Rh3DG185200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0008872
Physical Location & Seq
Forward (+)
16434410 .. 16441577
7168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG185200.1

Sequence Viewer

Length: 4401 bp
ATGTTTAGGATACACAGGAACAGGCCGGCGAAGTCAGGCGAGAGAATCGATTTCAAGTTCTCTCAGTTCAAAGCCGTTCAGGTGCCGAGAGGATGGGACAAGCTGTATGTGTCCATTGTGTCTGTGGAAACTGGGAAGACAATTGCTAAGTCGAGCAAAGCAGCGGTGCGGAATGGGAGCTGTCAATGGAATGAGGCGCTTTCCGAGTCTATATGGATTTCGCAGGATGATTCTTCCAAGGAGATGGAAGATTCTTTCTTCAAGCTGGTGGTCGCCATGGGATCGGCGAGATCTGGAATCCTTGGAGAGGCTACAGTCAATATGTCGGATTATATAACTTCAAGTTCTACTGCTCCAGTTTCGCTTCCATTGAAAAAGTGCAACTATGAGACAGTTTTACAGGTGAAAATTAACTGCCTAAAGCCAAGAGCAAGGGTCAGGGATGAAGAGTCAAAAGAGACGAGCAGTCATTTGGAAGAACAAAATGGAACTGGTAATGATGTGGACGGCAAATCAGATGGATCCGGTAGTACATTTGGAAGGAGTCTCGGATCTTCCTCCACAAAAGATTTTGGCTTGACCTCAAATCCTGGAGAACCTGGAAGTAGGGGAAGTAGTTTCTCTGCTGCAGGTTCACATTGCAGCTATGAGTCAGCAGAGGGTTCCATAAGAAGGGATCCTATTCTTCTAGGAAGCAACCTTACTGGTGAAGGGAACCTCCTGATTGGAAGACAAGATTCAACCAGCTCTCAAACTAGTTCAATGCCTGGCAATTTTCCAGCCATTTCTTCTCCATCCAATCATTCATCATTTAACTCCAGGATCACAGGTTCAGGAAATCATTCTCAGAATTCTCGCAGAGATATTCCAGAATCACCTTTAAGAATCACTAGTTCTTCTAAAAACCTTCTGGAAACTGCAGAAGTTACTATTGAGGAGCTACATGCAGAAGCTAAGATGTGGGAGAGGAATGCACGGAAACTAATGCTTGATCTGGATATACTGAGGACAGAATTTTCTGATCAGTCAAAAAAGCAGGCAAATTTGAATATGGAGCTTTCAGCAGCATATGCTGAACGTGATAGCTTGAAGAAAGAAGTTGAACACTTAAAAGTGTCATTTGAGAGTTCAGTGATGAGACAAACAGCCAGCGAGGATTTATCTCAAGGTGGAGTGTCTCATATAGAGAAGGCATTACAAGATGAACTGAAGTTTCAAAAAGAATCCATTGCCAATCTGGACCTGCAGTTGAAAAGGAGCCAAGAATCAAATATTGAGCTGGTTTCCGTTCTCCAGGAGCTGGAAGAGACAATGGAAGAACAAAAGATGGAGTTAGAGAATCTATCAGAACTACAATCAAAATTTATTGAAATGGAAAATTCAATCCAAATAACTACAGAGGAGAACAGCAATCTAACGCGTCAGCTGCAAAAATTGCAGGAATCAGAGAACAAGTTGCAAGATATGGTGCAGCAGCTGGAACAGGCTCTGGAAGAGAAAAACCGTGATGTAGAGAAGGGGTCGGGTCTGGAAAAACGAAGCCTTTCGGATATTGAGACAGAATACAGCACATTATTTGATAAAGAAGAAGAGATTATTCAATTGAGAGAAAAGCTGTCTGAGTCTCTCAAAGAAAGCCATTCTGCAGAATTGAGGTCCGTTACCATGAATGGAGGTGAAACAGATCTGATCAGACAAATTGAAGTCTTGAAAGAAAAGTTGCAGGAGCTAGAGACAGATTGTAATGAGCTGACGGATGAAAACCTTGAACTCTTATTCAAGCTTAAGGAAGCAAAGAACAATTCTGCAGGGGGACATGCACCTGTTGATCTTCCCACCACTGAGCTTTTGGTGGATTCTTTTACCAGCTTTGAGTCCAAAATGACTGAAAGGAAATCCCACATACAAAATGCAGAGGAGAATTGCAATAAGATGGTGCTTGGGGAGATTACGAATAATCATGATCTTTCAATTCAAGTGCTTGAGAGTTTAAAAATGGAACTCGAAATTAAAGTCACAGATCTGGGGAAAGAATTGACAGAGAAAAGGTCTGAGATAGCCAAACTGGAGGATAACTTGCTAACCAAAGAAGAAGAAACCAGGGCTCTACGACAGGTGCACAATGAATTAGAGGCTCAGTTTTCCAATCTACAAAGGGAAAAAGTCGAGGTAGAAGAACACATGGAAATCGTGCTAAGAGAAAGTGAGCTCACCACCAAGTGTTTGAATGATTTGCGAAATGATCTAGTGGTGCTCAGCAGCAATGTGGATTCGCATGTTTCTACCAATAAGATACTTGAAAAGAAATCTTCAGAGCTTGAAGCTGATAAATATGAACTGGAACTTCACTTGTCTGAGCTTGAACAACAAAACACACAATTATCGGAACGTATTTCTGCTCTAGAAGCTCAGCTAAGATGTTTGACAGATGAAAATGAGGCTAATCAATTAGAACTAGAGAATTCCAAATCGTATTCTCTAAAGCTCCAAGATGAAATCAGCAGCTTAAAAGTTGAGATGGAATCTGATAAAGTTGAACTGAAACAGAAATTACAGGAATTGCAAAGTCAGTGGTCAGAAGCTCGAGAAGAATGTGAATTTCTTAGAAGGGCAAATCCAAAGTTACAAGCTACCGTGGAGAGCCTCATTGAAGAGTGCAATTCACTTGAGAAATCAAATGAGGAGCTGAGAACACAAAAGCTGGAGTTGCATGAGCAATGTACCTACTTGGAGGCAAGGTTGACTGAATCTCACAAGCGCTTTTCAGATTGCTCGAAAAGAGTTGAAGTGTTGGAACAAGATCTATCTTTGATGATGGAAAGCATTGCCTCAAAAGAGAAAATCCTAAATTCGGAATTAGATGCACTTCGTGATGAAACCATCCAACATAAGGAGAAACTTATTTCGGAAGAGAGCTTGTTGAATAAGATGTATTTGGAGAAGGCAGTTGAAGTTGAGAACCTTCAGCAAGAGGTAGAACAACTCACCAAGCAACTTTCTGAGACTCATAAAGAAAGCGAAGAACTAGCTTCAGGTGCTTTCCACGAAGCATCCAGATTACGAGCTGAGAAGGTAGATCTTCAGTCTGCTCTGCTAGAGGTTCAATCTAGAGCGATACAGACTGAGAATGAACTCAATATTATGAGAGCAGAATACGAAGAGAAGCTACAAGGCCTGTCAGTTGATCTTACTGCTTCTAAAAAAATCCAGGAAACAATGATGGCTGACCATGAAAGGCTATTGAAGTTGTTGGAGAATTACAAGTCAAGTGAAGCAAAATTGAAGACCACTGTGAACAATCTGGAACTAAAACTTACAGTTTCTGATTATGAACAGCAACAGCTGGTGGAAGAATCTACCAATCTGAAGGTTCAACTGCAGAATTTAACTCATTGTCAGGATGAAATGTTGGCTGTTAAGAATGAGCTTGATGCGACTAAGCTTGAGAAAGAGAAATTGGAATTGCTACTGGATACTCAACTACAGAATTTAACTCATTGTCAGGATGAAGTGTTGGCTATAAAGAATGAGCTTGACGCGATCAAGCTTGAGAAAGAGAATTTGGAAGCACTGCTGGATTCCGTGTCTGAGGAATATGAATATCTGAAGGCAGAGAAGAATTCATTCCTCGAGAAGATTTCAACATTGGATATGGTCTTATCTGAATTGGAGGATTGCAAACATAATAAAGCTGTCTTGGAGAGGAAAGTTCTGCGGATGGAAGGTGATCTAATTGCGAAAGAGGCTTTATTTGCACAAGATGCTGAGCTGAAGAATGAGCTCAACCAGTTCAGAAGAGCCAACGAGCAGTATCAACAAAAACTACAGCTACTCGAGGAGGAGAAAGATGAGTGCCTAAGAAGATCTCAGTCCCTTGAACAAGAATTGAAACTAATTAGGGAAGAAAAACAGAACCAGAGGGATCTCAAGTCAAGAAGCAGTGCCAAAGTCACTGAAGATAAGAAGCTCTCAAAAAATGAAATGGTGAAGAATAGCAGTCATCGTCGTGATAATAGAAGGAAGCCACTAGTGAAGAATGGTCAGGATGATAATGGAATTGACAGCCATGATGCAAGTCCTCGTGATGTTGGAGTTGACTCTGGTTTGAAGATTAAGATGCTTGAGGATGAACTTGTTAAGGCATTGGAAGCAAATAACACTTATAAACTTCAACTTGATAGGCTAACCCAACACAACCATGCGGATGCTCGTCAAAATTCAAGGGCCGAAGTAGTGGCAAAAGATAGATTTGAACGCTCGAAATCATCTCTAGAGACAGAATTAAAAGAGATTCGTGAGCGCTACTTGAACATGAGCCTGAGATATGCAGAGGTAGAAGCTGAGCGTGAGGAACTAGTGATGAAGCTCAAGCAATCCAAGAGTGGAAAAAGGTGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1466

Amino Acids

167.35

Weight (kDa)

4.99

Isoelectric Point (pI)

55.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 28 - 140 4.6e-13 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4164
Acc36I ACCTGC 2 cut(s) 620, 1251
AccB1I GGYRCC 1 cut(s) 82
AccB7I CCANNNNNTGG 1 cut(s) 1300
AccII CGCG 2 cut(s) 1420, 3539
AciI CCGC 4 cut(s) 164, 169, 3715, 4202
AclWI GGATC 8 cut(s) 289, 516, 529, 559, 671, 684, 830, 3930
AcuI CTGAAG 9 cut(s) 1229, 2295, 2948, 3015, 3065, 3386, 3626, 3791, 3975
AdeI CACNNNGTG 2 cut(s) 2220, 2870
AfaI GTAC 2 cut(s) 532, 2722
AfeI AGCGCT 2 cut(s) 2759, 4301
AfiI CCNNNNNNNGG 5 cut(s) 307, 672, 1300, 2851, 2890
AflII CTTAAG 1 cut(s) 1784
AflIII ACRYGT 1 cut(s) 1418
AhdI GACNNNNNGTC 1 cut(s) 465
AhlI ACTAGT 4 cut(s) 755, 890, 4027, 4354
AjnI CCWGG 7 cut(s) 589, 598, 766, 818, 1293, 2099, 3207
AjuI GAANNNNNNNTTGG 4 cut(s) 3440, 3472, 3545, 3577
AloI GAACNNNNNNTCC 2 cut(s) 814, 846
Alw21I GWGCWC 4 cut(s) 2121, 2211, 2256, 3783
Alw44I GTGCAC 1 cut(s) 2117
AlwI GGATC 8 cut(s) 289, 516, 529, 559, 671, 684, 830, 3930
AlwNI CAGNNNCTG 4 cut(s) 1300, 1477, 1489, 3323
Ama87I CYCGRG 3 cut(s) 2583, 3629, 3833
Aor51HI AGCGCT 2 cut(s) 2759, 4301
AoxI GGCC 3 cut(s) 23, 3172, 4224
ApaLI GTGCAC 1 cut(s) 2117
ApeKI GCWGC 9 cut(s) 161, 626, 642, 1064, 1426, 1471, 1474, 2259, 2502
ArsI GACNNNNNNTTYG 2 cut(s) 1998, 2030
Asp700I GAANNNNTTC 3 cut(s) 841, 1543, 3623
AspLEI GCGC 3 cut(s) 199, 2760, 4302
AspS9I GGNCC 3 cut(s) 1240, 1656, 4224
AsuHPI GGTGA 8 cut(s) 415, 719, 867, 1688, 2203, 2977, 3737, 3997
AvaI CYCGRG 3 cut(s) 2583, 3629, 3833
AvaII GGWCC 2 cut(s) 1240, 1656
BaeGI GKGCMC 1 cut(s) 2121
BamHI GGATCC 2 cut(s) 521, 676
BanI GGYRCC 1 cut(s) 82
BanII GRGCYC 3 cut(s) 2107, 2211, 3783
BarI GAAGNNNNNNTAC 4 cut(s) 685, 717, 3150, 3182
BauI CACGAG 1 cut(s) 4080
BbsI GAAGAC 3 cut(s) 143, 736, 3290
Bbv12I GWGCWC 4 cut(s) 2121, 2211, 2256, 3783
BbvI GCAGC 9 cut(s) 173, 613, 654, 1076, 1413, 1483, 1486, 2271, 2514
BceAI ACGGC 2 cut(s) 59, 523
BciT130I CCWGG 7 cut(s) 591, 600, 768, 820, 1295, 2101, 3209
BciVI GTATCC 2 cut(s) 3, 3466
BclI TGATCA 2 cut(s) 1021, 1689
BcuI ACTAGT 4 cut(s) 755, 890, 4027, 4354
BfoI RGCGCY 3 cut(s) 200, 2761, 4303
BfrI CTTAAG 1 cut(s) 1784
BfuAI ACCTGC 2 cut(s) 620, 1251
BfuI GTATCC 2 cut(s) 3, 3466
BglII AGATCT 6 cut(s) 290, 1684, 2020, 2800, 3076, 3863
BisI GCNGC 9 cut(s) 162, 627, 643, 1065, 1427, 1472, 1475, 2260, 2503
BlpI GCTNAGC 4 cut(s) 2255, 2409, 3765, 4341
BlsI GCNGC 9 cut(s) 163, 628, 644, 1066, 1428, 1473, 1476, 2261, 2504
Bme1390I CCNGG 7 cut(s) 591, 600, 768, 820, 1295, 2101, 3209
Bme18I GGWCC 2 cut(s) 1240, 1656
BmeRI GACNNNNNGTC 1 cut(s) 465
BmeT110I CYCGRG 3 cut(s) 2583, 3629, 3833
BmgT120I GGNCC 3 cut(s) 1240, 1656, 4224
BmiI GGNNCC 6 cut(s) 84, 523, 664, 678, 716, 1259
BmrFI CCNGG 7 cut(s) 591, 600, 768, 820, 1295, 2101, 3209
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 7 cut(s) 2851, 3059, 3421, 3751, 4060, 4107, 4195
BmuI ACTGGG 1 cut(s) 141
BpiI GAAGAC 3 cut(s) 143, 736, 3290
BpmI CTGGAG 6 cut(s) 339, 612, 802, 1277, 2087, 2723
Bpu1102I GCTNAGC 4 cut(s) 2255, 2409, 3765, 4341
BpuEI CTTGAG 8 cut(s) 1149, 2003, 2687, 3464, 3569, 3911, 4142, 4352
Bsa29I ATCGAT 1 cut(s) 48
BsaJI CCNNGG 5 cut(s) 237, 276, 301, 2100, 2634
BsaWI WCCGGW 1 cut(s) 524
BsaXI ACNNNNNCTCC 2 cut(s) 2723, 2753
Bsc4I CCNNNNNNNGG 5 cut(s) 307, 672, 1300, 2851, 2890
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 8 cut(s) 136, 356, 496, 709, 2070, 2343, 3474, 3787
Bse3DI GCAATG 5 cut(s) 637, 1227, 2269, 2723, 2823
BseBI CCWGG 7 cut(s) 591, 600, 768, 820, 1295, 2101, 3209
BseCI ATCGAT 1 cut(s) 48
BseDI CCNNGG 5 cut(s) 237, 276, 301, 2100, 2634
BseLI CCNNNNNNNGG 5 cut(s) 307, 672, 1300, 2851, 2890
BseMI GCAATG 5 cut(s) 637, 1227, 2269, 2723, 2823
BseNI ACTGG 8 cut(s) 136, 356, 496, 709, 2070, 2343, 3474, 3787
BseRI GAGGAG 6 cut(s) 950, 1415, 1931, 2696, 3851, 3854
BseSI GKGCMC 1 cut(s) 2121
BseXI GCAGC 9 cut(s) 173, 613, 654, 1076, 1413, 1483, 1486, 2271, 2514
BsgI GTGCAG 1 cut(s) 1490
Bsh1236I CGCG 2 cut(s) 1420, 3539
BshFI GGCC 3 cut(s) 25, 3174, 4226
BshNI GGYRCC 1 cut(s) 82
BshVI ATCGAT 1 cut(s) 48
BsiHKAI GWGCWC 4 cut(s) 2121, 2211, 2256, 3783
BsiHKCI CYCGRG 3 cut(s) 2583, 3629, 3833
BsiSI CCGG 2 cut(s) 26, 525
BslFI GGGAC 3 cut(s) 110, 1827, 3856
BslI CCNNNNNNNGG 5 cut(s) 307, 672, 1300, 2851, 2890
BsmBI CGTCTC 1 cut(s) 452
BsmFI GGGAC 3 cut(s) 110, 1827, 3856
BsmI GAATGC 1 cut(s) 976
BsnI GGCC 3 cut(s) 25, 3174, 4226
BsoBI CYCGRG 3 cut(s) 2583, 3629, 3833
Bsp1286I GDGCHC 5 cut(s) 2107, 2121, 2211, 2256, 3783
Bsp1720I GCTNAGC 4 cut(s) 2255, 2409, 3765, 4341
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 4 cut(s) 164, 169, 3715, 4202
BspANI GGCC 3 cut(s) 25, 3174, 4226
BspDI ATCGAT 1 cut(s) 48
BspFNI CGCG 2 cut(s) 1420, 3539
BspHI TCATGA 1 cut(s) 1960
BspLI GGNNCC 6 cut(s) 84, 523, 664, 678, 716, 1259
BspMAI CTGCAG 6 cut(s) 631, 922, 1248, 1648, 1810, 3381
BspMI ACCTGC 2 cut(s) 620, 1251
BspPI GGATC 8 cut(s) 289, 516, 529, 559, 671, 684, 830, 3930
BspQI GCTCTTC 1 cut(s) 3790
BspT107I GGYRCC 1 cut(s) 82
BspTI CTTAAG 1 cut(s) 1784
BsrDI GCAATG 5 cut(s) 637, 1227, 2269, 2723, 2823
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 8 cut(s) 136, 356, 496, 709, 2070, 2343, 3474, 3787
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 5 cut(s) 237, 276, 301, 2100, 2634
BssSI CACGAG 1 cut(s) 4080
BssT1I CCWWGG 3 cut(s) 237, 276, 301
Bst2BI CACGAG 1 cut(s) 4080
Bst2UI CCWGG 7 cut(s) 591, 600, 768, 820, 1295, 2101, 3209
Bst4CI ACNGT 6 cut(s) 316, 394, 1505, 2635, 3292, 3319
Bst6I CTCTTC 8 cut(s) 441, 1299, 1488, 1584, 2646, 2904, 3153, 3790
BstAFI CTTAAG 1 cut(s) 1784
BstAPI GCANNNNNTGC 3 cut(s) 1070, 1435, 3761
BstC8I GCNNGC 3 cut(s) 27, 1038, 1150
BstDSI CCRYGG 2 cut(s) 276, 2634
BstENI CCTNNNNNAGG 1 cut(s) 305
BstFNI CGCG 2 cut(s) 1420, 3539
BstH2I RGCGCY 3 cut(s) 200, 2761, 4303
BstHHI GCGC 3 cut(s) 199, 2760, 4302
BstNI CCWGG 7 cut(s) 591, 600, 768, 820, 1295, 2101, 3209
BstNSI RCATGY 3 cut(s) 947, 1820, 2279
BstSCI CCNGG 7 cut(s) 589, 598, 766, 818, 1293, 2099, 3207
BstSLI GKGCMC 1 cut(s) 2121
BstUI CGCG 2 cut(s) 1420, 3539
BstV1I GCAGC 9 cut(s) 173, 613, 654, 1076, 1413, 1483, 1486, 2271, 2514
BstV2I GAAGAC 3 cut(s) 143, 736, 3290
BstXI CCANNNNNNTGG 1 cut(s) 244
Bsu15I ATCGAT 1 cut(s) 48
BsuI GTATCC 2 cut(s) 3, 3466
BsuRI GGCC 3 cut(s) 25, 3174, 4226
BsuTUI ATCGAT 1 cut(s) 48
BtgI CCRYGG 2 cut(s) 276, 2634
BtsI GCAGTG 2 cut(s) 3569, 3946
BtsIMutI CAGTG 7 cut(s) 1137, 1839, 2576, 3288, 3569, 3946, 3951
BveI ACCTGC 2 cut(s) 620, 1251
Cac8I GCNNGC 3 cut(s) 27, 1038, 1150
CaiI CAGNNNCTG 4 cut(s) 1300, 1477, 1489, 3323
CciI TCATGA 1 cut(s) 1960
CfoI GCGC 3 cut(s) 199, 2760, 4302
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 3 cut(s) 1240, 1656, 4224
ClaI ATCGAT 1 cut(s) 48
CseI GACGC 2 cut(s) 1409, 3545
Csp6I GTAC 2 cut(s) 531, 2721
CspCI CAANNNNNGTGG 2 cut(s) 2552, 2587
CviQI GTAC 2 cut(s) 531, 2721
DraI TTTAAA 1 cut(s) 1992
DraIII CACNNNGTG 2 cut(s) 2220, 2870
DriI GACNNNNNGTC 1 cut(s) 465
Eam1104I CTCTTC 8 cut(s) 441, 1299, 1488, 1584, 2646, 2904, 3153, 3790
Eam1105I GACNNNNNGTC 1 cut(s) 465
EarI CTCTTC 8 cut(s) 441, 1299, 1488, 1584, 2646, 2904, 3153, 3790
Ecl136II GAGCTC 2 cut(s) 2209, 3781
Eco130I CCWWGG 3 cut(s) 237, 276, 301
Eco147I AGGCCT 1 cut(s) 3174
Eco24I GRGCYC 3 cut(s) 2107, 2211, 3783
Eco47I GGWCC 2 cut(s) 1240, 1656
Eco47III AGCGCT 2 cut(s) 2759, 4301
Eco53kI GAGCTC 2 cut(s) 2209, 3781
Eco57I CTGAAG 9 cut(s) 1229, 2295, 2948, 3015, 3065, 3386, 3626, 3791, 3975
Eco88I CYCGRG 3 cut(s) 2583, 3629, 3833
EcoICRI GAGCTC 2 cut(s) 2209, 3781
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoRI GAATTC 3 cut(s) 850, 2461, 3619
EcoRII CCWGG 7 cut(s) 589, 598, 766, 818, 1293, 2099, 3207
EcoT14I CCWWGG 3 cut(s) 237, 276, 301
EcoT38I GRGCYC 3 cut(s) 2107, 2211, 3783
ErhI CCWWGG 3 cut(s) 237, 276, 301
Esp3I CGTCTC 1 cut(s) 452
FaqI GGGAC 3 cut(s) 110, 1827, 3856
FauNDI CATATG 1 cut(s) 1069
FbaI TGATCA 2 cut(s) 1021, 1689
Fnu4HI GCNGC 9 cut(s) 162, 627, 643, 1065, 1427, 1472, 1475, 2260, 2503
FriOI GRGCYC 3 cut(s) 2107, 2211, 3783
Fsp4HI GCNGC 9 cut(s) 162, 627, 643, 1065, 1427, 1472, 1475, 2260, 2503
GlaI GCGC 3 cut(s) 198, 2759, 4301
GluI GCNGC 9 cut(s) 162, 627, 643, 1065, 1427, 1472, 1475, 2260, 2503
GsuI CTGGAG 6 cut(s) 339, 612, 802, 1277, 2087, 2723
HaeII RGCGCY 3 cut(s) 200, 2761, 4303
HaeIII GGCC 3 cut(s) 25, 3174, 4226
HapII CCGG 2 cut(s) 26, 525
HgaI GACGC 2 cut(s) 1409, 3545
HhaI GCGC 3 cut(s) 199, 2760, 4302
Hin6I GCGC 3 cut(s) 197, 2758, 4300
HinP1I GCGC 3 cut(s) 197, 2758, 4300
HincII GTYRAC 2 cut(s) 2742, 4096
HindII GTYRAC 2 cut(s) 2742, 4096
HindIII AAGCTT 3 cut(s) 1781, 3440, 3545
HpaII CCGG 2 cut(s) 26, 525
HphI GGTGA 8 cut(s) 415, 719, 867, 1688, 2203, 2977, 3737, 3997
Hpy166II GTNNAC 6 cut(s) 505, 635, 2119, 2742, 3295, 4096
Hpy8I GTNNAC 6 cut(s) 505, 635, 2119, 2742, 3295, 4096
Hpy99I CGWCG 1 cut(s) 4008
HpyCH4III ACNGT 6 cut(s) 316, 394, 1505, 2635, 3292, 3319
HpyCH4IV ACGT 2 cut(s) 1078, 2389
HpySE526I ACGT 2 cut(s) 1078, 2389
HspAI GCGC 3 cut(s) 197, 2758, 4300
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Ksp22I TGATCA 2 cut(s) 1021, 1689
LguI GCTCTTC 1 cut(s) 3790
LmnI GCTCC 9 cut(s) 177, 358, 937, 1054, 1257, 1297, 1726, 2490, 2683
Lsp1109I GCAGC 9 cut(s) 173, 613, 654, 1076, 1413, 1483, 1486, 2271, 2514
LweI GCATC 7 cut(s) 2851, 3059, 3421, 3751, 4060, 4107, 4195
MaeII ACGT 2 cut(s) 1078, 2389
MaeIII GTNAC 5 cut(s) 925, 1660, 2014, 2622, 3949
MfeI CAATTG 2 cut(s) 141, 1601
MhlI GDGCHC 5 cut(s) 2107, 2121, 2211, 2256, 3783
MluI ACGCGT 1 cut(s) 1418
MlyI GAGTC 8 cut(s) 215, 458, 553, 659, 1631, 1883, 2998, 4091
MmeI TCCRAC 5 cut(s) 306, 2772, 2908, 3231, 4069
MroNI GCCGGC 1 cut(s) 25
MroXI GAANNNNTTC 3 cut(s) 841, 1543, 3623
MslI CAYNNNNRTG 3 cut(s) 4005, 4197, 4203
MspA1I CMGCKG 4 cut(s) 164, 1426, 1477, 3343
MspCI CTTAAG 1 cut(s) 1784
MspI CCGG 2 cut(s) 26, 525
MspR9I CCNGG 7 cut(s) 591, 600, 768, 820, 1295, 2101, 3209
MunI CAATTG 2 cut(s) 141, 1601
Mva1269I GAATGC 1 cut(s) 976
MvaI CCWGG 7 cut(s) 591, 600, 768, 820, 1295, 2101, 3209
MvnI CGCG 2 cut(s) 1420, 3539
NaeI GCCGGC 1 cut(s) 27
NcoI CCATGG 1 cut(s) 276
NdeI CATATG 1 cut(s) 1069
NgoMIV GCCGGC 1 cut(s) 25
NlaIV GGNNCC 6 cut(s) 84, 523, 664, 678, 716, 1259
NmeAIII GCCGAG 1 cut(s) 111
NmuCI GTSAC 2 cut(s) 2014, 3949
NspI RCATGY 3 cut(s) 947, 1820, 2279
PaeR7I CTCGAG 3 cut(s) 2583, 3629, 3833
PagI TCATGA 1 cut(s) 1960
PceI AGGCCT 1 cut(s) 3174
PciSI GCTCTTC 1 cut(s) 3790
PctI GAATGC 1 cut(s) 976
PdiI GCCGGC 1 cut(s) 27
PdmI GAANNNNTTC 3 cut(s) 841, 1543, 3623
PflMI CCANNNNNTGG 1 cut(s) 1300
PfoI TCCNGGA 4 cut(s) 589, 818, 1293, 3207
PkrI GCNGC 9 cut(s) 163, 628, 644, 1066, 1428, 1473, 1476, 2261, 2504
PleI GAGTC 8 cut(s) 214, 457, 552, 658, 1630, 1882, 2998, 4091
PpsI GAGTC 8 cut(s) 214, 457, 552, 658, 1630, 1882, 2998, 4091
PsiI TTATAA 1 cut(s) 4164
Psp124BI GAGCTC 2 cut(s) 2211, 3783
Psp6I CCWGG 7 cut(s) 589, 598, 766, 818, 1293, 2099, 3207
PspGI CCWGG 7 cut(s) 589, 598, 766, 818, 1293, 2099, 3207
PspN4I GGNNCC 6 cut(s) 84, 523, 664, 678, 716, 1259
PspPI GGNCC 3 cut(s) 1240, 1656, 4224
PspXI VCTCGAGB 1 cut(s) 3833
PstI CTGCAG 6 cut(s) 631, 922, 1248, 1648, 1810, 3381
PstNI CAGNNNCTG 4 cut(s) 1300, 1477, 1489, 3323
PvuII CAGCTG 3 cut(s) 1426, 1477, 3343
RsaI GTAC 2 cut(s) 532, 2722
RsaNI GTAC 2 cut(s) 531, 2721
RseI CAYNNNNRTG 3 cut(s) 4005, 4197, 4203
SacI GAGCTC 2 cut(s) 2211, 3783
SapI GCTCTTC 1 cut(s) 3790
SatI GCNGC 9 cut(s) 162, 627, 643, 1065, 1427, 1472, 1475, 2260, 2503
Sau96I GGNCC 3 cut(s) 1240, 1656, 4224
SchI GAGTC 8 cut(s) 215, 458, 553, 659, 1631, 1883, 2998, 4091
ScrFI CCNGG 7 cut(s) 591, 600, 768, 820, 1295, 2101, 3209
SduI GDGCHC 5 cut(s) 2107, 2121, 2211, 2256, 3783
SfaNI GCATC 7 cut(s) 2851, 3059, 3421, 3751, 4060, 4107, 4195
Sfr274I CTCGAG 3 cut(s) 2583, 3629, 3833
SinI GGWCC 2 cut(s) 1240, 1656
SlaI CTCGAG 3 cut(s) 2583, 3629, 3833
SmiMI CAYNNNNRTG 3 cut(s) 4005, 4197, 4203
SpeI ACTAGT 4 cut(s) 755, 890, 4027, 4354
SseBI AGGCCT 1 cut(s) 3174
SsiI CCGC 4 cut(s) 164, 169, 3715, 4202
SspI AATATT 2 cut(s) 1273, 3139
SstI GAGCTC 2 cut(s) 2211, 3783
StuI AGGCCT 1 cut(s) 3174
StyD4I CCNGG 7 cut(s) 589, 598, 766, 818, 1293, 2099, 3207
StyI CCWWGG 3 cut(s) 237, 276, 301
TaaI ACNGT 6 cut(s) 316, 394, 1505, 2635, 3292, 3319
TaiI ACGT 2 cut(s) 1081, 2392
TaqI TCGA 9 cut(s) 48, 152, 2004, 2166, 2584, 2774, 3630, 3834, 4259
TatI WGTACW 1 cut(s) 530
TscAI CASTG 7 cut(s) 1137, 1846, 2576, 3295, 3576, 3946, 3958
TseFI GTSAC 2 cut(s) 2014, 3949
TseI GCWGC 9 cut(s) 161, 626, 642, 1064, 1426, 1471, 1474, 2259, 2502
Tsp45I GTSAC 2 cut(s) 2014, 3949
TspGWI ACGGA 5 cut(s) 991, 1276, 1648, 1769, 3571
TspRI CASTG 7 cut(s) 1137, 1846, 2576, 3295, 3576, 3946, 3958
Van91I CCANNNNNTGG 1 cut(s) 1300
Vha464I CTTAAG 1 cut(s) 1784
VneI GTGCAC 1 cut(s) 2117
VpaK11BI GGWCC 2 cut(s) 1240, 1656
XagI CCTNNNNNAGG 1 cut(s) 305
XbaI TCTAGA 3 cut(s) 2401, 3107, 4270
XceI RCATGY 3 cut(s) 947, 1820, 2279
XcmI CCANNNNNNNNNTGG 3 cut(s) 121, 1234, 1846
XhoI CTCGAG 3 cut(s) 2583, 3629, 3833
XmnI GAANNNNTTC 3 cut(s) 841, 1543, 3623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.