MD14G1041400.v1.1
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016404
Physical Location & Seq
Reverse (-)
3896276 .. 3897046
771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1041400.v1.1.491

Sequence Viewer

Length: 771 bp
ATGGGTAAGAAGGTGAAACTTCAATGGGTGGAAAATGATAGAGCTAGGAAAGAATGCTTTAGAAAGAGGAAGGCAAATTTGTTAAGGAAAATGGATGAAATAACCACCTTATGCCAAGTGAATGCCTTTGCCATCGTATACGGGCAAGAAAACAATGATCCAACGTTGTGGCCTTCTCACCCGGAGGTGGAAGAACTGATTAAAAGGTTCTTCAGCATACCGAATGTGGAGCGGTACAAGAAGTCGTTGAACCAAGAGACGTATCTTCTGGAGAGGGCGGTCAGATTCCGAGAAAGAATAAAAAAGATCAACAAACAGATTCGGGAAATGAAAGTGAACAAACTCGTGCATCAAGTTCACGAAGGTAAATCACTGAATGAATTTGAAAGAAGTGACATGACTGATTTGGTTTCCTTTTGTGAGGAAAAAATGTACGGGATCAAGAAATTGGTTGCGCATTTGGAGAAAGAGGACCCGAATCGCGAACAAGGCACCTCCAACTATGAGAAAGGGAAGGAGATTGAGAACGCTGCTGGTAATATTGCTGCCAATGGGATTGAGAACTCTGGAGATGCCCTAGGGCTGGGGCTGATGTCTCACAGTGGAGCTGGAAAAGAGGTAGGGCAACCTGATGTGAGCCATGAGATGGTAAAGCAAAGTGAGAATATAACTGGAAAAAAAGGTAAGCGCATTAGACTGATGGGTGTTGCTATCGAAGGCAGCGTTGCAGATGATGATGATGCAGAGGATCCGCCACAGCATGGTAACTAG

Protein Analysis

257

Amino Acids

29.16

Weight (kDa)

7.71

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 51 2.6e-11 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 456
AccB1I GGYRCC 1 cut(s) 491
AccB7I CCANNNNNTGG 2 cut(s) 646, 761
AccBSI CCGCTC 1 cut(s) 232
AccI GTMKAC 1 cut(s) 138
AccII CGCG 1 cut(s) 483
AciI CCGC 3 cut(s) 232, 278, 752
AclI AACGTT 1 cut(s) 164
AclWI GGATC 4 cut(s) 152, 446, 743, 756
AcsI RAATTY 2 cut(s) 76, 380
AcuI CTGAAG 1 cut(s) 196
AfaI GTAC 2 cut(s) 236, 434
AfiI CCNNNNNNNGG 4 cut(s) 187, 583, 646, 761
AgsI TTSAA 3 cut(s) 23, 250, 386
AluBI AGCT 2 cut(s) 44, 608
AluI AGCT 2 cut(s) 44, 608
Alw26I GTCTC 2 cut(s) 251, 600
AlwI GGATC 4 cut(s) 152, 446, 743, 756
AoxI GGCC 1 cut(s) 170
ApeKI GCWGC 3 cut(s) 530, 545, 720
ApoI RAATTY 2 cut(s) 76, 380
AspA2I CCTAGG 1 cut(s) 577
AspLEI GCGC 2 cut(s) 457, 690
AspS9I GGNCC 1 cut(s) 472
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 2 cut(s) 25, 170
AvaII GGWCC 1 cut(s) 472
AvrII CCTAGG 1 cut(s) 577
BamHI GGATCC 1 cut(s) 748
BanI GGYRCC 1 cut(s) 491
BauI CACGAG 1 cut(s) 344
BbvI GCAGC 3 cut(s) 517, 532, 732
BccI CCATC 3 cut(s) 140, 640, 694
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 2 cut(s) 251, 600
BfaI CTAG 3 cut(s) 45, 578, 769
BisI GCNGC 3 cut(s) 531, 546, 721
BlnI CCTAGG 1 cut(s) 577
BlsI GCNGC 3 cut(s) 532, 547, 722
Bme1390I CCNGG 1 cut(s) 182
Bme18I GGWCC 1 cut(s) 472
BmgT120I GGNCC 1 cut(s) 472
BmiI GGNNCC 3 cut(s) 474, 493, 750
BmrFI CCNGG 1 cut(s) 182
BmsI GCATC 3 cut(s) 358, 562, 730
BpmI CTGGAG 2 cut(s) 290, 588
BpuMI CCSGG 1 cut(s) 182
BsaJI CCNNGG 1 cut(s) 577
Bsc4I CCNNNNNNNGG 4 cut(s) 187, 583, 646, 761
Bse1I ACTGG 1 cut(s) 676
BseDI CCNNGG 1 cut(s) 577
BseGI GGATG 1 cut(s) 100
BseLI CCNNNNNNNGG 4 cut(s) 187, 583, 646, 761
BseNI ACTGG 1 cut(s) 676
BseXI GCAGC 3 cut(s) 517, 532, 732
BseYI CCCAGC 1 cut(s) 583
Bsh1236I CGCG 1 cut(s) 483
BshFI GGCC 1 cut(s) 172
BshNI GGYRCC 1 cut(s) 491
BsiSI CCGG 1 cut(s) 182
BslI CCNNNNNNNGG 4 cut(s) 187, 583, 646, 761
BsmAI GTCTC 2 cut(s) 251, 600
BsmBI CGTCTC 1 cut(s) 251
BsmI GAATGC 2 cut(s) 59, 127
BsnI GGCC 1 cut(s) 172
Bsp143I GATC 4 cut(s) 157, 306, 438, 748
Bsp68I TCGCGA 1 cut(s) 483
BspACI CCGC 3 cut(s) 232, 278, 752
BspANI GGCC 1 cut(s) 172
BspFNI CGCG 1 cut(s) 483
BspLI GGNNCC 3 cut(s) 474, 493, 750
BspPI GGATC 4 cut(s) 152, 446, 743, 756
BspT107I GGYRCC 1 cut(s) 491
BsrBI CCGCTC 1 cut(s) 232
BsrI ACTGG 1 cut(s) 676
BssECI CCNNGG 1 cut(s) 577
BssMI GATC 4 cut(s) 157, 306, 438, 748
BssNAI GTATAC 1 cut(s) 139
BssSI CACGAG 1 cut(s) 344
BssT1I CCWWGG 1 cut(s) 577
Bst1107I GTATAC 1 cut(s) 139
Bst2BI CACGAG 1 cut(s) 344
Bst4CI ACNGT 1 cut(s) 602
BstF5I GGATG 1 cut(s) 100
BstFNI CGCG 1 cut(s) 483
BstHHI GCGC 2 cut(s) 457, 690
BstKTI GATC 4 cut(s) 160, 309, 441, 751
BstMAI GTCTC 2 cut(s) 251, 600
BstMBI GATC 4 cut(s) 157, 306, 438, 748
BstMWI GCNNNNNNNGC 1 cut(s) 489
BstSCI CCNGG 1 cut(s) 180
BstUI CGCG 1 cut(s) 483
BstV1I GCAGC 3 cut(s) 517, 532, 732
BstX2I RGATCY 1 cut(s) 748
BstXI CCANNNNNNTGG 1 cut(s) 168
BstYI RGATCY 1 cut(s) 748
BstZ17I GTATAC 1 cut(s) 139
BsuRI GGCC 1 cut(s) 172
BtsCI GGATG 1 cut(s) 100
BtsIMutI CAGTG 2 cut(s) 371, 607
BtuMI TCGCGA 1 cut(s) 483
CfoI GCGC 2 cut(s) 457, 690
Cfr13I GGNCC 1 cut(s) 472
Csp6I GTAC 2 cut(s) 235, 433
CviAII CATG 3 cut(s) 397, 641, 761
CviJI RGCY 6 cut(s) 44, 172, 583, 589, 608, 639
CviKI_1 RGCY 6 cut(s) 44, 172, 583, 589, 608, 639
CviQI GTAC 2 cut(s) 235, 433
DpnI GATC 4 cut(s) 159, 308, 440, 750
DpnII GATC 4 cut(s) 157, 306, 438, 748
EciI GGCGGA 1 cut(s) 741
Eco130I CCWWGG 1 cut(s) 577
Eco47I GGWCC 1 cut(s) 472
Eco57I CTGAAG 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 472
EcoT14I CCWWGG 1 cut(s) 577
ErhI CCWWGG 1 cut(s) 577
Esp3I CGTCTC 1 cut(s) 251
FaeI CATG 3 cut(s) 400, 644, 764
FaiI YATR 8 cut(s) 112, 139, 218, 398, 504, 642, 668, 762
FatI CATG 3 cut(s) 396, 640, 760
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 3 cut(s) 531, 546, 721
FokI GGATG 1 cut(s) 107
Fsp4HI GCNGC 3 cut(s) 531, 546, 721
FspBI CTAG 3 cut(s) 45, 578, 769
FspI TGCGCA 1 cut(s) 456
GlaI GCGC 2 cut(s) 456, 689
GluI GCNGC 3 cut(s) 531, 546, 721
GsaI CCCAGC 1 cut(s) 587
GsuI CTGGAG 2 cut(s) 290, 588
HaeIII GGCC 1 cut(s) 172
HapII CCGG 1 cut(s) 182
HhaI GCGC 2 cut(s) 457, 690
Hin1II CATG 3 cut(s) 400, 644, 764
Hin6I GCGC 2 cut(s) 455, 688
HinP1I GCGC 2 cut(s) 455, 688
HinfI GANTC 3 cut(s) 285, 319, 478
HpaII CCGG 1 cut(s) 182
HphI GGTGA 2 cut(s) 25, 170
Hpy166II GTNNAC 3 cut(s) 139, 337, 358
Hpy188I TCNGA 2 cut(s) 284, 290
Hpy188III TCNNGA 6 cut(s) 269, 323, 359, 442, 482, 567
Hpy8I GTNNAC 3 cut(s) 139, 337, 358
HpyAV CCTTC 6 cut(s) 4, 64, 183, 356, 508, 710
HpyCH4III ACNGT 1 cut(s) 602
HpyCH4IV ACGT 2 cut(s) 164, 260
HpyCH4V TGCA 3 cut(s) 349, 728, 743
HpyF10VI GCNNNNNNNGC 1 cut(s) 489
HpySE526I ACGT 2 cut(s) 164, 260
Hsp92II CATG 3 cut(s) 400, 644, 764
HspAI GCGC 2 cut(s) 455, 688
Kzo9I GATC 4 cut(s) 157, 306, 438, 748
LmnI GCTCC 2 cut(s) 229, 605
LpnPI CCDG 8 cut(s) 195, 254, 519, 552, 569, 594, 642, 657
Lsp1109I GCAGC 3 cut(s) 517, 532, 732
LweI GCATC 3 cut(s) 358, 562, 730
MaeI CTAG 3 cut(s) 45, 578, 769
MaeII ACGT 2 cut(s) 164, 260
MaeIII GTNAC 2 cut(s) 392, 764
MalI GATC 4 cut(s) 159, 308, 440, 750
MbiI CCGCTC 1 cut(s) 232
MboI GATC 4 cut(s) 157, 306, 438, 748
MboII GAAGA 3 cut(s) 202, 203, 257
MflI RGATCY 1 cut(s) 748
MluCI AATT 3 cut(s) 76, 380, 446
MmeI TCCRAC 2 cut(s) 185, 522
MnlI CCTC 8 cut(s) 60, 178, 267, 415, 463, 505, 610, 739
MseI TTAA 2 cut(s) 83, 201
MspI CCGG 1 cut(s) 182
MspR9I CCNGG 1 cut(s) 182
Mva1269I GAATGC 2 cut(s) 59, 127
MvnI CGCG 1 cut(s) 483
MwoI GCNNNNNNNGC 1 cut(s) 489
NciI CCSGG 1 cut(s) 182
NdeII GATC 4 cut(s) 157, 306, 438, 748
NlaIII CATG 3 cut(s) 400, 644, 764
NlaIV GGNNCC 3 cut(s) 474, 493, 750
NmuCI GTSAC 1 cut(s) 392
NruI TCGCGA 1 cut(s) 483
NsbI TGCGCA 1 cut(s) 456
PcsI WCGNNNNNNNCGW 1 cut(s) 720
PctI GAATGC 2 cut(s) 59, 127
PfeI GAWTC 3 cut(s) 285, 319, 478
PflMI CCANNNNNTGG 2 cut(s) 646, 761
PkrI GCNGC 3 cut(s) 532, 547, 722
PpuMI RGGWCCY 1 cut(s) 472
Psp1406I AACGTT 1 cut(s) 164
Psp5II RGGWCCY 1 cut(s) 472
PspFI CCCAGC 1 cut(s) 583
PspN4I GGNNCC 3 cut(s) 474, 493, 750
PspPI GGNCC 1 cut(s) 472
PspPPI RGGWCCY 1 cut(s) 472
PsuI RGATCY 1 cut(s) 748
RruI TCGCGA 1 cut(s) 483
RsaI GTAC 2 cut(s) 236, 434
RsaNI GTAC 2 cut(s) 235, 433
SaqAI TTAA 2 cut(s) 83, 201
SatI GCNGC 3 cut(s) 531, 546, 721
Sau3AI GATC 4 cut(s) 157, 306, 438, 748
Sau96I GGNCC 1 cut(s) 472
ScrFI CCNGG 1 cut(s) 182
SfaNI GCATC 3 cut(s) 358, 562, 730
SinI GGWCC 1 cut(s) 472
Sse9I AATT 3 cut(s) 76, 380, 446
SsiI CCGC 3 cut(s) 232, 278, 752
SspI AATATT 1 cut(s) 541
SspMI CTAG 3 cut(s) 45, 578, 769
StyD4I CCNGG 1 cut(s) 180
StyI CCWWGG 1 cut(s) 577
TaaI ACNGT 1 cut(s) 602
TaiI ACGT 2 cut(s) 167, 263
TaqI TCGA 1 cut(s) 714
TasI AATT 3 cut(s) 76, 380, 446
TfiI GAWTC 3 cut(s) 285, 319, 478
Tru1I TTAA 2 cut(s) 83, 201
Tru9I TTAA 2 cut(s) 83, 201
TscAI CASTG 2 cut(s) 378, 607
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 3 cut(s) 530, 545, 720
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 3 cut(s) 111, 344, 393
TspRI CASTG 2 cut(s) 378, 607
Van91I CCANNNNNTGG 2 cut(s) 646, 761
VpaK11BI GGWCC 1 cut(s) 472
XapI RAATTY 2 cut(s) 76, 380
XmaJI CCTAGG 1 cut(s) 577
XmiI GTMKAC 1 cut(s) 138
XspI CTAG 3 cut(s) 45, 578, 769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.