MD12G1103600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008872
Physical Location & Seq
Forward (+)
16351184 .. 16358368
7185 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1103600.v1.1.491

Sequence Viewer

Length: 4383 bp
ATGTTCAGGGTGCACAAGAACAGGCCTGCTAAGTCAGGGGAGAGGGTTGATTTCAAGTTCTCCCACCTTAAGGCCCTCCAGGTCCCCAGAGGATGGGACAAGCTGTTTGTGTCTATCGTCTCTGTAGAAACTGGGAAACCAATTGCGAAATCAAGCAAAGCAGTGGTGCGCAACGGAAACTGTCAATGGACTGAGACTCTCTGCGAATCTATATGGATTTCCCAAGATGAGTCGTCCAAGGAGATGGAAGATTATTTCATCAAGCTTGTTATTTCAATGGGATCAGCAAGATCCGGTATCCTTGGAGAGGTTTCAGTCAATATGTCGGATTACATAAGTTCAACCTCTTCTGCTCCAGTTTCTCTTCCATTGAAAAAATGCAGTTTTGGGACAATTCTGCAGGTAAAAATTAACTGCCTCACACCAAGAAAAAGGCTCAGTGATGAAGAGTCAAAAGAGACAAGGGATCATTTGGAAGAAGCAAATGCAAATGGTCATGATGCAGACAGCAAGTCAGATGGATCTAATACCTCATTTGTACAGAGTGCTGGATCTTCCTTCGGAAAAGATTTTGGCTTGATCTCAAATCCTGGAGAGCCTGGGAGTCGAGGTAGCAGTTTCTCTGCATCAGGTTCACATCACAGCTATGACTCAGCGGAGGGCTCCATAAGAAGGGATAATATGTCCCCGAGAAGCAACCTTAGTGTTGAAGGCAACCACCTGATAGGAAGACAAGATTCAATCACCTCCCTAATTAGTACAACTCATGGAAACTTTCCTGCTGATATCCCTTCTCCATCAAATAATTCATCATTTAATTCCAGGATTAATGGTTCAGGAAATCATTCTCAGAATTCTCGGAAAGATTTTAAAGAATCATCTTTAAAAGCCACTGATTCTTCTAAAAACCTTCTGGACGCGGCAGAAGTTACGATTGAAGAATTACATGCGGAAGCTAAGATGTGGGAGAGGAACGCCAGGAAGCTAATGCTTGATTTGGATATACTGAGGACAGAATTCTCTGATCAGTCCAAAAAGCAGGCAAATATGAAAGTGGAGCTTTCAGCAGCATATGCAGAACGCAATGGCTTGAAGAAAGAAGTTGAACACTTGCAATTGTTGCTTGAGAATTCAGTGGTTAAACAAACTGCCAGTGAGAATTTAACTTATCAGGATGAAGTAACGCCAGGGATTGAGAAGGCGTTGCAAGATGAGCTGAAGTTTCAGAAAGAATCTGTTGCCAATCTGGCTCTGCAGTTGGAGAGAAGTCAAGAATCAAATATTGAGCTGGTTTCGGTTCTCCAGGAGCTGGAAGAGACAATTGAAAACCAAAAGGTGGAGTTGGAGAATCTTTCAGAACTACAGTCAACATTTGGTGATATGGAAAATTTGATCAAATTAACCAGAGAGGAAAACAGAAATTTAAAGCTTCAGCTGCAACAATTGCAGGAATCAGAGAACAAGTTGCAAGTTGCGGTGCAACAGTTTGAACAAGCTGTGGAAGAGAAAAACCATGAGATAGAGAATGGATCAAGTCTGAACGAACACTCCGTTTTGGACATTGAGACAGAATACAAATGTAAACTGCTTTTGAAAGAACAAGAAATTGTTAAACTGAAAGCAAAGGTATCTGAGTCTCTCAAAGAAAGGCATTCTACAGAAATGGATTCCATCACTGTGAGTGGGGGTGAAGCAGATCTGATCAGAGAAATTGCGGACTTGAAAGAAAAGGTGCAGGAGCTAGAAAGAGATTGCAACGAGCTGACAGAAGAAAACTTAGAGCTCTTGTTCAAGCTTAAGGCATCGAAGAAAAAATCTTCAGGGGGACACGCACCTGGTGAACTTCCATCCAGTGAGCTTTTATTCGATTCTTTTACCAGCTTTGAGTCCGAAGTAACTGAAAATAAACACCAAATACAAAATGTGGAGGAGAAGCTTAACAAGAAAGTGCTTGGGGAGATTACAAATAATAATGATGTTTCAGTTCAAGTACATGACAGCTTGAAAATGGAACTAGAAAGTAAAGTCACAGAGCTGGGTAAAGAATTGACAGAGAAGAGGTTCGAAATAGAAAAACTTGAGGCAAACTTGCTAACCAATGAAGAAGAAATCAGTCTTCTAAAAGGGGTCCAGAATGAATTAGAAGCTAAGGTTTCCGATCTTCAGAAGGAAAAAATACAGCTAGAGGAACACATGGAAGTTGTATTAAGGGAAAGCGATATCAGCTCTAAGTGTTTGAATGATTTGCGGCATGAATTAATGGTGCTTAGCAGAAGTGTGAATTCCCATGTTTCCACACATAGGGTACTTGAAAGGAAATCTTCGGAGCTAGAAGCTGATAAATGTGAACTGGACCGTCACATATCAGAGCTTGAACAAGAAAACATACAGTTGTCAGCAAGTGGTTCTGCACTCGAAGCTCAAATAAGATGTTTGACAGATGAAAAAGAGGCCAGTCAATTAGAATTAGAGAATTCCCAATCTTATTCTCTGAGTCTCCAAGATGAGATCAGTAGATTGAAAACTGAGATGGAGTCTGATAAAGTTGAACTGAAACAGAAATTAAAGGACTTGGAGAGTCAGTGGTCAGAAGCTCGAGATGAATGTGAATTTCTGAAAAGGGCAAACCCGGAGCTACAAGCTACTGCTGAGAGCCTCATTGAAGAGTGCAATTCTCTTCAGACTTCAAATGATGAGTTGAGGAAACAAAAGCTGGAGTTGCATCAGCTCTGTTCCGTCTTGGAGGCAAAGTTGAATCAATCGCACGAGAGCTTTACAAATTGCTCCAAAAGAGTTGAAGTGCTGGAAAAGGATCTTTCTTTAATGCTGGAAAACATCGCCTCAAAAGAGGAGAGCCTGAATTTAGAATTAGATGCACTTCTTGATGAAAACATGAAATATAAGGAGAAGCTTACATTGGAAGAAAGTTTGTTCAACAAGATGTATTTGGAGAAGACGGTTGAAGTTGAGAGCCTTCAGGAAGAGGTAGATCGACTTACCAAGCTACTTGCTGCAACTCAAGAAGAAGGAGAAGAACTAGCTTCAGATGCCGTCCAGGAAGCATCCAGATTACGAACTGAGAAGGAAATGCTTGAGTCTGCTCTTCGAGAGGTTCAATCTAAAGCAATACAGACTGAGGAAGAATTGAATATTATGAGAATAGAAACTGAAGCGAAGCTACAAGGCCTGGTCACTGAGCTTGCTGCCTCTAAACAAAACCAGGAAGTAATGATGGCTGACCATGAAAGGCTATTGAAGTTGTTGGAGAATTACAAATCGAGTGAAGCAAAACTGAAGACCACTGTCAATGACCTTGAACTAAAGCTTACAGTTTCTGATTATGAACGTCACCAAGTGTTGGAAGAATCTATTAATATGAAGGTTCAGATTGAGAAACTAACTCATTGTCAGGAGGAAGTTTTGGCTTCCAACAATGAGCTGGATGCAACCAAGTTTGAGAAAGAAAAATTGGAAGTGTTACTGCATTCAATTTCTGAGGAATGTCAAGATCTGAAGGCAGAGAAAAGTTCATTCCATGAGAAGATCTCAACATTGGAGAACGCCTTATTTGAATTGGAGGATTGCAAACGTAATAATGTTCTCTTGGAGGAAAAAGTTCTGCAGATGGAAGGTGATCTAACTGCAAAAGAGGCTTTGTGTGCACAGTATGCCGAGCTGAAGAACGAGCTCAACCAGATCAGGAGAGCCAACGAGCAGTATCAGCTGAAAATACAGCTACTTGAGGAGGAGAGAAGTGAGTTCCTAAGAAAGTCTCAGGCCCTTGAACAAGAATTGAAACTAACAAGGGAAGAAAAGCAGAAACAGAGGGATTCCAGCAGTCCAAAGATTTCCAGCTCCTCAAAAGGCGGCACCAGAGCTATTCCTGTTGGTGAAGATATGAAGGTCAACGAAATGGGGAAGAACAGCATTCCCCGTCGTGATTTTAGAAGGAAGCCAGTTTTGAAGAATGGTCAGGTGCAAGGACATGCAAGAGATCAGAATTATCCATATAGAAACCAGCATCAAAGAGAGGATGACATTGGAATCCAATTTCATGATGAAAATCCAAATTACGTTGAAGAACTTGGCTTAAAGATTAAGTTGCTTGAAGATGAACTTGTTAAGGCATTGGAAGCAAATAACACTTACAAAGTTCAGCTTGATAGGTACTTGTCTGAGGCCCGACACAGTCAAGCAGATGCTCGTCGAAATTCAAAAGCTGAAGAAAGACACGAACGCTCGAGGTCATCTCTAGAGGCAGAACTGAAAGACATTCGCGAGCGCTACTTAGACATGAGCCTGAGATACGCGGAGGTAGAAGCTCAGCGCGAGGAACTTGTGATGAAGCTCAAAACAGCCAAGGGTGGCAAAAGGTGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1461

Amino Acids

166.35

Weight (kDa)

5.06

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 16 - 140 3.5e-13 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 170
Acc36I ACCTGC 1 cut(s) 391
AccB1I GGYRCC 1 cut(s) 3869
AccB7I CCANNNNNTGG 4 cut(s) 93, 1309, 1336, 3358
AccII CGCG 4 cut(s) 920, 4278, 4310, 4329
AciI CCGC 8 cut(s) 656, 920, 950, 1475, 1715, 2248, 3867, 4310
AclWI GGATC 7 cut(s) 285, 289, 474, 529, 559, 1537, 2820
AdeI CACNNNGTG 2 cut(s) 1838, 3355
AfaI GTAC 5 cut(s) 540, 760, 1992, 2307, 4169
AfeI AGCGCT 1 cut(s) 4283
AfiI CCNNNNNNNGG 8 cut(s) 70, 93, 307, 672, 1309, 1336, 2301, 3358
AflII CTTAAG 2 cut(s) 68, 1796
AhdI GACNNNNNGTC 1 cut(s) 511
AjuI GAANNNNNNNTTGG 4 cut(s) 2900, 2932, 3452, 3484
AloI GAACNNNNNNTCC 4 cut(s) 817, 849, 1532, 1564
Alw21I GWGCWC 4 cut(s) 15, 1785, 3664, 3690
Alw26I GTCTC 8 cut(s) 124, 188, 452, 1310, 1559, 1641, 2501, 3777
Alw44I GTGCAC 2 cut(s) 11, 3660
AlwI GGATC 7 cut(s) 285, 289, 474, 529, 559, 1537, 2820
AlwNI CAGNNNCTG 2 cut(s) 1309, 3335
Ama87I CYCGRG 3 cut(s) 688, 2595, 4240
Aor51HI AGCGCT 1 cut(s) 4283
AoxI GGCC 6 cut(s) 23, 72, 2451, 3184, 3777, 4179
ApaLI GTGCAC 2 cut(s) 11, 3660
ApeKI GCWGC 4 cut(s) 1067, 1435, 3011, 3203
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
AseI ATTAAT 3 cut(s) 828, 2258, 3372
Asp700I GAANNNNTTC 3 cut(s) 844, 2060, 3615
AspLEI GCGC 3 cut(s) 171, 4284, 4329
AspS9I GGNCC 6 cut(s) 73, 82, 2128, 2353, 3778, 4180
AsuC2I CCSGG 1 cut(s) 2630
AsuHPI GGTGA 7 cut(s) 736, 1388, 1700, 1850, 3341, 3644, 3902
AsuII TTCGAA 1 cut(s) 2064
AvaI CYCGRG 3 cut(s) 688, 2595, 4240
AvaII GGWCC 3 cut(s) 82, 2128, 2353
BaeGI GKGCMC 2 cut(s) 15, 3664
BanI GGYRCC 1 cut(s) 3869
BanII GRGCYC 3 cut(s) 665, 1785, 3690
BarI GAAGNNNNNNTAC 2 cut(s) 3162, 3194
BauI CACGAG 1 cut(s) 2765
BbsI GAAGAC 4 cut(s) 736, 2108, 2960, 3302
Bbv12I GWGCWC 4 cut(s) 15, 1785, 3664, 3690
BbvI GCAGC 4 cut(s) 1079, 1422, 2998, 3190
BccI CCATC 9 cut(s) 87, 238, 512, 805, 1679, 1855, 2524, 3226, 3619
BceAI ACGGC 1 cut(s) 3035
BciVI GTATCC 1 cut(s) 308
BclI TGATCA 3 cut(s) 1024, 1392, 1701
BcnI CCSGG 1 cut(s) 2630
BcoDI GTCTC 8 cut(s) 124, 188, 452, 1310, 1559, 1641, 2501, 3777
BfaI CTAG 6 cut(s) 1742, 2015, 2183, 2330, 3038, 4253
BfmI CTRYAG 6 cut(s) 123, 398, 1253, 1361, 1656, 3620
BfoI RGCGCY 1 cut(s) 4285
BfrI CTTAAG 2 cut(s) 68, 1796
BfuAI ACCTGC 1 cut(s) 391
BfuI GTATCC 1 cut(s) 308
BglI GCCNNNNNGGC 1 cut(s) 1247
BglII AGATCT 3 cut(s) 1696, 3508, 3543
BisI GCNGC 7 cut(s) 921, 1068, 1436, 2249, 3012, 3204, 3868
BlpI GCTNAGC 2 cut(s) 2267, 4323
BlsI GCNGC 7 cut(s) 922, 1069, 1437, 2250, 3013, 3205, 3869
Bme18I GGWCC 3 cut(s) 82, 2128, 2353
BmeRI GACNNNNNGTC 1 cut(s) 511
BmeT110I CYCGRG 3 cut(s) 688, 2595, 4240
BmgT120I GGNCC 6 cut(s) 73, 82, 2128, 2353, 3778, 4180
BmiI GGNNCC 4 cut(s) 84, 664, 2129, 3871
BmrI ACTGGG 1 cut(s) 141
BmuI ACTGGG 1 cut(s) 141
BoxI GACNNNNGTC 1 cut(s) 3302
BpiI GAAGAC 4 cut(s) 736, 2108, 2960, 3302
BplI GAGNNNNNCTC 4 cut(s) 3530, 3562, 4234, 4266
BpmI CTGGAG 5 cut(s) 62, 339, 612, 1286, 2735
Bpu10I CCTNAGC 1 cut(s) 2148
Bpu1102I GCTNAGC 2 cut(s) 2267, 4323
Bpu14I TTCGAA 1 cut(s) 2064
BpuEI CTTGAG 5 cut(s) 1145, 2099, 3003, 3113, 3761
BpuMI CCSGG 1 cut(s) 2630
BsaBI GATNNNNATC 1 cut(s) 4062
BsaJI CCNNGG 5 cut(s) 237, 301, 599, 1187, 4359
BsaWI WCCGGW 1 cut(s) 293
BsaXI ACNNNNNCTCC 8 cut(s) 595, 625, 1532, 1562, 2567, 2597, 2735, 2765
Bsc4I CCNNNNNNNGG 8 cut(s) 70, 93, 307, 672, 1309, 1336, 2301, 3358
Bse1I ACTGG 7 cut(s) 136, 356, 1152, 1851, 2355, 2454, 3956
Bse3DI GCAATG 1 cut(s) 1090
Bse8I GATNNNNATC 1 cut(s) 4062
BseDI CCNNGG 5 cut(s) 237, 301, 599, 1187, 4359
BseGI GGATG 6 cut(s) 98, 1180, 1847, 3062, 3448, 4039
BseJI GATNNNNATC 1 cut(s) 4062
BseLI CCNNNNNNNGG 8 cut(s) 70, 93, 307, 672, 1309, 1336, 2301, 3358
BseMI GCAATG 1 cut(s) 1090
BseNI ACTGG 7 cut(s) 136, 356, 1152, 1851, 2355, 2454, 3956
BseRI GAGGAG 5 cut(s) 1943, 2864, 3758, 3761, 3847
BseSI GKGCMC 2 cut(s) 15, 3664
BseXI GCAGC 4 cut(s) 1079, 1422, 2998, 3190
BseYI CCCAGC 1 cut(s) 2035
BsgI GTGCAG 2 cut(s) 1754, 2394
Bsh1236I CGCG 4 cut(s) 920, 4278, 4310, 4329
BshFI GGCC 6 cut(s) 25, 74, 2453, 3186, 3779, 4181
BshNI GGYRCC 1 cut(s) 3869
BsiHKAI GWGCWC 4 cut(s) 15, 1785, 3664, 3690
BsiHKCI CYCGRG 3 cut(s) 688, 2595, 4240
BsiSI CCGG 2 cut(s) 294, 2630
BslFI GGGAC 5 cut(s) 68, 110, 403, 670, 1839
BslI CCNNNNNNNGG 8 cut(s) 70, 93, 307, 672, 1309, 1336, 2301, 3358
BsmAI GTCTC 8 cut(s) 124, 188, 452, 1310, 1559, 1641, 2501, 3777
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 5 cut(s) 68, 110, 403, 670, 1839
BsmI GAATGC 3 cut(s) 1651, 3484, 3927
BsnI GGCC 6 cut(s) 25, 74, 2453, 3186, 3779, 4181
BsoBI CYCGRG 3 cut(s) 688, 2595, 4240
Bsp119I TTCGAA 1 cut(s) 2064
Bsp1286I GDGCHC 5 cut(s) 15, 665, 1785, 3664, 3690
Bsp1407I TGTACA 1 cut(s) 538
Bsp1720I GCTNAGC 2 cut(s) 2267, 4323
Bsp68I TCGCGA 1 cut(s) 4278
BspACI CCGC 8 cut(s) 656, 920, 950, 1475, 1715, 2248, 3867, 4310
BspANI GGCC 6 cut(s) 25, 74, 2453, 3186, 3779, 4181
BspFNI CGCG 4 cut(s) 920, 4278, 4310, 4329
BspHI TCATGA 2 cut(s) 496, 4054
BspLI GGNNCC 4 cut(s) 84, 664, 2129, 3871
BspMAI CTGCAG 3 cut(s) 402, 1257, 3624
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 7 cut(s) 285, 289, 474, 529, 559, 1537, 2820
BspQI GCTCTTC 1 cut(s) 3108
BspT104I TTCGAA 1 cut(s) 2064
BspT107I GGYRCC 1 cut(s) 3869
BspTI CTTAAG 2 cut(s) 68, 1796
BsrDI GCAATG 1 cut(s) 1090
BsrGI TGTACA 1 cut(s) 538
BsrI ACTGG 7 cut(s) 136, 356, 1152, 1851, 2355, 2454, 3956
BssECI CCNNGG 5 cut(s) 237, 301, 599, 1187, 4359
BssSI CACGAG 1 cut(s) 2765
BssT1I CCWWGG 3 cut(s) 237, 301, 4359
Bst2BI CACGAG 1 cut(s) 2765
BstAFI CTTAAG 2 cut(s) 68, 1796
BstAPI GCANNNNNTGC 4 cut(s) 1073, 1120, 1444, 3668
BstAUI TGTACA 1 cut(s) 538
BstBI TTCGAA 1 cut(s) 2064
BstC8I GCNNGC 4 cut(s) 27, 1041, 3201, 4280
BstENI CCTNNNNNAGG 1 cut(s) 305
BstF5I GGATG 6 cut(s) 98, 1180, 1847, 3062, 3448, 4039
BstFNI CGCG 4 cut(s) 920, 4278, 4310, 4329
BstH2I RGCGCY 1 cut(s) 4285
BstHHI GCGC 3 cut(s) 171, 4284, 4329
BstMAI GTCTC 8 cut(s) 124, 188, 452, 1310, 1559, 1641, 2501, 3777
BstNSI RCATGY 2 cut(s) 950, 3989
BstPAI GACNNNNGTC 1 cut(s) 3302
BstSFI CTRYAG 6 cut(s) 123, 398, 1253, 1361, 1656, 3620
BstSLI GKGCMC 2 cut(s) 15, 3664
BstUI CGCG 4 cut(s) 920, 4278, 4310, 4329
BstV1I GCAGC 4 cut(s) 1079, 1422, 2998, 3190
BstV2I GAAGAC 4 cut(s) 736, 2108, 2960, 3302
BstX2I RGATCY 7 cut(s) 290, 521, 551, 1696, 2812, 3508, 3543
BstXI CCANNNNNNTGG 1 cut(s) 244
BstYI RGATCY 7 cut(s) 290, 521, 551, 1696, 2812, 3508, 3543
BsuI GTATCC 1 cut(s) 308
BsuRI GGCC 6 cut(s) 25, 74, 2453, 3186, 3779, 4181
BtgZI GCGATG 1 cut(s) 2821
BtsCI GGATG 6 cut(s) 98, 1180, 1847, 3062, 3448, 4039
BtsI GCAGTG 1 cut(s) 168
BtuMI TCGCGA 1 cut(s) 4278
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 4 cut(s) 27, 1041, 3201, 4280
CaiI CAGNNNCTG 2 cut(s) 1309, 3335
CciI TCATGA 2 cut(s) 496, 4054
CfoI GCGC 3 cut(s) 171, 4284, 4329
Cfr13I GGNCC 6 cut(s) 73, 82, 2128, 2353, 3778, 4180
CseI GACGC 1 cut(s) 926
CsiI ACCWGGT 1 cut(s) 1834
Csp6I GTAC 5 cut(s) 539, 759, 1991, 2306, 4168
CviQI GTAC 5 cut(s) 539, 759, 1991, 2306, 4168
DraI TTTAAA 3 cut(s) 871, 885, 1425
DraIII CACNNNGTG 2 cut(s) 1838, 3355
DriI GACNNNNNGTC 1 cut(s) 511
Eam1105I GACNNNNNGTC 1 cut(s) 511
Ecl136II GAGCTC 2 cut(s) 1783, 3688
Eco130I CCWWGG 3 cut(s) 237, 301, 4359
Eco147I AGGCCT 2 cut(s) 25, 3186
Eco24I GRGCYC 3 cut(s) 665, 1785, 3690
Eco32I GATATC 2 cut(s) 787, 2221
Eco47I GGWCC 3 cut(s) 82, 2128, 2353
Eco47III AGCGCT 1 cut(s) 4283
Eco53kI GAGCTC 2 cut(s) 1783, 3688
Eco88I CYCGRG 3 cut(s) 688, 2595, 4240
EcoICRI GAGCTC 2 cut(s) 1783, 3688
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoO109I RGGNCCY 3 cut(s) 73, 82, 3778
EcoRI GAATTC 5 cut(s) 853, 1016, 1129, 2281, 2473
EcoRV GATATC 2 cut(s) 787, 2221
EcoT14I CCWWGG 3 cut(s) 237, 301, 4359
EcoT38I GRGCYC 3 cut(s) 665, 1785, 3690
ErhI CCWWGG 3 cut(s) 237, 301, 4359
Esp3I CGTCTC 1 cut(s) 124
FalI AAGNNNNNCTT 8 cut(s) 1044, 1076, 2305, 2337, 4101, 4133, 4143, 4175
FaqI GGGAC 5 cut(s) 68, 110, 403, 670, 1839
FauNDI CATATG 1 cut(s) 1072
FbaI TGATCA 3 cut(s) 1024, 1392, 1701
Fnu4HI GCNGC 7 cut(s) 921, 1068, 1436, 2249, 3012, 3204, 3868
FokI GGATG 6 cut(s) 105, 1187, 1834, 3049, 3455, 4046
FriOI GRGCYC 3 cut(s) 665, 1785, 3690
Fsp4HI GCNGC 7 cut(s) 921, 1068, 1436, 2249, 3012, 3204, 3868
FspBI CTAG 6 cut(s) 1742, 2015, 2183, 2330, 3038, 4253
FspI TGCGCA 1 cut(s) 170
GlaI GCGC 3 cut(s) 170, 4283, 4328
GluI GCNGC 7 cut(s) 921, 1068, 1436, 2249, 3012, 3204, 3868
GsaI CCCAGC 1 cut(s) 2039
GsuI CTGGAG 5 cut(s) 62, 339, 612, 1286, 2735
HaeII RGCGCY 1 cut(s) 4285
HaeIII GGCC 6 cut(s) 25, 74, 2453, 3186, 3779, 4181
HapII CCGG 2 cut(s) 294, 2630
HgaI GACGC 1 cut(s) 926
HhaI GCGC 3 cut(s) 171, 4284, 4329
Hin6I GCGC 3 cut(s) 169, 4282, 4327
HinP1I GCGC 3 cut(s) 169, 4282, 4327
HincII GTYRAC 2 cut(s) 1368, 3907
HindII GTYRAC 2 cut(s) 1368, 3907
HindIII AAGCTT 6 cut(s) 263, 1427, 1793, 1934, 2909, 3323
HpaII CCGG 2 cut(s) 294, 2630
HphI GGTGA 7 cut(s) 736, 1388, 1700, 1850, 3341, 3644, 3902
Hpy166II GTNNAC 8 cut(s) 13, 635, 1368, 1583, 1841, 2348, 3662, 3907
Hpy8I GTNNAC 8 cut(s) 13, 635, 1368, 1583, 1841, 2348, 3662, 3907
Hpy99I CGWCG 2 cut(s) 3939, 4209
HpyCH4IV ACGT 3 cut(s) 3346, 3589, 4074
HpySE526I ACGT 3 cut(s) 3346, 3589, 4074
HspAI GCGC 3 cut(s) 169, 4282, 4327
Ksp22I TGATCA 3 cut(s) 1024, 1392, 1701
LguI GCTCTTC 1 cut(s) 3108
LmnI GCTCC 9 cut(s) 358, 668, 1057, 1306, 1738, 2326, 2632, 2789, 3860
Lsp1109I GCAGC 4 cut(s) 1079, 1422, 2998, 3190
MabI ACCWGGT 1 cut(s) 1834
MaeI CTAG 6 cut(s) 1742, 2015, 2183, 2330, 3038, 4253
MaeII ACGT 3 cut(s) 3346, 3589, 4074
MaeIII GTNAC 8 cut(s) 928, 1180, 1894, 2026, 2357, 3190, 3347, 3477
MfeI CAATTG 4 cut(s) 141, 1115, 1320, 1442
MflI RGATCY 7 cut(s) 290, 521, 551, 1696, 2812, 3508, 3543
MhlI GDGCHC 5 cut(s) 15, 665, 1785, 3664, 3690
MmeI TCCRAC 6 cut(s) 306, 1239, 1323, 3243, 3339, 3453
MroXI GAANNNNTTC 3 cut(s) 844, 2060, 3615
MslI CAYNNNNRTG 2 cut(s) 645, 1676
MspA1I CMGCKG 3 cut(s) 656, 1435, 3724
MspCI CTTAAG 2 cut(s) 68, 1796
MspI CCGG 2 cut(s) 294, 2630
MunI CAATTG 4 cut(s) 141, 1115, 1320, 1442
Mva1269I GAATGC 3 cut(s) 1651, 3484, 3927
MvnI CGCG 4 cut(s) 920, 4278, 4310, 4329
NciI CCSGG 1 cut(s) 2630
NdeI CATATG 1 cut(s) 1072
NlaIV GGNNCC 4 cut(s) 84, 664, 2129, 3871
NmeAIII GCCGAG 1 cut(s) 3697
NmuCI GTSAC 4 cut(s) 2026, 2357, 3190, 3347
NruI TCGCGA 1 cut(s) 4278
NsbI TGCGCA 1 cut(s) 170
NspI RCATGY 2 cut(s) 950, 3989
NspV TTCGAA 1 cut(s) 2064
PaeR7I CTCGAG 2 cut(s) 2595, 4240
PagI TCATGA 2 cut(s) 496, 4054
PceI AGGCCT 2 cut(s) 25, 3186
PciSI GCTCTTC 1 cut(s) 3108
PcsI WCGNNNNNNNCGW 1 cut(s) 1548
PctI GAATGC 3 cut(s) 1651, 3484, 3927
PdmI GAANNNNTTC 3 cut(s) 844, 2060, 3615
PflFI GACNNNGTC 1 cut(s) 4188
PflMI CCANNNNNTGG 4 cut(s) 93, 1309, 1336, 3358
PfoI TCCNGGA 4 cut(s) 589, 821, 1302, 3054
PkrI GCNGC 7 cut(s) 922, 1069, 1437, 2250, 3013, 3205, 3869
PpuMI RGGWCCY 1 cut(s) 82
PshAI GACNNNNGTC 1 cut(s) 3302
PshBI ATTAAT 3 cut(s) 828, 2258, 3372
Psp124BI GAGCTC 2 cut(s) 1785, 3690
Psp5II RGGWCCY 1 cut(s) 82
PspFI CCCAGC 1 cut(s) 2035
PspN4I GGNNCC 4 cut(s) 84, 664, 2129, 3871
PspPI GGNCC 6 cut(s) 73, 82, 2128, 2353, 3778, 4180
PspPPI RGGWCCY 1 cut(s) 82
PspXI VCTCGAGB 1 cut(s) 4240
PstI CTGCAG 3 cut(s) 402, 1257, 3624
PstNI CAGNNNCTG 2 cut(s) 1309, 3335
PsuI RGATCY 7 cut(s) 290, 521, 551, 1696, 2812, 3508, 3543
PsyI GACNNNGTC 1 cut(s) 4188
PvuII CAGCTG 2 cut(s) 1435, 3724
RruI TCGCGA 1 cut(s) 4278
RsaI GTAC 5 cut(s) 540, 760, 1992, 2307, 4169
RsaNI GTAC 5 cut(s) 539, 759, 1991, 2306, 4168
RseI CAYNNNNRTG 2 cut(s) 645, 1676
SacI GAGCTC 2 cut(s) 1785, 3690
SapI GCTCTTC 1 cut(s) 3108
SatI GCNGC 7 cut(s) 921, 1068, 1436, 2249, 3012, 3204, 3868
Sau96I GGNCC 6 cut(s) 73, 82, 2128, 2353, 3778, 4180
SduI GDGCHC 5 cut(s) 15, 665, 1785, 3664, 3690
SexAI ACCWGGT 1 cut(s) 1834
SfcI CTRYAG 6 cut(s) 123, 398, 1253, 1361, 1656, 3620
Sfr274I CTCGAG 2 cut(s) 2595, 4240
SfuI TTCGAA 1 cut(s) 2064
SinI GGWCC 3 cut(s) 82, 2128, 2353
SlaI CTCGAG 2 cut(s) 2595, 4240
SmiMI CAYNNNNRTG 2 cut(s) 645, 1676
SmlI CTYRAG 9 cut(s) 68, 1124, 1796, 2078, 2595, 3018, 3092, 3740, 4240
SmoI CTYRAG 9 cut(s) 68, 1124, 1796, 2078, 2595, 3018, 3092, 3740, 4240
SseBI AGGCCT 2 cut(s) 25, 3186
SsiI CCGC 8 cut(s) 656, 920, 950, 1475, 1715, 2248, 3867, 4310
SspI AATATT 2 cut(s) 1282, 3151
SspMI CTAG 6 cut(s) 1742, 2015, 2183, 2330, 3038, 4253
SstI GAGCTC 2 cut(s) 1785, 3690
StuI AGGCCT 2 cut(s) 25, 3186
StyI CCWWGG 3 cut(s) 237, 301, 4359
TaiI ACGT 3 cut(s) 3349, 3592, 4077
TatI WGTACW 3 cut(s) 538, 758, 1990
TauI GCSGC 3 cut(s) 923, 2251, 3870
TseFI GTSAC 4 cut(s) 2026, 2357, 3190, 3347
TseI GCWGC 4 cut(s) 1067, 1435, 3011, 3203
Tsp45I GTSAC 4 cut(s) 2026, 2357, 3190, 3347
TspGWI ACGGA 3 cut(s) 189, 1540, 2725
Tth111I GACNNNGTC 1 cut(s) 4188
Van91I CCANNNNNTGG 4 cut(s) 93, 1309, 1336, 3358
Vha464I CTTAAG 2 cut(s) 68, 1796
VneI GTGCAC 2 cut(s) 11, 3660
VpaK11BI GGWCC 3 cut(s) 82, 2128, 2353
VspI ATTAAT 3 cut(s) 828, 2258, 3372
XagI CCTNNNNNAGG 1 cut(s) 305
XbaI TCTAGA 1 cut(s) 4252
XceI RCATGY 2 cut(s) 950, 3989
XcmI CCANNNNNNNNNTGG 1 cut(s) 3436
XhoI CTCGAG 2 cut(s) 2595, 4240
XmnI GAANNNNTTC 3 cut(s) 844, 2060, 3615
XspI CTAG 6 cut(s) 1742, 2015, 2183, 2330, 3038, 4253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.