Rw3G015560

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0008872
Physical Location & Seq
Reverse (-)
16107736 .. 16111106
3371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G015560.1

Sequence Viewer

Length: 1887 bp
ATGTTTAGGATACACAGGAACAGGCCGGCGAAGTCAGGCGAGAGAATCGATTTCAAGTTCTCTCAGTTCAAAGCCGTTCAGGTGCCGAGAGGATGGGACAAGCTGTATGTGTCCATTGTGTCTGTGGAAACTGGGAAGACAATTGCTAAGTCGAGCAAAGCGGCGGTGCGGAATGGAAGCTGTCAATGGAGTGAGGCGCTTTCCGAGTCTATATGGATTTCGCAGGATGATTCTTCCAAGGAGATGGAAGATTCTTTCTTCAAGCTGGTGGTTGCCATGGGATCGGCGAGATCTGGAATCCTTGGAGAGGCTACAGTCAATATGTCGGATTATATAACTTCAAGTTCTACTGCTCCAGTTTCGCTTCCATTGAAAAAGTGCAACTATGGGACAGTTTTACAGGTGAAAATTAACTGCCTAAAGCCAAGAGCAAGGGTCAGGGATGAAGAGTCAAAAGAGACAAGCAGTCATTTGGAAGAACAAAATGGAACTGGTAATGATGTGGACGGCAAATCAGATGGATCCGGTAGTACATTTGGAAGGAGTCTCGGATCTTCCTCCACAAAAGATTTTGGCTTGACCTCAAATCCTGGAGAACCTGGAAGTAGGGGAAGTAGTTTCTCTGCTGCAGGTTCACTTTGCAGCTATGAGTCAGCAGAGGGTTCCATAAGAAGGGATCCTATTCTTCTAGGAAGCAACCTTACTGGTGAAGGGAACCTCCTGATTGGAAGACAAGATTCAACCAGCTCTCAAACTAGTTCAATGCCTGGCAATTTTCCAGCCATTTCTTCTCCATCCAATCATTCATCATTTAACTCCAGGATCACAGGTTCAGGAAATCATTCTCAGAATTCTCGCAGAGATATTCCAGAATCACCTTTAAGAACCACTAGTTCTTCTAAAAACCTTCTGGAAACCGCAGAAGTTACTATTGAGGAGCTACATGCAGAAGCTAAGATGTGGGAGAGGAATGCACGGAAACTAATGCTTGATCTGGATATACTGAGGACAGAATTTTCTGATCAGTCAAAAAAGCAGGCAAATTTGAATATGGAGCTTTCAGCAGCATATGCTGAACGTGATAGCTTGAAGAAAGAAGTTGAACAATTAAAAGTGTCATTTGAGAGTTCAGTGATGAGACAAACAGCCAGCGAGGATTTATCTCAAGGTGGAGTGTCTCATATCGAGAAGGCATTACAAGATGAACTGAAGTTTCAAAAAGAATCCATTGCCAATCTGGACCTGCAGTTGAAAAGGAGCCAAGAATCAAATATTGAGCTGGTTTCCGTTCTCCAGGAGCTGGAAGAGACAATGGAAGAACAAAAGATGGAGTTAGAGAATCTATCAGAACTACAATCAAAATTTATTGAAATGGAAAATTCAATCCAAATAACTACAGAGGAGAACAGCAATCTAACGCGTGAGCTGCAAAAATTGCAGGAATCAGAGAACAAGTTGCAAGATATGGTGCAGCAGCTGGAACAGGCTCTGGAAGAGAAAAACCGTGATGTAGAGAAGGGGTCGGGTCTGGAAAAACGAAGCCTTTCGGATATTGAGACAGAATACAGCAGTACATTATTCGATAAAGAAGAAGAGATTATTCAATTGAGAGAAAAGCTGTCTGAGTCTCTCAAAGAAAGCCATTCTGCAGAATTGAGGTCCATTACCATGAATGGAGGTGAAACAGATCTGATCAGACAAATTGAAGTCTTGAAAGAAAAGTTGCAGGAGCTAGAGACAGATTGTAATGAGCTGACGGATGAAAACCTTGAACTCTTATTCAAGCTTAAGGAAGCAAAGAACAATTCTGCAGGGGGACATGCACCTGTTGATCTTCCCACCACTGAGCTTTTGGTGGATTCTTTTACCAGCTTGAGTCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

628

Amino Acids

69.65

Weight (kDa)

4.94

Isoelectric Point (pI)

58.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 28 - 140 6.7e-13 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 620, 1251
AccB1I GGYRCC 1 cut(s) 82
AccB7I CCANNNNNTGG 1 cut(s) 1300
AccII CGCG 1 cut(s) 1420
AciI CCGC 4 cut(s) 161, 164, 169, 918
AclWI GGATC 7 cut(s) 289, 516, 529, 559, 671, 684, 830
AcsI RAATTY 5 cut(s) 850, 1013, 1042, 1361, 1378
AcuI CTGAAG 1 cut(s) 1229
AfaI GTAC 2 cut(s) 532, 1573
AfiI CCNNNNNNNGG 3 cut(s) 307, 672, 1300
AflII CTTAAG 1 cut(s) 1787
AflIII ACRYGT 1 cut(s) 1418
AhdI GACNNNNNGTC 1 cut(s) 465
AhlI ACTAGT 2 cut(s) 755, 890
AjnI CCWGG 5 cut(s) 589, 598, 766, 818, 1293
AloI GAACNNNNNNTCC 2 cut(s) 814, 846
Alw26I GTCTC 8 cut(s) 452, 551, 1132, 1182, 1301, 1550, 1632, 1730
AlwI GGATC 7 cut(s) 289, 516, 529, 559, 671, 684, 830
AlwNI CAGNNNCTG 3 cut(s) 1300, 1477, 1489
AoxI GGCC 1 cut(s) 23
ApeKI GCWGC 6 cut(s) 626, 642, 1064, 1426, 1471, 1474
ApoI RAATTY 5 cut(s) 850, 1013, 1042, 1361, 1378
Asp700I GAANNNNTTC 2 cut(s) 841, 1543
AspLEI GCGC 1 cut(s) 199
AspS9I GGNCC 2 cut(s) 1240, 1659
AsuHPI GGTGA 4 cut(s) 415, 719, 867, 1691
AvaII GGWCC 2 cut(s) 1240, 1659
BamHI GGATCC 2 cut(s) 521, 676
BanI GGYRCC 1 cut(s) 82
BarI GAAGNNNNNNTAC 2 cut(s) 685, 717
BbsI GAAGAC 2 cut(s) 143, 736
BbvI GCAGC 6 cut(s) 613, 654, 1076, 1413, 1483, 1486
BccI CCATC 5 cut(s) 87, 238, 512, 802, 1321
BceAI ACGGC 2 cut(s) 59, 523
BciT130I CCWGG 5 cut(s) 591, 600, 768, 820, 1295
BciVI GTATCC 1 cut(s) 3
BclI TGATCA 2 cut(s) 1021, 1692
BcoDI GTCTC 8 cut(s) 452, 551, 1132, 1182, 1301, 1550, 1632, 1730
BcuI ACTAGT 2 cut(s) 755, 890
BfaI CTAG 4 cut(s) 689, 756, 891, 1733
BfmI CTRYAG 6 cut(s) 312, 627, 1244, 1395, 1647, 1809
BfoI RGCGCY 1 cut(s) 200
BfrI CTTAAG 1 cut(s) 1787
BfuAI ACCTGC 2 cut(s) 620, 1251
BfuI GTATCC 1 cut(s) 3
BglII AGATCT 2 cut(s) 290, 1687
BisI GCNGC 7 cut(s) 162, 627, 643, 1065, 1427, 1472, 1475
BlsI GCNGC 7 cut(s) 163, 628, 644, 1066, 1428, 1473, 1476
Bme1390I CCNGG 5 cut(s) 591, 600, 768, 820, 1295
Bme18I GGWCC 2 cut(s) 1240, 1659
BmeRI GACNNNNNGTC 1 cut(s) 465
BmgT120I GGNCC 2 cut(s) 1240, 1659
BmiI GGNNCC 6 cut(s) 84, 523, 664, 678, 716, 1259
BmrFI CCNGG 5 cut(s) 591, 600, 768, 820, 1295
BmrI ACTGGG 1 cut(s) 141
BmuI ACTGGG 1 cut(s) 141
BpiI GAAGAC 2 cut(s) 143, 736
BpmI CTGGAG 4 cut(s) 339, 612, 802, 1277
BpuEI CTTGAG 1 cut(s) 1149
Bsa29I ATCGAT 1 cut(s) 48
BsaJI CCNNGG 3 cut(s) 237, 276, 301
BsaWI WCCGGW 1 cut(s) 524
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 672, 1300
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 4 cut(s) 136, 356, 496, 709
Bse3DI GCAATG 1 cut(s) 1227
BseBI CCWGG 5 cut(s) 591, 600, 768, 820, 1295
BseCI ATCGAT 1 cut(s) 48
BseDI CCNNGG 3 cut(s) 237, 276, 301
BseGI GGATG 5 cut(s) 98, 232, 448, 794, 1765
BseLI CCNNNNNNNGG 3 cut(s) 307, 672, 1300
BseMI GCAATG 1 cut(s) 1227
BseMII CTCAG 5 cut(s) 77, 860, 995, 1614, 1836
BseNI ACTGG 4 cut(s) 136, 356, 496, 709
BseRI GAGGAG 2 cut(s) 950, 1415
BseXI GCAGC 6 cut(s) 613, 654, 1076, 1413, 1483, 1486
BsgI GTGCAG 1 cut(s) 1490
Bsh1236I CGCG 1 cut(s) 1420
BshFI GGCC 1 cut(s) 25
BshNI GGYRCC 1 cut(s) 82
BshVI ATCGAT 1 cut(s) 48
BsiSI CCGG 2 cut(s) 26, 525
BslFI GGGAC 3 cut(s) 110, 403, 1830
BslI CCNNNNNNNGG 3 cut(s) 307, 672, 1300
BsmAI GTCTC 8 cut(s) 452, 551, 1132, 1182, 1301, 1550, 1632, 1730
BsmFI GGGAC 3 cut(s) 110, 403, 1830
BsmI GAATGC 1 cut(s) 976
BsnI GGCC 1 cut(s) 25
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 4 cut(s) 161, 164, 169, 918
BspANI GGCC 1 cut(s) 25
BspCNI CTCAG 5 cut(s) 76, 859, 996, 1615, 1837
BspDI ATCGAT 1 cut(s) 48
BspFNI CGCG 1 cut(s) 1420
BspLI GGNNCC 6 cut(s) 84, 523, 664, 678, 716, 1259
BspMAI CTGCAG 4 cut(s) 631, 1248, 1651, 1813
BspMI ACCTGC 2 cut(s) 620, 1251
BspPI GGATC 7 cut(s) 289, 516, 529, 559, 671, 684, 830
BspT107I GGYRCC 1 cut(s) 82
BspTI CTTAAG 1 cut(s) 1787
BsrDI GCAATG 1 cut(s) 1227
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 4 cut(s) 136, 356, 496, 709
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 3 cut(s) 237, 276, 301
BssT1I CCWWGG 3 cut(s) 237, 276, 301
Bst2UI CCWGG 5 cut(s) 591, 600, 768, 820, 1295
Bst4CI ACNGT 3 cut(s) 316, 394, 1505
Bst6I CTCTTC 4 cut(s) 441, 1299, 1488, 1587
BstAFI CTTAAG 1 cut(s) 1787
BstAPI GCANNNNNTGC 2 cut(s) 1070, 1435
BstC8I GCNNGC 3 cut(s) 27, 1038, 1150
BstDEI CTNAG 7 cut(s) 63, 147, 846, 954, 1004, 1623, 1845
BstDSI CCRYGG 1 cut(s) 276
BstENI CCTNNNNNAGG 1 cut(s) 305
BstF5I GGATG 5 cut(s) 98, 232, 448, 794, 1765
BstFNI CGCG 1 cut(s) 1420
BstH2I RGCGCY 1 cut(s) 200
BstHHI GCGC 1 cut(s) 199
BstMAI GTCTC 8 cut(s) 452, 551, 1132, 1182, 1301, 1550, 1632, 1730
BstMWI GCNNNNNNNGC 3 cut(s) 1070, 1426, 1435
BstNI CCWGG 5 cut(s) 591, 600, 768, 820, 1295
BstNSI RCATGY 2 cut(s) 947, 1823
BstSCI CCNGG 5 cut(s) 589, 598, 766, 818, 1293
BstSFI CTRYAG 6 cut(s) 312, 627, 1244, 1395, 1647, 1809
BstUI CGCG 1 cut(s) 1420
BstV1I GCAGC 6 cut(s) 613, 654, 1076, 1413, 1483, 1486
BstV2I GAAGAC 2 cut(s) 143, 736
BstX2I RGATCY 5 cut(s) 290, 521, 551, 676, 1687
BstXI CCANNNNNNTGG 1 cut(s) 244
BstYI RGATCY 5 cut(s) 290, 521, 551, 676, 1687
Bsu15I ATCGAT 1 cut(s) 48
BsuI GTATCC 1 cut(s) 3
BsuRI GGCC 1 cut(s) 25
BsuTUI ATCGAT 1 cut(s) 48
BtgI CCRYGG 1 cut(s) 276
BtsCI GGATG 5 cut(s) 98, 232, 448, 794, 1765
BtsIMutI CAGTG 2 cut(s) 1137, 1842
BveI ACCTGC 2 cut(s) 620, 1251
Cac8I GCNNGC 3 cut(s) 27, 1038, 1150
CaiI CAGNNNCTG 3 cut(s) 1300, 1477, 1489
CfoI GCGC 1 cut(s) 199
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 2 cut(s) 1240, 1659
ClaI ATCGAT 1 cut(s) 48
Csp6I GTAC 2 cut(s) 531, 1572
CviAII CATG 4 cut(s) 277, 944, 1669, 1820
CviQI GTAC 2 cut(s) 531, 1572
DdeI CTNAG 7 cut(s) 63, 147, 846, 954, 1004, 1623, 1845
DriI GACNNNNNGTC 1 cut(s) 465
Eam1104I CTCTTC 4 cut(s) 441, 1299, 1488, 1587
Eam1105I GACNNNNNGTC 1 cut(s) 465
EarI CTCTTC 4 cut(s) 441, 1299, 1488, 1587
Eco130I CCWWGG 3 cut(s) 237, 276, 301
Eco47I GGWCC 2 cut(s) 1240, 1659
Eco57I CTGAAG 1 cut(s) 1229
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoRI GAATTC 1 cut(s) 850
EcoRII CCWGG 5 cut(s) 589, 598, 766, 818, 1293
EcoT14I CCWWGG 3 cut(s) 237, 276, 301
ErhI CCWWGG 3 cut(s) 237, 276, 301
FaeI CATG 4 cut(s) 280, 947, 1672, 1823
FaqI GGGAC 3 cut(s) 110, 403, 1830
FatI CATG 4 cut(s) 276, 943, 1668, 1819
FauNDI CATATG 1 cut(s) 1069
FbaI TGATCA 2 cut(s) 1021, 1692
Fnu4HI GCNGC 7 cut(s) 162, 627, 643, 1065, 1427, 1472, 1475
FokI GGATG 5 cut(s) 105, 239, 455, 781, 1772
Fsp4HI GCNGC 7 cut(s) 162, 627, 643, 1065, 1427, 1472, 1475
FspBI CTAG 4 cut(s) 689, 756, 891, 1733
GlaI GCGC 1 cut(s) 198
GluI GCNGC 7 cut(s) 162, 627, 643, 1065, 1427, 1472, 1475
GsuI CTGGAG 4 cut(s) 339, 612, 802, 1277
HaeII RGCGCY 1 cut(s) 200
HaeIII GGCC 1 cut(s) 25
HapII CCGG 2 cut(s) 26, 525
HhaI GCGC 1 cut(s) 199
Hin1II CATG 4 cut(s) 280, 947, 1672, 1823
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HindIII AAGCTT 1 cut(s) 1784
HpaII CCGG 2 cut(s) 26, 525
HphI GGTGA 4 cut(s) 415, 719, 867, 1691
Hpy166II GTNNAC 2 cut(s) 505, 635
Hpy8I GTNNAC 2 cut(s) 505, 635
HpyAV CCTTC 6 cut(s) 534, 666, 704, 917, 1183, 1510
HpyCH4III ACNGT 3 cut(s) 316, 394, 1505
HpyCH4IV ACGT 1 cut(s) 1078
HpyF10VI GCNNNNNNNGC 3 cut(s) 1070, 1426, 1435
HpyF3I CTNAG 7 cut(s) 63, 147, 846, 954, 1004, 1623, 1845
HpySE526I ACGT 1 cut(s) 1078
Hsp92II CATG 4 cut(s) 280, 947, 1672, 1823
HspAI GCGC 1 cut(s) 197
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Ksp22I TGATCA 2 cut(s) 1021, 1692
LmnI GCTCC 6 cut(s) 358, 937, 1054, 1257, 1297, 1729
Lsp1109I GCAGC 6 cut(s) 613, 654, 1076, 1413, 1483, 1486
MaeI CTAG 4 cut(s) 689, 756, 891, 1733
MaeII ACGT 1 cut(s) 1078
MaeIII GTNAC 1 cut(s) 925
MfeI CAATTG 2 cut(s) 141, 1604
MflI RGATCY 5 cut(s) 290, 521, 551, 676, 1687
MluI ACGCGT 1 cut(s) 1418
MlyI GAGTC 6 cut(s) 215, 458, 553, 659, 1634, 1885
MmeI TCCRAC 1 cut(s) 306
MroNI GCCGGC 1 cut(s) 25
MroXI GAANNNNTTC 2 cut(s) 841, 1543
MseI TTAA 5 cut(s) 411, 813, 881, 1109, 1788
MslI CAYNNNNRTG 1 cut(s) 1667
MspA1I CMGCKG 1 cut(s) 1477
MspCI CTTAAG 1 cut(s) 1787
MspI CCGG 2 cut(s) 26, 525
MspR9I CCNGG 5 cut(s) 591, 600, 768, 820, 1295
MunI CAATTG 2 cut(s) 141, 1604
Mva1269I GAATGC 1 cut(s) 976
MvaI CCWGG 5 cut(s) 591, 600, 768, 820, 1295
MvnI CGCG 1 cut(s) 1420
MwoI GCNNNNNNNGC 3 cut(s) 1070, 1426, 1435
NaeI GCCGGC 1 cut(s) 27
NcoI CCATGG 1 cut(s) 276
NdeI CATATG 1 cut(s) 1069
NgoMIV GCCGGC 1 cut(s) 25
NlaIII CATG 4 cut(s) 280, 947, 1672, 1823
NlaIV GGNNCC 6 cut(s) 84, 523, 664, 678, 716, 1259
NmeAIII GCCGAG 1 cut(s) 111
NspI RCATGY 2 cut(s) 947, 1823
PctI GAATGC 1 cut(s) 976
PdiI GCCGGC 1 cut(s) 27
PdmI GAANNNNTTC 2 cut(s) 841, 1543
PflMI CCANNNNNTGG 1 cut(s) 1300
PfoI TCCNGGA 3 cut(s) 589, 818, 1293
PkrI GCNGC 7 cut(s) 163, 628, 644, 1066, 1428, 1473, 1476
PleI GAGTC 6 cut(s) 214, 457, 552, 658, 1633, 1884
PpsI GAGTC 6 cut(s) 214, 457, 552, 658, 1633, 1884
Psp6I CCWGG 5 cut(s) 589, 598, 766, 818, 1293
PspGI CCWGG 5 cut(s) 589, 598, 766, 818, 1293
PspN4I GGNNCC 6 cut(s) 84, 523, 664, 678, 716, 1259
PspPI GGNCC 2 cut(s) 1240, 1659
PstI CTGCAG 4 cut(s) 631, 1248, 1651, 1813
PstNI CAGNNNCTG 3 cut(s) 1300, 1477, 1489
PsuI RGATCY 5 cut(s) 290, 521, 551, 676, 1687
PvuII CAGCTG 1 cut(s) 1477
RsaI GTAC 2 cut(s) 532, 1573
RsaNI GTAC 2 cut(s) 531, 1572
RseI CAYNNNNRTG 1 cut(s) 1667
SaqAI TTAA 5 cut(s) 411, 813, 881, 1109, 1788
SatI GCNGC 7 cut(s) 162, 627, 643, 1065, 1427, 1472, 1475
Sau96I GGNCC 2 cut(s) 1240, 1659
SchI GAGTC 6 cut(s) 215, 458, 553, 659, 1634, 1885
ScrFI CCNGG 5 cut(s) 591, 600, 768, 820, 1295
SfcI CTRYAG 6 cut(s) 312, 627, 1244, 1395, 1647, 1809
SinI GGWCC 2 cut(s) 1240, 1659
SmiMI CAYNNNNRTG 1 cut(s) 1667
SmlI CTYRAG 3 cut(s) 1164, 1787, 1873
SmoI CTYRAG 3 cut(s) 1164, 1787, 1873
SpeI ACTAGT 2 cut(s) 755, 890
SsiI CCGC 4 cut(s) 161, 164, 169, 918
SspI AATATT 1 cut(s) 1273
SspMI CTAG 4 cut(s) 689, 756, 891, 1733
StyD4I CCNGG 5 cut(s) 589, 598, 766, 818, 1293
StyI CCWWGG 3 cut(s) 237, 276, 301
TaaI ACNGT 3 cut(s) 316, 394, 1505
TaiI ACGT 1 cut(s) 1081
TaqI TCGA 4 cut(s) 48, 152, 1185, 1581
TatI WGTACW 2 cut(s) 530, 1571
TauI GCSGC 1 cut(s) 164
Tru1I TTAA 5 cut(s) 411, 813, 881, 1109, 1788
Tru9I TTAA 5 cut(s) 411, 813, 881, 1109, 1788
TscAI CASTG 2 cut(s) 1137, 1849
TseI GCWGC 6 cut(s) 626, 642, 1064, 1426, 1471, 1474
TspDTI ATGAA 5 cut(s) 459, 795, 1218, 1685, 1776
TspGWI ACGGA 3 cut(s) 991, 1276, 1772
TspRI CASTG 2 cut(s) 1137, 1849
Van91I CCANNNNNTGG 1 cut(s) 1300
Vha464I CTTAAG 1 cut(s) 1787
VpaK11BI GGWCC 2 cut(s) 1240, 1659
XagI CCTNNNNNAGG 1 cut(s) 305
XapI RAATTY 5 cut(s) 850, 1013, 1042, 1361, 1378
XceI RCATGY 2 cut(s) 947, 1823
XcmI CCANNNNNNNNNTGG 3 cut(s) 121, 1234, 1849
XmnI GAANNNNTTC 2 cut(s) 841, 1543
XspI CTAG 4 cut(s) 689, 756, 891, 1733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.