, 159, 298, 1093, 1245, 1256
TasI AATT 5 cut(s) 171, 1006, 1033, 1295, 1352
TatI WGTACW 2 cut(s) 1100, 1106
TauI GCSGC 3 cut(s) 156, 840, 843
TfiI GAWTC 3 cut(s) 295, 308, 792
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TscAI CASTG 3 cut(s) 771, 831, 1241
TseFI GTSAC 2 cut(s) 902, 1115
TseI GCWGC 8 cut(s) 164, 167, 367, 370, 373, 533, 877, 1359
Tsp45I GTSAC 2 cut(s) 902, 1115
TspDTI ATGAA 8 cut(s) 86, 123, 431, 498, 558, 626, 1196, 1325
TspGWI ACGGA 3 cut(s) 119, 320, 1319
TspRI CASTG 3 cut(s) 771, 831, 1241
XagI CCTNNNNNAGG 1 cut(s) 527
XceI RCATGY 1 cut(s) 940
XcmI CCANNNNNNNNNTGG 1 cut(s) 763
XspI CTAG 5 cut(s) 123, 606, 996, 1104, 1110
ZrmI AGTACT 2 cut(s) 1102, 1108
Zsp2I ATGCAT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.