MD12G1183400.v1.1

LURP-one-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006413
Physical Location & Seq
Forward (+)
26352748 .. 26354069
1322 bp
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UTR
Exon/CDS
Intron
MD12G1183400.v1.1.491

Sequence Viewer

Length: 633 bp
ATGTCGTTTTCCGTGCCTGTACCTTCAGCTGGACCAAGCGCTCCGCCTCTGTCGAACCCTGTGGTTGTGGTCAGCTCACAGTTCCTAGCACCGTACCCCGTGGATCTAGTCATTAGCGAGAAGCTCATGACCCTTAAGGAGGGTTCCTTCGCCGTCTCTGATGTTAATGGCGACATCATGTTCAGCGTTAAGGGTTCCGTTTTCAGCCTCCATGATAAACGCGTATTAGTTGACAGCGCCGGTACTCCTATTGTCACATTTCGACAGAAGATACTGACGACTCATAGGAGATGGCAAGTATACAAAGGAGATAGCAAAGATCTACTTTTCAGTGCCAAAAAGTCGGGGGTTGTTCAATTGAAGACTGAATTAGATGTGTTCTTGGCTTCTAACACAAAAGAAGACACCCATGATTTCAAGGTCAAAGGAAGTTTTAAGGAAAGATCATGCACTGTATACACCAGAGAGAACACTATCATTGCACAAATGCATAAGCAACTCGGCCTTAAAAGCAAAGTTTTAGGGCAAGATTCGTTTTCAGTGACGGTGTACCCTCAGGTGGATTTCGGATTTATAGTTGCTGTGGTGGTGGTTCTTCATGAGATTAATCAAGATCGAAAAGGGGAAGACTAG

Protein Analysis

211

Amino Acids

23.17

Weight (kDa)

8.89

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOR PF04525 34 - 199 6.5e-46 LURP-one-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 300, 456
AccII CGCG 1 cut(s) 222
AciI CCGC 1 cut(s) 44
AclWI GGATC 1 cut(s) 111
AcuI CTGAAG 1 cut(s) 9
AfaI GTAC 4 cut(s) 21, 95, 244, 551
AfeI AGCGCT 1 cut(s) 40
AfiI CCNNNNNNNGG 3 cut(s) 29, 139, 559
AflII CTTAAG 1 cut(s) 134
AflIII ACRYGT 1 cut(s) 220
AgsI TTSAA 3 cut(s) 356, 361, 418
AluBI AGCT 3 cut(s) 29, 75, 124
AluI AGCT 3 cut(s) 29, 75, 124
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 1 cut(s) 111
Aor51HI AGCGCT 1 cut(s) 40
AoxI GGCC 1 cut(s) 502
AseI ATTAAT 1 cut(s) 606
AspLEI GCGC 2 cut(s) 41, 239
AspS9I GGNCC 1 cut(s) 32
AvaII GGWCC 1 cut(s) 32
AxyI CCTNAGG 1 cut(s) 555
BbsI GAAGAC 2 cut(s) 368, 408
BccI CCATC 1 cut(s) 285
BceAI ACGGC 1 cut(s) 137
BcoDI GTCTC 1 cut(s) 160
BfaI CTAG 3 cut(s) 86, 107, 631
BfoI RGCGCY 2 cut(s) 42, 240
BfrI CTTAAG 1 cut(s) 134
BglII AGATCT 1 cut(s) 319
Bme18I GGWCC 1 cut(s) 32
BmgT120I GGNCC 1 cut(s) 32
BmiI GGNNCC 2 cut(s) 145, 196
BpiI GAAGAC 2 cut(s) 368, 408
BsaJI CCNNGG 1 cut(s) 99
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 139, 559
Bse118I RCCGGY 1 cut(s) 239
Bse21I CCTNAGG 1 cut(s) 555
Bse3DI GCAATG 1 cut(s) 477
BseDI CCNNGG 1 cut(s) 99
BseLI CCNNNNNNNGG 3 cut(s) 29, 139, 559
BseMI GCAATG 1 cut(s) 477
BseMII CTCAG 1 cut(s) 569
Bsh1236I CGCG 1 cut(s) 222
BshFI GGCC 1 cut(s) 504
BsiSI CCGG 1 cut(s) 240
BslI CCNNNNNNNGG 3 cut(s) 29, 139, 559
BsmAI GTCTC 1 cut(s) 160
BsmBI CGTCTC 1 cut(s) 160
BsnI GGCC 1 cut(s) 504
Bsp143I GATC 4 cut(s) 103, 319, 443, 613
BspACI CCGC 1 cut(s) 44
BspANI GGCC 1 cut(s) 504
BspCNI CTCAG 1 cut(s) 568
BspFNI CGCG 1 cut(s) 222
BspHI TCATGA 2 cut(s) 126, 598
BspLI GGNNCC 2 cut(s) 145, 196
BspPI GGATC 1 cut(s) 111
BspTI CTTAAG 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 477
BsrFI RCCGGY 1 cut(s) 239
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 4 cut(s) 103, 319, 443, 613
BssNAI GTATAC 2 cut(s) 301, 457
Bst1107I GTATAC 2 cut(s) 301, 457
Bst4CI ACNGT 4 cut(s) 81, 93, 454, 547
BstAFI CTTAAG 1 cut(s) 134
BstDEI CTNAG 1 cut(s) 555
BstDSI CCRYGG 1 cut(s) 99
BstENI CCTNNNNNAGG 1 cut(s) 137
BstFNI CGCG 1 cut(s) 222
BstH2I RGCGCY 2 cut(s) 42, 240
BstHHI GCGC 2 cut(s) 41, 239
BstKTI GATC 4 cut(s) 106, 322, 446, 616
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 4 cut(s) 103, 319, 443, 613
BstMWI GCNNNNNNNGC 1 cut(s) 510
BstUI CGCG 1 cut(s) 222
BstV2I GAAGAC 2 cut(s) 368, 408
BstX2I RGATCY 2 cut(s) 103, 319
BstYI RGATCY 2 cut(s) 103, 319
BstZ17I GTATAC 2 cut(s) 301, 457
Bsu36I CCTNAGG 1 cut(s) 555
BsuRI GGCC 1 cut(s) 504
BtgI CCRYGG 1 cut(s) 99
BtsIMutI CAGTG 3 cut(s) 337, 450, 546
CciI TCATGA 2 cut(s) 126, 598
CfoI GCGC 2 cut(s) 41, 239
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 1 cut(s) 32
Csp6I GTAC 4 cut(s) 20, 94, 243, 550
CviAII CATG 6 cut(s) 127, 178, 212, 410, 447, 599
CviJI RGCY 6 cut(s) 29, 75, 124, 207, 386, 504
CviKI_1 RGCY 6 cut(s) 29, 75, 124, 207, 386, 504
CviQI GTAC 4 cut(s) 20, 94, 243, 550
DdeI CTNAG 1 cut(s) 555
DpnI GATC 4 cut(s) 105, 321, 445, 615
DpnII GATC 4 cut(s) 103, 319, 443, 613
EciI GGCGGA 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 32
Eco47III AGCGCT 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 9
Eco81I CCTNAGG 1 cut(s) 555
EcoNI CCTNNNNNAGG 1 cut(s) 137
EcoT22I ATGCAT 1 cut(s) 492
Esp3I CGTCTC 1 cut(s) 160
FaeI CATG 6 cut(s) 130, 181, 215, 413, 450, 602
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FatI CATG 6 cut(s) 126, 177, 211, 409, 446, 598
FblI GTMKAC 2 cut(s) 300, 456
FspBI CTAG 3 cut(s) 86, 107, 631
GlaI GCGC 2 cut(s) 40, 238
HaeII RGCGCY 2 cut(s) 42, 240
HaeIII GGCC 1 cut(s) 504
HapII CCGG 1 cut(s) 240
HhaI GCGC 2 cut(s) 41, 239
Hin1II CATG 6 cut(s) 130, 181, 215, 413, 450, 602
Hin6I GCGC 2 cut(s) 39, 237
HinP1I GCGC 2 cut(s) 39, 237
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HinfI GANTC 2 cut(s) 280, 530
HpaII CCGG 1 cut(s) 240
Hpy166II GTNNAC 4 cut(s) 232, 301, 457, 550
Hpy188I TCNGA 2 cut(s) 160, 569
Hpy188III TCNNGA 3 cut(s) 127, 599, 611
Hpy8I GTNNAC 4 cut(s) 232, 301, 457, 550
HpyAV CCTTC 2 cut(s) 33, 157
HpyCH4III ACNGT 4 cut(s) 81, 93, 454, 547
HpyCH4V TGCA 3 cut(s) 450, 482, 490
HpyF10VI GCNNNNNNNGC 1 cut(s) 510
HpyF3I CTNAG 1 cut(s) 555
Hsp92II CATG 6 cut(s) 130, 181, 215, 413, 450, 602
HspAI GCGC 2 cut(s) 39, 237
Kzo9I GATC 4 cut(s) 103, 319, 443, 613
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 6 cut(s) 15, 30, 72, 253, 475, 542
MaeI CTAG 3 cut(s) 86, 107, 631
MaeIII GTNAC 2 cut(s) 253, 541
MalI GATC 4 cut(s) 105, 321, 445, 615
MboI GATC 4 cut(s) 103, 319, 443, 613
MboII GAAGA 4 cut(s) 280, 373, 413, 587
MfeI CAATTG 1 cut(s) 356
MflI RGATCY 2 cut(s) 103, 319
MluCI AATT 2 cut(s) 356, 368
MluI ACGCGT 1 cut(s) 220
MlyI GAGTC 1 cut(s) 274
MnlI CCTC 4 cut(s) 57, 133, 218, 564
Mph1103I ATGCAT 1 cut(s) 492
MseI TTAA 6 cut(s) 135, 165, 189, 435, 507, 606
MspA1I CMGCKG 1 cut(s) 29
MspCI CTTAAG 1 cut(s) 134
MspI CCGG 1 cut(s) 240
MunI CAATTG 1 cut(s) 356
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 1 cut(s) 510
NdeII GATC 4 cut(s) 103, 319, 443, 613
NlaIII CATG 6 cut(s) 130, 181, 215, 413, 450, 602
NlaIV GGNNCC 2 cut(s) 145, 196
NmeAIII GCCGAG 1 cut(s) 480
NmuCI GTSAC 2 cut(s) 253, 541
NsiI ATGCAT 1 cut(s) 492
PagI TCATGA 2 cut(s) 126, 598
PfeI GAWTC 1 cut(s) 530
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
PshBI ATTAAT 1 cut(s) 606
PspN4I GGNNCC 2 cut(s) 145, 196
PspPI GGNCC 1 cut(s) 32
PsuI RGATCY 2 cut(s) 103, 319
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 4 cut(s) 21, 95, 244, 551
RsaNI GTAC 4 cut(s) 20, 94, 243, 550
SaqAI TTAA 6 cut(s) 135, 165, 189, 435, 507, 606
Sau3AI GATC 4 cut(s) 103, 319, 443, 613
Sau96I GGNCC 1 cut(s) 32
SchI GAGTC 1 cut(s) 274
SetI ASST 6 cut(s) 25, 31, 77, 126, 423, 561
SinI GGWCC 1 cut(s) 32
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 2 cut(s) 356, 368
SsiI CCGC 1 cut(s) 44
SspMI CTAG 3 cut(s) 86, 107, 631
TaaI ACNGT 4 cut(s) 81, 93, 454, 547
TaqI TCGA 3 cut(s) 53, 262, 616
TasI AATT 2 cut(s) 356, 368
TfiI GAWTC 1 cut(s) 530
Tru1I TTAA 6 cut(s) 135, 165, 189, 435, 507, 606
Tru9I TTAA 6 cut(s) 135, 165, 189, 435, 507, 606
TscAI CASTG 3 cut(s) 337, 457, 546
TseFI GTSAC 2 cut(s) 253, 541
Tsp45I GTSAC 2 cut(s) 253, 541
TspDTI ATGAA 1 cut(s) 587
TspGWI ACGGA 1 cut(s) 187
TspRI CASTG 3 cut(s) 337, 457, 546
Vha464I CTTAAG 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 32
VspI ATTAAT 1 cut(s) 606
XagI CCTNNNNNAGG 1 cut(s) 137
XmiI GTMKAC 2 cut(s) 300, 456
XspI CTAG 3 cut(s) 86, 107, 631
Zsp2I ATGCAT 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.