Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003221

Physical Location & Seq

Genomic Coordinates Reverse (-)

448756 .. 450177

Gene Structure 1422 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1002800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 753 bp

ATGTCATCCGAATCACAGACTCTAGATTCCTTTGCTGAATACGACTCATTTGTTGAGACACCTATAACAATGAGTTGTCAGTGTAGCCGAAACTCAATTTATGATTGTAGGAGTGTAAACCGGAAGCTTTGGAGTCATGAGAATGGAGGAGTTGATGTGTCTCTCAAGGATGGTTTCTTCTCGGAGATTGCTCAGGGTAGTGCCTACTCAAATGGATATCCAGGTAACCCTGGGATTAATGAATTCGTGAAATGTGAAGGAGATAGTAATGAGGAATTTACGGGTTCCAGCAAACAAATCCTAAAAATGGAGTATCAAGAAGCACCACAACTAGTCCTTGGAAAAGAGTGCCAATCTTTGGGATGGCATGACTCTCAGAGGTCTCGCACTCCACTCAATGTGGACAGCATCATCTTCAATACTCCGAGAAGGCTTGTTCACTCTCTTCAGGAACCAAATAATGCCGACTCAAAGTTCTCCAAGAAACCAGCGAGAAGATCTAGAAGTTTATCCATGAACGAGTTTGATTGGAGCCCAAATGCCAGATACGATCAAAATGGTTATTCATATGGTGCAAATTATGATGACGGAAAGCATGGTAGCTTCTATGGCAGCGGTGAGAAGTTTGAAGGTGGTCCTGAATCAGTGTCATCAACAGATGGCATTCCAACAGATGCTGATTTGCAGGTGTCTCATGAGGTGGTTCCAGAAAACAAAATATCTCATGAGGCGGTTCCAGAAAATAAAACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0006950 GO:0006952 GO:0006955 GO:0008092 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0012505 GO:0031967 GO:0031975 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0061760 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

251

Amino Acids

27.8

Weight (kDa)

4.9

Isoelectric Point (pI)

61.03

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54385 AT1G54385 AT1G54385 AT3G03970 AT3G03970 AT3G03970 AT3G03970 AT3G03970 AT3G03970
fragaria_vesca	FvH4_1g28060 FvH4_1g28060 FvH4_1g28060 FvH4_5g28880 FvH4_5g28880 FvH4_5g28880 FvH4_5g28880 FvH4_5g28880
malus_domestica	MD12G1002800.v1.1 MD12G1002900.v1.1 MD12G1003000.v1.1 MD14G1002300.v1.1
prunus_persica	Prupe.7G003500_v2.0.a1 Prupe.7G003500_v2.0.a1
pyrus_communis	pycom03g08170 pycom12g00260
rosa_chinensis	RchiOBHm_Chr3g0497191
rosa_laevigata	RLG00000022472
rosa_multiflora	Rmu_co8084920.1_g000001 Rmu_sc0002893.1_g000012 Rmu_sc0007568.1_g000001
rosa_roxburghii	Rroxscaffold_6G00388290
rosa_rugosa	Rorug03G0291700
rosa_samantha	Rh3AG335000 Rh3BG372700 Rh3CG369100 Rh3DG370000
rosa_wichuraiana	Rw3G029450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	676
Acc36I	ACCTGC	1 cut(s)	676
AccB7I	CCANNNNNTGG	1 cut(s)	358
AciI	CCGC	2 cut(s)	615, 731
AcsI	RAATTY	2 cut(s)	242, 275
AcuI	CTGAAG	1 cut(s)	431
AfiI	CCNNNNNNNGG	2 cut(s)	307, 358
AgsI	TTSAA	2 cut(s)	418, 629
AhlI	ACTAGT	1 cut(s)	331
AjnI	CCWGG	2 cut(s)	220, 229
AluBI	AGCT	2 cut(s)	127, 603
AluI	AGCT	2 cut(s)	127, 603
Alw26I	GTCTC	4 cut(s)	50, 165, 387, 696
AlwNI	CAGNNNCTG	1 cut(s)	677
ApeKI	GCWGC	1 cut(s)	612
ApoI	RAATTY	2 cut(s)	242, 275
AseI	ATTAAT	1 cut(s)	237
AspS9I	GGNCC	1 cut(s)	635
AsuHPI	GGTGA	1 cut(s)	629
AvaII	GGWCC	1 cut(s)	635
BanII	GRGCYC	1 cut(s)	536
BbvI	GCAGC	1 cut(s)	624
BccI	CCATC	3 cut(s)	164, 357, 653
BciT130I	CCWGG	2 cut(s)	222, 231
BcoDI	GTCTC	4 cut(s)	50, 165, 387, 696
BcuI	ACTAGT	1 cut(s)	331
BfaI	CTAG	3 cut(s)	23, 332, 501
BfuAI	ACCTGC	1 cut(s)	676
BglII	AGATCT	1 cut(s)	497
BisI	GCNGC	1 cut(s)	613
BlsI	GCNGC	1 cut(s)	614
Bme1390I	CCNGG	2 cut(s)	222, 231
Bme18I	GGWCC	1 cut(s)	635
BmgT120I	GGNCC	1 cut(s)	635
BmiI	GGNNCC	5 cut(s)	286, 453, 533, 705, 735
BmrFI	CCNGG	2 cut(s)	222, 231
BmsI	GCATC	2 cut(s)	417, 664
Bpu10I	CCTNAGC	1 cut(s)	192
BpuEI	CTTGAG	1 cut(s)	149
BsaI	GGTCTC	1 cut(s)	387
BsaJI	CCNNGG	3 cut(s)	229, 230, 337
BsaWI	WCCGGW	1 cut(s)	120
BsaXI	ACNNNNNCTCC	2 cut(s)	141, 171
Bsc4I	CCNNNNNNNGG	2 cut(s)	307, 358
BseBI	CCWGG	2 cut(s)	222, 231
BseDI	CCNNGG	3 cut(s)	229, 230, 337
BseGI	GGATG	3 cut(s)	5, 175, 368
BseLI	CCNNNNNNNGG	2 cut(s)	307, 358
BseMII	CTCAG	2 cut(s)	206, 389
BseRI	GAGGAG	1 cut(s)	162
BseXI	GCAGC	1 cut(s)	624
BsiSI	CCGG	1 cut(s)	121
BslI	CCNNNNNNNGG	2 cut(s)	307, 358
BsmAI	GTCTC	4 cut(s)	50, 165, 387, 696
BsmI	GAATGC	1 cut(s)	663
Bso31I	GGTCTC	1 cut(s)	387
Bsp1286I	GDGCHC	1 cut(s)	536
Bsp143I	GATC	2 cut(s)	497, 550
BspACI	CCGC	2 cut(s)	615, 731
BspCNI	CTCAG	2 cut(s)	205, 388
BspHI	TCATGA	3 cut(s)	136, 694, 724
BspLI	GGNNCC	5 cut(s)	286, 453, 533, 705, 735
BspMI	ACCTGC	1 cut(s)	676
BspTNI	GGTCTC	1 cut(s)	387
BssECI	CCNNGG	3 cut(s)	229, 230, 337
BssMI	GATC	2 cut(s)	497, 550
BssT1I	CCWWGG	1 cut(s)	337
Bst2UI	CCWGG	2 cut(s)	222, 231
Bst6I	CTCTTC	1 cut(s)	450
BstDEI	CTNAG	2 cut(s)	192, 375
BstEII	GGTNACC	1 cut(s)	224
BstF5I	GGATG	3 cut(s)	5, 175, 368
BstKTI	GATC	2 cut(s)	500, 553
BstMAI	GTCTC	4 cut(s)	50, 165, 387, 696
BstMBI	GATC	2 cut(s)	497, 550
BstMWI	GCNNNNNNNGC	1 cut(s)	609
BstNI	CCWGG	2 cut(s)	222, 231
BstPI	GGTNACC	1 cut(s)	224
BstSCI	CCNGG	2 cut(s)	220, 229
BstV1I	GCAGC	1 cut(s)	624
BstX2I	RGATCY	1 cut(s)	497
BstYI	RGATCY	1 cut(s)	497
BtsCI	GGATG	3 cut(s)	5, 175, 368
BtsIMutI	CAGTG	2 cut(s)	86, 651
BveI	ACCTGC	1 cut(s)	676
CaiI	CAGNNNCTG	1 cut(s)	677
CciI	TCATGA	3 cut(s)	136, 694, 724
Cfr13I	GGNCC	1 cut(s)	635
CviAII	CATG	6 cut(s)	137, 368, 514, 596, 695, 725
CviJI	RGCY	5 cut(s)	87, 127, 433, 534, 603
CviKI_1	RGCY	5 cut(s)	87, 127, 433, 534, 603
DdeI	CTNAG	2 cut(s)	192, 375
DpnI	GATC	2 cut(s)	499, 552
DpnII	GATC	2 cut(s)	497, 550
Eam1104I	CTCTTC	1 cut(s)	450
EarI	CTCTTC	1 cut(s)	450
Eco130I	CCWWGG	1 cut(s)	337
Eco24I	GRGCYC	1 cut(s)	536
Eco31I	GGTCTC	1 cut(s)	387
Eco32I	GATATC	1 cut(s)	218
Eco47I	GGWCC	1 cut(s)	635
Eco57I	CTGAAG	1 cut(s)	431
Eco91I	GGTNACC	1 cut(s)	224
EcoO65I	GGTNACC	1 cut(s)	224
EcoRI	GAATTC	1 cut(s)	242
EcoRII	CCWGG	2 cut(s)	220, 229
EcoRV	GATATC	1 cut(s)	218
EcoT14I	CCWWGG	1 cut(s)	337
EcoT38I	GRGCYC	1 cut(s)	536
ErhI	CCWWGG	1 cut(s)	337
FaeI	CATG	6 cut(s)	140, 371, 517, 599, 698, 728
FatI	CATG	6 cut(s)	136, 367, 513, 595, 694, 724
FauNDI	CATATG	1 cut(s)	568
Fnu4HI	GCNGC	1 cut(s)	613
FokI	GGATG	2 cut(s)	182, 375
FriOI	GRGCYC	1 cut(s)	536
Fsp4HI	GCNGC	1 cut(s)	613
FspBI	CTAG	3 cut(s)	23, 332, 501
GluI	GCNGC	1 cut(s)	613
HapII	CCGG	1 cut(s)	121
Hin1II	CATG	6 cut(s)	140, 371, 517, 599, 698, 728
HindIII	AAGCTT	1 cut(s)	125
HinfI	GANTC	8 cut(s)	11, 19, 26, 44, 133, 371, 467, 641
HpaII	CCGG	1 cut(s)	121
HphI	GGTGA	1 cut(s)	629
Hpy166II	GTNNAC	3 cut(s)	118, 403, 439
Hpy188I	TCNGA	4 cut(s)	10, 184, 378, 426
Hpy8I	GTNNAC	3 cut(s)	118, 403, 439
HpyAV	CCTTC	3 cut(s)	251, 423, 623
HpyCH4V	TGCA	2 cut(s)	575, 685
HpyF10VI	GCNNNNNNNGC	1 cut(s)	609
HpyF3I	CTNAG	2 cut(s)	192, 375
Hsp92II	CATG	6 cut(s)	140, 371, 517, 599, 698, 728
Kzo9I	GATC	2 cut(s)	497, 550
LmnI	GCTCC	1 cut(s)	531
Lsp1109I	GCAGC	1 cut(s)	624
LweI	GCATC	2 cut(s)	417, 664
MaeI	CTAG	3 cut(s)	23, 332, 501
MaeIII	GTNAC	1 cut(s)	224
MalI	GATC	2 cut(s)	499, 552
MboI	GATC	2 cut(s)	497, 550
MboII	GAAGA	4 cut(s)	169, 406, 437, 507
MflI	RGATCY	1 cut(s)	497
MhlI	GDGCHC	1 cut(s)	536
MluCI	AATT	4 cut(s)	96, 242, 275, 577
MlyI	GAGTC	5 cut(s)	13, 38, 142, 365, 461
MmeI	TCCRAC	1 cut(s)	692
MnlI	CCTC	5 cut(s)	140, 265, 372, 691, 721
MseI	TTAA	1 cut(s)	237
MslI	CAYNNNNRTG	1 cut(s)	141
MspA1I	CMGCKG	1 cut(s)	615
MspI	CCGG	1 cut(s)	121
MspR9I	CCNGG	2 cut(s)	222, 231
Mva1269I	GAATGC	1 cut(s)	663
MvaI	CCWGG	2 cut(s)	222, 231
MwoI	GCNNNNNNNGC	1 cut(s)	609
NdeI	CATATG	1 cut(s)	568
NdeII	GATC	2 cut(s)	497, 550
NlaIII	CATG	6 cut(s)	140, 371, 517, 599, 698, 728
NlaIV	GGNNCC	5 cut(s)	286, 453, 533, 705, 735
PagI	TCATGA	3 cut(s)	136, 694, 724
PaqCI	CACCTGC	1 cut(s)	676
PasI	CCCWGGG	1 cut(s)	230
PctI	GAATGC	1 cut(s)	663
PfeI	GAWTC	3 cut(s)	11, 26, 641
PflMI	CCANNNNNTGG	1 cut(s)	358
PkrI	GCNGC	1 cut(s)	614
PleI	GAGTC	5 cut(s)	13, 38, 141, 365, 461
PpsI	GAGTC	5 cut(s)	13, 38, 141, 365, 461
PshBI	ATTAAT	1 cut(s)	237
Psp6I	CCWGG	2 cut(s)	220, 229
PspEI	GGTNACC	1 cut(s)	224
PspGI	CCWGG	2 cut(s)	220, 229
PspN4I	GGNNCC	5 cut(s)	286, 453, 533, 705, 735
PspPI	GGNCC	1 cut(s)	635
PstNI	CAGNNNCTG	1 cut(s)	677
PsuI	RGATCY	1 cut(s)	497
RseI	CAYNNNNRTG	1 cut(s)	141
SaqAI	TTAA	1 cut(s)	237
SatI	GCNGC	1 cut(s)	613
Sau3AI	GATC	2 cut(s)	497, 550
Sau96I	GGNCC	1 cut(s)	635
SchI	GAGTC	5 cut(s)	13, 38, 142, 365, 461
ScrFI	CCNGG	2 cut(s)	222, 231
SduI	GDGCHC	1 cut(s)	536
SetI	ASST	8 cut(s)	64, 129, 226, 383, 605, 634, 690, 702
SfaNI	GCATC	2 cut(s)	417, 664
SinI	GGWCC	1 cut(s)	635
SmiMI	CAYNNNNRTG	1 cut(s)	141
SmlI	CTYRAG	1 cut(s)	164
SmoI	CTYRAG	1 cut(s)	164
SpeI	ACTAGT	1 cut(s)	331
Sse9I	AATT	4 cut(s)	96, 242, 275, 577
SsiI	CCGC	2 cut(s)	615, 731
SspMI	CTAG	3 cut(s)	23, 332, 501
StyD4I	CCNGG	2 cut(s)	220, 229
StyI	CCWWGG	1 cut(s)	337
TasI	AATT	4 cut(s)	96, 242, 275, 577
TfiI	GAWTC	3 cut(s)	11, 26, 641
Tru1I	TTAA	1 cut(s)	237
Tru9I	TTAA	1 cut(s)	237
TscAI	CASTG	2 cut(s)	86, 651
TseI	GCWGC	1 cut(s)	612
TspDTI	ATGAA	3 cut(s)	255, 530, 555
TspGWI	ACGGA	1 cut(s)	603
TspRI	CASTG	2 cut(s)	86, 651
Van91I	CCANNNNNTGG	1 cut(s)	358
VpaK11BI	GGWCC	1 cut(s)	635
VspI	ATTAAT	1 cut(s)	237
XapI	RAATTY	2 cut(s)	242, 275
XbaI	TCTAGA	2 cut(s)	22, 500
XspI	CTAG	3 cut(s)	23, 332, 501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.