Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0003604

Physical Location & Seq

Genomic Coordinates Reverse (-)

28596090 .. 28605000

Gene Structure 8911 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG176100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2349 bp

ATGGAGAAATCAAGACAAGAAAAGCGAAAAGAAGCTCGGTCGGCCAAAAATGCCAGAAAAAACGAGTCTTGGCTCCAAAGACAGAAATCTCAAAAGCTCAAAAGTTCAAAATCTACAGAGAAACGACAAAATTCTTCTGTTCAGAATGTGAAAGACGAAACAGTTACCCAATTGGATGCTACTTCTGGGAGAAGTAGGAATATGAAAAAAACTAGTATGGGAGTGGAAAAAGAAGTTGAAAATACTAAATCAGAGGCTCCAGAACATAATTATCCAAAACCATCAAAGGCGATGCGAGGAGACAAGGTATCAAAGAGAACCCCAAAAACAAAGTTTGAGAAGTTTCTTGAACTGGATAATACTAGGGCTGAGGAGGATTTGGAACTAGAAAGGAAACTTGCAAAGAAACTCAAAGTGAAGGGTGGAAAATTGAAGGGTGAGGATCTTGGTCTTAACATGTTGTTTGAAGGAATTTCATCTTTTGCTGATTCATTGGGAAACAAAGAAGTCGCGTATCCTGAGGCATTGCCTTCTAAAAAATCTGAAAAAAGCTCTCCAAGAAAGAAACGGAAGAAGGAAAAGTTATTACCTGATGACTTGAAGGGTGAAGATCCAAAGGTTGTCGTCACAGATGGCGCTGAGGTGGAATCTGAAGATTTTCCTTCCAAGTCATCTACAAGGAAGAGACAGAAGAAGAGAAAGTCCTTGGAGGAGACAAAAGAGGTCAACAAGGAAGGTGATATGAACATTGAGGTATCTAAGCCAATACAGTCATGTGGGACGGATATTGTGTTGGAAAAAGTTCCTGCCAAGACACCCAAAAAATATGTAGCTCCTCACTTAAGATCACGTGCAGGGAATGAGCTTGAAGAGCATAGTCAGATCCGTCGACAATTACGTGGTCTTCTAAATAGACTCTCAGAATCTAATGTGGAATCTATTACAGGGGACGTGTCAGTCCTCATGCGTTCCATTCCTCGTGGTATTGCCTCTCAGATATTTGGTGAGGAGGTATTAGCCTCATGTGTTCATGGTCCTCGTGGAAATGAACAGCATGCTGCTGTTTTTGCTGCTTTTGTGGCTGGCATGGCCAGTTCAATGGGAATCGACTTTGGTGCGAAGCTTCTGGCTTTACTTGCTAAAACTTTTGAGGATGAATACCACAAAGAGGACAATATTTCCTTGAGAAATCTTACTCTTTTTCTCTCTCAATTATGCATATTTGGAGTTTGTTCAAGTGATTTGATATACGACTTTCTGATTGTTTTGAGCAAGCGGTTAACAGAGATTGATGTCTCCACCATCTTGACAGTTTTGCAATGCTGTGGAATGAAAATAAGGGCTGATGATCCTCTTGTGATGAAAAATTTTATTGCAAGTGTTCAGAATAGAGTGAATGAATTAAAAGTCTCATGTGGAGATGACCAGGAGAAGATCAATGGCAAAAGGATGGAGTTCATGCTTGAAACCATATGTGACATCAAGAACAACAAGAAAAGGCCTAAAGAAGATACCGCACCTCATACAAGGATAAAGAAATGGCTACAAAAGTTACGTGTAGAAGACATTTTAATAAGAGGACTCAATTGGAGCAAACTTCTTGATCCTAATAAGAAGGGCCAGTGGTGGTTATCTGGGGATATTGCTTCAACAGTAGACAATGTCGAAGAGGTAGCCAACACAATAGACAAAGAGGTTCTTGAAGCCCAGAAACTTTTGCAGCTCGCTGCTGCACAGAGAATGAACACAGATGCTAGAAAGGCAATTTTTTGTATAATTATGAGTGGGGAGGATTACATTGATGCATTTGAAAAGCTTTTGAGATTGGATTTACATGGAAAGCAGGACAGAGAGATAATGCGAGTTCTTGTGGAGTGCTGTTTGCAGGAGAAAGTGTTTAACAAGTATTACACAGTTCTGGCTTCCAAATTGTGTGAGCATGACAAAAATCACAAGTTCACATTACAGTTTTGCCTTTGGGACCACTTCAAACAGCTGGACTCAATGCAGCTTAGTAGGTCAATGCACCTAGCCAAATTTGTTGCAGAGATGATAGCCTCTTTCACCCTCTCCCTCGCCGTACTGAAGACGGTAGATCTAGCTGACATCAAGCAACTAACTGCAAAACGGACAATGCATTTCCGGATGTTATTTGAGGCCATCTTTGAGTATCCAGACAGCCTGATATGGAATATATTCACGCGTGTAGCCGTCACCCCGGAGCTTGAAAGTCTTCGACATGGGATTGAATTTTTTATCAAGTATATTGTGGAAACCAATAAATCCCTCGGTGAAAAGTTTAAGCTTGCAAGGAAAGCCCTGAATAATGCTGAAGGGGTTCTCATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005677 GO:0005737 GO:0006139 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008283 GO:0009507 GO:0009536 GO:0009555 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009909 GO:0009987 GO:0010026 GO:0010154 GO:0010214 GO:0010468 GO:0016043 GO:0017053 GO:0019219 GO:0019222 GO:0022414 GO:0022607 GO:0030154 GO:0031323 GO:0031461 GO:0031497 GO:0031507 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045787 GO:0046483 GO:0048229 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048580 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051239 GO:0051276 GO:0051726 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070828 GO:0071103 GO:0071514 GO:0071704 GO:0071840 GO:0080008 GO:0080090 GO:0090558 GO:0090568 GO:0099402 GO:1901360 GO:1902494 GO:1990234 GO:2000026 GO:2000241 GO:2000653

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MA3 MIF4G

Protein Analysis

782

Amino Acids

89.03

Weight (kDa)

9.23

Isoelectric Point (pI)

42.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MIF4G	PF02854	297 - 498	3.9e-17	MIF4G domain
MA3	PF02847	586 - 687	7.9e-21	MA3 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003604)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G17930
fragaria_vesca	FvH4_2g11600 FvH4_2g11600 FvH4_2g11600 FvH4_2g11600
malus_domestica	MD12G1008000.v1.1
prunus_persica	Prupe.1G541500_v2.0.a1 Prupe.1G541500_v2.0.a1 Prupe.1G541500_v2.0.a1 Prupe.1G541500_v2.0.a1 Prupe.1G541500_v2.0.a1 Prupe.1G541500_v2.0.a1 Prupe.1G541500_v2.0.a1
pyrus_communis	pycom12g00600
rosa_chinensis	RchiOBHm_Chr5g0003011 RchiOBHm_Chr5g0003131 RchiOBHm_Chr6g0270841
rosa_laevigata	RLG00000013766 RLG00000029740 RLG00000030769
rosa_multiflora	Rmu_co8456101.1_g000001 Rmu_sc0000642.1_g000028 Rmu_sc0003683.1_g000002 Rmu_sc0005956.1_g000006
rosa_roxburghii	Rroxscaffold_7G00197590
rosa_rugosa	Rorug06G0056700 Rorug06G0056800
rosa_samantha	Rh1BG088600 Rh1DG121800 Rh6AG176100 Rh6BG179300 Rh6CG175500 Rh6DG167800
rosa_wichuraiana	Rw1G009430 Rw6G015000

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	956
AccI	GTMKAC	2 cut(s)	889, 1656
AccII	CGCG	2 cut(s)	512, 2203
AccIII	TCCGGA	1 cut(s)	2142
AciI	CCGC	2 cut(s)	1276, 1515
AclWI	GGATC	5 cut(s)	450, 605, 877, 1343, 1598
AcoI	YGGCCR	2 cut(s)	42, 1089
AcsI	RAATTY	5 cut(s)	130, 471, 1366, 2036, 2249
AcuI	CTGAAG	2 cut(s)	672, 2105
AcvI	CACGTG	1 cut(s)	851
AfaI	GTAC	1 cut(s)	2082
AfiI	CCNNNNNNNGG	1 cut(s)	1168
AflII	CTTAAG	1 cut(s)	841
AflIII	ACRYGT	4 cut(s)	456, 951, 1555, 2201
AhlI	ACTAGT	1 cut(s)	212
AjiI	CACGTC	1 cut(s)	952
AjnI	CCWGG	1 cut(s)	1425
Alw26I	GTCTC	5 cut(s)	294, 679, 707, 1300, 1414
AlwI	GGATC	5 cut(s)	450, 605, 877, 1343, 1598
Aor13HI	TCCGGA	1 cut(s)	2142
AoxI	GGCC	5 cut(s)	42, 1089, 1499, 1618, 2157
ApeKI	GCWGC	6 cut(s)	1058, 1070, 1720, 1727, 1730, 2008
ApoI	RAATTY	5 cut(s)	130, 471, 1366, 2036, 2249
Asp700I	GAANNNNTTC	5 cut(s)	657, 801, 2195, 2232, 2337
AspLEI	GCGC	1 cut(s)	638
AspS9I	GGNCC	3 cut(s)	1034, 1618, 1981
AsuC2I	CCSGG	1 cut(s)	2219
AsuHPI	GGTGA	7 cut(s)	449, 617, 749, 1016, 2056, 2206, 2303
AvaII	GGWCC	2 cut(s)	1034, 1981
AxyI	CCTNAGG	1 cut(s)	519
BalI	TGGCCA	1 cut(s)	1091
BauI	CACGAG	2 cut(s)	978, 1038
BbrPI	CACGTG	1 cut(s)	851
BbsI	GAAGAC	4 cut(s)	896, 1569, 2093, 2225
BbvCI	CCTCAGC	2 cut(s)	369, 639
BbvI	GCAGC	6 cut(s)	1045, 1057, 1714, 1717, 1732, 2020
BccI	CCATC	5 cut(s)	289, 626, 1310, 1444, 2168
BceAI	ACGGC	2 cut(s)	2063, 2195
BcgI	CGANNNNNNTGC	2 cut(s)	1097, 1131
BciT130I	CCWGG	1 cut(s)	1427
BciVI	GTATCC	2 cut(s)	525, 2181
BcnI	CCSGG	1 cut(s)	2219
BcoDI	GTCTC	5 cut(s)	294, 679, 707, 1300, 1414
BcuI	ACTAGT	1 cut(s)	212
BfaI	CTAG	6 cut(s)	213, 363, 386, 1755, 2030, 2099
BfmI	CTRYAG	1 cut(s)	114
BfoI	RGCGCY	1 cut(s)	639
BfrI	CTTAAG	1 cut(s)	841
BfuI	GTATCC	2 cut(s)	525, 2181
BglII	AGATCT	1 cut(s)	2095
BisI	GCNGC	6 cut(s)	1059, 1071, 1721, 1728, 1731, 2009
BlsI	GCNGC	6 cut(s)	1060, 1072, 1722, 1729, 1732, 2010
Bme1390I	CCNGG	2 cut(s)	1427, 2219
Bme18I	GGWCC	2 cut(s)	1034, 1981
BmgBI	CACGTC	1 cut(s)	952
BmgT120I	GGNCC	3 cut(s)	1034, 1618, 1981
BmiI	GGNNCC	3 cut(s)	74, 258, 1982
BmrFI	CCNGG	2 cut(s)	1427, 2219
BmsI	GCATC	4 cut(s)	166, 282, 1741, 1792
BpiI	GAAGAC	4 cut(s)	896, 1569, 2093, 2225
BpmI	CTGGAG	1 cut(s)	243
Bpu10I	CCTNAGC	2 cut(s)	369, 639
BpuEI	CTTGAG	1 cut(s)	1204
BpuMI	CCSGG	1 cut(s)	2219
BsaAI	YACGTR	3 cut(s)	851, 899, 1556
BsaJI	CCNNGG	3 cut(s)	705, 2217, 2287
BsaWI	WCCGGW	1 cut(s)	2142
BsaXI	ACNNNNNCTCC	4 cut(s)	291, 321, 1880, 1910
Bsc4I	CCNNNNNNNGG	1 cut(s)	1168
Bse1I	ACTGG	3 cut(s)	357, 1092, 1621
Bse21I	CCTNAGG	1 cut(s)	519
Bse3DI	GCAATG	2 cut(s)	524, 1325
BseAI	TCCGGA	1 cut(s)	2142
BseBI	CCWGG	1 cut(s)	1427
BseDI	CCNNGG	3 cut(s)	705, 2217, 2287
BseGI	GGATG	4 cut(s)	181, 1159, 1455, 2151
BseLI	CCNNNNNNNGG	1 cut(s)	1168
BseMI	GCAATG	2 cut(s)	524, 1325
BseMII	CTCAG	5 cut(s)	360, 510, 630, 933, 1007
BseNI	ACTGG	3 cut(s)	357, 1092, 1621
BseRI	GAGGAG	5 cut(s)	312, 386, 725, 825, 1022
BseXI	GCAGC	6 cut(s)	1045, 1057, 1714, 1717, 1732, 2020
BsgI	GTGCAG	2 cut(s)	873, 1716
Bsh1236I	CGCG	2 cut(s)	512, 2203
Bsh1285I	CGRYCG	1 cut(s)	41
BshFI	GGCC	5 cut(s)	44, 1091, 1501, 1620, 2159
BsiEI	CGRYCG	1 cut(s)	41
BsiSI	CCGG	2 cut(s)	2143, 2219
BslFI	GGGAC	3 cut(s)	793, 962, 1994
BslI	CCNNNNNNNGG	1 cut(s)	1168
BsmAI	GTCTC	5 cut(s)	294, 679, 707, 1300, 1414
BsmFI	GGGAC	3 cut(s)	793, 962, 1994
BsnI	GGCC	5 cut(s)	44, 1091, 1501, 1620, 2159
Bsp13I	TCCGGA	1 cut(s)	2142
Bsp143I	GATC	8 cut(s)	442, 610, 845, 882, 1348, 1434, 1603, 2095
BspACI	CCGC	2 cut(s)	1276, 1515
BspANI	GGCC	5 cut(s)	44, 1091, 1501, 1620, 2159
BspCNI	CTCAG	5 cut(s)	361, 511, 631, 932, 1006
BspEI	TCCGGA	1 cut(s)	2142
BspFNI	CGCG	2 cut(s)	512, 2203
BspLI	GGNNCC	3 cut(s)	74, 258, 1982
BspPI	GGATC	5 cut(s)	450, 605, 877, 1343, 1598
BspQI	GCTCTTC	1 cut(s)	864
BspTI	CTTAAG	1 cut(s)	841
BsrDI	GCAATG	2 cut(s)	524, 1325
BsrI	ACTGG	3 cut(s)	357, 1092, 1621
BssECI	CCNNGG	3 cut(s)	705, 2217, 2287
BssMI	GATC	8 cut(s)	442, 610, 845, 882, 1348, 1434, 1603, 2095
BssSI	CACGAG	2 cut(s)	978, 1038
BssT1I	CCWWGG	1 cut(s)	705
Bst2BI	CACGAG	2 cut(s)	978, 1038
Bst2UI	CCWGG	1 cut(s)	1427
Bst4CI	ACNGT	7 cut(s)	163, 771, 1312, 1654, 1915, 1968, 2092
Bst6I	CTCTTC	4 cut(s)	677, 689, 864, 1662
BstAFI	CTTAAG	1 cut(s)	841
BstBAI	YACGTR	3 cut(s)	851, 899, 1556
BstC8I	GCNNGC	5 cut(s)	1056, 1084, 1274, 1725, 2307
BstDEI	CTNAG	7 cut(s)	369, 519, 639, 759, 919, 993, 2012
BstF5I	GGATG	4 cut(s)	181, 1159, 1455, 2151
BstFNI	CGCG	2 cut(s)	512, 2203
BstH2I	RGCGCY	1 cut(s)	639
BstHHI	GCGC	1 cut(s)	638
BstKTI	GATC	8 cut(s)	445, 613, 848, 885, 1351, 1437, 1606, 2098
BstMAI	GTCTC	5 cut(s)	294, 679, 707, 1300, 1414
BstMBI	GATC	8 cut(s)	442, 610, 845, 882, 1348, 1434, 1603, 2095
BstMCI	CGRYCG	1 cut(s)	41
BstMWI	GCNNNNNNNGC	9 cut(s)	41, 50, 871, 1067, 1079, 1088, 1136, 1760, 2315
BstNI	CCWGG	1 cut(s)	1427
BstNSI	RCATGY	2 cut(s)	460, 1058
BstSCI	CCNGG	2 cut(s)	1425, 2217
BstSFI	CTRYAG	1 cut(s)	114
BstUI	CGCG	2 cut(s)	512, 2203
BstV1I	GCAGC	6 cut(s)	1045, 1057, 1714, 1717, 1732, 2020
BstV2I	GAAGAC	4 cut(s)	896, 1569, 2093, 2225
BstX2I	RGATCY	4 cut(s)	442, 610, 882, 2095
BstXI	CCANNNNNNTGG	1 cut(s)	1099
BstYI	RGATCY	4 cut(s)	442, 610, 882, 2095
Bsu36I	CCTNAGG	1 cut(s)	519
BsuI	GTATCC	2 cut(s)	525, 2181
BsuRI	GGCC	5 cut(s)	44, 1091, 1501, 1620, 2159
BtgZI	GCGATG	1 cut(s)	305
BtrI	CACGTC	1 cut(s)	952
BtsCI	GGATG	4 cut(s)	181, 1159, 1455, 2151
BtsIMutI	CAGTG	1 cut(s)	1628
Cac8I	GCNNGC	5 cut(s)	1056, 1084, 1274, 1725, 2307
CfoI	GCGC	1 cut(s)	638
Cfr13I	GGNCC	3 cut(s)	1034, 1618, 1981
Csp6I	GTAC	1 cut(s)	2081
CviQI	GTAC	1 cut(s)	2081
DdeI	CTNAG	7 cut(s)	369, 519, 639, 759, 919, 993, 2012
DpnI	GATC	8 cut(s)	444, 612, 847, 884, 1350, 1436, 1605, 2097
DpnII	GATC	8 cut(s)	442, 610, 845, 882, 1348, 1434, 1603, 2095
DrdI	GACNNNNNNGTC	1 cut(s)	956
DseDI	GACNNNNNNGTC	1 cut(s)	956
EaeI	YGGCCR	2 cut(s)	42, 1089
Eam1104I	CTCTTC	4 cut(s)	677, 689, 864, 1662
EarI	CTCTTC	4 cut(s)	677, 689, 864, 1662
Eco130I	CCWWGG	1 cut(s)	705
Eco147I	AGGCCT	1 cut(s)	1501
Eco47I	GGWCC	2 cut(s)	1034, 1981
Eco57I	CTGAAG	2 cut(s)	672, 2105
Eco72I	CACGTG	1 cut(s)	851
Eco81I	CCTNAGG	1 cut(s)	519
EcoRII	CCWGG	1 cut(s)	1425
EcoT14I	CCWWGG	1 cut(s)	705
EcoT22I	ATGCAT	3 cut(s)	1220, 1807, 2139
ErhI	CCWWGG	1 cut(s)	705
FalI	AAGNNNNNCTT	2 cut(s)	1683, 1715
FaqI	GGGAC	3 cut(s)	793, 962, 1994
FauNDI	CATATG	1 cut(s)	1472
FblI	GTMKAC	2 cut(s)	889, 1656
Fnu4HI	GCNGC	6 cut(s)	1059, 1071, 1721, 1728, 1731, 2009
FokI	GGATG	4 cut(s)	188, 1166, 1462, 2158
Fsp4HI	GCNGC	6 cut(s)	1059, 1071, 1721, 1728, 1731, 2009
FspBI	CTAG	6 cut(s)	213, 363, 386, 1755, 2030, 2099
GlaI	GCGC	1 cut(s)	637
GluI	GCNGC	6 cut(s)	1059, 1071, 1721, 1728, 1731, 2009
GsuI	CTGGAG	1 cut(s)	243
HaeII	RGCGCY	1 cut(s)	639
HaeIII	GGCC	5 cut(s)	44, 1091, 1501, 1620, 2159
HapII	CCGG	2 cut(s)	2143, 2219
HhaI	GCGC	1 cut(s)	638
Hin6I	GCGC	1 cut(s)	636
HinP1I	GCGC	1 cut(s)	636
HincII	GTYRAC	3 cut(s)	727, 890, 1281
HindII	GTYRAC	3 cut(s)	727, 890, 1281
HindIII	AAGCTT	3 cut(s)	1121, 1814, 2303
HinfI	GANTC	9 cut(s)	65, 488, 647, 915, 923, 935, 1104, 1581, 2000
HpaI	GTTAAC	1 cut(s)	1281
HpaII	CCGG	2 cut(s)	2143, 2219
HphI	GGTGA	7 cut(s)	449, 617, 749, 1016, 2056, 2206, 2303
Hpy166II	GTNNAC	5 cut(s)	727, 890, 1281, 1657, 1959
Hpy188I	TCNGA	9 cut(s)	144, 253, 544, 652, 882, 922, 996, 1260, 1386
Hpy8I	GTNNAC	5 cut(s)	727, 890, 1281, 1657, 1959
Hpy99I	CGWCG	1 cut(s)	891
HpyCH4III	ACNGT	7 cut(s)	163, 771, 1312, 1654, 1915, 1968, 2092
HpyCH4IV	ACGT	4 cut(s)	850, 898, 951, 1555
HpyF10VI	GCNNNNNNNGC	9 cut(s)	41, 50, 871, 1067, 1079, 1088, 1136, 1760, 2315
HpyF3I	CTNAG	7 cut(s)	369, 519, 639, 759, 919, 993, 2012
HpySE526I	ACGT	4 cut(s)	850, 898, 951, 1555
HspAI	GCGC	1 cut(s)	636
Kpn2I	TCCGGA	1 cut(s)	2142
KspAI	GTTAAC	1 cut(s)	1281
Kzo9I	GATC	8 cut(s)	442, 610, 845, 882, 1348, 1434, 1603, 2095
LguI	GCTCTTC	1 cut(s)	864
LmnI	GCTCC	5 cut(s)	78, 262, 838, 1590, 2221
Lsp1109I	GCAGC	6 cut(s)	1045, 1057, 1714, 1717, 1732, 2020
LweI	GCATC	4 cut(s)	166, 282, 1741, 1792
MaeI	CTAG	6 cut(s)	213, 363, 386, 1755, 2030, 2099
MaeII	ACGT	4 cut(s)	850, 898, 951, 1555
MaeIII	GTNAC	5 cut(s)	163, 625, 1475, 1551, 2212
MalI	GATC	8 cut(s)	444, 612, 847, 884, 1350, 1436, 1605, 2097
MboI	GATC	8 cut(s)	442, 610, 845, 882, 1348, 1434, 1603, 2095
MfeI	CAATTG	2 cut(s)	170, 1585
MflI	RGATCY	4 cut(s)	442, 610, 882, 2095
MlsI	TGGCCA	1 cut(s)	1091
MluI	ACGCGT	1 cut(s)	2201
MluNI	TGGCCA	1 cut(s)	1091
MlyI	GAGTC	4 cut(s)	74, 909, 1575, 1994
MmeI	TCCRAC	1 cut(s)	774
Mox20I	TGGCCA	1 cut(s)	1091
Mph1103I	ATGCAT	3 cut(s)	1220, 1807, 2139
MroI	TCCGGA	1 cut(s)	2142
MroXI	GAANNNNTTC	5 cut(s)	657, 801, 2195, 2232, 2337
MscI	TGGCCA	1 cut(s)	1091
MseI	TTAA	7 cut(s)	453, 842, 1280, 1403, 1571, 1899, 2301
Msp20I	TGGCCA	1 cut(s)	1091
MspA1I	CMGCKG	1 cut(s)	1996
MspCI	CTTAAG	1 cut(s)	841
MspI	CCGG	2 cut(s)	2143, 2219
MspR9I	CCNGG	2 cut(s)	1427, 2219
MunI	CAATTG	2 cut(s)	170, 1585
MvaI	CCWGG	1 cut(s)	1427
MvnI	CGCG	2 cut(s)	512, 2203
MwoI	GCNNNNNNNGC	9 cut(s)	41, 50, 871, 1067, 1079, 1088, 1136, 1760, 2315
NciI	CCSGG	1 cut(s)	2219
NdeI	CATATG	1 cut(s)	1472
NdeII	GATC	8 cut(s)	442, 610, 845, 882, 1348, 1434, 1603, 2095
NlaIV	GGNNCC	3 cut(s)	74, 258, 1982
NmuCI	GTSAC	3 cut(s)	625, 1475, 2212
NsiI	ATGCAT	3 cut(s)	1220, 1807, 2139
NspI	RCATGY	2 cut(s)	460, 1058
PaeI	GCATGC	1 cut(s)	1058
PceI	AGGCCT	1 cut(s)	1501
PciI	ACATGT	1 cut(s)	456
PciSI	GCTCTTC	1 cut(s)	864
PcsI	WCGNNNNNNNCGW	1 cut(s)	895
PdmI	GAANNNNTTC	5 cut(s)	657, 801, 2195, 2232, 2337
PfeI	GAWTC	5 cut(s)	488, 647, 923, 935, 1104
PflFI	GACNNNGTC	1 cut(s)	1661
PkrI	GCNGC	6 cut(s)	1060, 1072, 1722, 1729, 1732, 2010
PleI	GAGTC	4 cut(s)	73, 909, 1575, 1994
PmaCI	CACGTG	1 cut(s)	851
PmlI	CACGTG	1 cut(s)	851
PpsI	GAGTC	4 cut(s)	73, 909, 1575, 1994
Ppu21I	YACGTR	3 cut(s)	851, 899, 1556
PscI	ACATGT	1 cut(s)	456
Psp6I	CCWGG	1 cut(s)	1425
PspCI	CACGTG	1 cut(s)	851
PspGI	CCWGG	1 cut(s)	1425
PspN4I	GGNNCC	3 cut(s)	74, 258, 1982
PspPI	GGNCC	3 cut(s)	1034, 1618, 1981
PsuI	RGATCY	4 cut(s)	442, 610, 882, 2095
PsyI	GACNNNGTC	1 cut(s)	1661
PvuII	CAGCTG	1 cut(s)	1996
RsaI	GTAC	1 cut(s)	2082
RsaNI	GTAC	1 cut(s)	2081
SalI	GTCGAC	1 cut(s)	888
SapI	GCTCTTC	1 cut(s)	864
SaqAI	TTAA	7 cut(s)	453, 842, 1280, 1403, 1571, 1899, 2301
SatI	GCNGC	6 cut(s)	1059, 1071, 1721, 1728, 1731, 2009
Sau3AI	GATC	8 cut(s)	442, 610, 845, 882, 1348, 1434, 1603, 2095
Sau96I	GGNCC	3 cut(s)	1034, 1618, 1981
SchI	GAGTC	4 cut(s)	74, 909, 1575, 1994
ScrFI	CCNGG	2 cut(s)	1427, 2219
SfaNI	GCATC	4 cut(s)	166, 282, 1741, 1792
SfcI	CTRYAG	1 cut(s)	114
SinI	GGWCC	2 cut(s)	1034, 1981
SmlI	CTYRAG	2 cut(s)	841, 1183
SmoI	CTYRAG	2 cut(s)	841, 1183
SpeI	ACTAGT	1 cut(s)	212
SphI	GCATGC	1 cut(s)	1058
SseBI	AGGCCT	1 cut(s)	1501
SsiI	CCGC	2 cut(s)	1276, 1515
SspI	AATATT	1 cut(s)	1177
SspMI	CTAG	6 cut(s)	213, 363, 386, 1755, 2030, 2099
StuI	AGGCCT	1 cut(s)	1501
StyD4I	CCNGG	2 cut(s)	1425, 2217
StyI	CCWWGG	1 cut(s)	705
TaaI	ACNGT	7 cut(s)	163, 771, 1312, 1654, 1915, 1968, 2092
TaiI	ACGT	4 cut(s)	853, 901, 954, 1558
TaqI	TCGA	4 cut(s)	889, 1107, 1665, 2236
TaqII	GACCGA	1 cut(s)	27
TfiI	GAWTC	5 cut(s)	488, 647, 923, 935, 1104
Tru1I	TTAA	7 cut(s)	453, 842, 1280, 1403, 1571, 1899, 2301
Tru9I	TTAA	7 cut(s)	453, 842, 1280, 1403, 1571, 1899, 2301
TscAI	CASTG	1 cut(s)	1628
TseFI	GTSAC	3 cut(s)	625, 1475, 2212
TseI	GCWGC	6 cut(s)	1058, 1070, 1720, 1727, 1730, 2008
Tsp45I	GTSAC	3 cut(s)	625, 1475, 2212
TspGWI	ACGGA	4 cut(s)	583, 797, 875, 2143
TspRI	CASTG	1 cut(s)	1628
Tth111I	GACNNNGTC	1 cut(s)	1661
Vha464I	CTTAAG	1 cut(s)	841
VpaK11BI	GGWCC	2 cut(s)	1034, 1981
XapI	RAATTY	5 cut(s)	130, 471, 1366, 2036, 2249
XceI	RCATGY	2 cut(s)	460, 1058
XmiI	GTMKAC	2 cut(s)	889, 1656
XmnI	GAANNNNTTC	5 cut(s)	657, 801, 2195, 2232, 2337
XspI	CTAG	6 cut(s)	213, 363, 386, 1755, 2030, 2099
Zsp2I	ATGCAT	3 cut(s)	1220, 1807, 2139

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.