MD12G1002400.v1.1

HEAT repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002980
Physical Location & Seq
Forward (+)
399666 .. 419085
19420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1002400.v1.1.491

Sequence Viewer

Length: 6849 bp
ATGGCGAAGAAGTATGTGACAACAGAGAATGCGCCCCTCTCTCAGTTCGGTGTACTGGTAGCGCAATTGGAGTCCATAGTCGCCTCAGCATCCCATAAGCCTCCGGAGCCACTTCTATGCTTTGATCTCCTCTCCGATCTCATCTCCGCCATTAACGAAGAGCCCAAGGAATCCATTTTGTTGTGGCAAAGGAGATGCGAGGACGCATTATATTCCTTGCTTATTCTTGGTGCTCGGCGACCTGTCCGCCATCTGGCTTCAGTTGCTATGGCGAGGGTTATATCTAAGGGAGATGGCATTTCTATATATTCTAGAGTGAGCAGTCTCCAAGGCTTTCTCTCTGATGGGAGGAGGAGTGAACCGCAAAAACTTGCTGGTGCTGCACAATGCTTAGGAGAATTATATAGACATTTTGGGAGGCGAATCACATCGGGTTTACTTGAAACGACTATCATTGCCATGAAACTTTTCAAGTTTAATGAGGAATTTGTGAGACAAGAAGCTTTATATATGCTTCAAAATGCTTTGGAAGGCTCTGGTGGAAATGCAACAGCTTCAGCATATACAGAGGCATTTCGTCTCATCATGCGGTTTGCTGTTGGTGATAAATCATTTCTTGTTAGAATTGCTGCTGCCAGATGTCTGAAGGCATTTGCCATTATCGGAGGACCGGGTCTAGGGGTGGGAGAACTAGACAGTTCAGCTTCTTATTGTGTCAAGGCCCTTGAAGATCCTGTTTCATCTGTCCGTGATGCATTTGCTGAAGCATTGGGTTCGTTGCTTGCTCTTGGAATGAATCCTCATGCACTGGTTCAACCAAGGGGGAAAGGACCTTTTCCTCCAAGAAAGAAACTGGAAGGTGGTTTGCATAGGCATTTAGCTTTGCCTTTCACAAAAGTTGGAGCTCGGTCAAAGGACGTGCGAGTTGGCATAACCTTGTCTTGGGTATTCTTTTTACAGGCTATTCGTCTAAAATACATGCATCCAGATAGTGAGCTACAAAACTATGCAATTCAGGTTATGGAAATGCTTTGTTCCGATAATTCTGTTGATGCCTATGCACTGGCTTGTGTTCTTTATATCCTTCGTGTTGGTGTTACTGATCAAATGACAGAGCCTACGCAAAGGAGTTTTTTGGGTTTTCTTGGGAATCAGCTTATGTCTCTAGATGCCAGTCCTTCCATGAAAATTGCTGCCCTACGTACTGCATCATATACCTTGAAAACGCTGGGAGAGGTTCCTGTTGAATTCAAGGAAGTTCTTGACAACACTGTTGTTGCAGCAGTATCTCATTCTTCACAACTCGTTCGAATTGAGGCTGCTTTGACATTACGTGCACTGGCTGAAGTTGATCCCACCTGTGTTGGTGGGTTAATTTCTTATGGAGTGACCATGCTGAATGCTTTGAGAGAAAATTTGGCATTTGAGAAGGGGAGCACTTTGCAATTAGAGCTTGATTCTTTGCATGGACAAGCTACTGTCTTGGCAGCTCTGGTGTCCATTTCCCCAAAATTACCTCTCGGTTTTCCTGCCAGATTGCCCAGGTCAATCCTTGAAGTTTCAAAGAAGATGCTGACTGAATCTAGTCGGAACCCTCAGGCAGCTACAATTGAAAAAGAAGCTGGGTGGTTACTTTTGTCATCCCTGTTAGCTTCTATGCCAAAGGAGGAACTTGAAGACCAGGTTTTTGATATTCTTTCCTTGTGGGCTTCCCTCTTTACTGGAAATCCAGAAGATGAAACTAACCATACTGGAGATTTGATAAGTAGGATTCGCATGTGGTCTGCGGCAGTTGATGCACTTACTGCATTTTTAAGATGCTTTCTTTCTCCTAATGATGGGAATAATGGGATTTTGCTTCAACCTGTACTTGTATACCTGAGTAGGGCTTTATCGTATATATCACTGATAGCGGCCAAGCAACTGCCAAATGTGAAGCCTGCTTTGGATATATTTATCGTCAGGACATTAATAGCTTACCAGTCTCTTCCTGATCCAACAGCATATAAGAATGACCACCGCATGGTTCTTCAAATTTGCACAAGTCCATTCATAGAGGCTTCTGGATGTGAAGAAAGTACATGCTTGAGGTTTCTGTTAGACAAAAGAGATGCATGGTTGGGTCCCTGGATTCCTGGAAGAGATTGGTTTGAAGATGAACTTCGTGCTTTTCAAGGTGGAAAAGATGGCCTAATTCCTTGTGTCTGGGAGAATGAAATTTGTAGCTTTCCTCAGCCGGAACCAGTAAACAAGACATTGGTGAACCAGATGCTCCTCTGCTTTGGCGTCTTGTTTGCCTCGCAGGATAGTGGTGGGATGCTGTCACTTCTTGGAACGATTGAGCAGTGCCTGAAAGCTGGAAAAAAGCAACCATGGCATGCAGGGAGCATAACTAATATATGTGTGGGGTTACTGTCAGGATTTAAGGCTTTGCTTTCCTTGCGTCCCCAACCATTATCACTTGAGATACTAAACTCAGCACAGGCTATATTTCAGAGCATTCTGGCAGAGGGAGATATCTGTCCATCACAGCGTAGGGCTTCATCAGAATGTCTGGGTCTTTTAGCTCGTCTTGGAAATGATATCTTTACAGCAAGAATGGTAACTAGATCTCTGCTTGGTGACTTAACTGGGGCAACAGATTCAAACTATGCTGGATCAATTGCTTTTGCTCTTGGCTGCATTCATCGCAGTGCTGGAGGCATGGCATTGTCAACTTTAGTGCCTTCAACTGTGAGCTCAATCTCGCTGCTTTCTAAAAGTTCAATAGCTGGTTTACAGATCTGGTCTTTGCATGGGCTTCTGTTAACCATTGAAGCTGCTGGCTTGTCCTATGTATCTCAAGTCCAGGCAACACTAGGTCTTTCTCTGGACATTCTTTTGTCCGAGGAGAATGGATGGGTTGCACTTCAGCAAGGTGTTGGCCGTCTTATAAATGCTATTGTTGCTGTTCTTGGTCCTGAACTTGCCCCTGGCAGTATTTTCTTTTCACGTTGCAAGTCTGTTGTTTCCGAGATAAGCTCTGGGCAAGAAACAGCAACGATGCTTGAGAGTGTACGCTTTACCCAGCAACTTGTTCTTTTTGCACCACAAGCTGTTTCCGTGCACACCCATGTGCAAACTCTTCTACCTACTTTGTCCTCAAGACAGCCAGTGTTAAGGCATCTAGCTGTTTCCACTCTACGACATCTGATTGAAAAAGATCCGGTTTCCATTGTTGTCGAGCAAATAGAAGAGAAGTTATTTCACATGCTGGATGAAGAAACAGATTCCGAGATAGGGGATCTGGTGCGCACCACAATCATGCGATTGCTCTATGCATCATGCCCTTCCTGTCCATCCCATTGGATATCAATTTGTCGTAATGTGATTCTTGCCACATCAATGCGAAGAAATGCTGATAGTAGTAGCAGCTTAGAAAATTATCCCTCAAAGGGTACAGAGGGTGACCCAAGTGTAAATTTTGGAGAAGACGATGAGAACATGGTTTCTAGCACCTTAGGCCCCCATGGTATTCTGAATAGAGATAAACACCTCAGATACCGAACCAGAGTTTTTGCTGCTGAGTGTTTGAGTTACCTACCTAGAGCTGTTGGAAAGAATCCAGCTCATTTTGATCTTTGTACTGCTAGGAGCCAACCTACTAATGGACAGGCCTCTAGTGATTGGCTCGTCCTCCATATACAAGAACTTATAGCCCTTGCATACCAGATAAGCACGATCCAGTTGGAAAACTTGCAGCCAATTGGTGTGGGACTTCTGAGTACCATTACAGATAAGTTTGAAAGGATACCTGACCCAGAGCTTCCAGGGCACCTCCTACTGGAACAGTATCAGGCCCAGTTAGTATCTGCTGTCCGCACTGCCTTAGACTCGTCCTCTGGCCCTATTCTATTGGAAGCAGGCTTCCTGCTTGCTACAAAGATACTGACTAGTGGAATTATTAAGGGCGATCAAATTGCTGTTAAACGCATATATTCATTGATTTCACGACCATTGACTGAATTTAAGGACCTGTATTATCCTTCATTTGCAGAATGGGTCTCATGCAAGATCAAGATAAGACTTCTTGCTGCTCATGCCTCTCTCAAATGTTATACATATGCGTTCTTGAGGAGACACCATAGTGGGGTTCCAGATGAATATCTAGCATTGTTACCATTATTTTCAAAGAGTTCAAGTATGCTTGGGAAGTACTGGATTAATGTTTTGAAGGATTACAGTTATGTATTCTTGTGCTTGCATCTCAAGACAAAGTGGAATCCATTTCTTGATGGAATTCAGTCACCTCTGGTATCATCAAAGTTGCAACCTTGCTTAGAAGAATCCTGGCCAGTAATATTGCAAGCAATTGCATTGGATGCAGTTCCTGTGAATATTGAAGAGGAGGAGTATTCAAACTCCACTACTCAAAATAAATCAAGAAATAGTTTATTGTCCGGACATCACATGGTTGAACTGGAATCCGAAGAATATCAGTTTCTTTGGGGATTTGCTCTACTTGTCTTATTTCAGGGCCAATATTCTACCCTTGGTGAACTGAAAAGTCCAATTTATTTCATTAAGACTAGTAACGGTGGAGATTCAGCTAGTGAAGAATTATCTTTCCCAGGAATTAAGTTATATGAAATTGCATTACCGGTTTTCCAGTTTCTGGCGACCAAAAGGTTTGCCAGTGCGGGATTTCTCACTGTGGACATATGCAGAGAACTGCTACAGGTTTTCTCATATTCTATGTGCATGGATAATTCTTGGGATAGTCTTTCAGTGTCTGTTATATCAGAGATTGTGAAGAACTGCCCTGAATCTTTTTATGAAGTGGACAACTTTGCGTATTTGGCAATGGAACTTTGTTTGGCTTATCTTTACAAAGTATTTCAAAGTAAGGCAATATCTTTGGACAAACCCTCGGAGGATTTGATATCCACATTATTTATCACTGCTAAAACACTTGTAAACTGTTTTCAACCAAAGACACAACTAATCTCTGCTGCATTGGCGTTTCTTTTAATTGGTTACAAGGGAATCAGAGAAGCCTCAACTGAGTTTTGTTTCTCCAAGGTGGACGATTTTTTCAAGTGCACAGGTCTATTAGTGAAAAGATTTATTGATGATAAATCCGGTGTTGGTGAAGATGGTCTTCTTCATATGCAAAAATTATTGGGAACTTGTCTGAATGTGATAACTGATTTGACCGAGGATTGCATTAAGTCCATTCATCTGCAGGAAAATAAGAGATCTGACTTGCACATACTACTGCAAACAAAGCTTGCTTTCTCTCTTGAACAAACTATATCATTTGCCAAGTTGGGTTACGAAATAAATTATCTTGAAGAGAATAGAGATGGTGATTCCGTATATTCTACTATGTTTAAATACTGCACAAAATGTGTCCAAACTGTACTTACCGATTCAAATATACAGGTTCAATCAATTGGCTTACAAGTGCTAAAAAGTTTGGTACAGAAAACCCCAAATGTGGAAGGCAACAATTTTTTAATGCTCTTTGTTGGGGAGCTTACTGCAGATTTTTTTGTGATAATCCAGAATGCTTTAAAGAAACCTGTAACTGAAAAATCAGCAACTGTTGCTGGTGAATGCTTAAGACTCTTAGTAGTGCTGCAGACACTTTCAAAACCTAGTGAATGTCAGAGAGGTTTTATGAATTTACTCTTGGAAGCCGTAATCGTAGTTTTCAAGGCTTCAGAAGAAGGTTCTTCACTGGAAGTCGATACATTAAGAAGCACTGCTGTAAGGCTTGTTTCTCATCTTGCTCAGCTTCCTTCGTCAGCAGTTCACTTCAAGGATATATTGCTGTCTATGCCTGTAACACACCGACAGCAACTTCAGGGGTTCATCCGTGCGTCTGTGACACAAGAGCACAATGCAACCCAGCTGAAGCCCACAACTCCGTCATTAGAGATAAAATTACCAGTTCCAACAGAGGCAAGCAAAGAGAAACCTCCACCACCAGCCACCACAGCGCATTCTCTTTCTGATGACCAAGAAATCGAAGAGGAAGAAGAGGATGAAGATGTTTGGGATGCTTTCCAATCTTTTCCTGCCGCTTTAAAAGCAGCTGAAAGTGAATCAAAAGGAGAAAGCACAGCGGAAGAACCTGACTTGGAAGTGAATACACAGAGTGATTACACTCATGGAGATTCTATTTCGCAACCTCTCAACAACGTAGATGCAGTCGACGATACAGACCATCAAGAGGCTGGTGAAAGGGAGGTGATATCAGATGCCCCTGATGGTCTAAAATCCCCACAGGGTAACGCACCAACCAACAGTACTGAGGCAGAAGAACCCCATGATCTCCAAACTAAGAGCGGTGTTAGTGGACCATGCGATGATCAGCAATTGGAGAGCGACGAGGAAGTGGTTTCCAGACAGGTGGGAATGACATCAGGGTCGAACCAAGTTACTGTGTGCATGCCATCCGAACTTCATCCTACTGATACAGAGGCTGAAGAAACCCGTGATCTCCAAACTAATAGCGGCTTTGTCCCAGCATGTGATGATCAGCATTTGAAGAGAAACAAGGAAGTCGTTTCCAGACAAGAGGGAATGGCAGCAGGGTTGAACCAAGTGAATGCTGAGCGTATGCCATCTGAACATCCTACTGGAGATGCTGAACATATGCCAGTTGAAGTGAATTTAGACCATCATTTGCAGGGGAAAAAAGAGAACCCTGGCAATAAACCAGGCCAGGTATCATCGGATCCTCTACCACTTGATGAGGAAGTATCTGATAAAAGAGAAGACAAGGAAGAGGCGGCTATCGAAAAGTCTTGCCTCGAGCTGCCTGGAGAGAGCAGTAAGGTTAAAGATTCATCGGAAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

2283

Amino Acids

250.5

Weight (kDa)

5.23

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Laa1_Sip1_HTR5 PF20210 1223 - 1408 5.1e-43 Laa1/Sip1/HEATR5 HEAT repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2933
AasI GACNNNNNNGTC 1 cut(s) 6412
Acc16I TGCGCA 1 cut(s) 3293
AccB1I GGYRCC 1 cut(s) 3813
AccB7I CCANNNNNTGG 2 cut(s) 2023, 4654
AccBSI CCGCTC 1 cut(s) 6333
AccI GTMKAC 2 cut(s) 1875, 6198
AccIII TCCGGA 2 cut(s) 103, 4439
AclWI GGATC 9 cut(s) 725, 1346, 1988, 2665, 3197, 3291, 3715, 6719, 6732
AcoI YGGCCR 3 cut(s) 1914, 2923, 4331
AcyI GRCGYC 1 cut(s) 2286
AdeI CACNNNGTG 1 cut(s) 6143
AflII CTTAAG 1 cut(s) 5602
AgeI ACCGGT 1 cut(s) 4639
AhlI ACTAGT 2 cut(s) 3932, 4568
AjiI CACGTC 1 cut(s) 919
AjuI GAANNNNNNNTTGG 4 cut(s) 1928, 1960, 5657, 5689
AleI CACNNNNGTG 2 cut(s) 3113, 4125
Alw21I GWGCWC 8 cut(s) 235, 907, 1339, 1439, 2741, 3108, 5084, 5883
Alw26I GTCTC 7 cut(s) 329, 487, 584, 1167, 1989, 4048, 4111
Alw44I GTGCAC 3 cut(s) 1335, 3104, 5080
AlwI GGATC 9 cut(s) 725, 1346, 1988, 2665, 3197, 3291, 3715, 6719, 6732
AlwNI CAGNNNCTG 6 cut(s) 2350, 4370, 4654, 4772, 5585, 6470
Ama87I CYCGRG 1 cut(s) 6800
Aor13HI TCCGGA 2 cut(s) 103, 4439
ApaLI GTGCAC 3 cut(s) 1335, 3104, 5080
ArsI GACNNNNNNTTYG 2 cut(s) 1670, 1702
AseI ATTAAT 2 cut(s) 1970, 4203
AsiGI ACCGGT 1 cut(s) 4639
Asp700I GAANNNNTTC 1 cut(s) 467
AspLEI GCGC 4 cut(s) 34, 64, 3294, 5986
AsuC2I CCSGG 1 cut(s) 672
AsuII TTCGAA 1 cut(s) 1309
AvaI CYCGRG 1 cut(s) 6800
AvaII GGWCC 6 cut(s) 668, 830, 2123, 2957, 4012, 6344
AxyI CCTNAGG 2 cut(s) 1596, 3499
BaeGI GKGCMC 4 cut(s) 1339, 3108, 3816, 5084
BalI TGGCCA 1 cut(s) 4333
BamHI GGATCC 1 cut(s) 6724
BanI GGYRCC 1 cut(s) 3813
BanII GRGCYC 3 cut(s) 165, 907, 2741
BbsI GAAGAC 4 cut(s) 1683, 3477, 5132, 6771
Bbv12I GWGCWC 8 cut(s) 235, 907, 1339, 1439, 2741, 3108, 5084, 5883
BbvCI CCTCAGC 2 cut(s) 85, 2232
BceAI ACGGC 2 cut(s) 2910, 5666
BcgI CGANNNNNNTGC 4 cut(s) 756, 790, 3941, 3975
BciVI GTATCC 1 cut(s) 3783
BclI TGATCA 3 cut(s) 1102, 6355, 6523
BcnI CCSGG 1 cut(s) 672
BcoDI GTCTC 7 cut(s) 329, 487, 584, 1167, 1989, 4048, 4111
BcuI ACTAGT 2 cut(s) 3932, 4568
BfmI CTRYAG 4 cut(s) 4715, 5222, 5523, 5621
BfrI CTTAAG 1 cut(s) 5602
BfuI GTATCC 1 cut(s) 3783
BglII AGATCT 3 cut(s) 2609, 2781, 5237
BlpI GCTNAGC 2 cut(s) 5775, 6600
BmcAI AGTACT 2 cut(s) 4196, 6295
Bme18I GGWCC 6 cut(s) 668, 830, 2123, 2957, 4012, 6344
BmeT110I CYCGRG 1 cut(s) 6800
BmgBI CACGTC 1 cut(s) 919
BmrI ACTGGG 2 cut(s) 2640, 3835
BmuI ACTGGG 2 cut(s) 2640, 3835
BpiI GAAGAC 4 cut(s) 1683, 3477, 5132, 6771
BplI GAGNNNNNCTC 2 cut(s) 3005, 3037
BpmI CTGGAG 4 cut(s) 1773, 2718, 6648, 6831
Bpu10I CCTNAGC 3 cut(s) 85, 391, 2232
Bpu1102I GCTNAGC 2 cut(s) 5775, 6600
Bpu14I TTCGAA 1 cut(s) 1309
BpuEI CTTGAG 7 cut(s) 2107, 2483, 2826, 3068, 3127, 4132, 4232
BpuMI CCSGG 1 cut(s) 672
BsaAI YACGTR 2 cut(s) 1202, 1334
BsaBI GATNNNNATC 1 cut(s) 4143
BsaHI GRCGYC 1 cut(s) 2286
BsaI GGTCTC 1 cut(s) 4048
BsaWI WCCGGW 5 cut(s) 103, 3205, 4439, 4639, 5120
BsaXI ACNNNNNCTCC 2 cut(s) 340, 370
Bse118I RCCGGY 1 cut(s) 4639
Bse21I CCTNAGG 2 cut(s) 1596, 3499
Bse3DI GCAATG 2 cut(s) 453, 4848
Bse8I GATNNNNATC 1 cut(s) 4143
BseAI TCCGGA 2 cut(s) 103, 4439
BseJI GATNNNNATC 1 cut(s) 4143
BseMI GCAATG 2 cut(s) 453, 4848
BseRI GAGGAG 8 cut(s) 119, 364, 367, 2264, 2903, 4129, 4400, 4403
BseSI GKGCMC 4 cut(s) 1339, 3108, 3816, 5084
BseYI CCCAGC 5 cut(s) 1228, 1622, 3066, 5892, 6511
BsgI GTGCAG 2 cut(s) 366, 5365
BshNI GGYRCC 1 cut(s) 3813
BshTI ACCGGT 1 cut(s) 4639
BsiHKAI GWGCWC 8 cut(s) 235, 907, 1339, 1439, 2741, 3108, 5084, 5883
BsiHKCI CYCGRG 1 cut(s) 6800
BsiSI CCGG 7 cut(s) 104, 671, 2237, 3206, 4440, 4640, 5121
BslFI GGGAC 4 cut(s) 2109, 2430, 3768, 6494
BsmAI GTCTC 7 cut(s) 329, 487, 584, 1167, 1989, 4048, 4111
BsmBI CGTCTC 1 cut(s) 584
BsmFI GGGAC 4 cut(s) 2109, 2430, 3768, 6494
BsmI GAATGC 8 cut(s) 34, 1405, 2499, 2682, 5554, 5603, 5986, 6601
Bso31I GGTCTC 1 cut(s) 4048
BsoBI CYCGRG 1 cut(s) 6800
Bsp119I TTCGAA 1 cut(s) 1309
Bsp13I TCCGGA 2 cut(s) 103, 4439
Bsp1720I GCTNAGC 2 cut(s) 5775, 6600
Bsp19I CCATGG 2 cut(s) 2372, 3508
BspEI TCCGGA 2 cut(s) 103, 4439
BspMAI CTGCAG 3 cut(s) 5226, 5527, 5625
BspPI GGATC 9 cut(s) 725, 1346, 1988, 2665, 3197, 3291, 3715, 6719, 6732
BspQI GCTCTTC 1 cut(s) 153
BspT104I TTCGAA 1 cut(s) 1309
BspT107I GGYRCC 1 cut(s) 3813
BspTI CTTAAG 1 cut(s) 5602
BspTNI GGTCTC 1 cut(s) 4048
BsrBI CCGCTC 1 cut(s) 6333
BsrDI GCAATG 2 cut(s) 453, 4848
BsrFI RCCGGY 1 cut(s) 4639
BssAI RCCGGY 1 cut(s) 4639
BssNAI GTATAC 1 cut(s) 1876
BssNI GRCGYC 1 cut(s) 2286
BssT1I CCWWGG 7 cut(s) 165, 328, 818, 2372, 3508, 4531, 5058
Bst1107I GTATAC 1 cut(s) 1876
BstACI GRCGYC 1 cut(s) 2286
BstAFI CTTAAG 1 cut(s) 5602
BstAPI GCANNNNNTGC 4 cut(s) 1796, 1805, 4361, 5588
BstBAI YACGTR 2 cut(s) 1202, 1334
BstBI TTCGAA 1 cut(s) 1309
BstDSI CCRYGG 2 cut(s) 2372, 3508
BstEII GGTNACC 1 cut(s) 3446
BstHHI GCGC 4 cut(s) 34, 64, 3294, 5986
BstMAI GTCTC 7 cut(s) 329, 487, 584, 1167, 1989, 4048, 4111
BstNSI RCATGY 7 cut(s) 982, 1780, 2085, 2381, 3253, 6439, 6519
BstPI GGTNACC 1 cut(s) 3446
BstSFI CTRYAG 4 cut(s) 4715, 5222, 5523, 5621
BstSLI GKGCMC 4 cut(s) 1339, 3108, 3816, 5084
BstSNI TACGTA 1 cut(s) 1202
BstV2I GAAGAC 4 cut(s) 1683, 3477, 5132, 6771
BstX2I RGATCY 7 cut(s) 730, 2609, 2781, 3202, 3283, 5237, 6724
BstXI CCANNNNNNTGG 2 cut(s) 3345, 6397
BstYI RGATCY 7 cut(s) 730, 2609, 2781, 3202, 3283, 5237, 6724
BstZ17I GTATAC 1 cut(s) 1876
Bsu36I CCTNAGG 2 cut(s) 1596, 3499
BsuI GTATCC 1 cut(s) 3783
BtgI CCRYGG 2 cut(s) 2372, 3508
BtgZI GCGATG 2 cut(s) 2672, 6366
BtrI CACGTC 1 cut(s) 919
BtsI GCAGTG 5 cut(s) 2351, 2698, 3861, 4938, 5745
CaiI CAGNNNCTG 6 cut(s) 2350, 4370, 4654, 4772, 5585, 6470
CfoI GCGC 4 cut(s) 34, 64, 3294, 5986
Cfr10I RCCGGY 1 cut(s) 4639
CseI GACGC 4 cut(s) 212, 2275, 2432, 5853
CsiI ACCWGGT 1 cut(s) 1680
CspAI ACCGGT 1 cut(s) 4639
CspCI CAANNNNNGTGG 4 cut(s) 1345, 1380, 3732, 3767
DraI TTTAAA 3 cut(s) 5374, 5556, 6072
DraIII CACNNNGTG 1 cut(s) 6143
DrdI GACNNNNNNGTC 1 cut(s) 6412
DseDI GACNNNNNNGTC 1 cut(s) 6412
EaeI YGGCCR 3 cut(s) 1914, 2923, 4331
EciI GGCGGA 2 cut(s) 136, 236
Ecl136II GAGCTC 2 cut(s) 905, 2739
Eco105I TACGTA 1 cut(s) 1202
Eco130I CCWWGG 7 cut(s) 165, 328, 818, 2372, 3508, 4531, 5058
Eco147I AGGCCT 1 cut(s) 3656
Eco24I GRGCYC 3 cut(s) 165, 907, 2741
Eco31I GGTCTC 1 cut(s) 4048
Eco32I GATATC 5 cut(s) 2518, 2584, 3351, 4923, 6240
Eco47I GGWCC 6 cut(s) 668, 830, 2123, 2957, 4012, 6344
Eco53kI GAGCTC 2 cut(s) 905, 2739
Eco81I CCTNAGG 2 cut(s) 1596, 3499
Eco88I CYCGRG 1 cut(s) 6800
Eco91I GGTNACC 1 cut(s) 3446
EcoICRI GAGCTC 2 cut(s) 905, 2739
EcoO109I RGGNCCY 5 cut(s) 721, 830, 2123, 3503, 4012
EcoO65I GGTNACC 1 cut(s) 3446
EcoRI GAATTC 2 cut(s) 1247, 4278
EcoRV GATATC 5 cut(s) 2518, 2584, 3351, 4923, 6240
EcoT14I CCWWGG 7 cut(s) 165, 328, 818, 2372, 3508, 4531, 5058
EcoT22I ATGCAT 4 cut(s) 757, 984, 2116, 3322
EcoT38I GRGCYC 3 cut(s) 165, 907, 2741
ErhI CCWWGG 7 cut(s) 165, 328, 818, 2372, 3508, 4531, 5058
Esp3I CGTCTC 1 cut(s) 584
FalI AAGNNNNNCTT 4 cut(s) 2145, 2177, 5124, 5156
FaqI GGGAC 4 cut(s) 2109, 2430, 3768, 6494
FauI CCCGC 1 cut(s) 4672
FauNDI CATATG 4 cut(s) 4102, 4700, 5148, 6642
FbaI TGATCA 3 cut(s) 1102, 6355, 6523
FblI GTMKAC 2 cut(s) 1875, 6198
FriOI GRGCYC 3 cut(s) 165, 907, 2741
FspAI RTGCGCAY 1 cut(s) 3293
FspI TGCGCA 1 cut(s) 3293
GlaI GCGC 4 cut(s) 33, 63, 3293, 5985
GsaI CCCAGC 5 cut(s) 1232, 1626, 3070, 5896, 6515
GsuI CTGGAG 4 cut(s) 1773, 2718, 6648, 6831
HapII CCGG 7 cut(s) 104, 671, 2237, 3206, 4440, 4640, 5121
HgaI GACGC 4 cut(s) 212, 2275, 2432, 5853
HhaI GCGC 4 cut(s) 34, 64, 3294, 5986
Hin1I GRCGYC 1 cut(s) 2286
Hin6I GCGC 4 cut(s) 32, 62, 3292, 5984
HinP1I GCGC 4 cut(s) 32, 62, 3292, 5984
HincII GTYRAC 3 cut(s) 2715, 2808, 6199
HindII GTYRAC 3 cut(s) 2715, 2808, 6199
HindIII AAGCTT 2 cut(s) 501, 5267
HpaI GTTAAC 1 cut(s) 2808
HpaII CCGG 7 cut(s) 104, 671, 2237, 3206, 4440, 4640, 5121
Hpy99I CGWCG 2 cut(s) 6203, 6377
HpyCH4IV ACGT 5 cut(s) 918, 1201, 1333, 2992, 6186
HpySE526I ACGT 5 cut(s) 918, 1201, 1333, 2992, 6186
Hsp92I GRCGYC 1 cut(s) 2286
HspAI GCGC 4 cut(s) 32, 62, 3292, 5984
KflI GGGWCCC 1 cut(s) 2123
Kpn2I TCCGGA 2 cut(s) 103, 4439
Ksp22I TGATCA 3 cut(s) 1102, 6355, 6523
KspAI GTTAAC 1 cut(s) 2808
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 7 cut(s) 106, 902, 1434, 2277, 2385, 3633, 5515
MabI ACCWGGT 1 cut(s) 1680
MaeII ACGT 5 cut(s) 918, 1201, 1333, 2992, 6186
MbiI CCGCTC 1 cut(s) 6333
MfeI CAATTG 7 cut(s) 65, 1608, 2661, 3744, 4350, 5433, 6362
MflI RGATCY 7 cut(s) 730, 2609, 2781, 3202, 3283, 5237, 6724
MlsI TGGCCA 1 cut(s) 4333
MluNI TGGCCA 1 cut(s) 4333
MlyI GAGTC 3 cut(s) 80, 3866, 5601
MmeI TCCRAC 6 cut(s) 880, 1568, 2021, 3574, 3708, 5963
Mox20I TGGCCA 1 cut(s) 4333
Mph1103I ATGCAT 4 cut(s) 757, 984, 2116, 3322
MroI TCCGGA 2 cut(s) 103, 4439
MroXI GAANNNNTTC 1 cut(s) 467
MscI TGGCCA 1 cut(s) 4333
MslI CAYNNNNRTG 9 cut(s) 115, 458, 2548, 2691, 3111, 3113, 3302, 3383, 4125
Msp20I TGGCCA 1 cut(s) 4333
MspA1I CMGCKG 3 cut(s) 5896, 6080, 6110
MspCI CTTAAG 1 cut(s) 5602
MspI CCGG 7 cut(s) 104, 671, 2237, 3206, 4440, 4640, 5121
MunI CAATTG 7 cut(s) 65, 1608, 2661, 3744, 4350, 5433, 6362
Mva1269I GAATGC 8 cut(s) 34, 1405, 2499, 2682, 5554, 5603, 5986, 6601
NciI CCSGG 1 cut(s) 672
NcoI CCATGG 2 cut(s) 2372, 3508
NdeI CATATG 4 cut(s) 4102, 4700, 5148, 6642
NmeAIII GCCGAG 1 cut(s) 214
NmuCI GTSAC 7 cut(s) 16, 1387, 2322, 2621, 3446, 4284, 5869
NsbI TGCGCA 1 cut(s) 3293
NsiI ATGCAT 4 cut(s) 757, 984, 2116, 3322
NspI RCATGY 7 cut(s) 982, 1780, 2085, 2381, 3253, 6439, 6519
NspV TTCGAA 1 cut(s) 1309
OliI CACNNNNGTG 2 cut(s) 3113, 4125
PaeI GCATGC 2 cut(s) 2381, 6439
PaeR7I CTCGAG 1 cut(s) 6800
PceI AGGCCT 1 cut(s) 3656
PciSI GCTCTTC 1 cut(s) 153
PctI GAATGC 8 cut(s) 34, 1405, 2499, 2682, 5554, 5603, 5986, 6601
PdmI GAANNNNTTC 1 cut(s) 467
PflFI GACNNNGTC 1 cut(s) 672
PflMI CCANNNNNTGG 2 cut(s) 2023, 4654
PfoI TCCNGGA 1 cut(s) 2134
PinAI ACCGGT 1 cut(s) 4639
PleI GAGTC 3 cut(s) 79, 3866, 5601
PpsI GAGTC 3 cut(s) 79, 3866, 5601
Ppu21I YACGTR 2 cut(s) 1202, 1334
PpuMI RGGWCCY 3 cut(s) 830, 2123, 4012
PshBI ATTAAT 2 cut(s) 1970, 4203
PsiI TTATAA 1 cut(s) 2933
Psp124BI GAGCTC 2 cut(s) 907, 2741
Psp5II RGGWCCY 3 cut(s) 830, 2123, 4012
PspEI GGTNACC 1 cut(s) 3446
PspFI CCCAGC 5 cut(s) 1228, 1622, 3066, 5892, 6511
PspPPI RGGWCCY 3 cut(s) 830, 2123, 4012
PspXI VCTCGAGB 1 cut(s) 6800
PstI CTGCAG 3 cut(s) 5226, 5527, 5625
PstNI CAGNNNCTG 6 cut(s) 2350, 4370, 4654, 4772, 5585, 6470
PsuI RGATCY 7 cut(s) 730, 2609, 2781, 3202, 3283, 5237, 6724
PsyI GACNNNGTC 1 cut(s) 672
PvuII CAGCTG 2 cut(s) 5896, 6080
RseI CAYNNNNRTG 9 cut(s) 115, 458, 2548, 2691, 3111, 3113, 3302, 3383, 4125
SacI GAGCTC 2 cut(s) 907, 2741
SalI GTCGAC 1 cut(s) 6197
SapI GCTCTTC 1 cut(s) 153
ScaI AGTACT 2 cut(s) 4196, 6295
SchI GAGTC 3 cut(s) 80, 3866, 5601
SexAI ACCWGGT 1 cut(s) 1680
SfcI CTRYAG 4 cut(s) 4715, 5222, 5523, 5621
Sfr274I CTCGAG 1 cut(s) 6800
SfuI TTCGAA 1 cut(s) 1309
SinI GGWCC 6 cut(s) 668, 830, 2123, 2957, 4012, 6344
SlaI CTCGAG 1 cut(s) 6800
SmiMI CAYNNNNRTG 9 cut(s) 115, 458, 2548, 2691, 3111, 3113, 3302, 3383, 4125
SmlI CTYRAG 9 cut(s) 2086, 2462, 2841, 3047, 3142, 4111, 4247, 5602, 6800
SmoI CTYRAG 9 cut(s) 2086, 2462, 2841, 3047, 3142, 4111, 4247, 5602, 6800
SnaBI TACGTA 1 cut(s) 1202
SpeI ACTAGT 2 cut(s) 3932, 4568
SphI GCATGC 2 cut(s) 2381, 6439
SseBI AGGCCT 1 cut(s) 3656
SspI AATATT 3 cut(s) 4341, 4378, 4523
SstI GAGCTC 2 cut(s) 907, 2741
StuI AGGCCT 1 cut(s) 3656
StyI CCWWGG 7 cut(s) 165, 328, 818, 2372, 3508, 4531, 5058
TaiI ACGT 5 cut(s) 921, 1204, 1336, 2995, 6189
TaqI TCGA 8 cut(s) 1309, 3222, 5730, 6012, 6198, 6416, 6786, 6801
TaqII GACCGA 2 cut(s) 897, 5210
TatI WGTACW 7 cut(s) 52, 1867, 2078, 3623, 4194, 5401, 6293
TauI GCSGC 5 cut(s) 1790, 1916, 6068, 6504, 6782
TseFI GTSAC 7 cut(s) 16, 1387, 2322, 2621, 3446, 4284, 5869
Tsp45I GTSAC 7 cut(s) 16, 1387, 2322, 2621, 3446, 4284, 5869
TspGWI ACGGA 5 cut(s) 737, 3091, 5344, 5849, 5901
Tth111I GACNNNGTC 1 cut(s) 672
Van91I CCANNNNNTGG 2 cut(s) 2023, 4654
Vha464I CTTAAG 1 cut(s) 5602
VneI GTGCAC 3 cut(s) 1335, 3104, 5080
VpaK11BI GGWCC 6 cut(s) 668, 830, 2123, 2957, 4012, 6344
VspI ATTAAT 2 cut(s) 1970, 4203
XbaI TCTAGA 2 cut(s) 311, 1165
XceI RCATGY 7 cut(s) 982, 1780, 2085, 2381, 3253, 6439, 6519
XcmI CCANNNNNNNNNTGG 2 cut(s) 3644, 6218
XhoI CTCGAG 1 cut(s) 6800
XmiI GTMKAC 2 cut(s) 1875, 6198
XmnI GAANNNNTTC 1 cut(s) 467
ZrmI AGTACT 2 cut(s) 4196, 6295
Zsp2I ATGCAT 4 cut(s) 757, 984, 2116, 3322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.