Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0008031

Physical Location & Seq

Genomic Coordinates Forward (+)

20560048 .. 20570168

Gene Structure 10121 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ44034

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2751 bp

ATGGCTTGGACGGATAAGGATGGTTGTAATGGGAAAGAAGGGGACCCAGTTGTAGAGAATGGATTCTTGAACTGTTCGCAGCCTTCTCCTAGTACCAGTGGCTCTCTTCTTGCTGGTGCAACTCCCGAAGGGGGGAAGAGTTTTGAAGTGAAAGAGACGATTTCTTATGCCAACATTCTTCGTTCAAGGAACAAGTTTTCTGATGCTCTTAGTCTTTATGAGACTGTGTTGGACAAAGATGCCAGAAATGTGGAAGCTCATATTGGGAAAGGAATATGCTTGCAAATGAAGAATATGGGAAGGCTTGCTTTTGACAGTTTCACGGAGGCCATCAAGTTGGACTCAGAGAATGCATGTGCCCTCACGCATTGTGGTATTTTGTACAAAGATGAGGGCCGCCTAAGGGAGGCTGCTGAGTCGTATCAAAAGGCTCTGAATGCAGACCCTTCATACAAACCCGCTGCTGAATGCCTAGCCATTGTATTAACAGATCTTGGAACTAGTTTAAAGCTTGCTGGCAACACTCAAGATGGATTGCAGAAGTATTATGAAGCTTTGAAGACAGATCCACACTATGCTCCTGCTTATTATAACCTTGGTGTTGTCTACTCTGAAATGATGCAATTTGACACGGCGCTTACTTGCTATGAGAAGGCTGCATTGGAGAGGCCGATGTATGCTGAAGCATATTGCAACATGGGCGTTATTTACAAAAATCGTGGCGACTTGGAGTCTGCTATTGCTTGTTACGAGAGGTGTCTAGCTGTTTCGCCAAATTTTGAGATTGCAAAGAATAATATGGCAATAGCCTTGACAGATTTAGGAACAAAGGTTAAATTGGAGGGAGATATCGATCAAGGCATAGCATATTACAAGAAGGCTTTGTATTATAACTGGCACTATGCTGATGCTATGTACAATCTTGGGGTTGCTTATGGTGAAATGCTTAAGTTTGACATGGCAATTGTGTTCTATGAACTTGCTTTCCACTTCAATCCCCATTGTGCAGAGGCGTGCAATAATTTGGGAGTAATATACAAAGACAGAGACAACCTTGATAAAGCAGTGGAGTGTTATCAGATGGCTTTGTCAATCAAACCAAACTTCTCACAGTCATTGAACAATCTTGGAGTTGTCTACACTGTTCAGGGTAAGATGGATGCTGCTGCAAGCATGATTGAGAAAGCTATAATTGCAAATCCCACATATGCAGAAGCATATAACAACCTAGGGGTTCTTTACAGAGATGCTGGCAATATTTCTCTGGCTATTGATGCATATGAGCAATGCCTTAAAATAGATCCTGATTCACGAAATGCAGGCCAGAACCGGTTGCTTGCAATGAACTACATTCATGAAGGACAAGATGATAAACTTTTTGTGGCTCACAGGGACTGGGGTAGACGTTTTATGAGGCTATTCTCACAGCACACTTCTTGGGACAATCTAAAAGATCCGGAACGTCCCTTAGTGATTGGATATGTATCTCCCGATTATTTTACTCACTCTGTATCATATTTTATAGAAGCTCCTCTTGCCCACCATGAATATGCAAAATACAAAGTGGTTGTTTATTCTGCAGTTGTGAAGGCAGATGCTAAGACTATCAGGTTTAGGGACAAAGTCTTGAAAAAGGGTGGGATCTGGAGAGATATTTATGGGATTGATGAAAAAAAAGTCGCAAGCATTGTTAGGGAAGATAAGATTGATATCTTGGTGGAACTTACTGGTCATACTGCTAACAACAAGTTGGGAACAATGGCATGCCGGCCTGCACCTGTTCAGGTTACTTGGATTGGTTACCCAAACACAACTGGTTTGCCAGCAATTGATTATAGAATCACAGATTCACTGGCAGATTCTCCTGATTCAAAGCAGAAGCATGTTGAGCAGCTAGTTCGATTACCAGAATGCTTCCTTTGTTATACACCTTCCCCTGAGGCAGGGCCTGTTTCCCCTACTCCAGCTCTTTCCAATGGCTTTATTACATTTGGAAGTTTTAATAATCTCGCAAAGATCACACCAAAAGTGCTGCAAGTTTGGGCAAGAATTCTCTCAGCAATTCCAAATTCTCGCCTTGTAGTAAAATGTAAGCCCTTTAGTTGCGATAGTGTGAGAGAGAGATTTCTTTCAACATTGGAGCAGCTGGGGTTGGAACCACTACGTGTTGATCTTCTGCCTCTAATTTTACTCAATTACGATCATATGCAAGCATATTCTCTCATGGACATTAGTTTGGATACTTTTCCTTATGCTGGAACAACCACGACATGTGAATCTTTATATATGGGCGTTCCATGTGTTACTATGGCTGGTTCAGTACATGCACACAATGTTGGTGTCAGTATTCTCAGCACAGTGGGATTGGGAAACCTTATTGCTAAAAATGAGGAAGAATATGTTCAGTTGGCTGTGCAGTTGGCCTCTGACATAACAGCACTTTCAAATCTGAGGATGAGTCTGCGAGACCTCATGTCCAGGTCGCCTGTCTGTGATGGACCAAAGTTTACCCTTGGGCTGGAGTCGGCATACAGGAACATGTGGGGCAGGTACTGCAAAGGTGATGTGCCATCACAACGGCACATGGAAATACTACAACAGGAGGTAACCCCTGAAGAGCCAACTACCAAAATTGCTGAACCAATAAGGATTACCGAATCAGGTTTTCCTGCATCGATCAAGTCCAACGGATTTAATCCTTGTCCAACACCTATGGCTAATCTCTCTTCTAGTGAAGAAAATGGAGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0007154 GO:0007165 GO:0008150 GO:0008194 GO:0008375 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009739 GO:0009740 GO:0009755 GO:0009937 GO:0009938 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010476 GO:0010646 GO:0010648 GO:0016262 GO:0016740 GO:0016757 GO:0016758 GO:0019222 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0032870 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071368 GO:0071370 GO:0071396 GO:0071495 GO:0140096 GO:1901700 GO:1901701 GO:2000377

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_transf_41 TPR_1 TPR_10 TPR_16 TPR_7 TPR_8

Protein Analysis

916

Amino Acids

101.61

Weight (kDa)

5.59

Isoelectric Point (pI)

41.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_1	PF00515	193 - 219	6.4e-07	Tetratricopeptide repeat
TPR_1	PF00515	227 - 260	8.3e-10	Tetratricopeptide repeat
TPR_2	PF07719	227 - 259	8.5e-07	Tetratricopeptide repeat
TPR_8	PF13181	227 - 259	6.9e-08	Tetratricopeptide repeat
TPR_12	PF13424	227 - 295	1e-06	Tetratricopeptide repeat
TPR_1	PF00515	336 - 369	9.2e-10	Tetratricopeptide repeat
TPR_2	PF07719	336 - 368	3.6e-06	Tetratricopeptide repeat
TPR_11	PF13414	345 - 383	2.9e-06	TPR repeat
TPR_12	PF13424	371 - 434	1.9e-08	Tetratricopeptide repeat
TPR_16	PF13432	382 - 437	1.1e-06	Tetratricopeptide repeat
TPR_1	PF00515	404 - 436	1.6e-09	Tetratricopeptide repeat
TPR_2	PF07719	404 - 436	4.4e-07	Tetratricopeptide repeat
TPR_8	PF13181	404 - 436	2.9e-07	Tetratricopeptide repeat
Glyco_transf_41	PF13844	483 - 640	5.8e-23	Glycosyl transferase family 41
Glyco_transf_41	PF13844	657 - 836	1.5e-25	Glycosyl transferase family 41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008031)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11540 AT3G11540
fragaria_vesca	FvH4_6g19470 FvH4_6g19470 FvH4_6g19470 FvH4_6g19470
malus_domestica	MD12G1030800.v1.1 MD14G1031300.v1.1
prunus_persica	Prupe.7G101600_v2.0.a1 Prupe.7G101600_v2.0.a1
pyrus_communis	pycom12g02880 pycom14g02820
rosa_chinensis	RchiOBHm_Chr3g0474801
rosa_laevigata	RLG00000023896
rosa_multiflora	Rmu_sc0004848.1_g000005
rosa_roxburghii	Rroxscaffold_6G00406850
rosa_rugosa	Rorug03G0143700 Rorug03G0143800 Rorug03G0143900
rosa_samantha	Rh3AG193600 Rh3BG223400 Rh3CG218300 Rh3DG219400
rosa_wichuraiana	Rw3G017640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	591, 891
Acc36I	ACCTGC	1 cut(s)	2538
AccI	GTMKAC	3 cut(s)	606, 1137, 1402
AccIII	TCCGGA	1 cut(s)	1456
AciI	CCGC	2 cut(s)	397, 459
AclWI	GGATC	4 cut(s)	560, 1295, 1448, 1649
AcsI	RAATTY	3 cut(s)	775, 2049, 2068
AcuI	CTGAAG	2 cut(s)	702, 2634
AdeI	CACNNNGTG	1 cut(s)	2165
AfaI	GTAC	5 cut(s)	94, 383, 917, 2322, 2552
AfiI	CCNNNNNNNGG	7 cut(s)	131, 132, 403, 406, 1943, 2255, 2518
AflII	CTTAAG	1 cut(s)	947
AflIII	ACRYGT	3 cut(s)	2164, 2270, 2538
AgeI	ACCGGT	1 cut(s)	1329
AhdI	GACNNNNNGTC	2 cut(s)	730, 2473
AhlI	ACTAGT	1 cut(s)	500
AjnI	CCWGG	1 cut(s)	2477
AjuI	GAANNNNNNNTTGG	4 cut(s)	246, 278, 644, 676
AloI	GAACNNNNNNTCC	2 cut(s)	969, 1001
AluBI	AGCT	9 cut(s)	257, 511, 554, 764, 1187, 1529, 1894, 1967, 2146
AluI	AGCT	9 cut(s)	257, 511, 554, 764, 1187, 1529, 1894, 1967, 2146
Alw26I	GTCTC	4 cut(s)	149, 215, 1041, 2460
AlwI	GGATC	4 cut(s)	560, 1295, 1448, 1649
AlwNI	CAGNNNCTG	3 cut(s)	1395, 1949, 2553
Aor13HI	TCCGGA	1 cut(s)	1456
AoxI	GGCC	7 cut(s)	327, 394, 668, 1321, 1769, 1946, 2421
ApeKI	GCWGC	9 cut(s)	79, 410, 461, 656, 1163, 1166, 1891, 2032, 2143
ApoI	RAATTY	3 cut(s)	775, 2049, 2068
AsiGI	ACCGGT	1 cut(s)	1329
Asp700I	GAANNNNTTC	2 cut(s)	62, 2400
AspA2I	CCTAGG	1 cut(s)	1228
AspLEI	GCGC	1 cut(s)	637
AspS9I	GGNCC	4 cut(s)	43, 394, 1946, 2498
AsuHPI	GGTGA	2 cut(s)	950, 2573
AvaII	GGWCC	2 cut(s)	43, 2498
AvrII	CCTAGG	1 cut(s)	1228
AxyI	CCTNAGG	2 cut(s)	401, 1938
BaeGI	GKGCMC	1 cut(s)	361
BbsI	GAAGAC	1 cut(s)	566
BbvI	GCAGC	9 cut(s)	91, 397, 448, 643, 1150, 1153, 1903, 2019, 2155
BccI	CCATC	7 cut(s)	14, 338, 524, 1075, 1150, 2489, 2578
BceAI	ACGGC	2 cut(s)	648, 2594
BcgI	CGANNNNNNTGC	2 cut(s)	1880, 1914
BciT130I	CCWGG	1 cut(s)	2479
BciVI	GTATCC	1 cut(s)	2233
BcoDI	GTCTC	4 cut(s)	149, 215, 1041, 2460
BcuI	ACTAGT	1 cut(s)	500
BfaI	CTAG	7 cut(s)	90, 473, 501, 761, 1229, 1895, 2730
BfmI	CTRYAG	1 cut(s)	1578
BfoI	RGCGCY	1 cut(s)	638
BfrI	CTTAAG	1 cut(s)	947
BfuAI	ACCTGC	1 cut(s)	2538
BfuI	GTATCC	1 cut(s)	2233
BglII	AGATCT	1 cut(s)	490
BlnI	CCTAGG	1 cut(s)	1228
Bme1390I	CCNGG	1 cut(s)	2479
Bme18I	GGWCC	2 cut(s)	43, 2498
BmeRI	GACNNNNNGTC	2 cut(s)	730, 2473
BmgT120I	GGNCC	4 cut(s)	43, 394, 1946, 2498
BmiI	GGNNCC	3 cut(s)	44, 45, 2157
BmrFI	CCNGG	1 cut(s)	2479
BmrI	ACTGGG	2 cut(s)	41, 1405
BmsI	GCATC	9 cut(s)	193, 229, 609, 898, 1150, 1237, 1264, 1585, 2681
BmuI	ACTGGG	2 cut(s)	41, 1405
BpiI	GAAGAC	1 cut(s)	566
BpmI	CTGGAG	3 cut(s)	1666, 1947, 2540
BpuEI	CTTGAG	1 cut(s)	510
Bsa29I	ATCGAT	2 cut(s)	852, 2675
BsaAI	YACGTR	1 cut(s)	2165
BsaBI	GATNNNNATC	3 cut(s)	852, 1483, 1709
BsaI	GGTCTC	1 cut(s)	2460
BsaJI	CCNNGG	3 cut(s)	595, 1228, 2512
BsaWI	WCCGGW	2 cut(s)	1329, 1456
Bsc4I	CCNNNNNNNGG	7 cut(s)	131, 132, 403, 406, 1943, 2255, 2518
Bse118I	RCCGGY	2 cut(s)	1329, 1767
Bse1I	ACTGG	7 cut(s)	47, 96, 899, 1400, 1732, 1819, 1857
Bse21I	CCTNAGG	2 cut(s)	401, 1938
Bse3DI	GCAATG	2 cut(s)	1292, 1347
Bse8I	GATNNNNATC	3 cut(s)	852, 1483, 1709
BseAI	TCCGGA	1 cut(s)	1456
BseBI	CCWGG	1 cut(s)	2479
BseCI	ATCGAT	2 cut(s)	852, 2675
BseDI	CCNNGG	3 cut(s)	595, 1228, 2512
BseGI	GGATG	3 cut(s)	25, 1165, 2460
BseJI	GATNNNNATC	3 cut(s)	852, 1483, 1709
BseLI	CCNNNNNNNGG	7 cut(s)	131, 132, 403, 406, 1943, 2255, 2518
BseMI	GCAATG	2 cut(s)	1292, 1347
BseMII	CTCAG	6 cut(s)	357, 405, 1929, 2070, 2365, 2441
BseNI	ACTGG	7 cut(s)	47, 96, 899, 1400, 1732, 1819, 1857
BseRI	GAGGAG	1 cut(s)	1521
BseSI	GKGCMC	1 cut(s)	361
BseXI	GCAGC	9 cut(s)	91, 397, 448, 643, 1150, 1153, 1903, 2019, 2155
BseYI	CCCAGC	1 cut(s)	2146
BsgI	GTGCAG	3 cut(s)	1026, 1758, 2435
BshFI	GGCC	7 cut(s)	329, 396, 670, 1323, 1771, 1948, 2423
BshTI	ACCGGT	1 cut(s)	1329
BshVI	ATCGAT	2 cut(s)	852, 2675
BsiSI	CCGG	3 cut(s)	1330, 1457, 1768
BslFI	GGGAC	5 cut(s)	56, 1406, 1449, 1454, 1631
BslI	CCNNNNNNNGG	7 cut(s)	131, 132, 403, 406, 1943, 2255, 2518
BsmAI	GTCTC	4 cut(s)	149, 215, 1041, 2460
BsmBI	CGTCTC	1 cut(s)	149
BsmFI	GGGAC	5 cut(s)	56, 1406, 1449, 1454, 1631
BsmI	GAATGC	4 cut(s)	355, 442, 473, 1916
BsnI	GGCC	7 cut(s)	329, 396, 670, 1323, 1771, 1948, 2423
Bso31I	GGTCTC	1 cut(s)	2460
Bsp1286I	GDGCHC	1 cut(s)	361
Bsp13I	TCCGGA	1 cut(s)	1456
Bsp1407I	TGTACA	2 cut(s)	381, 915
BspACI	CCGC	2 cut(s)	397, 459
BspANI	GGCC	7 cut(s)	329, 396, 670, 1323, 1771, 1948, 2423
BspCNI	CTCAG	6 cut(s)	356, 406, 1930, 2069, 2364, 2442
BspDI	ATCGAT	2 cut(s)	852, 2675
BspEI	TCCGGA	1 cut(s)	1456
BspHI	TCATGA	1 cut(s)	1354
BspLI	GGNNCC	3 cut(s)	44, 45, 2157
BspMAI	CTGCAG	1 cut(s)	1582
BspMI	ACCTGC	1 cut(s)	2538
BspPI	GGATC	4 cut(s)	560, 1295, 1448, 1649
BspQI	GCTCTTC	1 cut(s)	2610
BspTI	CTTAAG	1 cut(s)	947
BspTNI	GGTCTC	1 cut(s)	2460
BsrDI	GCAATG	2 cut(s)	1292, 1347
BsrFI	RCCGGY	2 cut(s)	1329, 1767
BsrGI	TGTACA	2 cut(s)	381, 915
BsrI	ACTGG	7 cut(s)	47, 96, 899, 1400, 1732, 1819, 1857
BssAI	RCCGGY	2 cut(s)	1329, 1767
BssECI	CCNNGG	3 cut(s)	595, 1228, 2512
BssT1I	CCWWGG	3 cut(s)	595, 1228, 2512
Bst2UI	CCWGG	1 cut(s)	2479
Bst4CI	ACNGT	6 cut(s)	74, 226, 317, 1113, 1144, 2359
Bst6I	CTCTTC	4 cut(s)	111, 131, 2610, 2731
BstAFI	CTTAAG	1 cut(s)	947
BstAPI	GCANNNNNTGC	1 cut(s)	2553
BstAUI	TGTACA	2 cut(s)	381, 915
BstBAI	YACGTR	1 cut(s)	2165
BstEII	GGTNACC	2 cut(s)	1799, 2605
BstENI	CCTNNNNNAGG	2 cut(s)	404, 1941
BstF5I	GGATG	3 cut(s)	25, 1165, 2460
BstH2I	RGCGCY	1 cut(s)	638
BstHHI	GCGC	1 cut(s)	637
BstMAI	GTCTC	4 cut(s)	149, 215, 1041, 2460
BstMWI	GCNNNNNNNGC	7 cut(s)	437, 699, 1193, 1274, 1535, 1888, 2553
BstNI	CCWGG	1 cut(s)	2479
BstNSI	RCATGY	6 cut(s)	357, 1767, 1886, 2274, 2327, 2542
BstPI	GGTNACC	2 cut(s)	1799, 2605
BstSCI	CCNGG	1 cut(s)	2477
BstSFI	CTRYAG	1 cut(s)	1578
BstSLI	GKGCMC	1 cut(s)	361
BstV1I	GCAGC	9 cut(s)	91, 397, 448, 643, 1150, 1153, 1903, 2019, 2155
BstV2I	GAAGAC	1 cut(s)	566
BstX2I	RGATCY	5 cut(s)	490, 565, 1300, 1453, 1641
BstXI	CCANNNNNNTGG	2 cut(s)	250, 337
BstYI	RGATCY	5 cut(s)	490, 565, 1300, 1453, 1641
Bsu15I	ATCGAT	2 cut(s)	852, 2675
Bsu36I	CCTNAGG	2 cut(s)	401, 1938
BsuI	GTATCC	1 cut(s)	2233
BsuRI	GGCC	7 cut(s)	329, 396, 670, 1323, 1771, 1948, 2423
BsuTUI	ATCGAT	2 cut(s)	852, 2675
BtsCI	GGATG	3 cut(s)	25, 1165, 2460
BtsI	GCAGTG	1 cut(s)	1071
BtsIMutI	CAGTG	5 cut(s)	103, 1071, 1140, 1850, 2364
BveI	ACCTGC	1 cut(s)	2538
CaiI	CAGNNNCTG	3 cut(s)	1395, 1949, 2553
CciI	TCATGA	1 cut(s)	1354
CfoI	GCGC	1 cut(s)	637
Cfr10I	RCCGGY	2 cut(s)	1329, 1767
Cfr13I	GGNCC	4 cut(s)	43, 394, 1946, 2498
ClaI	ATCGAT	2 cut(s)	852, 2675
Csp6I	GTAC	5 cut(s)	93, 382, 916, 2321, 2551
CspAI	ACCGGT	1 cut(s)	1329
CspCI	CAANNNNNGTGG	2 cut(s)	700, 735
CviQI	GTAC	5 cut(s)	93, 382, 916, 2321, 2551
DraI	TTTAAA	1 cut(s)	507
DraIII	CACNNNGTG	1 cut(s)	2165
DriI	GACNNNNNGTC	2 cut(s)	730, 2473
Eam1104I	CTCTTC	4 cut(s)	111, 131, 2610, 2731
Eam1105I	GACNNNNNGTC	2 cut(s)	730, 2473
EarI	CTCTTC	4 cut(s)	111, 131, 2610, 2731
Eco130I	CCWWGG	3 cut(s)	595, 1228, 2512
Eco31I	GGTCTC	1 cut(s)	2460
Eco32I	GATATC	2 cut(s)	850, 1711
Eco47I	GGWCC	2 cut(s)	43, 2498
Eco57I	CTGAAG	2 cut(s)	702, 2634
Eco81I	CCTNAGG	2 cut(s)	401, 1938
Eco91I	GGTNACC	2 cut(s)	1799, 2605
EcoNI	CCTNNNNNAGG	2 cut(s)	404, 1941
EcoO109I	RGGNCCY	2 cut(s)	43, 1946
EcoO65I	GGTNACC	2 cut(s)	1799, 2605
EcoRI	GAATTC	1 cut(s)	2049
EcoRII	CCWGG	1 cut(s)	2477
EcoRV	GATATC	2 cut(s)	850, 1711
EcoT14I	CCWWGG	3 cut(s)	595, 1228, 2512
EcoT22I	ATGCAT	2 cut(s)	355, 1279
ErhI	CCWWGG	3 cut(s)	595, 1228, 2512
Esp3I	CGTCTC	1 cut(s)	149
FalI	AAGNNNNNCTT	4 cut(s)	292, 324, 1518, 1550
FaqI	GGGAC	5 cut(s)	56, 1406, 1449, 1454, 1631
FauI	CCCGC	1 cut(s)	466
FauNDI	CATATG	3 cut(s)	1207, 1279, 2205
FblI	GTMKAC	3 cut(s)	606, 1137, 1402
FokI	GGATG	3 cut(s)	32, 1172, 2467
FspBI	CTAG	7 cut(s)	90, 473, 501, 761, 1229, 1895, 2730
GlaI	GCGC	1 cut(s)	636
GsaI	CCCAGC	1 cut(s)	2150
GsuI	CTGGAG	3 cut(s)	1666, 1947, 2540
HaeII	RGCGCY	1 cut(s)	638
HaeIII	GGCC	7 cut(s)	329, 396, 670, 1323, 1771, 1948, 2423
HapII	CCGG	3 cut(s)	1330, 1457, 1768
HhaI	GCGC	1 cut(s)	637
Hin6I	GCGC	1 cut(s)	635
HinP1I	GCGC	1 cut(s)	635
HindIII	AAGCTT	2 cut(s)	509, 552
HpaII	CCGG	3 cut(s)	1330, 1457, 1768
HphI	GGTGA	2 cut(s)	950, 2573
Hpy166II	GTNNAC	4 cut(s)	607, 1138, 1403, 2508
Hpy188I	TCNGA	8 cut(s)	202, 346, 435, 613, 1080, 2428, 2451, 2750
Hpy8I	GTNNAC	4 cut(s)	607, 1138, 1403, 2508
HpyCH4III	ACNGT	6 cut(s)	74, 226, 317, 1113, 1144, 2359
HpyCH4IV	ACGT	3 cut(s)	1405, 1462, 2164
HpyF10VI	GCNNNNNNNGC	7 cut(s)	437, 699, 1193, 1274, 1535, 1888, 2553
HpySE526I	ACGT	3 cut(s)	1405, 1462, 2164
HspAI	GCGC	1 cut(s)	635
KflI	GGGWCCC	1 cut(s)	43
Kpn2I	TCCGGA	1 cut(s)	1456
KroI	GCCGGC	1 cut(s)	1767
KroNI	GCCGGC	1 cut(s)	1769
LguI	GCTCTTC	1 cut(s)	2610
LmnI	GCTCC	3 cut(s)	583, 1534, 2140
Lsp1109I	GCAGC	9 cut(s)	91, 397, 448, 643, 1150, 1153, 1903, 2019, 2155
LweI	GCATC	9 cut(s)	193, 229, 609, 898, 1150, 1237, 1264, 1585, 2681
MaeI	CTAG	7 cut(s)	90, 473, 501, 761, 1229, 1895, 2730
MaeII	ACGT	3 cut(s)	1405, 1462, 2164
MaeIII	GTNAC	5 cut(s)	746, 1786, 1799, 2302, 2605
MfeI	CAATTG	2 cut(s)	963, 1827
MflI	RGATCY	5 cut(s)	490, 565, 1300, 1453, 1641
MhlI	GDGCHC	1 cut(s)	361
MlyI	GAGTC	5 cut(s)	335, 425, 740, 2467, 2531
MmeI	TCCRAC	5 cut(s)	210, 318, 2133, 2709, 2729
Mph1103I	ATGCAT	2 cut(s)	355, 1279
MroI	TCCGGA	1 cut(s)	1456
MroNI	GCCGGC	1 cut(s)	1767
MroXI	GAANNNNTTC	2 cut(s)	62, 2400
MseI	TTAA	7 cut(s)	485, 506, 834, 948, 1293, 2001, 2694
MslI	CAYNNNNRTG	1 cut(s)	1548
MspA1I	CMGCKG	2 cut(s)	461, 2146
MspCI	CTTAAG	1 cut(s)	947
MspI	CCGG	3 cut(s)	1330, 1457, 1768
MspR9I	CCNGG	1 cut(s)	2479
MunI	CAATTG	2 cut(s)	963, 1827
Mva1269I	GAATGC	4 cut(s)	355, 442, 473, 1916
MvaI	CCWGG	1 cut(s)	2479
MwoI	GCNNNNNNNGC	7 cut(s)	437, 699, 1193, 1274, 1535, 1888, 2553
NaeI	GCCGGC	1 cut(s)	1769
NdeI	CATATG	3 cut(s)	1207, 1279, 2205
NgoMIV	GCCGGC	1 cut(s)	1767
NlaIV	GGNNCC	3 cut(s)	44, 45, 2157
NsiI	ATGCAT	2 cut(s)	355, 1279
NspI	RCATGY	6 cut(s)	357, 1767, 1886, 2274, 2327, 2542
PaeI	GCATGC	1 cut(s)	1767
PagI	TCATGA	1 cut(s)	1354
PciI	ACATGT	2 cut(s)	2270, 2538
PciSI	GCTCTTC	1 cut(s)	2610
PctI	GAATGC	4 cut(s)	355, 442, 473, 1916
PdiI	GCCGGC	1 cut(s)	1769
PdmI	GAANNNNTTC	2 cut(s)	62, 2400
PfeI	GAWTC	8 cut(s)	63, 1307, 1839, 1847, 1859, 1868, 2276, 2657
PflFI	GACNNNGTC	1 cut(s)	1622
PinAI	ACCGGT	1 cut(s)	1329
PleI	GAGTC	5 cut(s)	335, 424, 739, 2466, 2530
PpsI	GAGTC	5 cut(s)	335, 424, 739, 2466, 2530
Ppu21I	YACGTR	1 cut(s)	2165
PpuMI	RGGWCCY	1 cut(s)	43
PscI	ACATGT	2 cut(s)	2270, 2538
PsiI	TTATAA	2 cut(s)	591, 891
Psp5II	RGGWCCY	1 cut(s)	43
Psp6I	CCWGG	1 cut(s)	2477
PspEI	GGTNACC	2 cut(s)	1799, 2605
PspFI	CCCAGC	1 cut(s)	2146
PspGI	CCWGG	1 cut(s)	2477
PspN4I	GGNNCC	3 cut(s)	44, 45, 2157
PspPI	GGNCC	4 cut(s)	43, 394, 1946, 2498
PspPPI	RGGWCCY	1 cut(s)	43
PstI	CTGCAG	1 cut(s)	1582
PstNI	CAGNNNCTG	3 cut(s)	1395, 1949, 2553
PsuI	RGATCY	5 cut(s)	490, 565, 1300, 1453, 1641
PsyI	GACNNNGTC	1 cut(s)	1622
PvuII	CAGCTG	1 cut(s)	2146
RsaI	GTAC	5 cut(s)	94, 383, 917, 2322, 2552
RsaNI	GTAC	5 cut(s)	93, 382, 916, 2321, 2551
RseI	CAYNNNNRTG	1 cut(s)	1548
SapI	GCTCTTC	1 cut(s)	2610
SaqAI	TTAA	7 cut(s)	485, 506, 834, 948, 1293, 2001, 2694
Sau96I	GGNCC	4 cut(s)	43, 394, 1946, 2498
SchI	GAGTC	5 cut(s)	335, 425, 740, 2467, 2531
ScrFI	CCNGG	1 cut(s)	2479
SduI	GDGCHC	1 cut(s)	361
SfaNI	GCATC	9 cut(s)	193, 229, 609, 898, 1150, 1237, 1264, 1585, 2681
SfcI	CTRYAG	1 cut(s)	1578
SinI	GGWCC	2 cut(s)	43, 2498
SmiMI	CAYNNNNRTG	1 cut(s)	1548
SmlI	CTYRAG	2 cut(s)	525, 947
SmoI	CTYRAG	2 cut(s)	525, 947
SpeI	ACTAGT	1 cut(s)	500
SphI	GCATGC	1 cut(s)	1767
SsiI	CCGC	2 cut(s)	397, 459
SspI	AATATT	1 cut(s)	1258
SspMI	CTAG	7 cut(s)	90, 473, 501, 761, 1229, 1895, 2730
StyD4I	CCNGG	1 cut(s)	2477
StyI	CCWWGG	3 cut(s)	595, 1228, 2512
TaaI	ACNGT	6 cut(s)	74, 226, 317, 1113, 1144, 2359
TaiI	ACGT	3 cut(s)	1408, 1465, 2167
TaqI	TCGA	3 cut(s)	852, 1900, 2675
TatI	WGTACW	3 cut(s)	381, 915, 2320
TauI	GCSGC	1 cut(s)	399
TfiI	GAWTC	8 cut(s)	63, 1307, 1839, 1847, 1859, 1868, 2276, 2657
Tru1I	TTAA	7 cut(s)	485, 506, 834, 948, 1293, 2001, 2694
Tru9I	TTAA	7 cut(s)	485, 506, 834, 948, 1293, 2001, 2694
TscAI	CASTG	5 cut(s)	103, 1071, 1147, 1857, 2364
TseI	GCWGC	9 cut(s)	79, 410, 461, 656, 1163, 1166, 1891, 2032, 2143
TspDTI	ATGAA	9 cut(s)	302, 438, 564, 990, 1343, 1358, 1371, 1560, 1683
TspGWI	ACGGA	3 cut(s)	26, 338, 2703
TspRI	CASTG	5 cut(s)	103, 1071, 1147, 1857, 2364
Tth111I	GACNNNGTC	1 cut(s)	1622
Vha464I	CTTAAG	1 cut(s)	947
VpaK11BI	GGWCC	2 cut(s)	43, 2498
XagI	CCTNNNNNAGG	2 cut(s)	404, 1941
XapI	RAATTY	3 cut(s)	775, 2049, 2068
XceI	RCATGY	6 cut(s)	357, 1767, 1886, 2274, 2327, 2542
XmaJI	CCTAGG	1 cut(s)	1228
XmiI	GTMKAC	3 cut(s)	606, 1137, 1402
XmnI	GAANNNNTTC	2 cut(s)	62, 2400
XspI	CTAG	7 cut(s)	90, 473, 501, 761, 1229, 1895, 2730
Zsp2I	ATGCAT	2 cut(s)	355, 1279

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.