MD13G1002000.v1.1

recombinational repair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003214
Physical Location & Seq
Forward (+)
122143 .. 130387
8245 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1002000.v1.1.491

Sequence Viewer

Length: 3087 bp
ATGTTTGGTCATGCGGATCATCTTTATGACTTCTTTGATTCAACTTGGAGATATTGCTCTTTTTTAAGTGGATGGATTGGCAGTTTAATCTCAAAAAACTGCACGGATGTGATTGGCTTACCTGCAAGGAACTCACTTTCTAAGTTGGTTAAAGAAAGGGAAACTCAATTCTCAACCTTCATGGCTCAAAGGGAATTTATAGATATTAGTTCATCGGATAGTGATTTAGAGATAGAAGAAAGGGAATATGGCAACAGCAGAGTTCTTCCAAGCTCGCTATCTGGACCTGGCTCAAATCCAAGGAGTAGAGATTATTCTGGGCAGTTTAGAAAGGTGCCTTCTCCTAGAAGAGCATATGCTTCTAATGGCAACTCTCCTAATGTTAATCAGCACACACAGGTGAAACAGAATTTTAATCCCAGTTCAAGTGATGATATAAGAACCTCAAATCGACATGCTGCTCGAGCACATAATGATAATGTGGAACGGCCTCAGAGTAATCATTCGCAAAGTTCTAATATTTCTCTTAAGGACTATGAGGCTCAGAAGAGGACCCTTCCACAATCTATGCAGCTATCGGGACCGAGTACTTACACAAGCAATGGCAAGGGCCTTATGAGAGACTATTCTAGTAGGGGCTATGACACCGAATTTAATAGGTCTGAAAGCAGTGGGAGTAGGGGCCAGCCTCCCTCTTTCATGCATGGAAAGTCATTTTCCGCTTCACAGTTTGCGAGTTCAAGTGACCCTGCATACCATTCTGGGACAGGTGACGAAAGGGTTGCTGGCAGTGATGAGAGACTGATATATCAAGCAGCATTAGAGGATCTCAATCAACCAAAAGTTGAAGCTACTTTACCTGATGGTCTTCTGTCGGTCCCTCTTCTACGACATCAGAAAATTGCTCTGGCATGGATGCTTCAAAAGGAAACTAGAAGTCTGCATTGTCTGGGTGGAATCTTAGCAGATGATCAGGGCCTTGGTAAGACCATTTCAATGATTGCCCTTATACAAATGCAGAAGTATTTGGATTCAAAGTCAAAATCTAGAGATTTAGGCAATCAGAAAACTGAGGCTTTAAATTTGGATGACGATGAGGACAATCCCAACGGTGGTTTGGATAAATTGAATAAAACTGAGGCACCTGATCATCTTCGATCAACTCCAGAAGTTAGTACATCTTCACGATCAATCAGAAAGCAGAGGCCAGCAGCGGGTACATTGGTTGTGTGTCCAGCAAGTGTTCTTCGACAGTGGGCTAGGGAGCTGGATGAAAAGGTTGTAGAGGAAGCAAAGCTGCGTGTTATTATTTATCACGGAGGCAGCCGGACGAAGGTTCCTGAAGAACTGGCTAGCTATGATGTGGTTCTCACAACATATGCTATTGTAACCAATGAAGTCCCAAAACAACCTTTAGTTGATGATGATGAACCTGATGAGAAAATGGAGGAAACTTATGGAATACATTCTGATTTTTCAAGTAATAAGAAGAGAAAAAAGGCTTCGATTATTAATAAGAAGGGAAAGAGGGGCAAAAAGGGAATTGATAGTTCCTCTATTGATTGTGGTTCTGGGGCACTTGCAAAAGTGGGTTGGTTTAGGGTGATACTAGATGAAGCTCAGACGATTAAGAATCACAGAACTCAAGTGGCTAGAGCGTGTTGTAGCCTTCGAGCAAAAAGAAGGTGGTGTTTGTCTGGTACTCCTATACAAAATACAATTGATGACTTATACAGTTACTTCAGATTTCTTAGATATGACCCCTATGCTGTATATAAGTCATTTTACAGTACCATTAAGGTTCCAATATCAAGAAACTCAATCCAAGGTTACAAGAAGCTCCAGGCAGTTCTGAGGGCTATAATGTTGCGTCGAACAAAAGGAACATTGATCGATGGGCAACCTATAATTGAATTACCACCAAAAACAATAAACTTGAGTAAAGTGGAGTTCTCGCCTGAGGAGCGGGCCTTTTATACCAAGCTAGAAGCCGATTCACGCACTAAGTTCAAGGCATATGCCGCTGCTGGCACAGTAAATCAAAATTATGCAAATATTCTTTTGATGCTTTTGCGCCTCCGGCAAGCTTGTGACCACCCTCAGCTTGTTAAAGCGTATGACTCAGACTGTGTTGGGAAAGATTCTGTGCAAATGGCAAGGCGACTCCCAAAGGATATGATATCCCATCTTTTGCATCTTCTGGAAACTTCATTGGCTATTTGTCGTGTATGCAAAGATCCACCTGAAGACCCTGTTGTTACTATGTGTGGCCATGTATTCTGTTATCAGTGTGTTTCGGAATATTTGACGGGTGATGACAATACATGTCCTGCTGCTGAATGTAAAGAACAAGTTGGTTCTGATGTTGTTTTCTCCAAATCCACTCTCATAAGTTGCTTCTCCAATAATCTTGATGGTACTCGGACGAATTCTGAGTTGGGCGAGAAATCAATAGTGTTACAGAATGAGTATAGTTCATCTAAAGTCAGAGCTATTATTGAGATTTTGCTTTCACATTTGGAACATAACTGTGCTGGAAGCAATGGAGACCCTGCTTTTGGAACCGAAATCACAGATAGTAGATATTCTGGCGTTTCAAGCTCACCAAATAGTGGACCAATAAAAACGATTATTTTTTCCCAGTGGACTGGCATGTTGGATTTAGTTGAAACATCATTGAACGAGTATTGTATTCAGTACAGAAGGCTTGATGGGACAATGAGTCTGGCATCAAGAGACAGGGGTGTCAAAGATTTTAACACTGATCCTGAGATCACTGTTATGCTCATGTCACTAAAGGCTGGAAACCTTGGTCTAAACATGGTTGCAGCATGTCATGTTATCCTTTTGGACCTGTGGTGGAATCCAACCACTGAAGATCAAGCTATTGATCGGGCACATAGAATTGGACAGACCCGACCTGTTACTGTAACACGTCTCACGATTAAGGATACAGTGGAGGACAGGATATTAGCTTTACAGGAAGAAAAGAGAAAAATGGTGGCATCTGCTTTTGGTGAAGATCATGGTGGGGGCTCTGCTACACGCCTGACTGTTGAGGATCTGAGATACCTATTTATGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000278 GO:0000724 GO:0000725 GO:0000785 GO:0000790 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006304 GO:0006305 GO:0006306 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007154 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009059 GO:0009292 GO:0009294 GO:0009605 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0009991 GO:0010468 GO:0010605 GO:0010629 GO:0016043 GO:0016070 GO:0016458 GO:0016462 GO:0016567 GO:0016740 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019538 GO:0019787 GO:0022402 GO:0030162 GO:0031047 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031668 GO:0031974 GO:0031981 GO:0032259 GO:0032268 GO:0032269 GO:0032392 GO:0032434 GO:0032435 GO:0032446 GO:0032508 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042176 GO:0042177 GO:0042262 GO:0042623 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0044764 GO:0044786 GO:0045003 GO:0045005 GO:0045861 GO:0046483 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051276 GO:0051704 GO:0051716 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070035 GO:0070647 GO:0071103 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0140083 GO:0140096 GO:0140097 GO:1901360 GO:1901564 GO:1901576 GO:1901799 GO:1902298 GO:1902969 GO:1903047 GO:1903050 GO:1903051 GO:1903362 GO:1903363 GO:1990505 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
ko04918 map04918
Pfam Domains
Protein Families
Helicase_C SNF2_N zf-C3HC4 zf-C3HC4_2 zf-C3HC4_3

Protein Analysis

1029

Amino Acids

114.6

Weight (kDa)

8.07

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 298 - 702 1.6e-68 SNF2-related domain
zf-C3HC4_3 PF13920 740 - 784 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 741 - 777 7e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 741 - 777 2.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 741 - 777 2.3e-06 RING-type zinc-finger
Helicase_C PF00271 875 - 970 7.9e-19 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2744
Acc36I ACCTGC 1 cut(s) 130
AccB1I GGYRCC 2 cut(s) 334, 1141
AccB7I CCANNNNNTGG 1 cut(s) 2612
AccBSI CCGCTC 1 cut(s) 1966
AciI CCGC 5 cut(s) 14, 720, 1214, 1966, 2022
AclWI GGATC 5 cut(s) 24, 834, 2231, 2759, 3069
AcoI YGGCCR 1 cut(s) 2269
AcsI RAATTY 5 cut(s) 194, 409, 650, 1081, 2428
AcuI CTGAAG 4 cut(s) 1362, 1726, 2265, 2895
AfaI GTAC 7 cut(s) 589, 1177, 1219, 1702, 1792, 2419, 2699
AfiI CCNNNNNNNGG 6 cut(s) 1112, 1214, 1333, 2558, 2612, 3079
AflII CTTAAG 1 cut(s) 527
AflIII ACRYGT 2 cut(s) 2324, 2933
AhdI GACNNNNNGTC 1 cut(s) 2721
AjiI CACGTC 1 cut(s) 2936
AjnI CCWGG 2 cut(s) 286, 1842
AjuI GAANNNNNNNTTGG 2 cut(s) 2420, 2452
AleI CACNNNNGTG 2 cut(s) 107, 398
Alw21I GWGCWC 1 cut(s) 469
Alw26I GTCTC 5 cut(s) 615, 793, 2541, 2730, 2942
AlwI GGATC 5 cut(s) 24, 834, 2231, 2759, 3069
Ama87I CYCGRG 1 cut(s) 462
AoxI GGCC 7 cut(s) 488, 610, 682, 976, 1205, 1968, 2269
ApeKI GCWGC 9 cut(s) 458, 571, 815, 1211, 1297, 1323, 2024, 2333, 2828
ApoI RAATTY 5 cut(s) 194, 409, 650, 1081, 2428
ArsI GACNNNNNNTTYG 2 cut(s) 2540, 2572
AseI ATTAAT 1 cut(s) 1512
Asp700I GAANNNNTTC 1 cut(s) 1465
AspLEI GCGC 1 cut(s) 2076
AsuHPI GGTGA 6 cut(s) 412, 782, 1615, 2324, 2595, 3029
AsuNHI GCTAGC 1 cut(s) 1352
AvaI CYCGRG 1 cut(s) 462
AvaII GGWCC 6 cut(s) 284, 552, 581, 877, 2615, 2851
AxyI CCTNAGG 1 cut(s) 1959
BaeGI GKGCMC 2 cut(s) 1579, 2899
BaeI ACNNNNGTAYC 2 cut(s) 1209, 1242
BalI TGGCCA 1 cut(s) 2271
BanI GGYRCC 2 cut(s) 334, 1141
BanII GRGCYC 1 cut(s) 3038
BarI GAAGNNNNNNTAC 2 cut(s) 840, 872
BbsI GAAGAC 2 cut(s) 860, 2253
Bbv12I GWGCWC 1 cut(s) 469
BbvCI CCTCAGC 1 cut(s) 2100
BbvI GCAGC 9 cut(s) 445, 583, 827, 1223, 1284, 1335, 2011, 2320, 2840
BccI CCATC 6 cut(s) 66, 857, 1889, 2193, 2408, 2705
BceAI ACGGC 1 cut(s) 503
BcgI CGANNNNNNTGC 2 cut(s) 764, 798
BciT130I CCWGG 2 cut(s) 288, 1844
BciVI GTATCC 1 cut(s) 2944
BclI TGATCA 2 cut(s) 970, 1147
BcoDI GTCTC 5 cut(s) 615, 793, 2541, 2730, 2942
BfaI CTAG 9 cut(s) 345, 630, 933, 1047, 1260, 1353, 1610, 1653, 1985
BfrI CTTAAG 1 cut(s) 527
BfuAI ACCTGC 1 cut(s) 130
BfuI GTATCC 1 cut(s) 2944
BmcAI AGTACT 1 cut(s) 589
Bme1390I CCNGG 2 cut(s) 288, 1844
Bme18I GGWCC 6 cut(s) 284, 552, 581, 877, 2615, 2851
BmeRI GACNNNNNGTC 1 cut(s) 2721
BmeT110I CYCGRG 1 cut(s) 462
BmgBI CACGTC 1 cut(s) 2936
BmiI GGNNCC 9 cut(s) 336, 554, 582, 683, 879, 1143, 1338, 1803, 2563
BmrFI CCNGG 2 cut(s) 288, 1844
BmrI ACTGGG 2 cut(s) 414, 2635
BmsI GCATC 5 cut(s) 906, 2055, 2203, 2738, 3014
BmtI GCTAGC 1 cut(s) 1356
BmuI ACTGGG 2 cut(s) 414, 2635
BpiI GAAGAC 2 cut(s) 860, 2253
BpmI CTGGAG 2 cut(s) 1149, 1826
Bpu10I CCTNAGC 1 cut(s) 2100
BpuEI CTTGAG 2 cut(s) 1629, 1957
Bsa29I ATCGAT 1 cut(s) 1893
BsaBI GATNNNNATC 1 cut(s) 831
BsaI GGTCTC 1 cut(s) 2541
BsaJI CCNNGG 4 cut(s) 299, 979, 1825, 2809
Bsc4I CCNNNNNNNGG 6 cut(s) 1112, 1214, 1333, 2558, 2612, 3079
Bse1I ACTGG 4 cut(s) 420, 1353, 2641, 2653
Bse21I CCTNAGG 1 cut(s) 1959
Bse3DI GCAATG 2 cut(s) 607, 2548
Bse8I GATNNNNATC 1 cut(s) 831
BseBI CCWGG 2 cut(s) 288, 1844
BseCI ATCGAT 1 cut(s) 1893
BseDI CCNNGG 4 cut(s) 299, 979, 1825, 2809
BseGI GGATG 5 cut(s) 77, 112, 921, 1093, 1276
BseJI GATNNNNATC 1 cut(s) 831
BseLI CCNNNNNNNGG 6 cut(s) 1112, 1214, 1333, 2558, 2612, 3079
BseMI GCAATG 2 cut(s) 607, 2548
BseNI ACTGG 4 cut(s) 420, 1353, 2641, 2653
BseRI GAGGAG 1 cut(s) 1976
BseSI GKGCMC 2 cut(s) 1579, 2899
BseXI GCAGC 9 cut(s) 445, 583, 827, 1223, 1284, 1335, 2011, 2320, 2840
BsgI GTGCAG 1 cut(s) 85
BshFI GGCC 7 cut(s) 490, 612, 684, 978, 1207, 1970, 2271
BshNI GGYRCC 2 cut(s) 334, 1141
BshVI ATCGAT 1 cut(s) 1893
BsiHKAI GWGCWC 1 cut(s) 469
BsiHKCI CYCGRG 1 cut(s) 462
BsiSI CCGG 2 cut(s) 1327, 2080
BslFI GGGAC 5 cut(s) 594, 778, 863, 1385, 2728
BslI CCNNNNNNNGG 6 cut(s) 1112, 1214, 1333, 2558, 2612, 3079
BsmAI GTCTC 5 cut(s) 615, 793, 2541, 2730, 2942
BsmBI CGTCTC 1 cut(s) 2942
BsmFI GGGAC 5 cut(s) 594, 778, 863, 1385, 2728
BsnI GGCC 7 cut(s) 490, 612, 684, 978, 1207, 1970, 2271
Bso31I GGTCTC 1 cut(s) 2541
BsoBI CYCGRG 1 cut(s) 462
Bsp1286I GDGCHC 4 cut(s) 469, 1579, 2899, 3038
BspACI CCGC 5 cut(s) 14, 720, 1214, 1966, 2022
BspANI GGCC 7 cut(s) 490, 612, 684, 978, 1207, 1970, 2271
BspDI ATCGAT 1 cut(s) 1893
BspLI GGNNCC 9 cut(s) 336, 554, 582, 683, 879, 1143, 1338, 1803, 2563
BspMI ACCTGC 1 cut(s) 130
BspOI GCTAGC 1 cut(s) 1356
BspPI GGATC 5 cut(s) 24, 834, 2231, 2759, 3069
BspQI GCTCTTC 1 cut(s) 343
BspT107I GGYRCC 2 cut(s) 334, 1141
BspTI CTTAAG 1 cut(s) 527
BspTNI GGTCTC 1 cut(s) 2541
BsrBI CCGCTC 1 cut(s) 1966
BsrDI GCAATG 2 cut(s) 607, 2548
BsrI ACTGG 4 cut(s) 420, 1353, 2641, 2653
BssECI CCNNGG 4 cut(s) 299, 979, 1825, 2809
BssT1I CCWWGG 4 cut(s) 299, 979, 1825, 2809
Bst2UI CCWGG 2 cut(s) 288, 1844
Bst6I CTCTTC 4 cut(s) 343, 542, 888, 1484
BstAFI CTTAAG 1 cut(s) 527
BstC8I GCNNGC 8 cut(s) 275, 686, 787, 1209, 1354, 1968, 2029, 2085
BstF5I GGATG 5 cut(s) 77, 112, 921, 1093, 1276
BstHHI GCGC 1 cut(s) 2076
BstMAI GTCTC 5 cut(s) 615, 793, 2541, 2730, 2942
BstMWI GCNNNNNNNGC 5 cut(s) 464, 1963, 2021, 2080, 2598
BstNI CCWGG 2 cut(s) 288, 1844
BstNSI RCATGY 4 cut(s) 458, 2328, 2656, 2835
BstSCI CCNGG 2 cut(s) 286, 1842
BstSLI GKGCMC 2 cut(s) 1579, 2899
BstV1I GCAGC 9 cut(s) 445, 583, 827, 1223, 1284, 1335, 2011, 2320, 2840
BstV2I GAAGAC 2 cut(s) 860, 2253
BstX2I RGATCY 3 cut(s) 826, 2236, 3061
BstXI CCANNNNNNTGG 1 cut(s) 2648
BstYI RGATCY 3 cut(s) 826, 2236, 3061
Bsu15I ATCGAT 1 cut(s) 1893
Bsu36I CCTNAGG 1 cut(s) 1959
BsuI GTATCC 1 cut(s) 2944
BsuRI GGCC 7 cut(s) 490, 612, 684, 978, 1207, 1970, 2271
BsuTUI ATCGAT 1 cut(s) 1893
BtrI CACGTC 1 cut(s) 2936
BtsCI GGATG 5 cut(s) 77, 112, 921, 1093, 1276
BtsI GCAGTG 2 cut(s) 676, 796
BtsIMutI CAGTG 9 cut(s) 676, 796, 1259, 2294, 2648, 2760, 2775, 2871, 2961
BveI ACCTGC 1 cut(s) 130
Cac8I GCNNGC 8 cut(s) 275, 686, 787, 1209, 1354, 1968, 2029, 2085
CfoI GCGC 1 cut(s) 2076
ClaI ATCGAT 1 cut(s) 1893
CseI GACGC 1 cut(s) 1859
Csp6I GTAC 7 cut(s) 588, 1176, 1218, 1701, 1791, 2418, 2698
CviQI GTAC 7 cut(s) 588, 1176, 1218, 1701, 1791, 2418, 2698
DraI TTTAAA 1 cut(s) 1080
DrdI GACNNNNNNGTC 1 cut(s) 2744
DriI GACNNNNNGTC 1 cut(s) 2721
DseDI GACNNNNNNGTC 1 cut(s) 2744
EaeI YGGCCR 1 cut(s) 2269
Eam1104I CTCTTC 4 cut(s) 343, 542, 888, 1484
Eam1105I GACNNNNNGTC 1 cut(s) 2721
EarI CTCTTC 4 cut(s) 343, 542, 888, 1484
Eco130I CCWWGG 4 cut(s) 299, 979, 1825, 2809
Eco24I GRGCYC 1 cut(s) 3038
Eco31I GGTCTC 1 cut(s) 2541
Eco32I GATATC 1 cut(s) 2181
Eco47I GGWCC 6 cut(s) 284, 552, 581, 877, 2615, 2851
Eco57I CTGAAG 4 cut(s) 1362, 1726, 2265, 2895
Eco81I CCTNAGG 1 cut(s) 1959
Eco88I CYCGRG 1 cut(s) 462
EcoO109I RGGNCCY 3 cut(s) 552, 610, 976
EcoRI GAATTC 1 cut(s) 2428
EcoRII CCWGG 2 cut(s) 286, 1842
EcoRV GATATC 1 cut(s) 2181
EcoT14I CCWWGG 4 cut(s) 299, 979, 1825, 2809
EcoT22I ATGCAT 1 cut(s) 705
EcoT38I GRGCYC 1 cut(s) 3038
ErhI CCWWGG 4 cut(s) 299, 979, 1825, 2809
Esp3I CGTCTC 1 cut(s) 2942
FaqI GGGAC 5 cut(s) 594, 778, 863, 1385, 2728
FauI CCCGC 2 cut(s) 1207, 1959
FauNDI CATATG 3 cut(s) 355, 1378, 2017
FbaI TGATCA 2 cut(s) 970, 1147
FokI GGATG 5 cut(s) 84, 119, 928, 1100, 1283
FriOI GRGCYC 1 cut(s) 3038
FspBI CTAG 9 cut(s) 345, 630, 933, 1047, 1260, 1353, 1610, 1653, 1985
GlaI GCGC 1 cut(s) 2075
GsuI CTGGAG 2 cut(s) 1149, 1826
HaeIII GGCC 7 cut(s) 490, 612, 684, 978, 1207, 1970, 2271
HapII CCGG 2 cut(s) 1327, 2080
HgaI GACGC 1 cut(s) 1859
HhaI GCGC 1 cut(s) 2076
Hin6I GCGC 1 cut(s) 2074
HinP1I GCGC 1 cut(s) 2074
HindIII AAGCTT 1 cut(s) 2085
HpaII CCGG 2 cut(s) 1327, 2080
HphI GGTGA 6 cut(s) 412, 782, 1615, 2324, 2595, 3029
Hpy166II GTNNAC 2 cut(s) 2615, 2646
Hpy8I GTNNAC 2 cut(s) 2615, 2646
Hpy99I CGWCG 1 cut(s) 1875
HpyAV CCTTC 8 cut(s) 187, 348, 566, 1327, 1513, 1677, 1679, 2697
HpyCH4IV ACGT 1 cut(s) 2935
HpyF10VI GCNNNNNNNGC 5 cut(s) 464, 1963, 2021, 2080, 2598
HpySE526I ACGT 1 cut(s) 2935
HspAI GCGC 1 cut(s) 2074
Ksp22I TGATCA 2 cut(s) 970, 1147
LguI GCTCTTC 1 cut(s) 343
LmnI GCTCC 3 cut(s) 1264, 1845, 1963
Lsp1109I GCAGC 9 cut(s) 445, 583, 827, 1223, 1284, 1335, 2011, 2320, 2840
LweI GCATC 5 cut(s) 906, 2055, 2203, 2738, 3014
MaeI CTAG 9 cut(s) 345, 630, 933, 1047, 1260, 1353, 1610, 1653, 1985
MaeII ACGT 1 cut(s) 2935
MbiI CCGCTC 1 cut(s) 1966
MfeI CAATTG 1 cut(s) 1719
MflI RGATCY 3 cut(s) 826, 2236, 3061
MhlI GDGCHC 4 cut(s) 469, 1579, 2899, 3038
MlsI TGGCCA 1 cut(s) 2271
MluNI TGGCCA 1 cut(s) 2271
MlyI GAGTC 3 cut(s) 2114, 2157, 2731
MmeI TCCRAC 2 cut(s) 2637, 2891
Mox20I TGGCCA 1 cut(s) 2271
Mph1103I ATGCAT 1 cut(s) 705
MroXI GAANNNNTTC 1 cut(s) 1465
MscI TGGCCA 1 cut(s) 2271
MslI CAYNNNNRTG 6 cut(s) 24, 107, 398, 995, 2529, 2780
Msp20I TGGCCA 1 cut(s) 2271
MspA1I CMGCKG 2 cut(s) 1214, 2024
MspCI CTTAAG 1 cut(s) 527
MspI CCGG 2 cut(s) 1327, 2080
MspR9I CCNGG 2 cut(s) 288, 1844
MunI CAATTG 1 cut(s) 1719
MvaI CCWGG 2 cut(s) 288, 1844
MwoI GCNNNNNNNGC 5 cut(s) 464, 1963, 2021, 2080, 2598
NdeI CATATG 3 cut(s) 355, 1378, 2017
NheI GCTAGC 1 cut(s) 1352
NlaIV GGNNCC 9 cut(s) 336, 554, 582, 683, 879, 1143, 1338, 1803, 2563
NmuCI GTSAC 4 cut(s) 743, 770, 2090, 2790
NsiI ATGCAT 1 cut(s) 705
NspI RCATGY 4 cut(s) 458, 2328, 2656, 2835
OliI CACNNNNGTG 2 cut(s) 107, 398
PaeR7I CTCGAG 1 cut(s) 462
PciI ACATGT 1 cut(s) 2324
PciSI GCTCTTC 1 cut(s) 343
PdmI GAANNNNTTC 1 cut(s) 1465
PfeI GAWTC 7 cut(s) 38, 957, 1031, 1633, 1994, 2141, 2863
PflMI CCANNNNNTGG 1 cut(s) 2612
PleI GAGTC 3 cut(s) 2114, 2157, 2730
PpsI GAGTC 3 cut(s) 2114, 2157, 2730
PpuMI RGGWCCY 1 cut(s) 552
PscI ACATGT 1 cut(s) 2324
PshBI ATTAAT 1 cut(s) 1512
Psp5II RGGWCCY 1 cut(s) 552
Psp6I CCWGG 2 cut(s) 286, 1842
PspGI CCWGG 2 cut(s) 286, 1842
PspN4I GGNNCC 9 cut(s) 336, 554, 582, 683, 879, 1143, 1338, 1803, 2563
PspPPI RGGWCCY 1 cut(s) 552
PspXI VCTCGAGB 1 cut(s) 462
PsuI RGATCY 3 cut(s) 826, 2236, 3061
RsaI GTAC 7 cut(s) 589, 1177, 1219, 1702, 1792, 2419, 2699
RsaNI GTAC 7 cut(s) 588, 1176, 1218, 1701, 1791, 2418, 2698
RseI CAYNNNNRTG 6 cut(s) 24, 107, 398, 995, 2529, 2780
SapI GCTCTTC 1 cut(s) 343
ScaI AGTACT 1 cut(s) 589
SchI GAGTC 3 cut(s) 2114, 2157, 2731
ScrFI CCNGG 2 cut(s) 288, 1844
SduI GDGCHC 4 cut(s) 469, 1579, 2899, 3038
SfaNI GCATC 5 cut(s) 906, 2055, 2203, 2738, 3014
Sfr274I CTCGAG 1 cut(s) 462
SinI GGWCC 6 cut(s) 284, 552, 581, 877, 2615, 2851
SlaI CTCGAG 1 cut(s) 462
SmiMI CAYNNNNRTG 6 cut(s) 24, 107, 398, 995, 2529, 2780
SmlI CTYRAG 4 cut(s) 462, 527, 1644, 1936
SmoI CTYRAG 4 cut(s) 462, 527, 1644, 1936
SsiI CCGC 5 cut(s) 14, 720, 1214, 1966, 2022
SspI AATATT 3 cut(s) 520, 2056, 2303
SspMI CTAG 9 cut(s) 345, 630, 933, 1047, 1260, 1353, 1610, 1653, 1985
StyD4I CCNGG 2 cut(s) 286, 1842
StyI CCWWGG 4 cut(s) 299, 979, 1825, 2809
TaiI ACGT 1 cut(s) 2938
TaqI TCGA 8 cut(s) 451, 463, 1156, 1249, 1505, 1672, 1873, 1893
TaqII GACCGA 2 cut(s) 598, 865
TatI WGTACW 3 cut(s) 587, 1175, 2697
TauI GCSGC 1 cut(s) 2024
TfiI GAWTC 7 cut(s) 38, 957, 1031, 1633, 1994, 2141, 2863
TscAI CASTG 9 cut(s) 676, 796, 1259, 2294, 2648, 2767, 2782, 2878, 2961
TseFI GTSAC 4 cut(s) 743, 770, 2090, 2790
TseI GCWGC 9 cut(s) 458, 571, 815, 1211, 1297, 1323, 2024, 2333, 2828
Tsp45I GTSAC 4 cut(s) 743, 770, 2090, 2790
TspDTI ATGAA 9 cut(s) 169, 201, 688, 1287, 1410, 1443, 1629, 2199, 2466
TspGWI ACGGA 2 cut(s) 119, 1332
TspRI CASTG 9 cut(s) 676, 796, 1259, 2294, 2648, 2767, 2782, 2878, 2961
Van91I CCANNNNNTGG 1 cut(s) 2612
Vha464I CTTAAG 1 cut(s) 527
VpaK11BI GGWCC 6 cut(s) 284, 552, 581, 877, 2615, 2851
VspI ATTAAT 1 cut(s) 1512
XapI RAATTY 5 cut(s) 194, 409, 650, 1081, 2428
XbaI TCTAGA 1 cut(s) 1046
XceI RCATGY 4 cut(s) 458, 2328, 2656, 2835
XcmI CCANNNNNNNNNTGG 1 cut(s) 1114
XhoI CTCGAG 1 cut(s) 462
XmnI GAANNNNTTC 1 cut(s) 1465
XspI CTAG 9 cut(s) 345, 630, 933, 1047, 1260, 1353, 1610, 1653, 1985
ZrmI AGTACT 1 cut(s) 589
Zsp2I ATGCAT 1 cut(s) 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.