MD12G1013900.v1.1
MYB Family

Myb-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009968
Physical Location & Seq
Reverse (-)
1451055 .. 1452490
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1013900.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGGAAGGCCTCCTTGCTGTGAGAAGGTAGGAATCAAGAAAGGTCCATGGACTCCTGAGGAGGACATCGTCCTTGTTTCTTACATCCAAGAACATGGTCCTGGTAATTGGAGATCAGTTCCCACCAACACTGGGCTCTTGAGGTGCAGCAAAAGTTGCAGACTCAGATGGACTAACTACCTCAGACCGGGAATCAAGCGCGGTAACTTTACACCTCATGAAGAAGGGATGATCATTCACTTGCAAGCTTTATTGGGTAACAAATGGGCAGCAATAGCTTCCTACCTGCCACAGAGAACCGATAACGACATAAAGAACTACTGGAACACCCACTTGAAGAAGAAGCTGAAGCCATTTCAATCAGCTCTTGATCCTCATACGACGTCGAATGATCCGTTTAATGTAAATGTATCAAGGAGGAGTTTGAGTGAAGGGAGCTTAGATAATATGACAAGCCATGCCTCCTCAATTATTCTGAGGAACAACCAATCCTCCTCCTCCACATATGCCTCAAGCACCGAGAACATTTCGCGGCTCCTAGAAGGTTGGATGAGATCCTCCCCAACTCCGAAGACCTCTAGCCTCAAATATAATGCTGCATCTGATGATGATCAAGAAAAACCTAGCGGTACTAGCACAGGAAACCTAGTATCTAACGAGGAGTTTGAATCGATTTTGTCCTATGAGAACTTGAACAATGTTGCTTCTTGGGACATGTATACTTGTGATTCATCTACTTATTCTAACAAGGTGTCACAACTCAGTATTAAAGTTCCAAATGGGGCGGAAATAATTCATGATCATCATCTTCTGCGCGACGAGGATCAGAAGAATAAGCAAATTATTCGATGCGAAAGCAGTAGTACTAATTGTAACAATCCTCCATTGACATATCTTGAGAAGTGGCTGTTAGATGAAAGTGCTGGACATGTAGAAGCCGATCAGATGGTGGAATTGTCTCCAATGTCCTTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

326

Amino Acids

36.49

Weight (kDa)

6.37

Isoelectric Point (pI)

50.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 1.1e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 75 1.9e-09 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 67 - 112 8.7e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 70 - 117 9.7e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 386
Acc36I ACCTGC 1 cut(s) 294
AccI GTMKAC 1 cut(s) 719
AccII CGCG 3 cut(s) 201, 532, 816
AciI CCGC 4 cut(s) 201, 532, 627, 785
AclWI GGATC 4 cut(s) 365, 386, 549, 831
AcuI CTGAAG 1 cut(s) 368
AcyI GRCGYC 1 cut(s) 383
AfaI GTAC 2 cut(s) 631, 865
AfiI CCNNNNNNNGG 2 cut(s) 132, 187
AflIII ACRYGT 2 cut(s) 714, 928
AgsI TTSAA 4 cut(s) 337, 359, 668, 694
AjnI CCWGG 1 cut(s) 100
AloI GAACNNNNNNTCC 2 cut(s) 473, 505
AluBI AGCT 5 cut(s) 248, 278, 346, 365, 438
AluI AGCT 5 cut(s) 248, 278, 346, 365, 438
Alw26I GTCTC 1 cut(s) 963
AlwI GGATC 4 cut(s) 365, 386, 549, 831
AoxI GGCC 1 cut(s) 8
ApeKI GCWGC 3 cut(s) 147, 269, 596
Asp700I GAANNNNTTC 1 cut(s) 792
AspLEI GCGC 2 cut(s) 201, 816
AspS9I GGNCC 2 cut(s) 44, 98
AsuC2I CCSGG 1 cut(s) 189
AvaII GGWCC 2 cut(s) 44, 98
AxyI CCTNAGG 1 cut(s) 57
BanII GRGCYC 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 578
BbvI GCAGC 3 cut(s) 159, 281, 583
BccI CCATC 2 cut(s) 162, 940
BcgI CGANNNNNNTGC 2 cut(s) 827, 861
BciT130I CCWGG 1 cut(s) 102
BclI TGATCA 3 cut(s) 231, 610, 799
BcnI CCSGG 1 cut(s) 189
BcoDI GTCTC 1 cut(s) 963
BfaI CTAG 5 cut(s) 539, 579, 624, 633, 647
BfuAI ACCTGC 1 cut(s) 294
BisI GCNGC 4 cut(s) 148, 270, 533, 597
BlsI GCNGC 4 cut(s) 149, 271, 534, 598
BmcAI AGTACT 1 cut(s) 865
Bme1390I CCNGG 2 cut(s) 102, 189
Bme18I GGWCC 2 cut(s) 44, 98
BmgT120I GGNCC 2 cut(s) 44, 98
BmiI GGNNCC 1 cut(s) 536
BmrFI CCNGG 2 cut(s) 102, 189
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 2 cut(s) 608, 839
BmuI ACTGGG 1 cut(s) 141
BpiI GAAGAC 1 cut(s) 578
BpuEI CTTGAG 3 cut(s) 160, 496, 917
BpuMI CCSGG 1 cut(s) 189
Bsa29I ATCGAT 1 cut(s) 671
BsaBI GATNNNNATC 2 cut(s) 609, 804
BsaHI GRCGYC 1 cut(s) 383
BsaJI CCNNGG 2 cut(s) 47, 969
BsaXI ACNNNNNCTCC 4 cut(s) 412, 442, 476, 506
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 187
Bse1I ACTGG 2 cut(s) 136, 326
Bse21I CCTNAGG 1 cut(s) 57
Bse8I GATNNNNATC 2 cut(s) 609, 804
BseBI CCWGG 1 cut(s) 102
BseCI ATCGAT 1 cut(s) 671
BseDI CCNNGG 2 cut(s) 47, 969
BseGI GGATG 3 cut(s) 84, 234, 555
BseJI GATNNNNATC 2 cut(s) 609, 804
BseLI CCNNNNNNNGG 2 cut(s) 132, 187
BseMII CTCAG 5 cut(s) 48, 178, 196, 467, 775
BseNI ACTGG 2 cut(s) 136, 326
BseRI GAGGAG 6 cut(s) 74, 433, 454, 484, 487, 674
BseXI GCAGC 3 cut(s) 159, 281, 583
BsgI GTGCAG 1 cut(s) 166
Bsh1236I CGCG 3 cut(s) 201, 532, 816
BshFI GGCC 1 cut(s) 10
BshVI ATCGAT 1 cut(s) 671
BsiSI CCGG 1 cut(s) 188
BslFI GGGAC 1 cut(s) 725
BslI CCNNNNNNNGG 2 cut(s) 132, 187
BsmAI GTCTC 1 cut(s) 963
BsmFI GGGAC 1 cut(s) 725
BsnI GGCC 1 cut(s) 10
Bsp1286I GDGCHC 1 cut(s) 138
Bsp143I GATC 9 cut(s) 113, 231, 370, 391, 554, 610, 799, 823, 940
Bsp19I CCATGG 1 cut(s) 47
BspACI CCGC 4 cut(s) 201, 532, 627, 785
BspANI GGCC 1 cut(s) 10
BspCNI CTCAG 5 cut(s) 49, 177, 195, 468, 774
BspDI ATCGAT 1 cut(s) 671
BspFNI CGCG 3 cut(s) 201, 532, 816
BspHI TCATGA 2 cut(s) 217, 796
BspLI GGNNCC 1 cut(s) 536
BspMI ACCTGC 1 cut(s) 294
BspPI GGATC 4 cut(s) 365, 386, 549, 831
BsrI ACTGG 2 cut(s) 136, 326
BssECI CCNNGG 2 cut(s) 47, 969
BssMI GATC 9 cut(s) 113, 231, 370, 391, 554, 610, 799, 823, 940
BssNAI GTATAC 1 cut(s) 720
BssNI GRCGYC 1 cut(s) 383
BssT1I CCWWGG 2 cut(s) 47, 969
Bst1107I GTATAC 1 cut(s) 720
Bst2UI CCWGG 1 cut(s) 102
BstACI GRCGYC 1 cut(s) 383
BstAPI GCANNNNNTGC 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 6 cut(s) 57, 164, 182, 439, 476, 761
BstDSI CCRYGG 1 cut(s) 47
BstF5I GGATG 3 cut(s) 84, 234, 555
BstFNI CGCG 3 cut(s) 201, 532, 816
BstHHI GCGC 2 cut(s) 201, 816
BstKTI GATC 9 cut(s) 116, 234, 373, 394, 557, 613, 802, 826, 943
BstMAI GTCTC 1 cut(s) 963
BstMBI GATC 9 cut(s) 113, 231, 370, 391, 554, 610, 799, 823, 940
BstMWI GCNNNNNNNGC 3 cut(s) 156, 275, 633
BstNI CCWGG 1 cut(s) 102
BstNSI RCATGY 2 cut(s) 718, 932
BstSCI CCNGG 2 cut(s) 100, 187
BstUI CGCG 3 cut(s) 201, 532, 816
BstV1I GCAGC 3 cut(s) 159, 281, 583
BstV2I GAAGAC 1 cut(s) 578
BstX2I RGATCY 1 cut(s) 554
BstXI CCANNNNNNTGG 1 cut(s) 95
BstYI RGATCY 1 cut(s) 554
BstZ17I GTATAC 1 cut(s) 720
Bsu15I ATCGAT 1 cut(s) 671
Bsu36I CCTNAGG 1 cut(s) 57
BsuRI GGCC 1 cut(s) 10
BsuTUI ATCGAT 1 cut(s) 671
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 3 cut(s) 84, 234, 555
BtsIMutI CAGTG 1 cut(s) 129
BveI ACCTGC 1 cut(s) 294
Cac8I GCNNGC 1 cut(s) 246
CciI TCATGA 2 cut(s) 217, 796
CfoI GCGC 2 cut(s) 201, 816
Cfr13I GGNCC 2 cut(s) 44, 98
ClaI ATCGAT 1 cut(s) 671
Csp6I GTAC 2 cut(s) 630, 864
CviAII CATG 7 cut(s) 48, 95, 218, 458, 715, 797, 929
CviQI GTAC 2 cut(s) 630, 864
DdeI CTNAG 6 cut(s) 57, 164, 182, 439, 476, 761
DpnI GATC 9 cut(s) 115, 233, 372, 393, 556, 612, 801, 825, 942
DpnII GATC 9 cut(s) 113, 231, 370, 391, 554, 610, 799, 823, 940
EciI GGCGGA 1 cut(s) 800
Eco130I CCWWGG 2 cut(s) 47, 969
Eco147I AGGCCT 1 cut(s) 10
Eco24I GRGCYC 1 cut(s) 138
Eco47I GGWCC 2 cut(s) 44, 98
Eco57I CTGAAG 1 cut(s) 368
Eco81I CCTNAGG 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 100
EcoT14I CCWWGG 2 cut(s) 47, 969
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 2 cut(s) 47, 969
FaeI CATG 7 cut(s) 51, 98, 221, 461, 718, 800, 932
FalI AAGNNNNNCTT 1 cut(s) 30
FaqI GGGAC 1 cut(s) 725
FatI CATG 7 cut(s) 47, 94, 217, 457, 714, 796, 928
FauNDI CATATG 1 cut(s) 505
FbaI TGATCA 3 cut(s) 231, 610, 799
FblI GTMKAC 1 cut(s) 719
Fnu4HI GCNGC 4 cut(s) 148, 270, 533, 597
FokI GGATG 3 cut(s) 71, 241, 562
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 4 cut(s) 148, 270, 533, 597
FspBI CTAG 5 cut(s) 539, 579, 624, 633, 647
GlaI GCGC 2 cut(s) 200, 815
GluI GCNGC 4 cut(s) 148, 270, 533, 597
HaeIII GGCC 1 cut(s) 10
HapII CCGG 1 cut(s) 188
HhaI GCGC 2 cut(s) 201, 816
Hin1I GRCGYC 1 cut(s) 383
Hin1II CATG 7 cut(s) 51, 98, 221, 461, 718, 800, 932
Hin6I GCGC 2 cut(s) 199, 814
HinP1I GCGC 2 cut(s) 199, 814
HindIII AAGCTT 1 cut(s) 246
HinfI GANTC 6 cut(s) 33, 52, 162, 192, 668, 728
HpaII CCGG 1 cut(s) 188
Hpy166II GTNNAC 1 cut(s) 720
Hpy188I TCNGA 7 cut(s) 167, 185, 477, 570, 604, 828, 945
Hpy188III TCNNGA 8 cut(s) 37, 56, 139, 218, 368, 614, 797, 896
Hpy8I GTNNAC 1 cut(s) 720
Hpy99I CGWCG 3 cut(s) 385, 388, 821
HpyAV CCTTC 4 cut(s) 19, 218, 425, 536
HpyCH4IV ACGT 1 cut(s) 383
HpyCH4V TGCA 4 cut(s) 147, 159, 244, 599
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 275, 633
HpyF3I CTNAG 6 cut(s) 57, 164, 182, 439, 476, 761
HpySE526I ACGT 1 cut(s) 383
Hsp92I GRCGYC 1 cut(s) 383
Hsp92II CATG 7 cut(s) 51, 98, 221, 461, 718, 800, 932
HspAI GCGC 2 cut(s) 199, 814
Ksp22I TGATCA 3 cut(s) 231, 610, 799
Kzo9I GATC 9 cut(s) 113, 231, 370, 391, 554, 610, 799, 823, 940
LmnI GCTCC 2 cut(s) 435, 540
LpnPI CCDG 9 cut(s) 69, 87, 114, 117, 201, 299, 307, 624, 909
Lsp1109I GCAGC 3 cut(s) 159, 281, 583
LweI GCATC 2 cut(s) 608, 839
MaeI CTAG 5 cut(s) 539, 579, 624, 633, 647
MaeII ACGT 1 cut(s) 383
MaeIII GTNAC 4 cut(s) 203, 257, 753, 872
MalI GATC 9 cut(s) 115, 233, 372, 393, 556, 612, 801, 825, 942
MboI GATC 9 cut(s) 113, 231, 370, 391, 554, 610, 799, 823, 940
MboII GAAGA 6 cut(s) 233, 349, 352, 583, 800, 841
MflI RGATCY 1 cut(s) 554
MhlI GDGCHC 1 cut(s) 138
MluCI AATT 6 cut(s) 106, 468, 792, 840, 868, 953
MlyI GAGTC 2 cut(s) 46, 156
MmeI TCCRAC 1 cut(s) 527
MroXI GAANNNNTTC 1 cut(s) 792
MseI TTAA 2 cut(s) 399, 768
MspI CCGG 1 cut(s) 188
MspR9I CCNGG 2 cut(s) 102, 189
MvaI CCWGG 1 cut(s) 102
MvnI CGCG 3 cut(s) 201, 532, 816
MwoI GCNNNNNNNGC 3 cut(s) 156, 275, 633
NciI CCSGG 1 cut(s) 189
NcoI CCATGG 1 cut(s) 47
NdeI CATATG 1 cut(s) 505
NdeII GATC 9 cut(s) 113, 231, 370, 391, 554, 610, 799, 823, 940
NlaIII CATG 7 cut(s) 51, 98, 221, 461, 718, 800, 932
NlaIV GGNNCC 1 cut(s) 536
NmuCI GTSAC 1 cut(s) 753
NspI RCATGY 2 cut(s) 718, 932
PagI TCATGA 2 cut(s) 217, 796
PceI AGGCCT 1 cut(s) 10
PciI ACATGT 2 cut(s) 714, 928
PcsI WCGNNNNNNNCGW 1 cut(s) 392
PdmI GAANNNNTTC 1 cut(s) 792
PfeI GAWTC 4 cut(s) 33, 192, 668, 728
PflFI GACNNNGTC 1 cut(s) 68
PkrI GCNGC 4 cut(s) 149, 271, 534, 598
PleI GAGTC 2 cut(s) 46, 156
PpsI GAGTC 2 cut(s) 46, 156
PscI ACATGT 2 cut(s) 714, 928
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 536
PspPI GGNCC 2 cut(s) 44, 98
PsuI RGATCY 1 cut(s) 554
PsyI GACNNNGTC 1 cut(s) 68
RsaI GTAC 2 cut(s) 631, 865
RsaNI GTAC 2 cut(s) 630, 864
SaqAI TTAA 2 cut(s) 399, 768
SatI GCNGC 4 cut(s) 148, 270, 533, 597
Sau3AI GATC 9 cut(s) 113, 231, 370, 391, 554, 610, 799, 823, 940
Sau96I GGNCC 2 cut(s) 44, 98
ScaI AGTACT 1 cut(s) 865
SchI GAGTC 2 cut(s) 46, 156
ScrFI CCNGG 2 cut(s) 102, 189
SduI GDGCHC 1 cut(s) 138
SfaNI GCATC 2 cut(s) 608, 839
SinI GGWCC 2 cut(s) 44, 98
SmlI CTYRAG 3 cut(s) 139, 511, 896
SmoI CTYRAG 3 cut(s) 139, 511, 896
Sse9I AATT 6 cut(s) 106, 468, 792, 840, 868, 953
SseBI AGGCCT 1 cut(s) 10
SsiI CCGC 4 cut(s) 201, 532, 627, 785
SspMI CTAG 5 cut(s) 539, 579, 624, 633, 647
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 2 cut(s) 100, 187
StyI CCWWGG 2 cut(s) 47, 969
TaiI ACGT 1 cut(s) 386
TaqI TCGA 3 cut(s) 386, 671, 847
TasI AATT 6 cut(s) 106, 468, 792, 840, 868, 953
TatI WGTACW 1 cut(s) 863
TauI GCSGC 1 cut(s) 535
TfiI GAWTC 4 cut(s) 33, 192, 668, 728
Tru1I TTAA 2 cut(s) 399, 768
Tru9I TTAA 2 cut(s) 399, 768
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 1 cut(s) 753
TseI GCWGC 3 cut(s) 147, 269, 596
Tsp45I GTSAC 1 cut(s) 753
TspDTI ATGAA 4 cut(s) 234, 720, 785, 930
TspGWI ACGGA 1 cut(s) 384
TspRI CASTG 1 cut(s) 136
Tth111I GACNNNGTC 1 cut(s) 68
VpaK11BI GGWCC 2 cut(s) 44, 98
XceI RCATGY 2 cut(s) 718, 932
XmiI GTMKAC 1 cut(s) 719
XmnI GAANNNNTTC 1 cut(s) 792
XspI CTAG 5 cut(s) 539, 579, 624, 633, 647
ZraI GACGTC 1 cut(s) 384
ZrmI AGTACT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.