Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001680

Physical Location & Seq

Genomic Coordinates Reverse (-)

29754118 .. 29757538

Gene Structure 3421 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1221100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1287 bp

ATGGCGATAGACTCAGATCATGGAGCTTTCGTGGAGAAATTCCTTCTCCGACCGCCGTCTTCATCACATGACCTTCCTCTGAGTGGCCTCACCTTTGCTGTTAAAGACATATTTGATGTGGCTGGACGTGTGACTGGGTTTGGGAATCCTGATTGGGCGAGGACTCACCCGGCTGCTGAATCAACAGCTCCGGCTGTTTCAGCAATATTGAGTGGAGGTGCCACAAGTATTGGTATAACTGTCATGGATGAAATGGCATACTGTATGAATGGAGAGAACAAGCATTATGGCACACCCAGAAATCCGTGCGCTCCAGATAGGGTGCCTGGAGGCTCTTCTAGCGGTTCTGCTGTTGCCGTAGCTGTAGGCCTCGCAGATTTTTCCTTAGGAACTGACACTGGAGGAAGTGTAAGAATTCCTGCGTCGCATTGTGGAATTTTTGGGTTTCGGCCATCTCATGGTGTCATTTCTACTTCTGGAGTTACTCCTCTGGCACAAAGTTTCGATACAGTGGGATGGTTTGCAAGGAATCCTGCGATTTTGAATAGAGTTGGACGAGTGTTACTCCAATTGCCTGATACGGGTCCTGTCGTACCCACTCAGTTAATCATCGCAGAAGACTGTTTCCAGATTTCAAGTGTTCCAAGCAGTCGAGTGAAACAAGTACTTGTTGAGTCAGTTGAGAAGTTATTTGGGGGTCAAGTTATAAAACATGCTAACCTCGGGGATGTTGTCAAGGACAAAGTTCCAAGCTTGAACTCTTTCTTGGATAAAGGAAATCCAAGTGAAGAGTACATTATACCGTCTTTGGCAGCCCTATCAACCGCCTTTCATCTTCTCGCAAGGTATGAATTCAAGAGCAACCATGGTGAATGGGTTAGTACAGTCAGACCTGACTTAGGTCCCGGGATAGGAGAACGTATATGGGCAGCCGTTCGGTCAACAGATGAAAATGTCGATGTTTGTCACTCCGTGATGACTGAATTGCGTGCTGCTCTTACTGAACTTCTCGGGGATTTCGGTGTCCTCGCAATGCCTACAGTTCCAGGGGATCCACCAAAGCTGCAAACAGATCCTAGTATACTGGACGCTTTTCGTGCCCGGGCTTTCAGCTTCCAATCCATTGCCGTAGTTTCCGGATTCTGCCAGGTGAGTATACCACTAGGGATGTGCGATAACCTACCTGTTTCAGTTTCCTTGCTGGCAAAGCATGGCTCGGATGGATTCCTGCTCAATCTTGTGGAGACTCTTTACGGCACTCTCAAAGAACACGCCGGGAAGTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004040 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006082 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009683 GO:0009684 GO:0009850 GO:0009851 GO:0009987 GO:0010817 GO:0016053 GO:0016787 GO:0016810 GO:0016811 GO:0018130 GO:0019438 GO:0019752 GO:0031974 GO:0031981 GO:0032787 GO:0034641 GO:0034754 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043864 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046483 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0072330 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120

Pfam Domains

Protein Families

Amidase

Protein Analysis

429

Amino Acids

45.17

Weight (kDa)

5.63

Isoelectric Point (pI)

30.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidase	PF01425	23 - 177	2.6e-50	Amidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001680)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08980
fragaria_vesca	FvH4_6g05640 FvH4_6g05640 FvH4_6g05640 FvH4_6g05640
malus_domestica	MD00G1147100.v1.1 MD00G1147200.v1.1 MD00G1192400.v1.1 MD04G1207000.v1.1 MD12G1220800.v1.1 MD12G1220900.v1.1 MD12G1221100.v1.1
prunus_persica	Prupe.6G327700_v2.0.a1 Prupe.6G327700_v2.0.a1
pyrus_communis	pycom04g18300 pycom12g20660 pycom12g20670 pycom12g20680 pycom12g20700 pycom12g20710 pycom12g20760
rosa_chinensis	RchiOBHm_Chr3g0454411 RchiOBHm_Chr3g0454431 RchiOBHm_Chr3g0454461 RchiOBHm_Chr3g0454491
rosa_laevigata	RLG00000025440 RLG00000025441 RLG00000025442
rosa_multiflora	Rmu_sc0007209.1_g000005 Rmu_sc0026738.1_g000001
rosa_roxburghii	Rroxscaffold_6G00425030 Rroxscaffold_6G00425050
rosa_rugosa	Rorug02G0654600 Rorug02G0654700
rosa_samantha	Rh1BG006800 Rh3AG058700 Rh3AG059100 Rh3AG059200 Rh3BG060700 Rh3BG061100 Rh3BG061300 Rh3CG059500 Rh3CG059900 Rh3CG060100 Rh3DG060800 Rh3DG061000
rosa_wichuraiana	Rw3G004570 Rw3G004580 Rw3G004590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	707
AccB1I	GGYRCC	2 cut(s)	218, 322
AccB7I	CCANNNNNTGG	1 cut(s)	458
AccI	GTMKAC	2 cut(s)	1081, 1156
AccIII	TCCGGA	1 cut(s)	1136
AciI	CCGC	3 cut(s)	53, 342, 825
AclWI	GGATC	3 cut(s)	1046, 1059, 1067
AcoI	YGGCCR	1 cut(s)	449
AcsI	RAATTY	4 cut(s)	38, 414, 435, 851
AdeI	CACNNNGTG	1 cut(s)	973
AfaI	GTAC	4 cut(s)	594, 666, 794, 883
AfiI	CCNNNNNNNGG	5 cut(s)	83, 458, 581, 899, 911
AflIII	ACRYGT	1 cut(s)	127
AgsI	TTSAA	4 cut(s)	544, 636, 757, 856
AjiI	CACGTC	1 cut(s)	128
AjnI	CCWGG	3 cut(s)	325, 1045, 1146
AjuI	GAANNNNNNNTTGG	2 cut(s)	749, 781
AluBI	AGCT	6 cut(s)	26, 188, 362, 753, 1063, 1113
AluI	AGCT	6 cut(s)	26, 188, 362, 753, 1063, 1113
Alw26I	GTCTC	1 cut(s)	1238
AlwI	GGATC	3 cut(s)	1046, 1059, 1067
Ama87I	CYCGRG	4 cut(s)	722, 905, 1010, 1101
Aor13HI	TCCGGA	1 cut(s)	1136
AoxI	GGCC	3 cut(s)	85, 367, 449
ApeKI	GCWGC	5 cut(s)	173, 812, 929, 992, 1063
ApoI	RAATTY	4 cut(s)	38, 414, 435, 851
Asp700I	GAANNNNTTC	1 cut(s)	761
AspLEI	GCGC	1 cut(s)	311
AspS9I	GGNCC	2 cut(s)	584, 902
AsuC2I	CCSGG	6 cut(s)	170, 906, 907, 1102, 1103, 1276
AsuHPI	GGTGA	4 cut(s)	82, 158, 881, 1162
AvaI	CYCGRG	4 cut(s)	722, 905, 1010, 1101
AvaII	GGWCC	2 cut(s)	584, 902
AxyI	CCTNAGG	1 cut(s)	385
BaeGI	GKGCMC	1 cut(s)	1102
BamHI	GGATCC	1 cut(s)	1051
BanI	GGYRCC	2 cut(s)	218, 322
BbsI	GAAGAC	2 cut(s)	51, 624
BbvI	GCAGC	5 cut(s)	160, 824, 941, 979, 1050
BccI	CCATC	3 cut(s)	460, 510, 1214
BceAI	ACGGC	5 cut(s)	40, 341, 917, 1112, 1270
BciT130I	CCWGG	3 cut(s)	327, 1047, 1148
BcnI	CCSGG	6 cut(s)	170, 906, 907, 1102, 1103, 1276
BcoDI	GTCTC	1 cut(s)	1238
BfaI	CTAG	3 cut(s)	339, 1077, 1163
BfmI	CTRYAG	2 cut(s)	363, 1038
BisI	GCNGC	5 cut(s)	174, 813, 930, 993, 1064
BlsI	GCNGC	5 cut(s)	175, 814, 931, 994, 1065
BmcAI	AGTACT	1 cut(s)	666
Bme1390I	CCNGG	9 cut(s)	170, 327, 906, 907, 1047, 1102, 1103, 1148, 1276
Bme18I	GGWCC	2 cut(s)	584, 902
BmeT110I	CYCGRG	4 cut(s)	722, 905, 1010, 1101
BmgBI	CACGTC	1 cut(s)	128
BmgT120I	GGNCC	2 cut(s)	584, 902
BmiI	GGNNCC	5 cut(s)	220, 324, 585, 904, 1053
BmrFI	CCNGG	9 cut(s)	170, 327, 906, 907, 1047, 1102, 1103, 1148, 1276
BmrI	ACTGGG	1 cut(s)	144
BmuI	ACTGGG	1 cut(s)	144
BoxI	GACNNNNGTC	2 cut(s)	55, 900
BpiI	GAAGAC	2 cut(s)	51, 624
BplI	GAGNNNNNCTC	2 cut(s)	549, 581
BpmI	CTGGAG	4 cut(s)	297, 348, 420, 498
BpuMI	CCSGG	6 cut(s)	170, 906, 907, 1102, 1103, 1276
BsaJI	CCNNGG	5 cut(s)	721, 865, 905, 1046, 1101
BsaWI	WCCGGW	1 cut(s)	1136
BsaXI	ACNNNNNCTCC	4 cut(s)	15, 45, 393, 423
Bsc4I	CCNNNNNNNGG	5 cut(s)	83, 458, 581, 899, 911
Bse1I	ACTGG	3 cut(s)	139, 403, 1089
Bse21I	CCTNAGG	1 cut(s)	385
Bse3DI	GCAATG	2 cut(s)	1038, 1122
BseAI	TCCGGA	1 cut(s)	1136
BseBI	CCWGG	3 cut(s)	327, 1047, 1148
BseDI	CCNNGG	5 cut(s)	721, 865, 905, 1046, 1101
BseGI	GGATG	5 cut(s)	253, 521, 733, 1173, 1225
BseLI	CCNNNNNNNGG	5 cut(s)	83, 458, 581, 899, 911
BseMI	GCAATG	2 cut(s)	1038, 1122
BseMII	CTCAG	3 cut(s)	27, 71, 614
BseNI	ACTGG	3 cut(s)	139, 403, 1089
BseRI	GAGGAG	1 cut(s)	477
BseSI	GKGCMC	1 cut(s)	1102
BseXI	GCAGC	5 cut(s)	160, 824, 941, 979, 1050
Bsh1285I	CGRYCG	1 cut(s)	53
BshFI	GGCC	3 cut(s)	87, 369, 451
BshNI	GGYRCC	2 cut(s)	218, 322
BsiEI	CGRYCG	1 cut(s)	53
BsiHKCI	CYCGRG	4 cut(s)	722, 905, 1010, 1101
BsiSI	CCGG	6 cut(s)	170, 191, 906, 1102, 1137, 1275
BslFI	GGGAC	1 cut(s)	888
BslI	CCNNNNNNNGG	5 cut(s)	83, 458, 581, 899, 911
BsmAI	GTCTC	1 cut(s)	1238
BsmFI	GGGAC	1 cut(s)	888
BsnI	GGCC	3 cut(s)	87, 369, 451
BsoBI	CYCGRG	4 cut(s)	722, 905, 1010, 1101
Bsp1286I	GDGCHC	1 cut(s)	1102
Bsp13I	TCCGGA	1 cut(s)	1136
Bsp143I	GATC	3 cut(s)	16, 1051, 1072
Bsp19I	CCATGG	1 cut(s)	865
BspACI	CCGC	3 cut(s)	53, 342, 825
BspANI	GGCC	3 cut(s)	87, 369, 451
BspCNI	CTCAG	3 cut(s)	26, 72, 613
BspEI	TCCGGA	1 cut(s)	1136
BspLI	GGNNCC	5 cut(s)	220, 324, 585, 904, 1053
BspPI	GGATC	3 cut(s)	1046, 1059, 1067
BspQI	GCTCTTC	1 cut(s)	340
BspT107I	GGYRCC	2 cut(s)	218, 322
BsrDI	GCAATG	2 cut(s)	1038, 1122
BsrI	ACTGG	3 cut(s)	139, 403, 1089
BssECI	CCNNGG	5 cut(s)	721, 865, 905, 1046, 1101
BssMI	GATC	3 cut(s)	16, 1051, 1072
BssNAI	GTATAC	2 cut(s)	1082, 1157
BssT1I	CCWWGG	1 cut(s)	865
Bst1107I	GTATAC	2 cut(s)	1082, 1157
Bst2UI	CCWGG	3 cut(s)	327, 1047, 1148
Bst4CI	ACNGT	7 cut(s)	241, 263, 511, 623, 804, 886, 1042
Bst6I	CTCTTC	2 cut(s)	340, 783
BstC8I	GCNNGC	2 cut(s)	990, 1203
BstDEI	CTNAG	5 cut(s)	13, 80, 385, 600, 898
BstDSI	CCRYGG	1 cut(s)	865
BstENI	CCTNNNNNAGG	1 cut(s)	897
BstF5I	GGATG	5 cut(s)	253, 521, 733, 1173, 1225
BstHHI	GCGC	1 cut(s)	311
BstKTI	GATC	3 cut(s)	19, 1054, 1075
BstMAI	GTCTC	1 cut(s)	1238
BstMBI	GATC	3 cut(s)	16, 1051, 1072
BstMCI	CGRYCG	1 cut(s)	53
BstMWI	GCNNNNNNNGC	4 cut(s)	200, 339, 1097, 1207
BstNI	CCWGG	3 cut(s)	327, 1047, 1148
BstNSI	RCATGY	1 cut(s)	716
BstPAI	GACNNNNGTC	2 cut(s)	55, 900
BstSCI	CCNGG	9 cut(s)	168, 325, 904, 905, 1045, 1100, 1101, 1146, 1274
BstSFI	CTRYAG	2 cut(s)	363, 1038
BstSLI	GKGCMC	1 cut(s)	1102
BstV1I	GCAGC	5 cut(s)	160, 824, 941, 979, 1050
BstV2I	GAAGAC	2 cut(s)	51, 624
BstX2I	RGATCY	2 cut(s)	1051, 1072
BstYI	RGATCY	2 cut(s)	1051, 1072
BstZ17I	GTATAC	2 cut(s)	1082, 1157
Bsu36I	CCTNAGG	1 cut(s)	385
BsuRI	GGCC	3 cut(s)	87, 369, 451
BtgI	CCRYGG	1 cut(s)	865
BtgZI	GCGATG	1 cut(s)	595
BtrI	CACGTC	1 cut(s)	128
BtsCI	GGATG	5 cut(s)	253, 521, 733, 1173, 1225
BtsIMutI	CAGTG	2 cut(s)	396, 516
Cac8I	GCNNGC	2 cut(s)	990, 1203
CfoI	GCGC	1 cut(s)	311
Cfr13I	GGNCC	2 cut(s)	584, 902
Cfr9I	CCCGGG	2 cut(s)	905, 1101
CseI	GACGC	2 cut(s)	411, 1097
Csp6I	GTAC	4 cut(s)	593, 665, 793, 882
CspCI	CAANNNNNGTGG	2 cut(s)	211, 246
CviAII	CATG	7 cut(s)	20, 68, 244, 458, 713, 866, 1211
CviQI	GTAC	4 cut(s)	593, 665, 793, 882
DdeI	CTNAG	5 cut(s)	13, 80, 385, 600, 898
DpnI	GATC	3 cut(s)	18, 1053, 1074
DpnII	GATC	3 cut(s)	16, 1051, 1072
DraIII	CACNNNGTG	1 cut(s)	973
EaeI	YGGCCR	1 cut(s)	449
Eam1104I	CTCTTC	2 cut(s)	340, 783
EarI	CTCTTC	2 cut(s)	340, 783
Eco130I	CCWWGG	1 cut(s)	865
Eco147I	AGGCCT	1 cut(s)	369
Eco47I	GGWCC	2 cut(s)	584, 902
Eco81I	CCTNAGG	1 cut(s)	385
Eco88I	CYCGRG	4 cut(s)	722, 905, 1010, 1101
EcoNI	CCTNNNNNAGG	1 cut(s)	897
EcoO109I	RGGNCCY	2 cut(s)	584, 902
EcoRI	GAATTC	2 cut(s)	414, 851
EcoRII	CCWGG	3 cut(s)	325, 1045, 1146
EcoT14I	CCWWGG	1 cut(s)	865
ErhI	CCWWGG	1 cut(s)	865
FaeI	CATG	7 cut(s)	23, 71, 247, 461, 716, 869, 1214
FaqI	GGGAC	1 cut(s)	888
FatI	CATG	7 cut(s)	19, 67, 243, 457, 712, 865, 1210
FblI	GTMKAC	2 cut(s)	1081, 1156
Fnu4HI	GCNGC	5 cut(s)	174, 813, 930, 993, 1064
FokI	GGATG	5 cut(s)	260, 528, 740, 1180, 1232
Fsp4HI	GCNGC	5 cut(s)	174, 813, 930, 993, 1064
FspBI	CTAG	3 cut(s)	339, 1077, 1163
GlaI	GCGC	1 cut(s)	310
GluI	GCNGC	5 cut(s)	174, 813, 930, 993, 1064
GsuI	CTGGAG	4 cut(s)	297, 348, 420, 498
HaeIII	GGCC	3 cut(s)	87, 369, 451
HapII	CCGG	6 cut(s)	170, 191, 906, 1102, 1137, 1275
HgaI	GACGC	2 cut(s)	411, 1097
HhaI	GCGC	1 cut(s)	311
Hin1II	CATG	7 cut(s)	23, 71, 247, 461, 716, 869, 1214
Hin6I	GCGC	1 cut(s)	309
HinP1I	GCGC	1 cut(s)	309
HincII	GTYRAC	1 cut(s)	942
HindII	GTYRAC	1 cut(s)	942
HindIII	AAGCTT	1 cut(s)	751
HinfI	GANTC	9 cut(s)	11, 145, 163, 179, 529, 674, 1140, 1224, 1246
HpaII	CCGG	6 cut(s)	170, 191, 906, 1102, 1137, 1275
HphI	GGTGA	4 cut(s)	82, 158, 881, 1162
Hpy166II	GTNNAC	3 cut(s)	942, 1082, 1157
Hpy188I	TCNGA	5 cut(s)	16, 50, 81, 890, 1219
Hpy188III	TCNNGA	6 cut(s)	149, 314, 477, 628, 856, 1137
Hpy8I	GTNNAC	3 cut(s)	942, 1082, 1157
Hpy99I	CGWCG	1 cut(s)	427
HpyAV	CCTTC	2 cut(s)	53, 83
HpyCH4III	ACNGT	7 cut(s)	241, 263, 511, 623, 804, 886, 1042
HpyCH4IV	ACGT	2 cut(s)	127, 919
HpyCH4V	TGCA	2 cut(s)	524, 1066
HpyF10VI	GCNNNNNNNGC	4 cut(s)	200, 339, 1097, 1207
HpyF3I	CTNAG	5 cut(s)	13, 80, 385, 600, 898
HpySE526I	ACGT	2 cut(s)	127, 919
Hsp92II	CATG	7 cut(s)	23, 71, 247, 461, 716, 869, 1214
HspAI	GCGC	1 cut(s)	309
Kpn2I	TCCGGA	1 cut(s)	1136
Kzo9I	GATC	3 cut(s)	16, 1051, 1072
LguI	GCTCTTC	1 cut(s)	340
LmnI	GCTCC	3 cut(s)	23, 193, 316
Lsp1109I	GCAGC	5 cut(s)	160, 824, 941, 979, 1050
MaeI	CTAG	3 cut(s)	339, 1077, 1163
MaeII	ACGT	2 cut(s)	127, 919
MaeIII	GTNAC	4 cut(s)	130, 481, 561, 965
MalI	GATC	3 cut(s)	18, 1053, 1074
MboI	GATC	3 cut(s)	16, 1051, 1072
MboII	GAAGA	5 cut(s)	51, 327, 629, 800, 827
MfeI	CAATTG	1 cut(s)	569
MflI	RGATCY	2 cut(s)	1051, 1072
MhlI	GDGCHC	1 cut(s)	1102
MluCI	AATT	6 cut(s)	38, 414, 435, 569, 851, 983
MlyI	GAGTC	4 cut(s)	5, 157, 683, 1240
MmeI	TCCRAC	2 cut(s)	73, 532
MroI	TCCGGA	1 cut(s)	1136
MroXI	GAANNNNTTC	1 cut(s)	761
MseI	TTAA	2 cut(s)	102, 605
MspI	CCGG	6 cut(s)	170, 191, 906, 1102, 1137, 1275
MspR9I	CCNGG	9 cut(s)	170, 327, 906, 907, 1047, 1102, 1103, 1148, 1276
MunI	CAATTG	1 cut(s)	569
MvaI	CCWGG	3 cut(s)	327, 1047, 1148
MwoI	GCNNNNNNNGC	4 cut(s)	200, 339, 1097, 1207
NciI	CCSGG	6 cut(s)	170, 906, 907, 1102, 1103, 1276
NcoI	CCATGG	1 cut(s)	865
NdeII	GATC	3 cut(s)	16, 1051, 1072
NlaIII	CATG	7 cut(s)	23, 71, 247, 461, 716, 869, 1214
NlaIV	GGNNCC	5 cut(s)	220, 324, 585, 904, 1053
NmuCI	GTSAC	2 cut(s)	130, 965
NspI	RCATGY	1 cut(s)	716
PceI	AGGCCT	1 cut(s)	369
PciSI	GCTCTTC	1 cut(s)	340
PdmI	GAANNNNTTC	1 cut(s)	761
PfeI	GAWTC	5 cut(s)	145, 179, 529, 1140, 1224
PflMI	CCANNNNNTGG	1 cut(s)	458
PkrI	GCNGC	5 cut(s)	175, 814, 931, 994, 1065
PleI	GAGTC	4 cut(s)	5, 157, 682, 1240
PpsI	GAGTC	4 cut(s)	5, 157, 682, 1240
PpuMI	RGGWCCY	2 cut(s)	584, 902
PshAI	GACNNNNGTC	2 cut(s)	55, 900
PsiI	TTATAA	1 cut(s)	707
Psp5II	RGGWCCY	2 cut(s)	584, 902
Psp6I	CCWGG	3 cut(s)	325, 1045, 1146
PspGI	CCWGG	3 cut(s)	325, 1045, 1146
PspN4I	GGNNCC	5 cut(s)	220, 324, 585, 904, 1053
PspPI	GGNCC	2 cut(s)	584, 902
PspPPI	RGGWCCY	2 cut(s)	584, 902
PsuI	RGATCY	2 cut(s)	1051, 1072
RsaI	GTAC	4 cut(s)	594, 666, 794, 883
RsaNI	GTAC	4 cut(s)	593, 665, 793, 882
SapI	GCTCTTC	1 cut(s)	340
SaqAI	TTAA	2 cut(s)	102, 605
SatI	GCNGC	5 cut(s)	174, 813, 930, 993, 1064
Sau3AI	GATC	3 cut(s)	16, 1051, 1072
Sau96I	GGNCC	2 cut(s)	584, 902
ScaI	AGTACT	1 cut(s)	666
SchI	GAGTC	4 cut(s)	5, 157, 683, 1240
ScrFI	CCNGG	9 cut(s)	170, 327, 906, 907, 1047, 1102, 1103, 1148, 1276
SduI	GDGCHC	1 cut(s)	1102
SfcI	CTRYAG	2 cut(s)	363, 1038
SinI	GGWCC	2 cut(s)	584, 902
SmaI	CCCGGG	2 cut(s)	907, 1103
SrfI	GCCCGGGC	1 cut(s)	1103
Sse9I	AATT	6 cut(s)	38, 414, 435, 569, 851, 983
SseBI	AGGCCT	1 cut(s)	369
SsiI	CCGC	3 cut(s)	53, 342, 825
SspI	AATATT	1 cut(s)	207
SspMI	CTAG	3 cut(s)	339, 1077, 1163
StuI	AGGCCT	1 cut(s)	369
StyD4I	CCNGG	9 cut(s)	168, 325, 904, 905, 1045, 1100, 1101, 1146, 1274
StyI	CCWWGG	1 cut(s)	865
TaaI	ACNGT	7 cut(s)	241, 263, 511, 623, 804, 886, 1042
TaiI	ACGT	2 cut(s)	130, 922
TaqI	TCGA	3 cut(s)	504, 652, 957
TaqII	GACCGA	1 cut(s)	927
TasI	AATT	6 cut(s)	38, 414, 435, 569, 851, 983
TatI	WGTACW	3 cut(s)	664, 792, 881
TfiI	GAWTC	5 cut(s)	145, 179, 529, 1140, 1224
Tru1I	TTAA	2 cut(s)	102, 605
Tru9I	TTAA	2 cut(s)	102, 605
TscAI	CASTG	2 cut(s)	403, 516
TseFI	GTSAC	2 cut(s)	130, 965
TseI	GCWGC	5 cut(s)	173, 812, 929, 992, 1063
Tsp45I	GTSAC	2 cut(s)	130, 965
TspDTI	ATGAA	6 cut(s)	51, 264, 281, 821, 864, 963
TspGWI	ACGGA	2 cut(s)	294, 961
TspMI	CCCGGG	2 cut(s)	905, 1101
TspRI	CASTG	2 cut(s)	403, 516
Van91I	CCANNNNNTGG	1 cut(s)	458
VpaK11BI	GGWCC	2 cut(s)	584, 902
XagI	CCTNNNNNAGG	1 cut(s)	897
XapI	RAATTY	4 cut(s)	38, 414, 435, 851
XceI	RCATGY	1 cut(s)	716
XmaI	CCCGGG	2 cut(s)	905, 1101
XmiI	GTMKAC	2 cut(s)	1081, 1156
XmnI	GAANNNNTTC	1 cut(s)	761
XspI	CTAG	3 cut(s)	339, 1077, 1163
ZrmI	AGTACT	1 cut(s)	666

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.