MD12G1187600.v1.1

Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005488
Physical Location & Seq
Forward (+)
26975674 .. 26976090
417 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1187600.v1.1.491

Sequence Viewer

Length: 417 bp
ATGGCTAGCTCTGGACAGCTCCTTAAGCTTGCATGCTTGGTGGTGGTGATGTTCTGCATGGTGGCTTGTGTCCCCAAGGCCGAGGCAGCTGTGACATGCGGTCAGATGGTGAGTGACCTGACTCCATGCATTTCCTACGTGAAGACCGGCGGGGCATTGGCCCCCGCTTGTTGCAACGGGGTCAGGACCCTCTTCACCCTGGCTCAAACCAACCCTGACCGCCAAAGTGTCTGCAACTGCTTGAAGCAAGCAATCAATGGCATCCCTTACACCAATACCAACGCTGGCCTTGCTGCTGGCCTTCCTGCCAAGTGTAGTGTCAACATTCCGTACAAGATCAGCCCCTCTACAGACTGCAAAAGGTCTTATAAATTAATTAAACTCATTTTAACAACAATTTTAGTTTTCAGTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0006810 GO:0006869 GO:0008150 GO:0010876 GO:0016020 GO:0033036 GO:0051179 GO:0051234 GO:0071702
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2 Tryp_alpha_amyl

Protein Analysis

139

Amino Acids

14.48

Weight (kDa)

9.04

Isoelectric Point (pI)

40.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 33 - 119 2.2e-14 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 369
AciI CCGC 4 cut(s) 99, 150, 165, 220
AfaI GTAC 1 cut(s) 332
AflII CTTAAG 1 cut(s) 23
AgsI TTSAA 1 cut(s) 244
AhdI GACNNNNNGTC 1 cut(s) 99
AjnI CCWGG 1 cut(s) 198
AluBI AGCT 4 cut(s) 9, 19, 28, 89
AluI AGCT 4 cut(s) 9, 19, 28, 89
AoxI GGCC 4 cut(s) 78, 159, 286, 298
ApeKI GCWGC 2 cut(s) 86, 293
AseI ATTAAT 1 cut(s) 374
AspS9I GGNCC 2 cut(s) 160, 186
AsuHPI GGTGA 3 cut(s) 58, 121, 187
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 1 cut(s) 186
BbsI GAAGAC 1 cut(s) 149
BbvI GCAGC 2 cut(s) 98, 280
BccI CCATC 1 cut(s) 100
BciT130I CCWGG 1 cut(s) 200
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 348
BfrI CTTAAG 1 cut(s) 23
BisI GCNGC 2 cut(s) 87, 294
BlsI GCNGC 2 cut(s) 88, 295
Bme1390I CCNGG 1 cut(s) 200
Bme18I GGWCC 1 cut(s) 186
BmeRI GACNNNNNGTC 1 cut(s) 99
BmgT120I GGNCC 2 cut(s) 160, 186
BmiI GGNNCC 2 cut(s) 162, 188
BmrFI CCNGG 1 cut(s) 200
BmsI GCATC 1 cut(s) 270
BmtI GCTAGC 1 cut(s) 9
BpiI GAAGAC 1 cut(s) 149
BsaAI YACGTR 1 cut(s) 139
BsaJI CCNNGG 3 cut(s) 75, 81, 198
Bse118I RCCGGY 1 cut(s) 146
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 3 cut(s) 75, 81, 198
BseGI GGATG 1 cut(s) 261
BseXI GCAGC 2 cut(s) 98, 280
BshFI GGCC 4 cut(s) 80, 161, 288, 300
BsiSI CCGG 1 cut(s) 147
BslFI GGGAC 1 cut(s) 56
BsmFI GGGAC 1 cut(s) 56
BsnI GGCC 4 cut(s) 80, 161, 288, 300
Bsp143I GATC 1 cut(s) 336
BspACI CCGC 4 cut(s) 99, 150, 165, 220
BspANI GGCC 4 cut(s) 80, 161, 288, 300
BspLI GGNNCC 2 cut(s) 162, 188
BspOI GCTAGC 1 cut(s) 9
BspTI CTTAAG 1 cut(s) 23
BsrFI RCCGGY 1 cut(s) 146
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 3 cut(s) 75, 81, 198
BssMI GATC 1 cut(s) 336
BssT1I CCWWGG 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 200
Bst6I CTCTTC 1 cut(s) 197
BstAFI CTTAAG 1 cut(s) 23
BstBAI YACGTR 1 cut(s) 139
BstC8I GCNNGC 6 cut(s) 7, 30, 34, 249, 286, 298
BstF5I GGATG 1 cut(s) 261
BstKTI GATC 1 cut(s) 339
BstMBI GATC 1 cut(s) 336
BstMWI GCNNNNNNNGC 3 cut(s) 25, 86, 290
BstNI CCWGG 1 cut(s) 200
BstNSI RCATGY 2 cut(s) 36, 99
BstSCI CCNGG 1 cut(s) 198
BstSFI CTRYAG 1 cut(s) 348
BstV1I GCAGC 2 cut(s) 98, 280
BstV2I GAAGAC 1 cut(s) 149
BsuRI GGCC 4 cut(s) 80, 161, 288, 300
BtsCI GGATG 1 cut(s) 261
BtsIMutI CAGTG 1 cut(s) 415
Cac8I GCNNGC 6 cut(s) 7, 30, 34, 249, 286, 298
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 2 cut(s) 160, 186
Csp6I GTAC 1 cut(s) 331
CviAII CATG 4 cut(s) 33, 58, 96, 126
CviQI GTAC 1 cut(s) 331
DpnI GATC 1 cut(s) 338
DpnII GATC 1 cut(s) 336
DriI GACNNNNNGTC 1 cut(s) 99
Eam1104I CTCTTC 1 cut(s) 197
Eam1105I GACNNNNNGTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 186
EcoO109I RGGNCCY 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 1 cut(s) 75
EcoT22I ATGCAT 1 cut(s) 131
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 4 cut(s) 36, 61, 99, 129
FaiI YATR 5 cut(s) 34, 59, 97, 127, 369
FaqI GGGAC 1 cut(s) 56
FatI CATG 4 cut(s) 32, 57, 95, 125
FauI CCCGC 2 cut(s) 143, 172
Fnu4HI GCNGC 2 cut(s) 87, 294
FokI GGATG 1 cut(s) 248
Fsp4HI GCNGC 2 cut(s) 87, 294
FspBI CTAG 1 cut(s) 6
GluI GCNGC 2 cut(s) 87, 294
HaeIII GGCC 4 cut(s) 80, 161, 288, 300
HapII CCGG 1 cut(s) 147
Hin1II CATG 4 cut(s) 36, 61, 99, 129
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 1 cut(s) 121
HpaII CCGG 1 cut(s) 147
HphI GGTGA 3 cut(s) 58, 121, 187
Hpy166II GTNNAC 1 cut(s) 322
Hpy188I TCNGA 1 cut(s) 105
Hpy188III TCNNGA 2 cut(s) 12, 184
Hpy8I GTNNAC 1 cut(s) 322
HpyAV CCTTC 1 cut(s) 311
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 6 cut(s) 32, 57, 129, 174, 234, 357
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 86, 290
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 4 cut(s) 36, 61, 99, 129
Kzo9I GATC 1 cut(s) 336
LmnI GCTCC 1 cut(s) 24
LpnPI CCDG 9 cut(s) 131, 160, 169, 185, 212, 228, 270, 282, 318
Lsp1109I GCAGC 2 cut(s) 98, 280
LweI GCATC 1 cut(s) 270
MaeI CTAG 1 cut(s) 6
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 2 cut(s) 91, 113
MalI GATC 1 cut(s) 338
MboI GATC 1 cut(s) 336
MboII GAAGA 2 cut(s) 154, 184
MluCI AATT 3 cut(s) 371, 375, 396
MlyI GAGTC 1 cut(s) 115
MnlI CCTC 3 cut(s) 76, 200, 355
Mph1103I ATGCAT 1 cut(s) 131
MseI TTAA 4 cut(s) 24, 374, 378, 389
MspA1I CMGCKG 1 cut(s) 89
MspCI CTTAAG 1 cut(s) 23
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 1 cut(s) 200
MvaI CCWGG 1 cut(s) 200
MwoI GCNNNNNNNGC 3 cut(s) 25, 86, 290
NdeII GATC 1 cut(s) 336
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 4 cut(s) 36, 61, 99, 129
NlaIV GGNNCC 2 cut(s) 162, 188
NmeAIII GCCGAG 1 cut(s) 106
NmuCI GTSAC 2 cut(s) 91, 113
NsiI ATGCAT 1 cut(s) 131
NspI RCATGY 2 cut(s) 36, 99
PacI TTAATTAA 1 cut(s) 378
PaeI GCATGC 1 cut(s) 36
PkrI GCNGC 2 cut(s) 88, 295
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
Ppu21I YACGTR 1 cut(s) 139
PpuMI RGGWCCY 1 cut(s) 186
PshBI ATTAAT 1 cut(s) 374
PsiI TTATAA 1 cut(s) 369
Psp5II RGGWCCY 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PspN4I GGNNCC 2 cut(s) 162, 188
PspPI GGNCC 2 cut(s) 160, 186
PspPPI RGGWCCY 1 cut(s) 186
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
SaqAI TTAA 4 cut(s) 24, 374, 378, 389
SatI GCNGC 2 cut(s) 87, 294
Sau3AI GATC 1 cut(s) 336
Sau96I GGNCC 2 cut(s) 160, 186
SchI GAGTC 1 cut(s) 115
ScrFI CCNGG 1 cut(s) 200
SetI ASST 7 cut(s) 11, 21, 30, 91, 120, 141, 365
SfaNI GCATC 1 cut(s) 270
SfcI CTRYAG 1 cut(s) 348
SinI GGWCC 1 cut(s) 186
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
SphI GCATGC 1 cut(s) 36
Sse9I AATT 3 cut(s) 371, 375, 396
SsiI CCGC 4 cut(s) 99, 150, 165, 220
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 1 cut(s) 75
TaiI ACGT 1 cut(s) 141
TasI AATT 3 cut(s) 371, 375, 396
Tru1I TTAA 4 cut(s) 24, 374, 378, 389
Tru9I TTAA 4 cut(s) 24, 374, 378, 389
TscAI CASTG 1 cut(s) 415
TseFI GTSAC 2 cut(s) 91, 113
TseI GCWGC 2 cut(s) 86, 293
Tsp45I GTSAC 2 cut(s) 91, 113
TspGWI ACGGA 1 cut(s) 318
TspRI CASTG 1 cut(s) 415
Vha464I CTTAAG 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 186
VspI ATTAAT 1 cut(s) 374
XceI RCATGY 2 cut(s) 36, 99
XspI CTAG 1 cut(s) 6
Zsp2I ATGCAT 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.