pycom16g00060

recombinational repair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003214
Physical Location & Seq
Forward (+)
30384 .. 36588
6205 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g00060.2

Sequence Viewer

Length: 3087 bp
ATGTTTGGTCATGCGGATCATCTTCATGATTTCTTTGATTCAACTTGGAGACATTGCTCTTTTTTAAGTGGATGGATTGGCAGTTTAATCTCAAAATACTGCACGGACGTGATTGAGTTACCTGCAAGGAATTCACTTTCTAAGTTGGCTGAAGAAAGGGAAACTCAATTGTCAACCTTCATGGCTCAAAGGGAATTTATAGATATTAGTTCATCGGATAGTGAATTAGAGATAGAAGAAAGGGAATATGGCAACAGCAGAGTTCTTCCAAGCTCGCTGTCTGGACCTGGCTCAAATCCAAGGAGTAGAGATTATTCTGGGCAGTCTAGAAACGTGCCTTCTCCTAGAAGAGCATATGCGTCTAATGGCAACTCTCCTAATATTAATCCGCACACACAGGTGAAACAGAATTTTAATCCCAGTTCAAGTGATGATATAAGAACCTCAAATCGACATGCTGCTCGAGCACATAATGGTTATGCAGAACGGCCTCAAAGTAATCATTCACAAATTTATAATATTTCTGTTAAGGACTATGAAGCTCATAAGAGGACCCTTCCGCCGTCTATGCAGCTATCGGGACCAAGTACTTATGCAACTAATGGCAAGGGCCTTATGAGAGACTATTCAAGTAGGGGCTATGACAATGAATTTAATAGGTCTGAGAGCAGTGGGAGTAGGGGCCAGCCTCCCTCTTTCATGCATGGAAAGTCATTTTCCACTTCACAGTTTGCTAGTTCAAGTGACCCTGCATACCATGGTGGGACAGGTGACGATAGGGTGGCTGGCAGTGATGAGAGACTGATATATCAAGCAGCATTAGAGGATCTCAATCAACCAAAAGTTGAAGCTACTTTACCTGATGGTCTTCTGTCTGTCCCTCTTCTACGACATCAGAAAATTGCTCTGGCATGGATGCTTCAAAAGGAAACTAGAAGTCTGCATTGTCTAGGTGGAATCTTAGCAGATGATCAGGGCCTTGGTAAGACCATTTCAATGATTTCCCTTATACAAATGCAGAAGTATTTGGATTCAAAATCAAAATCTAGAGAGTTAGGCAATCAGAAAACTGAAGCTTTAAATTTGGATGATGATGAGGACAATCCCAGCGGTGGTTTGGATAAATTGAATAAAACTGAGCAACCTGATCATCTTCGATCAATTCCAGAAGTTAGTACATCTTCACGGTCAATCAGAAAGCAGAGGCCAGCAGCGGGTACATTGGTTGTGTGTCCAGCAAGTGTTCTTCGACAGTGGGCTAGGGAGCTGGATGAAAAGGTTGCAGAGGAAGCAAAGCTGCGTGTCCTTATTTATCATGGAGGCAGCCGGACGAAGAATCCTGAAGAACTGGCTAGCTATGACGTGATTCTCACAACATATGCTATTGTAACCAATGAAGTCCCAAAACAACCTTTAGTTGATGACGATGAACCTGATGAGAAAAATGGGGAAACTTATGGAGTACATTCCGATTTTTCAAGTGATAAGAAGAGAAAAAAGGCTTCGGTTGTTAATAAGAAGAGAAAGAGGGGCAAAAAAGGAATTGACAGTTCATCTATTGATTGCGGTTCTGGTGCACTTGCAAAAGTGGGTTGGTTTAGGGTGATACTAGATGAAGCTCAGACAATTAAGAATCACAGAACTCAAGTGGCTAGAGCGTGTTGTAGCCTTCGAGCAAAAAGAAGGTGGTGTTTGTCTGGTACTCCTATACAAAATACAATTGATGATTTATACAGTTACTTTAGATTTCTTAGATATGACCCCTATGCTGTATATAAGTCATTTTACAGTACCATTAAGGTTCCAATATCGAGAAATTCGATCCAAGGTTATAAGAAGCTCCAGGCAGTTCTGAGGGCTATAATGTTGCGCCGAACAAAAGGAACATTGATCGATGGGCAACCTATAATTGAGTTACCACCAAAAACAATAAACTTGAGTAAAGTGGAGTTCTCATCTGAGGAGCGTGCTTTCTATACCAAGCTAGAAGCTGATTCACGCACTAAGTTCAAGGCATATGCCGCTGCTGGCACAGTGAATCAAAACTATGCAAATATTCTTTTGATGCTTTTGCGCCTCCGGCAAGCTTGTGACCACCCTCAGCTTGTTAAAGCATATGACTCTGACTGTGTCGGGAAAGATTCTGTGCAAATGGCAAGGCGACTCCCAAAGGATATGATTTCCCATCTTTTGCATCTTCTGGAAACTTCATTGGCCATTTGTCGTGTATGCAAAGATCCACCTGAAGGCCCTGTTGTTACTATGTGTGGCCATGTATTCTGCTATCAGTGTGTTTCAGAACACTTGACGGGTGATGACAGTACATGCCCTGATGCTGGATGTAAAGAACAAGTTGGTTCTGATGTTGTTTTCTCCAAATCCACTCTCATAAGTTGCCTCTCCAATAATCTTGATGGTACTCCGATGAATTCTGAGTTGGGCGAGAAATCAATTGTGTTACAGAATGAGTATAGTTCATCTAAAGTCAGAGCTATTATTGAGATTTTGCAGTCACATTTGGAACATAACTCTACTGGAAGCAATGGAGACCCTGCTTTTGGAACCGAAATCACAGATAGTATATATTCTGGCTTTTCAAGCTCACCAAATAATGGACCAATCAAAACGATTATTTTCTCCCAGTGGACTGGCATGTTGGATTTAGTTGAGGCATCATTGAACGAGTATTGTATACAGTACAGAAGGCTTGATGGTACAATGAGTCTGGCATCAAGAGACAGGGCTGTGAAAGATTTTAACACTGATCCTGAGATCATTGTTATGCTCATGTCACTAAAGGCTGGAAACCTTGGTCTCAACATGGTTGCTGCATGTCATGTTATCCTTTTGGACCTGTGGTGGAATCCAACTACTGAAGATCAAGCTATTGATCGGGCACATAGAATTGGACAGACCCGACCTGTTACTGTAACACGTCTCACAATTAAGGATACAGTGGAGGACAGGATATTAGCTTTACAGGAAGAAAAGAGAAAAATGGTGGCATCTGCTTTTGGTGAAGATCATGGTGGGGGCTCTGCTACACGTCTGACTGTCGAGGATCTGAGGTACCTATTTATGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000278 GO:0000724 GO:0000725 GO:0000785 GO:0000790 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006304 GO:0006305 GO:0006306 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007154 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009059 GO:0009292 GO:0009294 GO:0009605 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0009991 GO:0010468 GO:0010605 GO:0010629 GO:0016043 GO:0016070 GO:0016458 GO:0016462 GO:0016567 GO:0016740 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019538 GO:0019787 GO:0022402 GO:0030162 GO:0031047 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031668 GO:0031974 GO:0031981 GO:0032259 GO:0032268 GO:0032269 GO:0032392 GO:0032434 GO:0032435 GO:0032446 GO:0032508 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042176 GO:0042177 GO:0042262 GO:0042623 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0044764 GO:0044786 GO:0045003 GO:0045005 GO:0045861 GO:0046483 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051276 GO:0051704 GO:0051716 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070035 GO:0070647 GO:0071103 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0140083 GO:0140096 GO:0140097 GO:1901360 GO:1901564 GO:1901576 GO:1901799 GO:1902298 GO:1902969 GO:1903047 GO:1903050 GO:1903051 GO:1903362 GO:1903363 GO:1990505 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
ko04918 map04918
Pfam Domains
Protein Families
Helicase_C SNF2_N zf-C3HC4 zf-C3HC4_2 zf-C3HC4_3

Protein Analysis

1029

Amino Acids

114.43

Weight (kDa)

7.55

Isoelectric Point (pI)

51.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 516, 1833
Acc36I ACCTGC 1 cut(s) 130
Acc65I GGTACC 1 cut(s) 3069
AccB1I GGYRCC 1 cut(s) 3069
AccB7I CCANNNNNTGG 1 cut(s) 2612
AccI GTMKAC 1 cut(s) 2692
AciI CCGC 7 cut(s) 14, 389, 560, 1110, 1214, 1566, 2022
AclWI GGATC 6 cut(s) 24, 834, 1816, 2231, 2759, 3069
AcoI YGGCCR 2 cut(s) 2214, 2269
AcsI RAATTY 8 cut(s) 130, 194, 409, 510, 650, 1081, 1816, 2428
AcuI CTGAAG 5 cut(s) 171, 1092, 1362, 2265, 2895
AfiI CCNNNNNNNGG 7 cut(s) 1112, 1214, 2246, 2336, 2558, 2612, 3079
AflIII ACRYGT 2 cut(s) 2933, 3044
AjiI CACGTC 4 cut(s) 109, 1363, 2936, 3047
AjnI CCWGG 2 cut(s) 286, 1842
AjuI GAANNNNNNNTTGG 2 cut(s) 2420, 2452
AleI CACNNNNGTG 2 cut(s) 107, 398
Alw21I GWGCWC 2 cut(s) 469, 1579
Alw26I GTCTC 7 cut(s) 43, 615, 793, 2541, 2730, 2819, 2942
Alw44I GTGCAC 1 cut(s) 1575
AlwI GGATC 6 cut(s) 24, 834, 1816, 2231, 2759, 3069
Ama87I CYCGRG 1 cut(s) 462
AoxI GGCC 8 cut(s) 488, 610, 682, 976, 1205, 2214, 2248, 2269
ApaLI GTGCAC 1 cut(s) 1575
ApeKI GCWGC 8 cut(s) 458, 571, 815, 1211, 1297, 1323, 2024, 2828
ApoI RAATTY 8 cut(s) 130, 194, 409, 510, 650, 1081, 1816, 2428
ArsI GACNNNNNNTTYG 2 cut(s) 2540, 2572
AseI ATTAAT 1 cut(s) 384
Asp718I GGTACC 1 cut(s) 3069
AspLEI GCGC 2 cut(s) 1872, 2076
AspS9I GGNCC 9 cut(s) 284, 552, 581, 610, 682, 976, 2249, 2615, 2851
AsuHPI GGTGA 6 cut(s) 412, 782, 1615, 2324, 2595, 3029
AsuNHI GCTAGC 1 cut(s) 1352
AvaI CYCGRG 1 cut(s) 462
AvaII GGWCC 5 cut(s) 284, 552, 581, 2615, 2851
BaeGI GKGCMC 2 cut(s) 1579, 2899
BaeI ACNNNNGTAYC 2 cut(s) 1209, 1242
BalI TGGCCA 2 cut(s) 2216, 2271
BanI GGYRCC 1 cut(s) 3069
BanII GRGCYC 1 cut(s) 3038
BarI GAAGNNNNNNTAC 2 cut(s) 840, 872
BbsI GAAGAC 1 cut(s) 860
Bbv12I GWGCWC 2 cut(s) 469, 1579
BbvCI CCTCAGC 1 cut(s) 2100
BbvI GCAGC 8 cut(s) 445, 583, 827, 1223, 1284, 1335, 2011, 2815
BccI CCATC 6 cut(s) 66, 857, 1889, 2193, 2408, 2705
BceAI ACGGC 2 cut(s) 503, 547
BciT130I CCWGG 2 cut(s) 288, 1844
BciVI GTATCC 1 cut(s) 2944
BclI TGATCA 2 cut(s) 970, 1147
BcoDI GTCTC 7 cut(s) 43, 615, 793, 2541, 2730, 2819, 2942
BfuAI ACCTGC 1 cut(s) 130
BfuI GTATCC 1 cut(s) 2944
BisI GCNGC 9 cut(s) 459, 572, 816, 1212, 1298, 1324, 2022, 2025, 2829
BlsI GCNGC 9 cut(s) 460, 573, 817, 1213, 1299, 1325, 2023, 2026, 2830
BmcAI AGTACT 1 cut(s) 589
Bme1390I CCNGG 2 cut(s) 288, 1844
Bme18I GGWCC 5 cut(s) 284, 552, 581, 2615, 2851
BmeT110I CYCGRG 1 cut(s) 462
BmgBI CACGTC 4 cut(s) 109, 1363, 2936, 3047
BmgT120I GGNCC 9 cut(s) 284, 552, 581, 610, 682, 976, 2249, 2615, 2851
BmiI GGNNCC 6 cut(s) 554, 582, 683, 1803, 2563, 3071
BmrFI CCNGG 2 cut(s) 288, 1844
BmrI ACTGGG 2 cut(s) 414, 2635
BmsI GCATC 7 cut(s) 906, 2055, 2203, 2323, 2681, 2738, 3014
BmtI GCTAGC 1 cut(s) 1356
BmuI ACTGGG 2 cut(s) 414, 2635
BpiI GAAGAC 1 cut(s) 860
BpmI CTGGAG 1 cut(s) 1826
Bpu10I CCTNAGC 1 cut(s) 2100
BpuEI CTTGAG 2 cut(s) 1629, 1957
Bsa29I ATCGAT 1 cut(s) 1893
BsaBI GATNNNNATC 1 cut(s) 831
BsaI GGTCTC 2 cut(s) 2541, 2819
BsaJI CCNNGG 5 cut(s) 299, 757, 979, 1825, 2809
Bsc4I CCNNNNNNNGG 7 cut(s) 1112, 1214, 2246, 2336, 2558, 2612, 3079
Bse1I ACTGG 5 cut(s) 420, 1353, 2539, 2641, 2653
Bse3DI GCAATG 2 cut(s) 52, 2548
Bse8I GATNNNNATC 1 cut(s) 831
BseBI CCWGG 2 cut(s) 288, 1844
BseCI ATCGAT 1 cut(s) 1893
BseDI CCNNGG 5 cut(s) 299, 757, 979, 1825, 2809
BseGI GGATG 5 cut(s) 77, 921, 1093, 1276, 2345
BseJI GATNNNNATC 1 cut(s) 831
BseLI CCNNNNNNNGG 7 cut(s) 1112, 1214, 2246, 2336, 2558, 2612, 3079
BseMI GCAATG 2 cut(s) 52, 2548
BseMII CTCAG 9 cut(s) 654, 1128, 1634, 1844, 1950, 2114, 2424, 2760, 3056
BseNI ACTGG 5 cut(s) 420, 1353, 2539, 2641, 2653
BseRI GAGGAG 1 cut(s) 1976
BseSI GKGCMC 2 cut(s) 1579, 2899
BseXI GCAGC 8 cut(s) 445, 583, 827, 1223, 1284, 1335, 2011, 2815
BseYI CCCAGC 1 cut(s) 1106
BsgI GTGCAG 1 cut(s) 85
BshFI GGCC 8 cut(s) 490, 612, 684, 978, 1207, 2216, 2250, 2271
BshNI GGYRCC 1 cut(s) 3069
BshVI ATCGAT 1 cut(s) 1893
BsiHKAI GWGCWC 2 cut(s) 469, 1579
BsiHKCI CYCGRG 1 cut(s) 462
BsiSI CCGG 2 cut(s) 1327, 2080
BslFI GGGAC 4 cut(s) 594, 778, 863, 1385
BslI CCNNNNNNNGG 7 cut(s) 1112, 1214, 2246, 2336, 2558, 2612, 3079
BsmAI GTCTC 7 cut(s) 43, 615, 793, 2541, 2730, 2819, 2942
BsmBI CGTCTC 1 cut(s) 2942
BsmFI GGGAC 4 cut(s) 594, 778, 863, 1385
BsnI GGCC 8 cut(s) 490, 612, 684, 978, 1207, 2216, 2250, 2271
Bso31I GGTCTC 2 cut(s) 2541, 2819
BsoBI CYCGRG 1 cut(s) 462
Bsp1286I GDGCHC 4 cut(s) 469, 1579, 2899, 3038
Bsp19I CCATGG 1 cut(s) 757
BspACI CCGC 7 cut(s) 14, 389, 560, 1110, 1214, 1566, 2022
BspANI GGCC 8 cut(s) 490, 612, 684, 978, 1207, 2216, 2250, 2271
BspCNI CTCAG 9 cut(s) 655, 1129, 1633, 1845, 1951, 2113, 2425, 2761, 3057
BspDI ATCGAT 1 cut(s) 1893
BspHI TCATGA 1 cut(s) 25
BspLI GGNNCC 6 cut(s) 554, 582, 683, 1803, 2563, 3071
BspMI ACCTGC 1 cut(s) 130
BspOI GCTAGC 1 cut(s) 1356
BspPI GGATC 6 cut(s) 24, 834, 1816, 2231, 2759, 3069
BspQI GCTCTTC 1 cut(s) 343
BspT107I GGYRCC 1 cut(s) 3069
BspTNI GGTCTC 2 cut(s) 2541, 2819
BsrDI GCAATG 2 cut(s) 52, 2548
BsrI ACTGG 5 cut(s) 420, 1353, 2539, 2641, 2653
BssECI CCNNGG 5 cut(s) 299, 757, 979, 1825, 2809
BssNAI GTATAC 1 cut(s) 2693
BssT1I CCWWGG 5 cut(s) 299, 757, 979, 1825, 2809
Bst1107I GTATAC 1 cut(s) 2693
Bst2UI CCWGG 2 cut(s) 288, 1844
Bst6I CTCTTC 4 cut(s) 343, 888, 1484, 1514
BstC8I GCNNGC 8 cut(s) 275, 686, 787, 1209, 1354, 1968, 2029, 2085
BstDSI CCRYGG 1 cut(s) 757
BstF5I GGATG 5 cut(s) 77, 921, 1093, 1276, 2345
BstHHI GCGC 2 cut(s) 1872, 2076
BstMAI GTCTC 7 cut(s) 43, 615, 793, 2541, 2730, 2819, 2942
BstMWI GCNNNNNNNGC 6 cut(s) 464, 568, 1289, 2021, 2080, 2598
BstNI CCWGG 2 cut(s) 288, 1844
BstNSI RCATGY 4 cut(s) 458, 2328, 2656, 2835
BstSCI CCNGG 2 cut(s) 286, 1842
BstSLI GKGCMC 2 cut(s) 1579, 2899
BstV1I GCAGC 8 cut(s) 445, 583, 827, 1223, 1284, 1335, 2011, 2815
BstV2I GAAGAC 1 cut(s) 860
BstX2I RGATCY 3 cut(s) 826, 2236, 3061
BstXI CCANNNNNNTGG 1 cut(s) 2648
BstYI RGATCY 3 cut(s) 826, 2236, 3061
BstZ17I GTATAC 1 cut(s) 2693
Bsu15I ATCGAT 1 cut(s) 1893
BsuI GTATCC 1 cut(s) 2944
BsuRI GGCC 8 cut(s) 490, 612, 684, 978, 1207, 2216, 2250, 2271
BsuTUI ATCGAT 1 cut(s) 1893
BtgI CCRYGG 1 cut(s) 757
BtrI CACGTC 4 cut(s) 109, 1363, 2936, 3047
BtsCI GGATG 5 cut(s) 77, 921, 1093, 1276, 2345
BtsI GCAGTG 2 cut(s) 676, 796
BtsIMutI CAGTG 8 cut(s) 676, 796, 1259, 2040, 2294, 2648, 2760, 2961
BveI ACCTGC 1 cut(s) 130
Cac8I GCNNGC 8 cut(s) 275, 686, 787, 1209, 1354, 1968, 2029, 2085
CciI TCATGA 1 cut(s) 25
CfoI GCGC 2 cut(s) 1872, 2076
Cfr13I GGNCC 9 cut(s) 284, 552, 581, 610, 682, 976, 2249, 2615, 2851
ClaI ATCGAT 1 cut(s) 1893
CseI GACGC 1 cut(s) 348
DraI TTTAAA 1 cut(s) 1080
EaeI YGGCCR 2 cut(s) 2214, 2269
Eam1104I CTCTTC 4 cut(s) 343, 888, 1484, 1514
EarI CTCTTC 4 cut(s) 343, 888, 1484, 1514
EciI GGCGGA 1 cut(s) 549
Eco130I CCWWGG 5 cut(s) 299, 757, 979, 1825, 2809
Eco24I GRGCYC 1 cut(s) 3038
Eco31I GGTCTC 2 cut(s) 2541, 2819
Eco47I GGWCC 5 cut(s) 284, 552, 581, 2615, 2851
Eco57I CTGAAG 5 cut(s) 171, 1092, 1362, 2265, 2895
Eco88I CYCGRG 1 cut(s) 462
EcoO109I RGGNCCY 4 cut(s) 552, 610, 976, 2249
EcoRI GAATTC 2 cut(s) 130, 2428
EcoRII CCWGG 2 cut(s) 286, 1842
EcoT14I CCWWGG 5 cut(s) 299, 757, 979, 1825, 2809
EcoT22I ATGCAT 1 cut(s) 705
EcoT38I GRGCYC 1 cut(s) 3038
ErhI CCWWGG 5 cut(s) 299, 757, 979, 1825, 2809
Esp3I CGTCTC 1 cut(s) 2942
FaqI GGGAC 4 cut(s) 594, 778, 863, 1385
FauI CCCGC 1 cut(s) 1207
FauNDI CATATG 4 cut(s) 355, 1378, 2017, 2116
FbaI TGATCA 2 cut(s) 970, 1147
FblI GTMKAC 1 cut(s) 2692
Fnu4HI GCNGC 9 cut(s) 459, 572, 816, 1212, 1298, 1324, 2022, 2025, 2829
FokI GGATG 5 cut(s) 84, 928, 1100, 1283, 2352
FriOI GRGCYC 1 cut(s) 3038
Fsp4HI GCNGC 9 cut(s) 459, 572, 816, 1212, 1298, 1324, 2022, 2025, 2829
GlaI GCGC 2 cut(s) 1871, 2075
GluI GCNGC 9 cut(s) 459, 572, 816, 1212, 1298, 1324, 2022, 2025, 2829
GsaI CCCAGC 1 cut(s) 1110
GsuI CTGGAG 1 cut(s) 1826
HaeIII GGCC 8 cut(s) 490, 612, 684, 978, 1207, 2216, 2250, 2271
HapII CCGG 2 cut(s) 1327, 2080
HgaI GACGC 1 cut(s) 348
HhaI GCGC 2 cut(s) 1872, 2076
Hin6I GCGC 2 cut(s) 1870, 2074
HinP1I GCGC 2 cut(s) 1870, 2074
HincII GTYRAC 1 cut(s) 174
HindII GTYRAC 1 cut(s) 174
HindIII AAGCTT 2 cut(s) 1074, 2085
HpaII CCGG 2 cut(s) 1327, 2080
HphI GGTGA 6 cut(s) 412, 782, 1615, 2324, 2595, 3029
Hpy166II GTNNAC 4 cut(s) 174, 1577, 2646, 2693
Hpy8I GTNNAC 4 cut(s) 174, 1577, 2646, 2693
HpyAV CCTTC 7 cut(s) 187, 348, 566, 1677, 1679, 2240, 2697
HpyCH4IV ACGT 5 cut(s) 108, 333, 1362, 2935, 3046
HpyF10VI GCNNNNNNNGC 6 cut(s) 464, 568, 1289, 2021, 2080, 2598
HpySE526I ACGT 5 cut(s) 108, 333, 1362, 2935, 3046
HspAI GCGC 2 cut(s) 1870, 2074
KpnI GGTACC 1 cut(s) 3073
Ksp22I TGATCA 2 cut(s) 970, 1147
LguI GCTCTTC 1 cut(s) 343
LmnI GCTCC 3 cut(s) 1264, 1845, 1963
Lsp1109I GCAGC 8 cut(s) 445, 583, 827, 1223, 1284, 1335, 2011, 2815
LweI GCATC 7 cut(s) 906, 2055, 2203, 2323, 2681, 2738, 3014
MaeII ACGT 5 cut(s) 108, 333, 1362, 2935, 3046
MfeI CAATTG 3 cut(s) 167, 1719, 2451
MflI RGATCY 3 cut(s) 826, 2236, 3061
MhlI GDGCHC 4 cut(s) 469, 1579, 2899, 3038
MlsI TGGCCA 2 cut(s) 2216, 2271
MluNI TGGCCA 2 cut(s) 2216, 2271
MlyI GAGTC 3 cut(s) 2114, 2157, 2731
MmeI TCCRAC 2 cut(s) 2637, 2891
Mox20I TGGCCA 2 cut(s) 2216, 2271
Mph1103I ATGCAT 1 cut(s) 705
MscI TGGCCA 2 cut(s) 2216, 2271
MslI CAYNNNNRTG 5 cut(s) 24, 107, 398, 995, 2780
Msp20I TGGCCA 2 cut(s) 2216, 2271
MspA1I CMGCKG 3 cut(s) 1110, 1214, 2024
MspI CCGG 2 cut(s) 1327, 2080
MspR9I CCNGG 2 cut(s) 288, 1844
MunI CAATTG 3 cut(s) 167, 1719, 2451
MvaI CCWGG 2 cut(s) 288, 1844
MwoI GCNNNNNNNGC 6 cut(s) 464, 568, 1289, 2021, 2080, 2598
NcoI CCATGG 1 cut(s) 757
NdeI CATATG 4 cut(s) 355, 1378, 2017, 2116
NheI GCTAGC 1 cut(s) 1352
NlaIV GGNNCC 6 cut(s) 554, 582, 683, 1803, 2563, 3071
NmuCI GTSAC 5 cut(s) 743, 770, 2090, 2511, 2790
NsiI ATGCAT 1 cut(s) 705
NspI RCATGY 4 cut(s) 458, 2328, 2656, 2835
OliI CACNNNNGTG 2 cut(s) 107, 398
PaeR7I CTCGAG 1 cut(s) 462
PagI TCATGA 1 cut(s) 25
PciSI GCTCTTC 1 cut(s) 343
PcsI WCGNNNNNNNCGW 1 cut(s) 1817
PflFI GACNNNGTC 1 cut(s) 2129
PflMI CCANNNNNTGG 1 cut(s) 2612
PkrI GCNGC 9 cut(s) 460, 573, 817, 1213, 1299, 1325, 2023, 2026, 2830
PleI GAGTC 3 cut(s) 2114, 2157, 2730
PpsI GAGTC 3 cut(s) 2114, 2157, 2730
PpuMI RGGWCCY 1 cut(s) 552
PshBI ATTAAT 1 cut(s) 384
PsiI TTATAA 2 cut(s) 516, 1833
Psp5II RGGWCCY 1 cut(s) 552
Psp6I CCWGG 2 cut(s) 286, 1842
PspFI CCCAGC 1 cut(s) 1106
PspGI CCWGG 2 cut(s) 286, 1842
PspN4I GGNNCC 6 cut(s) 554, 582, 683, 1803, 2563, 3071
PspPI GGNCC 9 cut(s) 284, 552, 581, 610, 682, 976, 2249, 2615, 2851
PspPPI RGGWCCY 1 cut(s) 552
PspXI VCTCGAGB 1 cut(s) 462
PsuI RGATCY 3 cut(s) 826, 2236, 3061
PsyI GACNNNGTC 1 cut(s) 2129
RseI CAYNNNNRTG 5 cut(s) 24, 107, 398, 995, 2780
SapI GCTCTTC 1 cut(s) 343
SatI GCNGC 9 cut(s) 459, 572, 816, 1212, 1298, 1324, 2022, 2025, 2829
Sau96I GGNCC 9 cut(s) 284, 552, 581, 610, 682, 976, 2249, 2615, 2851
ScaI AGTACT 1 cut(s) 589
SchI GAGTC 3 cut(s) 2114, 2157, 2731
ScrFI CCNGG 2 cut(s) 288, 1844
SduI GDGCHC 4 cut(s) 469, 1579, 2899, 3038
SfaNI GCATC 7 cut(s) 906, 2055, 2203, 2323, 2681, 2738, 3014
Sfr274I CTCGAG 1 cut(s) 462
SinI GGWCC 5 cut(s) 284, 552, 581, 2615, 2851
SlaI CTCGAG 1 cut(s) 462
SmiMI CAYNNNNRTG 5 cut(s) 24, 107, 398, 995, 2780
SmlI CTYRAG 3 cut(s) 462, 1644, 1936
SmoI CTYRAG 3 cut(s) 462, 1644, 1936
SsiI CCGC 7 cut(s) 14, 389, 560, 1110, 1214, 1566, 2022
SspI AATATT 3 cut(s) 382, 520, 2056
StyD4I CCNGG 2 cut(s) 286, 1842
StyI CCWWGG 5 cut(s) 299, 757, 979, 1825, 2809
TaiI ACGT 5 cut(s) 111, 336, 1365, 2938, 3049
TaqI TCGA 9 cut(s) 451, 463, 1156, 1249, 1672, 1811, 1820, 1893, 3057
TatI WGTACW 5 cut(s) 587, 1175, 1462, 2321, 2697
TauI GCSGC 1 cut(s) 2024
TscAI CASTG 8 cut(s) 676, 796, 1259, 2040, 2294, 2648, 2767, 2961
TseFI GTSAC 5 cut(s) 743, 770, 2090, 2511, 2790
TseI GCWGC 8 cut(s) 458, 571, 815, 1211, 1297, 1323, 2024, 2828
Tsp45I GTSAC 5 cut(s) 743, 770, 2090, 2511, 2790
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 8 cut(s) 676, 796, 1259, 2040, 2294, 2648, 2767, 2961
Tth111I GACNNNGTC 1 cut(s) 2129
Van91I CCANNNNNTGG 1 cut(s) 2612
VneI GTGCAC 1 cut(s) 1575
VpaK11BI GGWCC 5 cut(s) 284, 552, 581, 2615, 2851
VspI ATTAAT 1 cut(s) 384
XapI RAATTY 8 cut(s) 130, 194, 409, 510, 650, 1081, 1816, 2428
XbaI TCTAGA 2 cut(s) 326, 1046
XceI RCATGY 4 cut(s) 458, 2328, 2656, 2835
XcmI CCANNNNNNNNNTGG 1 cut(s) 1114
XhoI CTCGAG 1 cut(s) 462
XmiI GTMKAC 1 cut(s) 2692
ZrmI AGTACT 1 cut(s) 589
Zsp2I ATGCAT 1 cut(s) 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.