Rw2G046810

HEAT repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002980
Physical Location & Seq
Reverse (-)
74183915 .. 74204987
21073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G046810.1

Sequence Viewer

Length: 5487 bp
ATGATAGAGCATGTACGGATTGAGGCTGCTTTGACATTGCGTGCACTGGCTGAGGTTGATCCCACCTGTGTTGGTGGTTTAATTTCATATGGGGTGACCATGTTGAATGCTCTGAGAGAAAATATATCGTTTGAAAAGGGGAGCACTTTGCAATTAGAGCTCGATTCTTTGCATGGGCAAGCTACTGTGTTAGCAACTCTGGTGTCCATTTCCCCAAAATTACCTCTTGGTTATCCCGCTAGGTTGCCCAAATCAGTTCTTGAAGTTTCAAAGAAAATGCTGACTGAATCTAGTCGGAATCCTCTGGCCGCTACAATTGAAAAAGAAGCTGGGTGGTTACTTTTGTCATCTCTCTTAGCATCCATGCCGAAGGAGGAGCTTGAAGACCAGGTGTTTGATATTCTTTCCTTGTGGGTGTCCCTTTTTACTGGAAACCCACAAAATGAAACTAACCAAACTGGGGATTTGATATCTAGGATTCGTATGTGGTCTGCTGCAATTGATGCACTTACGTCATTTTTAAGATGCTTTCTTTCCCAAGATGCTAAGAATAATCGGATTTTGCTTCAACCTGTGCTTGTATACCTAAGTAGGGCTTTATCCTTTATATCACTGATAGCGGCCAAGGAACTGCCAAATGTGAAGCCTGCTCTGAATATATATATTATCAGGACACTAATAGCTTATCAGTCTCTTCCTGATCCAATGGCATATAAGAATGAACACCCCCAAATTATTCAAATTTGCACTAGTCCATTCAGAGAGGCTTTTGGGTGTGAAGAAAGTTCATGCTTGAGGTTTCTGTTAGACAAAAGAGATGCATGGTTAGGTCCCTGGATTCCTGGAAGGGATTGGTTTGAAGATGAACTTCGTGCTTTTCAAGGTGGAAAAGATGGTATTATGCCCTGTGTCTGGGAGAACGAAGTTTCCAGCTTTCCTCAGCCGGAACCAGTAAACAAGATATTGGTGAACCAGATGCTTCTCTGCTTTGGAGTCATGTTTGCTTCTCAGGATAGTGGTGGGATGCTGTCACTTCTTGGAATGATTGAGCAATCTATGAAAGCTGGAAGAAAGCAACCATGGCATGCAGCTAGTGTAACCAATATATGTGTGGGGTTACTTTCGGGATTCAAGGCTTTGCTTTCCTTGCGTTCCCAGCCATTAGCAGTTGATGTATTAAATTCAGCACAGGCTATATTTCAGAGTATTCTAGCAGAGGGTGACATCTGTCCATCACAACGTAGGGCTGCATCAGAATGTCTTGGCCTTTTAGCTCGTCTTGGAAATGATATCTTTACAGCAAGAATGACAAGATCACTGCTTGGTGACCTAACTGGAGCAACAGATTCAAATTATGCAGGCTCAATTGCTTTTGCTCTGGGTTGCATTCATTGCAGTGCAGGAGGAATGGCATTGTCGACATTAGTGCCTTCAACTGTGAGCTCAATTTCTTTGCTTGCTAAAAGTTCAATAGCTGGTTTACAGATCTGGTCTTTACATGGACTTCTGTTAACCATAGAAGCTGCTGGCTTGTCCTATGTATCTCAAGTTCAGGCAACACTTGGCCTTGCTTTGGATATTCTTTTGTCTGAGGAGAATGGTTGGGTTGTACTTCAGCAAGGGGTTGGCCGTCTTATAAATGCTATTGTAGCTGTTCTTGGTCCTGAACTTGCCCCTGGAAGTATTTTCTTTTCACGTTGCAAGTCAGTTGTTTCAGAGATAAGCTCAGGCCAAGAAACAGCAACAATGCTCGAGAGTGTTCGCTTTACTCAACAACTTGTACTATTTGCACCACAAGCCGTTTCTGTGCACACTCATGTCCAAACTCTTCTTCCTACTTTGTCCTCAAGACAGCCAGTTTTGAGGCATCTTGCTGTTTCCACTCTTCGGCATCTTATTGAGAAAGATCCTGTGTCCGTTGTTAACGAGCAAATAGAAGATAAACTATTTCAAATGCTGGATGAAGAAACTGATTCCGAGATTGGTGATCTGGTGCGGGCCACAATTATGCGATTGCTCTATGCATCAAGCCCTTCACATCCATCTCATTGGATGTCAATATGTCGTAGTGTGGTTCTTGCCACATCAATGAGGAGAAATGCTGATGGTACAAACGGTTTAGAAAATGATCCGGTGGGTACAGAGGGTGATCCAAGTTTAAATTCTGGAGAAGATGATGATAACATGGTTTCTGGATCTAGAGGCTCACCCCAGTTGATTCTCAGCAGGGAAAAGCACCTCAGATACCGAACCAGAGTGTTTGCTGCTGAGTGTTTGAGTTACCTACCTGGAGCTGTTGGAAAGAATCCAGCTCATTTTGATCTCTGTTTGGCTAGGGACCAATCTACTAATGGACGGGCCTCTGGTGATTGGCTTGTCCTCCATATCCAAGAACTTATAGCACTTGCTTATCAGATAAGCACGATCCAGTTTGAAAACATGCAGCCAATTGGTGTAGTGCTTCTGAGTACGATTACAGACAAGTTCGAGAGGACTCCTGACCCGGAGCTTCCAGGGCACCTCCTGCTGGAACAATATCAGGCGCAGCTAGTGTCTGCTGTCCGCACTGCCTTAGACTCGTCCTCTGGTCCTATTCTTTTGGAAGCAGGCTTCCAGTTGGCTACAAAGATATTCACAAGCGGAATAATTAAGGGTGATCAGATTGCTGTTAAGCGCATATATTCTTTGATTTCGCGACCATTGAATGACTTCAAGGACCTGTATTATCCTTCATTTGCAGAATGGGTCTCGTGCAAGATTAAGATAAGACTTCTTGCTGCCCATGCCTCTCTCAAATGTTATACATATGCATTCTTGAGGAGACACCAAAGTGCGGTTCCAGATGAATATTTAGCACTTTTACCATTATTTTCAAAGAGTTCAAACATGCTTGGGAAGTACTGGATTAGAGTGTTGAAGGACTACAGCTATATTTGCTTGTGCGTGCATCTTAAGGCAAAGTGGAATCCTTTTCTTGATGGAATTCAATCACCTCTGGTATCATCAAAGTTGGAGACCTGCTTGGAAGAATCCTGGCCAGTAATATTGCAGGCAATTGCATTGGATGCAGTTCCAGTGAATCTTGAAGAGAATGAATCTTCAAAATCCACTGATGAAACTACATCAAGTAATAGCTTATTGTCTGGATATAGTATGGTTCAATTGGAATCCGAAGATTATCAGTTTCTCTGGGGATTTGCTTTGCTAGTTTTATTTCAGGGGCAAAATTCGACCCCCAGTGGATTGAAAAATCCAGTTTCTTTTGTTAAAGCTTATAACAGGGGTGATCCATCCAGTGAAGAATTATCTTCCCCAGGAGTTAATTTATACGAGATTGTATTACCGGTCTTCCAGTTTTTGTCGACCAAAAGATTTGCCAGTGCAGGATTTCTCACTATGGACATATGCAGTGAATTGCTTCAGGTTTTCTCATATTCTATGTGCATGGATAATTCATGGGATACTCTTTCAGTGTCTGTTCTATCACAGATTGTGAAGAACTGCCCTGAAACATTTTATGAACCGGAAAACTTTGCTTATCTGGCAATGGAACTCTGTTTGGCGTACCTCTGCAAAGTGTTTCTAAGTACCGATGTGACATCTCTGGACAAATCCTTGGAGGATTTGATATCCTCAATATTTGTCACAGCAAAAACCCTTGTGAACTGTTTTCAACCAAAGAAGCAACTAGTATCTGCGGCATTGGCGTTTGTTTTAATTGGTTACAAGGGAATCAGAGAAGTCTCAACTGACTTCTGCTTCTCCAAACTTGATGAATATTTCAAGTGCACGAGTCTCTTATTGAAAAGATTTATTGATGATACATGCAGTGTTGGTGATGATGGTATTCTTCAGATGAGAAAAATATTGGGAACTTGTCTGAATGTGATCACTGGTTTAACCAAGGACTGCATTGATTGTATTCATATGCTGGAGAATAAGAGATCTGATTCGCATACATTACTGCAAACAAAGCTTGCTTTCTCTCTTGAACAAACTATATCATTTGCCAAGCTGGGTTATGAAATGGATTATCTTGAAGATAATACAGATGGTGATTCAATTTATTATGGTATGTTTAAATATTGCACCAGATGTGTCCAAACTGTACTCACCGATTCAAGTCTACAGGTTCAAGAAATTGGGTTGCAAGTACTAAAAAACTTGATTCAGAAAGGCACAGATGCAGAGGATGACACTTTCTTAATGCTTTTTGTTGGGGAACTTGCTTGTGATTTTTTCCTGATAATGCAGAATATGCTAAAGAAACCTGTAACTGAAAAATCAGCATCTGTTGCTGGTGAATGTTTAAGACTTTTAGTGCTTCTGCAAACATCGTCAAAATATAGCGAATGTCAGAGAGGTTTTATGAATTTGCTCCTGGAAGCTGTTCTCGTGGTTTTCAAGGCTTCAGAAGAAGGTTCTTCTCAGGAAGTCAATAAACTTAGGAGCATTGCGGTGAGACTTGTTTCTCATCTTGCTCAAGTTCCTTCCTCAGCAGTTCACTTCAAGGATGTGTTGCTGTCTATGCCTCTGACTCACCGACAGCAATTTCAGGGGTTTATTCGTGCTTCTGTGACCCAAGAGCATAATGCAACACAGATGAAGCCCACAACTCCATTTTTGGAGATAAAATTACCAGTGCCAACAGTGGTAAGTAAAGAGAAACAGCCTCCACTACCAGCCACTACATCACATTCTTCTGTTTCTGATTATGAAAGAGGTGGAGAGGAGGAAGAGGATGAAGATGAAGATGATTGGGATGCTTTTCAGTCTTTTCCTGCTACTACAAATGCAGCTGAAATTGACTCGAGAGTAGACAGTGCCTTGGAAACACCCAACCTGGTTGAGAAATTGTCTCCTTCGGAAGTGAATACAGAGAGTGATCAATTTCATGGAGATTCTATTTCCCAACCTCTCAACAATGTAGATGCTACCAGCAAGGCAGACCATCAAGAGGCTGGCGAAGCTGAGGTGATATCTGATTCCCCGGATGATCTGAGATCCTCACAGGGTAATATACTACGTGACAATGTCAAGACAGAAAAACCCGATGATTCCCAAACTAATAGTGGTGTGATCGAGGCATTGGATGATCAAAAGGAGAGAGACGATAAATTGTTATCCGGACCAGAAGAGGGAATGGGAGCAGAGTTGAACCAAGTTACTGAACACAGACCATCTGAACTTCATCCTATTGAAGATGCTGAAGGATTGGTCGGAAAGAATTCAGCACACCATGAGCACGGAAAGGAGAACACTGATAATAGACCAGTGCAATCATCATCAGATCCTGTAAAGCTTGGTGAGGAAGTAAAGGGAGGAGGGTCGAACCAAGTGACAGAACAGATACCATCTGAACTTCATCCTGTCGAAGATGCTCAGGGATCGGTTGCTGTGAATTCAGCGCACCATGAGCAGGGAAAGGAGATCCCTGATAATAGACCAGTGCAATCATCATCAGATCCTGTAAAGCTTGACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1828

Amino Acids

201.2

Weight (kDa)

4.98

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Laa1_Sip1_HTR5 PF20210 791 - 976 3.1e-43 Laa1/Sip1/HEATR5 HEAT repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1637, 3276
Acc36I ACCTGC 1 cut(s) 3026
AccB1I GGYRCC 1 cut(s) 2517
AccI GTMKAC 5 cut(s) 582, 1418, 3362, 4126, 4818
AccII CGCG 1 cut(s) 2695
AccIII TCCGGA 1 cut(s) 5126
AciI CCGC 9 cut(s) 237, 309, 620, 1996, 2563, 2640, 2834, 3698, 4457
AcoI YGGCCR 4 cut(s) 306, 621, 1627, 3035
AcsI RAATTY 8 cut(s) 741, 1180, 2161, 2982, 3226, 4372, 5227, 5401
AcuI CTGAAG 5 cut(s) 1598, 3404, 3836, 4395, 5229
AflII CTTAAG 1 cut(s) 2951
AgeI ACCGGT 1 cut(s) 3343
AhlI ACTAGT 2 cut(s) 749, 3688
AjuI GAANNNNNNNTTGG 6 cut(s) 1815, 1847, 2146, 2178, 2820, 2852
AleI CACNNNNGTG 1 cut(s) 2829
AloI GAACNNNNNNTCC 2 cut(s) 911, 943
Alw21I GWGCWC 7 cut(s) 46, 146, 162, 1445, 1812, 3791, 5247
Alw26I GTCTC 9 cut(s) 696, 2752, 2815, 3008, 3748, 3800, 4456, 4863, 5103
Alw44I GTGCAC 3 cut(s) 42, 1808, 3787
AlwNI CAGNNNCTG 4 cut(s) 3476, 4292, 5294, 5468
Ama87I CYCGRG 2 cut(s) 1751, 4810
Aor13HI TCCGGA 1 cut(s) 5126
AoxI GGCC 9 cut(s) 306, 621, 1264, 1564, 1627, 1729, 1998, 2358, 3035
ApaLI GTGCAC 3 cut(s) 42, 1808, 3787
ApoI RAATTY 8 cut(s) 741, 1180, 2161, 2982, 3226, 4372, 5227, 5401
ArsI GACNNNNNNTTYG 2 cut(s) 377, 409
AsiGI ACCGGT 1 cut(s) 3343
Asp700I GAANNNNTTC 4 cut(s) 2708, 3417, 4389, 5227
AspLEI GCGC 3 cut(s) 2545, 2676, 5410
AspS9I GGNCC 8 cut(s) 830, 1661, 1998, 2338, 2358, 2588, 2716, 5129
AsuC2I CCSGG 2 cut(s) 2504, 4991
AvaI CYCGRG 2 cut(s) 1751, 4810
AvaII GGWCC 6 cut(s) 830, 1661, 2338, 2588, 2716, 5129
BaeGI GKGCMC 4 cut(s) 46, 1812, 2520, 3791
BalI TGGCCA 1 cut(s) 3037
BanI GGYRCC 1 cut(s) 2517
BanII GRGCYC 2 cut(s) 162, 1445
BauI CACGAG 3 cut(s) 2749, 3790, 4394
BbsI GAAGAC 2 cut(s) 390, 3340
Bbv12I GWGCWC 7 cut(s) 46, 146, 162, 1445, 1812, 3791, 5247
BbvCI CCTCAGC 4 cut(s) 51, 939, 4495, 4971
BceAI ACGGC 2 cut(s) 1614, 1784
BcgI CGANNNNNNTGC 2 cut(s) 1408, 1442
BciVI GTATCC 1 cut(s) 3454
BclI TGATCA 4 cut(s) 2656, 3888, 4885, 5095
BcnI CCSGG 2 cut(s) 2504, 4991
BcoDI GTCTC 9 cut(s) 696, 2752, 2815, 3008, 3748, 3800, 4456, 4863, 5103
BcuI ACTAGT 2 cut(s) 749, 3688
BfmI CTRYAG 2 cut(s) 2923, 4127
BfrI CTTAAG 1 cut(s) 2951
BfuAI ACCTGC 1 cut(s) 3026
BfuI GTATCC 1 cut(s) 3454
BglII AGATCT 2 cut(s) 1485, 3944
BmcAI AGTACT 2 cut(s) 2900, 4155
Bme18I GGWCC 6 cut(s) 830, 1661, 2338, 2588, 2716, 5129
BmeT110I CYCGRG 2 cut(s) 1751, 4810
BmgT120I GGNCC 8 cut(s) 830, 1661, 1998, 2338, 2358, 2588, 2716, 5129
BmiI GGNNCC 5 cut(s) 832, 948, 2339, 2519, 2838
BmrI ACTGGG 3 cut(s) 468, 2206, 3231
BmuI ACTGGG 3 cut(s) 468, 2206, 3231
BoxI GACNNNNGTC 1 cut(s) 1227
BpiI GAAGAC 2 cut(s) 390, 3340
BplI GAGNNNNNCTC 2 cut(s) 1709, 1741
BpmI CTGGAG 4 cut(s) 1356, 2187, 2310, 3953
Bpu10I CCTNAGC 6 cut(s) 51, 939, 1726, 4495, 4971, 5382
BpuEI CTTGAG 5 cut(s) 814, 1530, 1831, 2836, 4467
BpuMI CCSGG 2 cut(s) 2504, 4991
BsaAI YACGTR 1 cut(s) 5027
BsaI GGTCTC 2 cut(s) 2752, 3008
BsaWI WCCGGW 4 cut(s) 2131, 3343, 3523, 5126
Bse118I RCCGGY 1 cut(s) 3343
Bse3DI GCAATG 4 cut(s) 35, 1390, 3552, 4452
BseAI TCCGGA 1 cut(s) 5126
BseMI GCAATG 4 cut(s) 35, 1390, 3552, 4452
BseRI GAGGAG 6 cut(s) 389, 1607, 2107, 2833, 4745, 5337
BseSI GKGCMC 4 cut(s) 46, 1812, 2520, 3791
BseYI CCCAGC 3 cut(s) 329, 1155, 4015
BsgI GTGCAG 2 cut(s) 1419, 3402
Bsh1236I CGCG 1 cut(s) 2695
BshFI GGCC 9 cut(s) 308, 623, 1266, 1566, 1629, 1731, 2000, 2360, 3037
BshNI GGYRCC 1 cut(s) 2517
BshTI ACCGGT 1 cut(s) 3343
BsiHKAI GWGCWC 7 cut(s) 46, 146, 162, 1445, 1812, 3791, 5247
BsiHKCI CYCGRG 2 cut(s) 1751, 4810
BsiSI CCGG 7 cut(s) 944, 2132, 2504, 3344, 3524, 4991, 5127
BslFI GGGAC 3 cut(s) 403, 816, 2351
BsmAI GTCTC 9 cut(s) 696, 2752, 2815, 3008, 3748, 3800, 4456, 4863, 5103
BsmBI CGTCTC 1 cut(s) 5103
BsmFI GGGAC 3 cut(s) 403, 816, 2351
BsmI GAATGC 3 cut(s) 112, 1386, 2810
BsnI GGCC 9 cut(s) 308, 623, 1266, 1566, 1629, 1731, 2000, 2360, 3037
Bso31I GGTCTC 2 cut(s) 2752, 3008
BsoBI CYCGRG 2 cut(s) 1751, 4810
Bsp1286I GDGCHC 8 cut(s) 46, 146, 162, 1445, 1812, 2520, 3791, 5247
Bsp13I TCCGGA 1 cut(s) 5126
Bsp19I CCATGG 1 cut(s) 1079
Bsp68I TCGCGA 1 cut(s) 2695
BspACI CCGC 9 cut(s) 237, 309, 620, 1996, 2563, 2640, 2834, 3698, 4457
BspANI GGCC 9 cut(s) 308, 623, 1266, 1566, 1629, 1731, 2000, 2360, 3037
BspEI TCCGGA 1 cut(s) 5126
BspFNI CGCG 1 cut(s) 2695
BspLI GGNNCC 5 cut(s) 832, 948, 2339, 2519, 2838
BspMI ACCTGC 1 cut(s) 3026
BspT107I GGYRCC 1 cut(s) 2517
BspTI CTTAAG 1 cut(s) 2951
BspTNI GGTCTC 2 cut(s) 2752, 3008
BsrDI GCAATG 4 cut(s) 35, 1390, 3552, 4452
BsrFI RCCGGY 1 cut(s) 3343
BssAI RCCGGY 1 cut(s) 3343
BssNAI GTATAC 1 cut(s) 583
BssSI CACGAG 3 cut(s) 2749, 3790, 4394
BssT1I CCWWGG 5 cut(s) 624, 1079, 3615, 3903, 4827
Bst1107I GTATAC 1 cut(s) 583
Bst2BI CACGAG 3 cut(s) 2749, 3790, 4394
Bst4CI ACNGT 7 cut(s) 187, 1438, 2117, 3668, 4108, 4651, 4823
Bst6I CTCTTC 6 cut(s) 699, 1833, 1890, 3081, 4731, 5130
BstAFI CTTAAG 1 cut(s) 2951
BstAPI GCANNNNNTGC 6 cut(s) 503, 1392, 2524, 3065, 4258, 4295
BstBAI YACGTR 1 cut(s) 5027
BstDSI CCRYGG 1 cut(s) 1079
BstEII GGTNACC 2 cut(s) 94, 1325
BstFNI CGCG 1 cut(s) 2695
BstHHI GCGC 3 cut(s) 2545, 2676, 5410
BstMAI GTCTC 9 cut(s) 696, 2752, 2815, 3008, 3748, 3800, 4456, 4863, 5103
BstNSI RCATGY 5 cut(s) 14, 1088, 2443, 2890, 3828
BstPAI GACNNNNGTC 1 cut(s) 1227
BstPI GGTNACC 2 cut(s) 94, 1325
BstSFI CTRYAG 2 cut(s) 2923, 4127
BstSLI GKGCMC 4 cut(s) 46, 1812, 2520, 3791
BstUI CGCG 1 cut(s) 2695
BstV2I GAAGAC 2 cut(s) 390, 3340
BstX2I RGATCY 8 cut(s) 1485, 1906, 2195, 3944, 5003, 5290, 5430, 5464
BstXI CCANNNNNNTGG 1 cut(s) 2049
BstYI RGATCY 8 cut(s) 1485, 1906, 2195, 3944, 5003, 5290, 5430, 5464
BstZ17I GTATAC 1 cut(s) 583
BsuI GTATCC 1 cut(s) 3454
BsuRI GGCC 9 cut(s) 308, 623, 1266, 1566, 1629, 1731, 2000, 2360, 3037
BtgI CCRYGG 1 cut(s) 1079
BtsI GCAGTG 5 cut(s) 1316, 1402, 2565, 3415, 3835
BtuMI TCGCGA 1 cut(s) 2695
BveI ACCTGC 1 cut(s) 3026
CaiI CAGNNNCTG 4 cut(s) 3476, 4292, 5294, 5468
CfoI GCGC 3 cut(s) 2545, 2676, 5410
Cfr10I RCCGGY 1 cut(s) 3343
Cfr13I GGNCC 8 cut(s) 830, 1661, 1998, 2338, 2358, 2588, 2716, 5129
CsiI ACCWGGT 2 cut(s) 387, 4842
CspAI ACCGGT 1 cut(s) 3343
CspCI CAANNNNNGTGG 2 cut(s) 52, 87
DraI TTTAAA 2 cut(s) 2160, 4081
EaeI YGGCCR 4 cut(s) 306, 621, 1627, 3035
Eam1104I CTCTTC 6 cut(s) 699, 1833, 1890, 3081, 4731, 5130
EarI CTCTTC 6 cut(s) 699, 1833, 1890, 3081, 4731, 5130
Ecl136II GAGCTC 2 cut(s) 160, 1443
Eco130I CCWWGG 5 cut(s) 624, 1079, 3615, 3903, 4827
Eco24I GRGCYC 2 cut(s) 162, 1445
Eco31I GGTCTC 2 cut(s) 2752, 3008
Eco32I GATATC 4 cut(s) 471, 1291, 3630, 4980
Eco47I GGWCC 6 cut(s) 830, 1661, 2338, 2588, 2716, 5129
Eco53kI GAGCTC 2 cut(s) 160, 1443
Eco57I CTGAAG 5 cut(s) 1598, 3404, 3836, 4395, 5229
Eco88I CYCGRG 2 cut(s) 1751, 4810
Eco91I GGTNACC 2 cut(s) 94, 1325
EcoICRI GAGCTC 2 cut(s) 160, 1443
EcoO109I RGGNCCY 2 cut(s) 830, 2716
EcoO65I GGTNACC 2 cut(s) 94, 1325
EcoRI GAATTC 3 cut(s) 2982, 5227, 5401
EcoRV GATATC 4 cut(s) 471, 1291, 3630, 4980
EcoT14I CCWWGG 5 cut(s) 624, 1079, 3615, 3903, 4827
EcoT22I ATGCAT 3 cut(s) 823, 2026, 2812
EcoT38I GRGCYC 2 cut(s) 162, 1445
ErhI CCWWGG 5 cut(s) 624, 1079, 3615, 3903, 4827
Esp3I CGTCTC 1 cut(s) 5103
FalI AAGNNNNNCTT 4 cut(s) 580, 612, 852, 884
FaqI GGGAC 3 cut(s) 403, 816, 2351
FauI CCCGC 2 cut(s) 244, 1989
FauNDI CATATG 4 cut(s) 88, 2806, 3404, 3927
FbaI TGATCA 4 cut(s) 2656, 3888, 4885, 5095
FblI GTMKAC 5 cut(s) 582, 1418, 3362, 4126, 4818
FriOI GRGCYC 2 cut(s) 162, 1445
GlaI GCGC 3 cut(s) 2544, 2675, 5409
GsaI CCCAGC 3 cut(s) 333, 1159, 4019
GsuI CTGGAG 4 cut(s) 1356, 2187, 2310, 3953
HaeIII GGCC 9 cut(s) 308, 623, 1266, 1566, 1629, 1731, 2000, 2360, 3037
HapII CCGG 7 cut(s) 944, 2132, 2504, 3344, 3524, 4991, 5127
HhaI GCGC 3 cut(s) 2545, 2676, 5410
Hin6I GCGC 3 cut(s) 2543, 2674, 5408
HinP1I GCGC 3 cut(s) 2543, 2674, 5408
HincII GTYRAC 4 cut(s) 1419, 1512, 1924, 3363
HindII GTYRAC 4 cut(s) 1419, 1512, 1924, 3363
HindIII AAGCTT 4 cut(s) 3270, 3974, 5300, 5474
HpaI GTTAAC 2 cut(s) 1512, 1924
HpaII CCGG 7 cut(s) 944, 2132, 2504, 3344, 3524, 4991, 5127
HpyCH4III ACNGT 7 cut(s) 187, 1438, 2117, 3668, 4108, 4651, 4823
HpyCH4IV ACGT 4 cut(s) 512, 1240, 1696, 5026
HpySE526I ACGT 4 cut(s) 512, 1240, 1696, 5026
HspAI GCGC 3 cut(s) 2543, 2674, 5408
Kpn2I TCCGGA 1 cut(s) 5126
Ksp22I TGATCA 4 cut(s) 2656, 3888, 4885, 5095
KspAI GTTAAC 2 cut(s) 1512, 1924
LmnI GCTCC 8 cut(s) 141, 376, 1337, 2291, 2506, 4383, 4449, 5147
MabI ACCWGGT 2 cut(s) 387, 4842
MaeII ACGT 4 cut(s) 512, 1240, 1696, 5026
MfeI CAATTG 6 cut(s) 315, 498, 1365, 2448, 3054, 3161
MflI RGATCY 8 cut(s) 1485, 1906, 2195, 3944, 5003, 5290, 5430, 5464
MhlI GDGCHC 8 cut(s) 46, 146, 162, 1445, 1812, 2520, 3791, 5247
MlsI TGGCCA 1 cut(s) 3037
MluNI TGGCCA 1 cut(s) 3037
MlyI GAGTC 6 cut(s) 1002, 2488, 2570, 3802, 4531, 4802
MmeI TCCRAC 4 cut(s) 275, 2278, 2991, 5200
Mox20I TGGCCA 1 cut(s) 3037
Mph1103I ATGCAT 3 cut(s) 823, 2026, 2812
MroI TCCGGA 1 cut(s) 5126
MroXI GAANNNNTTC 4 cut(s) 2708, 3417, 4389, 5227
MscI TGGCCA 1 cut(s) 3037
MslI CAYNNNNRTG 7 cut(s) 1255, 1395, 1815, 2006, 2087, 2829, 4457
Msp20I TGGCCA 1 cut(s) 3037
MspA1I CMGCKG 1 cut(s) 4799
MspCI CTTAAG 1 cut(s) 2951
MspI CCGG 7 cut(s) 944, 2132, 2504, 3344, 3524, 4991, 5127
MunI CAATTG 6 cut(s) 315, 498, 1365, 2448, 3054, 3161
Mva1269I GAATGC 3 cut(s) 112, 1386, 2810
MvnI CGCG 1 cut(s) 2695
NciI CCSGG 2 cut(s) 2504, 4991
NcoI CCATGG 1 cut(s) 1079
NdeI CATATG 4 cut(s) 88, 2806, 3404, 3927
NlaIV GGNNCC 5 cut(s) 832, 948, 2339, 2519, 2838
NmuCI GTSAC 9 cut(s) 94, 1029, 1220, 1325, 3595, 3643, 4576, 5027, 5338
NruI TCGCGA 1 cut(s) 2695
NsiI ATGCAT 3 cut(s) 823, 2026, 2812
NspI RCATGY 5 cut(s) 14, 1088, 2443, 2890, 3828
OliI CACNNNNGTG 1 cut(s) 2829
PaeI GCATGC 1 cut(s) 1088
PaeR7I CTCGAG 2 cut(s) 1751, 4810
PctI GAATGC 3 cut(s) 112, 1386, 2810
PdmI GAANNNNTTC 4 cut(s) 2708, 3417, 4389, 5227
PflFI GACNNNGTC 1 cut(s) 5033
PfoI TCCNGGA 2 cut(s) 841, 4380
PinAI ACCGGT 1 cut(s) 3343
PleI GAGTC 6 cut(s) 1001, 2488, 2570, 3801, 4531, 4802
PpsI GAGTC 6 cut(s) 1001, 2488, 2570, 3801, 4531, 4802
Ppu21I YACGTR 1 cut(s) 5027
PpuMI RGGWCCY 2 cut(s) 830, 2716
PshAI GACNNNNGTC 1 cut(s) 1227
PsiI TTATAA 2 cut(s) 1637, 3276
Psp124BI GAGCTC 2 cut(s) 162, 1445
Psp5II RGGWCCY 2 cut(s) 830, 2716
PspEI GGTNACC 2 cut(s) 94, 1325
PspFI CCCAGC 3 cut(s) 329, 1155, 4015
PspN4I GGNNCC 5 cut(s) 832, 948, 2339, 2519, 2838
PspPI GGNCC 8 cut(s) 830, 1661, 1998, 2338, 2358, 2588, 2716, 5129
PspPPI RGGWCCY 2 cut(s) 830, 2716
PsrI GAACNNNNNNTAC 2 cut(s) 1533, 1565
PstNI CAGNNNCTG 4 cut(s) 3476, 4292, 5294, 5468
PsuI RGATCY 8 cut(s) 1485, 1906, 2195, 3944, 5003, 5290, 5430, 5464
PsyI GACNNNGTC 1 cut(s) 5033
PvuII CAGCTG 1 cut(s) 4799
RruI TCGCGA 1 cut(s) 2695
RseI CAYNNNNRTG 7 cut(s) 1255, 1395, 1815, 2006, 2087, 2829, 4457
SacI GAGCTC 2 cut(s) 162, 1445
SalI GTCGAC 2 cut(s) 1417, 3361
Sau96I GGNCC 8 cut(s) 830, 1661, 1998, 2338, 2358, 2588, 2716, 5129
ScaI AGTACT 2 cut(s) 2900, 4155
SchI GAGTC 6 cut(s) 1002, 2488, 2570, 3802, 4531, 4802
SduI GDGCHC 8 cut(s) 46, 146, 162, 1445, 1812, 2520, 3791, 5247
SexAI ACCWGGT 2 cut(s) 387, 4842
SfcI CTRYAG 2 cut(s) 2923, 4127
Sfr274I CTCGAG 2 cut(s) 1751, 4810
SinI GGWCC 6 cut(s) 830, 1661, 2338, 2588, 2716, 5129
SlaI CTCGAG 2 cut(s) 1751, 4810
SmiMI CAYNNNNRTG 7 cut(s) 1255, 1395, 1815, 2006, 2087, 2829, 4457
SmlI CTYRAG 8 cut(s) 793, 1545, 1751, 1846, 2815, 2951, 4482, 4810
SmoI CTYRAG 8 cut(s) 793, 1545, 1751, 1846, 2815, 2951, 4482, 4810
SpeI ACTAGT 2 cut(s) 749, 3688
SphI GCATGC 1 cut(s) 1088
SsiI CCGC 9 cut(s) 237, 309, 620, 1996, 2563, 2640, 2834, 3698, 4457
SspI AATATT 6 cut(s) 2849, 3045, 3639, 3779, 3867, 4085
SstI GAGCTC 2 cut(s) 162, 1445
StyI CCWWGG 5 cut(s) 624, 1079, 3615, 3903, 4827
TaaI ACNGT 7 cut(s) 187, 1438, 2117, 3668, 4108, 4651, 4823
TaiI ACGT 4 cut(s) 515, 1243, 1699, 5029
TatI WGTACW 5 cut(s) 1609, 1780, 2898, 4108, 4153
TauI GCSGC 3 cut(s) 311, 623, 3701
TseFI GTSAC 9 cut(s) 94, 1029, 1220, 1325, 3595, 3643, 4576, 5027, 5338
Tsp45I GTSAC 9 cut(s) 94, 1029, 1220, 1325, 3595, 3643, 4576, 5027, 5338
TspGWI ACGGA 3 cut(s) 31, 1906, 5262
Tth111I GACNNNGTC 1 cut(s) 5033
Vha464I CTTAAG 1 cut(s) 2951
VneI GTGCAC 3 cut(s) 42, 1808, 3787
VpaK11BI GGWCC 6 cut(s) 830, 1661, 2338, 2588, 2716, 5129
XapI RAATTY 8 cut(s) 741, 1180, 2161, 2982, 3226, 4372, 5227, 5401
XbaI TCTAGA 1 cut(s) 2198
XceI RCATGY 5 cut(s) 14, 1088, 2443, 2890, 3828
XcmI CCANNNNNNNNNTGG 4 cut(s) 1108, 2348, 4958, 5069
XhoI CTCGAG 2 cut(s) 1751, 4810
XmiI GTMKAC 5 cut(s) 582, 1418, 3362, 4126, 4818
XmnI GAANNNNTTC 4 cut(s) 2708, 3417, 4389, 5227
ZrmI AGTACT 2 cut(s) 2900, 4155
Zsp2I ATGCAT 3 cut(s) 823, 2026, 2812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.