Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0008031

Physical Location & Seq

Genomic Coordinates Forward (+)

2796124 .. 2805929

Gene Structure 9806 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1031300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2754 bp

ATGGCATGGACGGAGAAAGATGTTGGTAATGTGAGAAATGGAGAGCCGGCTGGAGAGAATGGATTCTTGAATGATTCGCAGCCTTCGCCCAGTACCAGTGGTTCTATGGTTGCCGGAACTCCGGCAGGGAAGATGTTTGAAGTGAAAGATGCTCTTTCTTATGCCAACATTCTTCGTTCGAGGAACAAGTTTGCTGATGCTCTGAGTCTTTACGGGACTGTGTTGGAGAAAGATCCTGGAAATGTGGAAGCTCATATTGGAAAAGGAATATGCTTGCAGATGAAGAATATGGGAAGGCTCGCTTTTGACAGTTTCGCAGAAGCCATCAGGTTGGACCCACAGAATGCATGCGCTCACACGCATTGTGGAATTTTGTACAAAGAAGAGGGCCGTCTAAGGGAGGCTGCTGAGTCTTATCAAAAAGCTCTGAAAGCAGACCAATTCTACAAACCCGCTGCGGAATGCTTGGCCATTGTGTTAACTGATCTTGGAACCAGCTTAAAGCTTGCCGGAAATACTCAAGAGGGACTGCAGAAGTATTATGAAGCTTTGAAGACAGATCCGCACTATGCTCCTGCATATTATAACCTTGGCGTTGTCTACTCTGAAATGATGCAATTTGACATGGCCCTTACTTGCTACGAGAAGGCTGCATTGGAGAGGCCGATGTATGCTGAAGCATATTGCAACATGGGTGTCATTTACAAAAATCGTGGTGATTTGGAATCTGCTATTGCTTGCTACGAGAGGTGTCTTGCTGTTTCGCCAAACTTTGAGATTGCAAAGAATAATATGGCAATAGCATTAACAGATTTAGGAACAAAGGTTAAATTGGAAGGAGACATTGATCAAGGCATATCCTATTACAAAAAGGCTTTGTATTATAACTGGCATTATGCCGATGCTATGTACAATCTTGGGGTTGCTTATGGTGAAATGCTTAAGTTTGACATGGCAATTGTGTTCTATGAACTTGCTTTCCACTTCAATCCCCATTGTGCAGAAGCATGCAATAATTTAGGAGTAATATACAAAGATCGAGACAACCTTGATAAAGCAGTAGAGTGTTATCAGATGGCTTTGTCAATCAAACCAAACTTCTCACAATCGTTGAACAATCTTGGTGTTGTCTACACTGTCCAGGGTAAAATGGATGCTGCTGCAAACATGATTGACAAAGCTATAGTTGCAAATCCCACATATGCAGAAGCATACAACAACTTAGGGGTTCTCTACCGCGATGCTGGAAATATTACTCTCGCCATTGGTGCATATGAGGAATGCCTCAAAATAGATCCCGATTCCCGAAATGCAGGCCAGAATCGATTGCTTGCGATGAACTACATAAATGAAGGACATGATGATAAACTTTTTGTCGCTCACAGGGATTGGGGTAGGCGCTTTATGAGGTTATATCCACAGTACACTTCATGGGACAATCCAAAAGATCCAGAACGACCCCTCATCATTGGATATGTGTCTCCTGATTATTTTACTCATTCTGTTTCATATTTTATAGAAGCTCCGCTTGCCCATCATGAATACACAAAGTACAAGGTGGTTGTGTATTCTGCAGTAGTGAAGGCAGATGCCAAGACTATTAGGTTTCGGGAGAAAGTCTTGAAAAAGGGTGGGATTTGGAGAGATATATATGGGATTGATGAAAAGAAGGTTGCAAGCATAATTAGAGAAGATAAGGTTGATATCTTGGTGGAACTTACTGGCCATACTGCAAACAATAAATTGGGAACGATGGCATGCAGGCCCTCACCTGTTCAGGTGACCTGGATTGGCTACCCAAACACAACTGGTTTGCCGGCGATTGATTATAGAATCACAGATTCTCTGGCAGATCCACCTGATTCAAAGCAGACGCATGTTGAGGACTTAGTTCGATTACCCGATTGCTTCCTGTGTTATACACCTTCCCCTGAGGCTGGGCCTGTTATGCCCACTCCTGCTCTTTCCAATGGCTTTGTTACTTTTGGAAGCTTTAATAATCTGGCAAAGATCACTCCAAAAGTGCTACAAGTTTGGGCAAGAATTCTTTCTGCAATTCCGAATTCTCGCCTTGTGGTGAAGTGTAAGCCTTTTAGTTGTGATAGTGTGAGGGAGAGATTTCTTTCAACATTGGAGCAGCTGGGGTTGGAACCACTACGTGTTGATCTTCTGCCTCTAATTTTACTCAACTACGATCATATGCAAGCCTATTCTCTCATGGACATTAGTTTGGATACTTTTCCATATGCTGGAACAACCACAACATGTGAATCCTTATATATGGGAGTCCCGTGTGTTACTATGGCTGGTTCAGTACATGCGCACAACGTTGGTGTTAGTATTCTCAGCAAAGTAGGGTTGGGAAATCTGATTGCTAAAAATGAGGATGAATATGTCCAGTTGGCAGTGCAGCTGGCCTCTGACGTAACAGCGCTTTCAAATTTGAGAATGGGTCTCCGGGACCTCATGGCCAGGTCGCCTGTCTGTGACGGACCAAAGTTTACCTCTGGGTTGGAGTCAGCATACAGGAGCATGTGGCACAGGTACTGCAAAGGTGATGTGCCATCACAACGGCAAATGGAAATGCTACAAGAGGAAGTAATTGCTGAAGACCCTGCTGCTGGAATCTCTGATCCCACAAGTATTACAACACCAAGAGAAGGGCCTCCTGGATCTATTAAGACTAATGGATTTGGTCCTGTTCCAACCCCTGCTCTTAACCCCTCCGCTTGTGAAGAAAATGGAGGAGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0007154 GO:0007165 GO:0008150 GO:0008194 GO:0008375 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009739 GO:0009740 GO:0009755 GO:0009937 GO:0009938 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010476 GO:0010646 GO:0010648 GO:0016262 GO:0016740 GO:0016757 GO:0016758 GO:0019222 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0032870 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071368 GO:0071370 GO:0071396 GO:0071495 GO:0140096 GO:1901700 GO:1901701 GO:2000377

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_transf_41 TPR_1 TPR_10 TPR_16 TPR_7 TPR_8

Protein Analysis

918

Amino Acids

101.48

Weight (kDa)

5.58

Isoelectric Point (pI)

38.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_1	PF00515	191 - 217	2.7e-07	Tetratricopeptide repeat
TPR_1	PF00515	225 - 258	8.3e-10	Tetratricopeptide repeat
TPR_2	PF07719	225 - 257	8.5e-07	Tetratricopeptide repeat
TPR_8	PF13181	225 - 257	6.9e-08	Tetratricopeptide repeat
TPR_12	PF13424	225 - 293	1.4e-06	Tetratricopeptide repeat
TPR_1	PF00515	334 - 367	9.2e-10	Tetratricopeptide repeat
TPR_2	PF07719	334 - 366	3.6e-06	Tetratricopeptide repeat
TPR_11	PF13414	343 - 381	2.9e-06	TPR repeat
TPR_12	PF13424	369 - 432	2.4e-06	Tetratricopeptide repeat
TPR_16	PF13432	380 - 435	7.4e-07	Tetratricopeptide repeat
TPR_1	PF00515	402 - 434	5.6e-08	Tetratricopeptide repeat
TPR_2	PF07719	402 - 434	2.7e-06	Tetratricopeptide repeat
TPR_8	PF13181	402 - 434	6.6e-06	Tetratricopeptide repeat
Glyco_transf_41	PF13844	482 - 638	3.9e-23	Glycosyl transferase family 41
Glyco_transf_41	PF13844	655 - 836	1.8e-24	Glycosyl transferase family 41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008031)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11540 AT3G11540
fragaria_vesca	FvH4_6g19470 FvH4_6g19470 FvH4_6g19470 FvH4_6g19470
malus_domestica	MD12G1030800.v1.1 MD14G1031300.v1.1
prunus_persica	Prupe.7G101600_v2.0.a1 Prupe.7G101600_v2.0.a1
pyrus_communis	pycom12g02880 pycom14g02820
rosa_chinensis	RchiOBHm_Chr3g0474801
rosa_laevigata	RLG00000023896
rosa_multiflora	Rmu_sc0004848.1_g000005
rosa_roxburghii	Rroxscaffold_6G00406850
rosa_rugosa	Rorug03G0143700 Rorug03G0143800 Rorug03G0143900
rosa_samantha	Rh3AG193600 Rh3BG223400 Rh3CG218300 Rh3DG219400
rosa_wichuraiana	Rw3G017640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	585, 885
Acc16I	TGCGCA	1 cut(s)	2322
AccB7I	CCANNNNNTGG	1 cut(s)	2249
AccI	GTMKAC	2 cut(s)	600, 1131
AccII	CGCG	1 cut(s)	1239
AciI	CCGC	6 cut(s)	453, 458, 563, 1237, 1526, 2727
AclI	AACGTT	1 cut(s)	2328
AclWI	GGATC	7 cut(s)	227, 554, 1289, 1442, 1847, 2627, 2680
AcoI	YGGCCR	3 cut(s)	468, 1723, 2469
AcsI	RAATTY	4 cut(s)	369, 2043, 2062, 2440
AcuI	CTGAAG	2 cut(s)	696, 2628
AdeI	CACNNNGTG	1 cut(s)	2159
AfaI	GTAC	7 cut(s)	94, 377, 911, 1424, 1553, 2316, 2546
AfeI	AGCGCT	1 cut(s)	2433
AfiI	CCNNNNNNNGG	5 cut(s)	397, 1937, 2249, 2621, 2660
AflII	CTTAAG	1 cut(s)	941
AflIII	ACRYGT	2 cut(s)	2158, 2264
AgsI	TTSAA	9 cut(s)	70, 140, 553, 988, 1114, 1624, 1866, 2127, 2439
AjnI	CCWGG	5 cut(s)	235, 1140, 1784, 2471, 2668
AjuI	GAANNNNNNNTTGG	4 cut(s)	240, 272, 638, 670
AloI	GAACNNNNNNTCC	2 cut(s)	963, 995
Alw26I	GTCTC	4 cut(s)	834, 1035, 1485, 2459
AlwI	GGATC	7 cut(s)	227, 554, 1289, 1442, 1847, 2627, 2680
AlwNI	CAGNNNCTG	1 cut(s)	2547
Aor51HI	AGCGCT	1 cut(s)	2433
ApeKI	GCWGC	9 cut(s)	79, 404, 455, 650, 1157, 1160, 2137, 2410, 2618
ApoI	RAATTY	4 cut(s)	369, 2043, 2062, 2440
Asp700I	GAANNNNTTC	2 cut(s)	62, 2047
AspLEI	GCGC	4 cut(s)	353, 1401, 2323, 2434
AspS9I	GGNCC	9 cut(s)	334, 388, 628, 1764, 1940, 2461, 2492, 2663, 2696
AsuC2I	CCSGG	1 cut(s)	2459
AsuHPI	GGTGA	6 cut(s)	728, 944, 1761, 1792, 2089, 2567
AvaII	GGWCC	4 cut(s)	334, 2461, 2492, 2696
AxyI	CCTNAGG	1 cut(s)	1932
BalI	TGGCCA	3 cut(s)	470, 1725, 2471
BbsI	GAAGAC	2 cut(s)	560, 2616
BbvI	GCAGC	9 cut(s)	91, 391, 442, 637, 1144, 1147, 2149, 2422, 2605
BccI	CCATC	5 cut(s)	332, 1069, 1542, 1747, 2572
BceAI	ACGGC	2 cut(s)	375, 2588
BcgI	CGANNNNNNTGC	2 cut(s)	632, 666
BciT130I	CCWGG	5 cut(s)	237, 1142, 1786, 2473, 2670
BciVI	GTATCC	1 cut(s)	2227
BclI	TGATCA	1 cut(s)	847
BcnI	CCSGG	1 cut(s)	2459
BcoDI	GTCTC	4 cut(s)	834, 1035, 1485, 2459
BfmI	CTRYAG	3 cut(s)	530, 1182, 1572
BfoI	RGCGCY	2 cut(s)	1402, 2435
BfrI	CTTAAG	1 cut(s)	941
BfuI	GTATCC	1 cut(s)	2227
BisI	GCNGC	9 cut(s)	80, 405, 456, 651, 1158, 1161, 2138, 2411, 2619
BlsI	GCNGC	9 cut(s)	81, 406, 457, 652, 1159, 1162, 2139, 2412, 2620
Bme1390I	CCNGG	6 cut(s)	237, 1142, 1786, 2459, 2473, 2670
Bme18I	GGWCC	4 cut(s)	334, 2461, 2492, 2696
BmgT120I	GGNCC	9 cut(s)	334, 388, 628, 1764, 1940, 2461, 2492, 2663, 2696
BmiI	GGNNCC	4 cut(s)	336, 493, 2151, 2462
BmrFI	CCNGG	6 cut(s)	237, 1142, 1786, 2459, 2473, 2670
BmrI	ACTGGG	1 cut(s)	84
BmsI	GCATC	7 cut(s)	139, 187, 603, 892, 1144, 1231, 1579
BmuI	ACTGGG	1 cut(s)	84
BpiI	GAAGAC	2 cut(s)	560, 2616
BpmI	CTGGAG	1 cut(s)	72
BpuEI	CTTGAG	1 cut(s)	504
BpuMI	CCSGG	1 cut(s)	2459
Bsa29I	ATCGAT	1 cut(s)	1324
BsaAI	YACGTR	1 cut(s)	2159
BsaI	GGTCTC	1 cut(s)	2459
BsaJI	CCNNGG	2 cut(s)	589, 1141
Bsc4I	CCNNNNNNNGG	5 cut(s)	397, 1937, 2249, 2621, 2660
Bse118I	RCCGGY	2 cut(s)	46, 1816
Bse1I	ACTGG	6 cut(s)	90, 96, 893, 1726, 1813, 2398
Bse21I	CCTNAGG	1 cut(s)	1932
BseBI	CCWGG	5 cut(s)	237, 1142, 1786, 2473, 2670
BseCI	ATCGAT	1 cut(s)	1324
BseDI	CCNNGG	2 cut(s)	589, 1141
BseGI	GGATG	2 cut(s)	1159, 2392
BseLI	CCNNNNNNNGG	5 cut(s)	397, 1937, 2249, 2621, 2660
BseMII	CTCAG	4 cut(s)	194, 399, 1923, 2359
BseNI	ACTGG	6 cut(s)	90, 96, 893, 1726, 1813, 2398
BseXI	GCAGC	9 cut(s)	91, 391, 442, 637, 1144, 1147, 2149, 2422, 2605
BseYI	CCCAGC	2 cut(s)	1937, 2140
BsgI	GTGCAG	2 cut(s)	1020, 2429
Bsh1236I	CGCG	1 cut(s)	1239
BshVI	ATCGAT	1 cut(s)	1324
BsiSI	CCGG	6 cut(s)	47, 114, 122, 510, 1817, 2458
BslFI	GGGAC	5 cut(s)	229, 540, 1448, 2273, 2474
BslI	CCNNNNNNNGG	5 cut(s)	397, 1937, 2249, 2621, 2660
BsmAI	GTCTC	4 cut(s)	834, 1035, 1485, 2459
BsmFI	GGGAC	5 cut(s)	229, 540, 1448, 2273, 2474
BsmI	GAATGC	3 cut(s)	349, 467, 1286
Bso31I	GGTCTC	1 cut(s)	2459
Bsp1407I	TGTACA	2 cut(s)	375, 909
BspACI	CCGC	6 cut(s)	453, 458, 563, 1237, 1526, 2727
BspCNI	CTCAG	4 cut(s)	195, 400, 1924, 2358
BspDI	ATCGAT	1 cut(s)	1324
BspFNI	CGCG	1 cut(s)	1239
BspHI	TCATGA	1 cut(s)	1537
BspLI	GGNNCC	4 cut(s)	336, 493, 2151, 2462
BspMAI	CTGCAG	2 cut(s)	534, 1576
BspPI	GGATC	7 cut(s)	227, 554, 1289, 1442, 1847, 2627, 2680
BspTI	CTTAAG	1 cut(s)	941
BspTNI	GGTCTC	1 cut(s)	2459
BsrFI	RCCGGY	2 cut(s)	46, 1816
BsrGI	TGTACA	2 cut(s)	375, 909
BsrI	ACTGG	6 cut(s)	90, 96, 893, 1726, 1813, 2398
BssAI	RCCGGY	2 cut(s)	46, 1816
BssECI	CCNNGG	2 cut(s)	589, 1141
BssT1I	CCWWGG	1 cut(s)	589
Bst2UI	CCWGG	5 cut(s)	237, 1142, 1786, 2473, 2670
Bst4CI	ACNGT	4 cut(s)	220, 311, 1138, 1422
Bst6I	CTCTTC	1 cut(s)	378
BstAFI	CTTAAG	1 cut(s)	941
BstAUI	TGTACA	2 cut(s)	375, 909
BstBAI	YACGTR	1 cut(s)	2159
BstDEI	CTNAG	7 cut(s)	203, 395, 408, 1222, 1888, 1932, 2345
BstEII	GGTNACC	1 cut(s)	1780
BstF5I	GGATG	2 cut(s)	1159, 2392
BstFNI	CGCG	1 cut(s)	1239
BstH2I	RGCGCY	2 cut(s)	1402, 2435
BstHHI	GCGC	4 cut(s)	353, 1401, 2323, 2434
BstMAI	GTCTC	4 cut(s)	834, 1035, 1485, 2459
BstMWI	GCNNNNNNNGC	6 cut(s)	85, 431, 1187, 1268, 1529, 1948
BstNI	CCWGG	5 cut(s)	237, 1142, 1786, 2473, 2670
BstNSI	RCATGY	7 cut(s)	351, 1011, 1761, 1880, 2268, 2321, 2536
BstPI	GGTNACC	1 cut(s)	1780
BstSCI	CCNGG	6 cut(s)	235, 1140, 1784, 2457, 2471, 2668
BstSFI	CTRYAG	3 cut(s)	530, 1182, 1572
BstUI	CGCG	1 cut(s)	1239
BstV1I	GCAGC	9 cut(s)	91, 391, 442, 637, 1144, 1147, 2149, 2422, 2605
BstV2I	GAAGAC	2 cut(s)	560, 2616
BstX2I	RGATCY	6 cut(s)	232, 559, 1294, 1447, 1852, 2672
BstXI	CCANNNNNNTGG	1 cut(s)	331
BstYI	RGATCY	6 cut(s)	232, 559, 1294, 1447, 1852, 2672
Bsu15I	ATCGAT	1 cut(s)	1324
Bsu36I	CCTNAGG	1 cut(s)	1932
BsuI	GTATCC	1 cut(s)	2227
BsuTUI	ATCGAT	1 cut(s)	1324
BtgZI	GCGATG	2 cut(s)	1254, 1349
BtsCI	GGATG	2 cut(s)	1159, 2392
BtsI	GCAGTG	1 cut(s)	2412
BtsIMutI	CAGTG	3 cut(s)	103, 1134, 2412
CaiI	CAGNNNCTG	1 cut(s)	2547
CciI	TCATGA	1 cut(s)	1537
CfoI	GCGC	4 cut(s)	353, 1401, 2323, 2434
Cfr10I	RCCGGY	2 cut(s)	46, 1816
Cfr13I	GGNCC	9 cut(s)	334, 388, 628, 1764, 1940, 2461, 2492, 2663, 2696
ClaI	ATCGAT	1 cut(s)	1324
CseI	GACGC	1 cut(s)	1882
Csp6I	GTAC	7 cut(s)	93, 376, 910, 1423, 1552, 2315, 2545
CspCI	CAANNNNNGTGG	2 cut(s)	694, 729
CviQI	GTAC	7 cut(s)	93, 376, 910, 1423, 1552, 2315, 2545
DdeI	CTNAG	7 cut(s)	203, 395, 408, 1222, 1888, 1932, 2345
DraIII	CACNNNGTG	1 cut(s)	2159
EaeI	YGGCCR	3 cut(s)	468, 1723, 2469
Eam1104I	CTCTTC	1 cut(s)	378
EarI	CTCTTC	1 cut(s)	378
Eco130I	CCWWGG	1 cut(s)	589
Eco31I	GGTCTC	1 cut(s)	2459
Eco32I	GATATC	1 cut(s)	1705
Eco47I	GGWCC	4 cut(s)	334, 2461, 2492, 2696
Eco47III	AGCGCT	1 cut(s)	2433
Eco57I	CTGAAG	2 cut(s)	696, 2628
Eco81I	CCTNAGG	1 cut(s)	1932
Eco91I	GGTNACC	1 cut(s)	1780
EcoO109I	RGGNCCY	3 cut(s)	1764, 2461, 2663
EcoO65I	GGTNACC	1 cut(s)	1780
EcoRI	GAATTC	2 cut(s)	2043, 2062
EcoRII	CCWGG	5 cut(s)	235, 1140, 1784, 2471, 2668
EcoRV	GATATC	1 cut(s)	1705
EcoT14I	CCWWGG	1 cut(s)	589
EcoT22I	ATGCAT	1 cut(s)	349
ErhI	CCWWGG	1 cut(s)	589
FalI	AAGNNNNNCTT	6 cut(s)	138, 170, 286, 318, 1512, 1544
FaqI	GGGAC	5 cut(s)	229, 540, 1448, 2273, 2474
FauI	CCCGC	1 cut(s)	460
FauNDI	CATATG	4 cut(s)	1201, 1273, 2199, 2245
FbaI	TGATCA	1 cut(s)	847
FblI	GTMKAC	2 cut(s)	600, 1131
Fnu4HI	GCNGC	9 cut(s)	80, 405, 456, 651, 1158, 1161, 2138, 2411, 2619
FokI	GGATG	2 cut(s)	1166, 2399
Fsp4HI	GCNGC	9 cut(s)	80, 405, 456, 651, 1158, 1161, 2138, 2411, 2619
FspAI	RTGCGCAY	1 cut(s)	2322
FspI	TGCGCA	1 cut(s)	2322
GlaI	GCGC	4 cut(s)	352, 1400, 2322, 2433
GluI	GCNGC	9 cut(s)	80, 405, 456, 651, 1158, 1161, 2138, 2411, 2619
GsaI	CCCAGC	2 cut(s)	1941, 2144
GsuI	CTGGAG	1 cut(s)	72
HaeII	RGCGCY	2 cut(s)	1402, 2435
HapII	CCGG	6 cut(s)	47, 114, 122, 510, 1817, 2458
HgaI	GACGC	1 cut(s)	1882
HhaI	GCGC	4 cut(s)	353, 1401, 2323, 2434
Hin6I	GCGC	4 cut(s)	351, 1399, 2321, 2432
HinP1I	GCGC	4 cut(s)	351, 1399, 2321, 2432
HincII	GTYRAC	1 cut(s)	480
HindII	GTYRAC	1 cut(s)	480
HindIII	AAGCTT	3 cut(s)	503, 546, 1990
HpaI	GTTAAC	1 cut(s)	480
HpaII	CCGG	6 cut(s)	47, 114, 122, 510, 1817, 2458
HphI	GGTGA	6 cut(s)	728, 944, 1761, 1792, 2089, 2567
Hpy166II	GTNNAC	5 cut(s)	480, 601, 1132, 1425, 2502
Hpy188I	TCNGA	9 cut(s)	204, 429, 607, 1074, 2061, 2370, 2422, 2632, 2753
Hpy8I	GTNNAC	5 cut(s)	480, 601, 1132, 1425, 2502
HpyAV	CCTTC	9 cut(s)	93, 288, 640, 830, 1346, 1576, 1663, 1935, 2654
HpyCH4III	ACNGT	4 cut(s)	220, 311, 1138, 1422
HpyCH4IV	ACGT	3 cut(s)	2158, 2328, 2424
HpyF10VI	GCNNNNNNNGC	6 cut(s)	85, 431, 1187, 1268, 1529, 1948
HpyF3I	CTNAG	7 cut(s)	203, 395, 408, 1222, 1888, 1932, 2345
HpySE526I	ACGT	3 cut(s)	2158, 2328, 2424
HspAI	GCGC	4 cut(s)	351, 1399, 2321, 2432
KroI	GCCGGC	2 cut(s)	46, 1816
KroNI	GCCGGC	2 cut(s)	48, 1818
Ksp22I	TGATCA	1 cut(s)	847
KspAI	GTTAAC	1 cut(s)	480
LmnI	GCTCC	4 cut(s)	577, 1528, 2134, 2529
Lsp1109I	GCAGC	9 cut(s)	91, 391, 442, 637, 1144, 1147, 2149, 2422, 2605
LweI	GCATC	7 cut(s)	139, 187, 603, 892, 1144, 1231, 1579
MaeII	ACGT	3 cut(s)	2158, 2328, 2424
MaeIII	GTNAC	5 cut(s)	1780, 1978, 2296, 2425, 2486
MboII	GAAGA	9 cut(s)	142, 164, 295, 395, 565, 1703, 2159, 2621, 2747
MfeI	CAATTG	1 cut(s)	957
MflI	RGATCY	6 cut(s)	232, 559, 1294, 1447, 1852, 2672
MlsI	TGGCCA	3 cut(s)	470, 1725, 2471
MluNI	TGGCCA	3 cut(s)	470, 1725, 2471
MlyI	GAGTC	4 cut(s)	214, 419, 2295, 2525
MmeI	TCCRAC	5 cut(s)	204, 312, 2127, 2493, 2729
Mox20I	TGGCCA	3 cut(s)	470, 1725, 2471
Mph1103I	ATGCAT	1 cut(s)	349
MroNI	GCCGGC	2 cut(s)	46, 1816
MroXI	GAANNNNTTC	2 cut(s)	62, 2047
MscI	TGGCCA	3 cut(s)	470, 1725, 2471
MseI	TTAA	8 cut(s)	479, 500, 806, 828, 942, 1995, 2679, 2718
Msp20I	TGGCCA	3 cut(s)	470, 1725, 2471
MspA1I	CMGCKG	3 cut(s)	455, 2140, 2413
MspCI	CTTAAG	1 cut(s)	941
MspI	CCGG	6 cut(s)	47, 114, 122, 510, 1817, 2458
MspR9I	CCNGG	6 cut(s)	237, 1142, 1786, 2459, 2473, 2670
MunI	CAATTG	1 cut(s)	957
Mva1269I	GAATGC	3 cut(s)	349, 467, 1286
MvaI	CCWGG	5 cut(s)	237, 1142, 1786, 2473, 2670
MvnI	CGCG	1 cut(s)	1239
MwoI	GCNNNNNNNGC	6 cut(s)	85, 431, 1187, 1268, 1529, 1948
NaeI	GCCGGC	2 cut(s)	48, 1818
NciI	CCSGG	1 cut(s)	2459
NdeI	CATATG	4 cut(s)	1201, 1273, 2199, 2245
NgoMIV	GCCGGC	2 cut(s)	46, 1816
NlaIV	GGNNCC	4 cut(s)	336, 493, 2151, 2462
NmuCI	GTSAC	2 cut(s)	1780, 2486
NsbI	TGCGCA	1 cut(s)	2322
NsiI	ATGCAT	1 cut(s)	349
NspI	RCATGY	7 cut(s)	351, 1011, 1761, 1880, 2268, 2321, 2536
PaeI	GCATGC	3 cut(s)	351, 1011, 1761
PagI	TCATGA	1 cut(s)	1537
PciI	ACATGT	1 cut(s)	2264
PctI	GAATGC	3 cut(s)	349, 467, 1286
PdiI	GCCGGC	2 cut(s)	48, 1818
PdmI	GAANNNNTTC	2 cut(s)	62, 2047
PflMI	CCANNNNNTGG	1 cut(s)	2249
PfoI	TCCNGGA	3 cut(s)	235, 2457, 2668
PkrI	GCNGC	9 cut(s)	81, 406, 457, 652, 1159, 1162, 2139, 2412, 2620
PleI	GAGTC	4 cut(s)	213, 418, 2294, 2524
PpsI	GAGTC	4 cut(s)	213, 418, 2294, 2524
Ppu21I	YACGTR	1 cut(s)	2159
PpuMI	RGGWCCY	1 cut(s)	2461
PscI	ACATGT	1 cut(s)	2264
PsiI	TTATAA	2 cut(s)	585, 885
Psp1406I	AACGTT	1 cut(s)	2328
Psp5II	RGGWCCY	1 cut(s)	2461
Psp6I	CCWGG	5 cut(s)	235, 1140, 1784, 2471, 2668
PspEI	GGTNACC	1 cut(s)	1780
PspFI	CCCAGC	2 cut(s)	1937, 2140
PspGI	CCWGG	5 cut(s)	235, 1140, 1784, 2471, 2668
PspN4I	GGNNCC	4 cut(s)	336, 493, 2151, 2462
PspPI	GGNCC	9 cut(s)	334, 388, 628, 1764, 1940, 2461, 2492, 2663, 2696
PspPPI	RGGWCCY	1 cut(s)	2461
PsrI	GAACNNNNNNTAC	2 cut(s)	85, 117
PstI	CTGCAG	2 cut(s)	534, 1576
PstNI	CAGNNNCTG	1 cut(s)	2547
PsuI	RGATCY	6 cut(s)	232, 559, 1294, 1447, 1852, 2672
PvuII	CAGCTG	2 cut(s)	2140, 2413
RsaI	GTAC	7 cut(s)	94, 377, 911, 1424, 1553, 2316, 2546
RsaNI	GTAC	7 cut(s)	93, 376, 910, 1423, 1552, 2315, 2545
SaqAI	TTAA	8 cut(s)	479, 500, 806, 828, 942, 1995, 2679, 2718
SatI	GCNGC	9 cut(s)	80, 405, 456, 651, 1158, 1161, 2138, 2411, 2619
Sau96I	GGNCC	9 cut(s)	334, 388, 628, 1764, 1940, 2461, 2492, 2663, 2696
SchI	GAGTC	4 cut(s)	214, 419, 2295, 2525
ScrFI	CCNGG	6 cut(s)	237, 1142, 1786, 2459, 2473, 2670
SfaNI	GCATC	7 cut(s)	139, 187, 603, 892, 1144, 1231, 1579
SfcI	CTRYAG	3 cut(s)	530, 1182, 1572
SinI	GGWCC	4 cut(s)	334, 2461, 2492, 2696
SmlI	CTYRAG	2 cut(s)	519, 941
SmoI	CTYRAG	2 cut(s)	519, 941
SphI	GCATGC	3 cut(s)	351, 1011, 1761
SsiI	CCGC	6 cut(s)	453, 458, 563, 1237, 1526, 2727
SspI	AATATT	1 cut(s)	1252
StyD4I	CCNGG	6 cut(s)	235, 1140, 1784, 2457, 2471, 2668
StyI	CCWWGG	1 cut(s)	589
TaaI	ACNGT	4 cut(s)	220, 311, 1138, 1422
TaiI	ACGT	3 cut(s)	2161, 2331, 2427
TaqI	TCGA	4 cut(s)	179, 1039, 1324, 1894
TatI	WGTACW	5 cut(s)	375, 909, 1422, 1551, 2314
Tru1I	TTAA	8 cut(s)	479, 500, 806, 828, 942, 1995, 2679, 2718
Tru9I	TTAA	8 cut(s)	479, 500, 806, 828, 942, 1995, 2679, 2718
TscAI	CASTG	3 cut(s)	103, 1141, 2412
TseFI	GTSAC	2 cut(s)	1780, 2486
TseI	GCWGC	9 cut(s)	79, 404, 455, 650, 1157, 1160, 2137, 2410, 2618
Tsp45I	GTSAC	2 cut(s)	1780, 2486
TspGWI	ACGGA	2 cut(s)	26, 2505
TspRI	CASTG	3 cut(s)	103, 1141, 2412
Van91I	CCANNNNNTGG	1 cut(s)	2249
Vha464I	CTTAAG	1 cut(s)	941
VpaK11BI	GGWCC	4 cut(s)	334, 2461, 2492, 2696
XapI	RAATTY	4 cut(s)	369, 2043, 2062, 2440
XceI	RCATGY	7 cut(s)	351, 1011, 1761, 1880, 2268, 2321, 2536
XcmI	CCANNNNNNNNNTGG	1 cut(s)	103
XmiI	GTMKAC	2 cut(s)	600, 1131
XmnI	GAANNNNTTC	2 cut(s)	62, 2047
Zsp2I	ATGCAT	1 cut(s)	349

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.