Rroxscaffold_6G00410230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008872
Physical Location & Seq
Forward (+)
32661203 .. 32668421
7219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00410230.1

Sequence Viewer

Length: 4404 bp
ATGTTTAGGATACACAGGAGCAGGCCGGCGAAGTCAGGCGAGAGAATCGATTTCAAGTTCTCTCAGTTCAAAGCCGTTCAGGTGCCAAAAGGATGGGACAAGCTGTATGTGTCCATTGTGTCTTTGGAAACTGGGAAGGCAATTGCTAAGTCGAGCAAAGCAGCGGTGCGGAATGGCAGCTGTCAATGGAGTGAGGCGCTTTCCGAGTCTATATGGATTTCGCAGGATGATTCTTCCAAGGAGATGGAAGATTCTTTCTTCAAGCTGGTGGTTGCCATGGGATCGGCTAGATCTGGAATCCTTGGAGAGGCTACAGTCAATATGTCAGATTATATAACTTCAAGTTCCACTGCTCCAATTTCGCTTCCATTGAAAAAGTGCAACTATGGGACAGTTCTACAGGTGAAAATTAATTGCCTAAAGCCAAGAGCAAGGGCCAGGGATGAAGAGTCAAAAGAGACAAGCAGTCATTTGGAAGAACAAAATGGAACTAATAATGATGTGGACGGCAAGTCAGATGGATCCGGTAGTACATTTGGAAGGAGTCTCGGATCTTCCTCCACAAAAGATTTTGGCTTGACCTCAAATCCTGGAGAACCTGGAAGTAGGGGAAGTAGTTTCTCTGCTGCAGGTTCACATTGCAGCTATGAGTCAGCAGAGGGTCCCATAAGAAGGGATCCTATTCTTCTAGGAAGCAACCTTACTGGTGAAGGGAACCTCCTGATTGGAAGACAAGATTCAACCAGCTCTCAAACTAGTTCAATGCCTGGCAATTTTCCAGCCATTTCTTCTCCATCCAATCATTCATCATTTAACTCCAGGATCACAGGTTCAGGAAATCATTCTCAGAATTCTCGCAGAGATATTCCAGAATCACCTTTAAGAACCACTAGTTCTTCTAAAAACCTTCTGGAAACTGCAGAAGTTACTATTGAAGAGCTACATGCAGAAGCTAAGATGTGGGAGAGGAATGCACGGAAACTAATGCTTGATCTGGATATACTGAGGGCAGAATTTTCTGATCAGTCAAAAAGGCAGGCAAATTTGAATATGGAGCTTTCAGCAGCATATGCTGAACGTGATAGCTTGAAGAAAGAAGTTGAACACTTAAAAGTGTCATTTGAAAGTTCAGTGATGAGACAAACAGCCAGCGAGGATTTATCTCAAGGTGGAGTGTCTCATATAGAGAAGGCATTGCAAGATGAACTGAAGTTTCAAAAAGATTCCATTGCCAATCTGGACCTGCAGTTGAAAAGGAGCCAAGAATCAAATATTGAGCTGGTTTCCGTTCTCCAGGAGCTGGAAGAGACAATTGAAGAACAAAAGATGGAGTTAGAGAATCTATCAGAACTACAATCAAAATTTATTGAAATGGAAAATTCAATCCAAATAACTACAGAGGAGAACAGCAATCTAACGCGTCAGCTGCAAAAATTGCAGGAATCAGAGAACAAGTTGCAAGATATGGTGCAGCAGCTGCAACAGGCTCTGGAAGAGAAAAACGGTGATGTAGAGAAGGGGTCGGGTCTGGAAAAACGAAGCCTTTCGGATATTGAGACAGAATACAGCAGTACATTATTCGATAAAGAAGAAGAGATTATTCAATTGAGAGAAAAGCTGTCTGAGTCTCTCAAAGAAAGCCATTCTGCAGAAATGAGGTCCATTCTCATGAATGGAGGTGAAACAGATCTGATCAGACAAATTGAAGTCTTGAAAGAAAAGTTGCAGGAGCTAGAGACAGATTGTAATGAGCTGACGGATGAAAACCTTGAACTCTTATTCAAGCTTAAGGAAGCAAAGAACAATTCTGCAGGGGGACATGCACCTGTTGATCTTCCCACCACTGAGCTTTTGGTGGATTCTTTTACCAGCTTTGAGTCCAAAGTGACTGAACGGAAATCCCACGTACAAAATGCAGAGGAGAATTGCAATAAGATGGTGCTTGGGGAGATTACGAATAATCATGATCTTTCAATTCAAGTGCTTGAGAGTCTAAAAATGGAACTCGAAAGTAAAGTCACAGATCTGGGGAAAGAATTGACAGAGAGAAGGTCTGAGATAGCCAAACTGGAGGATAACTTGCTAACCAAAGAAGAAGAAATCGGGGCTCTACAACAGGTGCACAATGAATTAGAGGCTCAGTTTTCCAATCTACAAAGGGAAAAAGTCGAGCTAGAAGAACACATAGAAATCGTGCTAAGAGAAAGTGAGCTCACCACCAAGTGTTTGAATGATTTGCGAAATGATCTAGTGGTGCTCAGCAGCAATGTGGATTCGCATGTTGCTACCAATAAGATACTTGAAAAGAAATCTTCAGAGCTTGAAGCTGATAAATATGAACTGAAACTTCACTTGTCTGAGCTTGAACAACAAAACTCACAATTATCGGAACGTATTTCTGCTCTAGAAGCTCAGCTAAGATGTTTGACAGATGAAAAGGAGGCTAATCAATTAGAACTAGAGAATTCCAAATCTTATTCTCTAAAGCTCCAAGATGAAATCAGCAGCTTAAAAGTTGAGATGGAATCTGATAAAGTTGAACTGAAACAGAAATTACAGGAATCGCAAAGTCAGTGGTCAGAAGCTCGAGAAGAATGTGAATTTCTTAGAAGGGCAAATCCAAAGTTACAAGCTACTGTGGAGACCCTCATTGAAGAGTGCAATTCGCTTGAGAAATCAAATGAGGAGCTGAGAACACAAAAGCTGGAGTTGCATGAGCAATGTACCTACTTGGAGGCAAGGTTGACTGAATCTCACAAGCGCTTTACAGATTGCTCGAAAAGAGTTGAAGTGTTGGAACAAGATCTATCTTTGATGATGGAAAGCATTGCTTCAAAAGAGAAAATCCTAAATTCGGAATTAGATGCACTTCGTGACGAAACCATCCAACATAAGGAGAAACTTATTTCGGAAGAGAGCTTGTTGAATAAGATGTATTTGGAGAAGGCAGTTGAAGTTGAGAACCTTCAGCAAGAGGTAGAACGACTCACCAAGCAACTTTCTGAGACTCATAAAGAAGGCGAAGAACTAGCTTCAGGTGCTTTCCACGAAGCATCCAGATTACGAGCTGAGAAGGTAGATCTTCAGTCTGCTCTGCTAGAGGTTCAATCTAGAGCGATAGAGACTGAGAATGAACTCAATATTATGAGAGCAGAATATGAAGAGAAGCTACAAGGCCTGTCAGTTGATCTTACTGCTTCTAAACAAATCCAGGAAACAATGATGGCTGACCATGAAAGGCTATTGAAGTTGTTGGAGAATTACAAGTCAAGTGAAGCAAAATTGAAGACCACTGTGAACAATCTAGAACTAAAACTTACAGTTTCTGATTATGAACAGCAACAGCTGGTGGAAGAATCTACCAATCTGAAGGTTCAACTGCAGAATTTAACTCATTGTCAGGATGAAATGTTGGCTGTTAAGAATGAGCTTGATGCAGCTAAGCTTGAGAAAGAGAAATTGGAATCGCTACTGGATACTCAACTACAGAATTTAACTCATTGTCAGGACGAAGTGTTGGCTATAAAGAATGAGCTTGACACGATCAAGCTTGAGAAAGAGAAATTGGAAGCACTGCTGGATTCCGTGTCTGAGGAATATGAATATCTGAAGGCAGAGAAGAATTCATTCCTCGAGAAGATTTCAACATTTGATATGGTCTTATCAGAATTGGAGGATTGCAAACATAATAAAGCTGTCTTGGAGAGGAAAGTTCTGAGGATGGAAGGTGATCTAATTGCAAAAGAGGCTTTATTTGCACAAGATTCTGAGCTGAAGAATGAGCTCAACCAGTTCAGAAGAGCCAACGAGCAGTATCAACAAAAACTACAGCTACTCGAGGAGGAGAAAGATGAGTGCCTAAGAAGATCTCAGTCCCTTGAACAAGAATTGAAACTAATTAGGGAAGAAAAACAGAACCAGAGGGATCTAAAGTCAAGAAGCAGTGCCAAAATCACTGAAGATAAGAAGCTCTCAAAAAATGAAATGGTGAAGAATAGCGGTCATCGTCGTGATAATAGAAGGAAGCCACTAGTGAAGAATGGTCAGGATGATAATGGAATTGACAGCCATGATGCAAGTCCCCATGATGTTGGAGTTGACTCTGGTTTGAAGATTAAGATGCTTGAGGATGAACTTGTTAAGGCATTGGAAGCAAATAACACTTATAAACTTCAACTTGATAGGCTAACCCGACACAACCATGCGGATGCTCGTCAAAATTCAAGAGCCGAAGCAGTGGCAAAAGATAGATTGGAACGCTCGAAATCATCTCTAGAGACAGAATTGAAAGAGATTCGTGAGCGCTACTTGAACATGAGCCTGAGATATGCAGAGGTAGAAGCTGAGCGTGAGGAACTAGTGATGAAGCTCAAGCAATCCAAGAGTGGAAAAAGGTGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1467

Amino Acids

167.0

Weight (kDa)

5.01

Isoelectric Point (pI)

56.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 24 - 140 1.9e-12 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4167
Acc36I ACCTGC 2 cut(s) 620, 1251
AccB1I GGYRCC 1 cut(s) 82
AccB7I CCANNNNNTGG 1 cut(s) 1300
AccII CGCG 1 cut(s) 1420
AciI CCGC 4 cut(s) 164, 169, 3999, 4205
AclWI GGATC 8 cut(s) 289, 516, 529, 559, 671, 684, 830, 3933
AcuI CTGAAG 9 cut(s) 1229, 2298, 2951, 3018, 3068, 3389, 3629, 3794, 3978
AdeI CACNNNGTG 2 cut(s) 2223, 2873
AfaI GTAC 4 cut(s) 532, 1573, 1908, 2725
AfeI AGCGCT 2 cut(s) 2762, 4304
AfiI CCNNNNNNNGG 5 cut(s) 307, 672, 1300, 2854, 2893
AflII CTTAAG 1 cut(s) 1787
AflIII ACRYGT 1 cut(s) 1418
AhdI GACNNNNNGTC 2 cut(s) 465, 511
AhlI ACTAGT 4 cut(s) 755, 890, 4030, 4357
AjnI CCWGG 7 cut(s) 437, 589, 598, 766, 818, 1293, 3210
AjuI GAANNNNNNNTTGG 4 cut(s) 3443, 3475, 3548, 3580
AloI GAACNNNNNNTCC 2 cut(s) 814, 846
Alw21I GWGCWC 4 cut(s) 2124, 2214, 2259, 3786
Alw44I GTGCAC 1 cut(s) 2120
AlwI GGATC 8 cut(s) 289, 516, 529, 559, 671, 684, 830, 3933
AlwNI CAGNNNCTG 4 cut(s) 1300, 1477, 1489, 3326
Ama87I CYCGRG 3 cut(s) 2586, 3632, 3836
Aor51HI AGCGCT 2 cut(s) 2762, 4304
AoxI GGCC 3 cut(s) 23, 435, 3175
ApaLI GTGCAC 1 cut(s) 2120
ArsI GACNNNNNNTTYG 2 cut(s) 2001, 2033
AseI ATTAAT 1 cut(s) 411
Asp700I GAANNNNTTC 3 cut(s) 841, 1543, 3626
AspLEI GCGC 3 cut(s) 199, 2763, 4305
AspS9I GGNCC 4 cut(s) 435, 662, 1240, 1659
AsuHPI GGTGA 9 cut(s) 415, 719, 867, 1517, 1691, 2206, 2980, 3740, 4000
AvaI CYCGRG 3 cut(s) 2586, 3632, 3836
AvaII GGWCC 3 cut(s) 662, 1240, 1659
BaeGI GKGCMC 1 cut(s) 2124
BamHI GGATCC 2 cut(s) 521, 676
BanI GGYRCC 1 cut(s) 82
BanII GRGCYC 3 cut(s) 2110, 2214, 3786
BarI GAAGNNNNNNTAC 4 cut(s) 685, 717, 3153, 3185
BbsI GAAGAC 2 cut(s) 736, 3293
Bbv12I GWGCWC 4 cut(s) 2124, 2214, 2259, 3786
BceAI ACGGC 2 cut(s) 59, 523
BciT130I CCWGG 7 cut(s) 439, 591, 600, 768, 820, 1295, 3212
BciVI GTATCC 2 cut(s) 3, 3469
BclI TGATCA 2 cut(s) 1021, 1692
BcuI ACTAGT 4 cut(s) 755, 890, 4030, 4357
BfoI RGCGCY 3 cut(s) 200, 2764, 4306
BfrI CTTAAG 1 cut(s) 1787
BfuAI ACCTGC 2 cut(s) 620, 1251
BfuI GTATCC 2 cut(s) 3, 3469
BglII AGATCT 6 cut(s) 290, 1687, 2023, 2803, 3079, 3866
BlpI GCTNAGC 4 cut(s) 2258, 2412, 3441, 4344
Bme1390I CCNGG 7 cut(s) 439, 591, 600, 768, 820, 1295, 3212
Bme18I GGWCC 3 cut(s) 662, 1240, 1659
BmeRI GACNNNNNGTC 2 cut(s) 465, 511
BmeT110I CYCGRG 3 cut(s) 2586, 3632, 3836
BmgT120I GGNCC 4 cut(s) 435, 662, 1240, 1659
BmiI GGNNCC 7 cut(s) 84, 523, 663, 664, 678, 716, 1259
BmrFI CCNGG 7 cut(s) 439, 591, 600, 768, 820, 1295, 3212
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 6 cut(s) 2854, 3062, 3424, 4063, 4110, 4198
BmuI ACTGGG 1 cut(s) 141
BpiI GAAGAC 2 cut(s) 736, 3293
BpmI CTGGAG 5 cut(s) 612, 802, 1277, 2090, 2726
Bpu1102I GCTNAGC 4 cut(s) 2258, 2412, 3441, 4344
BpuEI CTTGAG 7 cut(s) 1149, 2006, 2690, 3467, 3572, 4145, 4355
Bsa29I ATCGAT 1 cut(s) 48
BsaAI YACGTR 1 cut(s) 1906
BsaI GGTCTC 1 cut(s) 2636
BsaJI CCNNGG 4 cut(s) 237, 276, 301, 438
BsaWI WCCGGW 1 cut(s) 524
BsaXI ACNNNNNCTCC 2 cut(s) 2726, 2756
Bsc4I CCNNNNNNNGG 5 cut(s) 307, 672, 1300, 2854, 2893
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 5 cut(s) 136, 709, 2073, 3477, 3790
Bse3DI GCAATG 6 cut(s) 637, 1193, 1227, 2272, 2726, 2826
BseBI CCWGG 7 cut(s) 439, 591, 600, 768, 820, 1295, 3212
BseCI ATCGAT 1 cut(s) 48
BseDI CCNNGG 4 cut(s) 237, 276, 301, 438
BseLI CCNNNNNNNGG 5 cut(s) 307, 672, 1300, 2854, 2893
BseMI GCAATG 6 cut(s) 637, 1193, 1227, 2272, 2726, 2826
BseNI ACTGG 5 cut(s) 136, 709, 2073, 3477, 3790
BseRI GAGGAG 5 cut(s) 1415, 1934, 2699, 3854, 3857
BseSI GKGCMC 1 cut(s) 2124
BsgI GTGCAG 1 cut(s) 1490
Bsh1236I CGCG 1 cut(s) 1420
BshFI GGCC 3 cut(s) 25, 437, 3177
BshNI GGYRCC 1 cut(s) 82
BshVI ATCGAT 1 cut(s) 48
BsiHKAI GWGCWC 4 cut(s) 2124, 2214, 2259, 3786
BsiHKCI CYCGRG 3 cut(s) 2586, 3632, 3836
BsiSI CCGG 2 cut(s) 26, 525
BslFI GGGAC 6 cut(s) 110, 403, 648, 1830, 3859, 4065
BslI CCNNNNNNNGG 5 cut(s) 307, 672, 1300, 2854, 2893
BsmFI GGGAC 6 cut(s) 110, 403, 648, 1830, 3859, 4065
BsmI GAATGC 1 cut(s) 976
BsnI GGCC 3 cut(s) 25, 437, 3177
Bso31I GGTCTC 1 cut(s) 2636
BsoBI CYCGRG 3 cut(s) 2586, 3632, 3836
Bsp1286I GDGCHC 5 cut(s) 2110, 2124, 2214, 2259, 3786
Bsp1720I GCTNAGC 4 cut(s) 2258, 2412, 3441, 4344
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 4 cut(s) 164, 169, 3999, 4205
BspANI GGCC 3 cut(s) 25, 437, 3177
BspDI ATCGAT 1 cut(s) 48
BspFNI CGCG 1 cut(s) 1420
BspHI TCATGA 2 cut(s) 1668, 1963
BspLI GGNNCC 7 cut(s) 84, 523, 663, 664, 678, 716, 1259
BspMAI CTGCAG 6 cut(s) 631, 922, 1248, 1651, 1813, 3384
BspMI ACCTGC 2 cut(s) 620, 1251
BspPI GGATC 8 cut(s) 289, 516, 529, 559, 671, 684, 830, 3933
BspQI GCTCTTC 2 cut(s) 930, 3793
BspT107I GGYRCC 1 cut(s) 82
BspTI CTTAAG 1 cut(s) 1787
BspTNI GGTCTC 1 cut(s) 2636
BsrDI GCAATG 6 cut(s) 637, 1193, 1227, 2272, 2726, 2826
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 5 cut(s) 136, 709, 2073, 3477, 3790
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 4 cut(s) 237, 276, 301, 438
BssT1I CCWWGG 3 cut(s) 237, 276, 301
Bst2UI CCWGG 7 cut(s) 439, 591, 600, 768, 820, 1295, 3212
Bst4CI ACNGT 6 cut(s) 316, 394, 1505, 2638, 3295, 3322
Bst6I CTCTTC 9 cut(s) 441, 930, 1299, 1488, 1587, 2649, 2907, 3156, 3793
BstAFI CTTAAG 1 cut(s) 1787
BstAPI GCANNNNNTGC 3 cut(s) 1070, 1435, 1477
BstBAI YACGTR 1 cut(s) 1906
BstC8I GCNNGC 4 cut(s) 23, 27, 1038, 1150
BstDSI CCRYGG 1 cut(s) 276
BstENI CCTNNNNNAGG 1 cut(s) 305
BstFNI CGCG 1 cut(s) 1420
BstH2I RGCGCY 3 cut(s) 200, 2764, 4306
BstHHI GCGC 3 cut(s) 199, 2763, 4305
BstNI CCWGG 7 cut(s) 439, 591, 600, 768, 820, 1295, 3212
BstNSI RCATGY 3 cut(s) 947, 1823, 2282
BstSCI CCNGG 7 cut(s) 437, 589, 598, 766, 818, 1293, 3210
BstSLI GKGCMC 1 cut(s) 2124
BstUI CGCG 1 cut(s) 1420
BstV2I GAAGAC 2 cut(s) 736, 3293
BstXI CCANNNNNNTGG 3 cut(s) 93, 244, 4091
Bsu15I ATCGAT 1 cut(s) 48
BsuI GTATCC 2 cut(s) 3, 3469
BsuRI GGCC 3 cut(s) 25, 437, 3177
BsuTUI ATCGAT 1 cut(s) 48
BtgI CCRYGG 1 cut(s) 276
BtsI GCAGTG 4 cut(s) 348, 3572, 3949, 4242
BtsIMutI CAGTG 9 cut(s) 348, 1137, 1842, 2579, 3291, 3572, 3949, 3954, 4242
BveI ACCTGC 2 cut(s) 620, 1251
Cac8I GCNNGC 4 cut(s) 23, 27, 1038, 1150
CaiI CAGNNNCTG 4 cut(s) 1300, 1477, 1489, 3326
CciI TCATGA 2 cut(s) 1668, 1963
CfoI GCGC 3 cut(s) 199, 2763, 4305
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 4 cut(s) 435, 662, 1240, 1659
ClaI ATCGAT 1 cut(s) 48
CseI GACGC 1 cut(s) 1409
Csp6I GTAC 4 cut(s) 531, 1572, 1907, 2724
CspCI CAANNNNNGTGG 2 cut(s) 2555, 2590
CviQI GTAC 4 cut(s) 531, 1572, 1907, 2724
DraIII CACNNNGTG 2 cut(s) 2223, 2873
DriI GACNNNNNGTC 2 cut(s) 465, 511
Eam1104I CTCTTC 9 cut(s) 441, 930, 1299, 1488, 1587, 2649, 2907, 3156, 3793
Eam1105I GACNNNNNGTC 2 cut(s) 465, 511
EarI CTCTTC 9 cut(s) 441, 930, 1299, 1488, 1587, 2649, 2907, 3156, 3793
Ecl136II GAGCTC 2 cut(s) 2212, 3784
Eco130I CCWWGG 3 cut(s) 237, 276, 301
Eco147I AGGCCT 1 cut(s) 3177
Eco24I GRGCYC 3 cut(s) 2110, 2214, 3786
Eco31I GGTCTC 1 cut(s) 2636
Eco47I GGWCC 3 cut(s) 662, 1240, 1659
Eco47III AGCGCT 2 cut(s) 2762, 4304
Eco53kI GAGCTC 2 cut(s) 2212, 3784
Eco57I CTGAAG 9 cut(s) 1229, 2298, 2951, 3018, 3068, 3389, 3629, 3794, 3978
Eco88I CYCGRG 3 cut(s) 2586, 3632, 3836
EcoICRI GAGCTC 2 cut(s) 2212, 3784
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoO109I RGGNCCY 1 cut(s) 662
EcoRI GAATTC 3 cut(s) 850, 2464, 3622
EcoRII CCWGG 7 cut(s) 437, 589, 598, 766, 818, 1293, 3210
EcoT14I CCWWGG 3 cut(s) 237, 276, 301
EcoT38I GRGCYC 3 cut(s) 2110, 2214, 3786
ErhI CCWWGG 3 cut(s) 237, 276, 301
FalI AAGNNNNNCTT 2 cut(s) 2815, 2847
FaqI GGGAC 6 cut(s) 110, 403, 648, 1830, 3859, 4065
FauNDI CATATG 1 cut(s) 1069
FbaI TGATCA 2 cut(s) 1021, 1692
FriOI GRGCYC 3 cut(s) 2110, 2214, 3786
GlaI GCGC 3 cut(s) 198, 2762, 4304
GsuI CTGGAG 5 cut(s) 612, 802, 1277, 2090, 2726
HaeII RGCGCY 3 cut(s) 200, 2764, 4306
HaeIII GGCC 3 cut(s) 25, 437, 3177
HapII CCGG 2 cut(s) 26, 525
HgaI GACGC 1 cut(s) 1409
HhaI GCGC 3 cut(s) 199, 2763, 4305
Hin6I GCGC 3 cut(s) 197, 2761, 4303
HinP1I GCGC 3 cut(s) 197, 2761, 4303
HincII GTYRAC 2 cut(s) 2745, 4099
HindII GTYRAC 2 cut(s) 2745, 4099
HindIII AAGCTT 3 cut(s) 1784, 3443, 3548
HpaII CCGG 2 cut(s) 26, 525
HphI GGTGA 9 cut(s) 415, 719, 867, 1517, 1691, 2206, 2980, 3740, 4000
Hpy166II GTNNAC 6 cut(s) 505, 635, 2122, 2745, 3298, 4099
Hpy8I GTNNAC 6 cut(s) 505, 635, 2122, 2745, 3298, 4099
Hpy99I CGWCG 1 cut(s) 4011
HpyCH4III ACNGT 6 cut(s) 316, 394, 1505, 2638, 3295, 3322
HpyCH4IV ACGT 3 cut(s) 1078, 1905, 2392
HpySE526I ACGT 3 cut(s) 1078, 1905, 2392
HspAI GCGC 3 cut(s) 197, 2761, 4303
KflI GGGWCCC 1 cut(s) 662
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Ksp22I TGATCA 2 cut(s) 1021, 1692
LguI GCTCTTC 2 cut(s) 930, 3793
LmnI GCTCC 8 cut(s) 18, 358, 1054, 1257, 1297, 1729, 2493, 2686
LweI GCATC 6 cut(s) 2854, 3062, 3424, 4063, 4110, 4198
MaeII ACGT 3 cut(s) 1078, 1905, 2392
MaeIII GTNAC 5 cut(s) 925, 1885, 2017, 2625, 2873
MfeI CAATTG 3 cut(s) 141, 1311, 1604
MhlI GDGCHC 5 cut(s) 2110, 2124, 2214, 2259, 3786
MluI ACGCGT 1 cut(s) 1418
MmeI TCCRAC 4 cut(s) 2775, 2911, 3234, 4072
MroNI GCCGGC 1 cut(s) 25
MroXI GAANNNNTTC 3 cut(s) 841, 1543, 3626
MslI CAYNNNNRTG 4 cut(s) 1667, 4008, 4200, 4206
MspA1I CMGCKG 5 cut(s) 164, 180, 1426, 1477, 3346
MspCI CTTAAG 1 cut(s) 1787
MspI CCGG 2 cut(s) 26, 525
MspR9I CCNGG 7 cut(s) 439, 591, 600, 768, 820, 1295, 3212
MunI CAATTG 3 cut(s) 141, 1311, 1604
Mva1269I GAATGC 1 cut(s) 976
MvaI CCWGG 7 cut(s) 439, 591, 600, 768, 820, 1295, 3212
MvnI CGCG 1 cut(s) 1420
NaeI GCCGGC 1 cut(s) 27
NcoI CCATGG 1 cut(s) 276
NdeI CATATG 1 cut(s) 1069
NgoMIV GCCGGC 1 cut(s) 25
NlaIV GGNNCC 7 cut(s) 84, 523, 663, 664, 678, 716, 1259
NmuCI GTSAC 3 cut(s) 1885, 2017, 2873
NspI RCATGY 3 cut(s) 947, 1823, 2282
PaeR7I CTCGAG 3 cut(s) 2586, 3632, 3836
PagI TCATGA 2 cut(s) 1668, 1963
PceI AGGCCT 1 cut(s) 3177
PciSI GCTCTTC 2 cut(s) 930, 3793
PctI GAATGC 1 cut(s) 976
PdiI GCCGGC 1 cut(s) 27
PdmI GAANNNNTTC 3 cut(s) 841, 1543, 3626
PflMI CCANNNNNTGG 1 cut(s) 1300
PfoI TCCNGGA 4 cut(s) 589, 818, 1293, 3210
Ppu21I YACGTR 1 cut(s) 1906
PpuMI RGGWCCY 1 cut(s) 662
PshBI ATTAAT 1 cut(s) 411
PsiI TTATAA 1 cut(s) 4167
Psp124BI GAGCTC 2 cut(s) 2214, 3786
Psp5II RGGWCCY 1 cut(s) 662
Psp6I CCWGG 7 cut(s) 437, 589, 598, 766, 818, 1293, 3210
PspGI CCWGG 7 cut(s) 437, 589, 598, 766, 818, 1293, 3210
PspN4I GGNNCC 7 cut(s) 84, 523, 663, 664, 678, 716, 1259
PspPI GGNCC 4 cut(s) 435, 662, 1240, 1659
PspPPI RGGWCCY 1 cut(s) 662
PspXI VCTCGAGB 1 cut(s) 3836
PstI CTGCAG 6 cut(s) 631, 922, 1248, 1651, 1813, 3384
PstNI CAGNNNCTG 4 cut(s) 1300, 1477, 1489, 3326
PvuII CAGCTG 4 cut(s) 180, 1426, 1477, 3346
RsaI GTAC 4 cut(s) 532, 1573, 1908, 2725
RsaNI GTAC 4 cut(s) 531, 1572, 1907, 2724
RseI CAYNNNNRTG 4 cut(s) 1667, 4008, 4200, 4206
SacI GAGCTC 2 cut(s) 2214, 3786
SapI GCTCTTC 2 cut(s) 930, 3793
Sau96I GGNCC 4 cut(s) 435, 662, 1240, 1659
ScrFI CCNGG 7 cut(s) 439, 591, 600, 768, 820, 1295, 3212
SduI GDGCHC 5 cut(s) 2110, 2124, 2214, 2259, 3786
SfaNI GCATC 6 cut(s) 2854, 3062, 3424, 4063, 4110, 4198
Sfr274I CTCGAG 3 cut(s) 2586, 3632, 3836
SinI GGWCC 3 cut(s) 662, 1240, 1659
SlaI CTCGAG 3 cut(s) 2586, 3632, 3836
SmiMI CAYNNNNRTG 4 cut(s) 1667, 4008, 4200, 4206
SpeI ACTAGT 4 cut(s) 755, 890, 4030, 4357
SseBI AGGCCT 1 cut(s) 3177
SsiI CCGC 4 cut(s) 164, 169, 3999, 4205
SspI AATATT 2 cut(s) 1273, 3142
SstI GAGCTC 2 cut(s) 2214, 3786
StuI AGGCCT 1 cut(s) 3177
StyD4I CCNGG 7 cut(s) 437, 589, 598, 766, 818, 1293, 3210
StyI CCWWGG 3 cut(s) 237, 276, 301
TaaI ACNGT 6 cut(s) 316, 394, 1505, 2638, 3295, 3322
TaiI ACGT 3 cut(s) 1081, 1908, 2395
TatI WGTACW 2 cut(s) 530, 1571
TscAI CASTG 9 cut(s) 355, 1137, 1849, 2579, 3298, 3579, 3949, 3961, 4242
TseFI GTSAC 3 cut(s) 1885, 2017, 2873
Tsp45I GTSAC 3 cut(s) 1885, 2017, 2873
TspGWI ACGGA 5 cut(s) 991, 1276, 1772, 1909, 3574
TspRI CASTG 9 cut(s) 355, 1137, 1849, 2579, 3298, 3579, 3949, 3961, 4242
Van91I CCANNNNNTGG 1 cut(s) 1300
Vha464I CTTAAG 1 cut(s) 1787
VneI GTGCAC 1 cut(s) 2120
VpaK11BI GGWCC 3 cut(s) 662, 1240, 1659
VspI ATTAAT 1 cut(s) 411
XagI CCTNNNNNAGG 1 cut(s) 305
XbaI TCTAGA 4 cut(s) 2404, 3110, 3304, 4273
XceI RCATGY 3 cut(s) 947, 1823, 2282
XcmI CCANNNNNNNNNTGG 3 cut(s) 121, 1234, 1849
XhoI CTCGAG 3 cut(s) 2586, 3632, 3836
XmnI GAANNNNTTC 3 cut(s) 841, 1543, 3626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.