Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0006044

Physical Location & Seq

Genomic Coordinates Forward (+)

2073549 .. 2081615

Gene Structure 8067 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1029100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5148 bp

ATGTGTTGTGATTGCAATACAAACACCACCGGTATTGGTTCTAGATATCACTGTCAGAGCTGTGGCAGATGGATTTGTGGAAAGTGTATTCAGGGCTCTGAGTGGGATAGCATCAAGAGCAATGATGAGGTTGGAGGGAGTACTATTAAGTACTGCAAGTTTTGTTCTTTGGCTGGATTAAGGAAGGAAGGTGGAAGGAATAATAGTGAGAAAGTGCATCCGTCTGCCTCTCCCCAAGAAAACCCTGAACCACCATCACCCTGTTGTAGTGGTGAAACAGTCAAGTGTTCTGTGGATAATGATGAATCTATTCACGGTGATCATTTCTCAAAGATTTTGGAAGCCCATGATTGCAGTTATTCTCCCCATGCTGTGAGGAGTATGACCTCACTTAGTTCTCACCCATCTCCAATATCTGTTCATCACTCTATTAGCAGGAGTGACGAAGAGGAGGCAGAGGATTCTGGAAAGAACTTTTGCAGTCCTTTAAGTGAATATTGTGATGACAATTCGGACATAGATTTAAGTAGTGTTAGTTCTAGAAAAGAGTTTTTCAGGTCAAGGTCACTGGGATCCCATCATTTTGATTGCCCGTCTAGAATTTATTATACTTCTAGTAGAGTTGGGCATTCTGTACAGCAGGGGCAGGAGGGAATCCCTGTGTCTCAGAATGATGGTCCCTTTGGTCAACAGAATAAGGCCGTTTTCAAAAGGCCTGAGAGAGGGAGCAGAGATCCAGATATTACTGATGATTGCTCTGATGACCCGTCAGTTATCCGTAGTCAATATGAGAAGTCACAGCGGCCACTGGATTTTGAAAACAATGGTCTCATCTGGTATCCCCCTCCGCCTGATGATGAGAATGATGAGGCAGAGTGTAATATCTTTTCTTATGATGATGAAGATGATGATATTGGAGATTCCGGTGCTGTGTTTTCATCAAGCAGTAGCCTTTCGAGCATGTTTCCTGCAAAGGAGAAACAGAATGAGGGTAACAAGGAACCACTTAGAGCTGTGGTACATGGGCATTTTAGAGCTCTTGTATCACAACTGTTACAAGGTGAGGGTTTCATGGATAAAGGGGATGGAGATGAGGACTGGCTTGACATAGTTACAACAATTGCATGGCAAGCAGCAAATTTTGTTAAACCAGATACAAGCAGAGGAGGCAGTATGGATCCTGGTGATTATGTGAAAGTTAAATGTATTGCATCAGGAAGTCCAAGTGACAGTACCCTTATAAAAGGAGTAGTTTGTACAAAGAATATAAAGCATAAGCGCATGACCTCACAGTACAAAAATCCTAGATTACTTATTTTAGGGGGAGCACTTGAGTATCAGAAAATTCCGAACAAGTTGGCATCTTTCGACACATTACTGCATCAGGAAAACGATCATCTTAGGATGATTATCTCAAAGATAGAGGCTCTTCACCCCAATGTTCTGCTGGTAGAAAAAAGTGTATCTTCGTATGCTCAAGACTACCTGCTGGAAAAGGAAATATCGTTAGTATTGAATGTTAAAAGGTCATTACTGGAGCGTATAGCACAATGTACTGGTGCACTTATTACTCCATCAATTGACGATATCCCTAAGACTCGTTTGGGGCATTGTGAGCTGTTCCGGTTAGAAAAAATTTCTGAACAGCATGAGCCAGCCAATCAGTTTAACAAGAAACCAGTGAAAACCCTGATGTTTTTCGAAGGGTGTCCAAGGCGTCTTTGCTGCACAGTTCTGCTGAAGGGTGCTTGTGTTGAAGAACTAAAGAAGATCAAGCATGTTGTCCAGTATGCAGTGTTTGCAGCTTACCATTTATCCCTTGAAACTTCCTTCCTTGCTGATGAGGGTGCTACTCTGCCCAAGACGACTCTGAGACACCCAGTTACTATACCAGACAGGACAACAGCTGATGCGAATTCAGTAATCCCTAATTCCCTTGCTTTGAGCAATTCCCAGGCTGTACCTTTTACTTCTGTTCAAGATGATAATATTTTGGGTCTGAAGCCGGAAATTGAAGGATTAGAGTCTTTGCCCGGACATCTTTATCATGGACTTGATTTTCCTGTGTCTTATGGTGACTCTGTGGTTGGAACTACATCTTCTGATGCTTATACTGATGATATAGGAAATAATGTGATTTTGGGATCCTCGTATCAATACAAGGATATAAATGGGATCACAGTCCATTCTTCTGAAACAAAAGACCTTTCCCAACCAGAGTTGCAAGAAAGCTTGCCTCATGACTGGAGTCAGCATGAGGATAATGAGTTGATAAACTCTGAAAGGATTGATCACAATCAAGTTTCAAGTGAGTACTTCTCTTCTGCTGACACTCATCAGAGCATATTGGTATCCTTTTCAAGTTATTGTGTACTGAAAGGAACTGTTTGTGAACGCTCCCAACTTCTGCGCATAAAATTCTATGGTTGCTTTGATAAGCCACTGGGCAGATATCTTCGTGATGACCTATTTGATCAGACATCTTCCTGTCGGACTTGTAAAGAGACAGCCGAGTCTCATCTTCTATGTTACACCCATCAGCAAGGAAACCTTACCATTAATGTTAGACGCCTTCCTTCACTAAAGCTACCTGGGGAGAGAGATGGTAAAATATGGATGTGGCACCGGTGTCTAAGGTGTGCCCATATTGATGGAGTTCCACCAGCAACACGTAGAGTAGTCATGTCAGATGCTGCCTGGGGGCTTTCTTTTGGAAAGTTCTTGGAACTGAGCTTTTCAAATCATGCAACTGCGAATCGTGTTGCTACTTGTGGCCATTCATTACAACGGGATTGTCTTCGTTACTATGGGTTTGGAAGCATGGTTGCGTTCTTCCGATATTCCGCGATTGATATTCTTTCTGTCCATTTGCCCCCTTCAGTGCTTGAATTCAATGGCCAAGTCCAACCAGAGTGGATAAGAAAAGAGGCAACAGAGCTTATGGGAAAAATGGAAACCATATATGCAGAGATATCTGATGTACTCGACTGCATGGAGGAGAAAAATAGATCTTTTGGATGTGAAATGTCAGGTACAATTGAATTACAGCACCACATCTTGGAGCTGAAAGATCTGCTTAAGAAGGAAAGAAATGACTACATTGGTTTTCTGCAACCTGCTTTTGTGGAGACTTCTGAACCAGGACAAATGCCTGTTGTAGACGTTCTGGAGCTAAATCGCTTGAGACGTTCTCTTTTAATTGGTTCTCATGTTTGGGATCGCCAGCTATATTCATTGGACTCACTGCTTAAAAGGACTCCTGTTTCCATGGCTACTGATGAGGATGTATCTTTTGCCCACCTGCAAGAGTTGATAAGTGATCCATCTGGTAAGGATGTCAGTCTTGACTATGGCCATGAAGATCATGTGTCTGAGTCTTCAAAATTCCAAGTGCCTCCCGGCAATGATCTACCAACAGATAAAGAACCTAATGTACCACCACTTGAACCTTCTGAAGACGTATCCCACCATTATAGCCGAGACGACGAGATGCATTCAGATAAGGAAATTGTTAATAAGACATCCTGCAAAGGCTTTTCCTCCCTTAAATCTACACTGTCTGAGAGAATAGATTCTGCATGGACTGGGACTGATCAACTTGTGGTGAAAGCTCAACCACTTGGTACATCACATTTGACTGAAGTTCAAGCTCGTGCTGTCAAGCATACTAGTCAAAATGATGATCCCCCTCTTAGGAGGCTAATGTCGCCAGTGAGAGTTCACTCTTTTGATTCTGCCCTAAGATTCCGAGACAGGATTAGGAAGGGATTGCCCCCTTCTTCATTGCATTTGTCGACGCTTAGATCATTCCACGCGTCTGGAGATTACAGGAGTATGGTGAGAGATCCTGTTTCTAGTGTAAGAAGGACTCTTTCCCAGGCATTGCCACGCGAGGCACAAAAGTTGGATTCAATACTCAATTCCACACCCTCATTTGTCTCCTCTGCATCTCAAATAGCTGATGGGGTTCGGTTGCTGCTTTCCCAGACAAGCAGCAATGACATAGTCGTTGGTGTTTATGATAGCGAACCCACTAGCATAATCTCATATGCCCTTAGTTCCAAGGATTATGAGGACTGGGTTGCTGATAATTTGAACGAGCATCAGGCAGGCTGGAGTATTCATGAAAGCTTTAAAGAAGATTCTGCAGCTTCTATCTTTTCACCCTGGCAGTCTTTTGGCTCTATGGACTTGGATTACATCCATTATGGAAATTATGGATCTGAAGATGCTTCAGGTTCCATGGGCAACCTGTTTGCAGATGCCAAAAGATCTCCACATTTGAGGATATCTTTCGGGGACGAGTCCTCAAATGGGGTAGGAAAAGTGAGATTTTCTGTGACTTGTTATTTTGCAAAGCAGTTTGACTCTTTTAGGAAAAAGTGCTGCCCCAGTGAAGTGGATTTTGTACGATCTTTGAGCCGCTGCCAGAGGTGGAGTGCACAAGGGGGAAAGAGCAATGTATATTTTGCTAAATCTCTGGATGATAGATTCATCATAAAACAAGTCACAAAGACGGAATTGGAATCCTTTCAGGAATATGCACCTGAGTATTTTAATTATTTGACAGATTCCCTTAACTCAGGAAGTCCAACTTGTCTTGCCAAGGTTCTTGGTATGTATCAGGTCACTGTTAAACAGCTGAAAGGTGGGAAGGAAACTAAAATGGATTTGATGGTGATGGAGAACCTTTTCTTCAAAAGAAACATTTCAAGGGTATATGATCTCAAGGGGTCTACTCGATCCCGTTATAATTCTGATACAACAGGGGTAGACAAGGTACTGTTAGATATGAATTTGTTGGAATCATTACGTACACAACCCATATTTCTTGGAAGCAAAGCAAAGAGAAGCCTGGAGAGAGCTGTTTGGAATGATACGTCCTTTTTAGCGTCTGTTGATGTAATGGACTACTCGCTGCTCGTCGGGGTGGACGATGAGCGGAAGGAGCTGGTTTTGGGGATCATAGATTTCATGAGACAATATACATGGGACAAGCATTTGGAGACATGGGTAAAGGCATCCGGAATACTTGGAGGCCCGAAGAATGCTGCTCCGACCATTATTTCCCCGAAGCAATACAAGAAACGATTCAGGAAGGCGATGACTGCTTATTTTCTCACCGTGCCTGATCAATGGTCGTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0008150 GO:0008152 GO:0008289 GO:0009987 GO:0010008 GO:0010118 GO:0012505 GO:0016020 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0090332 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 PIP5K

Protein Analysis

1716

Amino Acids

191.93

Weight (kDa)

5.72

Isoelectric Point (pI)

49.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cpn60_TCP1	PF00118	362 - 612	4.5e-33	TCP-1/cpn60 chaperonin family
PIP5K	PF01504	1482 - 1649	4.3e-36	Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K	PF01504	1646 - 1703	2.9e-07	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006044)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71010
fragaria_vesca	FvH4_4g32320 FvH4_4g32320 FvH4_4g32320 FvH4_4g32320 FvH4_4g32320
malus_domestica	MD13G1026200.v1.1 MD16G1029100.v1.1
prunus_persica	Prupe.1G323700_v2.0.a1 Prupe.1G323700_v2.0.a1
pyrus_communis	pycom13g02450 pycom13g02470 pycom16g02480 pycom16g02500
rosa_chinensis	RchiOBHm_Chr4g0441111
rosa_laevigata	RLG00000006113
rosa_multiflora	Rmu_sc0006317.1_g000017 Rmu_sc0027558.1_g000002
rosa_roxburghii	Rroxscaffold_5G00382080
rosa_rugosa	Rorug04G0331100 Rorug04G0331200
rosa_samantha	Rh4AG382800 Rh4BG396600 Rh4CG411700 Rh4DG389400 Rh6CG153000
rosa_wichuraiana	Rw4G033040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1241, 4752
AarI	CACCTGC	1 cut(s)	3309
AasI	GACNNNNNNGTC	1 cut(s)	2512
Acc16I	TGCGCA	1 cut(s)	2411
Acc36I	ACCTGC	3 cut(s)	1494, 3124, 3309
AccB1I	GGYRCC	1 cut(s)	2622
AccB7I	CCANNNNNTGG	1 cut(s)	3058
AccBSI	CCGCTC	1 cut(s)	4942
AccI	GTMKAC	4 cut(s)	3159, 3794, 4736, 4773
AccII	CGCG	3 cut(s)	2846, 3815, 3891
AccIII	TCCGGA	1 cut(s)	5024
AciI	CCGC	5 cut(s)	802, 848, 2844, 4423, 4942
AcoI	YGGCCR	4 cut(s)	803, 2773, 2896, 3352
AcsI	RAATTY	9 cut(s)	600, 1138, 1344, 1635, 1915, 2417, 2888, 3383, 4795
AcuI	CTGAAG	7 cut(s)	1760, 2021, 2862, 3474, 3660, 4218, 4245
AcyI	GRCGYC	2 cut(s)	1717, 2569
AfiI	CCNNNNNNNGG	4 cut(s)	722, 3058, 3693, 4314
AflII	CTTAAG	1 cut(s)	3077
AflIII	ACRYGT	2 cut(s)	2669, 3813
AgeI	ACCGGT	2 cut(s)	29, 2625
AhlI	ACTAGT	1 cut(s)	3668
AjnI	CCWGG	8 cut(s)	1180, 1953, 2590, 2696, 3139, 3876, 4166, 4854
Alw21I	GWGCWC	4 cut(s)	1039, 1330, 1564, 4444
Alw44I	GTGCAC	2 cut(s)	1560, 4440
AlwNI	CAGNNNCTG	1 cut(s)	2693
Aor13HI	TCCGGA	1 cut(s)	5024
AoxI	GGCC	7 cut(s)	699, 713, 803, 2773, 2896, 3352, 5038
ApaLI	GTGCAC	2 cut(s)	1560, 4440
ApoI	RAATTY	9 cut(s)	600, 1138, 1344, 1635, 1915, 2417, 2888, 3383, 4795
AseI	ATTAAT	1 cut(s)	2559
AsiGI	ACCGGT	2 cut(s)	29, 2625
Asp700I	GAANNNNTTC	6 cut(s)	309, 3571, 4530, 4691, 4708, 5090
AspLEI	GCGC	2 cut(s)	1281, 2412
AspS9I	GGNCC	2 cut(s)	677, 5039
AsuC2I	CCSGG	2 cut(s)	2034, 3399
AsuII	TTCGAA	1 cut(s)	1701
AvaII	GGWCC	1 cut(s)	677
BaeGI	GKGCMC	3 cut(s)	1564, 2644, 4444
BaeI	ACNNNNGTAYC	2 cut(s)	1319, 1352
BalI	TGGCCA	3 cut(s)	2775, 2898, 3354
BamHI	GGATCC	3 cut(s)	572, 1177, 2144
BanI	GGYRCC	1 cut(s)	2622
BanII	GRGCYC	2 cut(s)	98, 1039
BauI	CACGAG	1 cut(s)	3651
BbsI	GAAGAC	3 cut(s)	2789, 3369, 3462
Bbv12I	GWGCWC	4 cut(s)	1039, 1330, 1564, 4444
BceAI	ACGGC	1 cut(s)	686
BciT130I	CCWGG	8 cut(s)	1182, 1955, 2592, 2698, 3141, 3878, 4168, 4856
BciVI	GTATCC	3 cut(s)	849, 2362, 3472
BclI	TGATCA	5 cut(s)	319, 2290, 2473, 3592, 5131
BcnI	CCSGG	2 cut(s)	2034, 3399
BcuI	ACTAGT	1 cut(s)	3668
BfaI	CTAG	8 cut(s)	42, 540, 597, 615, 1305, 3669, 3855, 4035
BfmI	CTRYAG	1 cut(s)	4146
BfrI	CTTAAG	1 cut(s)	3077
BfuAI	ACCTGC	3 cut(s)	1494, 3124, 3309
BfuI	GTATCC	3 cut(s)	849, 2362, 3472
BglII	AGATCT	3 cut(s)	3008, 3070, 4271
BmcAI	AGTACT	3 cut(s)	142, 152, 2315
Bme18I	GGWCC	1 cut(s)	677
BmgT120I	GGNCC	2 cut(s)	677, 5039
BmiI	GGNNCC	7 cut(s)	574, 679, 1002, 1179, 2146, 2624, 4240
BmrI	ACTGGG	6 cut(s)	578, 1874, 2453, 3594, 4087, 4386
BmuI	ACTGGG	6 cut(s)	578, 1874, 2453, 3594, 4087, 4386
BoxI	GACNNNNGTC	1 cut(s)	2247
BpiI	GAAGAC	3 cut(s)	2789, 3369, 3462
BplI	GAGNNNNNCTC	4 cut(s)	2303, 2335, 3175, 3207
BpmI	CTGGAG	6 cut(s)	1556, 2266, 3188, 3840, 4135, 4877
Bpu14I	TTCGAA	1 cut(s)	1701
BpuEI	CTTGAG	4 cut(s)	1352, 1461, 3202, 4712
BpuMI	CCSGG	2 cut(s)	2034, 3399
BsaAI	YACGTR	2 cut(s)	2672, 4814
BsaBI	GATNNNNATC	2 cut(s)	1409, 2295
BsaHI	GRCGYC	2 cut(s)	1717, 2569
BsaI	GGTCTC	1 cut(s)	833
BsaWI	WCCGGW	5 cut(s)	29, 923, 1623, 2625, 5024
Bsc4I	CCNNNNNNNGG	4 cut(s)	722, 3058, 3693, 4314
Bse118I	RCCGGY	2 cut(s)	29, 2625
Bse3DI	GCAATG	6 cut(s)	127, 3409, 3782, 3881, 4003, 4465
Bse8I	GATNNNNATC	2 cut(s)	1409, 2295
BseAI	TCCGGA	1 cut(s)	5024
BseBI	CCWGG	8 cut(s)	1182, 1955, 2592, 2698, 3141, 3878, 4168, 4856
BseJI	GATNNNNATC	2 cut(s)	1409, 2295
BseLI	CCNNNNNNNGG	4 cut(s)	722, 3058, 3693, 4314
BseMI	GCAATG	6 cut(s)	127, 3409, 3782, 3881, 4003, 4465
BseMII	CTCAG	9 cut(s)	90, 680, 708, 1862, 2720, 3363, 3552, 4539, 4596
BseRI	GAGGAG	5 cut(s)	391, 464, 1179, 3011, 3931
BseSI	GKGCMC	3 cut(s)	1564, 2644, 4444
BsgI	GTGCAG	1 cut(s)	1711
Bsh1236I	CGCG	3 cut(s)	2846, 3815, 3891
BshFI	GGCC	7 cut(s)	701, 715, 805, 2775, 2898, 3354, 5040
BshNI	GGYRCC	1 cut(s)	2622
BshTI	ACCGGT	2 cut(s)	29, 2625
BsiHKAI	GWGCWC	4 cut(s)	1039, 1330, 1564, 4444
BsiSI	CCGG	8 cut(s)	30, 924, 1624, 2006, 2034, 2626, 3399, 5025
BslFI	GGGAC	4 cut(s)	663, 3601, 4313, 5006
BslI	CCNNNNNNNGG	4 cut(s)	722, 3058, 3693, 4314
BsmBI	CGTCTC	2 cut(s)	3178, 3474
BsmFI	GGGAC	4 cut(s)	663, 3601, 4313, 5006
BsmI	GAATGC	3 cut(s)	628, 3493, 5053
BsnI	GGCC	7 cut(s)	701, 715, 805, 2775, 2898, 3354, 5040
Bso31I	GGTCTC	1 cut(s)	833
Bsp119I	TTCGAA	1 cut(s)	1701
Bsp1286I	GDGCHC	6 cut(s)	98, 1039, 1330, 1564, 2644, 4444
Bsp13I	TCCGGA	1 cut(s)	5024
Bsp1407I	TGTACA	2 cut(s)	634, 1256
Bsp19I	CCATGG	2 cut(s)	3267, 4242
BspACI	CCGC	5 cut(s)	802, 848, 2844, 4423, 4942
BspANI	GGCC	7 cut(s)	701, 715, 805, 2775, 2898, 3354, 5040
BspCNI	CTCAG	9 cut(s)	91, 679, 709, 1863, 2721, 3364, 3553, 4540, 4595
BspEI	TCCGGA	1 cut(s)	5024
BspFNI	CGCG	3 cut(s)	2846, 3815, 3891
BspHI	TCATGA	4 cut(s)	2239, 4123, 4974, 5144
BspLI	GGNNCC	7 cut(s)	574, 679, 1002, 1179, 2146, 2624, 4240
BspMAI	CTGCAG	1 cut(s)	4150
BspMI	ACCTGC	3 cut(s)	1494, 3124, 3309
BspQI	GCTCTTC	1 cut(s)	1434
BspT104I	TTCGAA	1 cut(s)	1701
BspT107I	GGYRCC	1 cut(s)	2622
BspTI	CTTAAG	1 cut(s)	3077
BspTNI	GGTCTC	1 cut(s)	833
BsrBI	CCGCTC	1 cut(s)	4942
BsrDI	GCAATG	6 cut(s)	127, 3409, 3782, 3881, 4003, 4465
BsrFI	RCCGGY	2 cut(s)	29, 2625
BsrGI	TGTACA	2 cut(s)	634, 1256
BssAI	RCCGGY	2 cut(s)	29, 2625
BssNI	GRCGYC	2 cut(s)	1717, 2569
BssSI	CACGAG	1 cut(s)	3651
BssT1I	CCWWGG	5 cut(s)	1712, 3267, 4062, 4242, 4605
Bst2BI	CACGAG	1 cut(s)	3651
Bst2UI	CCWGG	8 cut(s)	1182, 1955, 2592, 2698, 3141, 3878, 4168, 4856
Bst6I	CTCTTC	3 cut(s)	441, 1434, 2326
BstACI	GRCGYC	2 cut(s)	1717, 2569
BstAFI	CTTAAG	1 cut(s)	3077
BstAPI	GCANNNNNTGC	1 cut(s)	1799
BstAUI	TGTACA	2 cut(s)	634, 1256
BstBAI	YACGTR	2 cut(s)	2672, 4814
BstBI	TTCGAA	1 cut(s)	1701
BstC8I	GCNNGC	5 cut(s)	1131, 1656, 2234, 3224, 4111
BstDSI	CCRYGG	2 cut(s)	3267, 4242
BstENI	CCTNNNNNAGG	1 cut(s)	720
BstFNI	CGCG	3 cut(s)	2846, 3815, 3891
BstHHI	GCGC	2 cut(s)	1281, 2412
BstMWI	GCNNNNNNNGC	9 cut(s)	117, 957, 1130, 1167, 1615, 1799, 3706, 4948, 5108
BstNI	CCWGG	8 cut(s)	1182, 1955, 2592, 2698, 3141, 3878, 4168, 4856
BstNSI	RCATGY	2 cut(s)	964, 1781
BstPAI	GACNNNNGTC	1 cut(s)	2247
BstSFI	CTRYAG	1 cut(s)	4146
BstSLI	GKGCMC	3 cut(s)	1564, 2644, 4444
BstSNI	TACGTA	1 cut(s)	4814
BstUI	CGCG	3 cut(s)	2846, 3815, 3891
BstV2I	GAAGAC	3 cut(s)	2789, 3369, 3462
BstX2I	RGATCY	9 cut(s)	572, 733, 1177, 2144, 3008, 3070, 3844, 4220, 4271
BstXI	CCANNNNNNTGG	3 cut(s)	2651, 3818, 4399
BstYI	RGATCY	9 cut(s)	572, 733, 1177, 2144, 3008, 3070, 3844, 4220, 4271
BsuI	GTATCC	3 cut(s)	849, 2362, 3472
BsuRI	GGCC	7 cut(s)	701, 715, 805, 2775, 2898, 3354, 5040
BtgI	CCRYGG	2 cut(s)	3267, 4242
BtgZI	GCGATG	1 cut(s)	5117
BtsI	GCAGTG	2 cut(s)	1800, 3242
BveI	ACCTGC	3 cut(s)	1494, 3124, 3309
Cac8I	GCNNGC	5 cut(s)	1131, 1656, 2234, 3224, 4111
CaiI	CAGNNNCTG	1 cut(s)	2693
CciI	TCATGA	4 cut(s)	2239, 4123, 4974, 5144
CfoI	GCGC	2 cut(s)	1281, 2412
Cfr10I	RCCGGY	2 cut(s)	29, 2625
Cfr13I	GGNCC	2 cut(s)	677, 5039
CseI	GACGC	5 cut(s)	1706, 2577, 3804, 3805, 4881
CspAI	ACCGGT	2 cut(s)	29, 2625
CspCI	CAANNNNNGTGG	4 cut(s)	16, 51, 2894, 2929
DraI	TTTAAA	1 cut(s)	4135
DrdI	GACNNNNNNGTC	1 cut(s)	2512
DseDI	GACNNNNNNGTC	1 cut(s)	2512
EaeI	YGGCCR	4 cut(s)	803, 2773, 2896, 3352
Eam1104I	CTCTTC	3 cut(s)	441, 1434, 2326
EarI	CTCTTC	3 cut(s)	441, 1434, 2326
EciI	GGCGGA	1 cut(s)	837
Ecl136II	GAGCTC	1 cut(s)	1037
Eco105I	TACGTA	1 cut(s)	4814
Eco130I	CCWWGG	5 cut(s)	1712, 3267, 4062, 4242, 4605
Eco147I	AGGCCT	1 cut(s)	715
Eco24I	GRGCYC	2 cut(s)	98, 1039
Eco31I	GGTCTC	1 cut(s)	833
Eco32I	GATATC	5 cut(s)	47, 1588, 2453, 2973, 4290
Eco47I	GGWCC	1 cut(s)	677
Eco53kI	GAGCTC	1 cut(s)	1037
Eco57I	CTGAAG	7 cut(s)	1760, 2021, 2862, 3474, 3660, 4218, 4245
EcoICRI	GAGCTC	1 cut(s)	1037
EcoNI	CCTNNNNNAGG	1 cut(s)	720
EcoRI	GAATTC	2 cut(s)	1915, 2888
EcoRII	CCWGG	8 cut(s)	1180, 1953, 2590, 2696, 3139, 3876, 4166, 4854
EcoRV	GATATC	5 cut(s)	47, 1588, 2453, 2973, 4290
EcoT14I	CCWWGG	5 cut(s)	1712, 3267, 4062, 4242, 4605
EcoT22I	ATGCAT	1 cut(s)	3495
EcoT38I	GRGCYC	2 cut(s)	98, 1039
ErhI	CCWWGG	5 cut(s)	1712, 3267, 4062, 4242, 4605
Esp3I	CGTCTC	2 cut(s)	3178, 3474
FalI	AAGNNNNNCTT	8 cut(s)	1450, 1482, 2535, 2567, 3060, 3092, 4579, 4611
FaqI	GGGAC	4 cut(s)	663, 3601, 4313, 5006
FauNDI	CATATG	1 cut(s)	4048
FbaI	TGATCA	5 cut(s)	319, 2290, 2473, 3592, 5131
FblI	GTMKAC	4 cut(s)	3159, 3794, 4736, 4773
FriOI	GRGCYC	2 cut(s)	98, 1039
FspBI	CTAG	8 cut(s)	42, 540, 597, 615, 1305, 3669, 3855, 4035
FspI	TGCGCA	1 cut(s)	2411
GlaI	GCGC	2 cut(s)	1280, 2411
GsuI	CTGGAG	6 cut(s)	1556, 2266, 3188, 3840, 4135, 4877
HaeIII	GGCC	7 cut(s)	701, 715, 805, 2775, 2898, 3354, 5040
HapII	CCGG	8 cut(s)	30, 924, 1624, 2006, 2034, 2626, 3399, 5025
HgaI	GACGC	5 cut(s)	1706, 2577, 3804, 3805, 4881
HhaI	GCGC	2 cut(s)	1281, 2412
Hin1I	GRCGYC	2 cut(s)	1717, 2569
Hin6I	GCGC	2 cut(s)	1279, 2410
HinP1I	GCGC	2 cut(s)	1279, 2410
HincII	GTYRAC	2 cut(s)	689, 3795
HindII	GTYRAC	2 cut(s)	689, 3795
HindIII	AAGCTT	2 cut(s)	2230, 4129
HpaII	CCGG	8 cut(s)	30, 924, 1624, 2006, 2034, 2626, 3399, 5025
Hpy99I	CGWCG	3 cut(s)	3488, 3799, 4928
HpyCH4IV	ACGT	6 cut(s)	2671, 3162, 3187, 3459, 4813, 4880
HpyF10VI	GCNNNNNNNGC	9 cut(s)	117, 957, 1130, 1167, 1615, 1799, 3706, 4948, 5108
HpySE526I	ACGT	6 cut(s)	2671, 3162, 3187, 3459, 4813, 4880
Hsp92I	GRCGYC	2 cut(s)	1717, 2569
HspAI	GCGC	2 cut(s)	1279, 2410
Kpn2I	TCCGGA	1 cut(s)	5024
Ksp22I	TGATCA	5 cut(s)	319, 2290, 2473, 3592, 5131
LguI	GCTCTTC	1 cut(s)	1434
LmnI	GCTCC	8 cut(s)	726, 1325, 1537, 2402, 3061, 3169, 4948, 5059
MaeI	CTAG	8 cut(s)	42, 540, 597, 615, 1305, 3669, 3855, 4035
MaeII	ACGT	6 cut(s)	2671, 3162, 3187, 3459, 4813, 4880
MbiI	CCGCTC	1 cut(s)	4942
MfeI	CAATTG	3 cut(s)	1119, 1578, 3036
MflI	RGATCY	9 cut(s)	572, 733, 1177, 2144, 3008, 3070, 3844, 4220, 4271
MhlI	GDGCHC	6 cut(s)	98, 1039, 1330, 1564, 2644, 4444
MlsI	TGGCCA	3 cut(s)	2775, 2898, 3354
MluI	ACGCGT	1 cut(s)	3813
MluNI	TGGCCA	3 cut(s)	2775, 2898, 3354
MmeI	TCCRAC	8 cut(s)	112, 2068, 2471, 2929, 3885, 4616, 4782, 5081
Mox20I	TGGCCA	3 cut(s)	2775, 2898, 3354
Mph1103I	ATGCAT	1 cut(s)	3495
MroI	TCCGGA	1 cut(s)	5024
MroXI	GAANNNNTTC	6 cut(s)	309, 3571, 4530, 4691, 4708, 5090
MscI	TGGCCA	3 cut(s)	2775, 2898, 3354
MslI	CAYNNNNRTG	2 cut(s)	1437, 2649
Msp20I	TGGCCA	3 cut(s)	2775, 2898, 3354
MspA1I	CMGCKG	4 cut(s)	802, 1907, 4425, 4642
MspCI	CTTAAG	1 cut(s)	3077
MspI	CCGG	8 cut(s)	30, 924, 1624, 2006, 2034, 2626, 3399, 5025
MunI	CAATTG	3 cut(s)	1119, 1578, 3036
Mva1269I	GAATGC	3 cut(s)	628, 3493, 5053
MvaI	CCWGG	8 cut(s)	1182, 1955, 2592, 2698, 3141, 3878, 4168, 4856
MvnI	CGCG	3 cut(s)	2846, 3815, 3891
MwoI	GCNNNNNNNGC	9 cut(s)	117, 957, 1130, 1167, 1615, 1799, 3706, 4948, 5108
NciI	CCSGG	2 cut(s)	2034, 3399
NcoI	CCATGG	2 cut(s)	3267, 4242
NdeI	CATATG	1 cut(s)	4048
NlaIV	GGNNCC	7 cut(s)	574, 679, 1002, 1179, 2146, 2624, 4240
NmeAIII	GCCGAG	2 cut(s)	2536, 3503
NmuCI	GTSAC	8 cut(s)	440, 564, 795, 1226, 2075, 4339, 4507, 4627
NsbI	TGCGCA	1 cut(s)	2411
NsiI	ATGCAT	1 cut(s)	3495
NspI	RCATGY	2 cut(s)	964, 1781
NspV	TTCGAA	1 cut(s)	1701
PagI	TCATGA	4 cut(s)	2239, 4123, 4974, 5144
PaqCI	CACCTGC	1 cut(s)	3309
PceI	AGGCCT	1 cut(s)	715
PciSI	GCTCTTC	1 cut(s)	1434
PcsI	WCGNNNNNNNCGW	2 cut(s)	3184, 4932
PctI	GAATGC	3 cut(s)	628, 3493, 5053
PdmI	GAANNNNTTC	6 cut(s)	309, 3571, 4530, 4691, 4708, 5090
PflFI	GACNNNGTC	1 cut(s)	4004
PflMI	CCANNNNNTGG	1 cut(s)	3058
PinAI	ACCGGT	2 cut(s)	29, 2625
Ppu21I	YACGTR	2 cut(s)	2672, 4814
PshAI	GACNNNNGTC	1 cut(s)	2247
PshBI	ATTAAT	1 cut(s)	2559
PsiI	TTATAA	2 cut(s)	1241, 4752
Psp124BI	GAGCTC	1 cut(s)	1039
Psp6I	CCWGG	8 cut(s)	1180, 1953, 2590, 2696, 3139, 3876, 4166, 4854
PspGI	CCWGG	8 cut(s)	1180, 1953, 2590, 2696, 3139, 3876, 4166, 4854
PspN4I	GGNNCC	7 cut(s)	574, 679, 1002, 1179, 2146, 2624, 4240
PspPI	GGNCC	2 cut(s)	677, 5039
PsrI	GAACNNNNNNTAC	4 cut(s)	520, 552, 3417, 3449
PstI	CTGCAG	1 cut(s)	4150
PstNI	CAGNNNCTG	1 cut(s)	2693
PsuI	RGATCY	9 cut(s)	572, 733, 1177, 2144, 3008, 3070, 3844, 4220, 4271
PsyI	GACNNNGTC	1 cut(s)	4004
PvuII	CAGCTG	2 cut(s)	1907, 4642
RseI	CAYNNNNRTG	2 cut(s)	1437, 2649
SacI	GAGCTC	1 cut(s)	1039
SalI	GTCGAC	1 cut(s)	3793
SapI	GCTCTTC	1 cut(s)	1434
Sau96I	GGNCC	2 cut(s)	677, 5039
ScaI	AGTACT	3 cut(s)	142, 152, 2315
SduI	GDGCHC	6 cut(s)	98, 1039, 1330, 1564, 2644, 4444
SfcI	CTRYAG	1 cut(s)	4146
SfuI	TTCGAA	1 cut(s)	1701
SgrAI	CRCCGGYG	1 cut(s)	2625
SinI	GGWCC	1 cut(s)	677
SmiMI	CAYNNNNRTG	2 cut(s)	1437, 2649
SmlI	CTYRAG	5 cut(s)	1331, 1476, 3077, 3181, 4727
SmoI	CTYRAG	5 cut(s)	1331, 1476, 3077, 3181, 4727
SnaBI	TACGTA	1 cut(s)	4814
SpeI	ACTAGT	1 cut(s)	3668
SseBI	AGGCCT	1 cut(s)	715
SsiI	CCGC	5 cut(s)	802, 848, 2844, 4423, 4942
SspI	AATATT	2 cut(s)	497, 1990
SspMI	CTAG	8 cut(s)	42, 540, 597, 615, 1305, 3669, 3855, 4035
SstI	GAGCTC	1 cut(s)	1039
StuI	AGGCCT	1 cut(s)	715
StyI	CCWWGG	5 cut(s)	1712, 3267, 4062, 4242, 4605
TaiI	ACGT	6 cut(s)	2674, 3165, 3190, 3462, 4816, 4883
TaqI	TCGA	6 cut(s)	956, 1368, 1701, 2985, 3794, 4741
TatI	WGTACW	9 cut(s)	140, 150, 634, 1256, 1293, 1553, 2313, 2371, 2980
TauI	GCSGC	2 cut(s)	805, 4425
TseFI	GTSAC	8 cut(s)	440, 564, 795, 1226, 2075, 4339, 4507, 4627
Tsp45I	GTSAC	8 cut(s)	440, 564, 795, 1226, 2075, 4339, 4507, 4627
TspGWI	ACGGA	3 cut(s)	210, 767, 4532
Tth111I	GACNNNGTC	1 cut(s)	4004
Van91I	CCANNNNNTGG	1 cut(s)	3058
Vha464I	CTTAAG	1 cut(s)	3077
VneI	GTGCAC	2 cut(s)	1560, 4440
VpaK11BI	GGWCC	1 cut(s)	677
VspI	ATTAAT	1 cut(s)	2559
XagI	CCTNNNNNAGG	1 cut(s)	720
XapI	RAATTY	9 cut(s)	600, 1138, 1344, 1635, 1915, 2417, 2888, 3383, 4795
XbaI	TCTAGA	3 cut(s)	41, 539, 596
XceI	RCATGY	2 cut(s)	964, 1781
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1444
XmiI	GTMKAC	4 cut(s)	3159, 3794, 4736, 4773
XmnI	GAANNNNTTC	6 cut(s)	309, 3571, 4530, 4691, 4708, 5090
XspI	CTAG	8 cut(s)	42, 540, 597, 615, 1305, 3669, 3855, 4035
ZrmI	AGTACT	3 cut(s)	142, 152, 2315
Zsp2I	ATGCAT	1 cut(s)	3495

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.