Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0008031

Physical Location & Seq

Genomic Coordinates Forward (+)

3419743 .. 3429440

Gene Structure 9698 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1030800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2751 bp

ATGGCATGGACGGAGAAAGATGTTGGTAATGTGAGAAATGGAGAGCCGGCCGGAGAGAATGGATTCTTGGATGGTTCGCAGCCTTCTCCTAGTACCAGTGGTTCTATAGTTGTTGGAGTTCCGGCAGGGAAGGTGTTTGAAGTGAAAGATGCTCTTTCTTATGCCAACATTCTTCGTTCGAGGAACAAGTTTTCTGATGCTCTAAGTCTTTACGAGACTCTGTTGGAGAAAGATCCCGGAAACGTGGAGGCTCATATTGGAAAAGGAATATGCTTGCAGATGAAGAATATGGGAAGGCTTGCTTTTGACAGTTTCGCAGAAGCCATCAGGTTGGACCCACAGAATGCGTGCGCTCACACACATTGTGGAATTTTGTACAAAGACGAGGGTCGTCTAAGGGAAGCTGCCGAGTCTTATCAAAAAGCTCTAAAAGCAGATCAATCCTACAAACCTGCTGCGGAATGCCTAGCCATTGTATTAACCGATCTTGGAACAAGCTTAAAGCTTGCCGGAAATACTCAAGAGGGACTGCAGAAGTATTATGAAGCTTTGAAGACAGATCCGCACTATGCTCCTGCATATTATAACCTTGGTGTTGTCTACTCCGAAATGATGCAATTTGACATGGCCCTTACTTGCTATGAGAAGGCTGCATTGGAGAGGCCGATGTATGCTGAAGCATATTGCAACATGGGTGTCATTTACAAGAATCGTGGTGACTTGGAGTCTGCTATTGCTTGCTACGAGAGGTGTCTTGCTGTTTCGCCAAACTTTGAGATTGCAAAGAATAATATGGCAATAGCATTGACAGATTTAGGAACAAAGGTTAAATTGGAAGGAGACATTGATCAAGGCATATCCTATTACAAGAAGGCTTTGTATTATAACTGGCATTATGCTGATGCTATGTACAATCTTGGGGTTGCTTATGGTGAAATGCTTAAATTTGACATGGCAATTGTGTTCTATGAACTTGCTTTCCACTTCAATCCCCATTGTGCAGAAGCATGCAATAATTTGGGAGTAATATACAAAGATCGAGACAACCTTGATAAAGCAGTAGAGTGTTATCAGTTGGCTTTGTCAATCAAACCAAACTTCTCACAATCGTTGAACAATCTTGGTGTTGTCTACACTGTTCAGGGTAAAATGGATGCTGCTGCAAACATGATTGACAAAGCTATAATTGCAAATCCCACATATGCAGAAGCATACAACAACTTAGGGGTACTCTACCGAGATGCTGGAAATATTACTCTCGCTATTGGTGCATATGAGGAATGCCTCAAAATAGATCCTGATTCCCGAAACGCAGGCCAGAATCGATTGCTTGCAATGAACTACATAAATGAAGGACTTGATGATAAACTTTTTGTCGCTCACAGGGACTGGGGTAGGCGCTTTATGAGGTTATATCCACAGTACACTTCATGGGACAATCCAAAAGATCCAGAACGACCCCTTGTGATTGGATACGTGTCTCCTGATTATTTTACGCATTCTGTTTCATATTTCATAGAAGCTCCGCTTGCCCACCATGAATACACAAAGTACAAGGTGGTTGTGTATTCTGCAGTAGTGAAGGCAGACGCCAAGACTATTAGGTTTCGGGAGAAAGTCTTGAAAAAGGGTGGGATTTGGAGAGATATATATGGGATTGATGAAAAGAAGGTTGCAAGCATTATTAGAGAAGATAAGGTTGATATCCTGGTGGAACTTACTGGCCATACAGCAAACAATAAATTGGGAACAATGGCATGCAGGCCCTCACCTGTTCAGGTGACCTGGATTGGCTACCCAAACACAACTGGTTTGCCGGCAATTGATTATAGAATCACAGATTCTCTGGCCGATCCTCCTGATTCAAAGCAGAAGCATGTTGAGGAGTTGGTTCGATTACCCGATTGCTTTCTTTGTTACACACCTTCCCCTGAGGCAGGGCCTGTTATGCCCACTCCTGCTCTTTCCAATGGCTTTGTTACTTTTGGTAGCTTTAATAATCTGGCGAAGATCACTCCAAAAGTGCTACAAGTTTGGGCAAGAATTCTTTCTGCAATTCCGAATTCTCGCCTTGTGGTGAAGTGTAAGCCTTTTAGTTGTGATAGTGTGAGAGAGAGATTTCTTTCAACATTGGAGCAGCTGGGGTTGGAACCTCTACGTGTTGATCTTCTGCCTCTAATTTTACTCAACTACGATCATATGCAAGCCTATTCTCTCATGGACATTAGTTTGGATACTTTTCCATATGCTGGAACAACCACAACATGTGAATCCTTATACATGGGAGTCCCGTGTGTTACTATGGCTGGTTCAGTACATGCACACAACGTTGGTGTTAGTATTCTCAGCAAAGTTGGGTTGGGAAATCTGATTGCTAAAAGTGAGGATGAATATGTCCAGTTGGCAGTGCAGCTGGCCTCTGACATCACAGCGCTTTCGAATTTGAGAATGGGTCTCCGGGACCTTATGGCCAGGTCACCTGTCTGTGATGGACCAAAGTTTACCTTTGGGCTGGAGTCTGCATACAGGAGCATGTGGCACAGTTACTGCAAAGGTGATGTGCCATCACAACGGCAAATGGAAATGCTACAAGAGGAAGTAATCGCTGAAGATCCAGCTGCTGGAGTCTCTGATCCAACAAGTATTACAACACCAAGAGAAGGCCCTCCTGGATCTATTAAGACTAATGGATTTGGTCCTCTTCCACAGCCTGTGCTTAACCCCTCTACTTGTGAAGAGAATGGAGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0007154 GO:0007165 GO:0008150 GO:0008194 GO:0008375 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009739 GO:0009740 GO:0009755 GO:0009937 GO:0009938 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010476 GO:0010646 GO:0010648 GO:0016262 GO:0016740 GO:0016757 GO:0016758 GO:0019222 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0032870 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071368 GO:0071370 GO:0071396 GO:0071495 GO:0140096 GO:1901700 GO:1901701 GO:2000377

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_transf_41 TPR_1 TPR_10 TPR_16 TPR_8

Protein Analysis

917

Amino Acids

101.43

Weight (kDa)

5.5

Isoelectric Point (pI)

38.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_1	PF00515	191 - 217	2.7e-07	Tetratricopeptide repeat
TPR_1	PF00515	225 - 258	8.3e-10	Tetratricopeptide repeat
TPR_2	PF07719	225 - 257	8.5e-07	Tetratricopeptide repeat
TPR_8	PF13181	225 - 257	6.9e-08	Tetratricopeptide repeat
TPR_12	PF13424	225 - 293	1.4e-06	Tetratricopeptide repeat
TPR_1	PF00515	334 - 367	4.3e-09	Tetratricopeptide repeat
TPR_2	PF07719	334 - 366	2.2e-06	Tetratricopeptide repeat
TPR_8	PF13181	334 - 366	1e-06	Tetratricopeptide repeat
TPR_11	PF13414	343 - 381	5.9e-06	TPR repeat
TPR_12	PF13424	369 - 432	1.6e-06	Tetratricopeptide repeat
TPR_16	PF13432	380 - 435	5.8e-07	Tetratricopeptide repeat
TPR_1	PF00515	402 - 434	5.6e-08	Tetratricopeptide repeat
TPR_2	PF07719	402 - 434	2.7e-06	Tetratricopeptide repeat
TPR_8	PF13181	402 - 434	6.6e-06	Tetratricopeptide repeat
Glyco_transf_41	PF13844	482 - 638	1.6e-23	Glycosyl transferase family 41
Glyco_transf_41	PF13844	655 - 834	2.7e-24	Glycosyl transferase family 41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008031)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11540 AT3G11540
fragaria_vesca	FvH4_6g19470 FvH4_6g19470 FvH4_6g19470 FvH4_6g19470
malus_domestica	MD12G1030800.v1.1 MD14G1031300.v1.1
prunus_persica	Prupe.7G101600_v2.0.a1 Prupe.7G101600_v2.0.a1
pyrus_communis	pycom12g02880 pycom14g02820
rosa_chinensis	RchiOBHm_Chr3g0474801
rosa_laevigata	RLG00000023896
rosa_multiflora	Rmu_sc0004848.1_g000005
rosa_roxburghii	Rroxscaffold_6G00406850
rosa_rugosa	Rorug03G0143700 Rorug03G0143800 Rorug03G0143900
rosa_samantha	Rh3AG193600 Rh3BG223400 Rh3CG218300 Rh3DG219400
rosa_wichuraiana	Rw3G017640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	585, 885
Acc36I	ACCTGC	1 cut(s)	460
AccB7I	CCANNNNNTGG	2 cut(s)	2249, 2621
AccI	GTMKAC	2 cut(s)	600, 1131
AciI	CCGC	3 cut(s)	458, 563, 1526
AclI	AACGTT	1 cut(s)	2328
AclWI	GGATC	8 cut(s)	227, 554, 1289, 1442, 1847, 2606, 2627, 2680
AcoI	YGGCCR	4 cut(s)	48, 1723, 1848, 2469
AcsI	RAATTY	5 cut(s)	369, 944, 2043, 2062, 2440
AcuI	CTGAAG	2 cut(s)	696, 2628
AcyI	GRCGYC	1 cut(s)	1590
AdeI	CACNNNGTG	1 cut(s)	365
AfaI	GTAC	7 cut(s)	94, 377, 911, 1230, 1424, 1553, 2316
AfeI	AGCGCT	1 cut(s)	2433
AfiI	CCNNNNNNNGG	4 cut(s)	1937, 2249, 2621, 2660
AflIII	ACRYGT	3 cut(s)	1476, 2158, 2264
AgsI	TTSAA	7 cut(s)	140, 553, 988, 1114, 1624, 1866, 2127
AhdI	GACNNNNNGTC	1 cut(s)	724
AjnI	CCWGG	4 cut(s)	1707, 1784, 2471, 2668
AjuI	GAANNNNNNNTTGG	4 cut(s)	50, 82, 638, 670
AloI	GAACNNNNNNTCC	2 cut(s)	963, 995
Alw26I	GTCTC	6 cut(s)	209, 834, 1035, 1485, 2459, 2632
AlwI	GGATC	8 cut(s)	227, 554, 1289, 1442, 1847, 2606, 2627, 2680
AlwNI	CAGNNNCTG	4 cut(s)	1389, 1943, 2547, 2621
Aor51HI	AGCGCT	1 cut(s)	2433
ApeKI	GCWGC	9 cut(s)	79, 404, 455, 650, 1157, 1160, 2137, 2410, 2618
ApoI	RAATTY	5 cut(s)	369, 944, 2043, 2062, 2440
Asp700I	GAANNNNTTC	2 cut(s)	62, 2047
AspLEI	GCGC	3 cut(s)	353, 1401, 2434
AspS9I	GGNCC	8 cut(s)	334, 628, 1764, 1940, 2461, 2492, 2663, 2696
AsuC2I	CCSGG	2 cut(s)	237, 2459
AsuHPI	GGTGA	7 cut(s)	728, 944, 1761, 1792, 2089, 2469, 2567
AsuII	TTCGAA	1 cut(s)	2438
AvaII	GGWCC	4 cut(s)	334, 2461, 2492, 2696
AxyI	CCTNAGG	1 cut(s)	1932
BalI	TGGCCA	2 cut(s)	1725, 2471
BbsI	GAAGAC	1 cut(s)	560
BbvI	GCAGC	9 cut(s)	91, 391, 442, 637, 1144, 1147, 2149, 2422, 2605
BccI	CCATC	4 cut(s)	65, 332, 2483, 2572
BceAI	ACGGC	1 cut(s)	2588
BciT130I	CCWGG	4 cut(s)	1709, 1786, 2473, 2670
BciVI	GTATCC	2 cut(s)	1466, 2227
BclI	TGATCA	1 cut(s)	847
BcnI	CCSGG	2 cut(s)	237, 2459
BcoDI	GTCTC	6 cut(s)	209, 834, 1035, 1485, 2459, 2632
BfaI	CTAG	2 cut(s)	90, 467
BfmI	CTRYAG	3 cut(s)	105, 530, 1572
BfoI	RGCGCY	2 cut(s)	1402, 2435
BfuAI	ACCTGC	1 cut(s)	460
BfuI	GTATCC	2 cut(s)	1466, 2227
BisI	GCNGC	9 cut(s)	80, 405, 456, 651, 1158, 1161, 2138, 2411, 2619
BlsI	GCNGC	9 cut(s)	81, 406, 457, 652, 1159, 1162, 2139, 2412, 2620
Bme1390I	CCNGG	6 cut(s)	237, 1709, 1786, 2459, 2473, 2670
Bme18I	GGWCC	4 cut(s)	334, 2461, 2492, 2696
BmeRI	GACNNNNNGTC	1 cut(s)	724
BmgT120I	GGNCC	8 cut(s)	334, 628, 1764, 1940, 2461, 2492, 2663, 2696
BmiI	GGNNCC	3 cut(s)	336, 2151, 2462
BmrFI	CCNGG	6 cut(s)	237, 1709, 1786, 2459, 2473, 2670
BmrI	ACTGGG	1 cut(s)	1399
BmsI	GCATC	6 cut(s)	139, 187, 603, 892, 1144, 1231
BmuI	ACTGGG	1 cut(s)	1399
BoxI	GACNNNNGTC	1 cut(s)	387
BpiI	GAAGAC	1 cut(s)	560
BpmI	CTGGAG	2 cut(s)	2534, 2643
Bpu14I	TTCGAA	1 cut(s)	2438
BpuEI	CTTGAG	1 cut(s)	504
BpuMI	CCSGG	2 cut(s)	237, 2459
Bsa29I	ATCGAT	1 cut(s)	1324
BsaAI	YACGTR	2 cut(s)	1477, 2159
BsaHI	GRCGYC	1 cut(s)	1590
BsaI	GGTCTC	1 cut(s)	2459
BsaJI	CCNNGG	1 cut(s)	589
Bsc4I	CCNNNNNNNGG	4 cut(s)	1937, 2249, 2621, 2660
Bse118I	RCCGGY	2 cut(s)	46, 1816
Bse1I	ACTGG	6 cut(s)	96, 893, 1394, 1726, 1813, 2398
Bse21I	CCTNAGG	1 cut(s)	1932
Bse3DI	GCAATG	1 cut(s)	1341
BseBI	CCWGG	4 cut(s)	1709, 1786, 2473, 2670
BseCI	ATCGAT	1 cut(s)	1324
BseDI	CCNNGG	1 cut(s)	589
BseGI	GGATG	3 cut(s)	76, 1159, 2392
BseLI	CCNNNNNNNGG	4 cut(s)	1937, 2249, 2621, 2660
BseMI	GCAATG	1 cut(s)	1341
BseMII	CTCAG	2 cut(s)	1923, 2359
BseNI	ACTGG	6 cut(s)	96, 893, 1394, 1726, 1813, 2398
BseRI	GAGGAG	1 cut(s)	1898
BseX3I	CGGCCG	1 cut(s)	48
BseXI	GCAGC	9 cut(s)	91, 391, 442, 637, 1144, 1147, 2149, 2422, 2605
BseYI	CCCAGC	1 cut(s)	2140
BsgI	GTGCAG	2 cut(s)	1020, 2429
Bsh1285I	CGRYCG	1 cut(s)	51
BshVI	ATCGAT	1 cut(s)	1324
BsiEI	CGRYCG	1 cut(s)	51
BsiSI	CCGG	7 cut(s)	47, 51, 122, 237, 510, 1817, 2458
BslFI	GGGAC	5 cut(s)	540, 1400, 1448, 2273, 2474
BslI	CCNNNNNNNGG	4 cut(s)	1937, 2249, 2621, 2660
BsmAI	GTCTC	6 cut(s)	209, 834, 1035, 1485, 2459, 2632
BsmFI	GGGAC	5 cut(s)	540, 1400, 1448, 2273, 2474
BsmI	GAATGC	4 cut(s)	349, 467, 1286, 1498
Bso31I	GGTCTC	1 cut(s)	2459
Bsp119I	TTCGAA	1 cut(s)	2438
Bsp1407I	TGTACA	2 cut(s)	375, 909
BspACI	CCGC	3 cut(s)	458, 563, 1526
BspCNI	CTCAG	2 cut(s)	1924, 2358
BspDI	ATCGAT	1 cut(s)	1324
BspLI	GGNNCC	3 cut(s)	336, 2151, 2462
BspMAI	CTGCAG	2 cut(s)	534, 1576
BspMI	ACCTGC	1 cut(s)	460
BspPI	GGATC	8 cut(s)	227, 554, 1289, 1442, 1847, 2606, 2627, 2680
BspT104I	TTCGAA	1 cut(s)	2438
BspTNI	GGTCTC	1 cut(s)	2459
BsrDI	GCAATG	1 cut(s)	1341
BsrFI	RCCGGY	2 cut(s)	46, 1816
BsrGI	TGTACA	2 cut(s)	375, 909
BsrI	ACTGG	6 cut(s)	96, 893, 1394, 1726, 1813, 2398
BssAI	RCCGGY	2 cut(s)	46, 1816
BssECI	CCNNGG	1 cut(s)	589
BssNI	GRCGYC	1 cut(s)	1590
BssT1I	CCWWGG	1 cut(s)	589
Bst2UI	CCWGG	4 cut(s)	1709, 1786, 2473, 2670
Bst4CI	ACNGT	4 cut(s)	311, 1138, 1422, 2543
Bst6I	CTCTTC	2 cut(s)	2706, 2730
BstACI	GRCGYC	1 cut(s)	1590
BstAUI	TGTACA	2 cut(s)	375, 909
BstBAI	YACGTR	2 cut(s)	1477, 2159
BstBI	TTCGAA	1 cut(s)	2438
BstDEI	CTNAG	5 cut(s)	203, 395, 1222, 1932, 2345
BstEII	GGTNACC	2 cut(s)	1780, 2475
BstENI	CCTNNNNNAGG	1 cut(s)	1935
BstF5I	GGATG	3 cut(s)	76, 1159, 2392
BstH2I	RGCGCY	2 cut(s)	1402, 2435
BstHHI	GCGC	3 cut(s)	353, 1401, 2434
BstMAI	GTCTC	6 cut(s)	209, 834, 1035, 1485, 2459, 2632
BstMCI	CGRYCG	1 cut(s)	51
BstMWI	GCNNNNNNNGC	5 cut(s)	431, 1187, 1268, 1529, 1948
BstNI	CCWGG	4 cut(s)	1709, 1786, 2473, 2670
BstNSI	RCATGY	6 cut(s)	1011, 1761, 1880, 2268, 2321, 2536
BstPAI	GACNNNNGTC	1 cut(s)	387
BstPI	GGTNACC	2 cut(s)	1780, 2475
BstSCI	CCNGG	6 cut(s)	235, 1707, 1784, 2457, 2471, 2668
BstSFI	CTRYAG	3 cut(s)	105, 530, 1572
BstV1I	GCAGC	9 cut(s)	91, 391, 442, 637, 1144, 1147, 2149, 2422, 2605
BstV2I	GAAGAC	1 cut(s)	560
BstX2I	RGATCY	6 cut(s)	232, 559, 1294, 1447, 2611, 2672
BstXI	CCANNNNNNTGG	1 cut(s)	331
BstYI	RGATCY	6 cut(s)	232, 559, 1294, 1447, 2611, 2672
BstZI	CGGCCG	1 cut(s)	48
Bsu15I	ATCGAT	1 cut(s)	1324
Bsu36I	CCTNAGG	1 cut(s)	1932
BsuI	GTATCC	2 cut(s)	1466, 2227
BsuTUI	ATCGAT	1 cut(s)	1324
BtsCI	GGATG	3 cut(s)	76, 1159, 2392
BtsI	GCAGTG	1 cut(s)	2412
BtsIMutI	CAGTG	3 cut(s)	103, 1134, 2412
BveI	ACCTGC	1 cut(s)	460
CaiI	CAGNNNCTG	4 cut(s)	1389, 1943, 2547, 2621
CfoI	GCGC	3 cut(s)	353, 1401, 2434
Cfr10I	RCCGGY	2 cut(s)	46, 1816
Cfr13I	GGNCC	8 cut(s)	334, 628, 1764, 1940, 2461, 2492, 2663, 2696
ClaI	ATCGAT	1 cut(s)	1324
CseI	GACGC	1 cut(s)	1598
Csp6I	GTAC	7 cut(s)	93, 376, 910, 1229, 1423, 1552, 2315
CspCI	CAANNNNNGTGG	2 cut(s)	694, 729
CviQI	GTAC	7 cut(s)	93, 376, 910, 1229, 1423, 1552, 2315
DdeI	CTNAG	5 cut(s)	203, 395, 1222, 1932, 2345
DraIII	CACNNNGTG	1 cut(s)	365
DriI	GACNNNNNGTC	1 cut(s)	724
EaeI	YGGCCR	4 cut(s)	48, 1723, 1848, 2469
EagI	CGGCCG	1 cut(s)	48
Eam1104I	CTCTTC	2 cut(s)	2706, 2730
Eam1105I	GACNNNNNGTC	1 cut(s)	724
EarI	CTCTTC	2 cut(s)	2706, 2730
EclXI	CGGCCG	1 cut(s)	48
Eco130I	CCWWGG	1 cut(s)	589
Eco31I	GGTCTC	1 cut(s)	2459
Eco32I	GATATC	1 cut(s)	1705
Eco47I	GGWCC	4 cut(s)	334, 2461, 2492, 2696
Eco47III	AGCGCT	1 cut(s)	2433
Eco52I	CGGCCG	1 cut(s)	48
Eco57I	CTGAAG	2 cut(s)	696, 2628
Eco81I	CCTNAGG	1 cut(s)	1932
Eco91I	GGTNACC	2 cut(s)	1780, 2475
EcoNI	CCTNNNNNAGG	1 cut(s)	1935
EcoO109I	RGGNCCY	4 cut(s)	1764, 1940, 2461, 2663
EcoO65I	GGTNACC	2 cut(s)	1780, 2475
EcoRI	GAATTC	2 cut(s)	2043, 2062
EcoRII	CCWGG	4 cut(s)	1707, 1784, 2471, 2668
EcoRV	GATATC	1 cut(s)	1705
EcoT14I	CCWWGG	1 cut(s)	589
ErhI	CCWWGG	1 cut(s)	589
FalI	AAGNNNNNCTT	8 cut(s)	138, 170, 286, 318, 1512, 1544, 2489, 2521
FaqI	GGGAC	5 cut(s)	540, 1400, 1448, 2273, 2474
FauNDI	CATATG	4 cut(s)	1201, 1273, 2199, 2245
FbaI	TGATCA	1 cut(s)	847
FblI	GTMKAC	2 cut(s)	600, 1131
Fnu4HI	GCNGC	9 cut(s)	80, 405, 456, 651, 1158, 1161, 2138, 2411, 2619
FokI	GGATG	3 cut(s)	83, 1166, 2399
Fsp4HI	GCNGC	9 cut(s)	80, 405, 456, 651, 1158, 1161, 2138, 2411, 2619
FspBI	CTAG	2 cut(s)	90, 467
GlaI	GCGC	3 cut(s)	352, 1400, 2433
GluI	GCNGC	9 cut(s)	80, 405, 456, 651, 1158, 1161, 2138, 2411, 2619
GsaI	CCCAGC	1 cut(s)	2144
GsuI	CTGGAG	2 cut(s)	2534, 2643
HaeII	RGCGCY	2 cut(s)	1402, 2435
HapII	CCGG	7 cut(s)	47, 51, 122, 237, 510, 1817, 2458
HgaI	GACGC	1 cut(s)	1598
HhaI	GCGC	3 cut(s)	353, 1401, 2434
Hin1I	GRCGYC	1 cut(s)	1590
Hin6I	GCGC	3 cut(s)	351, 1399, 2432
HinP1I	GCGC	3 cut(s)	351, 1399, 2432
HindIII	AAGCTT	3 cut(s)	496, 503, 546
HpaII	CCGG	7 cut(s)	47, 51, 122, 237, 510, 1817, 2458
HphI	GGTGA	7 cut(s)	728, 944, 1761, 1792, 2089, 2469, 2567
Hpy166II	GTNNAC	4 cut(s)	601, 1132, 1425, 2502
Hpy188I	TCNGA	7 cut(s)	196, 607, 2061, 2370, 2422, 2632, 2750
Hpy188III	TCNNGA	9 cut(s)	521, 1040, 1298, 1305, 1451, 1484, 1610, 1621, 1859
Hpy8I	GTNNAC	4 cut(s)	601, 1132, 1425, 2502
HpyCH4III	ACNGT	4 cut(s)	311, 1138, 1422, 2543
HpyCH4IV	ACGT	4 cut(s)	243, 1476, 2158, 2328
HpyF10VI	GCNNNNNNNGC	5 cut(s)	431, 1187, 1268, 1529, 1948
HpyF3I	CTNAG	5 cut(s)	203, 395, 1222, 1932, 2345
HpySE526I	ACGT	4 cut(s)	243, 1476, 2158, 2328
Hsp92I	GRCGYC	1 cut(s)	1590
HspAI	GCGC	3 cut(s)	351, 1399, 2432
KroI	GCCGGC	2 cut(s)	46, 1816
KroNI	GCCGGC	2 cut(s)	48, 1818
Ksp22I	TGATCA	1 cut(s)	847
LmnI	GCTCC	4 cut(s)	577, 1528, 2134, 2529
Lsp1109I	GCAGC	9 cut(s)	91, 391, 442, 637, 1144, 1147, 2149, 2422, 2605
LweI	GCATC	6 cut(s)	139, 187, 603, 892, 1144, 1231
MaeI	CTAG	2 cut(s)	90, 467
MaeII	ACGT	4 cut(s)	243, 1476, 2158, 2328
MaeIII	GTNAC	7 cut(s)	716, 1780, 1916, 1978, 2296, 2475, 2543
MboII	GAAGA	9 cut(s)	164, 295, 565, 1703, 2020, 2159, 2621, 2693, 2747
MfeI	CAATTG	2 cut(s)	957, 1821
MflI	RGATCY	6 cut(s)	232, 559, 1294, 1447, 2611, 2672
MlsI	TGGCCA	2 cut(s)	1725, 2471
MluNI	TGGCCA	2 cut(s)	1725, 2471
MlyI	GAGTC	6 cut(s)	211, 419, 734, 2295, 2525, 2634
MmeI	TCCRAC	5 cut(s)	94, 204, 312, 2127, 2660
Mox20I	TGGCCA	2 cut(s)	1725, 2471
MroNI	GCCGGC	2 cut(s)	46, 1816
MroXI	GAANNNNTTC	2 cut(s)	62, 2047
MscI	TGGCCA	2 cut(s)	1725, 2471
MseI	TTAA	7 cut(s)	479, 500, 828, 942, 1995, 2679, 2718
Msp20I	TGGCCA	2 cut(s)	1725, 2471
MspA1I	CMGCKG	3 cut(s)	2140, 2413, 2618
MspI	CCGG	7 cut(s)	47, 51, 122, 237, 510, 1817, 2458
MspR9I	CCNGG	6 cut(s)	237, 1709, 1786, 2459, 2473, 2670
MunI	CAATTG	2 cut(s)	957, 1821
Mva1269I	GAATGC	4 cut(s)	349, 467, 1286, 1498
MvaI	CCWGG	4 cut(s)	1709, 1786, 2473, 2670
MwoI	GCNNNNNNNGC	5 cut(s)	431, 1187, 1268, 1529, 1948
NaeI	GCCGGC	2 cut(s)	48, 1818
NciI	CCSGG	2 cut(s)	237, 2459
NdeI	CATATG	4 cut(s)	1201, 1273, 2199, 2245
NgoMIV	GCCGGC	2 cut(s)	46, 1816
NlaIV	GGNNCC	3 cut(s)	336, 2151, 2462
NmeAIII	GCCGAG	1 cut(s)	433
NmuCI	GTSAC	3 cut(s)	716, 1780, 2475
NspI	RCATGY	6 cut(s)	1011, 1761, 1880, 2268, 2321, 2536
NspV	TTCGAA	1 cut(s)	2438
PaeI	GCATGC	2 cut(s)	1011, 1761
PciI	ACATGT	1 cut(s)	2264
PctI	GAATGC	4 cut(s)	349, 467, 1286, 1498
PdiI	GCCGGC	2 cut(s)	48, 1818
PdmI	GAANNNNTTC	2 cut(s)	62, 2047
PfeI	GAWTC	8 cut(s)	63, 709, 1301, 1321, 1833, 1841, 1862, 2270
PflMI	CCANNNNNTGG	2 cut(s)	2249, 2621
PfoI	TCCNGGA	3 cut(s)	235, 2457, 2668
PkrI	GCNGC	9 cut(s)	81, 406, 457, 652, 1159, 1162, 2139, 2412, 2620
PleI	GAGTC	6 cut(s)	211, 418, 733, 2294, 2524, 2633
PpsI	GAGTC	6 cut(s)	211, 418, 733, 2294, 2524, 2633
Ppu21I	YACGTR	2 cut(s)	1477, 2159
PpuMI	RGGWCCY	1 cut(s)	2461
PscI	ACATGT	1 cut(s)	2264
PshAI	GACNNNNGTC	1 cut(s)	387
PsiI	TTATAA	2 cut(s)	585, 885
Psp1406I	AACGTT	1 cut(s)	2328
Psp5II	RGGWCCY	1 cut(s)	2461
Psp6I	CCWGG	4 cut(s)	1707, 1784, 2471, 2668
PspEI	GGTNACC	2 cut(s)	1780, 2475
PspFI	CCCAGC	1 cut(s)	2140
PspGI	CCWGG	4 cut(s)	1707, 1784, 2471, 2668
PspN4I	GGNNCC	3 cut(s)	336, 2151, 2462
PspPI	GGNCC	8 cut(s)	334, 628, 1764, 1940, 2461, 2492, 2663, 2696
PspPPI	RGGWCCY	1 cut(s)	2461
PsrI	GAACNNNNNNTAC	2 cut(s)	85, 117
PstI	CTGCAG	2 cut(s)	534, 1576
PstNI	CAGNNNCTG	4 cut(s)	1389, 1943, 2547, 2621
PsuI	RGATCY	6 cut(s)	232, 559, 1294, 1447, 2611, 2672
PvuII	CAGCTG	3 cut(s)	2140, 2413, 2618
RsaI	GTAC	7 cut(s)	94, 377, 911, 1230, 1424, 1553, 2316
RsaNI	GTAC	7 cut(s)	93, 376, 910, 1229, 1423, 1552, 2315
SaqAI	TTAA	7 cut(s)	479, 500, 828, 942, 1995, 2679, 2718
SatI	GCNGC	9 cut(s)	80, 405, 456, 651, 1158, 1161, 2138, 2411, 2619
Sau96I	GGNCC	8 cut(s)	334, 628, 1764, 1940, 2461, 2492, 2663, 2696
SchI	GAGTC	6 cut(s)	211, 419, 734, 2295, 2525, 2634
ScrFI	CCNGG	6 cut(s)	237, 1709, 1786, 2459, 2473, 2670
SfaNI	GCATC	6 cut(s)	139, 187, 603, 892, 1144, 1231
SfcI	CTRYAG	3 cut(s)	105, 530, 1572
SfuI	TTCGAA	1 cut(s)	2438
SinI	GGWCC	4 cut(s)	334, 2461, 2492, 2696
SmlI	CTYRAG	1 cut(s)	519
SmoI	CTYRAG	1 cut(s)	519
SphI	GCATGC	2 cut(s)	1011, 1761
SsiI	CCGC	3 cut(s)	458, 563, 1526
SspI	AATATT	1 cut(s)	1252
SspMI	CTAG	2 cut(s)	90, 467
StyD4I	CCNGG	6 cut(s)	235, 1707, 1784, 2457, 2471, 2668
StyI	CCWWGG	1 cut(s)	589
TaaI	ACNGT	4 cut(s)	311, 1138, 1422, 2543
TaiI	ACGT	4 cut(s)	246, 1479, 2161, 2331
TaqI	TCGA	5 cut(s)	179, 1039, 1324, 1894, 2438
TatI	WGTACW	5 cut(s)	375, 909, 1422, 1551, 2314
TfiI	GAWTC	8 cut(s)	63, 709, 1301, 1321, 1833, 1841, 1862, 2270
Tru1I	TTAA	7 cut(s)	479, 500, 828, 942, 1995, 2679, 2718
Tru9I	TTAA	7 cut(s)	479, 500, 828, 942, 1995, 2679, 2718
TscAI	CASTG	3 cut(s)	103, 1141, 2412
TseFI	GTSAC	3 cut(s)	716, 1780, 2475
TseI	GCWGC	9 cut(s)	79, 404, 455, 650, 1157, 1160, 2137, 2410, 2618
Tsp45I	GTSAC	3 cut(s)	716, 1780, 2475
TspGWI	ACGGA	1 cut(s)	26
TspRI	CASTG	3 cut(s)	103, 1141, 2412
Van91I	CCANNNNNTGG	2 cut(s)	2249, 2621
VpaK11BI	GGWCC	4 cut(s)	334, 2461, 2492, 2696
XagI	CCTNNNNNAGG	1 cut(s)	1935
XapI	RAATTY	5 cut(s)	369, 944, 2043, 2062, 2440
XceI	RCATGY	6 cut(s)	1011, 1761, 1880, 2268, 2321, 2536
XmiI	GTMKAC	2 cut(s)	600, 1131
XmnI	GAANNNNTTC	2 cut(s)	62, 2047
XspI	CTAG	2 cut(s)	90, 467

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.