MD12G1079200.v1.1

transcription regulatory region sequence-specific DNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016404
Physical Location & Seq
Reverse (-)
9641548 .. 9642234
687 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1079200.v1.1.491

Sequence Viewer

Length: 687 bp
ATGCCCAGGAGCAAAGTGAAACTTGCTTGGATAGTGAACGATAATGCTCGGAAAGCCACCTCCAAAAGAAGTAGAGAAAGCTTTTTTAAAAAAATGCGTGAAATAACCACCTTATGTGATGTGGGTGGCTTCACCATTGTCTATGACCCGGCCACTGGTGAGCCTAGTACATGGCCTGAGCCCCCATTACTCCAACAACTAATTGCAAGGTACCAAAGCATACCATTTCCCGCGCGGTTGGCGAAGATGACGGACCAGGAAACTTACCTCAAGGAGAAGGTAACCAAACTTAAAGATCGAATCAACAGGATTCAAAGAGAGAACTTTGATGTGGAGGTGAATGATATCATGCACCAGATCACTCAAAATGGTAAGCCATTTCATACATTTGAAGATAGAGAGCTAACCAATTTGCTTTTGTTTACGGAGGAGAAGATGAAAGAGATTCAGAAACGGATCAAAACTATGGAGCCAGAAGTTAACGTTCCACCATCTGATGGTGGAGGAGAATTTAACATGAGAGTTCCAGAGCATGGAGAGGACCGTCGAGAGTATCTCTTTAAGTATTTGGAATGGGCAAAAAGGAAGCTAGCAATGGAGAAGGCTGCTGGAGCTGTACAAAGCAGTTTCCATAACTGTTCGGAAGTGGCAACGGTGGAGATGATGTTGGGATTTCGTAATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_43

Protein Analysis

229

Amino Acids

26.56

Weight (kDa)

8.95

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 11 - 58 2.6e-07 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 210
AccB1I GGYRCC 1 cut(s) 210
AccB7I CCANNNNNTGG 2 cut(s) 497, 533
AccII CGCG 2 cut(s) 233, 235
AciI CCGC 2 cut(s) 231, 235
AclI AACGTT 1 cut(s) 483
AclWI GGATC 1 cut(s) 464
AcoI YGGCCR 1 cut(s) 150
AcsI RAATTY 1 cut(s) 509
AfaI GTAC 3 cut(s) 169, 212, 618
AfiI CCNNNNNNNGG 3 cut(s) 155, 497, 533
AgsI TTSAA 2 cut(s) 314, 392
AjnI CCWGG 2 cut(s) 5, 255
AluBI AGCT 4 cut(s) 81, 403, 589, 614
AluI AGCT 4 cut(s) 81, 403, 589, 614
AlwI GGATC 1 cut(s) 464
AoxI GGCC 2 cut(s) 150, 173
ApeKI GCWGC 1 cut(s) 605
ApoI RAATTY 1 cut(s) 509
Asp718I GGTACC 1 cut(s) 210
AspLEI GCGC 1 cut(s) 235
AspS9I GGNCC 2 cut(s) 253, 541
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 3 cut(s) 124, 170, 349
AsuNHI GCTAGC 1 cut(s) 589
AvaII GGWCC 2 cut(s) 253, 541
BanI GGYRCC 1 cut(s) 210
BanII GRGCYC 1 cut(s) 183
BbvI GCAGC 1 cut(s) 592
BccI CCATC 2 cut(s) 491, 499
BciT130I CCWGG 2 cut(s) 7, 257
BcnI CCSGG 1 cut(s) 149
BfaI CTAG 2 cut(s) 165, 590
BisI GCNGC 1 cut(s) 606
BlsI GCNGC 1 cut(s) 607
Bme1390I CCNGG 3 cut(s) 7, 149, 257
Bme18I GGWCC 2 cut(s) 253, 541
BmgT120I GGNCC 2 cut(s) 253, 541
BmiI GGNNCC 2 cut(s) 212, 471
BmrFI CCNGG 3 cut(s) 7, 149, 257
BmtI GCTAGC 1 cut(s) 593
BplI GAGNNNNNCTC 2 cut(s) 540, 572
BpmI CTGGAG 1 cut(s) 630
Bpu10I CCTNAGC 1 cut(s) 177
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 5
BsaXI ACNNNNNCTCC 2 cut(s) 650, 680
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 497, 533
Bse1I ACTGG 1 cut(s) 160
Bse3DI GCAATG 1 cut(s) 600
BseBI CCWGG 2 cut(s) 7, 257
BseDI CCNNGG 1 cut(s) 5
BseLI CCNNNNNNNGG 3 cut(s) 155, 497, 533
BseMI GCAATG 1 cut(s) 600
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 160
BseRI GAGGAG 2 cut(s) 443, 519
BseXI GCAGC 1 cut(s) 592
Bsh1236I CGCG 2 cut(s) 233, 235
BshFI GGCC 2 cut(s) 152, 175
BshNI GGYRCC 1 cut(s) 210
BsiSI CCGG 1 cut(s) 149
BslI CCNNNNNNNGG 3 cut(s) 155, 497, 533
BsnI GGCC 2 cut(s) 152, 175
Bsp1286I GDGCHC 1 cut(s) 183
Bsp1407I TGTACA 1 cut(s) 616
Bsp143I GATC 3 cut(s) 295, 357, 456
BspACI CCGC 2 cut(s) 231, 235
BspANI GGCC 2 cut(s) 152, 175
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 2 cut(s) 233, 235
BspLI GGNNCC 2 cut(s) 212, 471
BspOI GCTAGC 1 cut(s) 593
BspPI GGATC 1 cut(s) 464
BspT107I GGYRCC 1 cut(s) 210
BsrDI GCAATG 1 cut(s) 600
BsrGI TGTACA 1 cut(s) 616
BsrI ACTGG 1 cut(s) 160
BssECI CCNNGG 1 cut(s) 5
BssMI GATC 3 cut(s) 295, 357, 456
Bst2UI CCWGG 2 cut(s) 7, 257
Bst4CI ACNGT 3 cut(s) 545, 638, 655
BstAUI TGTACA 1 cut(s) 616
BstC8I GCNNGC 1 cut(s) 591
BstDEI CTNAG 1 cut(s) 177
BstEII GGTNACC 1 cut(s) 280
BstFNI CGCG 2 cut(s) 233, 235
BstHHI GCGC 1 cut(s) 235
BstKTI GATC 3 cut(s) 298, 360, 459
BstMBI GATC 3 cut(s) 295, 357, 456
BstMWI GCNNNNNNNGC 3 cut(s) 53, 239, 611
BstNI CCWGG 2 cut(s) 7, 257
BstPI GGTNACC 1 cut(s) 280
BstSCI CCNGG 3 cut(s) 5, 147, 255
BstUI CGCG 2 cut(s) 233, 235
BstV1I GCAGC 1 cut(s) 592
BsuRI GGCC 2 cut(s) 152, 175
BtsIMutI CAGTG 1 cut(s) 153
Cac8I GCNNGC 1 cut(s) 591
CfoI GCGC 1 cut(s) 235
Cfr13I GGNCC 2 cut(s) 253, 541
Csp6I GTAC 3 cut(s) 168, 211, 617
CviAII CATG 4 cut(s) 171, 349, 517, 533
CviQI GTAC 3 cut(s) 168, 211, 617
DdeI CTNAG 1 cut(s) 177
DpnI GATC 3 cut(s) 297, 359, 458
DpnII GATC 3 cut(s) 295, 357, 456
DraI TTTAAA 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 183
Eco32I GATATC 1 cut(s) 346
Eco47I GGWCC 2 cut(s) 253, 541
Eco91I GGTNACC 1 cut(s) 280
EcoO65I GGTNACC 1 cut(s) 280
EcoRII CCWGG 2 cut(s) 5, 255
EcoRV GATATC 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 183
FaeI CATG 4 cut(s) 174, 352, 520, 536
FalI AAGNNNNNCTT 1 cut(s) 38
FatI CATG 4 cut(s) 170, 348, 516, 532
FauI CCCGC 1 cut(s) 238
Fnu4HI GCNGC 1 cut(s) 606
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 1 cut(s) 606
FspBI CTAG 2 cut(s) 165, 590
GlaI GCGC 1 cut(s) 234
GluI GCNGC 1 cut(s) 606
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 2 cut(s) 152, 175
HapII CCGG 1 cut(s) 149
HhaI GCGC 1 cut(s) 235
Hin1II CATG 4 cut(s) 174, 352, 520, 536
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HindIII AAGCTT 1 cut(s) 79
HinfI GANTC 3 cut(s) 300, 310, 445
HpaI GTTAAC 1 cut(s) 481
HpaII CCGG 1 cut(s) 149
HphI GGTGA 3 cut(s) 124, 170, 349
Hpy166II GTNNAC 3 cut(s) 37, 423, 481
Hpy188I TCNGA 4 cut(s) 51, 450, 496, 643
Hpy188III TCNNGA 2 cut(s) 527, 548
Hpy8I GTNNAC 3 cut(s) 37, 423, 481
Hpy99I CGWCG 1 cut(s) 549
HpyAV CCTTC 2 cut(s) 271, 595
HpyCH4III ACNGT 3 cut(s) 545, 638, 655
HpyCH4IV ACGT 1 cut(s) 483
HpyCH4V TGCA 2 cut(s) 206, 352
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 239, 611
HpyF3I CTNAG 1 cut(s) 177
HpySE526I ACGT 1 cut(s) 483
Hsp92II CATG 4 cut(s) 174, 352, 520, 536
HspAI GCGC 1 cut(s) 233
KpnI GGTACC 1 cut(s) 214
KspAI GTTAAC 1 cut(s) 481
Kzo9I GATC 3 cut(s) 295, 357, 456
LmnI GCTCC 3 cut(s) 9, 469, 611
Lsp1109I GCAGC 1 cut(s) 592
MaeI CTAG 2 cut(s) 165, 590
MaeII ACGT 1 cut(s) 483
MaeIII GTNAC 1 cut(s) 280
MalI GATC 3 cut(s) 297, 359, 458
MboI GATC 3 cut(s) 295, 357, 456
MboII GAAGA 3 cut(s) 256, 404, 445
MhlI GDGCHC 1 cut(s) 183
MluCI AATT 3 cut(s) 201, 409, 509
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 6 cut(s) 70, 278, 328, 421, 497, 532
MseI TTAA 5 cut(s) 87, 291, 480, 513, 561
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 3 cut(s) 7, 149, 257
MvaI CCWGG 2 cut(s) 7, 257
MvnI CGCG 2 cut(s) 233, 235
MwoI GCNNNNNNNGC 3 cut(s) 53, 239, 611
NciI CCSGG 1 cut(s) 149
NdeII GATC 3 cut(s) 295, 357, 456
NheI GCTAGC 1 cut(s) 589
NlaIII CATG 4 cut(s) 174, 352, 520, 536
NlaIV GGNNCC 2 cut(s) 212, 471
PfeI GAWTC 3 cut(s) 300, 310, 445
PflMI CCANNNNNTGG 2 cut(s) 497, 533
PkrI GCNGC 1 cut(s) 607
Psp1406I AACGTT 1 cut(s) 483
Psp6I CCWGG 2 cut(s) 5, 255
PspEI GGTNACC 1 cut(s) 280
PspGI CCWGG 2 cut(s) 5, 255
PspN4I GGNNCC 2 cut(s) 212, 471
PspPI GGNCC 2 cut(s) 253, 541
RsaI GTAC 3 cut(s) 169, 212, 618
RsaNI GTAC 3 cut(s) 168, 211, 617
SaqAI TTAA 5 cut(s) 87, 291, 480, 513, 561
SatI GCNGC 1 cut(s) 606
Sau3AI GATC 3 cut(s) 295, 357, 456
Sau96I GGNCC 2 cut(s) 253, 541
ScrFI CCNGG 3 cut(s) 7, 149, 257
SduI GDGCHC 1 cut(s) 183
SinI GGWCC 2 cut(s) 253, 541
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 3 cut(s) 201, 409, 509
SsiI CCGC 2 cut(s) 231, 235
SspMI CTAG 2 cut(s) 165, 590
StyD4I CCNGG 3 cut(s) 5, 147, 255
TaaI ACNGT 3 cut(s) 545, 638, 655
TaiI ACGT 1 cut(s) 486
TaqI TCGA 2 cut(s) 298, 547
TasI AATT 3 cut(s) 201, 409, 509
TatI WGTACW 2 cut(s) 167, 616
TfiI GAWTC 3 cut(s) 300, 310, 445
Tru1I TTAA 5 cut(s) 87, 291, 480, 513, 561
Tru9I TTAA 5 cut(s) 87, 291, 480, 513, 561
TscAI CASTG 1 cut(s) 160
TseI GCWGC 1 cut(s) 605
TspDTI ATGAA 2 cut(s) 371, 452
TspGWI ACGGA 3 cut(s) 266, 440, 469
TspRI CASTG 1 cut(s) 160
Van91I CCANNNNNTGG 2 cut(s) 497, 533
VpaK11BI GGWCC 2 cut(s) 253, 541
XapI RAATTY 1 cut(s) 509
XspI CTAG 2 cut(s) 165, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.