Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006652

Physical Location & Seq

Genomic Coordinates Reverse (-)

10579144 .. 10584956

Gene Structure 5813 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1086300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1629 bp

ATGGAGGAGGAGAAACCTCTATCAACTTTACCGGAGCCATCTGTTGCAGCAGTCCCAATTGCTCCAGTCCGCGCTCCTCCCCTCAGACCGCCGCCGCCAGTAAATGGGGAAGCCAGGACGAGCACAAGTGACTCCGATGATTCCGACTCGGAGGAGCCTCCACGAGCTACCGGCGTTTCGGGTGGGGAGTACGAGATATCGGAAGAGAGCAGAGTGGTGAGAGAGCGGCAGGAGAAAGCAATGCAGGAACTGCTGATGAAGCGCCGTGCTTCTGCTCTTGCTGTTCCCACCAACGACATGGCCGTGAGGGCTCGTCTTCGGCGGCTCGGCGAACCCATTACTCTCTTCGGGGAGAGAGAGATGGAAAGGCGAGACAGGTTGAGGATGATCATGGCGAGGCTGGATTCGCAAGGGGAGTTGGAGAAGTTGTTGAAAGTTCATGAGGAGGAGGAGGCTGCAGCTTCTACTGCTGCAGTGGAGGATGGTGATGATCAAATTATTCAGTACCCTTTTTACACTGAAGGGTCCAAAGCTTTGTTGAATGCTAGGATTGACATTGCCAAGTACTCCATTAGGAGGGCGGCCTTGCGGCTCGAACGGGCTCGAAGAAAGAAGGACGACCCAGATGAGGATATGGATGCAGAGTTGGACTTGGTTTTGGAGCAGGCAAAAAGTTTGGAACTTGATTGCAGTGAGATTGGGGATGATAGGCCACTTTCGGGTTGTTCTTTCTCACATGATGGGCAACTGCTTGCCACTTGTGCTATAAGTGGAGTGGCCAAGTTGTGGAGCATGCCTGAAGCAAAGAAGGTTACCACTTTAAAGGGACACACAGAGCGACTGACTGATGTTCAGTTTTCTCCTGTGCATAACCTCTTAGCAACTGCCTCTGCTGACCGGACTGCAAGGTTGTGGAACACAGAAGGAACTCAGCTGTTGACATTTAAGGGCCATTTGGACCGTCTGGCACGAATTGCCTTCCATCCATCAGGTAAGTACTTGGGTACGACTAGCTTTGACCAGACTTGGAGGTTATGGGATGTAGAAACTGGTGAAGAGTTGCTTCTACAAGAAGGTCACAGTAGGAGTGTCTATGGGATAGACTTCCATCCTGATGGATCATTGGTGGCATCCTCTGGACTGGATGCACTTACTCGTGTTTGGGACCTCCGTACTGGTAGAAGTATTCTTGCCTTGGAAGGCCATGTCAAGAAAGTTCTTGCACTCAGTTTCTCAGCAAATGGCTATTATTTAGCCACTGGGGGTGAAGACAACACTTGCAGAATATGGGATTTGAGAAAAAAGAAATCCATATACACCATTCCAGCACATTCCAATCTCATATCACAGGTCAAATTTGAGCCACAAGAGGGATATTTCCTTGTAACTGCTTCATATGATTTGACAGCAAAGATATGGTCAGCCCGAGATTTTAAGCCTGTCAAGACGCTTTCTGGACATGAAGCAAAAGTTACATCTTTGGATATTTCTGGAGATGGGCAACAGATTGCTACTGTATCACATGACCGAACCATCAAACTGTGGTCCAGCCGTTCCAACAAGAAGGATCAAGCAATGGATGTGGATTACAGTACCAATGAGAAGGATCAGGCAATGGATGCGGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0001709 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007154 GO:0007267 GO:0007275 GO:0007276 GO:0007292 GO:0008150 GO:0008152 GO:0008380 GO:0009553 GO:0009560 GO:0009561 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016604 GO:0016607 GO:0017069 GO:0017070 GO:0019953 GO:0022412 GO:0022414 GO:0023052 GO:0030154 GO:0030312 GO:0030532 GO:0030621 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044703 GO:0045165 GO:0046483 GO:0046540 GO:0048229 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0070013 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

PRP4 WD40

Protein Analysis

543

Amino Acids

60.34

Weight (kDa)

5.4

Isoelectric Point (pI)

42.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRP4	PF08799	102 - 128	1e-11	pre-mRNA processing factor 4 (PRP4) like
WD40_CDC20-Fz	PF24807	228 - 515	6.7e-39	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd	PF25172	232 - 350	6.9e-12	WDR3 second beta-propeller domain
EIF3I	PF24805	234 - 310	4.2e-09	EIF3I
Beta-prop_WDR5	PF25175	236 - 396	1.4e-40	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	238 - 354	3.2e-13	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	239 - 345	7.3e-10	G protein beta WD-40 repeat protein
Beta-prop_THOC3	PF25174	240 - 354	6.1e-22	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	240 - 390	2.5e-25	WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	242 - 312	1.4e-06	CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st	PF24817	242 - 311	1.3e-08	WDHD1 first WD40 domain
WD40_Prp19	PF24814	246 - 467	2.5e-59	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	261 - 354	5e-12	WDHD1 first WD40 domain
WD40	PF00400	269 - 306	5e-09	WD domain, G-beta repeat
Beta-prop_TEP1_2nd	PF25047	269 - 391	5.3e-08	TEP-1 second beta-propeller
WDR55	PF24796	279 - 400	1e-10	WDR55
WD40_MABP1-WDR62_2nd	PF24782	280 - 390	1.3e-11	MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd	PF25047	292 - 474	8e-12	TEP-1 second beta-propeller
WD40	PF00400	309 - 347	2.9e-06	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	314 - 396	1.5e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR90_POC16_2nd	PF23393	332 - 418	1e-05	WDR90/POC16, second beta-propeller
WD40_Gbeta	PF25391	336 - 515	5.3e-29	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_1st	PF25171	342 - 473	6.6e-09	WDR36/Utp21 first beta-propeller
WDR55	PF24796	354 - 474	1.1e-12	WDR55
EIF3I	PF24805	356 - 444	1.7e-10	EIF3I
WD40	PF00400	357 - 389	2.8e-07	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	358 - 457	1.7e-21	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	358 - 516	3.3e-32	WDR3 first beta-propeller domain
Beta-prop_EML	PF23409	359 - 464	1.7e-10	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd	PF25172	363 - 517	6.9e-26	WDR3 second beta-propeller domain
Beta-prop_EML_2	PF23414	364 - 531	1.6e-21	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	367 - 527	6.5e-12	MABP1/WDR62 second WD40 domain
Beta-prop_DCAF4	PF23761	370 - 445	7.8e-06	DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_WDR5	PF25175	393 - 522	1.7e-28	WDR5 beta-propeller domain
WD40	PF00400	395 - 431	1.5e-09	WD domain, G-beta repeat
Beta-prop_TEP1_2nd	PF25047	397 - 517	9.1e-12	TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	398 - 475	5.9e-10	CAF1B/HIR1 beta-propeller domain
WD40_MABP1-WDR62_1st	PF24780	398 - 524	2.4e-11	MABP1/WDR62 first WD40 domain
Beta-prop_THOC3	PF25174	402 - 534	5.3e-27	THOC3 beta-propeller domain
Beta-prop_EIPR1	PF23609	403 - 473	8.8e-06	EIPR1 beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	418 - 502	6.3e-06	WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	420 - 519	2.6e-08	WDR36/Utp21 second beta-propeller domain
EIF3I	PF24805	443 - 527	9.8e-09	EIF3I
WD40_Prp19	PF24814	449 - 535	2.8e-11	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	456 - 528	4.4e-10	WDHD1 first WD40 domain
WD40	PF00400	479 - 516	2.3e-10	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006652)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G05720 AT2G41500 AT2G41500
fragaria_vesca	FvH4_6g24750
malus_domestica	MD12G1086300.v1.1 MD14G1080500.v1.1
prunus_persica	Prupe.7G050300_v2.0.a1 Prupe.7G050300_v2.0.a1
pyrus_communis	pycom06g09480 pycom12g07290
rosa_chinensis	RchiOBHm_Chr3g0484331 RchiOBHm_Chr3g0484401 RchiOBHm_Chr3g0484501
rosa_laevigata	RLG00000023231
rosa_multiflora	Rmu_co8232775.1_g000001 Rmu_sc0000295.1_g000017 Rmu_sc0000295.1_g000018 Rmu_sc0001947.1_g000006
rosa_roxburghii	Rroxscaffold_6G00397770
rosa_rugosa	Rorug03G0210900
rosa_samantha	Rh3AG260000 Rh3AG261700 Rh3BG296600 Rh3CG294200 Rh3DG290300
rosa_wichuraiana	Rw3G023450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	104, 786
AccBSI	CCGCTC	1 cut(s)	226
AccII	CGCG	1 cut(s)	72
AciI	CCGC	9 cut(s)	70, 89, 92, 95, 226, 322, 581, 589, 1622
AclWI	GGATC	3 cut(s)	1126, 1575, 1614
AcoI	YGGCCR	2 cut(s)	300, 777
AcsI	RAATTY	1 cut(s)	1355
AcuI	CTGAAG	2 cut(s)	540, 819
AfaI	GTAC	7 cut(s)	191, 506, 566, 998, 1006, 1174, 1594
AfiI	CCNNNNNNNGG	6 cut(s)	104, 576, 628, 719, 786, 1370
AgsI	TTSAA	2 cut(s)	433, 541
AjnI	CCWGG	1 cut(s)	113
AluBI	AGCT	5 cut(s)	167, 461, 533, 934, 1014
AluI	AGCT	5 cut(s)	167, 461, 533, 934, 1014
Alw21I	GWGCWC	1 cut(s)	125
Alw26I	GTCTC	1 cut(s)	366
AlwI	GGATC	3 cut(s)	1126, 1575, 1614
AlwNI	CAGNNNCTG	1 cut(s)	250
Ama87I	CYCGRG	1 cut(s)	1425
AoxI	GGCC	6 cut(s)	300, 582, 710, 777, 949, 1201
ApeKI	GCWGC	4 cut(s)	47, 455, 458, 470
ApoI	RAATTY	1 cut(s)	1355
ArsI	GACNNNNNNTTYG	2 cut(s)	1403, 1435
AspLEI	GCGC	2 cut(s)	74, 264
AspS9I	GGNCC	5 cut(s)	525, 949, 958, 1165, 1545
AsuHPI	GGTGA	4 cut(s)	229, 497, 1064, 1277
AvaI	CYCGRG	1 cut(s)	1425
AvaII	GGWCC	4 cut(s)	525, 958, 1165, 1545
BalI	TGGCCA	1 cut(s)	779
BanII	GRGCYC	2 cut(s)	313, 604
BauI	CACGAG	2 cut(s)	162, 1155
BbsI	GAAGAC	2 cut(s)	308, 1275
Bbv12I	GWGCWC	1 cut(s)	125
BbvI	GCAGC	4 cut(s)	59, 442, 457, 470
BceAI	ACGGC	3 cut(s)	249, 287, 1536
BciT130I	CCWGG	1 cut(s)	115
BclI	TGATCA	2 cut(s)	387, 490
BcoDI	GTCTC	1 cut(s)	366
BfaI	CTAG	2 cut(s)	546, 1011
BfmI	CTRYAG	2 cut(s)	456, 471
BfoI	RGCGCY	1 cut(s)	265
BglI	GCCNNNNNGGC	1 cut(s)	308
BmcAI	AGTACT	2 cut(s)	566, 998
Bme1390I	CCNGG	1 cut(s)	115
Bme18I	GGWCC	4 cut(s)	525, 958, 1165, 1545
BmeT110I	CYCGRG	1 cut(s)	1425
BmgT120I	GGNCC	5 cut(s)	525, 949, 958, 1165, 1545
BmiI	GGNNCC	4 cut(s)	36, 156, 526, 1166
BmrFI	CCNGG	1 cut(s)	115
BmrI	ACTGGG	1 cut(s)	1269
BmsI	GCATC	4 cut(s)	628, 1135, 1139, 1609
BmuI	ACTGGG	1 cut(s)	1269
BpiI	GAAGAC	2 cut(s)	308, 1275
BpmI	CTGGAG	2 cut(s)	48, 1512
BsaJI	CCNNGG	1 cut(s)	1194
BsaWI	WCCGGW	2 cut(s)	31, 897
BsaXI	ACNNNNNCTCC	2 cut(s)	413, 443
Bsc4I	CCNNNNNNNGG	6 cut(s)	104, 576, 628, 719, 786, 1370
Bse118I	RCCGGY	1 cut(s)	170
Bse1I	ACTGG	6 cut(s)	65, 98, 1054, 1146, 1180, 1264
Bse3DI	GCAATG	4 cut(s)	246, 555, 1581, 1620
BseBI	CCWGG	1 cut(s)	115
BseDI	CCNNGG	1 cut(s)	1194
BseLI	CCNNNNNNNGG	6 cut(s)	104, 576, 628, 719, 786, 1370
BseMI	GCAATG	4 cut(s)	246, 555, 1581, 1620
BseMII	CTCAG	4 cut(s)	97, 944, 1240, 1248
BseNI	ACTGG	6 cut(s)	65, 98, 1054, 1146, 1180, 1264
BseRI	GAGGAG	7 cut(s)	20, 23, 66, 167, 458, 461, 464
BseXI	GCAGC	4 cut(s)	59, 442, 457, 470
Bsh1236I	CGCG	1 cut(s)	72
BshFI	GGCC	6 cut(s)	302, 584, 712, 779, 951, 1203
BsiHKAI	GWGCWC	1 cut(s)	125
BsiHKCI	CYCGRG	1 cut(s)	1425
BsiSI	CCGG	3 cut(s)	32, 171, 898
BslFI	GGGAC	3 cut(s)	38, 840, 1178
BslI	CCNNNNNNNGG	6 cut(s)	104, 576, 628, 719, 786, 1370
BsmAI	GTCTC	1 cut(s)	366
BsmFI	GGGAC	3 cut(s)	38, 840, 1178
BsmI	GAATGC	1 cut(s)	547
BsnI	GGCC	6 cut(s)	302, 584, 712, 779, 951, 1203
BsoBI	CYCGRG	1 cut(s)	1425
Bsp1286I	GDGCHC	3 cut(s)	125, 313, 604
Bsp143I	GATC	5 cut(s)	387, 490, 1118, 1567, 1606
BspACI	CCGC	9 cut(s)	70, 89, 92, 95, 226, 322, 581, 589, 1622
BspANI	GGCC	6 cut(s)	302, 584, 712, 779, 951, 1203
BspCNI	CTCAG	4 cut(s)	96, 943, 1239, 1247
BspFNI	CGCG	1 cut(s)	72
BspHI	TCATGA	1 cut(s)	439
BspLI	GGNNCC	4 cut(s)	36, 156, 526, 1166
BspMAI	CTGCAG	2 cut(s)	460, 475
BspPI	GGATC	3 cut(s)	1126, 1575, 1614
BsrBI	CCGCTC	1 cut(s)	226
BsrDI	GCAATG	4 cut(s)	246, 555, 1581, 1620
BsrFI	RCCGGY	1 cut(s)	170
BsrI	ACTGG	6 cut(s)	65, 98, 1054, 1146, 1180, 1264
BssAI	RCCGGY	1 cut(s)	170
BssECI	CCNNGG	1 cut(s)	1194
BssMI	GATC	5 cut(s)	387, 490, 1118, 1567, 1606
BssSI	CACGAG	2 cut(s)	162, 1155
BssT1I	CCWWGG	1 cut(s)	1194
Bst2BI	CACGAG	2 cut(s)	162, 1155
Bst2UI	CCWGG	1 cut(s)	115
Bst4CI	ACNGT	5 cut(s)	962, 1082, 1516, 1542, 1592
Bst6I	CTCTTC	3 cut(s)	198, 350, 1050
BstAPI	GCANNNNNTGC	3 cut(s)	250, 974, 1619
BstC8I	GCNNGC	3 cut(s)	666, 753, 794
BstDEI	CTNAG	5 cut(s)	83, 877, 930, 1226, 1234
BstEII	GGTNACC	1 cut(s)	811
BstFNI	CGCG	1 cut(s)	72
BstH2I	RGCGCY	1 cut(s)	265
BstHHI	GCGC	2 cut(s)	74, 264
BstKTI	GATC	5 cut(s)	390, 493, 1121, 1570, 1609
BstMAI	GTCTC	1 cut(s)	366
BstMBI	GATC	5 cut(s)	387, 490, 1118, 1567, 1606
BstMWI	GCNNNNNNNGC	8 cut(s)	250, 259, 308, 406, 467, 761, 974, 1619
BstNI	CCWGG	1 cut(s)	115
BstNSI	RCATGY	1 cut(s)	796
BstPI	GGTNACC	1 cut(s)	811
BstSCI	CCNGG	1 cut(s)	113
BstSFI	CTRYAG	2 cut(s)	456, 471
BstUI	CGCG	1 cut(s)	72
BstV1I	GCAGC	4 cut(s)	59, 442, 457, 470
BstV2I	GAAGAC	2 cut(s)	308, 1275
BstXI	CCANNNNNNTGG	2 cut(s)	298, 1115
BsuRI	GGCC	6 cut(s)	302, 584, 712, 779, 951, 1203
BtsI	GCAGTG	2 cut(s)	480, 697
BtsIMutI	CAGTG	4 cut(s)	480, 516, 697, 1257
Cac8I	GCNNGC	3 cut(s)	666, 753, 794
CaiI	CAGNNNCTG	1 cut(s)	250
CciI	TCATGA	1 cut(s)	439
CfoI	GCGC	2 cut(s)	74, 264
Cfr10I	RCCGGY	1 cut(s)	170
Cfr13I	GGNCC	5 cut(s)	525, 949, 958, 1165, 1545
CseI	GACGC	1 cut(s)	1456
Csp6I	GTAC	7 cut(s)	190, 505, 565, 997, 1005, 1173, 1593
CspCI	CAANNNNNGTGG	2 cut(s)	1563, 1598
CviAII	CATG	8 cut(s)	298, 391, 440, 737, 793, 1205, 1460, 1523
CviQI	GTAC	7 cut(s)	190, 505, 565, 997, 1005, 1173, 1593
DdeI	CTNAG	5 cut(s)	83, 877, 930, 1226, 1234
DpnI	GATC	5 cut(s)	389, 492, 1120, 1569, 1608
DpnII	GATC	5 cut(s)	387, 490, 1118, 1567, 1606
DraI	TTTAAA	1 cut(s)	822
EaeI	YGGCCR	2 cut(s)	300, 777
Eam1104I	CTCTTC	3 cut(s)	198, 350, 1050
EarI	CTCTTC	3 cut(s)	198, 350, 1050
Eco130I	CCWWGG	1 cut(s)	1194
Eco24I	GRGCYC	2 cut(s)	313, 604
Eco32I	GATATC	1 cut(s)	198
Eco47I	GGWCC	4 cut(s)	525, 958, 1165, 1545
Eco57I	CTGAAG	2 cut(s)	540, 819
Eco88I	CYCGRG	1 cut(s)	1425
Eco91I	GGTNACC	1 cut(s)	811
EcoO109I	RGGNCCY	1 cut(s)	1165
EcoO65I	GGTNACC	1 cut(s)	811
EcoRII	CCWGG	1 cut(s)	113
EcoRV	GATATC	1 cut(s)	198
EcoT14I	CCWWGG	1 cut(s)	1194
EcoT38I	GRGCYC	2 cut(s)	313, 604
ErhI	CCWWGG	1 cut(s)	1194
FaeI	CATG	8 cut(s)	301, 394, 443, 740, 796, 1208, 1463, 1526
FalI	AAGNNNNNCTT	2 cut(s)	1047, 1079
FaqI	GGGAC	3 cut(s)	38, 840, 1178
FatI	CATG	8 cut(s)	297, 390, 439, 736, 792, 1204, 1459, 1522
FauNDI	CATATG	1 cut(s)	1396
FbaI	TGATCA	2 cut(s)	387, 490
FriOI	GRGCYC	2 cut(s)	313, 604
FspBI	CTAG	2 cut(s)	546, 1011
GlaI	GCGC	2 cut(s)	73, 263
GsuI	CTGGAG	2 cut(s)	48, 1512
HaeII	RGCGCY	1 cut(s)	265
HaeIII	GGCC	6 cut(s)	302, 584, 712, 779, 951, 1203
HapII	CCGG	3 cut(s)	32, 171, 898
HgaI	GACGC	1 cut(s)	1456
HhaI	GCGC	2 cut(s)	74, 264
Hin1II	CATG	8 cut(s)	301, 394, 443, 740, 796, 1208, 1463, 1526
Hin6I	GCGC	2 cut(s)	72, 262
HinP1I	GCGC	2 cut(s)	72, 262
HincII	GTYRAC	1 cut(s)	939
HindII	GTYRAC	1 cut(s)	939
HindIII	AAGCTT	1 cut(s)	531
HinfI	GANTC	4 cut(s)	131, 140, 146, 404
HpaII	CCGG	3 cut(s)	32, 171, 898
HphI	GGTGA	4 cut(s)	229, 497, 1064, 1277
Hpy166II	GTNNAC	1 cut(s)	939
Hpy188I	TCNGA	5 cut(s)	86, 136, 145, 151, 202
Hpy188III	TCNNGA	7 cut(s)	440, 1112, 1137, 1210, 1444, 1455, 1491
Hpy8I	GTNNAC	1 cut(s)	939
HpyAV	CCTTC	9 cut(s)	515, 607, 802, 917, 988, 1067, 1193, 1558, 1597
HpyCH4III	ACNGT	5 cut(s)	962, 1082, 1516, 1542, 1592
HpyF10VI	GCNNNNNNNGC	8 cut(s)	250, 259, 308, 406, 467, 761, 974, 1619
HpyF3I	CTNAG	5 cut(s)	83, 877, 930, 1226, 1234
Hsp92II	CATG	8 cut(s)	301, 394, 443, 740, 796, 1208, 1463, 1526
HspAI	GCGC	2 cut(s)	72, 262
Ksp22I	TGATCA	2 cut(s)	387, 490
Kzo9I	GATC	5 cut(s)	387, 490, 1118, 1567, 1606
LmnI	GCTCC	6 cut(s)	34, 67, 79, 154, 661, 789
Lsp1109I	GCAGC	4 cut(s)	59, 442, 457, 470
LweI	GCATC	4 cut(s)	628, 1135, 1139, 1609
MaeI	CTAG	2 cut(s)	546, 1011
MaeIII	GTNAC	5 cut(s)	128, 811, 1076, 1384, 1471
MalI	GATC	5 cut(s)	389, 492, 1120, 1569, 1608
MbiI	CCGCTC	1 cut(s)	226
MboI	GATC	5 cut(s)	387, 490, 1118, 1567, 1606
MboII	GAAGA	6 cut(s)	215, 308, 337, 618, 1067, 1280
MfeI	CAATTG	1 cut(s)	57
MhlI	GDGCHC	3 cut(s)	125, 313, 604
MlsI	TGGCCA	1 cut(s)	779
MluCI	AATT	4 cut(s)	57, 495, 972, 1355
MluNI	TGGCCA	1 cut(s)	779
MlyI	GAGTC	2 cut(s)	125, 140
MmeI	TCCRAC	4 cut(s)	168, 399, 627, 1581
Mox20I	TGGCCA	1 cut(s)	779
MscI	TGGCCA	1 cut(s)	779
MseI	TTAA	3 cut(s)	821, 945, 1434
MslI	CAYNNNNRTG	2 cut(s)	302, 1113
Msp20I	TGGCCA	1 cut(s)	779
MspA1I	CMGCKG	1 cut(s)	934
MspI	CCGG	3 cut(s)	32, 171, 898
MspR9I	CCNGG	1 cut(s)	115
MunI	CAATTG	1 cut(s)	57
Mva1269I	GAATGC	1 cut(s)	547
MvaI	CCWGG	1 cut(s)	115
MvnI	CGCG	1 cut(s)	72
MwoI	GCNNNNNNNGC	8 cut(s)	250, 259, 308, 406, 467, 761, 974, 1619
NdeI	CATATG	1 cut(s)	1396
NdeII	GATC	5 cut(s)	387, 490, 1118, 1567, 1606
NlaIII	CATG	8 cut(s)	301, 394, 443, 740, 796, 1208, 1463, 1526
NlaIV	GGNNCC	4 cut(s)	36, 156, 526, 1166
NmeAIII	GCCGAG	1 cut(s)	306
NmuCI	GTSAC	2 cut(s)	128, 1076
NspI	RCATGY	1 cut(s)	796
PaeI	GCATGC	1 cut(s)	796
PagI	TCATGA	1 cut(s)	439
PcsI	WCGNNNNNNNCGW	1 cut(s)	300
PctI	GAATGC	1 cut(s)	547
PfeI	GAWTC	2 cut(s)	140, 404
PflMI	CCANNNNNTGG	2 cut(s)	104, 786
PleI	GAGTC	2 cut(s)	125, 140
PpsI	GAGTC	2 cut(s)	125, 140
PpuMI	RGGWCCY	1 cut(s)	1165
Psp5II	RGGWCCY	1 cut(s)	1165
Psp6I	CCWGG	1 cut(s)	113
PspEI	GGTNACC	1 cut(s)	811
PspGI	CCWGG	1 cut(s)	113
PspN4I	GGNNCC	4 cut(s)	36, 156, 526, 1166
PspPI	GGNCC	5 cut(s)	525, 949, 958, 1165, 1545
PspPPI	RGGWCCY	1 cut(s)	1165
PstI	CTGCAG	2 cut(s)	460, 475
PstNI	CAGNNNCTG	1 cut(s)	250
PvuII	CAGCTG	1 cut(s)	934
RsaI	GTAC	7 cut(s)	191, 506, 566, 998, 1006, 1174, 1594
RsaNI	GTAC	7 cut(s)	190, 505, 565, 997, 1005, 1173, 1593
RseI	CAYNNNNRTG	2 cut(s)	302, 1113
SaqAI	TTAA	3 cut(s)	821, 945, 1434
Sau3AI	GATC	5 cut(s)	387, 490, 1118, 1567, 1606
Sau96I	GGNCC	5 cut(s)	525, 949, 958, 1165, 1545
ScaI	AGTACT	2 cut(s)	566, 998
SchI	GAGTC	2 cut(s)	125, 140
ScrFI	CCNGG	1 cut(s)	115
SduI	GDGCHC	3 cut(s)	125, 313, 604
SfaNI	GCATC	4 cut(s)	628, 1135, 1139, 1609
SfcI	CTRYAG	2 cut(s)	456, 471
SinI	GGWCC	4 cut(s)	525, 958, 1165, 1545
SmiMI	CAYNNNNRTG	2 cut(s)	302, 1113
SphI	GCATGC	1 cut(s)	796
Sse9I	AATT	4 cut(s)	57, 495, 972, 1355
SsiI	CCGC	9 cut(s)	70, 89, 92, 95, 226, 322, 581, 589, 1622
SspMI	CTAG	2 cut(s)	546, 1011
StyD4I	CCNGG	1 cut(s)	113
StyI	CCWWGG	1 cut(s)	1194
TaaI	ACNGT	5 cut(s)	962, 1082, 1516, 1542, 1592
TaqI	TCGA	2 cut(s)	594, 604
TaqII	GACCGA	1 cut(s)	1542
TasI	AATT	4 cut(s)	57, 495, 972, 1355
TatI	WGTACW	2 cut(s)	564, 996
TauI	GCSGC	6 cut(s)	94, 97, 229, 325, 584, 592
TfiI	GAWTC	2 cut(s)	140, 404
Tru1I	TTAA	3 cut(s)	821, 945, 1434
Tru9I	TTAA	3 cut(s)	821, 945, 1434
TscAI	CASTG	4 cut(s)	480, 523, 697, 1264
TseFI	GTSAC	2 cut(s)	128, 1076
TseI	GCWGC	4 cut(s)	47, 455, 458, 470
Tsp45I	GTSAC	2 cut(s)	128, 1076
TspDTI	ATGAA	4 cut(s)	272, 428, 1383, 1476
TspGWI	ACGGA	1 cut(s)	1160
TspRI	CASTG	4 cut(s)	480, 523, 697, 1264
Van91I	CCANNNNNTGG	2 cut(s)	104, 786
VpaK11BI	GGWCC	4 cut(s)	525, 958, 1165, 1545
XapI	RAATTY	1 cut(s)	1355
XceI	RCATGY	1 cut(s)	796
XcmI	CCANNNNNNNNNTGG	1 cut(s)	295
XspI	CTAG	2 cut(s)	546, 1011
ZrmI	AGTACT	2 cut(s)	566, 998

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.