MD14G1001900.v1.1

HEAT repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002980
Physical Location & Seq
Forward (+)
314055 .. 334209
20155 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1001900.v1.1.491

Sequence Viewer

Length: 6654 bp
ATGGCGAAGAAGTATGTGACAACAGAGAATGCGCCCCTCTCTCAGTTCGGTGTATTGGTAGCGCAATTGGAGTCCATAGTCGCCTCAGCATCCCATAAGCCTCAGGAGCCACTTCTATGTTTCGATCTCCTCTCCGATCTCATATCCGCCATTAACGAAGAGCCCAAGGAATCCATTTTGTTGTGGCAAAGGAGATGCGAGGACGCATTATATTCCTTGCTTACTCTTGGTGCTCGGCGACCTGTCCGCCATCTGGCTTCAGTTGCTATGGCGAGGGTTATATCTAAGGGAGATGGCATTTCCATATACTCTCGAGCAAGCAGTCTCCAAGGCTTTCTTTCTGATGGAAGGAGGAATGAACCACAAAAAGTTGCTGGTGCTGCACAGTGCTTAGGAGAATTATATAGACATTTTGGGAGGCGAATCACATCGGGTTTACTTGAAACGACTATCATTGCCACGAAACTTTTCAAGTTTAATGAGGAATTTGTGAGACAAGAAGCTTTATATATGCTTCAAAATGCTTTGGAAGGCTCTGGTGGAAACGCAGCGGCTTCAGCATATACAGAGGCATTTCGTCTCATCATGCGGTTTGCAGTTGGTGATAAATCATTTCTTGTTAGAATTGCTGCTGCAAGATGTCTGAAGGCATTTGCCATTATTGGAGGACCTGGTCTAGGGGTGGGAGAGCTAGACAGTTCAGCTTCTTATTGTGTCAAGGCCCTTGAGGATCCTGTTTCTTCTGTCCGTGATGCATTTGCTGAAGCATTGGGTTCGTTGCTTGCTCTTGGAATGAATCCTCATGCACAGGTTCAACCAAGGGGGAAAGGACCTTTCCCTCCAGCAAAGAAACTGGAAGGTGGTTTGCATAGACATTTAGCTTTGCCTTTCACAAAAGTTGGAGCTCGGTCAAAGGACGTGCGAGTTGGCATAACCTTGTCTTGGGTATTCTTTTTACAGGCGATTCGTCTAAAATACATGCATCCAGATAGTGAGCTACAAAACTATGCAATTCAGGTTATGGAAATGCTTTGTTCCGATAATTCTGTTGATGCCCATGCACTGGCTTGTGTGCTCTATATCCTTCGTGTTGGTGTTACTGATCAAATGGCAGAGCCTACGCAAAGGAGTTTTTTGGGTTTTCTTGGGAATCAGCTTATGTCTCTAGATGCCAGTCCTTCCATGAAAATTGCCGCCCTACGTACTGCATCATATACCCTGAAAACGCTGGGAGAGGTTCCAGTTGATTTCAAGGAAGTTCTTGACAACACTGTTGTTGCAGCAGTATCTCATTCTTCACAACTTGTTCGAATTGAGGCTGCTTTGACATTACGTGCCCTGGCTGAGGTTGATCCCACCTGTGTTGGTGGTTTAATTTCTTATGGAGTGACCATGCTAAATGCTTTGAGAGAAAATGTGGCATATGAGAAGGGGAGCACTTTGCAATTAGAGCTTGATTCTTTGCATGGACAGGCTACTGTCTTGGCAGCTCTGGTGTCCATTTCCCCAAAATTACCTCTCGGTTTTCCTGCCAGATTGCCCAGGTCAATCCTTGAAGTTTCAAAGAAGATGTTGAATGAATCTAGTCGGAACCCTATGGCAGCTACAATTGAAAAAGAAGCTGGGTGGTTACTTTTGTCACCCCTGTTAGCTTCTATGCCAAATGAGGAACTTGAAGACCAGGTTTTTGATATTCTTTCCTTGTGGGCTTCCCTCTTTACTGGAAATCCAGATGATGAAACCAACCAAACTGGAGATTTGACGAGTAGGATACGCATGTGGTCTGCTGCAGTTGATGCACTTACTGCATTTTTAAGATGCTTTCTTTCTCCTAATGATGGGAATAACGGGATTTTCCTTCAACCTGTACTTGTATACCTGAGTAGGGCTTTATCGTTTATATCGCTGATAGCAGCCAAGCAACTGCCAAATGTGAAGCCTGCTTTGGATATATTTATCATCCGGACATTAATAGCTTACCAGTCTCTTCCTGATCCAACAGCATATAAGAATGACCACCCCATGGTCCTTCAAATTTGCACAAGCCCATTCATAGAGGCTTCTGGATGTGAAGAAAGTACATGCTTGAGGTTTCTGTTAGACAAAAGAGATGCATGGTTGGGTCCCTGGATTCCTGGAAGAGATTGGTTTGAAGATGAACTTCGTGCTTTTCAAGGTGGCAAAAACGGCCTTATTCCTTGTGTCTGGGAGAATGAAGTTTGTAGCTTTCCTCAGCCGGAACCAGTAAACAAGACATTGGTGAACCAGATGCTCCTCTGCTTTGGCGTCTTGTTTGCCTCTCAGGATAGTGGGGGGATGCTGTCACTTCTTGGAATGATTGAGCAGTGCCTGAAAGCTGGAAAAAAGCAACCATGGCATGCAGCGAGCATAACCAATATATGTGTGGGGTTACTTTCTGGATTTAAGGCTTTGCTTTCCTTGCGTCCCCAGCCATTATCACTTGAGATACTAAATTCCGCACAGGCGATATTTCAGAGCATTCTGGCAGAGGGAGACATCTGTCCATCACAACGTAGGGCTTCATCAGAATGTCTTGGTCTTTTAGCTCGTCTTGGAAACGATATCTTTACAGCAAGAATGACTAGATCTCTGCTTGGTGACCTAACTGGGGCAACAGATTCAAACTATGCTGGATCAATTGCTTTTGCTCTTGGCTGCATTCATCGCAGTGCAGGAGGCATGGCATTGTCAACTTTAGTGCCTTCAACTGTGAGCTCAATCTCGTTGCTTTCTAAAAGTTCCATAGCTGGTTTACAGATATGGTCTTTGCATGGGCTTCTGTTGACCATTGAAGCTGCTGGCTTGTCCTTTGTATCTCAAGTCCAGGCAACACTAGGTCTTGCTCTGGAAATTCTTTTGTCCGAAGAGAATGGATGGGTTGCACTTCAGCAAGGTGTTGGTCGTCTTATAAATGCTATTGTTGCTGTTCTTGGTCCTGAACTTGCCCCTGGCAGTATTTTCTTTTCACGTTGCCAGTCTGTTGTTTCAGAGATAAGCTCTGGGCAAGAAACAGCAACGATGCTCGAGAGTGTACGCTTTACCCAGCAACTTGTTCTTTTTGCACCACAAGCTGTTTCCGTGCACACCCATGTGCAAACTCTTCTACCTACTTTGGCCTCAAGACAGCCAGCATTAAGGCATCTAGCTGTTTCCACTCTACGACATCTGATTGAAAAAGATCCAGTCTCCATTGTTGTTGAGCAAATAGAGGAGAGGTTATTTCACATGCTGGATGAAGAAACAGATTCTGAGATAGGGGATCTGGTGCGCACCACAATCATGCGATTGCTCTATGCATCATGCCCTTCCTGTCCATCTCATTGGATATCAATTTGTCGTAATGTGATTCTTGCCACATCAACGCGAAGAAATGCTGATAGTAGTAGCAGCTTAGAAAATGAATCTTCAAAGGGTACAGAGGGTGACCTAAGTGTAAATTTTGGAGAAGACGATGAGAACATGGTTTCTAGCACCACAGGTGTGCTCCATGGTATTCTGAACAGAGATAAACACCTCAGATACAGAACCAGAGTTTTTGCTGCTGAGTGTTTGAGTTACCTACCTAGAGCTGTTGGAAAGAATCCGGCTCATTTTGATCTCTGTACTGCTAGGAGCCAACCTACTAATAGACAGGCCTCTAGTGATTGGCTCGTCCTCCATATACAAGAACTTATAGCTCTTGCATACCAGATAAGCACGATCCAGTTGGAAAACATGCAGCCAATTGGTGTGGGGCTTCTGAGTACCATTACAGATAAGTTTGAAAAGACACCTGACCCGGAGCTTCCAGGGCACCTTCTACTAGAACAGTATCAGGCCCAACTCGTATCTGCTGTCCGCACTGCCTTAGACTCGTCCTCTGGCCCTATTCTATTGGAAGCAGGCTTCCAGCTCGCTACGAAGATATTGACTAGTGGAATAATTAAGGGCGATCAAATTGCTGTTAAACGCGTATATTCATTAATTTCACGACCATTGAATGAATTTAAGGACCTGTATTATCCTTCATTTGCAGAATGGGTCTCATGCAAGATCAAGATAAGACTTCTTGCTGCTCATGCCTCTCTCAAATGTTATACATATGCGTTCTTGAGGAGACACCATAGTGGGGTTCCCGATGAATATCTAGCATTGTTACCATTATTTTCAAAGAGTTCAAGTATGCTTGGGAAGTACTGGATTAGTGTTTTGAAGGATTACAGTTATGTATTCTTGTGCTTGCATCTCAAGACAAAGTGGAATCCATTTCTTGATGGAATTCAGTCACCTCTGGTATCATCAAAGTTGCAACCTTGCTTAGAAGAATCGTGGCCAGTAATATTGCAGGCAATTGCATTGGATGCAGTTCCTGTGAATATTGAAGAGAACGAGTATTCAAACTCCACTACTCAAAATATATCAAGCAATAACTTATTGTCTGGACATGGCATGGTTAAATTGGAATCCGAAGAATATCAATTTCTTTGGGGATTTGCTCTACTTGTCTTATTTCAGGGCCAATATTCTACCCTTGGTGAACTGAAAAGTCCACTTTATTTCATTAAAGCTAGTAACGGTGGAGATTCAGCTAGTGAAGGGTTATCTTTCCCAGGAATTAAGTTATATGAAATTGCATTGCCGGTTTTCCAGTTTCTGTCGACCAAAAGGTTTGCCAGTGCGGGATTTCTCACTCTGGACATATGCAGAGAACTGCTACAGGTTTTCTCATATTCTATGTGCATGGATAATTCCTGGGATAGTCTTTCAGTGTCTGTTATATCACAGATTGTGAAGAACTGCCCTGAATCTTTTTATGAAGTGGACAACTTTGCTTATTTGGCAATGGAACTTTGTTTGGCTTATCTTTACAAAGTATTTCAAGGTAGTAAGGCAATTTCTCTGGACAAACCGTCAGAGGATTTGATATCCACATTATTTATCACTGCAGAAACACTTGTAAACTGTTTTCAACCAAAGACACAACTAGTCTCTGCGGCATTGGCGTTTCTTTTAATTGGTTACAAGGGAATCAGAGAAGCCTCGACTGAGTTTTATTTCTCCAAGGTGGACAAATTTTTCAAGTGCACAAGTCTATTATTGAAAAGATTTATTGATGATAAATCCGGTGTTGGTGAAGATGGTCTTCTTCATATGCGAAAGTTATTGGGAACTTGTCTGAATGTGATGACTGATTTGACCGAGGATTGCATTAAGTCCATTCATCTGCAGGAAAATAAGAGATCCGACTTGCACATACTACTGCAAAGAAAGCTTGCTTTCTCTATTGAACAAACTATATCATTTGCCAAGTTGGGTTATGAAATGGATTATCTTGATGAGAATAGAGATGGTGACTCCGTCTATTATCCTATGTTTAAATACTGCACAAAATGTGTTCAAACTGTACTTAGCGATTCAAATGTTCAGGTTCAATCAATTGGGTTACAATTGCTAAAAAGTTTGGTACAGAAAACCCCAAATGCGGAAGGCAACAATTTTTTAATGCTTTTTGTTGGGGAGCTTACAGCAGATTTTTTTGTGATAATCCAGAATGCTTTAAAGAAACCTGTAACTGAAAAATCAGCAACTGTTGCTGGTGACTGCTTAAGACTCTTAGTAGTGCTGCAAACACTTTCAAAACCTAGTGAATGCCAGAGAGGTTTTATGAATTTACTCCTGGAAGCCGTAGTCGTGGTTTTCAAGGCTTCAGAGGAAGGTTCTTCACTGGAAGTCAATACATTAAGAAGCACTGCTGTAAGGCTTGTTTCTCATCTTGCTCAGCTTCCTTCCTCAGCAGTTCACTTCAAGGATGTATTGCTGTCTATGCCTGTAACACATCGACAGCAACTACAGGGGTTCATCCGTGCGTCTGTGACTCAAGAGCATAATGCAACAGAGCTGAAGCCCACAACTCCGTCCTTAGAGATAAAATTACCGGTTCCAAAAGAGGCAAGCAAAGAGAAACCTCTGCCATCAGCCACCACAGCGCATTCTGTTTCTGATGAACAAGAAATCGAAGAGGAAGAAGAGGATGAAGATGATTGGGATGCTTTCCAGTCTTTTCCTGCCGCTTTAAATGCAGCTGAAAGTGAACCGAAAGTAGAAAGCAGAGTGGAAGAACCTGACTTGGAAGTGAATACGCAGAGTGATTGCAGTCATGGAGAATCTATTCTGCAACCTCCCAACAACGTGGATGCAGTCAACGATACAGACCATCACGAGGCTGGTGAAAGGGAGGTGATATTAGATGCCCCTGATGGCCTAAAATCCCCACAGGGTAACCCACCAACCAACAATACTGAGGCAGAAGAACCCCGTGATCTCCAAACTAATAGCGGTATTATCGGGCCAAACGATGATCAGCCTTTGATGAGAGACGAGGAAGTGGTTTCCAGACAAGAAGGAAGGGCAGCAGGGTTGAACCAAGTGACGACTGAGCATTTGGCATCTGAACTTGATCATCCTAGTGAAGATGCTGAAGCATCAGTTGAGATGAATTTAGGGCATCATGTGCAGGAGAAAAAAGAGAAGCGTGATGATAAAGCAGGGCAGGTATCATCAGATCCTCTGCCACTTGATGAGGAAGTACGTGATAAAAGAGAAGACAAGGAAGAAGCAGCTTCCGAAAAGTCTCGCCTCGAGCAGTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

2218

Amino Acids

243.55

Weight (kDa)

5.3

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Laa1_Sip1_HTR5 PF20210 1223 - 1408 5.7e-44 Laa1/Sip1/HEATR5 HEAT repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2930
Acc16I TGCGCA 1 cut(s) 3290
Acc36I ACCTGC 1 cut(s) 6541
AccB1I GGYRCC 1 cut(s) 3813
AccB7I CCANNNNNTGG 2 cut(s) 1063, 2023
AccI GTMKAC 2 cut(s) 1875, 4658
AccII CGCG 2 cut(s) 3385, 3972
AccIII TCCGGA 1 cut(s) 1962
AcoI YGGCCR 1 cut(s) 4331
AcuI CTGAAG 8 cut(s) 243, 540, 665, 783, 2891, 5691, 5921, 6498
AcyI GRCGYC 1 cut(s) 2286
AflII CTTAAG 1 cut(s) 5605
AflIII ACRYGT 1 cut(s) 3970
AgeI ACCGGT 1 cut(s) 5935
AhdI GACNNNNNGTC 1 cut(s) 4909
AhlI ACTAGT 2 cut(s) 3932, 4984
AjiI CACGTC 1 cut(s) 919
AjuI GAANNNNNNNTTGG 2 cut(s) 1928, 1960
AleI CACNNNNGTG 3 cut(s) 3110, 3500, 4125
Alw21I GWGCWC 8 cut(s) 235, 907, 1077, 1439, 2738, 3105, 3507, 5087
Alw44I GTGCAC 2 cut(s) 3101, 5083
AlwNI CAGNNNCTG 6 cut(s) 2350, 3269, 4370, 4654, 4772, 5588
Ama87I CYCGRG 3 cut(s) 312, 3044, 6638
Aor13HI TCCGGA 1 cut(s) 1962
ApaLI GTGCAC 2 cut(s) 3101, 5083
ArsI GACNNNNNNTTYG 4 cut(s) 1670, 1702, 6424, 6456
AseI ATTAAT 2 cut(s) 1970, 3983
AsiGI ACCGGT 1 cut(s) 5935
Asp700I GAANNNNTTC 2 cut(s) 467, 6168
AspLEI GCGC 4 cut(s) 34, 64, 3291, 5989
AsuC2I CCSGG 1 cut(s) 3800
AsuII TTCGAA 1 cut(s) 1309
AvaI CYCGRG 3 cut(s) 312, 3044, 6638
AvaII GGWCC 6 cut(s) 668, 830, 2026, 2123, 2954, 4012
AxyI CCTNAGG 1 cut(s) 102
BaeGI GKGCMC 4 cut(s) 1339, 3105, 3816, 5087
BaeI ACNNNNGTAYC 2 cut(s) 1763, 1796
BalI TGGCCA 1 cut(s) 4333
BamHI GGATCC 1 cut(s) 730
BanI GGYRCC 1 cut(s) 3813
BanII GRGCYC 3 cut(s) 165, 907, 2738
BauI CACGAG 1 cut(s) 6218
BbsI GAAGAC 4 cut(s) 1683, 3474, 5135, 6609
Bbv12I GWGCWC 8 cut(s) 235, 907, 1077, 1439, 2738, 3105, 3507, 5087
BbvCI CCTCAGC 4 cut(s) 85, 1344, 2232, 5791
BceAI ACGGC 2 cut(s) 2203, 5669
BcgI CGANNNNNNTGC 4 cut(s) 756, 790, 3941, 3975
BciVI GTATCC 1 cut(s) 1764
BclI TGATCA 3 cut(s) 1102, 6358, 6457
BcnI CCSGG 1 cut(s) 3800
BcuI ACTAGT 2 cut(s) 3932, 4984
BfmI CTRYAG 5 cut(s) 1788, 4715, 4944, 5225, 5849
BfrI CTTAAG 1 cut(s) 5605
BfuAI ACCTGC 1 cut(s) 6541
BfuI GTATCC 1 cut(s) 1764
BglII AGATCT 1 cut(s) 2606
BlpI GCTNAGC 1 cut(s) 5778
BmcAI AGTACT 1 cut(s) 4196
Bme18I GGWCC 6 cut(s) 668, 830, 2026, 2123, 2954, 4012
BmeRI GACNNNNNGTC 1 cut(s) 4909
BmeT110I CYCGRG 3 cut(s) 312, 3044, 6638
BmgBI CACGTC 1 cut(s) 919
BmrI ACTGGG 1 cut(s) 2637
BmuI ACTGGG 1 cut(s) 2637
BoxI GACNNNNGTC 1 cut(s) 2520
BpiI GAAGAC 4 cut(s) 1683, 3474, 5135, 6609
BplI GAGNNNNNCTC 2 cut(s) 3002, 3034
BpmI CTGGAG 2 cut(s) 825, 1773
Bpu10I CCTNAGC 5 cut(s) 85, 391, 1344, 2232, 5791
Bpu1102I GCTNAGC 1 cut(s) 5778
Bpu14I TTCGAA 1 cut(s) 1309
BpuEI CTTGAG 8 cut(s) 746, 2107, 2483, 2823, 3124, 4132, 4232, 5862
BpuMI CCSGG 1 cut(s) 3800
BsaAI YACGTR 3 cut(s) 1202, 1334, 6590
BsaBI GATNNNNATC 1 cut(s) 4143
BsaHI GRCGYC 1 cut(s) 2286
BsaI GGTCTC 1 cut(s) 4048
BsaWI WCCGGW 3 cut(s) 1962, 5123, 5935
Bse118I RCCGGY 2 cut(s) 4639, 5935
Bse21I CCTNAGG 1 cut(s) 102
Bse3DI GCAATG 3 cut(s) 453, 4634, 4848
Bse8I GATNNNNATC 1 cut(s) 4143
BseAI TCCGGA 1 cut(s) 1962
BseJI GATNNNNATC 1 cut(s) 4143
BseMI GCAATG 3 cut(s) 453, 4634, 4848
BseRI GAGGAG 4 cut(s) 119, 2264, 3245, 4129
BseSI GKGCMC 4 cut(s) 1339, 3105, 3816, 5087
BseYI CCCAGC 4 cut(s) 1228, 1622, 2448, 3063
BsgI GTGCAG 4 cut(s) 366, 2712, 5368, 6533
Bsh1236I CGCG 2 cut(s) 3385, 3972
BshNI GGYRCC 1 cut(s) 3813
BshTI ACCGGT 1 cut(s) 5935
BsiHKAI GWGCWC 8 cut(s) 235, 907, 1077, 1439, 2738, 3105, 3507, 5087
BsiHKCI CYCGRG 3 cut(s) 312, 3044, 6638
BsiSI CCGG 7 cut(s) 1963, 2237, 3605, 3800, 4640, 5124, 5936
BslFI GGGAC 2 cut(s) 2109, 2430
BsmBI CGTCTC 2 cut(s) 584, 6369
BsmFI GGGAC 2 cut(s) 2109, 2430
BsmI GAATGC 6 cut(s) 34, 2499, 2679, 5557, 5654, 5989
Bso31I GGTCTC 1 cut(s) 4048
BsoBI CYCGRG 3 cut(s) 312, 3044, 6638
Bsp119I TTCGAA 1 cut(s) 1309
Bsp13I TCCGGA 1 cut(s) 1962
Bsp1720I GCTNAGC 1 cut(s) 5778
Bsp19I CCATGG 3 cut(s) 2022, 2372, 3508
BspEI TCCGGA 1 cut(s) 1962
BspFNI CGCG 2 cut(s) 3385, 3972
BspMAI CTGCAG 3 cut(s) 1792, 4948, 5229
BspMI ACCTGC 1 cut(s) 6541
BspQI GCTCTTC 1 cut(s) 153
BspT104I TTCGAA 1 cut(s) 1309
BspT107I GGYRCC 1 cut(s) 3813
BspTI CTTAAG 1 cut(s) 5605
BspTNI GGTCTC 1 cut(s) 4048
BsrDI GCAATG 3 cut(s) 453, 4634, 4848
BsrFI RCCGGY 2 cut(s) 4639, 5935
BssAI RCCGGY 2 cut(s) 4639, 5935
BssNAI GTATAC 1 cut(s) 1876
BssNI GRCGYC 1 cut(s) 2286
BssSI CACGAG 1 cut(s) 6218
BssT1I CCWWGG 8 cut(s) 165, 328, 818, 2022, 2372, 3508, 4531, 5061
Bst1107I GTATAC 1 cut(s) 1876
Bst2BI CACGAG 1 cut(s) 6218
Bst6I CTCTTC 8 cut(s) 153, 1992, 2134, 2880, 3126, 4377, 6012, 6021
BstACI GRCGYC 1 cut(s) 2286
BstAFI CTTAAG 1 cut(s) 5605
BstAPI GCANNNNNTGC 5 cut(s) 1796, 1805, 4361, 5591, 6511
BstBAI YACGTR 3 cut(s) 1202, 1334, 6590
BstBI TTCGAA 1 cut(s) 1309
BstDSI CCRYGG 3 cut(s) 2022, 2372, 3508
BstEII GGTNACC 3 cut(s) 2618, 3443, 6278
BstENI CCTNNNNNAGG 2 cut(s) 675, 1343
BstFNI CGCG 2 cut(s) 3385, 3972
BstHHI GCGC 4 cut(s) 34, 64, 3291, 5989
BstNSI RCATGY 6 cut(s) 982, 1780, 2085, 2381, 3250, 3739
BstPAI GACNNNNGTC 1 cut(s) 2520
BstPI GGTNACC 3 cut(s) 2618, 3443, 6278
BstSFI CTRYAG 5 cut(s) 1788, 4715, 4944, 5225, 5849
BstSLI GKGCMC 4 cut(s) 1339, 3105, 3816, 5087
BstSNI TACGTA 1 cut(s) 1202
BstUI CGCG 2 cut(s) 3385, 3972
BstV2I GAAGAC 4 cut(s) 1683, 3474, 5135, 6609
BstX2I RGATCY 6 cut(s) 730, 2606, 3199, 3280, 5240, 6562
BstXI CCANNNNNNTGG 2 cut(s) 3342, 6190
BstYI RGATCY 6 cut(s) 730, 2606, 3199, 3280, 5240, 6562
BstZ17I GTATAC 1 cut(s) 1876
Bsu36I CCTNAGG 1 cut(s) 102
BsuI GTATCC 1 cut(s) 1764
BtgI CCRYGG 3 cut(s) 2022, 2372, 3508
BtgZI GCGATG 1 cut(s) 2669
BtrI CACGTC 1 cut(s) 919
BtsI GCAGTG 5 cut(s) 2351, 2695, 3861, 4941, 5748
BveI ACCTGC 1 cut(s) 6541
CaiI CAGNNNCTG 6 cut(s) 2350, 3269, 4370, 4654, 4772, 5588
CfoI GCGC 4 cut(s) 34, 64, 3291, 5989
Cfr10I RCCGGY 2 cut(s) 4639, 5935
CseI GACGC 4 cut(s) 212, 2275, 2432, 5856
CsiI ACCWGGT 2 cut(s) 670, 1680
CspAI ACCGGT 1 cut(s) 5935
CspCI CAANNNNNGTGG 4 cut(s) 1345, 1380, 3732, 3767
DraI TTTAAA 3 cut(s) 5377, 5559, 6075
DriI GACNNNNNGTC 1 cut(s) 4909
EaeI YGGCCR 1 cut(s) 4331
Eam1104I CTCTTC 8 cut(s) 153, 1992, 2134, 2880, 3126, 4377, 6012, 6021
Eam1105I GACNNNNNGTC 1 cut(s) 4909
EarI CTCTTC 8 cut(s) 153, 1992, 2134, 2880, 3126, 4377, 6012, 6021
EciI GGCGGA 2 cut(s) 136, 236
Ecl136II GAGCTC 2 cut(s) 905, 2736
Eco105I TACGTA 1 cut(s) 1202
Eco130I CCWWGG 8 cut(s) 165, 328, 818, 2022, 2372, 3508, 4531, 5061
Eco147I AGGCCT 1 cut(s) 3656
Eco24I GRGCYC 3 cut(s) 165, 907, 2738
Eco31I GGTCTC 1 cut(s) 4048
Eco32I GATATC 3 cut(s) 2584, 3348, 4926
Eco47I GGWCC 6 cut(s) 668, 830, 2026, 2123, 2954, 4012
Eco53kI GAGCTC 2 cut(s) 905, 2736
Eco57I CTGAAG 8 cut(s) 243, 540, 665, 783, 2891, 5691, 5921, 6498
Eco81I CCTNAGG 1 cut(s) 102
Eco88I CYCGRG 3 cut(s) 312, 3044, 6638
Eco91I GGTNACC 3 cut(s) 2618, 3443, 6278
EcoICRI GAGCTC 2 cut(s) 905, 2736
EcoNI CCTNNNNNAGG 2 cut(s) 675, 1343
EcoO109I RGGNCCY 5 cut(s) 668, 721, 830, 2123, 4012
EcoO65I GGTNACC 3 cut(s) 2618, 3443, 6278
EcoRI GAATTC 1 cut(s) 4278
EcoRV GATATC 3 cut(s) 2584, 3348, 4926
EcoT14I CCWWGG 8 cut(s) 165, 328, 818, 2022, 2372, 3508, 4531, 5061
EcoT22I ATGCAT 4 cut(s) 757, 984, 2116, 3319
EcoT38I GRGCYC 3 cut(s) 165, 907, 2738
ErhI CCWWGG 8 cut(s) 165, 328, 818, 2022, 2372, 3508, 4531, 5061
Esp3I CGTCTC 2 cut(s) 584, 6369
FalI AAGNNNNNCTT 6 cut(s) 321, 353, 2145, 2177, 5127, 5159
FaqI GGGAC 2 cut(s) 2109, 2430
FauI CCCGC 1 cut(s) 4672
FauNDI CATATG 4 cut(s) 1423, 4102, 4700, 5151
FbaI TGATCA 3 cut(s) 1102, 6358, 6457
FblI GTMKAC 2 cut(s) 1875, 4658
FriOI GRGCYC 3 cut(s) 165, 907, 2738
FspAI RTGCGCAY 1 cut(s) 3290
FspI TGCGCA 1 cut(s) 3290
GlaI GCGC 4 cut(s) 33, 63, 3290, 5988
GsaI CCCAGC 4 cut(s) 1232, 1626, 2452, 3067
GsuI CTGGAG 2 cut(s) 825, 1773
HapII CCGG 7 cut(s) 1963, 2237, 3605, 3800, 4640, 5124, 5936
HgaI GACGC 4 cut(s) 212, 2275, 2432, 5856
HhaI GCGC 4 cut(s) 34, 64, 3291, 5989
Hin1I GRCGYC 1 cut(s) 2286
Hin6I GCGC 4 cut(s) 32, 62, 3289, 5987
HinP1I GCGC 4 cut(s) 32, 62, 3289, 5987
HincII GTYRAC 4 cut(s) 2712, 2805, 4659, 6202
HindII GTYRAC 4 cut(s) 2712, 2805, 4659, 6202
HindIII AAGCTT 2 cut(s) 501, 5270
HpaII CCGG 7 cut(s) 1963, 2237, 3605, 3800, 4640, 5124, 5936
HpyCH4IV ACGT 7 cut(s) 918, 1201, 1333, 2533, 2989, 6189, 6589
HpySE526I ACGT 7 cut(s) 918, 1201, 1333, 2533, 2989, 6189, 6589
Hsp92I GRCGYC 1 cut(s) 2286
HspAI GCGC 4 cut(s) 32, 62, 3289, 5987
KflI GGGWCCC 1 cut(s) 2123
Kpn2I TCCGGA 1 cut(s) 1962
Ksp22I TGATCA 3 cut(s) 1102, 6358, 6457
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 8 cut(s) 106, 902, 1434, 2277, 3510, 3633, 3802, 5518
MabI ACCWGGT 2 cut(s) 670, 1680
MaeII ACGT 7 cut(s) 918, 1201, 1333, 2533, 2989, 6189, 6589
MfeI CAATTG 7 cut(s) 65, 1608, 2658, 3744, 4350, 5436, 5447
MflI RGATCY 6 cut(s) 730, 2606, 3199, 3280, 5240, 6562
MlsI TGGCCA 1 cut(s) 4333
MluI ACGCGT 1 cut(s) 3970
MluNI TGGCCA 1 cut(s) 4333
MlyI GAGTC 5 cut(s) 80, 3866, 5348, 5604, 5869
MmeI TCCRAC 6 cut(s) 880, 1568, 2021, 3574, 3708, 5268
Mox20I TGGCCA 1 cut(s) 4333
Mph1103I ATGCAT 4 cut(s) 757, 984, 2116, 3319
MroI TCCGGA 1 cut(s) 1962
MroXI GAANNNNTTC 2 cut(s) 467, 6168
MscI TGGCCA 1 cut(s) 4333
MslI CAYNNNNRTG 9 cut(s) 115, 2548, 2688, 3108, 3110, 3299, 3500, 4125, 6465
Msp20I TGGCCA 1 cut(s) 4333
MspA1I CMGCKG 2 cut(s) 551, 6083
MspCI CTTAAG 1 cut(s) 5605
MspI CCGG 7 cut(s) 1963, 2237, 3605, 3800, 4640, 5124, 5936
MunI CAATTG 7 cut(s) 65, 1608, 2658, 3744, 4350, 5436, 5447
Mva1269I GAATGC 6 cut(s) 34, 2499, 2679, 5557, 5654, 5989
MvnI CGCG 2 cut(s) 3385, 3972
NciI CCSGG 1 cut(s) 3800
NcoI CCATGG 3 cut(s) 2022, 2372, 3508
NdeI CATATG 4 cut(s) 1423, 4102, 4700, 5151
NmeAIII GCCGAG 1 cut(s) 214
NsbI TGCGCA 1 cut(s) 3290
NsiI ATGCAT 4 cut(s) 757, 984, 2116, 3319
NspI RCATGY 6 cut(s) 982, 1780, 2085, 2381, 3250, 3739
NspV TTCGAA 1 cut(s) 1309
OliI CACNNNNGTG 3 cut(s) 3110, 3500, 4125
PaeI GCATGC 1 cut(s) 2381
PaeR7I CTCGAG 3 cut(s) 312, 3044, 6638
PceI AGGCCT 1 cut(s) 3656
PciSI GCTCTTC 1 cut(s) 153
PcsI WCGNNNNNNNCGW 1 cut(s) 6351
PctI GAATGC 6 cut(s) 34, 2499, 2679, 5557, 5654, 5989
PdmI GAANNNNTTC 2 cut(s) 467, 6168
PflFI GACNNNGTC 2 cut(s) 672, 5357
PflMI CCANNNNNTGG 2 cut(s) 1063, 2023
PfoI TCCNGGA 2 cut(s) 2134, 5676
PinAI ACCGGT 1 cut(s) 5935
PleI GAGTC 5 cut(s) 79, 3866, 5348, 5604, 5869
PpsI GAGTC 5 cut(s) 79, 3866, 5348, 5604, 5869
Ppu21I YACGTR 3 cut(s) 1202, 1334, 6590
PpuMI RGGWCCY 4 cut(s) 668, 830, 2123, 4012
PshAI GACNNNNGTC 1 cut(s) 2520
PshBI ATTAAT 2 cut(s) 1970, 3983
PsiI TTATAA 1 cut(s) 2930
Psp124BI GAGCTC 2 cut(s) 907, 2738
Psp5II RGGWCCY 4 cut(s) 668, 830, 2123, 4012
PspEI GGTNACC 3 cut(s) 2618, 3443, 6278
PspFI CCCAGC 4 cut(s) 1228, 1622, 2448, 3063
PspPPI RGGWCCY 4 cut(s) 668, 830, 2123, 4012
PspXI VCTCGAGB 1 cut(s) 6638
PstI CTGCAG 3 cut(s) 1792, 4948, 5229
PstNI CAGNNNCTG 6 cut(s) 2350, 3269, 4370, 4654, 4772, 5588
PsuI RGATCY 6 cut(s) 730, 2606, 3199, 3280, 5240, 6562
PsyI GACNNNGTC 2 cut(s) 672, 5357
PvuII CAGCTG 1 cut(s) 6083
RseI CAYNNNNRTG 9 cut(s) 115, 2548, 2688, 3108, 3110, 3299, 3500, 4125, 6465
SacI GAGCTC 2 cut(s) 907, 2738
SalI GTCGAC 1 cut(s) 4657
SapI GCTCTTC 1 cut(s) 153
ScaI AGTACT 1 cut(s) 4196
SchI GAGTC 5 cut(s) 80, 3866, 5348, 5604, 5869
SexAI ACCWGGT 2 cut(s) 670, 1680
SfcI CTRYAG 5 cut(s) 1788, 4715, 4944, 5225, 5849
Sfr274I CTCGAG 3 cut(s) 312, 3044, 6638
SfuI TTCGAA 1 cut(s) 1309
SinI GGWCC 6 cut(s) 668, 830, 2026, 2123, 2954, 4012
SlaI CTCGAG 3 cut(s) 312, 3044, 6638
SmiMI CAYNNNNRTG 9 cut(s) 115, 2548, 2688, 3108, 3110, 3299, 3500, 4125, 6465
SnaBI TACGTA 1 cut(s) 1202
SpeI ACTAGT 2 cut(s) 3932, 4984
SphI GCATGC 1 cut(s) 2381
SseBI AGGCCT 1 cut(s) 3656
SspI AATATT 3 cut(s) 4341, 4378, 4523
SstI GAGCTC 2 cut(s) 907, 2738
StuI AGGCCT 1 cut(s) 3656
StyI CCWWGG 8 cut(s) 165, 328, 818, 2022, 2372, 3508, 4531, 5061
TaiI ACGT 7 cut(s) 921, 1204, 1336, 2536, 2992, 6192, 6592
TaqI TCGA 9 cut(s) 123, 313, 1309, 3045, 4658, 5042, 5839, 6015, 6639
TaqII GACCGA 2 cut(s) 897, 5213
TatI WGTACW 5 cut(s) 1867, 2078, 3623, 4194, 5404
TauI GCSGC 4 cut(s) 554, 1196, 4997, 6071
TspGWI ACGGA 5 cut(s) 737, 3088, 5347, 5852, 5904
Tth111I GACNNNGTC 2 cut(s) 672, 5357
Van91I CCANNNNNTGG 2 cut(s) 1063, 2023
Vha464I CTTAAG 1 cut(s) 5605
VneI GTGCAC 2 cut(s) 3101, 5083
VpaK11BI GGWCC 6 cut(s) 668, 830, 2026, 2123, 2954, 4012
VspI ATTAAT 2 cut(s) 1970, 3983
XagI CCTNNNNNAGG 2 cut(s) 675, 1343
XbaI TCTAGA 1 cut(s) 1165
XceI RCATGY 6 cut(s) 982, 1780, 2085, 2381, 3250, 3739
XcmI CCANNNNNNNNNTGG 2 cut(s) 2401, 6221
XhoI CTCGAG 3 cut(s) 312, 3044, 6638
XmiI GTMKAC 2 cut(s) 1875, 4658
XmnI GAANNNNTTC 2 cut(s) 467, 6168
ZrmI AGTACT 1 cut(s) 4196
Zsp2I ATGCAT 4 cut(s) 757, 984, 2116, 3319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.