MD12G1137800.v1.1

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002726
Physical Location & Seq
Reverse (-)
21544126 .. 21548722
4597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1137800.v1.1.491

Sequence Viewer

Length: 651 bp
ATGGCTGCTCCACCTGCCAGAGCTCGAGCTGATTACGATTACCTCATAAAGCTCCTCCTGATCGGCGACAGCGGTGTGGGTAAGAGTTGCCTTCTTCTGCGTTTCTCTGATGGGTCCTTTACAACTAGTTTCATTACAACCATCGGTATTGATTTCAAGATAAGGACCATAGAGCTTGATGGGAAAAGAATTAAATTGCAAATTTGGGATACTGCTGGTCAAGAACGGTTTCGAACAATTACAACTGCTTACTACCGTGGAGCCATGGGTATTTTGCTCGTGTATGATGTCACTGACGAGTCATCGTTTAACAACATTAGGAATTGGATTCGAAACATAGAACAGCATGCTTCTGACAATGTCAACAAGATCCTTGTGGGTAACAAGGCTGACATGGATGAAAGCAAAAGGGCTGTCCCCACTTCGAAGGGTCAAGCCCTTGCTGACGAATATGGCATCAAATTCTTTGAGACCAGTGCAAAGACAAATTTGAATGTGGAAGAGGTCTTCTTTTCAATAGCTAGGGATATCAAGCAAAGACTTGCCGATACTGACTCGAGGGCTGAGCCTCAGACAATCAAAATTAACCAACCAGACCAGGGAGCTGGGGCTGCTCAAGCTGCCCAAAAATCAGCGTGTTGCGGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04152 ko04530 ko04972 map04144 map04152 map04530 map04972
Pfam Domains
Protein Families
Ras

Protein Analysis

217

Amino Acids

23.88

Weight (kDa)

7.65

Isoelectric Point (pI)

35.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 16 - 147 4.3e-15 ADP-ribosylation factor family
Ras PF00071 17 - 178 2.7e-68 Ras family
Roc PF08477 17 - 131 5.8e-35 Ras of Complex, Roc, domain of DAPkinase
GTP_EFTU PF00009 49 - 173 6.6e-07 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 22
Acc36I ACCTGC 1 cut(s) 22
AciI CCGC 2 cut(s) 72, 642
AclWI GGATC 1 cut(s) 364
AcsI RAATTY 3 cut(s) 201, 461, 487
AfiI CCNNNNNNNGG 1 cut(s) 599
AgsI TTSAA 3 cut(s) 157, 493, 516
AhlI ACTAGT 1 cut(s) 125
AjnI CCWGG 1 cut(s) 597
AluBI AGCT 7 cut(s) 23, 29, 52, 175, 521, 605, 620
AluI AGCT 7 cut(s) 23, 29, 52, 175, 521, 605, 620
Alw21I GWGCWC 1 cut(s) 25
Alw26I GTCTC 1 cut(s) 464
AlwI GGATC 1 cut(s) 364
Ama87I CYCGRG 2 cut(s) 24, 556
ApeKI GCWGC 3 cut(s) 5, 611, 620
ApoI RAATTY 3 cut(s) 201, 461, 487
ArsI GACNNNNNNTTYG 2 cut(s) 399, 431
Asp700I GAANNNNTTC 1 cut(s) 228
AspS9I GGNCC 2 cut(s) 114, 165
AsuII TTCGAA 3 cut(s) 232, 331, 425
AvaI CYCGRG 2 cut(s) 24, 556
AvaII GGWCC 2 cut(s) 114, 165
BanII GRGCYC 1 cut(s) 25
BauI CACGAG 1 cut(s) 278
BbsI GAAGAC 1 cut(s) 499
Bbv12I GWGCWC 1 cut(s) 25
BbvI GCAGC 2 cut(s) 598, 607
BccI CCATC 3 cut(s) 104, 149, 173
BciT130I CCWGG 1 cut(s) 599
BciVI GTATCC 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 464
BcuI ACTAGT 1 cut(s) 125
BfaI CTAG 2 cut(s) 126, 522
BfuAI ACCTGC 1 cut(s) 22
BfuI GTATCC 1 cut(s) 202
BisI GCNGC 3 cut(s) 6, 612, 621
BlpI GCTNAGC 1 cut(s) 564
BlsI GCNGC 3 cut(s) 7, 613, 622
Bme1390I CCNGG 1 cut(s) 599
Bme18I GGWCC 2 cut(s) 114, 165
BmeT110I CYCGRG 2 cut(s) 24, 556
BmgT120I GGNCC 2 cut(s) 114, 165
BmiI GGNNCC 2 cut(s) 115, 262
BmrFI CCNGG 1 cut(s) 599
BmsI GCATC 1 cut(s) 465
BpiI GAAGAC 1 cut(s) 499
Bpu1102I GCTNAGC 1 cut(s) 564
Bpu14I TTCGAA 3 cut(s) 232, 331, 425
BpuEI CTTGAG 1 cut(s) 600
BsaI GGTCTC 1 cut(s) 464
BsaJI CCNNGG 3 cut(s) 256, 264, 598
Bsc4I CCNNNNNNNGG 1 cut(s) 599
Bse1I ACTGG 1 cut(s) 474
BseBI CCWGG 1 cut(s) 599
BseDI CCNNGG 3 cut(s) 256, 264, 598
BseGI GGATG 1 cut(s) 403
BseLI CCNNNNNNNGG 1 cut(s) 599
BseMII CTCAG 2 cut(s) 555, 584
BseNI ACTGG 1 cut(s) 474
BseRI GAGGAG 1 cut(s) 44
BseXI GCAGC 2 cut(s) 598, 607
BseYI CCCAGC 1 cut(s) 605
BsiHKAI GWGCWC 1 cut(s) 25
BsiHKCI CYCGRG 2 cut(s) 24, 556
BslFI GGGAC 1 cut(s) 401
BslI CCNNNNNNNGG 1 cut(s) 599
BsmAI GTCTC 1 cut(s) 464
BsmFI GGGAC 1 cut(s) 401
Bso31I GGTCTC 1 cut(s) 464
BsoBI CYCGRG 2 cut(s) 24, 556
Bsp119I TTCGAA 3 cut(s) 232, 331, 425
Bsp1286I GDGCHC 1 cut(s) 25
Bsp143I GATC 2 cut(s) 60, 369
Bsp1720I GCTNAGC 1 cut(s) 564
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 2 cut(s) 72, 642
BspCNI CTCAG 2 cut(s) 556, 583
BspLI GGNNCC 2 cut(s) 115, 262
BspMI ACCTGC 1 cut(s) 22
BspPI GGATC 1 cut(s) 364
BspT104I TTCGAA 3 cut(s) 232, 331, 425
BspTNI GGTCTC 1 cut(s) 464
BsrI ACTGG 1 cut(s) 474
BssECI CCNNGG 3 cut(s) 256, 264, 598
BssMI GATC 2 cut(s) 60, 369
BssSI CACGAG 1 cut(s) 278
BssT1I CCWWGG 1 cut(s) 264
Bst2BI CACGAG 1 cut(s) 278
Bst2UI CCWGG 1 cut(s) 599
Bst4CI ACNGT 2 cut(s) 228, 257
Bst6I CTCTTC 1 cut(s) 495
BstBI TTCGAA 3 cut(s) 232, 331, 425
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 3 cut(s) 564, 570, 648
BstDSI CCRYGG 2 cut(s) 256, 264
BstF5I GGATG 1 cut(s) 403
BstKTI GATC 2 cut(s) 63, 372
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 2 cut(s) 60, 369
BstMWI GCNNNNNNNGC 4 cut(s) 14, 611, 617, 620
BstNI CCWGG 1 cut(s) 599
BstNSI RCATGY 1 cut(s) 350
BstSCI CCNGG 1 cut(s) 597
BstV1I GCAGC 2 cut(s) 598, 607
BstV2I GAAGAC 1 cut(s) 499
BstX2I RGATCY 1 cut(s) 369
BstXI CCANNNNNNTGG 1 cut(s) 605
BstYI RGATCY 1 cut(s) 369
BsuI GTATCC 1 cut(s) 202
BtgI CCRYGG 2 cut(s) 256, 264
BtsCI GGATG 1 cut(s) 403
BtsIMutI CAGTG 2 cut(s) 291, 481
BveI ACCTGC 1 cut(s) 22
Cac8I GCNNGC 1 cut(s) 348
Cfr13I GGNCC 2 cut(s) 114, 165
CviAII CATG 3 cut(s) 265, 347, 394
DdeI CTNAG 3 cut(s) 564, 570, 648
DpnI GATC 2 cut(s) 62, 371
DpnII GATC 2 cut(s) 60, 369
Eam1104I CTCTTC 1 cut(s) 495
EarI CTCTTC 1 cut(s) 495
Ecl136II GAGCTC 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 25
Eco31I GGTCTC 1 cut(s) 464
Eco32I GATATC 1 cut(s) 529
Eco47I GGWCC 2 cut(s) 114, 165
Eco53kI GAGCTC 1 cut(s) 23
Eco88I CYCGRG 2 cut(s) 24, 556
EcoICRI GAGCTC 1 cut(s) 23
EcoO109I RGGNCCY 1 cut(s) 114
EcoRII CCWGG 1 cut(s) 597
EcoRV GATATC 1 cut(s) 529
EcoT14I CCWWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 25
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 3 cut(s) 268, 350, 397
FaiI YATR 8 cut(s) 47, 170, 266, 285, 338, 348, 395, 453
FaqI GGGAC 1 cut(s) 401
FatI CATG 3 cut(s) 264, 346, 393
Fnu4HI GCNGC 3 cut(s) 6, 612, 621
FokI GGATG 1 cut(s) 410
FriOI GRGCYC 1 cut(s) 25
Fsp4HI GCNGC 3 cut(s) 6, 612, 621
FspBI CTAG 2 cut(s) 126, 522
GluI GCNGC 3 cut(s) 6, 612, 621
GsaI CCCAGC 1 cut(s) 609
Hin1II CATG 3 cut(s) 268, 350, 397
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 3 cut(s) 299, 328, 554
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 3 cut(s) 109, 355, 573
Hpy188III TCNNGA 3 cut(s) 58, 157, 221
Hpy8I GTNNAC 1 cut(s) 364
HpyAV CCTTC 2 cut(s) 101, 421
HpyCH4III ACNGT 2 cut(s) 228, 257
HpyCH4V TGCA 2 cut(s) 199, 479
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 611, 617, 620
HpyF3I CTNAG 3 cut(s) 564, 570, 648
Hsp92II CATG 3 cut(s) 268, 350, 397
Kzo9I GATC 2 cut(s) 60, 369
LmnI GCTCC 4 cut(s) 13, 57, 260, 602
LpnPI CCDG 9 cut(s) 27, 31, 71, 201, 487, 584, 591, 606, 611
Lsp1109I GCAGC 2 cut(s) 598, 607
LweI GCATC 1 cut(s) 465
MaeI CTAG 2 cut(s) 126, 522
MaeIII GTNAC 2 cut(s) 289, 380
MalI GATC 2 cut(s) 62, 371
MboI GATC 2 cut(s) 60, 369
MboII GAAGA 3 cut(s) 86, 499, 512
MflI RGATCY 1 cut(s) 369
MhlI GDGCHC 1 cut(s) 25
MluCI AATT 8 cut(s) 189, 194, 201, 237, 322, 461, 487, 582
MlyI GAGTC 2 cut(s) 308, 548
MnlI CCTC 5 cut(s) 53, 65, 496, 552, 579
MroXI GAANNNNTTC 1 cut(s) 228
MseI TTAA 3 cut(s) 192, 309, 585
MspA1I CMGCKG 1 cut(s) 72
MspR9I CCNGG 1 cut(s) 599
MvaI CCWGG 1 cut(s) 599
MwoI GCNNNNNNNGC 4 cut(s) 14, 611, 617, 620
NcoI CCATGG 1 cut(s) 264
NdeII GATC 2 cut(s) 60, 369
NlaIII CATG 3 cut(s) 268, 350, 397
NlaIV GGNNCC 2 cut(s) 115, 262
NmuCI GTSAC 1 cut(s) 289
NspI RCATGY 1 cut(s) 350
NspV TTCGAA 3 cut(s) 232, 331, 425
PaeI GCATGC 1 cut(s) 350
PaeR7I CTCGAG 2 cut(s) 24, 556
PaqCI CACCTGC 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 228
PfeI GAWTC 1 cut(s) 328
PflFI GACNNNGTC 1 cut(s) 359
PkrI GCNGC 3 cut(s) 7, 613, 622
PleI GAGTC 2 cut(s) 307, 548
PpsI GAGTC 2 cut(s) 307, 548
PpuMI RGGWCCY 1 cut(s) 114
Psp124BI GAGCTC 1 cut(s) 25
Psp5II RGGWCCY 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 597
PspFI CCCAGC 1 cut(s) 605
PspGI CCWGG 1 cut(s) 597
PspN4I GGNNCC 2 cut(s) 115, 262
PspPI GGNCC 2 cut(s) 114, 165
PspPPI RGGWCCY 1 cut(s) 114
PspXI VCTCGAGB 2 cut(s) 24, 556
PsuI RGATCY 1 cut(s) 369
PsyI GACNNNGTC 1 cut(s) 359
SacI GAGCTC 1 cut(s) 25
SaqAI TTAA 3 cut(s) 192, 309, 585
SatI GCNGC 3 cut(s) 6, 612, 621
Sau3AI GATC 2 cut(s) 60, 369
Sau96I GGNCC 2 cut(s) 114, 165
SchI GAGTC 2 cut(s) 308, 548
ScrFI CCNGG 1 cut(s) 599
SduI GDGCHC 1 cut(s) 25
SfaNI GCATC 1 cut(s) 465
Sfr274I CTCGAG 2 cut(s) 24, 556
SfuI TTCGAA 3 cut(s) 232, 331, 425
SinI GGWCC 2 cut(s) 114, 165
SlaI CTCGAG 2 cut(s) 24, 556
SmlI CTYRAG 3 cut(s) 24, 556, 615
SmoI CTYRAG 3 cut(s) 24, 556, 615
SpeI ACTAGT 1 cut(s) 125
SphI GCATGC 1 cut(s) 350
Sse9I AATT 8 cut(s) 189, 194, 201, 237, 322, 461, 487, 582
SsiI CCGC 2 cut(s) 72, 642
SspMI CTAG 2 cut(s) 126, 522
SstI GAGCTC 1 cut(s) 25
StyD4I CCNGG 1 cut(s) 597
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 2 cut(s) 228, 257
TaqI TCGA 5 cut(s) 25, 232, 331, 425, 557
TasI AATT 8 cut(s) 189, 194, 201, 237, 322, 461, 487, 582
TfiI GAWTC 1 cut(s) 328
Tru1I TTAA 3 cut(s) 192, 309, 585
Tru9I TTAA 3 cut(s) 192, 309, 585
TscAI CASTG 2 cut(s) 298, 481
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 3 cut(s) 5, 611, 620
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 2 cut(s) 121, 414
TspRI CASTG 2 cut(s) 298, 481
Tth111I GACNNNGTC 1 cut(s) 359
VpaK11BI GGWCC 2 cut(s) 114, 165
XapI RAATTY 3 cut(s) 201, 461, 487
XceI RCATGY 1 cut(s) 350
XhoI CTCGAG 2 cut(s) 24, 556
XmnI GAANNNNTTC 1 cut(s) 228
XspI CTAG 2 cut(s) 126, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.