RchiOBHm_Chr3g0471201

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0008872
Physical Location & Seq
Reverse (-)
17061906 .. 17068883
6978 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43694

Sequence Viewer

Length: 4314 bp
ATGTTTAGGATACACAGGAACAGGCCGGCGAAGTCAGGCGAGAGAATCGATTTCAAGTTCTCTCAGTTCAAAGCCGTTCAGGTGCCGAGAGGATGGGACAAGCTGTATGTGTCCATTGTGTCTGTGGAAACTGGGAAGACAATTGCTAAGTCGAGCAAAGCGGCGGTGCGGAATGGAAGCTGTCAATGGAGTGAGGCGCTTTCCGAGTCTATATGGATTTCGCAGGATGATTCTTCCAAGGAGATGGAAGATTCTTTCTTCAAGCTGGTGGTTGCCATGGGATCGGCGAGATCTGGAATCCTTGGAGAGGCTACAGTCAATATGTCGGATTATATAACTTCAAGTTCTACTGCTCCAGTTTCGGTTCCATTGAAAAAGTGCAACTATGGGACAGTTTTACAGGTGAAAATTAACTGCCTAAAGCCAAGAGCAAGGGTCAGGGATGAAGAGTCAAAAGAGACAAGCAGTCATTTGGAAGAACAAAATGGAACTGGTAATGATGTGGACGGCAAATCAGATGGATCCGGTAGTACATTTGGAAGGAGTCTCGGATCTTCCTCCACAAAAGATTTTGGCTTGACCTCAAATCCTGGAGAACCTGGAAGTAGGGGAAGTAGTTTCTCTGCTGCAGGTTCACTTTGCAGCTATGAGTCAGCAGAGGGTTCCATAAGAAGGGATCCTATTCTTCTAGGAAGCAACCTTACTGGTGAAGGGAACCTCCTGATTGGAAGACAAGATTCAACCAGCTCTCAAACTAGTTCAATGCCTGGCAATTTTCCAGCCATTTCTTCTCCATCCAATCATTCATCATTTAACTCCAGGATCACAGGTTCAGGAAATCATTCTCAGAATTCTCGCAGAGATATTCCAGAATCACCTTTAAGAACCACTAGTTCTTCTAAAAACCTTCTGGAAACCGCAGAAGTTACTATTGAGGAGCTACATGCAGAAGCTAAGATGTGGGAGAGGAATGCACGGAAACTAATGCTTGATCTGGATATACTGAGGACAGAATTTTCTGATCAGTCAAAAAAGCAGGCAAATTTGAATATGGAGCTTTCAGCAGCATATGCTGAACGTGATAGCTTGAAGAAAGAAGTTGAACACTTAAAAGTGTCATTTGAGAGTTCAGTGATGAGACAAACAGCCAGCGAGGATTTATCTCAAGGTGGAGTGTCTCATATAGAGAAGGCATTACAAGATGAACTGAAGTTTCAAAAAGAATCCATTGCCAATCTGGACCTGCAGTTGAAAAGGAGCCAAGAATCAAATATTGAGCTGGTTTCCGTTCTCCAGGAGCTGGAAGAGACAATGGAAGAACAAAAGATGGAGTTAGAGAATCTATCAGAACTGCAATCAAAATTTATTGAAATGGAAAATTCAATCCAAATAACTACAGAGGAGAACAGCAATCTAACGCGTCAGCTGCAAAAATTGCAGGAATCAGAGAACAAGTTGCAAGATATGGTGCAGCAGCTGGAACAGGCTCTGGAAGAGAAAAACCGTGATGTAGAGAAGGGGTCAGGTCTGGAAAAACGAAGCCTTTCGGATATTGAGACAGAATACAGCAGTACAATATTCGATAAAGAAGAAGAGATTATTCAATTGAGAGAAAAGCTGTCTGAGTCTCTCAAAGAAAGCCATTCTGCAGAATTGAGGTCCATTACCATGAATGGAGGTGAAACAGATCTGATCAGACAAATTGAAGTCTTGAAAGAAAAGTTGCAGGAGCTAGAGACAGATTGTAATGAGCTGACGGATGAAAACCTTGAACTCTTATTCAAGCTTAAGGAAGCAAAGAACAATTCTGCAGGGGGACATGCACCTGTTGATCTTCCCACCACTGAGCTTTTGGTGGATTCTTTTACCAGCTTTGAGTCCAAAGTGACTGAACGGAAATCCCACATACAAAATGCAGAGGAGAATTGCAATAAGATGGTGCTTGGGGAGATTACGAATAATCATGATCTTTCAATTCAAGTACTTGAGAGTTTAAAAATGGAACTCGAAATTAAAGTTACAGATCTGGGGAAAGAATTGACAGAGAAAAGGTCTGAGATAGCCAAACTGGAGGATAACTGGCTAACCAAAGAAGAAGAAACCAGGGCTCTACGACAGGTGCACAATGAATTAGAGGCTCAGTTTTCCAATCTACAAAGGGAAAAAGTCGAGCTAGAAGAACACATGGAAATCGTGCTAAGAGAAAGTGAGCTCACCACCAAGTGTTTGAATGATTTGCGAAATGATCTAGTGGTGCTCAGCAGCAATGTGGATTCGCATGTTTCTACCAATAAGATACTTGAAAAGAAATCTTCAGAGCTTGAAGCTGATAAATATGAACTGGAACTTCACTTGTCTGAGCTTGAACAACAAAACACACAATTATCAGAACGTATTTCTGCTCTAGAAGCTCAGCTAAGATGTTTGACAGATGAAAATGAGGCTAATCAATTAGAACTAGAGAATTCCAAATCGTATTCTCTAAAGCTCCAAGATGAAATCATCAGCTTAAAAGTTGAGATGGAATCTGATAAAGTTGAATTGAAACAGAAATTACAGGAATTGGAAAGTCAGTGGTCAGAAGCTCGAGAAGAATGTGAATTTCTTAGAAGGGCAAATCCAAAGTTACAAGCTACCGTGGAGACCCTCATTGAAGAGTGCAATTCACTTGAGAAATCAAATGAGGAGCTGAGAACACAAAAGCTGGCGTTGCATGAGCAATGTACCTACTTGGAGGCAAGGTTGACTGAATCTCACAAGCGCTTTACAGATTGCTCGAAAAGAGTTGAAGTGTTGGAACAAGATCTATCTTTGATGATGGAAAGCATTGCCTCAAAAGAGAAAATCCTAAATTCGGAATTAGATGCACTTCGTGATGAAACCATCCAACATAAGGAGAAACTTATTTCGGAAGAGAGCTTGTTGAATAAAATGTATTTGGAGAAGGCAGTTGAAGTTGAGAACCTTCAGCAAGAGGTAGAACAACTCACCAAGCAACTTTCTGAGACTCATAAAGAAAGCGAAGAACTAGCTTCAGGTGCTTTCCACGAAGCATCCAGATTACGAGCTGAGAAGGTAGATCTTCAGTCTGCTCTGCTAGAGGTTCAATCTAGAGCGATACAGACTGAGAATGAACTCAATATTATGAGAGCAGAATACGAAGAGAAGCTACAAGGCCTGTCAGTTGATCTTACTGCTTCTAAACAAATCCAGGAAACAATGATGGCTGACCATGAAAGGCTATTGAAGTTGTTGGAGAATTACAAGTCAAGTGAAGCAAAATTGAAGACCACTGTGAACAATCTGGAACTAAAACTTACAGTTTCTGATTATGAACAGCAACAGCTGGTGGAAGAATCTACCAATCTGAAGGTTCAACTGCAGAATTTAACTCATTGTCAGGATGAAATGTTGGCTGATGAAGTGTTGGCTATAAAGAATGAGCTTGACGCGATCAAGCTTGAGAAAGAGAAATTGGAAGCACTGCTGGATTCCGTGTCTGAGGAATATGAATATCTGAAGGCAGAGAAGAATTCATTCCTCGAGAAGATTTCAACATTGGATATGGTCTTATCTGAATTGGAGGATTGCAAACATAATAAAGCCGTCTTGGAGAGGAAAGTTCTGCGGATGGAAGGTGATCTAATTGCGAAAGAGGCTTTATTTGCACAAGATGCTGAGCTGAAGAATGAGCTCAACCAGTTCAGAAGAGCCAACGAGCAGTATCAACAAAAACTACAGCTACTCGAGGAGGAGAAAGATGAGTGCCTAAGAAGATCTCAGTCCCTTGAACAAGAATTGAAACTAATTAGGGAAGAAAAACAGAACCAGAGGGATCTCAAGTCAAGAAGCAGTGCCAAAGTCACTGAAGATAAGAAGCTCTCAAAAAATGAAATGGGGAAGAATAGCAGTCATCGTCGTGATAATAGAAGGAAGCCACTAGTGAAGAATGGTCAGGATGATAATGGAATTGACAGCCATGATGCAAGTCCTCGTGATGTTGGAGTTGACTCTGGTTTGAAGATTAAGATGCTTGAGGATGAACTTGTTAAGGCATTGGAAGCAAATAACACTTATAAACTTCAACTTGATAGGCTAACCCGACACAACCATGCGGATGCTCATCAAAATTCAAGAGCCGAAGTAGTGGCAAAAGATAGATTTGAACGCTCGAAATCATCTCTAGAGACAGAATTAAAAGAGATTCGTGAGCGCTACTTGAACATGAGCCTGAGATATGCAGAGGTAGAAGCTGAGCGTGAGGAACTAGTGATGAAGCTCAAGCAATCCAAGAGTGGAAAAAGGTGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1437

Amino Acids

163.84

Weight (kDa)

5.0

Isoelectric Point (pI)

55.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 28 - 140 4e-12 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4077
Acc36I ACCTGC 2 cut(s) 620, 1251
AccB1I GGYRCC 1 cut(s) 82
AccB7I CCANNNNNTGG 1 cut(s) 1300
AccII CGCG 2 cut(s) 1420, 3452
AciI CCGC 6 cut(s) 161, 164, 169, 918, 3628, 4115
AclWI GGATC 8 cut(s) 289, 516, 529, 559, 671, 684, 830, 3843
AcuI CTGAAG 9 cut(s) 1229, 2298, 2951, 3018, 3068, 3389, 3539, 3704, 3888
AdeI CACNNNGTG 2 cut(s) 2223, 2873
AfaI GTAC 4 cut(s) 532, 1573, 1983, 2725
AfeI AGCGCT 2 cut(s) 2762, 4214
AfiI CCNNNNNNNGG 5 cut(s) 307, 672, 1300, 2854, 2893
AflII CTTAAG 1 cut(s) 1787
AflIII ACRYGT 1 cut(s) 1418
AhdI GACNNNNNGTC 1 cut(s) 465
AhlI ACTAGT 4 cut(s) 755, 890, 3940, 4267
AjnI CCWGG 7 cut(s) 589, 598, 766, 818, 1293, 2102, 3210
AjuI GAANNNNNNNTTGG 2 cut(s) 3458, 3490
AloI GAACNNNNNNTCC 2 cut(s) 814, 846
Alw21I GWGCWC 4 cut(s) 2124, 2214, 2259, 3696
Alw44I GTGCAC 1 cut(s) 2120
AlwI GGATC 8 cut(s) 289, 516, 529, 559, 671, 684, 830, 3843
AlwNI CAGNNNCTG 4 cut(s) 1300, 1477, 1489, 3326
Ama87I CYCGRG 3 cut(s) 2586, 3542, 3746
Aor51HI AGCGCT 2 cut(s) 2762, 4214
AoxI GGCC 2 cut(s) 23, 3175
ApaLI GTGCAC 1 cut(s) 2120
ApeKI GCWGC 7 cut(s) 626, 642, 1064, 1426, 1471, 1474, 2262
Asp700I GAANNNNTTC 3 cut(s) 841, 1543, 3536
AspLEI GCGC 3 cut(s) 199, 2763, 4215
AspS9I GGNCC 2 cut(s) 1240, 1659
AsuHPI GGTGA 7 cut(s) 415, 719, 867, 1691, 2206, 2980, 3650
AvaI CYCGRG 3 cut(s) 2586, 3542, 3746
AvaII GGWCC 2 cut(s) 1240, 1659
BaeGI GKGCMC 1 cut(s) 2124
BamHI GGATCC 2 cut(s) 521, 676
BanI GGYRCC 1 cut(s) 82
BanII GRGCYC 3 cut(s) 2110, 2214, 3696
BarI GAAGNNNNNNTAC 4 cut(s) 685, 717, 3153, 3185
BauI CACGAG 1 cut(s) 3993
BbsI GAAGAC 3 cut(s) 143, 736, 3293
Bbv12I GWGCWC 4 cut(s) 2124, 2214, 2259, 3696
BbvI GCAGC 7 cut(s) 613, 654, 1076, 1413, 1483, 1486, 2274
BceAI ACGGC 3 cut(s) 59, 523, 3590
BciT130I CCWGG 7 cut(s) 591, 600, 768, 820, 1295, 2104, 3212
BciVI GTATCC 1 cut(s) 3
BclI TGATCA 2 cut(s) 1021, 1692
BcuI ACTAGT 4 cut(s) 755, 890, 3940, 4267
BfmI CTRYAG 8 cut(s) 312, 627, 1244, 1395, 1647, 1809, 3380, 3737
BfoI RGCGCY 3 cut(s) 200, 2764, 4216
BfrI CTTAAG 1 cut(s) 1787
BfuAI ACCTGC 2 cut(s) 620, 1251
BfuI GTATCC 1 cut(s) 3
BglII AGATCT 6 cut(s) 290, 1687, 2023, 2803, 3079, 3776
BisI GCNGC 8 cut(s) 162, 627, 643, 1065, 1427, 1472, 1475, 2263
BlpI GCTNAGC 4 cut(s) 2258, 2412, 3678, 4254
BlsI GCNGC 8 cut(s) 163, 628, 644, 1066, 1428, 1473, 1476, 2264
BmcAI AGTACT 1 cut(s) 1983
Bme1390I CCNGG 7 cut(s) 591, 600, 768, 820, 1295, 2104, 3212
Bme18I GGWCC 2 cut(s) 1240, 1659
BmeRI GACNNNNNGTC 1 cut(s) 465
BmeT110I CYCGRG 3 cut(s) 2586, 3542, 3746
BmgT120I GGNCC 2 cut(s) 1240, 1659
BmiI GGNNCC 7 cut(s) 84, 366, 523, 664, 678, 716, 1259
BmrFI CCNGG 7 cut(s) 591, 600, 768, 820, 1295, 2104, 3212
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 6 cut(s) 2854, 3062, 3664, 3973, 4020, 4108
BmuI ACTGGG 1 cut(s) 141
BpiI GAAGAC 3 cut(s) 143, 736, 3293
BpmI CTGGAG 5 cut(s) 339, 612, 802, 1277, 2090
Bpu1102I GCTNAGC 4 cut(s) 2258, 2412, 3678, 4254
BpuEI CTTGAG 7 cut(s) 1149, 2006, 2690, 3482, 3824, 4055, 4265
Bsa29I ATCGAT 1 cut(s) 48
BsaBI GATNNNNATC 1 cut(s) 4122
BsaI GGTCTC 1 cut(s) 2636
BsaJI CCNNGG 5 cut(s) 237, 276, 301, 2103, 2637
BsaWI WCCGGW 1 cut(s) 524
BsaXI ACNNNNNCTCC 2 cut(s) 2726, 2756
Bsc4I CCNNNNNNNGG 5 cut(s) 307, 672, 1300, 2854, 2893
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 8 cut(s) 136, 356, 496, 709, 2073, 2084, 2346, 3700
Bse3DI GCAATG 4 cut(s) 1227, 2272, 2726, 2826
Bse8I GATNNNNATC 1 cut(s) 4122
BseBI CCWGG 7 cut(s) 591, 600, 768, 820, 1295, 2104, 3212
BseCI ATCGAT 1 cut(s) 48
BseDI CCNNGG 5 cut(s) 237, 276, 301, 2103, 2637
BseJI GATNNNNATC 1 cut(s) 4122
BseLI CCNNNNNNNGG 5 cut(s) 307, 672, 1300, 2854, 2893
BseMI GCAATG 4 cut(s) 1227, 2272, 2726, 2826
BseNI ACTGG 8 cut(s) 136, 356, 496, 709, 2073, 2084, 2346, 3700
BseRI GAGGAG 6 cut(s) 950, 1415, 1934, 2699, 3764, 3767
BseSI GKGCMC 1 cut(s) 2124
BseXI GCAGC 7 cut(s) 613, 654, 1076, 1413, 1483, 1486, 2274
BsgI GTGCAG 1 cut(s) 1490
Bsh1236I CGCG 2 cut(s) 1420, 3452
BshFI GGCC 2 cut(s) 25, 3177
BshNI GGYRCC 1 cut(s) 82
BshVI ATCGAT 1 cut(s) 48
BsiHKAI GWGCWC 4 cut(s) 2124, 2214, 2259, 3696
BsiHKCI CYCGRG 3 cut(s) 2586, 3542, 3746
BsiSI CCGG 2 cut(s) 26, 525
BslFI GGGAC 4 cut(s) 110, 403, 1830, 3769
BslI CCNNNNNNNGG 5 cut(s) 307, 672, 1300, 2854, 2893
BsmFI GGGAC 4 cut(s) 110, 403, 1830, 3769
BsmI GAATGC 1 cut(s) 976
BsnI GGCC 2 cut(s) 25, 3177
Bso31I GGTCTC 1 cut(s) 2636
BsoBI CYCGRG 3 cut(s) 2586, 3542, 3746
Bsp1286I GDGCHC 5 cut(s) 2110, 2124, 2214, 2259, 3696
Bsp1720I GCTNAGC 4 cut(s) 2258, 2412, 3678, 4254
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 6 cut(s) 161, 164, 169, 918, 3628, 4115
BspANI GGCC 2 cut(s) 25, 3177
BspDI ATCGAT 1 cut(s) 48
BspFNI CGCG 2 cut(s) 1420, 3452
BspHI TCATGA 1 cut(s) 1963
BspLI GGNNCC 7 cut(s) 84, 366, 523, 664, 678, 716, 1259
BspMAI CTGCAG 5 cut(s) 631, 1248, 1651, 1813, 3384
BspMI ACCTGC 2 cut(s) 620, 1251
BspPI GGATC 8 cut(s) 289, 516, 529, 559, 671, 684, 830, 3843
BspQI GCTCTTC 1 cut(s) 3703
BspT107I GGYRCC 1 cut(s) 82
BspTI CTTAAG 1 cut(s) 1787
BspTNI GGTCTC 1 cut(s) 2636
BsrDI GCAATG 4 cut(s) 1227, 2272, 2726, 2826
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 8 cut(s) 136, 356, 496, 709, 2073, 2084, 2346, 3700
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 5 cut(s) 237, 276, 301, 2103, 2637
BssSI CACGAG 1 cut(s) 3993
BssT1I CCWWGG 3 cut(s) 237, 276, 301
Bst2BI CACGAG 1 cut(s) 3993
Bst2UI CCWGG 7 cut(s) 591, 600, 768, 820, 1295, 2104, 3212
Bst4CI ACNGT 6 cut(s) 316, 394, 1505, 2638, 3295, 3322
Bst6I CTCTTC 8 cut(s) 441, 1299, 1488, 1587, 2649, 2907, 3156, 3703
BstAFI CTTAAG 1 cut(s) 1787
BstAPI GCANNNNNTGC 3 cut(s) 1070, 1435, 3674
BstC8I GCNNGC 4 cut(s) 27, 1038, 1150, 2706
BstDSI CCRYGG 2 cut(s) 276, 2637
BstENI CCTNNNNNAGG 1 cut(s) 305
BstFNI CGCG 2 cut(s) 1420, 3452
BstH2I RGCGCY 3 cut(s) 200, 2764, 4216
BstHHI GCGC 3 cut(s) 199, 2763, 4215
BstNI CCWGG 7 cut(s) 591, 600, 768, 820, 1295, 2104, 3212
BstNSI RCATGY 3 cut(s) 947, 1823, 2282
BstSCI CCNGG 7 cut(s) 589, 598, 766, 818, 1293, 2102, 3210
BstSFI CTRYAG 8 cut(s) 312, 627, 1244, 1395, 1647, 1809, 3380, 3737
BstSLI GKGCMC 1 cut(s) 2124
BstUI CGCG 2 cut(s) 1420, 3452
BstV1I GCAGC 7 cut(s) 613, 654, 1076, 1413, 1483, 1486, 2274
BstV2I GAAGAC 3 cut(s) 143, 736, 3293
BstXI CCANNNNNNTGG 1 cut(s) 244
Bsu15I ATCGAT 1 cut(s) 48
BsuI GTATCC 1 cut(s) 3
BsuRI GGCC 2 cut(s) 25, 3177
BsuTUI ATCGAT 1 cut(s) 48
BtgI CCRYGG 2 cut(s) 276, 2637
BtsI GCAGTG 2 cut(s) 3482, 3859
BtsIMutI CAGTG 7 cut(s) 1137, 1842, 2579, 3291, 3482, 3859, 3864
BveI ACCTGC 2 cut(s) 620, 1251
Cac8I GCNNGC 4 cut(s) 27, 1038, 1150, 2706
CaiI CAGNNNCTG 4 cut(s) 1300, 1477, 1489, 3326
CciI TCATGA 1 cut(s) 1963
CfoI GCGC 3 cut(s) 199, 2763, 4215
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 2 cut(s) 1240, 1659
ClaI ATCGAT 1 cut(s) 48
CseI GACGC 2 cut(s) 1409, 3458
Csp6I GTAC 4 cut(s) 531, 1572, 1982, 2724
CviQI GTAC 4 cut(s) 531, 1572, 1982, 2724
DraI TTTAAA 1 cut(s) 1995
DraIII CACNNNGTG 2 cut(s) 2223, 2873
DriI GACNNNNNGTC 1 cut(s) 465
Eam1104I CTCTTC 8 cut(s) 441, 1299, 1488, 1587, 2649, 2907, 3156, 3703
Eam1105I GACNNNNNGTC 1 cut(s) 465
EarI CTCTTC 8 cut(s) 441, 1299, 1488, 1587, 2649, 2907, 3156, 3703
Ecl136II GAGCTC 2 cut(s) 2212, 3694
Eco130I CCWWGG 3 cut(s) 237, 276, 301
Eco147I AGGCCT 1 cut(s) 3177
Eco24I GRGCYC 3 cut(s) 2110, 2214, 3696
Eco31I GGTCTC 1 cut(s) 2636
Eco47I GGWCC 2 cut(s) 1240, 1659
Eco47III AGCGCT 2 cut(s) 2762, 4214
Eco53kI GAGCTC 2 cut(s) 2212, 3694
Eco57I CTGAAG 9 cut(s) 1229, 2298, 2951, 3018, 3068, 3389, 3539, 3704, 3888
Eco88I CYCGRG 3 cut(s) 2586, 3542, 3746
EcoICRI GAGCTC 2 cut(s) 2212, 3694
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoRI GAATTC 3 cut(s) 850, 2464, 3532
EcoRII CCWGG 7 cut(s) 589, 598, 766, 818, 1293, 2102, 3210
EcoT14I CCWWGG 3 cut(s) 237, 276, 301
EcoT38I GRGCYC 3 cut(s) 2110, 2214, 3696
ErhI CCWWGG 3 cut(s) 237, 276, 301
FaqI GGGAC 4 cut(s) 110, 403, 1830, 3769
FauNDI CATATG 1 cut(s) 1069
FbaI TGATCA 2 cut(s) 1021, 1692
Fnu4HI GCNGC 8 cut(s) 162, 627, 643, 1065, 1427, 1472, 1475, 2263
FriOI GRGCYC 3 cut(s) 2110, 2214, 3696
Fsp4HI GCNGC 8 cut(s) 162, 627, 643, 1065, 1427, 1472, 1475, 2263
GlaI GCGC 3 cut(s) 198, 2762, 4214
GluI GCNGC 8 cut(s) 162, 627, 643, 1065, 1427, 1472, 1475, 2263
GsuI CTGGAG 5 cut(s) 339, 612, 802, 1277, 2090
HaeII RGCGCY 3 cut(s) 200, 2764, 4216
HaeIII GGCC 2 cut(s) 25, 3177
HapII CCGG 2 cut(s) 26, 525
HgaI GACGC 2 cut(s) 1409, 3458
HhaI GCGC 3 cut(s) 199, 2763, 4215
Hin6I GCGC 3 cut(s) 197, 2761, 4213
HinP1I GCGC 3 cut(s) 197, 2761, 4213
HincII GTYRAC 2 cut(s) 2745, 4009
HindII GTYRAC 2 cut(s) 2745, 4009
HindIII AAGCTT 2 cut(s) 1784, 3458
HpaII CCGG 2 cut(s) 26, 525
HphI GGTGA 7 cut(s) 415, 719, 867, 1691, 2206, 2980, 3650
Hpy166II GTNNAC 6 cut(s) 505, 635, 2122, 2745, 3298, 4009
Hpy8I GTNNAC 6 cut(s) 505, 635, 2122, 2745, 3298, 4009
Hpy99I CGWCG 1 cut(s) 3921
HpyCH4III ACNGT 6 cut(s) 316, 394, 1505, 2638, 3295, 3322
HpyCH4IV ACGT 2 cut(s) 1078, 2392
HpySE526I ACGT 2 cut(s) 1078, 2392
HspAI GCGC 3 cut(s) 197, 2761, 4213
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Ksp22I TGATCA 2 cut(s) 1021, 1692
LguI GCTCTTC 1 cut(s) 3703
LmnI GCTCC 8 cut(s) 358, 937, 1054, 1257, 1297, 1729, 2493, 2686
Lsp1109I GCAGC 7 cut(s) 613, 654, 1076, 1413, 1483, 1486, 2274
LweI GCATC 6 cut(s) 2854, 3062, 3664, 3973, 4020, 4108
MaeII ACGT 2 cut(s) 1078, 2392
MaeIII GTNAC 5 cut(s) 925, 1885, 2017, 2625, 3862
MfeI CAATTG 2 cut(s) 141, 1604
MhlI GDGCHC 5 cut(s) 2110, 2124, 2214, 2259, 3696
MluI ACGCGT 1 cut(s) 1418
MlyI GAGTC 8 cut(s) 215, 458, 553, 659, 1634, 1886, 3001, 4004
MmeI TCCRAC 5 cut(s) 306, 2775, 2911, 3234, 3982
MroNI GCCGGC 1 cut(s) 25
MroXI GAANNNNTTC 3 cut(s) 841, 1543, 3536
MslI CAYNNNNRTG 4 cut(s) 1667, 3918, 4110, 4116
MspA1I CMGCKG 3 cut(s) 1426, 1477, 3346
MspCI CTTAAG 1 cut(s) 1787
MspI CCGG 2 cut(s) 26, 525
MspR9I CCNGG 7 cut(s) 591, 600, 768, 820, 1295, 2104, 3212
MunI CAATTG 2 cut(s) 141, 1604
Mva1269I GAATGC 1 cut(s) 976
MvaI CCWGG 7 cut(s) 591, 600, 768, 820, 1295, 2104, 3212
MvnI CGCG 2 cut(s) 1420, 3452
NaeI GCCGGC 1 cut(s) 27
NcoI CCATGG 1 cut(s) 276
NdeI CATATG 1 cut(s) 1069
NgoMIV GCCGGC 1 cut(s) 25
NlaIV GGNNCC 7 cut(s) 84, 366, 523, 664, 678, 716, 1259
NmeAIII GCCGAG 1 cut(s) 111
NmuCI GTSAC 2 cut(s) 1885, 3862
NspI RCATGY 3 cut(s) 947, 1823, 2282
PaeR7I CTCGAG 3 cut(s) 2586, 3542, 3746
PagI TCATGA 1 cut(s) 1963
PceI AGGCCT 1 cut(s) 3177
PciSI GCTCTTC 1 cut(s) 3703
PctI GAATGC 1 cut(s) 976
PdiI GCCGGC 1 cut(s) 27
PdmI GAANNNNTTC 3 cut(s) 841, 1543, 3536
PflMI CCANNNNNTGG 1 cut(s) 1300
PfoI TCCNGGA 4 cut(s) 589, 818, 1293, 3210
PkrI GCNGC 8 cut(s) 163, 628, 644, 1066, 1428, 1473, 1476, 2264
PleI GAGTC 8 cut(s) 214, 457, 552, 658, 1633, 1885, 3001, 4004
PpsI GAGTC 8 cut(s) 214, 457, 552, 658, 1633, 1885, 3001, 4004
PsiI TTATAA 1 cut(s) 4077
Psp124BI GAGCTC 2 cut(s) 2214, 3696
Psp6I CCWGG 7 cut(s) 589, 598, 766, 818, 1293, 2102, 3210
PspGI CCWGG 7 cut(s) 589, 598, 766, 818, 1293, 2102, 3210
PspN4I GGNNCC 7 cut(s) 84, 366, 523, 664, 678, 716, 1259
PspPI GGNCC 2 cut(s) 1240, 1659
PspXI VCTCGAGB 1 cut(s) 3746
PstI CTGCAG 5 cut(s) 631, 1248, 1651, 1813, 3384
PstNI CAGNNNCTG 4 cut(s) 1300, 1477, 1489, 3326
PvuII CAGCTG 3 cut(s) 1426, 1477, 3346
RsaI GTAC 4 cut(s) 532, 1573, 1983, 2725
RsaNI GTAC 4 cut(s) 531, 1572, 1982, 2724
RseI CAYNNNNRTG 4 cut(s) 1667, 3918, 4110, 4116
SacI GAGCTC 2 cut(s) 2214, 3696
SapI GCTCTTC 1 cut(s) 3703
SatI GCNGC 8 cut(s) 162, 627, 643, 1065, 1427, 1472, 1475, 2263
Sau96I GGNCC 2 cut(s) 1240, 1659
ScaI AGTACT 1 cut(s) 1983
SchI GAGTC 8 cut(s) 215, 458, 553, 659, 1634, 1886, 3001, 4004
ScrFI CCNGG 7 cut(s) 591, 600, 768, 820, 1295, 2104, 3212
SduI GDGCHC 5 cut(s) 2110, 2124, 2214, 2259, 3696
SfaNI GCATC 6 cut(s) 2854, 3062, 3664, 3973, 4020, 4108
SfcI CTRYAG 8 cut(s) 312, 627, 1244, 1395, 1647, 1809, 3380, 3737
Sfr274I CTCGAG 3 cut(s) 2586, 3542, 3746
SinI GGWCC 2 cut(s) 1240, 1659
SlaI CTCGAG 3 cut(s) 2586, 3542, 3746
SmiMI CAYNNNNRTG 4 cut(s) 1667, 3918, 4110, 4116
SpeI ACTAGT 4 cut(s) 755, 890, 3940, 4267
SseBI AGGCCT 1 cut(s) 3177
SsiI CCGC 6 cut(s) 161, 164, 169, 918, 3628, 4115
SspI AATATT 3 cut(s) 1273, 1578, 3142
SstI GAGCTC 2 cut(s) 2214, 3696
StuI AGGCCT 1 cut(s) 3177
StyD4I CCNGG 7 cut(s) 589, 598, 766, 818, 1293, 2102, 3210
StyI CCWWGG 3 cut(s) 237, 276, 301
TaaI ACNGT 6 cut(s) 316, 394, 1505, 2638, 3295, 3322
TaiI ACGT 2 cut(s) 1081, 2395
TatI WGTACW 3 cut(s) 530, 1571, 1981
TauI GCSGC 1 cut(s) 164
TscAI CASTG 7 cut(s) 1137, 1849, 2579, 3298, 3489, 3859, 3871
TseFI GTSAC 2 cut(s) 1885, 3862
TseI GCWGC 7 cut(s) 626, 642, 1064, 1426, 1471, 1474, 2262
Tsp45I GTSAC 2 cut(s) 1885, 3862
TspGWI ACGGA 5 cut(s) 991, 1276, 1772, 1909, 3484
TspRI CASTG 7 cut(s) 1137, 1849, 2579, 3298, 3489, 3859, 3871
Van91I CCANNNNNTGG 1 cut(s) 1300
Vha464I CTTAAG 1 cut(s) 1787
VneI GTGCAC 1 cut(s) 2120
VpaK11BI GGWCC 2 cut(s) 1240, 1659
XagI CCTNNNNNAGG 1 cut(s) 305
XbaI TCTAGA 3 cut(s) 2404, 3110, 4183
XceI RCATGY 3 cut(s) 947, 1823, 2282
XcmI CCANNNNNNNNNTGG 3 cut(s) 121, 1234, 1849
XhoI CTCGAG 3 cut(s) 2586, 3542, 3746
XmnI GAANNNNTTC 3 cut(s) 841, 1543, 3536
ZrmI AGTACT 1 cut(s) 1983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.