Rroxscaffold_2G00087690

HEAT repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002980
Physical Location & Seq
Forward (+)
9695185 .. 9717420
22236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00087690.1

Sequence Viewer

Length: 6804 bp
ATGGCGAAGAAGCACGTCAAAGATAACCCCCTACCGCTTTCTCAGTTTGGCGTCTTGGTTGCGCAATTGGAGTCCATAGTCGCCTCCGCCGCCCAGAAGCCACCGGAGCCCCTCCTCTGCTTCGATCTCCTCTCCGATCTCATCTCCGCCATCGCCGAAGAGCCCAAGGAGTCGATTTTGTTATGGCAGAGGCGATGCGAGGAGGCACTTTATTCACTGCTTGTACTCGGAGCTCGGCGGCCGGTGCGGCATTTGGCTTCAGTTGCAATGGCGAGGGTTATATCCAAGGGAGATAGCATTTCAATATACTCCAGAGCCAGCAGTCTCCAAGGGTTTCTTTCTGATGGGAAGAAGAGCGACCCGCAAAAAGTAGCTGGAGCTGCACAATGCTTAGGAGAATTATATCGATATTTTGGGAGGCGAATCACTTCAGGTTTACTTGAAACGACTATGATTGCCACAAAACTCATCAAGTTTCATGAGGAATTTGTGAGACAAGAGGCTTTATATATGCTTCAAAATGCTTTGGAAGGGTCTGGTGGTAGTGCTGCTACTTCAGCATACACCGAGGCATTTCGTCTCATCATGCGGTTTGCTGTTGGTGATAAATCATTTCTTGTCAGAATCGCTGCTGCAAGATGTTTGAAGGCATTTGCTATTATTGGAGGACCGGGTTTAGGGGTGGGAGAACTTGAGAATTCAGCTTCTTTCTGTGTCAAGGTCCTTGAGGATCCTGTTTCATCGGTCCGTGATGCATTTGCTGAAGCATTGGGTTCGCTGCTTGCTCTTGGAATGAATCCTGATGCACAGGTTCAACCAAGAGGGAAGGGACCTTTTCCTCCAGCAAAGAAACTAGAAGGTGGTTTGCAAAGGCATTTAGCGTTGCCTTTCACAAAAGCAAGTGGCGCTCGGTCAAAGGATGTGCAAGTTGGCATAACATTGTCTTGGGTGTTCTTCTTACAGGCAATTCGTTTGAAGTACTTGCATCCAGACAGTGAGCTTCAAAACTATGCAATTCAGGTTATGGACATGCTTCGTGCCGATACTTCTGTTGACGCCTACACTCTGGCTTGTGTTCTCTATATCCTTCGTGTTGGTGTTACCGATCAGATGACAGAACCTACTCAGAGGAGTTTTTTGGTTTTTCTTGGACAGCAGCTTATGTCTCCTGATGCCAGTCCTTCCATGAAAATTGCTGGTCTACGTACTGTCTCATATACCCTAAAAACTCTGGGAGAGGTTCCAGCTGAGTTCAAGGAAGTACTTGACAACACTGTTGTTGCAGCAGTATCTCATTCTTCTCAACTTGTACGAATTGAGGCTGCTTTGACATTGCGTGCACTGGCTGAGGTTGATCCCACCTGTGTTGGTGGTTTAATTTCATATGGGGTGACCATGTTGAATGCTCTGAGAGAAAATATATCGTTTGAAAAGGGGAGCACTTTGCAATTAGAGCTTGATTCTTTGCATGGGCAAGCTACTGTGTTAGCAACTCTGGTGTCCATTTCCCCAAAATTACCTCTTGGTTATCCCGCTAGGGCTTTGGAAGTGGATGCTATTTGTGTTATTAGGTTGCCCAAATCAGTTCTTGAAGTTTCAAAGAAGATGCTGACTGAATCTAGTCGGAATCCTCTGGCCGCTACAATTGAAAAAGAAGCTGGGTGGTTACTTTTGTCATCTCTCTTAGCATCCATGCCGAAGGAGGAGCTTGAAGACCAGGTTTTTGATATTCTTTCCTTGTGGGTGTCCCTTTTTACTGGAAACCCACAAAATGAAACTAACCAAACTGGGGATTTGATATCTAGGATTCGCATGTGGTCTGCTGCAATTGATGCACTTACGTCATTTTTAAGATGCTTTCTTTCCCATGATGCTAAGAATAATCGGATTTTGCTTCAACCTGTGCTTGTATACCTAAGTAGGGCTTTATCCTTTATATCACTGATAGCAGCCAAGGAACTGCCAAATGTGAAGCCTGCTCTGAATATATTTATTATCAGGACACTAATAGCTTATCAGTCTCTTCCCGATCCAATGTCATATAAGAATGAACACCCCCAGATTATTCAAATTTGCACTAGTCCATTCAGAGAGGCTTTTGGATGTGAAGAAAGTTCATGCTTGAGGTTTCTGTTAGACAAAAGAGATGCATGGTTAGGTCCCTGGATTCCTGGAAGGGATTGGTTTGAAGATGAACTTCGTGCTTTTCAAGGTGGAAAAGATGGTATTATGCCCTGTGTCTGGGAGAACGACGTTTCTAGCTTTCCTCAGCCGGAACCAGTAAACAAGATATTGGTGAACCAGATGCTTCTCTGCTTTGGAGTCATGTTTGCTTCTCAGGATAGTGGTGTGATGCTGTCACTTCTTGGAATGATTGAGCAATCTATGAAAGCTGGAAGAAAGCAACCATGGCATGCGGCTAGTGTAACCAATATATGTGTGGGGTTACTTTCGGGATTCAAGGCTTTGCTTTCCTTGCGTTCCCAGCCATTAGCAGTTGATGTATTAAATTCAGCACAGGCTATATTTCAGAGTATTCTAGCAGAGGGTGACATCTGTCCATCACATCGTAGGGCTGCATCAGAATGTCTTGGCCTTTTAGCTCGTCTTGGAAATGATATCTTTACAGCAAGAATGACAAGATCACTGCTTGGTGACCTAACTGGAGCAACAGATTCAAACTATGCAGGCTCAATTGCTTTTGCTCTGGGTTGCATTCATTGCAGTGCAGGAGGAATGGCATTGTCAACATTAGTGCCTTCAACTGTGAGCTCAATTTCTTTGCTTGCTAAAAGTTCAATAGCTGGTTTACAGATCTGGTCTTTACATGGACTTCTGTTAACCATAGAAGCTGCTGGCTTGTCCTATGTATCTCAAGTTCAGGCAACACTTGGCCTTGCTTTGGATATTCTTTTGTCTGAGGAGAATGGTTGGGTTGTACTTCAGCAAGGGGTTGGCCGTCTTATAAATGCTATTGTAGCTGTTCTTGGTCCTGAACTTTCCCCTGGAAGTATTTTCTTTTCACGTTGCAAGTCAGTTGTTTCAGAGATAAGCTCAGGCCAAGAAACAGCAACAATGCTTGAGAGTGTTCGCTTTACTCAACAACTTGTACTATTTGCACCACAAGCCGTTTCTGTGCACACCCATGTCCAAACTCTTCTTCCTACTTTATCCTCAAGACAGCCAGTTTTGAGGCATCTTGCTGTTTCCACTCTTCGACATCTTATTGAGAAAGATCCAGTGTCCGTTGTTAACGAGCAAATAGAAGATAAACTATTTCAAATGCTGGATGAAGAAACTGATTCCGAGATTGGTGATCTGGTGCGGGCCACAATTATGCGATTGTTCTATGCATCAAGCCCTTCACGTCCATCTCATTGGATGTCAATATGTCGTAGTGTGGTTCTTGCCACATCAATGAGGAGAAATGTTGATAGCATAAACGGTTTAGAAAATGATCCGGTGGGTACAGAGGGTGATCCAAGTTTAAATTCTGGAGAAGATGATGATAACATGGTTTCTGGATCTAGAGGCTCACCCCAGTTGATTCTCAGCAGGGAAAAGCACCTCAGATATCGAACCAGAGTGTTTGCTGCTGAGTGTTTGAGTTACCTACCTGGAGCTGTTGGAAAGAATCCAGCTCATTTTGATCTCTGTTTGGCTAGGGACCAATCTACTAATGGACGGGCCTCTGGTGATTGGCTTGTCCTCCATATCCAAGAACTTATAGCACTTGCTTATCAGATAAGCACGATCCAGTTTGAAAACATGCAGCCAATTGGTGTAGTACTTCTGAGTACAATTACAGACAAGTTTGAGAGGACTCCTGACCCGGAGCTTCCAGGGCACCTCCTGCTGGAACAATATCAGGCGCAGCTAGTGTCTGCTGTCCGCACTGCCTTAGACCCGTCCTCTGGTCCTATTCTTTTGGAAGCAGGCTTCCAGTTGGCTACAAAGATATTCACAAGCGGAATAATTAAGGGTGATCAGATTGCTGTTAAGCGCATATATTCTCTGATTTTGCGACCATTGAATGACTTCAAGGACCTGTATTATCCTTCATTTGCAGAATGGGTCTCGTGCAAGATTAAGATAAGACTTCTTGCTGCCCATGCCTCTCTCAAATGTTATACATATGCATTCTTGAGGAGACACCAAAGTGCGGTTCCAGAAGAATATTTAGCACTTTTACCATTATTTTCAAAGAGTTCAGACATGCTTGGGAAGTACTGGATTAGAGTCTTGAAGGACTACAGCTATATTTGCTTGTGTGTGCATCTTAAGGCAAAGTGGAATCCTTTTCTTGATGGAATTCAATCACCTCTGGTATCATCAAAGTTGCAGCCCTGCTTGGAAGAATCCTGGCCCGTAATATTGCAGGCAATTGCATTGGATGCAGTTCCAGTGAATCTTGAAGAGAATGAATCTTCAAAATCCACTGATGAAACTACATCAAGTAATAGCTTATTGTCTGGATATAGTATGGTTCAATTAGAATCCGAAGATTATCAGTTTCTCTGGGGATTTGCTTTGCTTGTTTTATTTCAGGGGCAAAATTCGACCCCCAGTGGATTGAAAAATCTAGTTTCTTTCGTTAAAGCTTACAACAGGGGAGATGCATCCAGTGAAGAATTATCTTCCCCAGGAGTTAATTTATACGAGATTGTATTACCGGTCTTCCAGTTTTTATCCACCAAAAGATTTGCCAGTGCAGGATTTCTCACTATGGACATATGCAGTGAATTGCTTCAGGTTTTCTCATATTCTATGTGCATGGATAACTCATGGGATACTCTTTCAGTTTCTGTTCTATCACAGATTGTGAAGAACTGCCCTGAAACATTTTATGAACCGGAAAACTTTGCTTATCTGGCAATGGAACTCTGTTTGGCGTACCTCTGCAAAGTGTTTCTAAGTACCGATGTGACATCTCTGGACAAATCCTTGGAGGATTTGATATCCTCAATATTTGTCACAGCAAAAACCCTTGTGAACAGTTTTCAACCAAAGAAGCAACTAGTATCTGCGGCATTGGCGTTTCTTTTAATTGGTTACAAGGGAATCAGAGAAGTCTCAACTGACTTCTGCTTCTCCAAACTTGATGAATATTTCAAGCGCACGAGTCTCTTATTGAAAAGATTTATTGATGATACATGCAGTGTTGGTGATGATGGTATTCTTCAGATGAGAAAAATATTGGGAACTTGTCTGAATGTGATCACTGATTTGACCAAGGACTGCATTAAGTGTATTCATATGCTGGAGAATAAGAGATCTGATTCGCATACATTACTGCAAACAAAGCTTGCTTTCTCTCTTGAACAAACTATATCATTTGCCAAGCTTGGTTATGAAATGGATTATCTTGAAGATAATACAGATGGTGATTCAATTTATTATGGTATGTTTAAATATTGCACCAGATGTGTCCAAACTGTACTCATCGATTCGAGTTTACAGGTTCAAGAAATTGGGTTGCAAGTATTAAAAAACTTGATTCAGAAAGGCACAGATGCAGAGGATGACACTTTCTTAATGCTTTTTGTTGGGGAACTTGCTTGTGATTTTTTCCTGATAATGCAGAATATGCTAAAGAAATCTGTAACTGAAAAATCAGCATCTGTTGCTGGTGAATGTTTAAGACTTTTAGTGCTTCTGCAAACATCGTCAAAATCTAGCGAATGTCAGAGAGGTTTTATGAATTTGCTCCTGGAAGCTGTTCTCGTGGTTTTCAAGGCTTCAGAAGAAGGTTCTTCTCAGGAAGTCAATAAACTTAGGAGCATTGCTGTGAGACTTGTTTCTCATCTTGCTCAAGTTCCTTCCTCAGCAGTTCACTTCAAGGATGTGTTGCTGTCTATGCCTCTGACTCACCGACAGCAATTTCAGGGGTTTATTCGTGCTTCTGTGACCCAAGAGCATAATGCAACACAGACGAAGCCCACAACTCCATTTTTGGAGATAAAATTACCAGTGCCAACAGTGGTAAGTAAAGAGAAACAGCCTCCACTACCAGCCACTACATCACATTCTTCTGTTTCTGATTATCAAAGAAATGAAGAGGAGGAAGAGGATGAAGATGAAGATGATTGGGATGCTTTTCAGTCTTTTCCTGCTACTACAAACACAGCTGAAATTGACTCGAGAGTAGACAGTGCCTTGGAAACACCCGACCTGGTTGAGAAATTGTCTCCTTCAGAAGTGAATACAGAGAGTGATCAATTTCATGGAGATTCTGTTTCCCAACCTCTCAACAATGTAGAGGCTACCAGCAAGGCAGACCATCAAGAGGCTGGCGAAGCTGAGGTGATATCTGATTCCCCGGATGATCTGACATCCTCACAGGGTAATAAACTACATGACAATGTCAAGACAGAAAAACCCAATGATTCCCAAACTAATAGTGGTGTTATCGAGGCATTGGATGATCAAAAGGAGAGAGACGATAAATTGTTATCTGGACCAGAAGAGGGAATGGGAGCAGAGTTGAACCAAGTTACTGAACACAGACCATCTGAACTTCGTCCTATGGAAGATGCTGAAGGATTGGTCGGAAAGAATTCAACACACCATGAGCAGGGAAAGGAGAACACTGATAATAGACCAGTGCAATCATCATCAGATCCTGTAAAGCTTGGTGAGGAAGTAAAGCGAGCAGGGTCAAACCAAGTGACAGAACAGATACCCTCTGAACTTCATCCTGTCGAAGATGCTCAAGGATCGGTTGCAGTGAATTCAGCGCACCATGAGCAGGGAAAGGAGATCCCTGATAATAGACCAGTGCAATCATCATCAGATCCTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

2267

Amino Acids

249.21

Weight (kDa)

5.22

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Laa1_Sip1_HTR5 PF20210 1234 - 1419 7.4e-43 Laa1/Sip1/HEATR5 HEAT repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2966
Acc16I TGCGCA 1 cut(s) 63
AccB1I GGYRCC 1 cut(s) 3846
AccI GTMKAC 3 cut(s) 1201, 1911, 6147
AcoI YGGCCR 3 cut(s) 239, 1635, 2956
AcyI GRCGYC 2 cut(s) 51, 1056
AflII CTTAAG 1 cut(s) 4280
AgeI ACCGGT 1 cut(s) 4672
AhlI ACTAGT 2 cut(s) 2078, 5017
AjiI CACGTC 2 cut(s) 16, 3368
AjuI GAANNNNNNNTTGG 6 cut(s) 3144, 3176, 3475, 3507, 4149, 4181
AleI CACNNNNGTG 1 cut(s) 4158
Alw21I GWGCWC 5 cut(s) 235, 1342, 1442, 2774, 3141
Alw44I GTGCAC 2 cut(s) 1338, 3137
AlwNI CAGNNNCTG 4 cut(s) 4805, 5621, 6623, 6797
Ama87I CYCGRG 1 cut(s) 6139
AoxI GGCC 9 cut(s) 239, 1635, 2593, 2893, 2956, 3058, 3327, 3687, 4364
ApaLI GTGCAC 2 cut(s) 1338, 3137
ArsI GACNNNNNNTTYG 2 cut(s) 1706, 1738
AsiGI ACCGGT 1 cut(s) 4672
Asp700I GAANNNNTTC 5 cut(s) 427, 4037, 4746, 5718, 6556
AspLEI GCGC 6 cut(s) 64, 908, 3874, 4005, 5118, 6739
AsuC2I CCSGG 3 cut(s) 672, 3833, 6320
AvaI CYCGRG 1 cut(s) 6139
BaeGI GKGCMC 3 cut(s) 1342, 3141, 3849
BaeI ACNNNNGTAYC 2 cut(s) 1035, 1068
BamHI GGATCC 1 cut(s) 730
BanI GGYRCC 1 cut(s) 3846
BanII GRGCYC 4 cut(s) 111, 165, 235, 2774
BauI CACGAG 3 cut(s) 4078, 5119, 5723
BbsI GAAGAC 2 cut(s) 1719, 4669
Bbv12I GWGCWC 5 cut(s) 235, 1342, 1442, 2774, 3141
BbvCI CCTCAGC 4 cut(s) 1347, 2268, 5824, 6300
BceAI ACGGC 2 cut(s) 2943, 3113
BcgI CGANNNNNNTGC 2 cut(s) 756, 790
BciVI GTATCC 1 cut(s) 4783
BclI TGATCA 4 cut(s) 3985, 5217, 6214, 6424
BcnI CCSGG 3 cut(s) 672, 3833, 6320
BcuI ACTAGT 2 cut(s) 2078, 5017
BfmI CTRYAG 1 cut(s) 4252
BfoI RGCGCY 1 cut(s) 909
BfrI CTTAAG 1 cut(s) 4280
BfuI GTATCC 1 cut(s) 4783
BglI GCCNNNNNGGC 1 cut(s) 247
BglII AGATCT 2 cut(s) 2814, 5273
BmcAI AGTACT 4 cut(s) 980, 1263, 3789, 4229
BmeT110I CYCGRG 1 cut(s) 6139
BmgBI CACGTC 2 cut(s) 16, 3368
BmiI GGNNCC 9 cut(s) 108, 732, 831, 1242, 2161, 2277, 3668, 3848, 4167
BmrI ACTGGG 3 cut(s) 1797, 3535, 4560
BmuI ACTGGG 3 cut(s) 1797, 3535, 4560
BoxI GACNNNNGTC 1 cut(s) 2556
BpiI GAAGAC 2 cut(s) 1719, 4669
BplI GAGNNNNNCTC 2 cut(s) 3038, 3070
BpmI CTGGAG 7 cut(s) 295, 396, 825, 2685, 3516, 3639, 5282
Bpu10I CCTNAGC 6 cut(s) 391, 1347, 2268, 3055, 5824, 6300
BpuEI CTTGAG 9 cut(s) 713, 746, 2143, 2859, 3101, 3160, 4165, 5796, 6696
BpuMI CCSGG 3 cut(s) 672, 3833, 6320
Bsa29I ATCGAT 2 cut(s) 406, 5445
BsaAI YACGTR 1 cut(s) 1205
BsaHI GRCGYC 2 cut(s) 51, 1056
BsaI GGTCTC 1 cut(s) 4081
BsaWI WCCGGW 4 cut(s) 103, 3460, 4672, 4852
BsaXI ACNNNNNCTCC 2 cut(s) 6219, 6249
Bse118I RCCGGY 2 cut(s) 241, 4672
Bse3DI GCAATG 5 cut(s) 273, 1331, 2719, 4881, 5781
BseCI ATCGAT 2 cut(s) 406, 5445
BseMI GCAATG 5 cut(s) 273, 1331, 2719, 4881, 5781
BseRI GAGGAG 9 cut(s) 104, 119, 215, 1144, 1718, 2936, 3436, 4162, 6074
BseSI GKGCMC 3 cut(s) 1342, 3141, 3849
BseX3I CGGCCG 1 cut(s) 239
BseYI CCCAGC 2 cut(s) 1658, 2484
BsgI GTGCAG 3 cut(s) 366, 2748, 4731
Bsh1285I CGRYCG 1 cut(s) 242
BshFI GGCC 9 cut(s) 241, 1637, 2595, 2895, 2958, 3060, 3329, 3689, 4366
BshNI GGYRCC 1 cut(s) 3846
BshTI ACCGGT 1 cut(s) 4672
BshVI ATCGAT 2 cut(s) 406, 5445
BsiEI CGRYCG 1 cut(s) 242
BsiHKAI GWGCWC 5 cut(s) 235, 1342, 1442, 2774, 3141
BsiHKCI CYCGRG 1 cut(s) 6139
BsiSI CCGG 9 cut(s) 104, 242, 671, 2273, 3461, 3833, 4673, 4853, 6320
BslFI GGGAC 4 cut(s) 843, 1732, 2145, 3680
BsmBI CGTCTC 2 cut(s) 584, 6432
BsmFI GGGAC 4 cut(s) 843, 1732, 2145, 3680
BsmI GAATGC 3 cut(s) 1408, 2715, 4139
BsnI GGCC 9 cut(s) 241, 1637, 2595, 2895, 2958, 3060, 3329, 3689, 4366
Bso31I GGTCTC 1 cut(s) 4081
BsoBI CYCGRG 1 cut(s) 6139
Bsp1286I GDGCHC 8 cut(s) 111, 165, 235, 1342, 1442, 2774, 3141, 3849
Bsp19I CCATGG 1 cut(s) 2408
BspANI GGCC 9 cut(s) 241, 1637, 2595, 2895, 2958, 3060, 3329, 3689, 4366
BspDI ATCGAT 2 cut(s) 406, 5445
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 9 cut(s) 108, 732, 831, 1242, 2161, 2277, 3668, 3848, 4167
BspQI GCTCTTC 2 cut(s) 153, 347
BspT107I GGYRCC 1 cut(s) 3846
BspTI CTTAAG 1 cut(s) 4280
BspTNI GGTCTC 1 cut(s) 4081
BsrDI GCAATG 5 cut(s) 273, 1331, 2719, 4881, 5781
BsrFI RCCGGY 2 cut(s) 241, 4672
BssAI RCCGGY 2 cut(s) 241, 4672
BssNAI GTATAC 1 cut(s) 1912
BssNI GRCGYC 2 cut(s) 51, 1056
BssSI CACGAG 3 cut(s) 4078, 5119, 5723
BssT1I CCWWGG 8 cut(s) 165, 285, 328, 1953, 2408, 4944, 5232, 6156
Bst1107I GTATAC 1 cut(s) 1912
Bst2BI CACGAG 3 cut(s) 4078, 5119, 5723
Bst6I CTCTTC 9 cut(s) 153, 347, 2028, 3162, 3219, 4410, 6051, 6060, 6459
BstACI GRCGYC 2 cut(s) 51, 1056
BstAFI CTTAAG 1 cut(s) 4280
BstAPI GCANNNNNTGC 6 cut(s) 1832, 2721, 3853, 4394, 5587, 5624
BstBAI YACGTR 1 cut(s) 1205
BstDSI CCRYGG 1 cut(s) 2408
BstEII GGTNACC 2 cut(s) 1390, 2654
BstH2I RGCGCY 1 cut(s) 909
BstHHI GCGC 6 cut(s) 64, 908, 3874, 4005, 5118, 6739
BstMCI CGRYCG 1 cut(s) 242
BstNSI RCATGY 6 cut(s) 1033, 1816, 2417, 3772, 4219, 5157
BstPAI GACNNNNGTC 1 cut(s) 2556
BstPI GGTNACC 2 cut(s) 1390, 2654
BstSFI CTRYAG 1 cut(s) 4252
BstSLI GKGCMC 3 cut(s) 1342, 3141, 3849
BstSNI TACGTA 1 cut(s) 1205
BstV2I GAAGAC 2 cut(s) 1719, 4669
BstX2I RGATCY 8 cut(s) 730, 2814, 3235, 3524, 5273, 6619, 6759, 6793
BstXI CCANNNNNNTGG 1 cut(s) 3378
BstYI RGATCY 8 cut(s) 730, 2814, 3235, 3524, 5273, 6619, 6759, 6793
BstZ17I GTATAC 1 cut(s) 1912
BstZI CGGCCG 1 cut(s) 239
Bsu15I ATCGAT 2 cut(s) 406, 5445
BsuI GTATCC 1 cut(s) 4783
BsuRI GGCC 9 cut(s) 241, 1637, 2595, 2895, 2958, 3060, 3329, 3689, 4366
BsuTUI ATCGAT 2 cut(s) 406, 5445
BtgI CCRYGG 1 cut(s) 2408
BtgZI GCGATG 2 cut(s) 136, 208
BtrI CACGTC 2 cut(s) 16, 3368
BtsI GCAGTG 7 cut(s) 215, 2645, 2731, 3894, 4744, 5164, 6732
CaiI CAGNNNCTG 4 cut(s) 4805, 5621, 6623, 6797
CciI TCATGA 1 cut(s) 478
CfoI GCGC 6 cut(s) 64, 908, 3874, 4005, 5118, 6739
Cfr10I RCCGGY 2 cut(s) 241, 4672
ClaI ATCGAT 2 cut(s) 406, 5445
CpoI CGGWCCG 1 cut(s) 745
CseI GACGC 2 cut(s) 40, 1064
CsiI ACCWGGT 2 cut(s) 1716, 6171
CspAI ACCGGT 1 cut(s) 4672
CspCI CAANNNNNGTGG 2 cut(s) 1348, 1383
CspI CGGWCCG 1 cut(s) 745
DraI TTTAAA 2 cut(s) 3489, 5410
EaeI YGGCCR 3 cut(s) 239, 1635, 2956
EagI CGGCCG 1 cut(s) 239
Eam1104I CTCTTC 9 cut(s) 153, 347, 2028, 3162, 3219, 4410, 6051, 6060, 6459
EarI CTCTTC 9 cut(s) 153, 347, 2028, 3162, 3219, 4410, 6051, 6060, 6459
EciI GGCGGA 2 cut(s) 76, 136
Ecl136II GAGCTC 2 cut(s) 233, 2772
EclXI CGGCCG 1 cut(s) 239
Eco105I TACGTA 1 cut(s) 1205
Eco130I CCWWGG 8 cut(s) 165, 285, 328, 1953, 2408, 4944, 5232, 6156
Eco24I GRGCYC 4 cut(s) 111, 165, 235, 2774
Eco31I GGTCTC 1 cut(s) 4081
Eco32I GATATC 5 cut(s) 1800, 2620, 3575, 4959, 6309
Eco52I CGGCCG 1 cut(s) 239
Eco53kI GAGCTC 2 cut(s) 233, 2772
Eco88I CYCGRG 1 cut(s) 6139
Eco91I GGTNACC 2 cut(s) 1390, 2654
EcoICRI GAGCTC 2 cut(s) 233, 2772
EcoO109I RGGNCCY 4 cut(s) 721, 830, 2159, 4045
EcoO65I GGTNACC 2 cut(s) 1390, 2654
EcoRI GAATTC 4 cut(s) 697, 4311, 6556, 6730
EcoRV GATATC 5 cut(s) 1800, 2620, 3575, 4959, 6309
EcoT14I CCWWGG 8 cut(s) 165, 285, 328, 1953, 2408, 4944, 5232, 6156
EcoT22I ATGCAT 5 cut(s) 757, 2152, 3355, 4141, 4621
EcoT38I GRGCYC 4 cut(s) 111, 165, 235, 2774
ErhI CCWWGG 8 cut(s) 165, 285, 328, 1953, 2408, 4944, 5232, 6156
Esp3I CGTCTC 2 cut(s) 584, 6432
FalI AAGNNNNNCTT 6 cut(s) 321, 353, 1909, 1941, 2181, 2213
FaqI GGGAC 4 cut(s) 843, 1732, 2145, 3680
FauI CCCGC 3 cut(s) 369, 1540, 3318
FauNDI CATATG 4 cut(s) 1384, 4135, 4733, 5256
FbaI TGATCA 4 cut(s) 3985, 5217, 6214, 6424
FblI GTMKAC 3 cut(s) 1201, 1911, 6147
FriOI GRGCYC 4 cut(s) 111, 165, 235, 2774
FspI TGCGCA 1 cut(s) 63
GlaI GCGC 6 cut(s) 63, 907, 3873, 4004, 5117, 6738
GsaI CCCAGC 2 cut(s) 1662, 2488
GsuI CTGGAG 7 cut(s) 295, 396, 825, 2685, 3516, 3639, 5282
HaeII RGCGCY 1 cut(s) 909
HaeIII GGCC 9 cut(s) 241, 1637, 2595, 2895, 2958, 3060, 3329, 3689, 4366
HapII CCGG 9 cut(s) 104, 242, 671, 2273, 3461, 3833, 4673, 4853, 6320
HgaI GACGC 2 cut(s) 40, 1064
HhaI GCGC 6 cut(s) 64, 908, 3874, 4005, 5118, 6739
Hin1I GRCGYC 2 cut(s) 51, 1056
Hin6I GCGC 6 cut(s) 62, 906, 3872, 4003, 5116, 6737
HinP1I GCGC 6 cut(s) 62, 906, 3872, 4003, 5116, 6737
HincII GTYRAC 4 cut(s) 1054, 2748, 2841, 3253
HindII GTYRAC 4 cut(s) 1054, 2748, 2841, 3253
HindIII AAGCTT 4 cut(s) 4599, 5303, 5342, 6629
HpaI GTTAAC 2 cut(s) 2841, 3253
HpaII CCGG 9 cut(s) 104, 242, 671, 2273, 3461, 3833, 4673, 4853, 6320
Hpy99I CGWCG 1 cut(s) 2255
HpyCH4IV ACGT 6 cut(s) 15, 1204, 1841, 2253, 3025, 3367
HpySE526I ACGT 6 cut(s) 15, 1204, 1841, 2253, 3025, 3367
Hsp92I GRCGYC 2 cut(s) 51, 1056
HspAI GCGC 6 cut(s) 62, 906, 3872, 4003, 5116, 6737
Ksp22I TGATCA 4 cut(s) 3985, 5217, 6214, 6424
KspAI GTTAAC 2 cut(s) 2841, 3253
LguI GCTCTTC 2 cut(s) 153, 347
MabI ACCWGGT 2 cut(s) 1716, 6171
MaeII ACGT 6 cut(s) 15, 1204, 1841, 2253, 3025, 3367
MfeI CAATTG 6 cut(s) 65, 1644, 1827, 2694, 3777, 4383
MflI RGATCY 8 cut(s) 730, 2814, 3235, 3524, 5273, 6619, 6759, 6793
MhlI GDGCHC 8 cut(s) 111, 165, 235, 1342, 1442, 2774, 3141, 3849
MlyI GAGTC 8 cut(s) 80, 179, 2331, 3817, 4248, 5131, 5860, 6131
MmeI TCCRAC 3 cut(s) 1604, 3607, 6529
Mph1103I ATGCAT 5 cut(s) 757, 2152, 3355, 4141, 4621
MroXI GAANNNNTTC 5 cut(s) 427, 4037, 4746, 5718, 6556
MslI CAYNNNNRTG 8 cut(s) 2584, 2724, 3144, 3335, 3416, 4158, 5786, 6360
MspA1I CMGCKG 2 cut(s) 1247, 6128
MspCI CTTAAG 1 cut(s) 4280
MspI CCGG 9 cut(s) 104, 242, 671, 2273, 3461, 3833, 4673, 4853, 6320
MunI CAATTG 6 cut(s) 65, 1644, 1827, 2694, 3777, 4383
Mva1269I GAATGC 3 cut(s) 1408, 2715, 4139
NciI CCSGG 3 cut(s) 672, 3833, 6320
NcoI CCATGG 1 cut(s) 2408
NdeI CATATG 4 cut(s) 1384, 4135, 4733, 5256
NlaIV GGNNCC 9 cut(s) 108, 732, 831, 1242, 2161, 2277, 3668, 3848, 4167
NmeAIII GCCGAG 1 cut(s) 214
NmuCI GTSAC 8 cut(s) 1390, 2358, 2549, 2654, 4924, 4972, 5905, 6667
NsbI TGCGCA 1 cut(s) 63
NsiI ATGCAT 5 cut(s) 757, 2152, 3355, 4141, 4621
NspI RCATGY 6 cut(s) 1033, 1816, 2417, 3772, 4219, 5157
OliI CACNNNNGTG 1 cut(s) 4158
PaeI GCATGC 1 cut(s) 2417
PaeR7I CTCGAG 1 cut(s) 6139
PagI TCATGA 1 cut(s) 478
PciSI GCTCTTC 2 cut(s) 153, 347
PctI GAATGC 3 cut(s) 1408, 2715, 4139
PdmI GAANNNNTTC 5 cut(s) 427, 4037, 4746, 5718, 6556
PflFI GACNNNGTC 1 cut(s) 6362
PfoI TCCNGGA 2 cut(s) 2170, 5709
PinAI ACCGGT 1 cut(s) 4672
PleI GAGTC 8 cut(s) 79, 178, 2330, 3817, 4247, 5130, 5860, 6131
PpsI GAGTC 8 cut(s) 79, 178, 2330, 3817, 4247, 5130, 5860, 6131
Ppu21I YACGTR 1 cut(s) 1205
PpuMI RGGWCCY 4 cut(s) 721, 830, 2159, 4045
PshAI GACNNNNGTC 1 cut(s) 2556
PsiI TTATAA 1 cut(s) 2966
Psp124BI GAGCTC 2 cut(s) 235, 2774
Psp5II RGGWCCY 4 cut(s) 721, 830, 2159, 4045
PspEI GGTNACC 2 cut(s) 1390, 2654
PspFI CCCAGC 2 cut(s) 1658, 2484
PspN4I GGNNCC 9 cut(s) 108, 732, 831, 1242, 2161, 2277, 3668, 3848, 4167
PspPPI RGGWCCY 4 cut(s) 721, 830, 2159, 4045
PsrI GAACNNNNNNTAC 2 cut(s) 2862, 2894
PstNI CAGNNNCTG 4 cut(s) 4805, 5621, 6623, 6797
PsuI RGATCY 8 cut(s) 730, 2814, 3235, 3524, 5273, 6619, 6759, 6793
PsyI GACNNNGTC 1 cut(s) 6362
PvuII CAGCTG 2 cut(s) 1247, 6128
RseI CAYNNNNRTG 8 cut(s) 2584, 2724, 3144, 3335, 3416, 4158, 5786, 6360
Rsr2I CGGWCCG 1 cut(s) 745
RsrII CGGWCCG 1 cut(s) 745
SacI GAGCTC 2 cut(s) 235, 2774
SapI GCTCTTC 2 cut(s) 153, 347
ScaI AGTACT 4 cut(s) 980, 1263, 3789, 4229
SchI GAGTC 8 cut(s) 80, 179, 2331, 3817, 4248, 5131, 5860, 6131
SduI GDGCHC 8 cut(s) 111, 165, 235, 1342, 1442, 2774, 3141, 3849
SexAI ACCWGGT 2 cut(s) 1716, 6171
SfcI CTRYAG 1 cut(s) 4252
Sfr274I CTCGAG 1 cut(s) 6139
SlaI CTCGAG 1 cut(s) 6139
SmiMI CAYNNNNRTG 8 cut(s) 2584, 2724, 3144, 3335, 3416, 4158, 5786, 6360
SnaBI TACGTA 1 cut(s) 1205
SpeI ACTAGT 2 cut(s) 2078, 5017
SphI GCATGC 1 cut(s) 2417
SspI AATATT 6 cut(s) 4178, 4374, 4968, 5108, 5196, 5414
SstI GAGCTC 2 cut(s) 235, 2774
StyI CCWWGG 8 cut(s) 165, 285, 328, 1953, 2408, 4944, 5232, 6156
TaiI ACGT 6 cut(s) 18, 1207, 1844, 2256, 3028, 3370
TaqII GACCGA 2 cut(s) 733, 900
TatI WGTACW 9 cut(s) 223, 978, 1261, 2938, 3109, 3787, 3797, 4227, 5437
TauI GCSGC 6 cut(s) 92, 241, 250, 1640, 2420, 5030
TseFI GTSAC 8 cut(s) 1390, 2358, 2549, 2654, 4924, 4972, 5905, 6667
Tsp45I GTSAC 8 cut(s) 1390, 2358, 2549, 2654, 4924, 4972, 5905, 6667
TspGWI ACGGA 2 cut(s) 737, 3235
Tth111I GACNNNGTC 1 cut(s) 6362
Vha464I CTTAAG 1 cut(s) 4280
VneI GTGCAC 2 cut(s) 1338, 3137
XbaI TCTAGA 1 cut(s) 3527
XceI RCATGY 6 cut(s) 1033, 1816, 2417, 3772, 4219, 5157
XcmI CCANNNNNNNNNTGG 4 cut(s) 2437, 3677, 6287, 6398
XhoI CTCGAG 1 cut(s) 6139
XmiI GTMKAC 3 cut(s) 1201, 1911, 6147
XmnI GAANNNNTTC 5 cut(s) 427, 4037, 4746, 5718, 6556
ZrmI AGTACT 4 cut(s) 980, 1263, 3789, 4229
Zsp2I ATGCAT 5 cut(s) 757, 2152, 3355, 4141, 4621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.