Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003604

Physical Location & Seq

Genomic Coordinates Forward (+)

857876 .. 864614

Gene Structure 6739 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1008000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2370 bp

ATGGTGCAATCGAGACGAGAGAAGCGAAAGGAAGCTCGGTTGGCGAAGAAAGCAACAAAGCATGAGTTGTGGAGTCGACATAAGAATATTCAAAAGCTTGGTAAAGCCAATGTCAGGGATTTGAAGTCGAAAAATGTAGAGAAGAAGAAAGATTCTGTTGGTCTGACTGCGAAAAGAGTTAATTCTGAGCGAAATGAGACTAGGTTTCAATCTAATACGGTGGAAGAGAAAGTTGAAGCGAAAGCAGAGTTTCTGAAACGAGATGATCATAAGGCCTCAAAGGCGGTTAAAAGAGAAAAGGTATCTAAGAGAACCCCGAAAACGAATTTTGAAAAGTATCTTGAAATGGATAGAATTGGGGCTGAGGAGGATTTGGAATTAGAAAGGAGACTTGCAAAGAAACTCAAAGTGAAGGATGGGAAATTGAAGGGGGAGGATTTTGGCCTTAATGTGTTGTTTGAAGGGATCTTACCTGTTGATTCATTTGGCACAGAAGAAACGATATATGCTGATGCATTACCTGTTCAAAAATCTAAGAATAGCGAGTCACACAAGAAACGGAAGAAAGATAAGTCATTGGAGGGCAGGCTAGAAGGTGAGCTACCTTCTAGAGGAGTTCCAGAGGAGGCAGTAACTGATGGTGCAGAGGTGGAAATTGAAGACTTTCCTTCCAAGAAATCATCGAGGAAAAGAAATAAGAAGAGAAAGCTGATAGAGGAATCTGAAGAAGCCAAGGAAGGTGACATGATGGTTGATGTATCTGAGCAAATGGAGTCATGTGGTGCAGAGATGGCATCAGAAAAGGTTCCCATGAAGGCGCCTGAAAAATATGTTGCTCCTCAAAAGTATGTAGCTCCTCACTTAAGATCTCGTGCAGGGAATGAGCCAGAAGAGCATAGTCAGATTCGTAGACGCGTAAGAGGTCTTCTGAATAGGTTGTCCGAATCTAATGTGGAATCTATTACAGGAGACCTGTCTGCTATTTTTCGTTCTATTCCTCGTGCCATTGCTTCTCAAATGTTCAGTGAGGAGGTTATATCATCTTGTGCTGGCGGTCCTCGTGGGAATGAACAATATGCTGCTGTTTTTGCTGCTCTTGTGGCAGGCATGGCCTGTTTAGTAGGAATGGACTTTGGTGCAAACCTTTTGGCTTCACTTGCAAAAACCTTTGAGGACGAGTACCACAAAGAAGACAATATTTCGCTGAGAAATGTTACGCTTTTACTTTCATATTTATGCATATTTGGAGTTTGCTCGAGTGAATTGATATACGACTTTCTGGTTGTTTTGAGCAAGCGGTTAACCGAGGTTGATGTCTCTACCATCCTGACAGTTTTACAAAGCTGTGGAATGAAAATAAGGGCCGATGATCCTCTTGCGATGAAAAACTTTATTCAAAGTGTTCAAAATAGAGTGAATGAGCTAAAGGCCTCCTGTGGAGATAACCAGGATAATATTAACGGCAAAAGAATGGAATTCATGCTTGAGACCATATTGGACATCAAGAACAACAAAAAAAGGCCTAAAGAGGATACTGCACAGCATACTAGGATAAAGAAATGGCTACAAAAGTTACGGGTAGAAGACAGTTTAATAAGAGGGCTTAAATGGAGCAAACTTCTTGATTCTAGTAAGAAGGGCCAATGGTGGTTATCTGGGGATATTGCCTCAGCAACAGACACTGTCGAAGATGTTGCCAACACAATAGACAAAGAGGTTCTTGAAACCCAGAAAATGCTGCATATTGCTGCTGCACAGAGAATGAACACAGATGCCAGGAGGGCAATCTTTTGTATAATCATGAGTGCTGAGGATTATCTTGATGCGTTTGAGAAGCTTCTAAGATTGGATTTGCCTGGAAAGCAGGACAGGGAGATCATCAGGGTTCTTGTGGACTGTTGTTTGCAGGAGAAAGTGTTTAACAAGTATTACACCACTCTGGCTTCCAAGTTGTGTGAGCATGACAAAAATCACAAGTTCACTTTACAGTTTTGCCTTTGGGACCACTTCAAAGATCTGGAGTCGATGCCGCTTACCGGGTCAATGCACCTAGCAAAATTCGTAGCAGAGATGGTAGCCTCTTTCACCCTCTCCCTTGCAGTATTGAAGACGGTAGATCTAGTCGACATTAAGCAGCTAACAGCAAAAAGGACAATGTATTTCCGGATGCTATTCGAGGCCATCTTTGAGTATCCTGACAGCCTCATATGGAACATATTCACGCGTGTAGCTGTTTCCCCGGAGCTCGAAGGTCTTCGGCATGGGATCGAGTTTTTCATCAAATATATTGTGGAAGCCAATAGAGCCCTCAAGGAAAAGTTTAAGCTTGCAAAGAAAGCCCTGAGTAATGTTGAGGGAGTTCTGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005677 GO:0005737 GO:0006139 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008283 GO:0009507 GO:0009536 GO:0009555 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009909 GO:0009987 GO:0010026 GO:0010154 GO:0010214 GO:0010468 GO:0016043 GO:0017053 GO:0019219 GO:0019222 GO:0022414 GO:0022607 GO:0030154 GO:0031323 GO:0031461 GO:0031497 GO:0031507 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045787 GO:0046483 GO:0048229 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048580 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051239 GO:0051276 GO:0051726 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070828 GO:0071103 GO:0071514 GO:0071704 GO:0071840 GO:0080008 GO:0080090 GO:0090558 GO:0090568 GO:0099402 GO:1901360 GO:1902494 GO:1990234 GO:2000026 GO:2000241 GO:2000653

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MA3 MIF4G

Protein Analysis

790

Amino Acids

89.89

Weight (kDa)

9.0

Isoelectric Point (pI)

51.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MIF4G	PF02854	304 - 505	5.9e-21	MIF4G domain
MA3	PF02847	593 - 694	2.4e-22	MA3 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003604)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G17930
fragaria_vesca	FvH4_2g11600 FvH4_2g11600 FvH4_2g11600 FvH4_2g11600
malus_domestica	MD12G1008000.v1.1
prunus_persica	Prupe.1G541500_v2.0.a1 Prupe.1G541500_v2.0.a1 Prupe.1G541500_v2.0.a1 Prupe.1G541500_v2.0.a1 Prupe.1G541500_v2.0.a1 Prupe.1G541500_v2.0.a1 Prupe.1G541500_v2.0.a1
pyrus_communis	pycom12g00600
rosa_chinensis	RchiOBHm_Chr5g0003011 RchiOBHm_Chr5g0003131 RchiOBHm_Chr6g0270841
rosa_laevigata	RLG00000013766 RLG00000029740 RLG00000030769
rosa_multiflora	Rmu_co8456101.1_g000001 Rmu_sc0000642.1_g000028 Rmu_sc0003683.1_g000002 Rmu_sc0005956.1_g000006
rosa_roxburghii	Rroxscaffold_7G00197590
rosa_rugosa	Rorug06G0056700 Rorug06G0056800
rosa_samantha	Rh1BG088600 Rh1DG121800 Rh6AG176100 Rh6BG179300 Rh6CG175500 Rh6DG167800
rosa_wichuraiana	Rw1G009430 Rw6G015000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	817
AccI	GTMKAC	3 cut(s)	76, 910, 2124
AccII	CGCG	2 cut(s)	915, 2224
AccIII	TCCGGA	1 cut(s)	2163
AciI	CCGC	4 cut(s)	284, 1053, 1297, 2030
AclWI	GGATC	3 cut(s)	473, 1364, 2273
AcsI	RAATTY	3 cut(s)	325, 1475, 2057
AcuI	CTGAAG	1 cut(s)	744
AcyI	GRCGYC	1 cut(s)	818
AfaI	GTAC	1 cut(s)	1181
AfiI	CCNNNNNNNGG	3 cut(s)	114, 611, 2036
AflII	CTTAAG	1 cut(s)	862
AflIII	ACRYGT	2 cut(s)	913, 2222
AjnI	CCWGG	3 cut(s)	1446, 1775, 1855
Alw21I	GWGCWC	1 cut(s)	2247
Alw26I	GTCTC	6 cut(s)	7, 191, 382, 963, 1321, 1481
AlwI	GGATC	3 cut(s)	473, 1364, 2273
AlwNI	CAGNNNCTG	2 cut(s)	635, 1682
Ama87I	CYCGRG	1 cut(s)	1255
Aor13HI	TCCGGA	1 cut(s)	2163
AoxI	GGCC	8 cut(s)	273, 442, 1110, 1362, 1428, 1520, 1639, 2178
ApeKI	GCWGC	6 cut(s)	1079, 1091, 1738, 1748, 1751, 2134
ApoI	RAATTY	3 cut(s)	325, 1475, 2057
Asp700I	GAANNNNTTC	4 cut(s)	663, 804, 2216, 2253
AspLEI	GCGC	1 cut(s)	820
AspS9I	GGNCC	4 cut(s)	1055, 1362, 1639, 2002
AsuC2I	CCSGG	2 cut(s)	2038, 2240
AsuHPI	GGTGA	3 cut(s)	608, 752, 2077
AvaI	CYCGRG	1 cut(s)	1255
AvaII	GGWCC	2 cut(s)	1055, 2002
BanI	GGYRCC	1 cut(s)	817
BanII	GRGCYC	2 cut(s)	2247, 2308
BarI	GAAGNNNNNNTAC	6 cut(s)	453, 485, 585, 617, 909, 941
BauI	CACGAG	3 cut(s)	870, 999, 1059
BbsI	GAAGAC	6 cut(s)	666, 917, 1197, 1590, 2114, 2246
Bbv12I	GWGCWC	1 cut(s)	2247
BbvCI	CCTCAGC	3 cut(s)	363, 1669, 1809
BbvI	GCAGC	6 cut(s)	1066, 1078, 1725, 1735, 1738, 2146
BccI	CCATC	7 cut(s)	410, 632, 742, 784, 1331, 2065, 2189
BceAI	ACGGC	1 cut(s)	1477
BcgI	CGANNNNNNTGC	2 cut(s)	1676, 1710
BciT130I	CCWGG	3 cut(s)	1448, 1777, 1857
BciVI	GTATCC	2 cut(s)	1525, 2202
BclI	TGATCA	1 cut(s)	265
BcnI	CCSGG	2 cut(s)	2038, 2240
BcoDI	GTCTC	6 cut(s)	7, 191, 382, 963, 1321, 1481
BfaI	CTAG	7 cut(s)	201, 590, 609, 1548, 1629, 2051, 2120
BfoI	RGCGCY	1 cut(s)	821
BfrI	CTTAAG	1 cut(s)	862
BfuI	GTATCC	2 cut(s)	1525, 2202
BglI	GCCNNNNNGGC	2 cut(s)	281, 1781
BglII	AGATCT	3 cut(s)	866, 2014, 2116
BisI	GCNGC	7 cut(s)	1080, 1092, 1739, 1749, 1752, 2030, 2135
BlsI	GCNGC	7 cut(s)	1081, 1093, 1740, 1750, 1753, 2031, 2136
Bme1390I	CCNGG	5 cut(s)	1448, 1777, 1857, 2038, 2240
Bme18I	GGWCC	2 cut(s)	1055, 2002
BmeT110I	CYCGRG	1 cut(s)	1255
BmgT120I	GGNCC	4 cut(s)	1055, 1362, 1639, 2002
BmiI	GGNNCC	3 cut(s)	807, 819, 2003
BmrFI	CCNGG	5 cut(s)	1448, 1777, 1857, 2038, 2240
BmsI	GCATC	6 cut(s)	502, 803, 1762, 1813, 2016, 2157
BpiI	GAAGAC	6 cut(s)	666, 917, 1197, 1590, 2114, 2246
BpmI	CTGGAG	1 cut(s)	2039
Bpu10I	CCTNAGC	3 cut(s)	363, 1669, 1809
BpuEI	CTTGAG	2 cut(s)	1505, 2294
BpuMI	CCSGG	2 cut(s)	2038, 2240
BsaHI	GRCGYC	1 cut(s)	818
BsaI	GGTCTC	2 cut(s)	963, 1481
BsaJI	CCNNGG	3 cut(s)	732, 1305, 2238
BsaWI	WCCGGW	1 cut(s)	2163
BsaXI	ACNNNNNCTCC	2 cut(s)	1901, 1931
Bsc4I	CCNNNNNNNGG	3 cut(s)	114, 611, 2036
Bse3DI	GCAATG	1 cut(s)	1005
BseAI	TCCGGA	1 cut(s)	2163
BseBI	CCWGG	3 cut(s)	1448, 1777, 1857
BseDI	CCNNGG	3 cut(s)	732, 1305, 2238
BseGI	GGATG	3 cut(s)	421, 1323, 2172
BseLI	CCNNNNNNNGG	3 cut(s)	114, 611, 2036
BseMI	GCAATG	1 cut(s)	1005
BseMII	CTCAG	7 cut(s)	177, 354, 753, 1196, 1683, 1800, 2333
BseRI	GAGGAG	6 cut(s)	380, 627, 638, 828, 846, 1043
BseXI	GCAGC	6 cut(s)	1066, 1078, 1725, 1735, 1738, 2146
BsgI	GTGCAG	5 cut(s)	663, 804, 894, 1521, 1737
Bsh1236I	CGCG	2 cut(s)	915, 2224
BshFI	GGCC	8 cut(s)	275, 444, 1112, 1364, 1430, 1522, 1641, 2180
BshNI	GGYRCC	1 cut(s)	817
BsiHKAI	GWGCWC	1 cut(s)	2247
BsiHKCI	CYCGRG	1 cut(s)	1255
BsiSI	CCGG	3 cut(s)	2037, 2164, 2240
BslFI	GGGAC	1 cut(s)	2015
BslI	CCNNNNNNNGG	3 cut(s)	114, 611, 2036
BsmAI	GTCTC	6 cut(s)	7, 191, 382, 963, 1321, 1481
BsmBI	CGTCTC	1 cut(s)	7
BsmFI	GGGAC	1 cut(s)	2015
BsnI	GGCC	8 cut(s)	275, 444, 1112, 1364, 1430, 1522, 1641, 2180
Bso31I	GGTCTC	2 cut(s)	963, 1481
BsoBI	CYCGRG	1 cut(s)	1255
Bsp1286I	GDGCHC	2 cut(s)	2247, 2308
Bsp13I	TCCGGA	1 cut(s)	2163
Bsp143I	GATC	8 cut(s)	265, 465, 866, 1369, 1875, 2014, 2116, 2265
BspACI	CCGC	4 cut(s)	284, 1053, 1297, 2030
BspANI	GGCC	8 cut(s)	275, 444, 1112, 1364, 1430, 1522, 1641, 2180
BspCNI	CTCAG	7 cut(s)	178, 355, 754, 1197, 1682, 1801, 2334
BspEI	TCCGGA	1 cut(s)	2163
BspFNI	CGCG	2 cut(s)	915, 2224
BspHI	TCATGA	1 cut(s)	1800
BspLI	GGNNCC	3 cut(s)	807, 819, 2003
BspPI	GGATC	3 cut(s)	473, 1364, 2273
BspQI	GCTCTTC	1 cut(s)	885
BspT107I	GGYRCC	1 cut(s)	817
BspTI	CTTAAG	1 cut(s)	862
BspTNI	GGTCTC	2 cut(s)	963, 1481
BsrDI	GCAATG	1 cut(s)	1005
BssECI	CCNNGG	3 cut(s)	732, 1305, 2238
BssMI	GATC	8 cut(s)	265, 465, 866, 1369, 1875, 2014, 2116, 2265
BssNI	GRCGYC	1 cut(s)	818
BssSI	CACGAG	3 cut(s)	870, 999, 1059
BssT1I	CCWWGG	1 cut(s)	732
Bst2BI	CACGAG	3 cut(s)	870, 999, 1059
Bst2UI	CCWGG	3 cut(s)	1448, 1777, 1857
Bst4CI	ACNGT	7 cut(s)	220, 1333, 1589, 1684, 1898, 1989, 2113
Bst6I	CTCTTC	3 cut(s)	219, 695, 885
BstACI	GRCGYC	1 cut(s)	818
BstAFI	CTTAAG	1 cut(s)	862
BstC8I	GCNNGC	5 cut(s)	587, 1051, 1105, 1295, 2328
BstENI	CCTNNNNNAGG	1 cut(s)	609
BstF5I	GGATG	3 cut(s)	421, 1323, 2172
BstFNI	CGCG	2 cut(s)	915, 2224
BstH2I	RGCGCY	1 cut(s)	821
BstHHI	GCGC	1 cut(s)	820
BstKTI	GATC	8 cut(s)	268, 468, 869, 1372, 1878, 2017, 2119, 2268
BstMAI	GTCTC	6 cut(s)	7, 191, 382, 963, 1321, 1481
BstMBI	GATC	8 cut(s)	265, 465, 866, 1369, 1875, 2014, 2116, 2265
BstNI	CCWGG	3 cut(s)	1448, 1777, 1857
BstSCI	CCNGG	5 cut(s)	1446, 1775, 1855, 2036, 2238
BstUI	CGCG	2 cut(s)	915, 2224
BstV1I	GCAGC	6 cut(s)	1066, 1078, 1725, 1735, 1738, 2146
BstV2I	GAAGAC	6 cut(s)	666, 917, 1197, 1590, 2114, 2246
BstX2I	RGATCY	4 cut(s)	465, 866, 2014, 2116
BstYI	RGATCY	4 cut(s)	465, 866, 2014, 2116
BsuI	GTATCC	2 cut(s)	1525, 2202
BsuRI	GGCC	8 cut(s)	275, 444, 1112, 1364, 1430, 1522, 1641, 2180
BtgZI	GCGATG	1 cut(s)	1394
BtsCI	GGATG	3 cut(s)	421, 1323, 2172
BtsIMutI	CAGTG	2 cut(s)	1030, 1680
Cac8I	GCNNGC	5 cut(s)	587, 1051, 1105, 1295, 2328
CaiI	CAGNNNCTG	2 cut(s)	635, 1682
CciI	TCATGA	1 cut(s)	1800
CfoI	GCGC	1 cut(s)	820
Cfr13I	GGNCC	4 cut(s)	1055, 1362, 1639, 2002
CseI	GACGC	1 cut(s)	921
Csp6I	GTAC	1 cut(s)	1180
CviAII	CATG	9 cut(s)	62, 745, 777, 811, 1108, 1480, 1801, 1961, 2261
CviQI	GTAC	1 cut(s)	1180
DinI	GGCGCC	1 cut(s)	819
DpnI	GATC	8 cut(s)	267, 467, 868, 1371, 1877, 2016, 2118, 2267
DpnII	GATC	8 cut(s)	265, 465, 866, 1369, 1875, 2014, 2116, 2265
Eam1104I	CTCTTC	3 cut(s)	219, 695, 885
EarI	CTCTTC	3 cut(s)	219, 695, 885
Ecl136II	GAGCTC	1 cut(s)	2245
Eco130I	CCWWGG	1 cut(s)	732
Eco147I	AGGCCT	3 cut(s)	275, 1430, 1522
Eco24I	GRGCYC	2 cut(s)	2247, 2308
Eco31I	GGTCTC	2 cut(s)	963, 1481
Eco47I	GGWCC	2 cut(s)	1055, 2002
Eco53kI	GAGCTC	1 cut(s)	2245
Eco57I	CTGAAG	1 cut(s)	744
Eco88I	CYCGRG	1 cut(s)	1255
EcoICRI	GAGCTC	1 cut(s)	2245
EcoNI	CCTNNNNNAGG	1 cut(s)	609
EcoRI	GAATTC	1 cut(s)	1475
EcoRII	CCWGG	3 cut(s)	1446, 1775, 1855
EcoT14I	CCWWGG	1 cut(s)	732
EcoT22I	ATGCAT	2 cut(s)	517, 1241
EcoT38I	GRGCYC	2 cut(s)	2247, 2308
EgeI	GGCGCC	1 cut(s)	819
EheI	GGCGCC	1 cut(s)	819
ErhI	CCWWGG	1 cut(s)	732
Esp3I	CGTCTC	1 cut(s)	7
FaeI	CATG	9 cut(s)	65, 748, 780, 814, 1111, 1483, 1804, 1964, 2264
FalI	AAGNNNNNCTT	2 cut(s)	1704, 1736
FaqI	GGGAC	1 cut(s)	2015
FatI	CATG	9 cut(s)	61, 744, 776, 810, 1107, 1479, 1800, 1960, 2260
FauNDI	CATATG	1 cut(s)	2207
FbaI	TGATCA	1 cut(s)	265
FblI	GTMKAC	3 cut(s)	76, 910, 2124
Fnu4HI	GCNGC	7 cut(s)	1080, 1092, 1739, 1749, 1752, 2030, 2135
FokI	GGATG	3 cut(s)	428, 1310, 2179
FriOI	GRGCYC	2 cut(s)	2247, 2308
Fsp4HI	GCNGC	7 cut(s)	1080, 1092, 1739, 1749, 1752, 2030, 2135
FspBI	CTAG	7 cut(s)	201, 590, 609, 1548, 1629, 2051, 2120
GlaI	GCGC	1 cut(s)	819
GluI	GCNGC	7 cut(s)	1080, 1092, 1739, 1749, 1752, 2030, 2135
GsuI	CTGGAG	1 cut(s)	2039
HaeII	RGCGCY	1 cut(s)	821
HaeIII	GGCC	8 cut(s)	275, 444, 1112, 1364, 1430, 1522, 1641, 2180
HapII	CCGG	3 cut(s)	2037, 2164, 2240
HgaI	GACGC	1 cut(s)	921
HhaI	GCGC	1 cut(s)	820
Hin1I	GRCGYC	1 cut(s)	818
Hin1II	CATG	9 cut(s)	65, 748, 780, 814, 1111, 1483, 1804, 1964, 2264
Hin6I	GCGC	1 cut(s)	818
HinP1I	GCGC	1 cut(s)	818
HincII	GTYRAC	3 cut(s)	77, 1302, 2125
HindII	GTYRAC	3 cut(s)	77, 1302, 2125
HindIII	AAGCTT	3 cut(s)	95, 1835, 2324
HpaI	GTTAAC	1 cut(s)	1302
HpaII	CCGG	3 cut(s)	2037, 2164, 2240
HphI	GGTGA	3 cut(s)	608, 752, 2077
Hpy166II	GTNNAC	6 cut(s)	77, 911, 1302, 1894, 1980, 2125
Hpy8I	GTNNAC	6 cut(s)	77, 911, 1302, 1894, 1980, 2125
HpyCH4III	ACNGT	7 cut(s)	220, 1333, 1589, 1684, 1898, 1989, 2113
Hsp92I	GRCGYC	1 cut(s)	818
Hsp92II	CATG	9 cut(s)	65, 748, 780, 814, 1111, 1483, 1804, 1964, 2264
HspAI	GCGC	1 cut(s)	818
KasI	GGCGCC	1 cut(s)	817
Kpn2I	TCCGGA	1 cut(s)	2163
Ksp22I	TGATCA	1 cut(s)	265
KspAI	GTTAAC	1 cut(s)	1302
Kzo9I	GATC	8 cut(s)	265, 465, 866, 1369, 1875, 2014, 2116, 2265
LguI	GCTCTTC	1 cut(s)	885
LmnI	GCTCC	4 cut(s)	841, 859, 1611, 2242
Lsp1109I	GCAGC	6 cut(s)	1066, 1078, 1725, 1735, 1738, 2146
LweI	GCATC	6 cut(s)	502, 803, 1762, 1813, 2016, 2157
MaeI	CTAG	7 cut(s)	201, 590, 609, 1548, 1629, 2051, 2120
MaeIII	GTNAC	5 cut(s)	546, 631, 740, 1213, 1572
MalI	GATC	8 cut(s)	267, 467, 868, 1371, 1877, 2016, 2118, 2267
MboI	GATC	8 cut(s)	265, 465, 866, 1369, 1875, 2014, 2116, 2265
MflI	RGATCY	4 cut(s)	465, 866, 2014, 2116
MhlI	GDGCHC	2 cut(s)	2247, 2308
MluCI	AATT	9 cut(s)	181, 325, 354, 377, 422, 654, 1262, 1475, 2057
MluI	ACGCGT	2 cut(s)	913, 2222
Mly113I	GGCGCC	1 cut(s)	818
MlyI	GAGTC	4 cut(s)	82, 554, 782, 2030
Mph1103I	ATGCAT	2 cut(s)	517, 1241
MroI	TCCGGA	1 cut(s)	2163
MroXI	GAANNNNTTC	4 cut(s)	663, 804, 2216, 2253
MslI	CAYNNNNRTG	1 cut(s)	1234
MspCI	CTTAAG	1 cut(s)	862
MspI	CCGG	3 cut(s)	2037, 2164, 2240
MspR9I	CCNGG	5 cut(s)	1448, 1777, 1857, 2038, 2240
MvaI	CCWGG	3 cut(s)	1448, 1777, 1857
MvnI	CGCG	2 cut(s)	915, 2224
NarI	GGCGCC	1 cut(s)	818
NciI	CCSGG	2 cut(s)	2038, 2240
NdeI	CATATG	1 cut(s)	2207
NdeII	GATC	8 cut(s)	265, 465, 866, 1369, 1875, 2014, 2116, 2265
NlaIII	CATG	9 cut(s)	65, 748, 780, 814, 1111, 1483, 1804, 1964, 2264
NlaIV	GGNNCC	3 cut(s)	807, 819, 2003
NmuCI	GTSAC	2 cut(s)	546, 740
NsiI	ATGCAT	2 cut(s)	517, 1241
PaeR7I	CTCGAG	1 cut(s)	1255
PagI	TCATGA	1 cut(s)	1800
PceI	AGGCCT	3 cut(s)	275, 1430, 1522
PciSI	GCTCTTC	1 cut(s)	885
PcsI	WCGNNNNNNNCGW	1 cut(s)	22
PdmI	GAANNNNTTC	4 cut(s)	663, 804, 2216, 2253
PfeI	GAWTC	7 cut(s)	152, 479, 719, 904, 944, 956, 1625
PflFI	GACNNNGTC	1 cut(s)	1682
PkrI	GCNGC	7 cut(s)	1081, 1093, 1740, 1750, 1753, 2031, 2136
PleI	GAGTC	4 cut(s)	81, 553, 781, 2029
PluTI	GGCGCC	1 cut(s)	821
PpsI	GAGTC	4 cut(s)	81, 553, 781, 2029
Psp124BI	GAGCTC	1 cut(s)	2247
Psp6I	CCWGG	3 cut(s)	1446, 1775, 1855
PspGI	CCWGG	3 cut(s)	1446, 1775, 1855
PspN4I	GGNNCC	3 cut(s)	807, 819, 2003
PspPI	GGNCC	4 cut(s)	1055, 1362, 1639, 2002
PspXI	VCTCGAGB	1 cut(s)	1255
PstNI	CAGNNNCTG	2 cut(s)	635, 1682
PsuI	RGATCY	4 cut(s)	465, 866, 2014, 2116
PsyI	GACNNNGTC	1 cut(s)	1682
RsaI	GTAC	1 cut(s)	1181
RsaNI	GTAC	1 cut(s)	1180
RseI	CAYNNNNRTG	1 cut(s)	1234
SacI	GAGCTC	1 cut(s)	2247
SalI	GTCGAC	2 cut(s)	75, 2123
SapI	GCTCTTC	1 cut(s)	885
SatI	GCNGC	7 cut(s)	1080, 1092, 1739, 1749, 1752, 2030, 2135
Sau3AI	GATC	8 cut(s)	265, 465, 866, 1369, 1875, 2014, 2116, 2265
Sau96I	GGNCC	4 cut(s)	1055, 1362, 1639, 2002
SchI	GAGTC	4 cut(s)	82, 554, 782, 2030
ScrFI	CCNGG	5 cut(s)	1448, 1777, 1857, 2038, 2240
SduI	GDGCHC	2 cut(s)	2247, 2308
SfaNI	GCATC	6 cut(s)	502, 803, 1762, 1813, 2016, 2157
SfoI	GGCGCC	1 cut(s)	819
Sfr274I	CTCGAG	1 cut(s)	1255
SinI	GGWCC	2 cut(s)	1055, 2002
SlaI	CTCGAG	1 cut(s)	1255
SmiMI	CAYNNNNRTG	1 cut(s)	1234
SmlI	CTYRAG	4 cut(s)	862, 1255, 1484, 2309
SmoI	CTYRAG	4 cut(s)	862, 1255, 1484, 2309
Sse9I	AATT	9 cut(s)	181, 325, 354, 377, 422, 654, 1262, 1475, 2057
SseBI	AGGCCT	3 cut(s)	275, 1430, 1522
SsiI	CCGC	4 cut(s)	284, 1053, 1297, 2030
SspDI	GGCGCC	1 cut(s)	817
SspI	AATATT	3 cut(s)	88, 1198, 1456
SspMI	CTAG	7 cut(s)	201, 590, 609, 1548, 1629, 2051, 2120
SstI	GAGCTC	1 cut(s)	2247
StuI	AGGCCT	3 cut(s)	275, 1430, 1522
StyD4I	CCNGG	5 cut(s)	1446, 1775, 1855, 2036, 2238
StyI	CCWWGG	1 cut(s)	732
TaaI	ACNGT	7 cut(s)	220, 1333, 1589, 1684, 1898, 1989, 2113
TasI	AATT	9 cut(s)	181, 325, 354, 377, 422, 654, 1262, 1475, 2057
TauI	GCSGC	1 cut(s)	2032
TfiI	GAWTC	7 cut(s)	152, 479, 719, 904, 944, 956, 1625
TscAI	CASTG	2 cut(s)	1030, 1687
TseFI	GTSAC	2 cut(s)	546, 740
TseI	GCWGC	6 cut(s)	1079, 1091, 1738, 1748, 1751, 2134
Tsp45I	GTSAC	2 cut(s)	546, 740
TspDTI	ATGAA	9 cut(s)	471, 827, 1083, 1218, 1367, 1397, 1468, 1778, 2266
TspGWI	ACGGA	1 cut(s)	574
TspRI	CASTG	2 cut(s)	1030, 1687
Tth111I	GACNNNGTC	1 cut(s)	1682
Vha464I	CTTAAG	1 cut(s)	862
VpaK11BI	GGWCC	2 cut(s)	1055, 2002
XagI	CCTNNNNNAGG	1 cut(s)	609
XapI	RAATTY	3 cut(s)	325, 1475, 2057
XbaI	TCTAGA	1 cut(s)	608
XhoI	CTCGAG	1 cut(s)	1255
XmiI	GTMKAC	3 cut(s)	76, 910, 2124
XmnI	GAANNNNTTC	4 cut(s)	663, 804, 2216, 2253
XspI	CTAG	7 cut(s)	201, 590, 609, 1548, 1629, 2051, 2120
Zsp2I	ATGCAT	2 cut(s)	517, 1241

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.