Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3C

Orthogroup ID

OG0006652

Physical Location & Seq

Genomic Coordinates Forward (+)

30059138 .. 30063280

Gene Structure 4143 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3CG294200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1575 bp

ATGACAATGGAGGAGGAGGAGGAGAAGCTTCTCTCCTCTATAGCAGCTTCCGAGCCCCCCCAAAACGGCGAAGCAGCAGCCAGTACGACCGACTCCGACTCCGGTTCCGGTGACGATGCCGCCGCCGCCGCCGCTCAAGGAGCCTCCTCCGCCGGAGTCGAATACCATATATCCGAAGAGCAAAAAGCGGCGCGAGAGCGACAGGAGAGAGCAATGCAGCAGCTCTTGATGAAGCGCCGCGCTTCCGCCCTCGCCGTCCCCACCAACGACAACGCCGTCCGGACTCGTCTCCGGCGCCTCGGCGAGCCGATGACTCTGTTCGGAGAGCGAGAGATGGAAAGGCGGGACCGGCTCAGGTCGATTCTGGCCAAGCTCGATTCGGCGCAGCTGGAGACGCTGATGAAGGCTCACGACGAGGACGAGGCTTCTGCTGCTGGTGCTCTAGGCCCCGACGATTCTGACTTCGATCAAGAAATTCAGTACCCCTTCTTCACTGAAGCGACCAGGCCCAGAGCTTTGCGTGACGCTAGGGTTCATATTGCGCAGTACTCGCTTCCCAGGGCGGCGTTGCGGCTTCAACGTGCGAGGAGGAAGGAGGAGGATCCGGATGAGGATATGGATGCCGAGATCAACTGGGCTTTAGAGCAGGCCTCCAATTTGAAGCTTGATTGCAGTGAGATTGGGGATGATAGGCCACTTTCTGGTTGCTCATTCTCACAAGATGGCAAATTGCTTGCCGCCTGCGCTTTAAGTCCAGTGGCCAAGCTGTGGAGCATGCCTCAAGTAACAAAGGTTGCCACCTTGAAGGGACATACAGAGCGCTTAACCGATGTTGCATTTTCTCCTGTGCAAAATCATCTCTTGGCAACTGCGTCTGCGGATAGGACTGCGAGGTTGTGGAACAGCGAAGGAGCACACCTGATGACATTCGGGGGACATTTGGACCGTCTTGCCCGCATTGCCTTCCATCCCTCAGGAAAGTACTTGGGTACAACTAGCTTTGACAAGACTTGGAGGCTGTGGGATGTAGAGACTGGTGAAGAGTTGCTTCTTCAAGAAGGTCACAGTAGGAGTGTCTATGCTATAGACTTCCACCCAGATGGCTCATTGGTTGCAACTGCTGGATTGGATGCGCTTGCTCGTGTTTGGGACCTCCGTAGTGGTAGAAGTATTCTTACCTTGGAAGGCCATGTCAAAAAAGTTCTTGCACTCAGTTTTTCACCAAATGGTTATTATCTAGCCACTGGGGGTGAAGACAACACTTGTAGGATATGGGATTTAAGGAAAAAGAAATCCATATACATTATTCCAGCACACTCCAATCTCATATCACAGGTCAAATTTGAACCTCAAGAGGGATATTTCTTGGTAACTGGCTCCTATGATATGACGGCAAAGATATGGTCAGCCCGAGATTTTAAGCCTGTCAAGACGCTCTCTGGACACGAATCAAAAGTTACTTCCTTGGATGTTTCAGGAGATGGACATCACATTGCTACTGTATCACATGACCGAACTATCAAACTATGGTCGAGCAGTACCAATGAGAAGGACCAGGAAATGGATGTTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0001709 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007154 GO:0007267 GO:0007275 GO:0007276 GO:0007292 GO:0008150 GO:0008152 GO:0008380 GO:0009553 GO:0009560 GO:0009561 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016604 GO:0016607 GO:0017069 GO:0017070 GO:0019953 GO:0022412 GO:0022414 GO:0023052 GO:0030154 GO:0030312 GO:0030532 GO:0030621 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044703 GO:0045165 GO:0046483 GO:0046540 GO:0048229 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0070013 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

PRP4 WD40

Protein Analysis

524

Amino Acids

57.93

Weight (kDa)

5.43

Isoelectric Point (pI)

45.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRP4	PF08799	93 - 120	5e-11	pre-mRNA processing factor 4 (PRP4) like
Beta-prop_WDR3_2nd	PF25172	221 - 345	2.2e-11	WDR3 second beta-propeller domain
WD40_Gbeta	PF25391	228 - 350	1.6e-10	G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st	PF25173	228 - 385	4.8e-23	WDR3 first beta-propeller domain
EIF3I	PF24805	228 - 303	9.4e-07	EIF3I
Beta-prop_WDR5	PF25175	230 - 413	5.7e-42	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	230 - 335	3.4e-06	WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz	PF24807	231 - 307	5.2e-08	CDC20/Fizzy WD40 domain
Beta-prop_EML_2	PF23414	231 - 349	2.6e-10	Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st	PF24817	236 - 303	3.7e-07	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	237 - 347	2.3e-19	THOC3 beta-propeller domain
WD40_Prp19	PF24814	241 - 459	2.2e-57	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	256 - 468	6.8e-11	WDR36/Utp21 first beta-propeller
WD40	PF00400	264 - 301	7.3e-08	WD domain, G-beta repeat
WDR55	PF24796	273 - 408	2.6e-11	WDR55
WD40_MABP1-WDR62_2nd	PF24782	286 - 385	6.4e-09	MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st	PF24817	286 - 349	6.6e-07	WDHD1 first WD40 domain
WD40	PF00400	306 - 342	8.8e-06	WD domain, G-beta repeat
WD40_CDC20-Fz	PF24807	308 - 510	6.5e-29	CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	309 - 389	2.1e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	320 - 452	3.1e-28	THOC3 beta-propeller domain
Beta-prop_WDR90_POC16_2nd	PF23393	323 - 414	1.4e-07	WDR90/POC16, second beta-propeller
Beta-prop_WDR3_2nd	PF25172	331 - 426	2.3e-13	WDR3 second beta-propeller domain
WD40_Gbeta	PF25391	331 - 510	6.6e-32	G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st	PF25173	340 - 511	1.8e-37	WDR3 first beta-propeller domain
WDR55	PF24796	349 - 463	1.5e-11	WDR55
EIF3I	PF24805	351 - 448	1.4e-11	EIF3I
WD40	PF00400	352 - 384	6e-08	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	353 - 516	4.7e-42	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	353 - 419	1.6e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML	PF23409	354 - 458	4.2e-10	Echinoderm microtubule-associated protein first beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	358 - 468	4.4e-08	MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st	PF24817	358 - 439	2.9e-13	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	359 - 518	3.3e-23	Echinoderm microtubule-associated protein second beta-propeller
ANAPC4_WD40	PF12894	382 - 449	9.8e-06	Anaphase-promoting complex subunit 4 WD40 domain
WD40	PF00400	389 - 426	1.7e-11	WD domain, G-beta repeat
Beta-prop_TEP1_2nd	PF25047	392 - 515	3.9e-14	TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	393 - 454	3.2e-09	CAF1B/HIR1 beta-propeller domain
WD40_MABP1-WDR62_1st	PF24780	394 - 516	1.3e-12	MABP1/WDR62 first WD40 domain
Beta-prop_EIPR1	PF23609	397 - 468	4.6e-06	EIPR1 beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	413 - 497	9.6e-07	WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	415 - 512	4.9e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	433 - 521	1.1e-15	THOC3 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	436 - 514	2.9e-15	WDR3 second beta-propeller domain
EIF3I	PF24805	438 - 517	1.1e-08	EIF3I
WD40_Prp19	PF24814	443 - 511	8.1e-12	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	446 - 520	1.2e-10	WDHD1 first WD40 domain
WD40	PF00400	474 - 511	1.3e-10	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006652)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G05720 AT2G41500 AT2G41500
fragaria_vesca	FvH4_6g24750
malus_domestica	MD12G1086300.v1.1 MD14G1080500.v1.1
prunus_persica	Prupe.7G050300_v2.0.a1 Prupe.7G050300_v2.0.a1
pyrus_communis	pycom06g09480 pycom12g07290
rosa_chinensis	RchiOBHm_Chr3g0484331 RchiOBHm_Chr3g0484401 RchiOBHm_Chr3g0484501
rosa_laevigata	RLG00000023231
rosa_multiflora	Rmu_co8232775.1_g000001 Rmu_sc0000295.1_g000017 Rmu_sc0000295.1_g000018 Rmu_sc0001947.1_g000006
rosa_roxburghii	Rroxscaffold_6G00397770
rosa_rugosa	Rorug03G0210900
rosa_samantha	Rh3AG260000 Rh3AG261700 Rh3BG296600 Rh3CG294200 Rh3DG290300
rosa_wichuraiana	Rw3G023450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	275
Acc16I	TGCGCA	1 cut(s)	543
AccB1I	GGYRCC	1 cut(s)	294
AccB7I	CCANNNNNTGG	3 cut(s)	701, 768, 1561
AccBSI	CCGCTC	1 cut(s)	134
AccII	CGCG	2 cut(s)	193, 240
AccIII	TCCGGA	2 cut(s)	279, 604
AclWI	GGATC	2 cut(s)	596, 609
AcoI	YGGCCR	2 cut(s)	366, 759
AcsI	RAATTY	2 cut(s)	474, 1340
AcuI	CTGAAG	1 cut(s)	516
AcyI	GRCGYC	1 cut(s)	295
AfaI	GTAC	6 cut(s)	85, 482, 548, 983, 991, 1540
AfeI	AGCGCT	1 cut(s)	821
AfiI	CCNNNNNNNGG	5 cut(s)	65, 701, 768, 1355, 1561
AgsI	TTSAA	5 cut(s)	578, 661, 805, 1055, 1346
AjnI	CCWGG	3 cut(s)	503, 557, 1554
AluBI	AGCT	9 cut(s)	28, 47, 223, 373, 388, 515, 664, 766, 999
AluI	AGCT	9 cut(s)	28, 47, 223, 373, 388, 515, 664, 766, 999
Alw21I	GWGCWC	2 cut(s)	442, 916
Alw26I	GTCTC	3 cut(s)	293, 386, 1025
AlwI	GGATC	2 cut(s)	596, 609
Ama87I	CYCGRG	1 cut(s)	1410
Aor13HI	TCCGGA	2 cut(s)	279, 604
Aor51HI	AGCGCT	1 cut(s)	821
AoxI	GGCC	7 cut(s)	366, 445, 506, 648, 692, 759, 1186
ApeKI	GCWGC	7 cut(s)	44, 74, 77, 217, 220, 385, 431
ApoI	RAATTY	2 cut(s)	474, 1340
ArsI	GACNNNNNNTTYG	2 cut(s)	1388, 1420
AspLEI	GCGC	9 cut(s)	193, 237, 242, 297, 385, 544, 746, 822, 1135
AspS9I	GGNCC	6 cut(s)	346, 446, 507, 943, 1150, 1552
AsuHPI	GGTGA	4 cut(s)	122, 1049, 1212, 1262
AvaI	CYCGRG	1 cut(s)	1410
AvaII	GGWCC	4 cut(s)	346, 943, 1150, 1552
AxyI	CCTNAGG	1 cut(s)	973
BalI	TGGCCA	2 cut(s)	368, 761
BamHI	GGATCC	1 cut(s)	601
BanI	GGYRCC	1 cut(s)	294
BanII	GRGCYC	1 cut(s)	57
BarI	GAAGNNNNNNTAC	4 cut(s)	473, 505, 1159, 1191
BauI	CACGAG	1 cut(s)	1140
BbsI	GAAGAC	1 cut(s)	1260
Bbv12I	GWGCWC	2 cut(s)	442, 916
BbvI	GCAGC	7 cut(s)	56, 86, 89, 229, 232, 397, 418
BccI	CCATC	5 cut(s)	328, 716, 975, 1094, 1475
BceAI	ACGGC	4 cut(s)	82, 239, 260, 1407
BcgI	CGANNNNNNTGC	2 cut(s)	410, 444
BciT130I	CCWGG	3 cut(s)	505, 559, 1556
BcoDI	GTCTC	3 cut(s)	293, 386, 1025
BfaI	CTAG	4 cut(s)	443, 528, 996, 1238
BfmI	CTRYAG	2 cut(s)	39, 1083
BfoI	RGCGCY	3 cut(s)	238, 298, 823
BmcAI	AGTACT	2 cut(s)	548, 983
Bme1390I	CCNGG	3 cut(s)	505, 559, 1556
Bme18I	GGWCC	4 cut(s)	346, 943, 1150, 1552
BmeT110I	CYCGRG	1 cut(s)	1410
BmgT120I	GGNCC	6 cut(s)	346, 446, 507, 943, 1150, 1552
BmiI	GGNNCC	8 cut(s)	106, 142, 296, 347, 448, 603, 1151, 1378
BmrFI	CCNGG	3 cut(s)	505, 559, 1556
BmrI	ACTGGG	2 cut(s)	643, 1254
BmsI	GCATC	3 cut(s)	106, 610, 1120
BmuI	ACTGGG	2 cut(s)	643, 1254
BpiI	GAAGAC	1 cut(s)	1260
BplI	GAGNNNNNCTC	4 cut(s)	635, 667, 763, 795
BpmI	CTGGAG	1 cut(s)	410
Bpu10I	CCTNAGC	1 cut(s)	353
BpuEI	CTTGAG	3 cut(s)	120, 765, 1335
BsaBI	GATNNNNATC	1 cut(s)	1485
BsaHI	GRCGYC	1 cut(s)	295
BsaJI	CCNNGG	5 cut(s)	298, 557, 558, 1179, 1464
BsaWI	WCCGGW	4 cut(s)	101, 107, 279, 604
BsaXI	ACNNNNNCTCC	2 cut(s)	83, 113
Bsc4I	CCNNNNNNNGG	5 cut(s)	65, 701, 768, 1355, 1561
Bse118I	RCCGGY	1 cut(s)	348
Bse1I	ACTGG	6 cut(s)	81, 638, 755, 1039, 1249, 1378
Bse21I	CCTNAGG	1 cut(s)	973
Bse3DI	GCAATG	3 cut(s)	219, 957, 1491
Bse8I	GATNNNNATC	1 cut(s)	1485
BseAI	TCCGGA	2 cut(s)	279, 604
BseBI	CCWGG	3 cut(s)	505, 559, 1556
BseDI	CCNNGG	5 cut(s)	298, 557, 558, 1179, 1464
BseGI	GGATG	8 cut(s)	613, 625, 691, 967, 1030, 1135, 1474, 1570
BseJI	GATNNNNATC	1 cut(s)	1485
BseLI	CCNNNNNNNGG	5 cut(s)	65, 701, 768, 1355, 1561
BseMI	GCAATG	3 cut(s)	219, 957, 1491
BseMII	CTCAG	3 cut(s)	367, 987, 1225
BseNI	ACTGG	6 cut(s)	81, 638, 755, 1039, 1249, 1378
BseRI	GAGGAG	8 cut(s)	25, 26, 29, 32, 35, 136, 601, 611
BseXI	GCAGC	7 cut(s)	56, 86, 89, 229, 232, 397, 418
Bsh1236I	CGCG	2 cut(s)	193, 240
Bsh1285I	CGRYCG	1 cut(s)	90
BshFI	GGCC	7 cut(s)	368, 447, 508, 650, 694, 761, 1188
BshNI	GGYRCC	1 cut(s)	294
BsiEI	CGRYCG	1 cut(s)	90
BsiHKAI	GWGCWC	2 cut(s)	442, 916
BsiHKCI	CYCGRG	1 cut(s)	1410
BsiSI	CCGG	7 cut(s)	102, 108, 153, 280, 292, 349, 605
BslFI	GGGAC	5 cut(s)	242, 359, 822, 948, 1163
BslI	CCNNNNNNNGG	5 cut(s)	65, 701, 768, 1355, 1561
BsmAI	GTCTC	3 cut(s)	293, 386, 1025
BsmBI	CGTCTC	2 cut(s)	293, 386
BsmFI	GGGAC	5 cut(s)	242, 359, 822, 948, 1163
BsnI	GGCC	7 cut(s)	368, 447, 508, 650, 694, 761, 1188
BsoBI	CYCGRG	1 cut(s)	1410
Bsp1286I	GDGCHC	3 cut(s)	57, 442, 916
Bsp13I	TCCGGA	2 cut(s)	279, 604
Bsp143I	GATC	3 cut(s)	466, 601, 627
BspANI	GGCC	7 cut(s)	368, 447, 508, 650, 694, 761, 1188
BspCNI	CTCAG	3 cut(s)	366, 986, 1224
BspEI	TCCGGA	2 cut(s)	279, 604
BspFNI	CGCG	2 cut(s)	193, 240
BspLI	GGNNCC	8 cut(s)	106, 142, 296, 347, 448, 603, 1151, 1378
BspPI	GGATC	2 cut(s)	596, 609
BspQI	GCTCTTC	1 cut(s)	171
BspT107I	GGYRCC	1 cut(s)	294
BsrBI	CCGCTC	1 cut(s)	134
BsrDI	GCAATG	3 cut(s)	219, 957, 1491
BsrFI	RCCGGY	1 cut(s)	348
BsrI	ACTGG	6 cut(s)	81, 638, 755, 1039, 1249, 1378
BssAI	RCCGGY	1 cut(s)	348
BssECI	CCNNGG	5 cut(s)	298, 557, 558, 1179, 1464
BssMI	GATC	3 cut(s)	466, 601, 627
BssNI	GRCGYC	1 cut(s)	295
BssSI	CACGAG	1 cut(s)	1140
BssT1I	CCWWGG	2 cut(s)	1179, 1464
Bst2BI	CACGAG	1 cut(s)	1140
Bst2UI	CCWGG	3 cut(s)	505, 559, 1556
Bst4CI	ACNGT	3 cut(s)	947, 1067, 1501
Bst6I	CTCTTC	2 cut(s)	171, 1035
BstACI	GRCGYC	1 cut(s)	295
BstC8I	GCNNGC	7 cut(s)	305, 648, 735, 742, 776, 955, 1137
BstDEI	CTNAG	3 cut(s)	353, 973, 1211
BstF5I	GGATG	8 cut(s)	613, 625, 691, 967, 1030, 1135, 1474, 1570
BstFNI	CGCG	2 cut(s)	193, 240
BstH2I	RGCGCY	3 cut(s)	238, 298, 823
BstHHI	GCGC	9 cut(s)	193, 237, 242, 297, 385, 544, 746, 822, 1135
BstKTI	GATC	3 cut(s)	469, 604, 630
BstMAI	GTCTC	3 cut(s)	293, 386, 1025
BstMBI	GATC	3 cut(s)	466, 601, 627
BstMCI	CGRYCG	1 cut(s)	90
BstNI	CCWGG	3 cut(s)	505, 559, 1556
BstNSI	RCATGY	1 cut(s)	778
BstSCI	CCNGG	3 cut(s)	503, 557, 1554
BstSFI	CTRYAG	2 cut(s)	39, 1083
BstUI	CGCG	2 cut(s)	193, 240
BstV1I	GCAGC	7 cut(s)	56, 86, 89, 229, 232, 397, 418
BstV2I	GAAGAC	1 cut(s)	1260
BstX2I	RGATCY	1 cut(s)	601
BstXI	CCANNNNNNTGG	1 cut(s)	1100
BstYI	RGATCY	1 cut(s)	601
Bsu36I	CCTNAGG	1 cut(s)	973
BsuRI	GGCC	7 cut(s)	368, 447, 508, 650, 694, 761, 1188
BtsCI	GGATG	8 cut(s)	613, 625, 691, 967, 1030, 1135, 1474, 1570
BtsI	GCAGTG	1 cut(s)	679
BtsIMutI	CAGTG	4 cut(s)	492, 679, 762, 1242
Cac8I	GCNNGC	7 cut(s)	305, 648, 735, 742, 776, 955, 1137
CfoI	GCGC	9 cut(s)	193, 237, 242, 297, 385, 544, 746, 822, 1135
Cfr10I	RCCGGY	1 cut(s)	348
Cfr13I	GGNCC	6 cut(s)	346, 446, 507, 943, 1150, 1552
CseI	GACGC	4 cut(s)	403, 533, 861, 1441
Csp6I	GTAC	6 cut(s)	84, 481, 547, 982, 990, 1539
CviAII	CATG	3 cut(s)	775, 1190, 1508
CviQI	GTAC	6 cut(s)	84, 481, 547, 982, 990, 1539
DdeI	CTNAG	3 cut(s)	353, 973, 1211
DinI	GGCGCC	1 cut(s)	296
DpnI	GATC	3 cut(s)	468, 603, 629
DpnII	GATC	3 cut(s)	466, 601, 627
DrdI	GACNNNNNNGTC	1 cut(s)	275
DseDI	GACNNNNNNGTC	1 cut(s)	275
EaeI	YGGCCR	2 cut(s)	366, 759
Eam1104I	CTCTTC	2 cut(s)	171, 1035
EarI	CTCTTC	2 cut(s)	171, 1035
EciI	GGCGGA	2 cut(s)	139, 235
Eco130I	CCWWGG	2 cut(s)	1179, 1464
Eco147I	AGGCCT	1 cut(s)	650
Eco24I	GRGCYC	1 cut(s)	57
Eco47I	GGWCC	4 cut(s)	346, 943, 1150, 1552
Eco47III	AGCGCT	1 cut(s)	821
Eco57I	CTGAAG	1 cut(s)	516
Eco81I	CCTNAGG	1 cut(s)	973
Eco88I	CYCGRG	1 cut(s)	1410
EcoO109I	RGGNCCY	2 cut(s)	446, 1150
EcoRII	CCWGG	3 cut(s)	503, 557, 1554
EcoT14I	CCWWGG	2 cut(s)	1179, 1464
EcoT38I	GRGCYC	1 cut(s)	57
EgeI	GGCGCC	1 cut(s)	296
EheI	GGCGCC	1 cut(s)	296
ErhI	CCWWGG	2 cut(s)	1179, 1464
Esp3I	CGTCTC	2 cut(s)	293, 386
FaeI	CATG	3 cut(s)	778, 1193, 1511
FalI	AAGNNNNNCTT	2 cut(s)	1032, 1064
FaqI	GGGAC	5 cut(s)	242, 359, 822, 948, 1163
FatI	CATG	3 cut(s)	774, 1189, 1507
FauI	CCCGC	2 cut(s)	336, 962
FokI	GGATG	7 cut(s)	620, 632, 698, 954, 1037, 1142, 1481
FriOI	GRGCYC	1 cut(s)	57
FspBI	CTAG	4 cut(s)	443, 528, 996, 1238
FspI	TGCGCA	1 cut(s)	543
GlaI	GCGC	9 cut(s)	192, 236, 241, 296, 384, 543, 745, 821, 1134
GsuI	CTGGAG	1 cut(s)	410
HaeII	RGCGCY	3 cut(s)	238, 298, 823
HaeIII	GGCC	7 cut(s)	368, 447, 508, 650, 694, 761, 1188
HapII	CCGG	7 cut(s)	102, 108, 153, 280, 292, 349, 605
HgaI	GACGC	4 cut(s)	403, 533, 861, 1441
HhaI	GCGC	9 cut(s)	193, 237, 242, 297, 385, 544, 746, 822, 1135
Hin1I	GRCGYC	1 cut(s)	295
Hin1II	CATG	3 cut(s)	778, 1193, 1511
Hin6I	GCGC	9 cut(s)	191, 235, 240, 295, 383, 542, 744, 820, 1133
HinP1I	GCGC	9 cut(s)	191, 235, 240, 295, 383, 542, 744, 820, 1133
HindIII	AAGCTT	2 cut(s)	26, 662
HinfI	GANTC	9 cut(s)	92, 98, 156, 283, 313, 361, 377, 455, 1448
HpaII	CCGG	7 cut(s)	102, 108, 153, 280, 292, 349, 605
HphI	GGTGA	4 cut(s)	122, 1049, 1212, 1262
Hpy188I	TCNGA	5 cut(s)	52, 97, 175, 323, 460
Hpy99I	CGWCG	2 cut(s)	416, 455
HpyAV	CCTTC	9 cut(s)	397, 496, 586, 799, 902, 973, 1052, 1178, 1543
HpyCH4III	ACNGT	3 cut(s)	947, 1067, 1501
HpyCH4IV	ACGT	1 cut(s)	580
HpyCH4V	TGCA	6 cut(s)	217, 672, 836, 850, 1115, 1208
HpyF3I	CTNAG	3 cut(s)	353, 973, 1211
HpySE526I	ACGT	1 cut(s)	580
Hsp92I	GRCGYC	1 cut(s)	295
Hsp92II	CATG	3 cut(s)	778, 1193, 1511
HspAI	GCGC	9 cut(s)	191, 235, 240, 295, 383, 542, 744, 820, 1133
KasI	GGCGCC	1 cut(s)	294
Kpn2I	TCCGGA	2 cut(s)	279, 604
Kzo9I	GATC	3 cut(s)	466, 601, 627
LguI	GCTCTTC	1 cut(s)	171
LmnI	GCTCC	4 cut(s)	140, 771, 911, 1382
Lsp1109I	GCAGC	7 cut(s)	56, 86, 89, 229, 232, 397, 418
LweI	GCATC	3 cut(s)	106, 610, 1120
MaeI	CTAG	4 cut(s)	443, 528, 996, 1238
MaeII	ACGT	1 cut(s)	580
MaeIII	GTNAC	6 cut(s)	110, 521, 784, 1061, 1369, 1456
MalI	GATC	3 cut(s)	468, 603, 629
MbiI	CCGCTC	1 cut(s)	134
MboI	GATC	3 cut(s)	466, 601, 627
MboII	GAAGA	5 cut(s)	188, 481, 1043, 1052, 1265
MflI	RGATCY	1 cut(s)	601
MhlI	GDGCHC	3 cut(s)	57, 442, 916
MlsI	TGGCCA	2 cut(s)	368, 761
MluCI	AATT	4 cut(s)	474, 655, 728, 1340
MluNI	TGGCCA	2 cut(s)	368, 761
Mly113I	GGCGCC	1 cut(s)	295
MlyI	GAGTC	5 cut(s)	86, 92, 165, 277, 307
MmeI	TCCRAC	1 cut(s)	120
Mox20I	TGGCCA	2 cut(s)	368, 761
MroI	TCCGGA	2 cut(s)	279, 604
MscI	TGGCCA	2 cut(s)	368, 761
MseI	TTAA	4 cut(s)	749, 824, 1280, 1419
MslI	CAYNNNNRTG	1 cut(s)	1098
Msp20I	TGGCCA	2 cut(s)	368, 761
MspA1I	CMGCKG	1 cut(s)	388
MspI	CCGG	7 cut(s)	102, 108, 153, 280, 292, 349, 605
MspR9I	CCNGG	3 cut(s)	505, 559, 1556
MteI	GCGCNGCGC	1 cut(s)	238
MvaI	CCWGG	3 cut(s)	505, 559, 1556
MvnI	CGCG	2 cut(s)	193, 240
NarI	GGCGCC	1 cut(s)	295
NdeII	GATC	3 cut(s)	466, 601, 627
NlaIII	CATG	3 cut(s)	778, 1193, 1511
NlaIV	GGNNCC	8 cut(s)	106, 142, 296, 347, 448, 603, 1151, 1378
NmeAIII	GCCGAG	2 cut(s)	279, 649
NmuCI	GTSAC	3 cut(s)	110, 521, 1061
NsbI	TGCGCA	1 cut(s)	543
NspI	RCATGY	1 cut(s)	778
PaeI	GCATGC	1 cut(s)	778
PasI	CCCWGGG	1 cut(s)	558
PceI	AGGCCT	1 cut(s)	650
PciSI	GCTCTTC	1 cut(s)	171
PcsI	WCGNNNNNNNCGW	2 cut(s)	273, 417
PfeI	GAWTC	4 cut(s)	361, 377, 455, 1448
PflMI	CCANNNNNTGG	3 cut(s)	701, 768, 1561
PleI	GAGTC	5 cut(s)	86, 92, 164, 277, 307
PluTI	GGCGCC	1 cut(s)	298
PpsI	GAGTC	5 cut(s)	86, 92, 164, 277, 307
PpuMI	RGGWCCY	1 cut(s)	1150
Psp5II	RGGWCCY	1 cut(s)	1150
Psp6I	CCWGG	3 cut(s)	503, 557, 1554
PspGI	CCWGG	3 cut(s)	503, 557, 1554
PspN4I	GGNNCC	8 cut(s)	106, 142, 296, 347, 448, 603, 1151, 1378
PspPI	GGNCC	6 cut(s)	346, 446, 507, 943, 1150, 1552
PspPPI	RGGWCCY	1 cut(s)	1150
PsuI	RGATCY	1 cut(s)	601
PvuII	CAGCTG	1 cut(s)	388
RsaI	GTAC	6 cut(s)	85, 482, 548, 983, 991, 1540
RsaNI	GTAC	6 cut(s)	84, 481, 547, 982, 990, 1539
RseI	CAYNNNNRTG	1 cut(s)	1098
SapI	GCTCTTC	1 cut(s)	171
SaqAI	TTAA	4 cut(s)	749, 824, 1280, 1419
Sau3AI	GATC	3 cut(s)	466, 601, 627
Sau96I	GGNCC	6 cut(s)	346, 446, 507, 943, 1150, 1552
ScaI	AGTACT	2 cut(s)	548, 983
SchI	GAGTC	5 cut(s)	86, 92, 165, 277, 307
ScrFI	CCNGG	3 cut(s)	505, 559, 1556
SduI	GDGCHC	3 cut(s)	57, 442, 916
SfaNI	GCATC	3 cut(s)	106, 610, 1120
SfcI	CTRYAG	2 cut(s)	39, 1083
SfoI	GGCGCC	1 cut(s)	296
SinI	GGWCC	4 cut(s)	346, 943, 1150, 1552
SmiMI	CAYNNNNRTG	1 cut(s)	1098
SmlI	CTYRAG	3 cut(s)	135, 780, 1350
SmoI	CTYRAG	3 cut(s)	135, 780, 1350
SphI	GCATGC	1 cut(s)	778
Sse9I	AATT	4 cut(s)	474, 655, 728, 1340
SseBI	AGGCCT	1 cut(s)	650
SspDI	GGCGCC	1 cut(s)	294
SspMI	CTAG	4 cut(s)	443, 528, 996, 1238
StuI	AGGCCT	1 cut(s)	650
StyD4I	CCNGG	3 cut(s)	503, 557, 1554
StyI	CCWWGG	2 cut(s)	1179, 1464
TaaI	ACNGT	3 cut(s)	947, 1067, 1501
TaiI	ACGT	1 cut(s)	583
TaqI	TCGA	5 cut(s)	159, 359, 375, 465, 1532
TaqII	GACCGA	2 cut(s)	104, 1527
TasI	AATT	4 cut(s)	474, 655, 728, 1340
TatI	WGTACW	2 cut(s)	546, 981
TfiI	GAWTC	4 cut(s)	361, 377, 455, 1448
Tru1I	TTAA	4 cut(s)	749, 824, 1280, 1419
Tru9I	TTAA	4 cut(s)	749, 824, 1280, 1419
TscAI	CASTG	4 cut(s)	499, 679, 762, 1249
TseFI	GTSAC	3 cut(s)	110, 521, 1061
TseI	GCWGC	7 cut(s)	44, 74, 77, 217, 220, 385, 431
Tsp45I	GTSAC	3 cut(s)	110, 521, 1061
TspDTI	ATGAA	3 cut(s)	245, 416, 524
TspGWI	ACGGA	1 cut(s)	1145
TspRI	CASTG	4 cut(s)	499, 679, 762, 1249
Van91I	CCANNNNNTGG	3 cut(s)	701, 768, 1561
VpaK11BI	GGWCC	4 cut(s)	346, 943, 1150, 1552
XapI	RAATTY	2 cut(s)	474, 1340
XceI	RCATGY	1 cut(s)	778
XspI	CTAG	4 cut(s)	443, 528, 996, 1238
ZrmI	AGTACT	2 cut(s)	548, 983

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.