Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001680

Physical Location & Seq

Genomic Coordinates Reverse (-)

29742222 .. 29746660

Gene Structure 4439 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1220800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1317 bp

ATGGCGATAGACTCAGAACATGGAGCTTTCGTGGAGAAATTCCTTCTTCGGCCGCCGTCTTCGTCACATGACCTTCCTCTGAGCAGCCTCACCTTTGCTGTTAAAGACATATTTGATGTGGCTGGACGTGTGACTGGGTTTGGGAATCCTGATTGGGCGAGGACTCACCCGGCTGCTGAATCAACAGCTCCGGCTGTTTCGGCAATATTGAGTGGAGGTGCCACAAGTATTGGTATAACTGTCATGGATGAAATGGCTTACTGTATGAATGGAGAGAACAAGCATTATGGCACACCCAGAAATCCGTGCGCACCAGATAGGGTGCCTGGAGGCTCTTCTAGCGGTTCTGCTGTTGTCGTAGCTGCAGGCCTCGCAGATTTTTCCTTAGGAACCGATACTGGAGGAAGTGTAAGAGTTCCTGCTTCATATTGTGGAATTTTTGGGTTTCGGCCATCTCATGGCGTCATTTCTACTTCTGGAGTTACTCCTCTGGCACAAAGTTTCGATACAGTGGGATGGTTTGCAAGGAATCCTGCAATTTTGAATAGACTTGGACGAGTGTTACTCCAATTGCCTGATACGGGTCCTGTCGTACCCACTCAGTTAATCATCGCAGAAGACTGTTTCCAGATTTCAAGTGTTCCAAGCAGTCGAGTGAAACAAGTACTTGTTGACTCAGGACAAGCTCATAACATGGAAAGAAGACGCTCACTCGTTATATCTGCAAATGCAGGTCAAGTTATAAAACATGCTAACCTCGGGGATGTTGTCAAGGACAAAGTTCCAAGCTTGAACTCTTTCTTGGATAAAGGAAATCCAAGTGAAGAGTACATTATACCGTCATTGGCAGCCCTATCAACCGCCTTTCATCTTCTCGTAAGGTATGAATTCAAGAGCAACCATGGTGAATGGGTTAGTACAGTCAGACCTGACTTAGGTCCCGGGATAGGAGAACGTATATGGGCAGCCGTTCGGTCAACAGATGAAAATGTCGATGTTTGTCACTCCGTGATGACTGAATTGCGTGCTGCTCTTACTGACCTTCTCGGGGATTTCGGTCTCCTCGCAATGCCTACAGTTCCAGGGGATCCACCAAAGCTGCAAACAGATCCTACTATATTGGACGCTTTTAGTGCCAGGCTTACAGCTTCCTATCCATTGCCGTGGTTTCCGGATTCTGCCAGTATACCTCTAGGGATGTACGATAACCTACCTGTTTCAGTTTCCTTGCTGGCAAAGCATGGCTCGGACGGGTTCCTGCTCAATCTTGTGGAGACTCTTTACGACACTCTCAAAGAACACGTCAGGAAGTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004040 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006082 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009683 GO:0009684 GO:0009850 GO:0009851 GO:0009987 GO:0010817 GO:0016053 GO:0016787 GO:0016810 GO:0016811 GO:0018130 GO:0019438 GO:0019752 GO:0031974 GO:0031981 GO:0032787 GO:0034641 GO:0034754 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043864 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046483 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0072330 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120

Pfam Domains

Protein Families

Amidase

Protein Analysis

439

Amino Acids

46.64

Weight (kDa)

5.63

Isoelectric Point (pI)

36.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidase	PF01425	23 - 177	1.7e-49	Amidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001680)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08980
fragaria_vesca	FvH4_6g05640 FvH4_6g05640 FvH4_6g05640 FvH4_6g05640
malus_domestica	MD00G1147100.v1.1 MD00G1147200.v1.1 MD00G1192400.v1.1 MD04G1207000.v1.1 MD12G1220800.v1.1 MD12G1220900.v1.1 MD12G1221100.v1.1
prunus_persica	Prupe.6G327700_v2.0.a1 Prupe.6G327700_v2.0.a1
pyrus_communis	pycom04g18300 pycom12g20660 pycom12g20670 pycom12g20680 pycom12g20700 pycom12g20710 pycom12g20760
rosa_chinensis	RchiOBHm_Chr3g0454411 RchiOBHm_Chr3g0454431 RchiOBHm_Chr3g0454461 RchiOBHm_Chr3g0454491
rosa_laevigata	RLG00000025440 RLG00000025441 RLG00000025442
rosa_multiflora	Rmu_sc0007209.1_g000005 Rmu_sc0026738.1_g000001
rosa_roxburghii	Rroxscaffold_6G00425030 Rroxscaffold_6G00425050
rosa_rugosa	Rorug02G0654600 Rorug02G0654700
rosa_samantha	Rh1BG006800 Rh3AG058700 Rh3AG059100 Rh3AG059200 Rh3BG060700 Rh3BG061100 Rh3BG061300 Rh3CG059500 Rh3CG059900 Rh3CG060100 Rh3DG060800 Rh3DG061000
rosa_wichuraiana	Rw3G004570 Rw3G004580 Rw3G004590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	743
Acc16I	TGCGCA	1 cut(s)	310
Acc36I	ACCTGC	1 cut(s)	722
AccB1I	GGYRCC	2 cut(s)	218, 322
AccB7I	CCANNNNNTGG	1 cut(s)	458
AccI	GTMKAC	1 cut(s)	1186
AccIII	TCCGGA	1 cut(s)	1171
AciI	CCGC	3 cut(s)	53, 342, 861
AclWI	GGATC	3 cut(s)	1082, 1095, 1103
AcoI	YGGCCR	2 cut(s)	50, 449
AcsI	RAATTY	3 cut(s)	38, 435, 887
AcyI	GRCGYC	1 cut(s)	462
AdeI	CACNNNGTG	1 cut(s)	1009
AfaI	GTAC	5 cut(s)	594, 666, 830, 919, 1202
AfiI	CCNNNNNNNGG	5 cut(s)	458, 581, 935, 947, 1048
AflIII	ACRYGT	2 cut(s)	127, 1300
AgsI	TTSAA	4 cut(s)	544, 636, 793, 892
AjiI	CACGTC	2 cut(s)	128, 1303
AjnI	CCWGG	3 cut(s)	325, 1081, 1136
AjuI	GAANNNNNNNTTGG	2 cut(s)	785, 817
AluBI	AGCT	7 cut(s)	26, 188, 362, 686, 789, 1099, 1148
AluI	AGCT	7 cut(s)	26, 188, 362, 686, 789, 1099, 1148
Alw26I	GTCTC	2 cut(s)	1064, 1268
AlwI	GGATC	3 cut(s)	1082, 1095, 1103
Ama87I	CYCGRG	3 cut(s)	758, 941, 1046
Aor13HI	TCCGGA	1 cut(s)	1171
AoxI	GGCC	3 cut(s)	50, 367, 449
ApeKI	GCWGC	7 cut(s)	84, 173, 362, 848, 965, 1028, 1099
ApoI	RAATTY	3 cut(s)	38, 435, 887
ArsI	GACNNNNNNTTYG	1 cut(s)	1287
Asp700I	GAANNNNTTC	1 cut(s)	797
AspLEI	GCGC	1 cut(s)	311
AspS9I	GGNCC	2 cut(s)	584, 938
AsuC2I	CCSGG	3 cut(s)	170, 942, 943
AsuHPI	GGTGA	3 cut(s)	82, 158, 917
AvaI	CYCGRG	3 cut(s)	758, 941, 1046
AvaII	GGWCC	2 cut(s)	584, 938
AxyI	CCTNAGG	1 cut(s)	385
BamHI	GGATCC	1 cut(s)	1087
BanI	GGYRCC	2 cut(s)	218, 322
BbsI	GAAGAC	3 cut(s)	51, 624, 709
BbvI	GCAGC	7 cut(s)	96, 160, 349, 860, 977, 1015, 1086
BccI	CCATC	2 cut(s)	460, 510
BceAI	ACGGC	3 cut(s)	40, 953, 1147
BciT130I	CCWGG	3 cut(s)	327, 1083, 1138
BcnI	CCSGG	3 cut(s)	170, 942, 943
BcoDI	GTCTC	2 cut(s)	1064, 1268
BfaI	CTAG	2 cut(s)	339, 1193
BfmI	CTRYAG	2 cut(s)	363, 1074
BfuAI	ACCTGC	1 cut(s)	722
BisI	GCNGC	8 cut(s)	53, 85, 174, 363, 849, 966, 1029, 1100
BlsI	GCNGC	8 cut(s)	54, 86, 175, 364, 850, 967, 1030, 1101
BmcAI	AGTACT	1 cut(s)	666
Bme1390I	CCNGG	6 cut(s)	170, 327, 942, 943, 1083, 1138
Bme18I	GGWCC	2 cut(s)	584, 938
BmeT110I	CYCGRG	3 cut(s)	758, 941, 1046
BmgBI	CACGTC	2 cut(s)	128, 1303
BmgT120I	GGNCC	2 cut(s)	584, 938
BmiI	GGNNCC	7 cut(s)	220, 324, 391, 585, 940, 1089, 1256
BmrFI	CCNGG	6 cut(s)	170, 327, 942, 943, 1083, 1138
BmrI	ACTGGG	1 cut(s)	144
BmuI	ACTGGG	1 cut(s)	144
BoxI	GACNNNNGTC	1 cut(s)	936
BpiI	GAAGAC	3 cut(s)	51, 624, 709
BplI	GAGNNNNNCTC	2 cut(s)	549, 581
BpmI	CTGGAG	3 cut(s)	348, 420, 498
BpuMI	CCSGG	3 cut(s)	170, 942, 943
BsaHI	GRCGYC	1 cut(s)	462
BsaI	GGTCTC	1 cut(s)	1064
BsaJI	CCNNGG	5 cut(s)	757, 901, 941, 1082, 1163
BsaWI	WCCGGW	1 cut(s)	1171
BsaXI	ACNNNNNCTCC	4 cut(s)	15, 45, 393, 423
Bsc4I	CCNNNNNNNGG	5 cut(s)	458, 581, 935, 947, 1048
Bse1I	ACTGG	3 cut(s)	139, 403, 1182
Bse21I	CCTNAGG	1 cut(s)	385
Bse3DI	GCAATG	2 cut(s)	1074, 1157
BseAI	TCCGGA	1 cut(s)	1171
BseBI	CCWGG	3 cut(s)	327, 1083, 1138
BseDI	CCNNGG	5 cut(s)	757, 901, 941, 1082, 1163
BseGI	GGATG	4 cut(s)	253, 521, 769, 1203
BseLI	CCNNNNNNNGG	5 cut(s)	458, 581, 935, 947, 1048
BseMI	GCAATG	2 cut(s)	1074, 1157
BseMII	CTCAG	4 cut(s)	27, 71, 614, 690
BseNI	ACTGG	3 cut(s)	139, 403, 1182
BseRI	GAGGAG	2 cut(s)	477, 1052
BseX3I	CGGCCG	1 cut(s)	50
BseXI	GCAGC	7 cut(s)	96, 160, 349, 860, 977, 1015, 1086
Bsh1285I	CGRYCG	1 cut(s)	53
BshFI	GGCC	3 cut(s)	52, 369, 451
BshNI	GGYRCC	2 cut(s)	218, 322
BsiEI	CGRYCG	1 cut(s)	53
BsiHKCI	CYCGRG	3 cut(s)	758, 941, 1046
BsiSI	CCGG	4 cut(s)	170, 191, 942, 1172
BslFI	GGGAC	1 cut(s)	924
BslI	CCNNNNNNNGG	5 cut(s)	458, 581, 935, 947, 1048
BsmAI	GTCTC	2 cut(s)	1064, 1268
BsmFI	GGGAC	1 cut(s)	924
BsnI	GGCC	3 cut(s)	52, 369, 451
Bso31I	GGTCTC	1 cut(s)	1064
BsoBI	CYCGRG	3 cut(s)	758, 941, 1046
Bsp13I	TCCGGA	1 cut(s)	1171
Bsp143I	GATC	2 cut(s)	1087, 1108
Bsp19I	CCATGG	1 cut(s)	901
BspACI	CCGC	3 cut(s)	53, 342, 861
BspANI	GGCC	3 cut(s)	52, 369, 451
BspCNI	CTCAG	4 cut(s)	26, 72, 613, 689
BspEI	TCCGGA	1 cut(s)	1171
BspLI	GGNNCC	7 cut(s)	220, 324, 391, 585, 940, 1089, 1256
BspMAI	CTGCAG	1 cut(s)	367
BspMI	ACCTGC	1 cut(s)	722
BspPI	GGATC	3 cut(s)	1082, 1095, 1103
BspQI	GCTCTTC	1 cut(s)	340
BspT107I	GGYRCC	2 cut(s)	218, 322
BspTNI	GGTCTC	1 cut(s)	1064
BsrDI	GCAATG	2 cut(s)	1074, 1157
BsrI	ACTGG	3 cut(s)	139, 403, 1182
BssECI	CCNNGG	5 cut(s)	757, 901, 941, 1082, 1163
BssMI	GATC	2 cut(s)	1087, 1108
BssNAI	GTATAC	1 cut(s)	1187
BssNI	GRCGYC	1 cut(s)	462
BssT1I	CCWWGG	1 cut(s)	901
Bst1107I	GTATAC	1 cut(s)	1187
Bst2UI	CCWGG	3 cut(s)	327, 1083, 1138
Bst4CI	ACNGT	7 cut(s)	241, 263, 511, 623, 840, 922, 1078
Bst6I	CTCTTC	2 cut(s)	340, 819
BstACI	GRCGYC	1 cut(s)	462
BstC8I	GCNNGC	3 cut(s)	367, 1026, 1233
BstDEI	CTNAG	6 cut(s)	13, 80, 385, 600, 676, 934
BstDSI	CCRYGG	2 cut(s)	901, 1163
BstENI	CCTNNNNNAGG	1 cut(s)	933
BstF5I	GGATG	4 cut(s)	253, 521, 769, 1203
BstHHI	GCGC	1 cut(s)	311
BstKTI	GATC	2 cut(s)	1090, 1111
BstMAI	GTCTC	2 cut(s)	1064, 1268
BstMBI	GATC	2 cut(s)	1087, 1108
BstMCI	CGRYCG	1 cut(s)	53
BstMWI	GCNNNNNNNGC	5 cut(s)	200, 339, 371, 1133, 1237
BstNI	CCWGG	3 cut(s)	327, 1083, 1138
BstNSI	RCATGY	1 cut(s)	752
BstPAI	GACNNNNGTC	1 cut(s)	936
BstSCI	CCNGG	6 cut(s)	168, 325, 940, 941, 1081, 1136
BstSFI	CTRYAG	2 cut(s)	363, 1074
BstV1I	GCAGC	7 cut(s)	96, 160, 349, 860, 977, 1015, 1086
BstV2I	GAAGAC	3 cut(s)	51, 624, 709
BstX2I	RGATCY	2 cut(s)	1087, 1108
BstXI	CCANNNNNNTGG	1 cut(s)	1164
BstYI	RGATCY	2 cut(s)	1087, 1108
BstZ17I	GTATAC	1 cut(s)	1187
BstZI	CGGCCG	1 cut(s)	50
Bsu36I	CCTNAGG	1 cut(s)	385
BsuRI	GGCC	3 cut(s)	52, 369, 451
BtgI	CCRYGG	2 cut(s)	901, 1163
BtgZI	GCGATG	1 cut(s)	595
BtrI	CACGTC	2 cut(s)	128, 1303
BtsCI	GGATG	4 cut(s)	253, 521, 769, 1203
BtsIMutI	CAGTG	1 cut(s)	516
BveI	ACCTGC	1 cut(s)	722
Cac8I	GCNNGC	3 cut(s)	367, 1026, 1233
CfoI	GCGC	1 cut(s)	311
Cfr13I	GGNCC	2 cut(s)	584, 938
Cfr9I	CCCGGG	1 cut(s)	941
CseI	GACGC	3 cut(s)	451, 714, 1133
Csp6I	GTAC	5 cut(s)	593, 665, 829, 918, 1201
CspCI	CAANNNNNGTGG	2 cut(s)	211, 246
CviAII	CATG	8 cut(s)	20, 68, 244, 458, 694, 749, 902, 1241
CviQI	GTAC	5 cut(s)	593, 665, 829, 918, 1201
DdeI	CTNAG	6 cut(s)	13, 80, 385, 600, 676, 934
DpnI	GATC	2 cut(s)	1089, 1110
DpnII	GATC	2 cut(s)	1087, 1108
DraIII	CACNNNGTG	1 cut(s)	1009
EaeI	YGGCCR	2 cut(s)	50, 449
EagI	CGGCCG	1 cut(s)	50
Eam1104I	CTCTTC	2 cut(s)	340, 819
EarI	CTCTTC	2 cut(s)	340, 819
EclXI	CGGCCG	1 cut(s)	50
Eco130I	CCWWGG	1 cut(s)	901
Eco147I	AGGCCT	1 cut(s)	369
Eco31I	GGTCTC	1 cut(s)	1064
Eco47I	GGWCC	2 cut(s)	584, 938
Eco52I	CGGCCG	1 cut(s)	50
Eco81I	CCTNAGG	1 cut(s)	385
Eco88I	CYCGRG	3 cut(s)	758, 941, 1046
EcoNI	CCTNNNNNAGG	1 cut(s)	933
EcoO109I	RGGNCCY	2 cut(s)	584, 938
EcoRI	GAATTC	1 cut(s)	887
EcoRII	CCWGG	3 cut(s)	325, 1081, 1136
EcoT14I	CCWWGG	1 cut(s)	901
ErhI	CCWWGG	1 cut(s)	901
FaeI	CATG	8 cut(s)	23, 71, 247, 461, 697, 752, 905, 1244
FaqI	GGGAC	1 cut(s)	924
FatI	CATG	8 cut(s)	19, 67, 243, 457, 693, 748, 901, 1240
FblI	GTMKAC	1 cut(s)	1186
Fnu4HI	GCNGC	8 cut(s)	53, 85, 174, 363, 849, 966, 1029, 1100
FokI	GGATG	4 cut(s)	260, 528, 776, 1210
Fsp4HI	GCNGC	8 cut(s)	53, 85, 174, 363, 849, 966, 1029, 1100
FspAI	RTGCGCAY	1 cut(s)	310
FspBI	CTAG	2 cut(s)	339, 1193
FspI	TGCGCA	1 cut(s)	310
GlaI	GCGC	1 cut(s)	310
GluI	GCNGC	8 cut(s)	53, 85, 174, 363, 849, 966, 1029, 1100
GsuI	CTGGAG	3 cut(s)	348, 420, 498
HaeIII	GGCC	3 cut(s)	52, 369, 451
HapII	CCGG	4 cut(s)	170, 191, 942, 1172
HgaI	GACGC	3 cut(s)	451, 714, 1133
HhaI	GCGC	1 cut(s)	311
Hin1I	GRCGYC	1 cut(s)	462
Hin1II	CATG	8 cut(s)	23, 71, 247, 461, 697, 752, 905, 1244
Hin6I	GCGC	1 cut(s)	309
HinP1I	GCGC	1 cut(s)	309
HincII	GTYRAC	2 cut(s)	673, 978
HindII	GTYRAC	2 cut(s)	673, 978
HindIII	AAGCTT	1 cut(s)	787
HinfI	GANTC	8 cut(s)	11, 145, 163, 179, 529, 674, 1175, 1276
HpaII	CCGG	4 cut(s)	170, 191, 942, 1172
HphI	GGTGA	3 cut(s)	82, 158, 917
Hpy166II	GTNNAC	3 cut(s)	673, 978, 1187
Hpy188I	TCNGA	4 cut(s)	16, 81, 926, 1249
Hpy188III	TCNNGA	7 cut(s)	149, 477, 628, 678, 892, 1172, 1306
Hpy8I	GTNNAC	3 cut(s)	673, 978, 1187
HpyAV	CCTTC	3 cut(s)	53, 83, 1052
HpyCH4III	ACNGT	7 cut(s)	241, 263, 511, 623, 840, 922, 1078
HpyCH4IV	ACGT	3 cut(s)	127, 955, 1302
HpyCH4V	TGCA	6 cut(s)	365, 524, 536, 725, 731, 1102
HpyF10VI	GCNNNNNNNGC	5 cut(s)	200, 339, 371, 1133, 1237
HpyF3I	CTNAG	6 cut(s)	13, 80, 385, 600, 676, 934
HpySE526I	ACGT	3 cut(s)	127, 955, 1302
Hsp92I	GRCGYC	1 cut(s)	462
Hsp92II	CATG	8 cut(s)	23, 71, 247, 461, 697, 752, 905, 1244
HspAI	GCGC	1 cut(s)	309
Kpn2I	TCCGGA	1 cut(s)	1171
Kzo9I	GATC	2 cut(s)	1087, 1108
LguI	GCTCTTC	1 cut(s)	340
LmnI	GCTCC	2 cut(s)	23, 193
Lsp1109I	GCAGC	7 cut(s)	96, 160, 349, 860, 977, 1015, 1086
MaeI	CTAG	2 cut(s)	339, 1193
MaeII	ACGT	3 cut(s)	127, 955, 1302
MaeIII	GTNAC	5 cut(s)	63, 130, 481, 561, 1001
MalI	GATC	2 cut(s)	1089, 1110
MboI	GATC	2 cut(s)	1087, 1108
MboII	GAAGA	7 cut(s)	38, 51, 327, 629, 714, 836, 863
MfeI	CAATTG	1 cut(s)	569
MflI	RGATCY	2 cut(s)	1087, 1108
MluCI	AATT	6 cut(s)	38, 435, 537, 569, 887, 1019
MlyI	GAGTC	4 cut(s)	5, 157, 668, 1270
MroI	TCCGGA	1 cut(s)	1171
MroXI	GAANNNNTTC	1 cut(s)	797
MseI	TTAA	2 cut(s)	102, 605
MslI	CAYNNNNRTG	1 cut(s)	1162
MspI	CCGG	4 cut(s)	170, 191, 942, 1172
MspR9I	CCNGG	6 cut(s)	170, 327, 942, 943, 1083, 1138
MunI	CAATTG	1 cut(s)	569
MvaI	CCWGG	3 cut(s)	327, 1083, 1138
MwoI	GCNNNNNNNGC	5 cut(s)	200, 339, 371, 1133, 1237
NciI	CCSGG	3 cut(s)	170, 942, 943
NcoI	CCATGG	1 cut(s)	901
NdeII	GATC	2 cut(s)	1087, 1108
NlaIII	CATG	8 cut(s)	23, 71, 247, 461, 697, 752, 905, 1244
NlaIV	GGNNCC	7 cut(s)	220, 324, 391, 585, 940, 1089, 1256
NmuCI	GTSAC	3 cut(s)	63, 130, 1001
NsbI	TGCGCA	1 cut(s)	310
NspI	RCATGY	1 cut(s)	752
PceI	AGGCCT	1 cut(s)	369
PciSI	GCTCTTC	1 cut(s)	340
PdmI	GAANNNNTTC	1 cut(s)	797
PfeI	GAWTC	4 cut(s)	145, 179, 529, 1175
PflMI	CCANNNNNTGG	1 cut(s)	458
PkrI	GCNGC	8 cut(s)	54, 86, 175, 364, 850, 967, 1030, 1101
PleI	GAGTC	4 cut(s)	5, 157, 668, 1270
PpsI	GAGTC	4 cut(s)	5, 157, 668, 1270
PpuMI	RGGWCCY	2 cut(s)	584, 938
PshAI	GACNNNNGTC	1 cut(s)	936
PsiI	TTATAA	1 cut(s)	743
Psp5II	RGGWCCY	2 cut(s)	584, 938
Psp6I	CCWGG	3 cut(s)	325, 1081, 1136
PspGI	CCWGG	3 cut(s)	325, 1081, 1136
PspN4I	GGNNCC	7 cut(s)	220, 324, 391, 585, 940, 1089, 1256
PspPI	GGNCC	2 cut(s)	584, 938
PspPPI	RGGWCCY	2 cut(s)	584, 938
PstI	CTGCAG	1 cut(s)	367
PsuI	RGATCY	2 cut(s)	1087, 1108
RsaI	GTAC	5 cut(s)	594, 666, 830, 919, 1202
RsaNI	GTAC	5 cut(s)	593, 665, 829, 918, 1201
RseI	CAYNNNNRTG	1 cut(s)	1162
SapI	GCTCTTC	1 cut(s)	340
SaqAI	TTAA	2 cut(s)	102, 605
SatI	GCNGC	8 cut(s)	53, 85, 174, 363, 849, 966, 1029, 1100
Sau3AI	GATC	2 cut(s)	1087, 1108
Sau96I	GGNCC	2 cut(s)	584, 938
ScaI	AGTACT	1 cut(s)	666
SchI	GAGTC	4 cut(s)	5, 157, 668, 1270
ScrFI	CCNGG	6 cut(s)	170, 327, 942, 943, 1083, 1138
SfcI	CTRYAG	2 cut(s)	363, 1074
SinI	GGWCC	2 cut(s)	584, 938
SmaI	CCCGGG	1 cut(s)	943
SmiMI	CAYNNNNRTG	1 cut(s)	1162
Sse9I	AATT	6 cut(s)	38, 435, 537, 569, 887, 1019
SseBI	AGGCCT	1 cut(s)	369
SsiI	CCGC	3 cut(s)	53, 342, 861
SspI	AATATT	1 cut(s)	207
SspMI	CTAG	2 cut(s)	339, 1193
StuI	AGGCCT	1 cut(s)	369
StyD4I	CCNGG	6 cut(s)	168, 325, 940, 941, 1081, 1136
StyI	CCWWGG	1 cut(s)	901
TaaI	ACNGT	7 cut(s)	241, 263, 511, 623, 840, 922, 1078
TaiI	ACGT	3 cut(s)	130, 958, 1305
TaqI	TCGA	3 cut(s)	504, 652, 993
TaqII	GACCGA	2 cut(s)	963, 1046
TasI	AATT	6 cut(s)	38, 435, 537, 569, 887, 1019
TatI	WGTACW	3 cut(s)	664, 828, 917
TauI	GCSGC	1 cut(s)	55
TfiI	GAWTC	4 cut(s)	145, 179, 529, 1175
Tru1I	TTAA	2 cut(s)	102, 605
Tru9I	TTAA	2 cut(s)	102, 605
TscAI	CASTG	1 cut(s)	516
TseFI	GTSAC	3 cut(s)	63, 130, 1001
TseI	GCWGC	7 cut(s)	84, 173, 362, 848, 965, 1028, 1099
Tsp45I	GTSAC	3 cut(s)	63, 130, 1001
TspDTI	ATGAA	6 cut(s)	264, 281, 414, 857, 900, 999
TspGWI	ACGGA	2 cut(s)	294, 997
TspMI	CCCGGG	1 cut(s)	941
TspRI	CASTG	1 cut(s)	516
Van91I	CCANNNNNTGG	1 cut(s)	458
VpaK11BI	GGWCC	2 cut(s)	584, 938
XagI	CCTNNNNNAGG	1 cut(s)	933
XapI	RAATTY	3 cut(s)	38, 435, 887
XceI	RCATGY	1 cut(s)	752
XmaI	CCCGGG	1 cut(s)	941
XmiI	GTMKAC	1 cut(s)	1186
XmnI	GAANNNNTTC	1 cut(s)	797
XspI	CTAG	2 cut(s)	339, 1193
ZrmI	AGTACT	1 cut(s)	666

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.