MD14G1011400.v1.1
MYB Family

Myb-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009968
Physical Location & Seq
Reverse (-)
1089496 .. 1090962
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1011400.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGTAAGGCCTCCTTGCTGTGAGAAGGTTGGAATCAAGAAGGGTCCGTGGACTCCAGAGGAGGACATCATCCTTGTTTCTTACATCCAAGAGCATGGTCCTGGAAATTGGAGATCAGTTCCCACCAACACTGGGTTGATGAGGTGCAGCAAAAGTTGCAGACTCAGATGGACTAACTACCTCAGACCAGGAATCAAGCGCGGTAACTTCACACCTCACGAAGAAGGGATGATCATTCACTTGCAAGCTTTATTGGGTAACAAATGGGCAGCAATAGCTTCCTACCTGCCACAGAGAACCGATAACGACATAAAGAACTACTGGAACACTCACTTGAAGAAGAAGCTGAAGAACTTTCAATCAGCTCTTGATCCTCACACGACGTCAGATGATCTGTTTAACGTAAATGTATCAAGGAGGAGTTTGAGTGAAGGGAGCTTAGATAATATGCCAAGCCATGCCTCCTCAATTATTCTGAGGAACAACCAATCCTCCTCCACATACGCCTCAAGCACCGAGAACATTTCGCGGCTCCTAGAAGGTTGGATGAGATCCTCCCCAACTCCAAAGACCTCCAGCCTCAAACACCATGCTGCATGTGACAATGATCAAGGAAAACCTAGCGGTATTAGCACAGGAAATCTAGCATCTAACGAGGAGTTTGAATCGATTCTGTCGTATGAGAACTTGAACAATGTTGCTTCGTGGGACAGGTCTACTTGTGATTCATCTACTTATTCTAACAACAAGGTGTCACAACTTAGTATTAAAGTTCCAAACAATGGGGAAATCATTCATGATCGCCATCTTCTGCGCGACGAGGATCAGAAGAGTAAGCAAATTCTTCGATGTGAAAGCAGTAGTACTAACTGTAACAATCCTCCATTGACGTTTCTTGAGAAGTGGCTGTTGGACGAAAGTGCTGGACATGTAGAAGATGATCAGATGATGGAATTGTCTCCAATGTCCTTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

326

Amino Acids

36.59

Weight (kDa)

6.83

Isoelectric Point (pI)

54.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 1.6e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 75 3.7e-10 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 67 - 112 8.7e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 70 - 118 5.6e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 386
Acc36I ACCTGC 1 cut(s) 294
AccB7I CCANNNNNTGG 1 cut(s) 782
AccI GTMKAC 1 cut(s) 716
AccII CGCG 3 cut(s) 201, 529, 816
AciI CCGC 3 cut(s) 201, 529, 624
AclWI GGATC 3 cut(s) 365, 546, 831
AcsI RAATTY 1 cut(s) 840
AcuI CTGAAG 1 cut(s) 368
AcyI GRCGYC 1 cut(s) 383
AfaI GTAC 1 cut(s) 865
AfiI CCNNNNNNNGG 2 cut(s) 132, 782
AflIII ACRYGT 1 cut(s) 928
AgsI TTSAA 4 cut(s) 337, 359, 665, 691
AjnI CCWGG 2 cut(s) 100, 187
AjuI GAANNNNNNNTTGG 2 cut(s) 893, 925
AloI GAACNNNNNNTCC 2 cut(s) 473, 505
AluBI AGCT 5 cut(s) 248, 278, 346, 365, 438
AluI AGCT 5 cut(s) 248, 278, 346, 365, 438
Alw26I GTCTC 1 cut(s) 963
AlwI GGATC 3 cut(s) 365, 546, 831
AoxI GGCC 1 cut(s) 8
ApeKI GCWGC 3 cut(s) 147, 269, 593
ApoI RAATTY 1 cut(s) 840
Asp700I GAANNNNTTC 2 cut(s) 669, 792
AspLEI GCGC 2 cut(s) 201, 816
AspS9I GGNCC 2 cut(s) 44, 98
AvaII GGWCC 2 cut(s) 44, 98
BbvI GCAGC 3 cut(s) 159, 281, 580
BccI CCATC 3 cut(s) 162, 813, 943
BcgI CGANNNNNNTGC 2 cut(s) 827, 861
BciT130I CCWGG 2 cut(s) 102, 189
BclI TGATCA 3 cut(s) 231, 607, 940
BcoDI GTCTC 1 cut(s) 963
BfaI CTAG 3 cut(s) 536, 621, 644
BfuAI ACCTGC 1 cut(s) 294
BisI GCNGC 4 cut(s) 148, 270, 530, 594
BlsI GCNGC 4 cut(s) 149, 271, 531, 595
BmcAI AGTACT 1 cut(s) 865
Bme1390I CCNGG 2 cut(s) 102, 189
Bme18I GGWCC 2 cut(s) 44, 98
BmgT120I GGNCC 2 cut(s) 44, 98
BmiI GGNNCC 2 cut(s) 45, 533
BmrFI CCNGG 2 cut(s) 102, 189
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 1 cut(s) 656
BmuI ACTGGG 1 cut(s) 141
BpmI CTGGAG 2 cut(s) 39, 559
BpuEI CTTGAG 2 cut(s) 493, 917
Bsa29I ATCGAT 1 cut(s) 668
BsaBI GATNNNNATC 1 cut(s) 804
BsaHI GRCGYC 1 cut(s) 383
BsaJI CCNNGG 2 cut(s) 47, 969
BsaXI ACNNNNNCTCC 4 cut(s) 412, 442, 476, 506
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 782
Bse1I ACTGG 2 cut(s) 136, 326
Bse8I GATNNNNATC 1 cut(s) 804
BseBI CCWGG 2 cut(s) 102, 189
BseCI ATCGAT 1 cut(s) 668
BseDI CCNNGG 2 cut(s) 47, 969
BseGI GGATG 4 cut(s) 69, 84, 234, 552
BseJI GATNNNNATC 1 cut(s) 804
BseLI CCNNNNNNNGG 2 cut(s) 132, 782
BseMII CTCAG 3 cut(s) 178, 196, 467
BseNI ACTGG 2 cut(s) 136, 326
BseRI GAGGAG 5 cut(s) 74, 433, 454, 484, 671
BseXI GCAGC 3 cut(s) 159, 281, 580
BsgI GTGCAG 1 cut(s) 166
Bsh1236I CGCG 3 cut(s) 201, 529, 816
BshFI GGCC 1 cut(s) 10
BshVI ATCGAT 1 cut(s) 668
BslFI GGGAC 1 cut(s) 722
BslI CCNNNNNNNGG 2 cut(s) 132, 782
BsmAI GTCTC 1 cut(s) 963
BsmFI GGGAC 1 cut(s) 722
BsnI GGCC 1 cut(s) 10
Bsp143I GATC 9 cut(s) 113, 231, 370, 391, 551, 607, 799, 823, 940
BspACI CCGC 3 cut(s) 201, 529, 624
BspANI GGCC 1 cut(s) 10
BspCNI CTCAG 3 cut(s) 177, 195, 468
BspDI ATCGAT 1 cut(s) 668
BspFNI CGCG 3 cut(s) 201, 529, 816
BspHI TCATGA 1 cut(s) 796
BspLI GGNNCC 2 cut(s) 45, 533
BspMI ACCTGC 1 cut(s) 294
BspPI GGATC 3 cut(s) 365, 546, 831
BsrI ACTGG 2 cut(s) 136, 326
BssECI CCNNGG 2 cut(s) 47, 969
BssMI GATC 9 cut(s) 113, 231, 370, 391, 551, 607, 799, 823, 940
BssNI GRCGYC 1 cut(s) 383
BssT1I CCWWGG 1 cut(s) 969
Bst2UI CCWGG 2 cut(s) 102, 189
Bst4CI ACNGT 1 cut(s) 872
Bst6I CTCTTC 1 cut(s) 824
BstACI GRCGYC 1 cut(s) 383
BstAPI GCANNNNNTGC 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 5 cut(s) 164, 182, 439, 476, 761
BstDSI CCRYGG 1 cut(s) 47
BstF5I GGATG 4 cut(s) 69, 84, 234, 552
BstFNI CGCG 3 cut(s) 201, 529, 816
BstHHI GCGC 2 cut(s) 201, 816
BstKTI GATC 9 cut(s) 116, 234, 373, 394, 554, 610, 802, 826, 943
BstMAI GTCTC 1 cut(s) 963
BstMBI GATC 9 cut(s) 113, 231, 370, 391, 551, 607, 799, 823, 940
BstMWI GCNNNNNNNGC 3 cut(s) 156, 275, 630
BstNI CCWGG 2 cut(s) 102, 189
BstNSI RCATGY 2 cut(s) 600, 932
BstSCI CCNGG 2 cut(s) 100, 187
BstUI CGCG 3 cut(s) 201, 529, 816
BstV1I GCAGC 3 cut(s) 159, 281, 580
BstX2I RGATCY 1 cut(s) 551
BstXI CCANNNNNNTGG 1 cut(s) 95
BstYI RGATCY 1 cut(s) 551
Bsu15I ATCGAT 1 cut(s) 668
BsuRI GGCC 1 cut(s) 10
BsuTUI ATCGAT 1 cut(s) 668
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 4 cut(s) 69, 84, 234, 552
BtsIMutI CAGTG 1 cut(s) 129
BveI ACCTGC 1 cut(s) 294
Cac8I GCNNGC 1 cut(s) 246
CciI TCATGA 1 cut(s) 796
CfoI GCGC 2 cut(s) 201, 816
Cfr13I GGNCC 2 cut(s) 44, 98
ClaI ATCGAT 1 cut(s) 668
Csp6I GTAC 1 cut(s) 864
CviAII CATG 6 cut(s) 95, 458, 590, 597, 797, 929
CviQI GTAC 1 cut(s) 864
DdeI CTNAG 5 cut(s) 164, 182, 439, 476, 761
DpnI GATC 9 cut(s) 115, 233, 372, 393, 553, 609, 801, 825, 942
DpnII GATC 9 cut(s) 113, 231, 370, 391, 551, 607, 799, 823, 940
Eam1104I CTCTTC 1 cut(s) 824
EarI CTCTTC 1 cut(s) 824
Eco130I CCWWGG 1 cut(s) 969
Eco147I AGGCCT 1 cut(s) 10
Eco47I GGWCC 2 cut(s) 44, 98
Eco57I CTGAAG 1 cut(s) 368
EcoRII CCWGG 2 cut(s) 100, 187
EcoT14I CCWWGG 1 cut(s) 969
ErhI CCWWGG 1 cut(s) 969
FaeI CATG 6 cut(s) 98, 461, 593, 600, 800, 932
FalI AAGNNNNNCTT 1 cut(s) 30
FaqI GGGAC 1 cut(s) 722
FatI CATG 6 cut(s) 94, 457, 589, 596, 796, 928
FbaI TGATCA 3 cut(s) 231, 607, 940
FblI GTMKAC 1 cut(s) 716
Fnu4HI GCNGC 4 cut(s) 148, 270, 530, 594
FokI GGATG 4 cut(s) 56, 71, 241, 559
Fsp4HI GCNGC 4 cut(s) 148, 270, 530, 594
FspBI CTAG 3 cut(s) 536, 621, 644
GlaI GCGC 2 cut(s) 200, 815
GluI GCNGC 4 cut(s) 148, 270, 530, 594
GsuI CTGGAG 2 cut(s) 39, 559
HaeIII GGCC 1 cut(s) 10
HhaI GCGC 2 cut(s) 201, 816
Hin1I GRCGYC 1 cut(s) 383
Hin1II CATG 6 cut(s) 98, 461, 593, 600, 800, 932
Hin6I GCGC 2 cut(s) 199, 814
HinP1I GCGC 2 cut(s) 199, 814
HindIII AAGCTT 1 cut(s) 246
HinfI GANTC 7 cut(s) 33, 52, 162, 192, 665, 670, 725
Hpy166II GTNNAC 2 cut(s) 51, 717
Hpy188I TCNGA 6 cut(s) 167, 185, 388, 477, 828, 945
Hpy188III TCNNGA 6 cut(s) 37, 56, 218, 368, 797, 896
Hpy8I GTNNAC 2 cut(s) 51, 717
Hpy99I CGWCG 2 cut(s) 385, 821
HpyAV CCTTC 5 cut(s) 19, 34, 218, 425, 533
HpyCH4III ACNGT 1 cut(s) 872
HpyCH4IV ACGT 3 cut(s) 383, 402, 890
HpyCH4V TGCA 4 cut(s) 147, 159, 244, 596
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 275, 630
HpyF3I CTNAG 5 cut(s) 164, 182, 439, 476, 761
HpySE526I ACGT 3 cut(s) 383, 402, 890
Hsp92I GRCGYC 1 cut(s) 383
Hsp92II CATG 6 cut(s) 98, 461, 593, 600, 800, 932
HspAI GCGC 2 cut(s) 199, 814
Ksp22I TGATCA 3 cut(s) 231, 607, 940
Kzo9I GATC 9 cut(s) 113, 231, 370, 391, 551, 607, 799, 823, 940
LmnI GCTCC 2 cut(s) 435, 537
Lsp1109I GCAGC 3 cut(s) 159, 281, 580
LweI GCATC 1 cut(s) 656
MaeI CTAG 3 cut(s) 536, 621, 644
MaeII ACGT 3 cut(s) 383, 402, 890
MaeIII GTNAC 5 cut(s) 203, 257, 599, 753, 872
MalI GATC 9 cut(s) 115, 233, 372, 393, 553, 609, 801, 825, 942
MboI GATC 9 cut(s) 113, 231, 370, 391, 551, 607, 799, 823, 940
MboII GAAGA 8 cut(s) 233, 349, 352, 361, 800, 836, 841, 947
MflI RGATCY 1 cut(s) 551
MluCI AATT 4 cut(s) 106, 468, 840, 953
MlyI GAGTC 2 cut(s) 46, 156
MmeI TCCRAC 3 cut(s) 10, 524, 891
MroXI GAANNNNTTC 2 cut(s) 669, 792
MseI TTAA 2 cut(s) 399, 768
MspR9I CCNGG 2 cut(s) 102, 189
MvaI CCWGG 2 cut(s) 102, 189
MvnI CGCG 3 cut(s) 201, 529, 816
MwoI GCNNNNNNNGC 3 cut(s) 156, 275, 630
NdeII GATC 9 cut(s) 113, 231, 370, 391, 551, 607, 799, 823, 940
NlaIII CATG 6 cut(s) 98, 461, 593, 600, 800, 932
NlaIV GGNNCC 2 cut(s) 45, 533
NmuCI GTSAC 2 cut(s) 599, 753
NspI RCATGY 2 cut(s) 600, 932
PagI TCATGA 1 cut(s) 796
PceI AGGCCT 1 cut(s) 10
PciI ACATGT 1 cut(s) 928
PcsI WCGNNNNNNNCGW 1 cut(s) 674
PdmI GAANNNNTTC 2 cut(s) 669, 792
PfeI GAWTC 5 cut(s) 33, 192, 665, 670, 725
PflMI CCANNNNNTGG 1 cut(s) 782
PfoI TCCNGGA 1 cut(s) 100
PkrI GCNGC 4 cut(s) 149, 271, 531, 595
PleI GAGTC 2 cut(s) 46, 156
PpsI GAGTC 2 cut(s) 46, 156
PscI ACATGT 1 cut(s) 928
Psp6I CCWGG 2 cut(s) 100, 187
PspGI CCWGG 2 cut(s) 100, 187
PspN4I GGNNCC 2 cut(s) 45, 533
PspPI GGNCC 2 cut(s) 44, 98
PsuI RGATCY 1 cut(s) 551
RsaI GTAC 1 cut(s) 865
RsaNI GTAC 1 cut(s) 864
SaqAI TTAA 2 cut(s) 399, 768
SatI GCNGC 4 cut(s) 148, 270, 530, 594
Sau3AI GATC 9 cut(s) 113, 231, 370, 391, 551, 607, 799, 823, 940
Sau96I GGNCC 2 cut(s) 44, 98
ScaI AGTACT 1 cut(s) 865
SchI GAGTC 2 cut(s) 46, 156
ScrFI CCNGG 2 cut(s) 102, 189
SfaNI GCATC 1 cut(s) 656
SinI GGWCC 2 cut(s) 44, 98
SmlI CTYRAG 2 cut(s) 508, 896
SmoI CTYRAG 2 cut(s) 508, 896
Sse9I AATT 4 cut(s) 106, 468, 840, 953
SseBI AGGCCT 1 cut(s) 10
SsiI CCGC 3 cut(s) 201, 529, 624
SspMI CTAG 3 cut(s) 536, 621, 644
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 2 cut(s) 100, 187
StyI CCWWGG 1 cut(s) 969
TaaI ACNGT 1 cut(s) 872
TaiI ACGT 3 cut(s) 386, 405, 893
TaqI TCGA 2 cut(s) 668, 847
TasI AATT 4 cut(s) 106, 468, 840, 953
TatI WGTACW 1 cut(s) 863
TauI GCSGC 1 cut(s) 532
TfiI GAWTC 5 cut(s) 33, 192, 665, 670, 725
Tru1I TTAA 2 cut(s) 399, 768
Tru9I TTAA 2 cut(s) 399, 768
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 2 cut(s) 599, 753
TseI GCWGC 3 cut(s) 147, 269, 593
Tsp45I GTSAC 2 cut(s) 599, 753
TspDTI ATGAA 2 cut(s) 717, 785
TspGWI ACGGA 1 cut(s) 36
TspRI CASTG 1 cut(s) 136
Van91I CCANNNNNTGG 1 cut(s) 782
VpaK11BI GGWCC 2 cut(s) 44, 98
XapI RAATTY 1 cut(s) 840
XceI RCATGY 2 cut(s) 600, 932
XmiI GTMKAC 1 cut(s) 716
XmnI GAANNNNTTC 2 cut(s) 669, 792
XspI CTAG 3 cut(s) 536, 621, 644
ZraI GACGTC 1 cut(s) 384
ZrmI AGTACT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.