Rh3AG261700

U4 U6 small nuclear ribonucleoprotein PRP4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0006652
Physical Location & Seq
Forward (+)
29104825 .. 29114640
9816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG261700.1

Sequence Viewer

Length: 921 bp
ATGGATGCCGAAATCAACTGGGCTTTAGAGCAGGCCTCCAATTTGAAGCTTGATTGCAGTGAGATTGGGGATGATAGGCCACTTTCTGGTTGCTCATTCTCACAAGATGGCAAATTGCTTGCCGCATGCGCTCTAAGTCCAATTGCCAAGCTGTGGAGCATGCCTCAAGTAACAAAGGTTGCCACCTTGAAGGGACATACAGAGCGCTTAACCGATGTTGCATTTTCTCCTGTGCAAAATCATCTCTTGGCAACTGCGTCTGCGGATAGGACTGCGAGGTTGTGGAACAGCGAAGGAGCACACCTAATGACATTCGGGGGACATTTGGACCGTCTTGCCCGCATTGCCTTCCATCCCTCAGGAAAGTACTTGGGTACAACTAGCTTTGACAAGACTTGGAGGCTGTGGGATGTAGAGACTGGTGAAGAGTTGCTTCTTCAAGAAGGTCACAGTAGGAGTGTCTATGCTAAGACTTCCACCCAGATGGCTCATTGGTTGCAACTGCTGGATTGGATGCGCTTGCTCGTGTTTGGGACCTCCGTAGTGGTTCTTGCACTCAGTTTTTCACCAAATGGTTATTATCTAGCCCCTGGGGGTGAAGACAACACTTGTAGGATATGGGATTTAAGGAAAAAGAAATCCATATACATTATTCCAGCACACTCCAATCTCATATCACAGGTCAAATTTGAACCTCAAGAGGGATATTTCTTGGTAACTGGCTCCTATGATATGACAGCAAAGATATGGTCAGCCCGAGATTTTAAGCCTGTCAAGACGCTCTCTGGACACGGATCAAAAGTTACTTCCTTGGATGTTTCTGGTGATGGACATCACATTGCTACTGTATCACATGACCGAACTATCAAACTATGGTCGAGCAGTACCAATGAGAAGGACCAGGAAATGGATGTTGATTAG

Protein Analysis

306

Amino Acids

34.03

Weight (kDa)

6.3

Isoelectric Point (pI)

32.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 16 - 140 1e-11 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 23 - 161 4.8e-12 G protein beta WD-40 repeat protein
EIF3I PF24805 23 - 98 3.9e-07 EIF3I
Beta-prop_WDR3_1st PF25173 23 - 159 1.5e-17 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 25 - 156 2.8e-26 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 26 - 165 2.5e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 26 - 102 2.9e-08 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 31 - 98 1.2e-07 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 31 - 141 1.6e-19 THOC3 beta-propeller domain
WD40_Prp19 PF24814 38 - 151 1.4e-20 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 56 - 159 4e-06 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 58 - 142 3.4e-09 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 59 - 96 3.5e-08 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 81 - 159 7.2e-08 WDHD1 first WD40 domain
WD40 PF00400 101 - 137 4.3e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 102 - 153 7.5e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 174 - 296 4e-20 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 178 - 292 9.3e-16 G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd PF25047 179 - 298 2e-11 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 181 - 293 2.3e-21 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 182 - 298 2.6e-11 MABP1/WDR62 first WD40 domain
WD40_Prp19 PF24814 182 - 241 5.7e-14 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 183 - 298 5.7e-26 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 183 - 236 5.9e-06 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 183 - 259 1.2e-10 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 183 - 303 1e-23 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 184 - 301 1.2e-14 Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 184 - 299 6.2e-08 MABP1/WDR62 second WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 195 - 279 7.9e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 199 - 294 9.3e-07 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 220 - 300 1e-08 EIF3I
WD40_Prp19 PF24814 225 - 293 8.4e-12 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 233 - 302 1.4e-10 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 247 - 292 1e-06 CDC20/Fizzy WD40 domain
WD40 PF00400 256 - 293 2e-10 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 86, 153, 907
AciI CCGC 3 cut(s) 123, 263, 340
AclWI GGATC 1 cut(s) 802
AcsI RAATTY 1 cut(s) 686
AfaI GTAC 3 cut(s) 368, 376, 886
AfeI AGCGCT 1 cut(s) 206
AfiI CCNNNNNNNGG 4 cut(s) 86, 153, 701, 907
AgsI TTSAA 4 cut(s) 46, 190, 440, 692
AjnI CCWGG 2 cut(s) 589, 900
AluBI AGCT 3 cut(s) 49, 151, 384
AluI AGCT 3 cut(s) 49, 151, 384
Alw21I GWGCWC 1 cut(s) 301
Alw26I GTCTC 1 cut(s) 410
AlwI GGATC 1 cut(s) 802
Ama87I CYCGRG 1 cut(s) 756
Aor51HI AGCGCT 1 cut(s) 206
AoxI GGCC 2 cut(s) 33, 77
ApoI RAATTY 1 cut(s) 686
ArsI GACNNNNNNTTYG 2 cut(s) 734, 766
AspLEI GCGC 3 cut(s) 131, 207, 519
AspS9I GGNCC 3 cut(s) 328, 534, 898
AsuHPI GGTGA 4 cut(s) 434, 558, 608, 836
AvaI CYCGRG 1 cut(s) 756
AvaII GGWCC 3 cut(s) 328, 534, 898
AxyI CCTNAGG 1 cut(s) 358
BauI CACGAG 1 cut(s) 524
BbsI GAAGAC 1 cut(s) 606
Bbv12I GWGCWC 1 cut(s) 301
BccI CCATC 4 cut(s) 101, 360, 478, 821
BciT130I CCWGG 2 cut(s) 591, 902
BcoDI GTCTC 1 cut(s) 410
BfaI CTAG 2 cut(s) 381, 584
BfoI RGCGCY 1 cut(s) 208
BisI GCNGC 1 cut(s) 123
BlsI GCNGC 1 cut(s) 124
BmcAI AGTACT 1 cut(s) 368
Bme1390I CCNGG 2 cut(s) 591, 902
Bme18I GGWCC 3 cut(s) 328, 534, 898
BmeT110I CYCGRG 1 cut(s) 756
BmgT120I GGNCC 3 cut(s) 328, 534, 898
BmiI GGNNCC 2 cut(s) 535, 724
BmrFI CCNGG 2 cut(s) 591, 902
BmrI ACTGGG 1 cut(s) 28
BmsI GCATC 1 cut(s) 504
BmuI ACTGGG 1 cut(s) 28
BpiI GAAGAC 1 cut(s) 606
BplI GAGNNNNNCTC 4 cut(s) 20, 52, 148, 180
BpuEI CTTGAG 2 cut(s) 150, 681
BsaBI GATNNNNATC 1 cut(s) 831
BsaJI CCNNGG 3 cut(s) 589, 590, 810
Bsc4I CCNNNNNNNGG 4 cut(s) 86, 153, 701, 907
Bse1I ACTGG 3 cut(s) 23, 424, 724
Bse21I CCTNAGG 1 cut(s) 358
Bse3DI GCAATG 2 cut(s) 342, 837
Bse8I GATNNNNATC 1 cut(s) 831
BseBI CCWGG 2 cut(s) 591, 902
BseDI CCNNGG 3 cut(s) 589, 590, 810
BseGI GGATG 7 cut(s) 10, 76, 352, 415, 519, 820, 916
BseJI GATNNNNATC 1 cut(s) 831
BseLI CCNNNNNNNGG 4 cut(s) 86, 153, 701, 907
BseMI GCAATG 2 cut(s) 342, 837
BseMII CTCAG 2 cut(s) 372, 571
BseNI ACTGG 3 cut(s) 23, 424, 724
BshFI GGCC 2 cut(s) 35, 79
BsiHKAI GWGCWC 1 cut(s) 301
BsiHKCI CYCGRG 1 cut(s) 756
BslFI GGGAC 3 cut(s) 207, 333, 547
BslI CCNNNNNNNGG 4 cut(s) 86, 153, 701, 907
BsmAI GTCTC 1 cut(s) 410
BsmFI GGGAC 3 cut(s) 207, 333, 547
BsnI GGCC 2 cut(s) 35, 79
BsoBI CYCGRG 1 cut(s) 756
Bsp1286I GDGCHC 1 cut(s) 301
Bsp143I GATC 1 cut(s) 794
BspACI CCGC 3 cut(s) 123, 263, 340
BspANI GGCC 2 cut(s) 35, 79
BspCNI CTCAG 2 cut(s) 371, 570
BspLI GGNNCC 2 cut(s) 535, 724
BspPI GGATC 1 cut(s) 802
BsrDI GCAATG 2 cut(s) 342, 837
BsrI ACTGG 3 cut(s) 23, 424, 724
BssECI CCNNGG 3 cut(s) 589, 590, 810
BssMI GATC 1 cut(s) 794
BssSI CACGAG 1 cut(s) 524
BssT1I CCWWGG 1 cut(s) 810
Bst2BI CACGAG 1 cut(s) 524
Bst2UI CCWGG 2 cut(s) 591, 902
Bst4CI ACNGT 3 cut(s) 332, 452, 847
Bst6I CTCTTC 1 cut(s) 420
BstC8I GCNNGC 6 cut(s) 33, 120, 127, 161, 340, 521
BstDEI CTNAG 4 cut(s) 134, 358, 468, 557
BstF5I GGATG 7 cut(s) 10, 76, 352, 415, 519, 820, 916
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 3 cut(s) 131, 207, 519
BstKTI GATC 1 cut(s) 797
BstMAI GTCTC 1 cut(s) 410
BstMBI GATC 1 cut(s) 794
BstMWI GCNNNNNNNGC 2 cut(s) 128, 344
BstNI CCWGG 2 cut(s) 591, 902
BstNSI RCATGY 2 cut(s) 129, 163
BstSCI CCNGG 2 cut(s) 589, 900
BstV2I GAAGAC 1 cut(s) 606
BstXI CCANNNNNNTGG 1 cut(s) 484
Bsu36I CCTNAGG 1 cut(s) 358
BsuRI GGCC 2 cut(s) 35, 79
BtsCI GGATG 7 cut(s) 10, 76, 352, 415, 519, 820, 916
BtsI GCAGTG 1 cut(s) 64
BtsIMutI CAGTG 1 cut(s) 64
Cac8I GCNNGC 6 cut(s) 33, 120, 127, 161, 340, 521
CfoI GCGC 3 cut(s) 131, 207, 519
Cfr13I GGNCC 3 cut(s) 328, 534, 898
CseI GACGC 2 cut(s) 246, 787
Csp6I GTAC 3 cut(s) 367, 375, 885
CviAII CATG 3 cut(s) 126, 160, 854
CviQI GTAC 3 cut(s) 367, 375, 885
DdeI CTNAG 4 cut(s) 134, 358, 468, 557
DpnI GATC 1 cut(s) 796
DpnII GATC 1 cut(s) 794
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
Eco130I CCWWGG 1 cut(s) 810
Eco147I AGGCCT 1 cut(s) 35
Eco47I GGWCC 3 cut(s) 328, 534, 898
Eco47III AGCGCT 1 cut(s) 206
Eco81I CCTNAGG 1 cut(s) 358
Eco88I CYCGRG 1 cut(s) 756
EcoO109I RGGNCCY 1 cut(s) 534
EcoRII CCWGG 2 cut(s) 589, 900
EcoT14I CCWWGG 1 cut(s) 810
ErhI CCWWGG 1 cut(s) 810
FaeI CATG 3 cut(s) 129, 163, 857
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 3 cut(s) 207, 333, 547
FatI CATG 3 cut(s) 125, 159, 853
FauI CCCGC 1 cut(s) 347
Fnu4HI GCNGC 1 cut(s) 123
FokI GGATG 6 cut(s) 17, 83, 339, 422, 526, 827
Fsp4HI GCNGC 1 cut(s) 123
FspBI CTAG 2 cut(s) 381, 584
GlaI GCGC 3 cut(s) 130, 206, 518
GluI GCNGC 1 cut(s) 123
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 2 cut(s) 35, 79
HgaI GACGC 2 cut(s) 246, 787
HhaI GCGC 3 cut(s) 131, 207, 519
Hin1II CATG 3 cut(s) 129, 163, 857
Hin6I GCGC 3 cut(s) 129, 205, 517
HinP1I GCGC 3 cut(s) 129, 205, 517
HindIII AAGCTT 1 cut(s) 47
HphI GGTGA 4 cut(s) 434, 558, 608, 836
Hpy188III TCNNGA 5 cut(s) 360, 440, 698, 775, 786
HpyAV CCTTC 5 cut(s) 184, 287, 358, 437, 889
HpyCH4III ACNGT 3 cut(s) 332, 452, 847
HpyCH4V TGCA 5 cut(s) 57, 221, 235, 499, 554
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 344
HpyF3I CTNAG 4 cut(s) 134, 358, 468, 557
Hsp92II CATG 3 cut(s) 129, 163, 857
HspAI GCGC 3 cut(s) 129, 205, 517
Kzo9I GATC 1 cut(s) 794
LmnI GCTCC 3 cut(s) 156, 296, 728
LweI GCATC 1 cut(s) 504
MaeI CTAG 2 cut(s) 381, 584
MaeIII GTNAC 4 cut(s) 169, 446, 715, 802
MalI GATC 1 cut(s) 796
MboI GATC 1 cut(s) 794
MboII GAAGA 3 cut(s) 428, 437, 611
MfeI CAATTG 1 cut(s) 141
MhlI GDGCHC 1 cut(s) 301
MluCI AATT 4 cut(s) 40, 113, 141, 686
MnlI CCTC 8 cut(s) 46, 174, 270, 367, 393, 547, 694, 705
MseI TTAA 3 cut(s) 209, 626, 765
MslI CAYNNNNRTG 1 cut(s) 482
MspR9I CCNGG 2 cut(s) 591, 902
MunI CAATTG 1 cut(s) 141
MvaI CCWGG 2 cut(s) 591, 902
MwoI GCNNNNNNNGC 2 cut(s) 128, 344
NdeII GATC 1 cut(s) 794
NlaIII CATG 3 cut(s) 129, 163, 857
NlaIV GGNNCC 2 cut(s) 535, 724
NmuCI GTSAC 1 cut(s) 446
NspI RCATGY 2 cut(s) 129, 163
PaeI GCATGC 2 cut(s) 129, 163
PasI CCCWGGG 1 cut(s) 590
PceI AGGCCT 1 cut(s) 35
PflMI CCANNNNNTGG 3 cut(s) 86, 153, 907
PkrI GCNGC 1 cut(s) 124
PpuMI RGGWCCY 1 cut(s) 534
Psp5II RGGWCCY 1 cut(s) 534
Psp6I CCWGG 2 cut(s) 589, 900
PspGI CCWGG 2 cut(s) 589, 900
PspN4I GGNNCC 2 cut(s) 535, 724
PspPI GGNCC 3 cut(s) 328, 534, 898
PspPPI RGGWCCY 1 cut(s) 534
RsaI GTAC 3 cut(s) 368, 376, 886
RsaNI GTAC 3 cut(s) 367, 375, 885
RseI CAYNNNNRTG 1 cut(s) 482
SaqAI TTAA 3 cut(s) 209, 626, 765
SatI GCNGC 1 cut(s) 123
Sau3AI GATC 1 cut(s) 794
Sau96I GGNCC 3 cut(s) 328, 534, 898
ScaI AGTACT 1 cut(s) 368
ScrFI CCNGG 2 cut(s) 591, 902
SduI GDGCHC 1 cut(s) 301
SfaNI GCATC 1 cut(s) 504
SinI GGWCC 3 cut(s) 328, 534, 898
SmiMI CAYNNNNRTG 1 cut(s) 482
SmlI CTYRAG 2 cut(s) 165, 696
SmoI CTYRAG 2 cut(s) 165, 696
SphI GCATGC 2 cut(s) 129, 163
Sse9I AATT 4 cut(s) 40, 113, 141, 686
SseBI AGGCCT 1 cut(s) 35
SsiI CCGC 3 cut(s) 123, 263, 340
SspMI CTAG 2 cut(s) 381, 584
StuI AGGCCT 1 cut(s) 35
StyD4I CCNGG 2 cut(s) 589, 900
StyI CCWWGG 1 cut(s) 810
TaaI ACNGT 3 cut(s) 332, 452, 847
TaqI TCGA 1 cut(s) 878
TaqII GACCGA 1 cut(s) 873
TasI AATT 4 cut(s) 40, 113, 141, 686
TatI WGTACW 1 cut(s) 366
TauI GCSGC 1 cut(s) 125
Tru1I TTAA 3 cut(s) 209, 626, 765
Tru9I TTAA 3 cut(s) 209, 626, 765
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 1 cut(s) 446
Tsp45I GTSAC 1 cut(s) 446
TspGWI ACGGA 2 cut(s) 529, 807
TspRI CASTG 1 cut(s) 64
Van91I CCANNNNNTGG 3 cut(s) 86, 153, 907
VpaK11BI GGWCC 3 cut(s) 328, 534, 898
XapI RAATTY 1 cut(s) 686
XceI RCATGY 2 cut(s) 129, 163
XspI CTAG 2 cut(s) 381, 584
ZrmI AGTACT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.