Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0004290

Physical Location & Seq

Genomic Coordinates Reverse (-)

27052640 .. 27057911

Gene Structure 5272 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1188800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1881 bp

ATGAAGGAAAAGCTTGGATTTTTAGTGTGTGTGTGGTTTCTGCTATGTAGGGTTTGTGTGGGGAGGTTCGTGGTGGAGAAGAACAGGTTGAAAGTGACTTCTCCGAGCTCGATGAAGGGCGATTACGAATGTGCGATTGGGAACTTTGGGGTTCCTCCATACGGAGGAACGTTGGTCGGAACTGTGTACTATCCGAAGACCAATCAGATGGCATGCAAAGGGTTTGAAGATACCGACGAAACCTTCAAACCGAAGCCCCGTGGTCTGCCGACGTTTGTTCTTGTCGATCGAGGAGATTGTTACTTCACAACGAAGGCATGGAATGCACAGAAAGCGGGAGCGGCCGCCATTCTTGTTGCAGATGACAGGAATGAACCGTTGATTACCATGGACACTCCTGAAGAAGAAAATGCGGATGCTGCTGATTATCTGCAGAATATCACAATTCCTTCGGCTCTCATTAGCAAAGAGTTGGGAGATAGTATAAAAAAATCTCTATCGGGTGATGAGATGGTCAACATAAATCTAGACTGGACAGAGGCTCTTCCACATCCAGATGAACGTGTTGAGTACGAGTTTTGGACGAATAGCAATGATGTGTGCGGGCCAAAGTGCGACAGCCAGATTGAGTTTGTGAAGAACTTTAAAGGAGCAGCTCAGGTACTTGAGCGGAAGGGGTACACCCAATTTACTCCACACTATATAACATGGTACTGTCCAGAAGCTTTTGTTTCGAGCAAACAGTGCGAGTCTCAGTGTATCAATCATGGGAGGTACTGTGCTCCTGATCCAGAGCAAGATTTCAGTGAAGGTTATGATGGGAAAGATGTTGTGGTCCAAAATCTCCGTCAAGCTTGCTTCTATAAAGTGGCCAATGAAAGTGGAAAGCCATGGCTTTGGTGGGACTATGTCACTGACTTTGCGATACGTTGCCCAATGAAAGAGAAGAAGTACAACCAAGAATGTGCAAATCAAGTTATTGAATCCCTTGGTGCTGATCTGAAGAAGATACAGGAATGTATTGGCGACCCTGAAGCTGATGAGGAGAATGCAGTTCTGAAGGCCGAACAGGATGCCCAGATTGGCAAGGGCTCCCGTGGAGATGTTACAATATTGCCAACTCTTGTTATAAACCGCGTACAGTACAGAGGGAAACTGGACAAAGGAGCTGTTCTCAAAGCCATCTGTTCTGGTTTCCTAGAGAACACTGAGCCCGCTGTCTGTTTAAGTGAAGATGTGCAAACAAATGAGTGTTTGGAAAACAATGGAGGTTGCTGGTATGACAGAGCTGCTAACCTTACGGCTTGCAGGGATACGTTCCGCGGACGAGTGTGTGAATGCCCTATTGTGCAAGGCGTAAAGTTTGTTGGCGATGGGTATACTCACTGTGAAGCTACAGGAGCATTACGTTGTGGAATCAACAATGGAGGTTGTTGGAAGGAAACCCGAACCGGCAGTACTTACTCCGCTTGTCGTGATGACCTTACAAACGGTTGCAGTTGTCCACCAGGATTCAAGGGTGATGGAGAGAAAACATGTGAAGATGTGGATGAGTGCAAGGAGAAAACGGCTTGCCAATGTGCACACTGCAAATGCAAGAATACTTGGGGCAGTTACGACTGCAGTTGCGGTGGTGGTTTACTGTACATGCGAGGATATGATGCATGCATAGGTAAAAACGCCAGTGGTTCTGGGTCTAGCCGGGGATTAATATGGATAATCATTCTCTGCTTGGGTGCTGTCGGAGTTGGGGGATACATAGTTTACAAGTATCGAATCAGGAGATACATGGATTCAGAGATCCGGGCAATCATGGCGCAGTACATGCCGTTGGATAATGAAGGAGCAGTTCCCGGCCACGTCTCCCGCGGGGATATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0005048 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005783 GO:0005794 GO:0005802 GO:0005886 GO:0006605 GO:0006623 GO:0006810 GO:0006886 GO:0006892 GO:0006896 GO:0007034 GO:0008104 GO:0008150 GO:0009940 GO:0010008 GO:0010209 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030135 GO:0030136 GO:0030659 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031902 GO:0031982 GO:0031984 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0071944 GO:0072594 GO:0072665 GO:0072666 GO:0097708 GO:0098588 GO:0098791 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA cEGF

Protein Analysis

627

Amino Acids

69.24

Weight (kDa)

5.13

Isoelectric Point (pI)

34.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	61 - 162	2.6e-15	PA domain
VSR_TRX	PF25011	189 - 396	2.5e-105	Vacuolar sorting receptor thioredoxin-like domain
cEGF	PF12662	499 - 518	4e-07	Complement Clr-like EGF-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004290)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30290 AT2G30290
fragaria_vesca	FvH4_6g09810 FvH4_6g09810 FvH4_6g09810
malus_domestica	MD04G1175100.v1.1 MD12G1188800.v1.1
prunus_persica	Prupe.6G294400_v2.0.a1 Prupe.6G294400_v2.0.a1
pyrus_communis	pycom04g15450 pycom12g17630
rosa_chinensis	RchiOBHm_Chr3g0460421 RchiOBHm_Chr6g0252231
rosa_laevigata	RLG00000025013
rosa_multiflora	Rmu_co8220956.1_g000001 Rmu_co8306627.1_g000001 Rmu_sc0004267.1_g000012 Rmu_sc0006252.1_g000025 Rmu_sc0009799.1_g000013 Rmu_sc0021028.1_g000001 Rmu_sc0025932.1_g000001
rosa_roxburghii	Rroxscaffold_6G00419500 Rroxscaffold_7G00216220
rosa_rugosa	Rorug03G0042500
rosa_samantha	Rh3AG100500 Rh3BG104500 Rh3CG106300 Rh3DG105800 Rh6AG020100 Rh6BG015600
rosa_wichuraiana	Rw3G008550 Rw6G001650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1130
AccBSI	CCGCTC	2 cut(s)	341, 670
AccI	GTMKAC	1 cut(s)	1379
AccII	CGCG	3 cut(s)	1137, 1323, 1869
AclI	AACGTT	1 cut(s)	170
AclWI	GGATC	2 cut(s)	782, 1795
AcoI	YGGCCR	3 cut(s)	342, 870, 1855
AcuI	CTGAAG	4 cut(s)	420, 1022, 1053, 1079
AfiI	CCNNNNNNNGG	2 cut(s)	161, 164
AflIII	ACRYGT	2 cut(s)	562, 1535
AgsI	TTSAA	5 cut(s)	91, 227, 247, 983, 1516
AjiI	CACGTC	1 cut(s)	1861
AjnI	CCWGG	1 cut(s)	1507
AjuI	GAANNNNNNNTTGG	2 cut(s)	120, 152
AluBI	AGCT	9 cut(s)	13, 108, 656, 725, 854, 1037, 1169, 1289, 1394
AluI	AGCT	9 cut(s)	13, 108, 656, 725, 854, 1037, 1169, 1289, 1394
Alw21I	GWGCWC	3 cut(s)	110, 784, 1585
Alw26I	GTCTC	2 cut(s)	756, 1867
Alw44I	GTGCAC	1 cut(s)	1581
AlwI	GGATC	2 cut(s)	782, 1795
AoxI	GGCC	5 cut(s)	342, 605, 870, 1062, 1855
ApaLI	GTGCAC	1 cut(s)	1581
ApeKI	GCWGC	3 cut(s)	419, 653, 1289
ArsI	GACNNNNNNTTYG	2 cut(s)	832, 864
AseI	ATTAAT	1 cut(s)	1709
AspLEI	GCGC	1 cut(s)	1819
AspS9I	GGNCC	2 cut(s)	605, 835
AsuC2I	CCSGG	3 cut(s)	1703, 1805, 1854
AsuHPI	GGTGA	2 cut(s)	515, 1532
AvaII	GGWCC	1 cut(s)	835
BaeGI	GKGCMC	1 cut(s)	1585
BalI	TGGCCA	1 cut(s)	872
BanII	GRGCYC	3 cut(s)	110, 1094, 1215
BarI	GAAGNNNNNNTAC	2 cut(s)	107, 139
BbsI	GAAGAC	1 cut(s)	203
Bbv12I	GWGCWC	3 cut(s)	110, 784, 1585
BbvI	GCAGC	3 cut(s)	406, 665, 1276
BccI	CCATC	6 cut(s)	202, 505, 812, 1190, 1367, 1517
BceAI	ACGGC	3 cut(s)	1317, 1584, 1813
BcgI	CGANNNNNNTGC	2 cut(s)	1055, 1089
BciT130I	CCWGG	1 cut(s)	1509
BciVI	GTATCC	2 cut(s)	1306, 1748
BcnI	CCSGG	3 cut(s)	1703, 1805, 1854
BcoDI	GTCTC	2 cut(s)	756, 1867
BfaI	CTAG	3 cut(s)	527, 1199, 1698
BfmI	CTRYAG	3 cut(s)	431, 1395, 1621
BfuI	GTATCC	2 cut(s)	1306, 1748
BisI	GCNGC	5 cut(s)	342, 345, 420, 654, 1290
BlsI	GCNGC	5 cut(s)	343, 346, 421, 655, 1291
BmcAI	AGTACT	1 cut(s)	1459
Bme1390I	CCNGG	4 cut(s)	1509, 1703, 1805, 1854
Bme18I	GGWCC	1 cut(s)	835
BmgBI	CACGTC	1 cut(s)	1861
BmgT120I	GGNCC	2 cut(s)	605, 835
BmiI	GGNNCC	2 cut(s)	153, 1093
BmrFI	CCNGG	4 cut(s)	1509, 1703, 1805, 1854
BmsI	GCATC	3 cut(s)	406, 1063, 1651
BpiI	GAAGAC	1 cut(s)	203
BplI	GAGNNNNNCTC	2 cut(s)	1158, 1190
Bpu10I	CCTNAGC	1 cut(s)	657
BpuEI	CTTGAG	1 cut(s)	686
BpuMI	CCSGG	3 cut(s)	1703, 1805, 1854
BsaJI	CCNNGG	8 cut(s)	259, 387, 890, 988, 1096, 1321, 1702, 1867
BsaXI	ACNNNNNCTCC	2 cut(s)	763, 793
Bsc4I	CCNNNNNNNGG	2 cut(s)	161, 164
Bse118I	RCCGGY	1 cut(s)	1451
Bse1I	ACTGG	3 cut(s)	536, 1161, 1683
Bse3DI	GCAATG	1 cut(s)	598
BseBI	CCWGG	1 cut(s)	1509
BseDI	CCNNGG	8 cut(s)	259, 387, 890, 988, 1096, 1321, 1702, 1867
BseGI	GGATG	4 cut(s)	421, 550, 1078, 1555
BseLI	CCNNNNNNNGG	2 cut(s)	161, 164
BseMI	GCAATG	1 cut(s)	598
BseMII	CTCAG	3 cut(s)	671, 767, 1200
BseNI	ACTGG	3 cut(s)	536, 1161, 1683
BseRI	GAGGAG	2 cut(s)	306, 1058
BseSI	GKGCMC	1 cut(s)	1585
BseX3I	CGGCCG	1 cut(s)	342
BseXI	GCAGC	3 cut(s)	406, 665, 1276
Bsh1236I	CGCG	3 cut(s)	1137, 1323, 1869
Bsh1285I	CGRYCG	2 cut(s)	289, 345
BshFI	GGCC	5 cut(s)	344, 607, 872, 1064, 1857
BsiEI	CGRYCG	2 cut(s)	289, 345
BsiHKAI	GWGCWC	3 cut(s)	110, 784, 1585
BsiSI	CCGG	4 cut(s)	1452, 1702, 1804, 1854
BslFI	GGGAC	1 cut(s)	917
BslI	CCNNNNNNNGG	2 cut(s)	161, 164
BsmAI	GTCTC	2 cut(s)	756, 1867
BsmBI	CGTCTC	1 cut(s)	1867
BsmFI	GGGAC	1 cut(s)	917
BsmI	GAATGC	3 cut(s)	328, 1054, 1343
BsnI	GGCC	5 cut(s)	344, 607, 872, 1064, 1857
Bsp1286I	GDGCHC	5 cut(s)	110, 784, 1094, 1215, 1585
Bsp1407I	TGTACA	1 cut(s)	1644
Bsp143I	GATC	4 cut(s)	286, 787, 997, 1800
Bsp19I	CCATGG	2 cut(s)	387, 890
BspANI	GGCC	5 cut(s)	344, 607, 872, 1064, 1857
BspCNI	CTCAG	3 cut(s)	670, 766, 1201
BspFNI	CGCG	3 cut(s)	1137, 1323, 1869
BspLI	GGNNCC	2 cut(s)	153, 1093
BspMAI	CTGCAG	2 cut(s)	435, 1625
BspPI	GGATC	2 cut(s)	782, 1795
BspQI	GCTCTTC	1 cut(s)	549
BsrBI	CCGCTC	2 cut(s)	341, 670
BsrDI	GCAATG	1 cut(s)	598
BsrFI	RCCGGY	1 cut(s)	1451
BsrGI	TGTACA	1 cut(s)	1644
BsrI	ACTGG	3 cut(s)	536, 1161, 1683
BssAI	RCCGGY	1 cut(s)	1451
BssECI	CCNNGG	8 cut(s)	259, 387, 890, 988, 1096, 1321, 1702, 1867
BssMI	GATC	4 cut(s)	286, 787, 997, 1800
BssNAI	GTATAC	1 cut(s)	1380
BssT1I	CCWWGG	3 cut(s)	387, 890, 988
Bst1107I	GTATAC	1 cut(s)	1380
Bst2UI	CCWGG	1 cut(s)	1509
Bst4CI	ACNGT	9 cut(s)	184, 378, 716, 744, 779, 1143, 1388, 1493, 1644
Bst6I	CTCTTC	1 cut(s)	549
BstAPI	GCANNNNNTGC	3 cut(s)	323, 744, 1825
BstAUI	TGTACA	1 cut(s)	1644
BstC8I	GCNNGC	7 cut(s)	214, 605, 856, 1215, 1306, 1573, 1666
BstDEI	CTNAG	3 cut(s)	657, 753, 1209
BstDSI	CCRYGG	6 cut(s)	259, 387, 890, 1096, 1321, 1867
BstF5I	GGATG	4 cut(s)	421, 550, 1078, 1555
BstFNI	CGCG	3 cut(s)	1137, 1323, 1869
BstHHI	GCGC	1 cut(s)	1819
BstKTI	GATC	4 cut(s)	289, 790, 1000, 1803
BstMAI	GTCTC	2 cut(s)	756, 1867
BstMBI	GATC	4 cut(s)	286, 787, 997, 1800
BstMCI	CGRYCG	2 cut(s)	289, 345
BstMWI	GCNNNNNNNGC	8 cut(s)	323, 332, 341, 419, 744, 1400, 1814, 1825
BstNI	CCWGG	1 cut(s)	1509
BstNSI	RCATGY	5 cut(s)	216, 1539, 1651, 1668, 1828
BstSCI	CCNGG	4 cut(s)	1507, 1701, 1803, 1852
BstSFI	CTRYAG	3 cut(s)	431, 1395, 1621
BstSLI	GKGCMC	1 cut(s)	1585
BstUI	CGCG	3 cut(s)	1137, 1323, 1869
BstV1I	GCAGC	3 cut(s)	406, 665, 1276
BstV2I	GAAGAC	1 cut(s)	203
BstX2I	RGATCY	1 cut(s)	1800
BstXI	CCANNNNNNTGG	2 cut(s)	208, 897
BstYI	RGATCY	1 cut(s)	1800
BstZ17I	GTATAC	1 cut(s)	1380
BstZI	CGGCCG	1 cut(s)	342
BsuI	GTATCC	2 cut(s)	1306, 1748
BsuRI	GGCC	5 cut(s)	344, 607, 872, 1064, 1857
BtgI	CCRYGG	6 cut(s)	259, 387, 890, 1096, 1321, 1867
BtgZI	GCGATG	1 cut(s)	1386
BtrI	CACGTC	1 cut(s)	1861
BtsCI	GGATG	4 cut(s)	421, 550, 1078, 1555
BtsI	GCAGTG	1 cut(s)	1585
BtsIMutI	CAGTG	8 cut(s)	749, 761, 811, 912, 1206, 1384, 1585, 1690
Cac8I	GCNNGC	7 cut(s)	214, 605, 856, 1215, 1306, 1573, 1666
CciNI	GCGGCCGC	1 cut(s)	342
CfoI	GCGC	1 cut(s)	1819
Cfr10I	RCCGGY	1 cut(s)	1451
Cfr13I	GGNCC	2 cut(s)	605, 835
Cfr42I	CCGCGG	2 cut(s)	1324, 1870
CspCI	CAANNNNNGTGG	2 cut(s)	862, 897
DdeI	CTNAG	3 cut(s)	657, 753, 1209
DpnI	GATC	4 cut(s)	288, 789, 999, 1802
DpnII	GATC	4 cut(s)	286, 787, 997, 1800
DraI	TTTAAA	1 cut(s)	646
EaeI	YGGCCR	3 cut(s)	342, 870, 1855
EagI	CGGCCG	1 cut(s)	342
Eam1104I	CTCTTC	1 cut(s)	549
EarI	CTCTTC	1 cut(s)	549
Ecl136II	GAGCTC	1 cut(s)	108
EclXI	CGGCCG	1 cut(s)	342
Eco130I	CCWWGG	3 cut(s)	387, 890, 988
Eco24I	GRGCYC	3 cut(s)	110, 1094, 1215
Eco32I	GATATC	1 cut(s)	1876
Eco47I	GGWCC	1 cut(s)	835
Eco52I	CGGCCG	1 cut(s)	342
Eco53kI	GAGCTC	1 cut(s)	108
Eco57I	CTGAAG	4 cut(s)	420, 1022, 1053, 1079
EcoICRI	GAGCTC	1 cut(s)	108
EcoRII	CCWGG	1 cut(s)	1507
EcoRV	GATATC	1 cut(s)	1876
EcoT14I	CCWWGG	3 cut(s)	387, 890, 988
EcoT22I	ATGCAT	2 cut(s)	1666, 1670
EcoT38I	GRGCYC	3 cut(s)	110, 1094, 1215
ErhI	CCWWGG	3 cut(s)	387, 890, 988
Esp3I	CGTCTC	1 cut(s)	1867
FaqI	GGGAC	1 cut(s)	917
FauI	CCCGC	5 cut(s)	328, 596, 1222, 1862, 1874
FblI	GTMKAC	1 cut(s)	1379
Fnu4HI	GCNGC	5 cut(s)	342, 345, 420, 654, 1290
FokI	GGATG	4 cut(s)	428, 537, 1085, 1562
FriOI	GRGCYC	3 cut(s)	110, 1094, 1215
Fsp4HI	GCNGC	5 cut(s)	342, 345, 420, 654, 1290
FspBI	CTAG	3 cut(s)	527, 1199, 1698
GlaI	GCGC	1 cut(s)	1818
GluI	GCNGC	5 cut(s)	342, 345, 420, 654, 1290
HaeIII	GGCC	5 cut(s)	344, 607, 872, 1064, 1857
HapII	CCGG	4 cut(s)	1452, 1702, 1804, 1854
HhaI	GCGC	1 cut(s)	1819
Hin6I	GCGC	1 cut(s)	1817
HinP1I	GCGC	1 cut(s)	1817
HincII	GTYRAC	1 cut(s)	517
HindII	GTYRAC	1 cut(s)	517
HindIII	AAGCTT	3 cut(s)	11, 723, 852
HinfI	GANTC	6 cut(s)	749, 983, 1416, 1512, 1776, 1793
HpaII	CCGG	4 cut(s)	1452, 1702, 1804, 1854
HphI	GGTGA	2 cut(s)	515, 1532
Hpy166II	GTNNAC	8 cut(s)	187, 517, 681, 1380, 1505, 1583, 1640, 1765
Hpy188I	TCNGA	9 cut(s)	105, 179, 195, 207, 1002, 1059, 1745, 1798, 1880
Hpy188III	TCNNGA	8 cut(s)	398, 527, 554, 719, 785, 791, 1475, 1780
Hpy8I	GTNNAC	8 cut(s)	187, 517, 681, 1380, 1505, 1583, 1640, 1765
Hpy99I	CGWCG	2 cut(s)	239, 274
HpyAV	CCTTC	9 cut(s)	109, 253, 307, 459, 667, 803, 1054, 1432, 1835
HpyCH4III	ACNGT	9 cut(s)	184, 378, 716, 744, 779, 1143, 1388, 1493, 1644
HpyCH4IV	ACGT	7 cut(s)	170, 272, 562, 928, 1316, 1408, 1860
HpyF10VI	GCNNNNNNNGC	8 cut(s)	323, 332, 341, 419, 744, 1400, 1814, 1825
HpyF3I	CTNAG	3 cut(s)	657, 753, 1209
HpySE526I	ACGT	7 cut(s)	170, 272, 562, 928, 1316, 1408, 1860
HspAI	GCGC	1 cut(s)	1817
KspI	CCGCGG	2 cut(s)	1324, 1870
Kzo9I	GATC	4 cut(s)	286, 787, 997, 1800
LguI	GCTCTTC	1 cut(s)	549
LmnI	GCTCC	7 cut(s)	338, 650, 787, 1097, 1166, 1400, 1844
Lsp1109I	GCAGC	3 cut(s)	406, 665, 1276
LweI	GCATC	3 cut(s)	406, 1063, 1651
MaeI	CTAG	3 cut(s)	527, 1199, 1698
MaeII	ACGT	7 cut(s)	170, 272, 562, 928, 1316, 1408, 1860
MaeIII	GTNAC	5 cut(s)	94, 299, 910, 1105, 1613
MalI	GATC	4 cut(s)	288, 789, 999, 1802
MbiI	CCGCTC	2 cut(s)	341, 670
MboI	GATC	4 cut(s)	286, 787, 997, 1800
MflI	RGATCY	1 cut(s)	1800
MhlI	GDGCHC	5 cut(s)	110, 784, 1094, 1215, 1585
MlsI	TGGCCA	1 cut(s)	872
MluCI	AATT	2 cut(s)	444, 686
MluNI	TGGCCA	1 cut(s)	872
MlyI	GAGTC	1 cut(s)	758
MmeI	TCCRAC	4 cut(s)	157, 1415, 1723, 1812
Mox20I	TGGCCA	1 cut(s)	872
Mph1103I	ATGCAT	2 cut(s)	1666, 1670
MscI	TGGCCA	1 cut(s)	872
MseI	TTAA	3 cut(s)	645, 1226, 1709
MslI	CAYNNNNRTG	1 cut(s)	555
Msp20I	TGGCCA	1 cut(s)	872
MspA1I	CMGCKG	3 cut(s)	1217, 1323, 1869
MspI	CCGG	4 cut(s)	1452, 1702, 1804, 1854
MspR9I	CCNGG	4 cut(s)	1509, 1703, 1805, 1854
Mva1269I	GAATGC	3 cut(s)	328, 1054, 1343
MvaI	CCWGG	1 cut(s)	1509
MvnI	CGCG	3 cut(s)	1137, 1323, 1869
MwoI	GCNNNNNNNGC	8 cut(s)	323, 332, 341, 419, 744, 1400, 1814, 1825
NciI	CCSGG	3 cut(s)	1703, 1805, 1854
NcoI	CCATGG	2 cut(s)	387, 890
NdeII	GATC	4 cut(s)	286, 787, 997, 1800
NlaIV	GGNNCC	2 cut(s)	153, 1093
NmuCI	GTSAC	2 cut(s)	94, 910
NotI	GCGGCCGC	1 cut(s)	342
NsiI	ATGCAT	2 cut(s)	1666, 1670
NspI	RCATGY	5 cut(s)	216, 1539, 1651, 1668, 1828
PaeI	GCATGC	2 cut(s)	216, 1668
PciI	ACATGT	1 cut(s)	1535
PciSI	GCTCTTC	1 cut(s)	549
PctI	GAATGC	3 cut(s)	328, 1054, 1343
PfeI	GAWTC	5 cut(s)	983, 1416, 1512, 1776, 1793
PflFI	GACNNNGTC	1 cut(s)	908
PkrI	GCNGC	5 cut(s)	343, 346, 421, 655, 1291
Ple19I	CGATCG	1 cut(s)	289
PleI	GAGTC	1 cut(s)	757
PpsI	GAGTC	1 cut(s)	757
PscI	ACATGT	1 cut(s)	1535
PshBI	ATTAAT	1 cut(s)	1709
PsiI	TTATAA	1 cut(s)	1130
Psp124BI	GAGCTC	1 cut(s)	110
Psp1406I	AACGTT	1 cut(s)	170
Psp6I	CCWGG	1 cut(s)	1507
PspGI	CCWGG	1 cut(s)	1507
PspN4I	GGNNCC	2 cut(s)	153, 1093
PspPI	GGNCC	2 cut(s)	605, 835
PsrI	GAACNNNNNNTAC	2 cut(s)	1441, 1473
PstI	CTGCAG	2 cut(s)	435, 1625
PsuI	RGATCY	1 cut(s)	1800
PsyI	GACNNNGTC	1 cut(s)	908
PvuI	CGATCG	1 cut(s)	289
RseI	CAYNNNNRTG	1 cut(s)	555
SacI	GAGCTC	1 cut(s)	110
SacII	CCGCGG	2 cut(s)	1324, 1870
SapI	GCTCTTC	1 cut(s)	549
SaqAI	TTAA	3 cut(s)	645, 1226, 1709
SatI	GCNGC	5 cut(s)	342, 345, 420, 654, 1290
Sau3AI	GATC	4 cut(s)	286, 787, 997, 1800
Sau96I	GGNCC	2 cut(s)	605, 835
ScaI	AGTACT	1 cut(s)	1459
SchI	GAGTC	1 cut(s)	758
ScrFI	CCNGG	4 cut(s)	1509, 1703, 1805, 1854
SduI	GDGCHC	5 cut(s)	110, 784, 1094, 1215, 1585
SfaNI	GCATC	3 cut(s)	406, 1063, 1651
SfcI	CTRYAG	3 cut(s)	431, 1395, 1621
Sfr303I	CCGCGG	2 cut(s)	1324, 1870
SgrBI	CCGCGG	2 cut(s)	1324, 1870
SinI	GGWCC	1 cut(s)	835
SmiMI	CAYNNNNRTG	1 cut(s)	555
SmlI	CTYRAG	1 cut(s)	665
SmoI	CTYRAG	1 cut(s)	665
SphI	GCATGC	2 cut(s)	216, 1668
Sse9I	AATT	2 cut(s)	444, 686
SspI	AATATT	1 cut(s)	1113
SspMI	CTAG	3 cut(s)	527, 1199, 1698
SstI	GAGCTC	1 cut(s)	110
StyD4I	CCNGG	4 cut(s)	1507, 1701, 1803, 1852
StyI	CCWWGG	3 cut(s)	387, 890, 988
TaaI	ACNGT	9 cut(s)	184, 378, 716, 744, 779, 1143, 1388, 1493, 1644
TaiI	ACGT	7 cut(s)	173, 275, 565, 931, 1319, 1411, 1863
TaqI	TCGA	5 cut(s)	110, 285, 289, 734, 1774
TasI	AATT	2 cut(s)	444, 686
TatI	WGTACW	6 cut(s)	186, 951, 1143, 1457, 1644, 1821
TauI	GCSGC	2 cut(s)	344, 347
TfiI	GAWTC	5 cut(s)	983, 1416, 1512, 1776, 1793
Tru1I	TTAA	3 cut(s)	645, 1226, 1709
Tru9I	TTAA	3 cut(s)	645, 1226, 1709
TscAI	CASTG	8 cut(s)	749, 761, 811, 919, 1213, 1391, 1592, 1690
TseFI	GTSAC	2 cut(s)	94, 910
TseI	GCWGC	3 cut(s)	419, 653, 1289
Tsp45I	GTSAC	2 cut(s)	94, 910
TspDTI	ATGAA	7 cut(s)	17, 128, 387, 573, 891, 953, 1854
TspGWI	ACGGA	2 cut(s)	177, 836
TspRI	CASTG	8 cut(s)	749, 761, 811, 919, 1213, 1391, 1592, 1690
Tth111I	GACNNNGTC	1 cut(s)	908
VneI	GTGCAC	1 cut(s)	1581
VpaK11BI	GGWCC	1 cut(s)	835
VspI	ATTAAT	1 cut(s)	1709
XbaI	TCTAGA	1 cut(s)	526
XceI	RCATGY	5 cut(s)	216, 1539, 1651, 1668, 1828
XcmI	CCANNNNNNNNNTGG	1 cut(s)	897
XmiI	GTMKAC	1 cut(s)	1379
XspI	CTAG	3 cut(s)	527, 1199, 1698
ZrmI	AGTACT	1 cut(s)	1459
Zsp2I	ATGCAT	2 cut(s)	1666, 1670

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.