Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0006044

Physical Location & Seq

Genomic Coordinates Forward (+)

1883234 .. 1891083

Gene Structure 7850 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1026200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5184 bp

ATGGGAATACCTGATCGTTCACTACTAGATCAAATAGACCAAGTCAGGTCTTGGATTTCTGGAGGATCAAGCTATTTGCGGGTTTTGTCTGGTGAGTTTGATATGCCTTGTAACGGTTGCAAGATGTGTTGTGATTGCAATACAAACACCACCGGTATTGGTCCTAGATATCACTGCCAGAGCTGTGGTAGATGGATTTGTGGAAAGTGTATTCAGGGCTCCGAGTGGGATGGCATCGAGATCAGTGATGAGGATGGAGAGAGTAGTATTAAGTTCTGCAAGTTTTGTTCTTTGGCTAGATTAAGGAAGGAAGGTGGAAGGAAGAATAGTGAGAAAGTGCATCCGTCTGCCTCTCCCCGAGAAAGCCCTGAACCACCATCCCCCTGTTGTAGTGGTGAAACAGTCAAGTGTTATGCGGATAATGATGAATCCATTCGCGGTGATCATTTCGTAAAGATTTTAGAAGCCCATGATTGCGGTTATTCACCCCATGCTGAAAGGAGTATGACCTCGTTTAGTTCTCACCCATCTCCAATATCTGTTCGTCAGTCTATTAGCAGGAGTGACGAAGAGGAGGCAGAGGATTCTGGAAAGAACTTTGGCAGTCCTTTAAGTGAATATTGTGATGACAATTTGGACATAGATATAAGTAGTGTTAGTTCGAGAAATGAGTTTTACAGTTCAAGGTCACTGGGATCCAATCATTTTGATTGCCCATCTAGAATTTATTATGCTTCTAGTAGAGTTGGGCATTTTGTACAGCAGGGACGGGACGGAATCCTTGTGTCTCAGAATGATGGTCCCTTTGGTCAACAGACTAAGGCCGTTTTAAAAAGGCCTGAGAGAGGGACCGAGGATCCAGATATTACTGATGATTGCTCTGATGACCCATCAGTTATCCGTAATCAATATGAGAAGTCACAGAGGCCACTGGATTTTGAAAACAATGGTCTCATCTGGTATCCCCCTCCGCCTGATGATGAGAATGACGAGGCAGAGAGTAATTTCTTTTCTTATGATGATGAAGATGATGACGTTGGAGATTCAGGTGCAGTGTTTTCATCAAGCAGTAGCCTCTCGAGCATGTTTCCTGCAAAGGGGAAACAGAATGAGGGTAACAAGGAACCACTTAGAGATGTGGTACAGGGGCATTTTAGAGCTCTTGTATCACAACTGTTACAAGGTGAGGGTTTCATTGGTAAAGGGGATGGAGATGAGGACTGGCTTGACATAGTTACAACAATTGCGTGGCAAGCGGCAAATTTTGTTAAACCAGATACTAGCAGAGGAGGCAGTATGGATCCTGGTGATTATGTGAAAGTTAAATGTGTTGCATCAGGAAGTCCAAGTGACAGTACCCTTATAAAAGGAGTAGTTTGTACAAAGAATATAAGGCACAAACGCATGACCTCACAGTACAAAAATCCTAGATTACTTATTTTAGGGGGAGCACTTGAGTATCAGAAAATTCCAAACCAGTTGGCATCTTTTGACACATTGCTACATCAGGAAAACGATCATCTTAGGATGATTATCTCAAAGATAGAGGCTCTTCGCCCCAATGTTCTGCTGGTAGAGAAAAGTGTATCTTCGTATGCTCAAGACTGCCTGCTGGAAAAGGAAATATCATTAGTATTGAATGTCAAAAGGCCAGTATTGGAGCGCATAGCCCAGTGTACTGGTGCACTTATTACTCCATCAGTTGATGATATCCCTAAGACCCGTTTGGGACATTGTGAATTGTTCCGGTTAGAAAAAATTACTGAACAGCATGAGCCCGCCAATCAGTTTAACAAGAAACCACTGAAAACCCTGATGTTTTTTGAAGGGTGTCCAAGGCGTCTTTGCTGCACTGTTCTGCTGAAGGGTGCGTGTGTTGAAGAACTAAAGAAGATCAAGCATGTTGTCCAGTATTCAGTGTTTGCAGCTTACCATTTATCCCTTGAAACTTCCTTCCTTGCCGATGAGGGTGCTACTCTGCCCAAGACACCTTTGAGACACTCAATTACTATACCAGACAGGACAACAGCTGATGCGATTTCAGTAGTCCCTAATTCTCTTGCTTCGAGCAATTCCCAGGCTGTAACTTTTGCTTCTACTCAAGATGATAACATTTTGGGTCTGAAGCCGGAGATTGAAGGATTAGAATCTTTGCCTGGGCATCTTGATCCTGGACTTGATTTACCTGTGTCTAATGGTTCTGTTGACTGTGTGGTTGGAAATACATCTTCTGATGCTTACACTGATGATATAGGAAATAATGTGTTTTTGGGATCTTATCAATACAAGGATATAAATGGAGTCACTGTCCATTCTTCTGAAACAAAAGATCTCTCCCAACCAGAGTTGCAAGAAAGCTTGCCTCATGACTGGAGTCAGCATGAGGATAATGAGTTGACAAACTCTGAAAGGATTGATCACAATGAAGTTTCAAGTGAGTACTTCTCTTCTGCTGACACTCATCAGAGCATATTGGTATCCTTCTCAAGCCATTGTGTACTGAAGGGAACTGTTTGTGAACGCTCCCGACTTCTGCGCATAAAATTCTATGGTTGCTTTGATAAACCACTGGGAAGATATCTTCGTGATGACCTATTTGATCAGACGTCTTCGTGTCGAACTTGTAAAGAGCCAGCGGAGGCGCATGTTCTATGTTACACCCATCAGCAAGGAAACCTTACCATTAATGTTAGACGCCTTCCTTCACTAAAGCTACCTGGGGAGAGAGATGGTAAAATATGGATGTGGCACCGGTGTCTAAGGTGTGCCCAAATTGATGGAGTTCCACCAGCAACACGGAGAGTAGTCATGTCAGATGCTGCATGGGGGCTTTCTTTTGGAAAGTTCTTGGAACTGAGCTTTTCAAACCATGCAACTGCGAATCGTATTGCTACTTGTGGCCATTCATTACAACGGGATTGTCTCCGTTACTATGGGTTTGGGAGCATGGTTGCGTTCTTCCGATATTCCCCAATTGATATTCTTTCTGTCCATTTGCCCCCTTCAGTGCTTGAATTCAATGGCCAAGTTCAACCAGAGTGGATCAGACAAGAGGCAACAGAGCTTATGGGAAAAATGGAAACCATATATGCAGAGATTTCTGATGTACTTGACTGCATGGAGGAGAAAAATAGATCCTTGGGACATCAAATGTCAGGTGCAATTGAGTTACAGAACCACATTATGGAGCTGAAAGATCTGCTTAAGAAGGAAAGAAATGACTACATTGGTTTTCTGCAACCTGCTTTTGTGGAGACGTCAGAACCAGGCCAAATGCCTGTTTCCGACATTCTGGAGCTAAATCGCTTGAGACGTTCTCTTTTAATTGGTTCTCATGTTTGGGATCGCCAGCTTTATTCATTGGACTCGCTGCTTAAAGGGAATCCTGTTTCCATGGCTACTGATGGGGTTGTATCTTTTGCCCACCTGCAAGAGTTGATAAGTGGTCCATTTGACAAGGATGGCAGCCTTGACTATGGCTCTGAAGATAATGTATCTGAGTCTTCAAAATTTCAAGTGCCTTCTGTAGACATATCATGTCACTATAGCCGAGAGGAGGAAATGCACTCAGATAAGGAAATTGTTGGTGAGACATCCTATGAAGGCTTGTCCTCCCTTAAATCTACACTGTCCGAGAGAATAGATTCTGCATGGACTGGAACAGATCAACTTCTGGTGAAAGCTCAACCTCTTGATGCATCACATTTGACTGAACTTCAAGCTGGTGCTGTCAAGCATACTAGTCAAAGTGATGATCCTCCTTTTAAGAGGCTAATGTCGCCAGTGAGAGTTCAGTCTTTTGATTCTGTACTAGGATTTCAAGACAGGATTAGGAAGGGATCATTCCATGCTTCTGGAGATTATAGGAGTATGGTGAGACATCCTGTTTCTCGAGTCAGAAGGACTCTTTCCCAGGCATTGCCTCGAGAGGCACAGAAGTTGGATTCGATACTCAATTCTACACCCTCGTTTGTCTCCTCTGCATCTCAAATAGCTGATGGGGTTCGGTTGCTGCTTTCCCAGACAAGCAACAATGACATAGTTGTTGGTGTTTATGATAGCGAGCCCACTAGCATAATCTCATATGCCCTTAGTTCCAAGGATTATGAGGATTGGGTTACTGATAATTTGAATGAGCATCAGGCAGGCTGGAGTATTCATGAAAGCTTAAAAGAAGATCCTACGGCTTCTATCTTTTCACCCTGGCAGTCTTTTGGCTCTATGGACTTGGATTATATCCATTATGGAACTTATGGATCTGAAGATACTTCAGCTTCCATGGGTAACCTGTTTGCCGAAGCCAAAAGATCTCCACATCTGAGGATTTCTTTTGGGGACGAGTCCTCAAATGGGGTGGGAAAAGTTAGATTTTCTGTGACGTGTTATTTTGCAAAGCAGTTTGACTCTCTTAGGAAAAAGTGCTGCCCCAGTGAAGTGGATTTTGTACGATCTTTGAGCCGCTGCAAGAGGTGGAGTGCACAGGGGGGGAAGAGCAATGTGTATTTTGCTAAATCTCTGGATGATAGATTCATCATAAAACAAGTCACAAAGACGGAATTGGAATCCTTTCAGGAATTTGCACCTAAGTACTTCAAATATTTGACAGATTCCCTTAGCTCAGGAAGTCCAACTTGTATTGCCAAGGTTCTTGGTATATATCAGGTCACTGTTAAACATCTAAAGGGTGGGAAGGAAACTAAAATGGATTTGATGGTGATGGAGAACCTGTTCTTCAAAAGAAATATTTCAAGGGTATATGATCTCAAGGGATCTACTCGATCCCGTTATAATTCTGATACAACGGGGGAAGACAAAGTACTGTTAGATATGAATCTGTTGGAATCATTACGTACACAACCCATGTTTCTTGGAAGCAAAGCAAAGAGAAGCCTGGAAAGAGCTATTTGGAATGATACGTCATTTTTAGCGTCTGTCGATGTAATGGACTACTCACTGCTGGTTGGGGTGGACGATGAGCGGAAGGAGCTGGTCTTGGGGATCATAGATTTCATGAGACAATATACATGGGACAAGCATTTGGAGACATGGGTAAAGGCATCCGGGATACTTGGTGGTCCGAAGAATGCTGCTCCGACTATTATTTCCCCGAAGCAATACAAGAAACGATTCAGGAAGGCGATGACCACGTATTTTCTCACCGTGCCGGATCAATGGTCGTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0008150 GO:0008152 GO:0008289 GO:0009987 GO:0010008 GO:0010118 GO:0012505 GO:0016020 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0090332 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 PIP5K

Protein Analysis

1728

Amino Acids

192.95

Weight (kDa)

5.52

Isoelectric Point (pI)

48.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cpn60_TCP1	PF00118	401 - 651	2e-31	TCP-1/cpn60 chaperonin family
PIP5K	PF01504	1494 - 1661	5.2e-36	Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K	PF01504	1658 - 1716	2.6e-07	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006044)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71010
fragaria_vesca	FvH4_4g32320 FvH4_4g32320 FvH4_4g32320 FvH4_4g32320 FvH4_4g32320
malus_domestica	MD13G1026200.v1.1 MD16G1029100.v1.1
prunus_persica	Prupe.1G323700_v2.0.a1 Prupe.1G323700_v2.0.a1
pyrus_communis	pycom13g02450 pycom13g02470 pycom16g02480 pycom16g02500
rosa_chinensis	RchiOBHm_Chr4g0441111
rosa_laevigata	RLG00000006113
rosa_multiflora	Rmu_sc0006317.1_g000017 Rmu_sc0027558.1_g000002
rosa_roxburghii	Rroxscaffold_5G00382080
rosa_rugosa	Rorug04G0331100 Rorug04G0331200
rosa_samantha	Rh4AG382800 Rh4BG396600 Rh4CG411700 Rh4DG389400 Rh6CG153000
rosa_wichuraiana	Rw4G033040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1364, 4788
AarI	CACCTGC	1 cut(s)	3435
AatII	GACGTC	2 cut(s)	2609, 3260
Acc16I	TGCGCA	1 cut(s)	2537
Acc36I	ACCTGC	2 cut(s)	3250, 3435
AccB1I	GGYRCC	1 cut(s)	2748
AccB7I	CCANNNNNTGG	1 cut(s)	3184
AccBSI	CCGCTC	1 cut(s)	4978
AccI	GTMKAC	1 cut(s)	3528
AccII	CGCG	1 cut(s)	438
AcoI	YGGCCR	2 cut(s)	2899, 3022
AcsI	RAATTY	7 cut(s)	723, 1261, 1467, 2543, 3014, 3509, 4574
AcuI	CTGAAG	7 cut(s)	1883, 2144, 2522, 2988, 3504, 4254, 4281
AcyI	GRCGYC	4 cut(s)	1840, 2606, 2695, 3257
AfiI	CCNNNNNNNGG	6 cut(s)	113, 845, 1097, 3184, 3556, 4350
AflII	CTTAAG	1 cut(s)	3203
AflIII	ACRYGT	1 cut(s)	4379
AgeI	ACCGGT	2 cut(s)	152, 2751
AhlI	ACTAGT	1 cut(s)	3740
AjiI	CACGTC	1 cut(s)	4380
AjnI	CCWGG	9 cut(s)	1303, 2076, 2155, 2170, 2716, 3265, 3912, 4202, 4890
AloI	GAACNNNNNNTCC	2 cut(s)	4712, 4744
Alw21I	GWGCWC	4 cut(s)	1162, 1453, 1687, 4480
Alw44I	GTGCAC	2 cut(s)	1683, 4476
AlwNI	CAGNNNCTG	1 cut(s)	2819
Ama87I	CYCGRG	4 cut(s)	357, 1078, 3891, 3924
AoxI	GGCC	7 cut(s)	822, 836, 926, 1649, 2899, 3022, 3268
ApaLI	GTGCAC	2 cut(s)	1683, 4476
ApeKI	GCWGC	9 cut(s)	1848, 1925, 2819, 3370, 3465, 4012, 4422, 4461, 5087
ApoI	RAATTY	7 cut(s)	723, 1261, 1467, 2543, 3014, 3509, 4574
AseI	ATTAAT	1 cut(s)	2685
AsiGI	ACCGGT	2 cut(s)	152, 2751
Asp700I	GAANNNNTTC	6 cut(s)	432, 3643, 4566, 4727, 4744, 5126
AspLEI	GCGC	3 cut(s)	1665, 2538, 2644
AspS9I	GGNCC	5 cut(s)	161, 800, 849, 3446, 5075
AsuC2I	CCSGG	1 cut(s)	5062
AvaI	CYCGRG	4 cut(s)	357, 1078, 3891, 3924
AvaII	GGWCC	5 cut(s)	161, 800, 849, 3446, 5075
BaeGI	GKGCMC	3 cut(s)	1687, 2770, 4480
BaeI	ACNNNNGTAYC	2 cut(s)	1442, 1475
BalI	TGGCCA	2 cut(s)	2901, 3024
BamHI	GGATCC	3 cut(s)	695, 856, 1300
BanI	GGYRCC	1 cut(s)	2748
BanII	GRGCYC	4 cut(s)	221, 1162, 1779, 4068
BarI	GAAGNNNNNNTAC	2 cut(s)	4800, 4832
BbsI	GAAGAC	3 cut(s)	2601, 3495, 4815
Bbv12I	GWGCWC	4 cut(s)	1162, 1453, 1687, 4480
BbvI	GCAGC	9 cut(s)	1835, 1937, 2806, 3357, 3477, 3999, 4409, 4448, 5074
BceAI	ACGGC	2 cut(s)	809, 4200
BcgI	CGANNNNNNTGC	2 cut(s)	1952, 1986
BciT130I	CCWGG	9 cut(s)	1305, 2078, 2157, 2172, 2718, 3267, 3914, 4204, 4892
BciVI	GTATCC	3 cut(s)	972, 2488, 5058
BclI	TGATCA	3 cut(s)	442, 2416, 2599
BcnI	CCSGG	1 cut(s)	5062
BcuI	ACTAGT	1 cut(s)	3740
BfmI	CTRYAG	2 cut(s)	3525, 3544
BfrI	CTTAAG	1 cut(s)	3203
BfuAI	ACCTGC	2 cut(s)	3250, 3435
BfuI	GTATCC	3 cut(s)	972, 2488, 5058
BglII	AGATCT	3 cut(s)	2329, 3196, 4307
BmcAI	AGTACT	3 cut(s)	2441, 4589, 4818
Bme18I	GGWCC	5 cut(s)	161, 800, 849, 3446, 5075
BmeT110I	CYCGRG	4 cut(s)	357, 1078, 3891, 3924
BmgBI	CACGTC	1 cut(s)	4380
BmgT120I	GGNCC	5 cut(s)	161, 800, 849, 3446, 5075
BmiI	GGNNCC	8 cut(s)	220, 697, 802, 850, 858, 1125, 1302, 2750
BmrI	ACTGGG	4 cut(s)	701, 1666, 2579, 4422
BmuI	ACTGGG	4 cut(s)	701, 1666, 2579, 4422
BoxI	GACNNNNGTC	1 cut(s)	2373
BpiI	GAAGAC	3 cut(s)	2601, 3495, 4815
BplI	GAGNNNNNCTC	4 cut(s)	2429, 2461, 3301, 3333
BpmI	CTGGAG	5 cut(s)	81, 2392, 3314, 3876, 4171
Bpu10I	CCTNAGC	2 cut(s)	4613, 4618
BpuEI	CTTGAG	6 cut(s)	1475, 1584, 2085, 2470, 3328, 4748
BpuMI	CCSGG	1 cut(s)	5062
BsaAI	YACGTR	2 cut(s)	4850, 5148
BsaBI	GATNNNNATC	3 cut(s)	1532, 2146, 4830
BsaHI	GRCGYC	4 cut(s)	1840, 2606, 2695, 3257
BsaI	GGTCTC	1 cut(s)	956
BsaWI	WCCGGW	3 cut(s)	152, 1746, 2751
BsaXI	ACNNNNNCTCC	4 cut(s)	2292, 2322, 4712, 4742
Bsc4I	CCNNNNNNNGG	6 cut(s)	113, 845, 1097, 3184, 3556, 4350
Bse118I	RCCGGY	2 cut(s)	152, 2751
Bse3DI	GCAATG	3 cut(s)	1496, 3917, 4501
Bse8I	GATNNNNATC	3 cut(s)	1532, 2146, 4830
BseBI	CCWGG	9 cut(s)	1305, 2078, 2157, 2172, 2718, 3267, 3914, 4204, 4892
BseJI	GATNNNNATC	3 cut(s)	1532, 2146, 4830
BseLI	CCNNNNNNNGG	6 cut(s)	113, 845, 1097, 3184, 3556, 4350
BseMI	GCAATG	3 cut(s)	1496, 3917, 4501
BseMII	CTCAG	7 cut(s)	803, 831, 2846, 3489, 3582, 4310, 4632
BseRI	GAGGAG	5 cut(s)	587, 1302, 3137, 3569, 3967
BseSI	GKGCMC	3 cut(s)	1687, 2770, 4480
BseXI	GCAGC	9 cut(s)	1835, 1937, 2806, 3357, 3477, 3999, 4409, 4448, 5074
BsgI	GTGCAG	2 cut(s)	1071, 1834
Bsh1236I	CGCG	1 cut(s)	438
BshFI	GGCC	7 cut(s)	824, 838, 928, 1651, 2901, 3024, 3270
BshNI	GGYRCC	1 cut(s)	2748
BshTI	ACCGGT	2 cut(s)	152, 2751
BsiHKAI	GWGCWC	4 cut(s)	1162, 1453, 1687, 4480
BsiHKCI	CYCGRG	4 cut(s)	357, 1078, 3891, 3924
BsiSI	CCGG	6 cut(s)	153, 1747, 2129, 2752, 5061, 5165
BslFI	GGGAC	9 cut(s)	780, 785, 786, 862, 1743, 2033, 3156, 4349, 5042
BslI	CCNNNNNNNGG	6 cut(s)	113, 845, 1097, 3184, 3556, 4350
BsmBI	CGTCTC	2 cut(s)	3248, 3304
BsmFI	GGGAC	9 cut(s)	780, 785, 786, 862, 1743, 2033, 3156, 4349, 5042
BsmI	GAATGC	1 cut(s)	5089
BsnI	GGCC	7 cut(s)	824, 838, 928, 1651, 2901, 3024, 3270
Bso31I	GGTCTC	1 cut(s)	956
BsoBI	CYCGRG	4 cut(s)	357, 1078, 3891, 3924
Bsp1286I	GDGCHC	8 cut(s)	221, 1162, 1453, 1687, 1779, 2770, 4068, 4480
Bsp1407I	TGTACA	2 cut(s)	757, 1379
Bsp19I	CCATGG	2 cut(s)	3393, 4278
BspANI	GGCC	7 cut(s)	824, 838, 928, 1651, 2901, 3024, 3270
BspCNI	CTCAG	7 cut(s)	802, 832, 2847, 3490, 3581, 4311, 4631
BspFNI	CGCG	1 cut(s)	438
BspHI	TCATGA	4 cut(s)	2365, 4159, 5010, 5180
BspLI	GGNNCC	8 cut(s)	220, 697, 802, 850, 858, 1125, 1302, 2750
BspMI	ACCTGC	2 cut(s)	3250, 3435
BspQI	GCTCTTC	2 cut(s)	1557, 4484
BspT107I	GGYRCC	1 cut(s)	2748
BspTI	CTTAAG	1 cut(s)	3203
BspTNI	GGTCTC	1 cut(s)	956
BsrBI	CCGCTC	1 cut(s)	4978
BsrDI	GCAATG	3 cut(s)	1496, 3917, 4501
BsrFI	RCCGGY	2 cut(s)	152, 2751
BsrGI	TGTACA	2 cut(s)	757, 1379
BssAI	RCCGGY	2 cut(s)	152, 2751
BssNI	GRCGYC	4 cut(s)	1840, 2606, 2695, 3257
BssT1I	CCWWGG	6 cut(s)	1835, 3138, 3393, 4098, 4278, 4641
Bst2UI	CCWGG	9 cut(s)	1305, 2078, 2157, 2172, 2718, 3267, 3914, 4204, 4892
Bst6I	CTCTTC	4 cut(s)	564, 1557, 2452, 4484
BstACI	GRCGYC	4 cut(s)	1840, 2606, 2695, 3257
BstAFI	CTTAAG	1 cut(s)	3203
BstAUI	TGTACA	2 cut(s)	757, 1379
BstBAI	YACGTR	2 cut(s)	4850, 5148
BstC8I	GCNNGC	8 cut(s)	1254, 1610, 1779, 2360, 2634, 3350, 4064, 4147
BstDSI	CCRYGG	2 cut(s)	3393, 4278
BstEII	GGTNACC	1 cut(s)	4283
BstENI	CCTNNNNNAGG	1 cut(s)	843
BstFNI	CGCG	1 cut(s)	438
BstHHI	GCGC	3 cut(s)	1665, 2538, 2644
BstMWI	GCNNNNNNNGC	5 cut(s)	1080, 1253, 1290, 3778, 4984
BstNI	CCWGG	9 cut(s)	1305, 2078, 2157, 2172, 2718, 3267, 3914, 4204, 4892
BstNSI	RCATGY	3 cut(s)	1087, 1904, 2648
BstPAI	GACNNNNGTC	1 cut(s)	2373
BstPI	GGTNACC	1 cut(s)	4283
BstSFI	CTRYAG	2 cut(s)	3525, 3544
BstSLI	GKGCMC	3 cut(s)	1687, 2770, 4480
BstSNI	TACGTA	1 cut(s)	4850
BstUI	CGCG	1 cut(s)	438
BstV1I	GCAGC	9 cut(s)	1835, 1937, 2806, 3357, 3477, 3999, 4409, 4448, 5074
BstV2I	GAAGAC	3 cut(s)	2601, 3495, 4815
BstXI	CCANNNNNNTGG	5 cut(s)	185, 1679, 2777, 3854, 4435
BsuI	GTATCC	3 cut(s)	972, 2488, 5058
BsuRI	GGCC	7 cut(s)	824, 838, 928, 1651, 2901, 3024, 3270
BtgI	CCRYGG	2 cut(s)	3393, 4278
BtgZI	GCGATG	1 cut(s)	5153
BtrI	CACGTC	1 cut(s)	4380
BtsI	GCAGTG	3 cut(s)	172, 1059, 4952
BveI	ACCTGC	2 cut(s)	3250, 3435
Cac8I	GCNNGC	8 cut(s)	1254, 1610, 1779, 2360, 2634, 3350, 4064, 4147
CaiI	CAGNNNCTG	1 cut(s)	2819
CciI	TCATGA	4 cut(s)	2365, 4159, 5010, 5180
CfoI	GCGC	3 cut(s)	1665, 2538, 2644
Cfr10I	RCCGGY	2 cut(s)	152, 2751
Cfr13I	GGNCC	5 cut(s)	161, 800, 849, 3446, 5075
CseI	GACGC	3 cut(s)	1829, 2703, 4917
CspAI	ACCGGT	2 cut(s)	152, 2751
CspCI	CAANNNNNGTGG	6 cut(s)	139, 174, 3020, 3055, 4335, 4370
DraI	TTTAAA	1 cut(s)	831
EaeI	YGGCCR	2 cut(s)	2899, 3022
Eam1104I	CTCTTC	4 cut(s)	564, 1557, 2452, 4484
EarI	CTCTTC	4 cut(s)	564, 1557, 2452, 4484
EciI	GGCGGA	1 cut(s)	960
Ecl136II	GAGCTC	1 cut(s)	1160
Eco105I	TACGTA	1 cut(s)	4850
Eco130I	CCWWGG	6 cut(s)	1835, 3138, 3393, 4098, 4278, 4641
Eco147I	AGGCCT	1 cut(s)	838
Eco24I	GRGCYC	4 cut(s)	221, 1162, 1779, 4068
Eco31I	GGTCTC	1 cut(s)	956
Eco32I	GATATC	3 cut(s)	170, 1711, 2579
Eco47I	GGWCC	5 cut(s)	161, 800, 849, 3446, 5075
Eco53kI	GAGCTC	1 cut(s)	1160
Eco57I	CTGAAG	7 cut(s)	1883, 2144, 2522, 2988, 3504, 4254, 4281
Eco88I	CYCGRG	4 cut(s)	357, 1078, 3891, 3924
Eco91I	GGTNACC	1 cut(s)	4283
EcoICRI	GAGCTC	1 cut(s)	1160
EcoNI	CCTNNNNNAGG	1 cut(s)	843
EcoO65I	GGTNACC	1 cut(s)	4283
EcoRI	GAATTC	1 cut(s)	3014
EcoRII	CCWGG	9 cut(s)	1303, 2076, 2155, 2170, 2716, 3265, 3912, 4202, 4890
EcoRV	GATATC	3 cut(s)	170, 1711, 2579
EcoT14I	CCWWGG	6 cut(s)	1835, 3138, 3393, 4098, 4278, 4641
EcoT22I	ATGCAT	1 cut(s)	3700
EcoT38I	GRGCYC	4 cut(s)	221, 1162, 1779, 4068
ErhI	CCWWGG	6 cut(s)	1835, 3138, 3393, 4098, 4278, 4641
Esp3I	CGTCTC	2 cut(s)	3248, 3304
FalI	AAGNNNNNCTT	8 cut(s)	1573, 1605, 2661, 2693, 3186, 3218, 4615, 4647
FaqI	GGGAC	9 cut(s)	780, 785, 786, 862, 1743, 2033, 3156, 4349, 5042
FauI	CCCGC	2 cut(s)	72, 1786
FauNDI	CATATG	1 cut(s)	4084
FbaI	TGATCA	3 cut(s)	442, 2416, 2599
FblI	GTMKAC	1 cut(s)	3528
FriOI	GRGCYC	4 cut(s)	221, 1162, 1779, 4068
FspI	TGCGCA	1 cut(s)	2537
GlaI	GCGC	3 cut(s)	1664, 2537, 2643
GsuI	CTGGAG	5 cut(s)	81, 2392, 3314, 3876, 4171
HaeIII	GGCC	7 cut(s)	824, 838, 928, 1651, 2901, 3024, 3270
HapII	CCGG	6 cut(s)	153, 1747, 2129, 2752, 5061, 5165
HgaI	GACGC	3 cut(s)	1829, 2703, 4917
HhaI	GCGC	3 cut(s)	1665, 2538, 2644
Hin1I	GRCGYC	4 cut(s)	1840, 2606, 2695, 3257
Hin6I	GCGC	3 cut(s)	1663, 2536, 2642
HinP1I	GCGC	3 cut(s)	1663, 2536, 2642
HincII	GTYRAC	3 cut(s)	812, 2206, 2397
HindII	GTYRAC	3 cut(s)	812, 2206, 2397
HindIII	AAGCTT	2 cut(s)	2356, 4165
HpaII	CCGG	6 cut(s)	153, 1747, 2129, 2752, 5061, 5165
HpyCH4IV	ACGT	8 cut(s)	1035, 2606, 3257, 3313, 4379, 4849, 4916, 5147
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1080, 1253, 1290, 3778, 4984
HpySE526I	ACGT	8 cut(s)	1035, 2606, 3257, 3313, 4379, 4849, 4916, 5147
Hsp92I	GRCGYC	4 cut(s)	1840, 2606, 2695, 3257
HspAI	GCGC	3 cut(s)	1663, 2536, 2642
Ksp22I	TGATCA	3 cut(s)	442, 2416, 2599
LguI	GCTCTTC	2 cut(s)	1557, 4484
LmnI	GCTCC	9 cut(s)	224, 1448, 1660, 2528, 2943, 3187, 3295, 4984, 5095
Lsp1109I	GCAGC	9 cut(s)	1835, 1937, 2806, 3357, 3477, 3999, 4409, 4448, 5074
MaeII	ACGT	8 cut(s)	1035, 2606, 3257, 3313, 4379, 4849, 4916, 5147
MbiI	CCGCTC	1 cut(s)	4978
MfeI	CAATTG	3 cut(s)	1242, 2973, 3162
MhlI	GDGCHC	8 cut(s)	221, 1162, 1453, 1687, 1779, 2770, 4068, 4480
MlsI	TGGCCA	2 cut(s)	2901, 3024
MluNI	TGGCCA	2 cut(s)	2901, 3024
MlyI	GAGTC	8 cut(s)	2310, 2383, 3359, 3509, 3898, 3903, 4349, 4397
MmeI	TCCRAC	7 cut(s)	1018, 2197, 3309, 3921, 4652, 4818, 5117
Mox20I	TGGCCA	2 cut(s)	2901, 3024
Mph1103I	ATGCAT	1 cut(s)	3700
MroXI	GAANNNNTTC	6 cut(s)	432, 3643, 4566, 4727, 4744, 5126
MscI	TGGCCA	2 cut(s)	2901, 3024
Msp20I	TGGCCA	2 cut(s)	2901, 3024
MspA1I	CMGCKG	3 cut(s)	2030, 2636, 4461
MspCI	CTTAAG	1 cut(s)	3203
MspI	CCGG	6 cut(s)	153, 1747, 2129, 2752, 5061, 5165
MunI	CAATTG	3 cut(s)	1242, 2973, 3162
Mva1269I	GAATGC	1 cut(s)	5089
MvaI	CCWGG	9 cut(s)	1305, 2078, 2157, 2172, 2718, 3267, 3914, 4204, 4892
MvnI	CGCG	1 cut(s)	438
MwoI	GCNNNNNNNGC	5 cut(s)	1080, 1253, 1290, 3778, 4984
NciI	CCSGG	1 cut(s)	5062
NcoI	CCATGG	2 cut(s)	3393, 4278
NdeI	CATATG	1 cut(s)	4084
NlaIV	GGNNCC	8 cut(s)	220, 697, 802, 850, 858, 1125, 1302, 2750
NmeAIII	GCCGAG	1 cut(s)	3575
NmuCI	GTSAC	9 cut(s)	563, 687, 918, 1349, 2302, 3539, 4375, 4543, 4663
NsbI	TGCGCA	1 cut(s)	2537
NsiI	ATGCAT	1 cut(s)	3700
NspI	RCATGY	3 cut(s)	1087, 1904, 2648
PaeR7I	CTCGAG	3 cut(s)	1078, 3891, 3924
PagI	TCATGA	4 cut(s)	2365, 4159, 5010, 5180
PaqCI	CACCTGC	1 cut(s)	3435
PceI	AGGCCT	1 cut(s)	838
PciSI	GCTCTTC	2 cut(s)	1557, 4484
PcsI	WCGNNNNNNNCGW	1 cut(s)	3310
PctI	GAATGC	1 cut(s)	5089
PdmI	GAANNNNTTC	6 cut(s)	432, 3643, 4566, 4727, 4744, 5126
PflFI	GACNNNGTC	1 cut(s)	41
PflMI	CCANNNNNTGG	1 cut(s)	3184
PfoI	TCCNGGA	2 cut(s)	2170, 5060
PinAI	ACCGGT	2 cut(s)	152, 2751
PleI	GAGTC	8 cut(s)	2309, 2382, 3359, 3508, 3898, 3902, 4348, 4397
PpsI	GAGTC	8 cut(s)	2309, 2382, 3359, 3508, 3898, 3902, 4348, 4397
Ppu21I	YACGTR	2 cut(s)	4850, 5148
PshAI	GACNNNNGTC	1 cut(s)	2373
PshBI	ATTAAT	1 cut(s)	2685
PsiI	TTATAA	2 cut(s)	1364, 4788
Psp124BI	GAGCTC	1 cut(s)	1162
Psp6I	CCWGG	9 cut(s)	1303, 2076, 2155, 2170, 2716, 3265, 3912, 4202, 4890
PspEI	GGTNACC	1 cut(s)	4283
PspGI	CCWGG	9 cut(s)	1303, 2076, 2155, 2170, 2716, 3265, 3912, 4202, 4890
PspN4I	GGNNCC	8 cut(s)	220, 697, 802, 850, 858, 1125, 1302, 2750
PspPI	GGNCC	5 cut(s)	161, 800, 849, 3446, 5075
PsrI	GAACNNNNNNTAC	2 cut(s)	643, 675
PstNI	CAGNNNCTG	1 cut(s)	2819
PsyI	GACNNNGTC	1 cut(s)	41
PvuII	CAGCTG	1 cut(s)	2030
SacI	GAGCTC	1 cut(s)	1162
SapI	GCTCTTC	2 cut(s)	1557, 4484
Sau96I	GGNCC	5 cut(s)	161, 800, 849, 3446, 5075
ScaI	AGTACT	3 cut(s)	2441, 4589, 4818
SchI	GAGTC	8 cut(s)	2310, 2383, 3359, 3509, 3898, 3903, 4349, 4397
SduI	GDGCHC	8 cut(s)	221, 1162, 1453, 1687, 1779, 2770, 4068, 4480
SfcI	CTRYAG	2 cut(s)	3525, 3544
Sfr274I	CTCGAG	3 cut(s)	1078, 3891, 3924
SgrAI	CRCCGGYG	1 cut(s)	2751
SinI	GGWCC	5 cut(s)	161, 800, 849, 3446, 5075
SlaI	CTCGAG	3 cut(s)	1078, 3891, 3924
SnaBI	TACGTA	1 cut(s)	4850
SpeI	ACTAGT	1 cut(s)	3740
SseBI	AGGCCT	1 cut(s)	838
SspI	AATATT	3 cut(s)	620, 4598, 4744
SstI	GAGCTC	1 cut(s)	1162
StuI	AGGCCT	1 cut(s)	838
StyI	CCWWGG	6 cut(s)	1835, 3138, 3393, 4098, 4278, 4641
TaiI	ACGT	8 cut(s)	1038, 2609, 3260, 3316, 4382, 4852, 4919, 5150
TaqII	GACCGA	1 cut(s)	866
TauI	GCSGC	2 cut(s)	1259, 4461
TseFI	GTSAC	9 cut(s)	563, 687, 918, 1349, 2302, 3539, 4375, 4543, 4663
TseI	GCWGC	9 cut(s)	1848, 1925, 2819, 3370, 3465, 4012, 4422, 4461, 5087
Tsp45I	GTSAC	9 cut(s)	563, 687, 918, 1349, 2302, 3539, 4375, 4543, 4663
TspGWI	ACGGA	6 cut(s)	333, 789, 890, 2812, 2915, 4568
Tth111I	GACNNNGTC	1 cut(s)	41
Van91I	CCANNNNNTGG	1 cut(s)	3184
Vha464I	CTTAAG	1 cut(s)	3203
VneI	GTGCAC	2 cut(s)	1683, 4476
VpaK11BI	GGWCC	5 cut(s)	161, 800, 849, 3446, 5075
VspI	ATTAAT	1 cut(s)	2685
XagI	CCTNNNNNAGG	1 cut(s)	843
XapI	RAATTY	7 cut(s)	723, 1261, 1467, 2543, 3014, 3509, 4574
XbaI	TCTAGA	1 cut(s)	719
XceI	RCATGY	3 cut(s)	1087, 1904, 2648
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1567
XhoI	CTCGAG	3 cut(s)	1078, 3891, 3924
XmiI	GTMKAC	1 cut(s)	3528
XmnI	GAANNNNTTC	6 cut(s)	432, 3643, 4566, 4727, 4744, 5126
ZraI	GACGTC	2 cut(s)	2607, 3258
ZrmI	AGTACT	3 cut(s)	2441, 4589, 4818
Zsp2I	ATGCAT	1 cut(s)	3700

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.