MD12G1185800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011169
Physical Location & Seq
Reverse (-)
26744808 .. 26746907
2100 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1185800.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGGCTATAATGCGCGCCAAGGGCCGCTTAACCACGGCGGACCCGCCTCCTTCTCCAATCCCAACCGGTAGAGGCTCCCGCTCTGCGGCCAACCAGAACTTCATCCAATTCCTCGACAAGTCCCTGCAGATCCCGGAGCTCACCGCGTTGCCGCCTTCGGGCTCCGGCACACGTCACCAACTCCCTGCTGTCGTCGACTTCCGATCGCTCTCCGCCGAGTCCTTCGCCCGGCTTCTCGCCTCGGCCAAGCAGTTCGGCGCGTTTCGGATCAGCAATCACGGGATTTCGGCCGAGGAGTTGGGATCCGTGGTTTGGGAAGCCGAGTCGGTTTCCCGAAACGACGGAGTAATCGGAAAACGATTCATTGAACGCATTGGGAATCGCGAGGACATTAAATGGCTTCCGCAGGTATCGGATGACAAAAGATATCGGGACTTATGCAACAATATGGAGAAGGTTGCAAGAAAAGTTCAGGCAATTGCTGAGCAGCTGAGCCAAGTTTTTAATGGAAATGCAGAAAAGCAATTTGAGACGATTGGAAGTGAAATGGGAAATGTAAAGTTATTAAGATACAATCACCAAGATCATTCCATGGAGCAAAACCCTAGTAAGAATTTTTTACAGAATGAGATAAATAATGGCAGTCACATTCACATGCGTGAGAGCGATGATGATCATGCTTTGTGCCTTCACCTTCCGCTTGAGCACTCTCAGTTTAATGTCCGATCAGAGGGAGGCTCTGCATTGCTGTTTGATGCAGGGCCAGAGACTCTAGTCGTCACTGTTGGAAAGCAACTAGAGGGATTTAAATGTGTTTCAGGGGAAATGATCTTTGTCCCAGACAGTAGAAGCCAAGCCTCTTTCTCCATACAACTCAAAGTTCCATTGGTTTCAAATTCTAGAAAAATTTGTAAGACAGTTTCCATTGCTGAACAAATCCTCATTGCAGTCATTCTATATGTACTGTACACATTTGTTGTATTTACATACACACACATCATCACTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

337

Amino Acids

37.3

Weight (kDa)

7.16

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011169)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38500
fragaria_vesca FvH4_6g10130
malus_domestica MD12G1185800.v1.1
prunus_persica Prupe.6G291400_v2.0.a1
pyrus_communis pycom12g17450
rosa_chinensis RchiOBHm_Chr3g0460831
rosa_laevigata RLG00000018120 RLG00000024976
rosa_multiflora Rmu_sc0007678.1_g000011
rosa_roxburghii Rroxscaffold_5G00381390 Rroxscaffold_6G00419180
rosa_rugosa Rorug02G0156100 Rorug03G0046800
rosa_samantha Rh1AG194200 Rh3AG103900 Rh3BG107500 Rh3CG109400 Rh3DG109300 Rh7DG284200
rosa_wichuraiana Rw3G008840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 397
AccBSI CCGCTC 1 cut(s) 81
AccI GTMKAC 1 cut(s) 195
AccII CGCG 4 cut(s) 15, 146, 260, 384
AclWI GGATC 4 cut(s) 124, 275, 297, 310
AcoI YGGCCR 3 cut(s) 87, 243, 288
AcsI RAATTY 3 cut(s) 613, 895, 906
AfaI GTAC 2 cut(s) 963, 968
AfiI CCNNNNNNNGG 5 cut(s) 85, 158, 228, 312, 730
AflIII ACRYGT 1 cut(s) 170
AgeI ACCGGT 1 cut(s) 65
AgsI TTSAA 2 cut(s) 368, 894
AjiI CACGTC 1 cut(s) 173
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
AleI CACNNNNGTG 1 cut(s) 657
AluBI AGCT 2 cut(s) 139, 490
AluI AGCT 2 cut(s) 139, 490
Alw21I GWGCWC 2 cut(s) 141, 708
Alw26I GTCTC 2 cut(s) 524, 761
AlwI GGATC 4 cut(s) 124, 275, 297, 310
AoxI GGCC 5 cut(s) 22, 87, 243, 288, 761
ApeKI GCWGC 1 cut(s) 487
ApoI RAATTY 3 cut(s) 613, 895, 906
AsiGI ACCGGT 1 cut(s) 65
AspLEI GCGC 3 cut(s) 15, 17, 260
AspS9I GGNCC 3 cut(s) 22, 40, 761
AsuC2I CCSGG 2 cut(s) 134, 229
AsuHPI GGTGA 4 cut(s) 133, 167, 569, 683
AvaII GGWCC 1 cut(s) 40
BamHI GGATCC 1 cut(s) 302
BanII GRGCYC 2 cut(s) 141, 164
Bbv12I GWGCWC 2 cut(s) 141, 708
BbvI GCAGC 1 cut(s) 499
BceAI ACGGC 1 cut(s) 51
BclI TGATCA 1 cut(s) 673
BcnI CCSGG 2 cut(s) 134, 229
BcoDI GTCTC 2 cut(s) 524, 761
BfaI CTAG 4 cut(s) 606, 773, 797, 900
BfmI CTRYAG 1 cut(s) 125
BfuAI ACCTGC 1 cut(s) 397
BisI GCNGC 4 cut(s) 25, 87, 152, 488
BlpI GCTNAGC 2 cut(s) 483, 491
BlsI GCNGC 4 cut(s) 26, 88, 153, 489
Bme1390I CCNGG 2 cut(s) 134, 229
Bme18I GGWCC 1 cut(s) 40
BmgBI CACGTC 1 cut(s) 173
BmgT120I GGNCC 3 cut(s) 22, 40, 761
BmiI GGNNCC 4 cut(s) 42, 76, 163, 304
BmrFI CCNGG 2 cut(s) 134, 229
BmsI GCATC 1 cut(s) 745
BoxI GACNNNNGTC 1 cut(s) 773
BplI GAGNNNNNCTC 2 cut(s) 722, 754
Bpu1102I GCTNAGC 2 cut(s) 483, 491
BpuEI CTTGAG 1 cut(s) 722
BpuMI CCSGG 2 cut(s) 134, 229
BsaBI GATNNNNATC 1 cut(s) 672
BsaJI CCNNGG 6 cut(s) 18, 33, 240, 291, 306, 591
BsaWI WCCGGW 1 cut(s) 65
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 158, 228, 312, 730
Bse118I RCCGGY 1 cut(s) 65
Bse3DI GCAATG 3 cut(s) 743, 924, 942
Bse8I GATNNNNATC 1 cut(s) 672
BseDI CCNNGG 6 cut(s) 18, 33, 240, 291, 306, 591
BseGI GGATG 2 cut(s) 102, 421
BseJI GATNNNNATC 1 cut(s) 672
BseLI CCNNNNNNNGG 5 cut(s) 85, 158, 228, 312, 730
BseMI GCAATG 3 cut(s) 743, 924, 942
BseMII CTCAG 3 cut(s) 474, 482, 725
BsePI GCGCGC 1 cut(s) 13
BseRI GAGGAG 1 cut(s) 308
BseX3I CGGCCG 1 cut(s) 288
BseXI GCAGC 1 cut(s) 499
Bsh1236I CGCG 4 cut(s) 15, 146, 260, 384
Bsh1285I CGRYCG 2 cut(s) 206, 291
BshFI GGCC 5 cut(s) 24, 89, 245, 290, 763
BshTI ACCGGT 1 cut(s) 65
BsiEI CGRYCG 2 cut(s) 206, 291
BsiHKAI GWGCWC 2 cut(s) 141, 708
BsiSI CCGG 4 cut(s) 66, 134, 165, 229
BslFI GGGAC 3 cut(s) 106, 446, 821
BslI CCNNNNNNNGG 5 cut(s) 85, 158, 228, 312, 730
BsmAI GTCTC 2 cut(s) 524, 761
BsmBI CGTCTC 1 cut(s) 524
BsmFI GGGAC 3 cut(s) 106, 446, 821
BsnI GGCC 5 cut(s) 24, 89, 245, 290, 763
Bsp1286I GDGCHC 3 cut(s) 141, 164, 708
Bsp1407I TGTACA 1 cut(s) 966
Bsp143I GATC 8 cut(s) 129, 203, 267, 302, 583, 673, 725, 828
Bsp1720I GCTNAGC 2 cut(s) 483, 491
Bsp19I CCATGG 1 cut(s) 591
Bsp68I TCGCGA 1 cut(s) 384
BspANI GGCC 5 cut(s) 24, 89, 245, 290, 763
BspCNI CTCAG 3 cut(s) 475, 483, 724
BspFNI CGCG 4 cut(s) 15, 146, 260, 384
BspLI GGNNCC 4 cut(s) 42, 76, 163, 304
BspMAI CTGCAG 1 cut(s) 129
BspMI ACCTGC 1 cut(s) 397
BspPI GGATC 4 cut(s) 124, 275, 297, 310
BsrBI CCGCTC 1 cut(s) 81
BsrDI GCAATG 3 cut(s) 743, 924, 942
BsrFI RCCGGY 1 cut(s) 65
BsrGI TGTACA 1 cut(s) 966
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 6 cut(s) 18, 33, 240, 291, 306, 591
BssHII GCGCGC 1 cut(s) 13
BssMI GATC 8 cut(s) 129, 203, 267, 302, 583, 673, 725, 828
BssT1I CCWWGG 2 cut(s) 18, 591
Bst4CI ACNGT 4 cut(s) 784, 845, 919, 966
BstAUI TGTACA 1 cut(s) 966
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 3 cut(s) 483, 491, 711
BstDSI CCRYGG 3 cut(s) 33, 306, 591
BstF5I GGATG 2 cut(s) 102, 421
BstFNI CGCG 4 cut(s) 15, 146, 260, 384
BstHHI GCGC 3 cut(s) 15, 17, 260
BstKTI GATC 8 cut(s) 132, 206, 270, 305, 586, 676, 728, 831
BstMAI GTCTC 2 cut(s) 524, 761
BstMBI GATC 8 cut(s) 129, 203, 267, 302, 583, 673, 725, 828
BstMCI CGRYCG 2 cut(s) 206, 291
BstMWI GCNNNNNNNGC 1 cut(s) 21
BstNSI RCATGY 1 cut(s) 658
BstPAI GACNNNNGTC 1 cut(s) 773
BstSCI CCNGG 2 cut(s) 132, 227
BstSFI CTRYAG 1 cut(s) 125
BstUI CGCG 4 cut(s) 15, 146, 260, 384
BstV1I GCAGC 1 cut(s) 499
BstX2I RGATCY 2 cut(s) 129, 302
BstYI RGATCY 2 cut(s) 129, 302
BstZI CGGCCG 1 cut(s) 288
BsuRI GGCC 5 cut(s) 24, 89, 245, 290, 763
BtgI CCRYGG 3 cut(s) 33, 306, 591
BtgZI GCGATG 1 cut(s) 681
BtrI CACGTC 1 cut(s) 173
BtsCI GGATG 2 cut(s) 102, 421
BtsIMutI CAGTG 1 cut(s) 780
BtuMI TCGCGA 1 cut(s) 384
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 1 cut(s) 15
CfoI GCGC 3 cut(s) 15, 17, 260
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 3 cut(s) 22, 40, 761
Csp6I GTAC 2 cut(s) 962, 967
CspAI ACCGGT 1 cut(s) 65
CviAII CATG 3 cut(s) 592, 655, 677
CviQI GTAC 2 cut(s) 962, 967
DdeI CTNAG 3 cut(s) 483, 491, 711
DpnI GATC 8 cut(s) 131, 205, 269, 304, 585, 675, 727, 830
DpnII GATC 8 cut(s) 129, 203, 267, 302, 583, 673, 725, 828
DraI TTTAAA 1 cut(s) 808
EaeI YGGCCR 3 cut(s) 87, 243, 288
EagI CGGCCG 1 cut(s) 288
EciI GGCGGA 2 cut(s) 53, 202
Ecl136II GAGCTC 1 cut(s) 139
EclXI CGGCCG 1 cut(s) 288
Eco130I CCWWGG 2 cut(s) 18, 591
Eco24I GRGCYC 2 cut(s) 141, 164
Eco32I GATATC 1 cut(s) 428
Eco47I GGWCC 1 cut(s) 40
Eco52I CGGCCG 1 cut(s) 288
Eco53kI GAGCTC 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 139
EcoRV GATATC 1 cut(s) 428
EcoT14I CCWWGG 2 cut(s) 18, 591
EcoT38I GRGCYC 2 cut(s) 141, 164
ErhI CCWWGG 2 cut(s) 18, 591
Esp3I CGTCTC 1 cut(s) 524
FaeI CATG 3 cut(s) 595, 658, 680
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 3 cut(s) 106, 446, 821
FatI CATG 3 cut(s) 591, 654, 676
FauI CCCGC 2 cut(s) 51, 86
FbaI TGATCA 1 cut(s) 673
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 4 cut(s) 25, 87, 152, 488
FokI GGATG 2 cut(s) 89, 428
FriOI GRGCYC 2 cut(s) 141, 164
Fsp4HI GCNGC 4 cut(s) 25, 87, 152, 488
FspBI CTAG 4 cut(s) 606, 773, 797, 900
GlaI GCGC 3 cut(s) 14, 16, 259
GluI GCNGC 4 cut(s) 25, 87, 152, 488
HaeIII GGCC 5 cut(s) 24, 89, 245, 290, 763
HapII CCGG 4 cut(s) 66, 134, 165, 229
HhaI GCGC 3 cut(s) 15, 17, 260
Hin1II CATG 3 cut(s) 595, 658, 680
Hin6I GCGC 3 cut(s) 13, 15, 258
HinP1I GCGC 3 cut(s) 13, 15, 258
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 5 cut(s) 218, 323, 360, 379, 769
HpaII CCGG 4 cut(s) 66, 134, 165, 229
HphI GGTGA 4 cut(s) 133, 167, 569, 683
Hpy166II GTNNAC 2 cut(s) 196, 969
Hpy188I TCNGA 6 cut(s) 203, 267, 353, 415, 725, 730
Hpy188III TCNNGA 4 cut(s) 333, 383, 431, 900
Hpy8I GTNNAC 2 cut(s) 196, 969
Hpy99I CGWCG 2 cut(s) 197, 344
HpyAV CCTTC 6 cut(s) 60, 165, 232, 448, 698, 704
HpyCH4III ACNGT 4 cut(s) 784, 845, 919, 966
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 7 cut(s) 127, 441, 461, 515, 743, 758, 947
HpyF10VI GCNNNNNNNGC 1 cut(s) 21
HpyF3I CTNAG 3 cut(s) 483, 491, 711
HpySE526I ACGT 1 cut(s) 172
Hsp92II CATG 3 cut(s) 595, 658, 680
HspAI GCGC 3 cut(s) 13, 15, 258
Ksp22I TGATCA 1 cut(s) 673
Kzo9I GATC 8 cut(s) 129, 203, 267, 302, 583, 673, 725, 828
LmnI GCTCC 4 cut(s) 80, 136, 167, 595
Lsp1109I GCAGC 1 cut(s) 499
LweI GCATC 1 cut(s) 745
MaeI CTAG 4 cut(s) 606, 773, 797, 900
MaeII ACGT 1 cut(s) 172
MaeIII GTNAC 3 cut(s) 173, 644, 778
MalI GATC 8 cut(s) 131, 205, 269, 304, 585, 675, 727, 830
MbiI CCGCTC 1 cut(s) 81
MboI GATC 8 cut(s) 129, 203, 267, 302, 583, 673, 725, 828
MfeI CAATTG 1 cut(s) 477
MflI RGATCY 2 cut(s) 129, 302
MhlI GDGCHC 3 cut(s) 141, 164, 708
MluCI AATT 6 cut(s) 107, 477, 524, 613, 895, 906
MlyI GAGTC 3 cut(s) 227, 332, 763
MmeI TCCRAC 1 cut(s) 766
MseI TTAA 6 cut(s) 29, 393, 504, 566, 717, 807
MslI CAYNNNNRTG 2 cut(s) 653, 657
MspA1I CMGCKG 1 cut(s) 490
MspI CCGG 4 cut(s) 66, 134, 165, 229
MspR9I CCNGG 2 cut(s) 134, 229
MunI CAATTG 1 cut(s) 477
MvnI CGCG 4 cut(s) 15, 146, 260, 384
MwoI GCNNNNNNNGC 1 cut(s) 21
NciI CCSGG 2 cut(s) 134, 229
NcoI CCATGG 1 cut(s) 591
NdeII GATC 8 cut(s) 129, 203, 267, 302, 583, 673, 725, 828
NlaIII CATG 3 cut(s) 595, 658, 680
NlaIV GGNNCC 4 cut(s) 42, 76, 163, 304
NmeAIII GCCGAG 4 cut(s) 221, 241, 316, 346
NmuCI GTSAC 3 cut(s) 173, 644, 778
NruI TCGCGA 1 cut(s) 384
NspI RCATGY 1 cut(s) 658
OliI CACNNNNGTG 1 cut(s) 657
PauI GCGCGC 1 cut(s) 13
PfeI GAWTC 2 cut(s) 360, 379
PfoI TCCNGGA 1 cut(s) 132
PinAI ACCGGT 1 cut(s) 65
PkrI GCNGC 4 cut(s) 26, 88, 153, 489
Ple19I CGATCG 1 cut(s) 206
PleI GAGTC 3 cut(s) 226, 331, 763
PpsI GAGTC 3 cut(s) 226, 331, 763
PshAI GACNNNNGTC 1 cut(s) 773
Psp124BI GAGCTC 1 cut(s) 141
PspN4I GGNNCC 4 cut(s) 42, 76, 163, 304
PspPI GGNCC 3 cut(s) 22, 40, 761
PstI CTGCAG 1 cut(s) 129
PsuI RGATCY 2 cut(s) 129, 302
PteI GCGCGC 1 cut(s) 13
PvuI CGATCG 1 cut(s) 206
PvuII CAGCTG 1 cut(s) 490
RruI TCGCGA 1 cut(s) 384
RsaI GTAC 2 cut(s) 963, 968
RsaNI GTAC 2 cut(s) 962, 967
RseI CAYNNNNRTG 2 cut(s) 653, 657
SacI GAGCTC 1 cut(s) 141
SalI GTCGAC 1 cut(s) 194
SaqAI TTAA 6 cut(s) 29, 393, 504, 566, 717, 807
SatI GCNGC 4 cut(s) 25, 87, 152, 488
Sau3AI GATC 8 cut(s) 129, 203, 267, 302, 583, 673, 725, 828
Sau96I GGNCC 3 cut(s) 22, 40, 761
SchI GAGTC 3 cut(s) 227, 332, 763
ScrFI CCNGG 2 cut(s) 134, 229
SduI GDGCHC 3 cut(s) 141, 164, 708
SetI ASST 6 cut(s) 141, 175, 411, 459, 492, 696
SfaNI GCATC 1 cut(s) 745
SfcI CTRYAG 1 cut(s) 125
SinI GGWCC 1 cut(s) 40
SmiI ATTTAAAT 1 cut(s) 808
SmiMI CAYNNNNRTG 2 cut(s) 653, 657
SmlI CTYRAG 1 cut(s) 701
SmoI CTYRAG 1 cut(s) 701
Sse9I AATT 6 cut(s) 107, 477, 524, 613, 895, 906
SspMI CTAG 4 cut(s) 606, 773, 797, 900
SstI GAGCTC 1 cut(s) 141
StyD4I CCNGG 2 cut(s) 132, 227
StyI CCWWGG 2 cut(s) 18, 591
SwaI ATTTAAAT 1 cut(s) 808
TaaI ACNGT 4 cut(s) 784, 845, 919, 966
TaiI ACGT 1 cut(s) 175
TaqI TCGA 2 cut(s) 114, 195
TasI AATT 6 cut(s) 107, 477, 524, 613, 895, 906
TatI WGTACW 2 cut(s) 961, 966
TauI GCSGC 3 cut(s) 27, 89, 154
TfiI GAWTC 2 cut(s) 360, 379
Tru1I TTAA 6 cut(s) 29, 393, 504, 566, 717, 807
Tru9I TTAA 6 cut(s) 29, 393, 504, 566, 717, 807
TscAI CASTG 1 cut(s) 787
TseFI GTSAC 3 cut(s) 173, 644, 778
TseI GCWGC 1 cut(s) 487
Tsp45I GTSAC 3 cut(s) 173, 644, 778
TspDTI ATGAA 3 cut(s) 91, 352, 996
TspGWI ACGGA 2 cut(s) 295, 357
TspRI CASTG 1 cut(s) 787
VpaK11BI GGWCC 1 cut(s) 40
XapI RAATTY 3 cut(s) 613, 895, 906
XbaI TCTAGA 1 cut(s) 899
XceI RCATGY 1 cut(s) 658
XcmI CCANNNNNNNNNTGG 1 cut(s) 503
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 4 cut(s) 606, 773, 797, 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.