Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0006044

Physical Location & Seq

Genomic Coordinates Forward (+)

50553226 .. 50557551

Gene Structure 4326 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0331200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2160 bp

ATGGGGTCTTCACGGTTGTTCAAGAGGTTTAGCGGCCTCTACAAACCCAATGCCAGAATCCAACCATACCCATCTGCCTACGTCCCCACAAGAGCCCTATCACACTCCAAGCTTTTCAGCAATGGCGACAATTCTGTGCTTCCGGTTCTGATAGTCGGTGCAGGACCTGTGGGTCTCGTTCTTTCTATACTCCTAACAAAGCTAGGGGTCAAATGCGCGGTTTTGGAGAAAAGTACAAGTTTCTCCAGACATCCACAGGCACACTTCATTAACAATCGGTCGATGGAGGTATTTCGCAAATTGGATGGCCTGGCAGAGGAGATCCAGAAGTCTCAACCACCAGTTGATTTGTGGAGAAAGTTTATATATTGCACTTCGCTCTCTGGTTCCATTCTTGGCTCAGTAGATCATATGCAACCTGAAGATTTTGAGCAAGTTGTCAGCCCGGTCTCTGTTGCACACTTCTCTCAGTACAAACTGAATGCATTACTACTTAAGCAGCTTGAAAATCTCAGCTTCAAGTATTGTACCCCTGAAGGGTTGAAAGGCCTTGACCATGAACCCCTTGAGGCAAGGCAAATATTGATGGGCCATGAGTGTGTTTCCATCAATGCCAGTGATGATATTATTTCCGTGACTGCATCTTTTCTCAAGGATGGGAAACATATGGAGAGGAATATCAGATGCAATATGATTGTAGGTACAGATGGTGCTGGAAGTACTGTACGTAAGCTGGCTGGTGTAGATATGAGAGGTGAAAAGGACTTGCAGAAGCTTGTGAGTGTCCATTTCTTGAGTAGAGACCTTGGAAATTACTTGCTCAGTGAGAGACCTGGCATGCTGTTTTTTATCTTCAATACTGAAGCTATAGGGGTCCTTGTTGCACATGATCTCAAGCAAGGGGAATTTGTATTGCAGATACCATTTTATCCACCCCAGCAAAACCTTGAAGATTTCAGTGCCAAGACATGTGAGAGGATGATCTTCAAATTGGTAGGTCGAGAGCTTGGAGACATAAATGTGATTGACATAAAACCTTGGGTGATGCATGCTGAAGTTGCCGAGAAGTTTCTAAGTAGTCGAAACCGTGTGATTCTTGCTGGTGATGCTGCTCATCAATTCCCTCCTGCTGGTGGATTTGGTATGAATACCGGAATTCAGGATTCTCATAACCTTGCCTGGAAGATAGCTTCTGTAGTAAAGGGTATTACACCACCTTCAATACTTAATACTTATGAAACAGAACGTAGGCCGATTGCCATTTTCAATACAACTCTTAGTATTCAGAACTTCAAAGCAGCCATGGCTGTTCCTGGTGCACTTGGTCTTGATCCAACTATTGCAAACTCGGTACATCGAGTAATAAATAAGGGGATTGGGTCTATTTTACCATCTGGACTACAGAGCGCCATTTTGGATGGAATTTTCACCTTAGGTCGTGCACAGTTATCAGAAACTCTTTTGAATGAAAACAACCCACTTGGAGCTTTAAGGCTTGCTAATCTGAGACGCATATTTGAAGAAGGAAAGAGTCTCCAGCTCCAGTTCCCGGCAGAAGATCTTGGTTTCAGGTACCTAGAAGGCGCACTTGTTCCTGACCATGATGGTGCACTGAGTGGCCCAGATGGACCTACTGGTCGCCGGAGGGACTATGTTCCTACTGCAGATCCAGGTTCAAGGCTTCCTCACATGAATGTGAGGATATTGTCAAATTCATCGAGTGAGGCAACTATTTCTACACTGGATCTTATATCTGGAGACAAAATTGAGTTTCTCCTTATCATAGCACCGATAGAGCCATCCTACCATCTTGCTCATGCTGCATTGAAGGTGGCTGAGGAATTCAAAGTTTCTGCAAGGGTGTGCGTGTTGTGGCCTGCTGGTAGTGCTAAAGGAGTTGAGGTAAGAAGTAAGGCATCATTGAAACCGTGGGAGAATTACATAGATGTTATGGAAGTTAAAAAGTCATCAGATGCTTTGTCATGGTGGGACATCTGTCAAATGACTGATAAAGGAGCCATCCTAGTTCGGCCTGATGAGCATGTTGCTTGGCGTCTGAAGTCTGGAGTTGTTGGGGACCCTATGAGGGAAATGAGAAGGGCTTTTTCTGCCGCCCTAGGCTTGAAGTCACATATTAAAGACCAAGAAGCTGATCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0008150 GO:0008152 GO:0008289 GO:0009987 GO:0010008 GO:0010118 GO:0012505 GO:0016020 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0090332 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 PIP5K

Protein Analysis

719

Amino Acids

79.12

Weight (kDa)

8.01

Isoelectric Point (pI)

36.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_3	PF01494	48 - 420	3.4e-68	FAD binding domain
Rng_hyd_C	PF21274	637 - 705	5.5e-07	Aromatic-ring hydroxylase, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006044)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71010
fragaria_vesca	FvH4_4g32320 FvH4_4g32320 FvH4_4g32320 FvH4_4g32320 FvH4_4g32320
malus_domestica	MD13G1026200.v1.1 MD16G1029100.v1.1
prunus_persica	Prupe.1G323700_v2.0.a1 Prupe.1G323700_v2.0.a1
pyrus_communis	pycom13g02450 pycom13g02470 pycom16g02480 pycom16g02500
rosa_chinensis	RchiOBHm_Chr4g0441111
rosa_laevigata	RLG00000006113
rosa_multiflora	Rmu_sc0006317.1_g000017 Rmu_sc0027558.1_g000002
rosa_roxburghii	Rroxscaffold_5G00382080
rosa_rugosa	Rorug04G0331100 Rorug04G0331200
rosa_samantha	Rh4AG382800 Rh4BG396600 Rh4CG411700 Rh4DG389400 Rh6CG153000
rosa_wichuraiana	Rw4G033040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	171, 1635
Acc65I	GGTACC	1 cut(s)	1572
AccB1I	GGYRCC	1 cut(s)	1572
AccII	CGCG	1 cut(s)	218
AciI	CCGC	3 cut(s)	33, 218, 2112
AclWI	GGATC	4 cut(s)	316, 1325, 1661, 1752
AcsI	RAATTY	5 cut(s)	905, 1155, 1422, 1711, 1841
AcuI	CTGAAG	5 cut(s)	441, 555, 882, 1074, 2078
AcyI	GRCGYC	1 cut(s)	2053
AdeI	CACNNNGTG	1 cut(s)	1616
AfaI	GTAC	8 cut(s)	235, 473, 529, 703, 721, 726, 1353, 1574
AfiI	CCNNNNNNNGG	6 cut(s)	316, 537, 1130, 1133, 1549, 2086
AflII	CTTAAG	1 cut(s)	494
AflIII	ACRYGT	1 cut(s)	968
AjnI	CCWGG	5 cut(s)	309, 832, 1178, 1312, 1669
Alw21I	GWGCWC	3 cut(s)	1321, 1444, 1612
Alw26I	GTCTC	9 cut(s)	179, 336, 454, 795, 823, 1005, 1501, 1538, 1752
Alw44I	GTGCAC	3 cut(s)	1317, 1440, 1608
AlwI	GGATC	4 cut(s)	316, 1325, 1661, 1752
AlwNI	CAGNNNCTG	1 cut(s)	167
AoxI	GGCC	8 cut(s)	34, 307, 547, 589, 1250, 1618, 1874, 2030
ApaLI	GTGCAC	3 cut(s)	1317, 1440, 1608
ApeKI	GCWGC	4 cut(s)	499, 1109, 1298, 1820
ApoI	RAATTY	5 cut(s)	905, 1155, 1422, 1711, 1841
Asp718I	GGTACC	1 cut(s)	1572
AspA2I	CCTAGG	1 cut(s)	2116
AspLEI	GCGC	3 cut(s)	218, 1409, 1586
AspS9I	GGNCC	6 cut(s)	164, 589, 874, 1619, 1628, 2077
AsuC2I	CCSGG	2 cut(s)	446, 1550
AsuHPI	GGTGA	4 cut(s)	767, 1054, 1115, 1420
AvaII	GGWCC	4 cut(s)	164, 874, 1628, 2077
AvrII	CCTAGG	1 cut(s)	2116
AxyI	CCTNAGG	1 cut(s)	1432
BaeGI	GKGCMC	3 cut(s)	1321, 1444, 1612
BanI	GGYRCC	1 cut(s)	1572
BanII	GRGCYC	1 cut(s)	97
Bbv12I	GWGCWC	3 cut(s)	1321, 1444, 1612
BbvCI	CCTCAGC	1 cut(s)	1836
BbvI	GCAGC	4 cut(s)	511, 1096, 1310, 1807
BciT130I	CCWGG	5 cut(s)	311, 834, 1180, 1314, 1671
BclI	TGATCA	1 cut(s)	2152
BcnI	CCSGG	2 cut(s)	446, 1550
BcoDI	GTCTC	9 cut(s)	179, 336, 454, 795, 823, 1005, 1501, 1538, 1752
BfaI	CTAG	4 cut(s)	203, 1577, 2024, 2117
BfmI	CTRYAG	4 cut(s)	867, 1194, 1400, 1662
BfoI	RGCGCY	1 cut(s)	1410
BfrI	CTTAAG	1 cut(s)	494
BglII	AGATCT	1 cut(s)	1558
BisI	GCNGC	6 cut(s)	34, 500, 1110, 1299, 1821, 2112
BlnI	CCTAGG	1 cut(s)	2116
BlsI	GCNGC	6 cut(s)	35, 501, 1111, 1300, 1822, 2113
BmcAI	AGTACT	1 cut(s)	721
Bme1390I	CCNGG	7 cut(s)	311, 446, 834, 1180, 1314, 1550, 1671
Bme18I	GGWCC	4 cut(s)	164, 874, 1628, 2077
BmgT120I	GGNCC	6 cut(s)	164, 589, 874, 1619, 1628, 2077
BmiI	GGNNCC	6 cut(s)	388, 875, 1574, 2017, 2078, 2079
BmrFI	CCNGG	7 cut(s)	311, 446, 834, 1180, 1314, 1550, 1671
BmsI	GCATC	6 cut(s)	650, 674, 1035, 1096, 1925, 1963
BoxI	GACNNNNGTC	1 cut(s)	1995
BpmI	CTGGAG	5 cut(s)	229, 1520, 1526, 1776, 2085
Bpu10I	CCTNAGC	1 cut(s)	1836
BpuEI	CTTGAG	4 cut(s)	587, 635, 814, 878
BpuMI	CCSGG	2 cut(s)	446, 1550
BsaAI	YACGTR	1 cut(s)	728
BsaHI	GRCGYC	1 cut(s)	2053
BsaI	GGTCTC	4 cut(s)	179, 454, 795, 823
BsaJI	CCNNGG	5 cut(s)	805, 1037, 1302, 1928, 2116
BsaWI	WCCGGW	2 cut(s)	142, 1151
BsaXI	ACNNNNNCTCC	2 cut(s)	1887, 1917
Bsc4I	CCNNNNNNNGG	6 cut(s)	316, 537, 1130, 1133, 1549, 2086
Bse1I	ACTGG	5 cut(s)	341, 615, 1543, 1639, 1746
Bse21I	CCTNAGG	1 cut(s)	1432
Bse3DI	GCAATG	1 cut(s)	127
BseBI	CCWGG	5 cut(s)	311, 834, 1180, 1314, 1671
BseDI	CCNNGG	5 cut(s)	805, 1037, 1302, 1928, 2116
BseGI	GGATG	7 cut(s)	250, 310, 661, 984, 1423, 1799, 2019
BseLI	CCNNNNNNNGG	6 cut(s)	316, 537, 1130, 1133, 1549, 2086
BseMI	GCAATG	1 cut(s)	127
BseMII	CTCAG	7 cut(s)	414, 482, 526, 835, 1496, 1604, 1827
BseNI	ACTGG	5 cut(s)	341, 615, 1543, 1639, 1746
BseRI	GAGGAG	1 cut(s)	332
BseSI	GKGCMC	3 cut(s)	1321, 1444, 1612
BseXI	GCAGC	4 cut(s)	511, 1096, 1310, 1807
BseYI	CCCAGC	1 cut(s)	936
BsgI	GTGCAG	1 cut(s)	180
Bsh1236I	CGCG	1 cut(s)	218
Bsh1285I	CGRYCG	1 cut(s)	281
BshFI	GGCC	8 cut(s)	36, 309, 549, 591, 1252, 1620, 1876, 2032
BshNI	GGYRCC	1 cut(s)	1572
BsiEI	CGRYCG	1 cut(s)	281
BsiHKAI	GWGCWC	3 cut(s)	1321, 1444, 1612
BsiSI	CCGG	5 cut(s)	143, 446, 1152, 1550, 1642
BslFI	GGGAC	4 cut(s)	68, 1661, 2003, 2090
BslI	CCNNNNNNNGG	6 cut(s)	316, 537, 1130, 1133, 1549, 2086
BsmAI	GTCTC	9 cut(s)	179, 336, 454, 795, 823, 1005, 1501, 1538, 1752
BsmBI	CGTCTC	1 cut(s)	1501
BsmFI	GGGAC	4 cut(s)	68, 1661, 2003, 2090
BsmI	GAATGC	1 cut(s)	487
BsnI	GGCC	8 cut(s)	36, 309, 549, 591, 1252, 1620, 1876, 2032
Bso31I	GGTCTC	4 cut(s)	179, 454, 795, 823
Bsp1286I	GDGCHC	4 cut(s)	97, 1321, 1444, 1612
Bsp143I	GATC	9 cut(s)	321, 406, 889, 981, 1330, 1558, 1666, 1744, 2152
Bsp19I	CCATGG	1 cut(s)	1302
BspACI	CCGC	3 cut(s)	33, 218, 2112
BspANI	GGCC	8 cut(s)	36, 309, 549, 591, 1252, 1620, 1876, 2032
BspCNI	CTCAG	7 cut(s)	413, 481, 525, 834, 1497, 1605, 1828
BspFNI	CGCG	1 cut(s)	218
BspLI	GGNNCC	6 cut(s)	388, 875, 1574, 2017, 2078, 2079
BspMAI	CTGCAG	1 cut(s)	1666
BspPI	GGATC	4 cut(s)	316, 1325, 1661, 1752
BspT107I	GGYRCC	1 cut(s)	1572
BspTI	CTTAAG	1 cut(s)	494
BspTNI	GGTCTC	4 cut(s)	179, 454, 795, 823
BsrDI	GCAATG	1 cut(s)	127
BsrI	ACTGG	5 cut(s)	341, 615, 1543, 1639, 1746
BssECI	CCNNGG	5 cut(s)	805, 1037, 1302, 1928, 2116
BssMI	GATC	9 cut(s)	321, 406, 889, 981, 1330, 1558, 1666, 1744, 2152
BssNI	GRCGYC	1 cut(s)	2053
BssT1I	CCWWGG	4 cut(s)	805, 1037, 1302, 2116
Bst2UI	CCWGG	5 cut(s)	311, 834, 1180, 1314, 1671
Bst4CI	ACNGT	5 cut(s)	15, 724, 1088, 1446, 1929
BstACI	GRCGYC	1 cut(s)	2053
BstAFI	CTTAAG	1 cut(s)	494
BstBAI	YACGTR	1 cut(s)	728
BstC8I	GCNNGC	5 cut(s)	735, 839, 1050, 1497, 1878
BstDSI	CCRYGG	2 cut(s)	1302, 1928
BstENI	CCTNNNNNAGG	1 cut(s)	314
BstF5I	GGATG	7 cut(s)	250, 310, 661, 984, 1423, 1799, 2019
BstFNI	CGCG	1 cut(s)	218
BstH2I	RGCGCY	1 cut(s)	1410
BstHHI	GCGC	3 cut(s)	218, 1409, 1586
BstKTI	GATC	9 cut(s)	324, 409, 892, 984, 1333, 1561, 1669, 1747, 2155
BstMAI	GTCTC	9 cut(s)	179, 336, 454, 795, 823, 1005, 1501, 1538, 1752
BstMBI	GATC	9 cut(s)	321, 406, 889, 981, 1330, 1558, 1666, 1744, 2152
BstMCI	CGRYCG	1 cut(s)	281
BstMWI	GCNNNNNNNGC	7 cut(s)	1058, 1106, 1304, 1820, 1886, 2038, 2108
BstNI	CCWGG	5 cut(s)	311, 834, 1180, 1314, 1671
BstNSI	RCATGY	4 cut(s)	841, 972, 1052, 2045
BstPAI	GACNNNNGTC	1 cut(s)	1995
BstSCI	CCNGG	7 cut(s)	309, 444, 832, 1178, 1312, 1548, 1669
BstSFI	CTRYAG	4 cut(s)	867, 1194, 1400, 1662
BstSLI	GKGCMC	3 cut(s)	1321, 1444, 1612
BstSNI	TACGTA	1 cut(s)	728
BstUI	CGCG	1 cut(s)	218
BstV1I	GCAGC	4 cut(s)	511, 1096, 1310, 1807
BstX2I	RGATCY	4 cut(s)	321, 1558, 1666, 1744
BstYI	RGATCY	4 cut(s)	321, 1558, 1666, 1744
Bsu36I	CCTNAGG	1 cut(s)	1432
BsuRI	GGCC	8 cut(s)	36, 309, 549, 591, 1252, 1620, 1876, 2032
BtgI	CCRYGG	2 cut(s)	1302, 1928
BtsCI	GGATG	7 cut(s)	250, 310, 661, 984, 1423, 1799, 2019
BtsIMutI	CAGTG	5 cut(s)	622, 829, 964, 1610, 1739
Cac8I	GCNNGC	5 cut(s)	735, 839, 1050, 1497, 1878
CaiI	CAGNNNCTG	1 cut(s)	167
CfoI	GCGC	3 cut(s)	218, 1409, 1586
Cfr13I	GGNCC	6 cut(s)	164, 589, 874, 1619, 1628, 2077
CseI	GACGC	2 cut(s)	1518, 2042
Csp6I	GTAC	8 cut(s)	234, 472, 528, 702, 720, 725, 1352, 1573
CviQI	GTAC	8 cut(s)	234, 472, 528, 702, 720, 725, 1352, 1573
DpnI	GATC	9 cut(s)	323, 408, 891, 983, 1332, 1560, 1668, 1746, 2154
DpnII	GATC	9 cut(s)	321, 406, 889, 981, 1330, 1558, 1666, 1744, 2152
DraIII	CACNNNGTG	1 cut(s)	1616
DrdI	GACNNNNNNGTC	2 cut(s)	171, 1635
DseDI	GACNNNNNNGTC	2 cut(s)	171, 1635
Eco105I	TACGTA	1 cut(s)	728
Eco130I	CCWWGG	4 cut(s)	805, 1037, 1302, 2116
Eco147I	AGGCCT	1 cut(s)	549
Eco24I	GRGCYC	1 cut(s)	97
Eco31I	GGTCTC	4 cut(s)	179, 454, 795, 823
Eco47I	GGWCC	4 cut(s)	164, 874, 1628, 2077
Eco57I	CTGAAG	5 cut(s)	441, 555, 882, 1074, 2078
Eco81I	CCTNAGG	1 cut(s)	1432
EcoNI	CCTNNNNNAGG	1 cut(s)	314
EcoO109I	RGGNCCY	3 cut(s)	164, 874, 2077
EcoRI	GAATTC	2 cut(s)	1155, 1841
EcoRII	CCWGG	5 cut(s)	309, 832, 1178, 1312, 1669
EcoT14I	CCWWGG	4 cut(s)	805, 1037, 1302, 2116
EcoT22I	ATGCAT	2 cut(s)	487, 1050
EcoT38I	GRGCYC	1 cut(s)	97
ErhI	CCWWGG	4 cut(s)	805, 1037, 1302, 2116
Esp3I	CGTCTC	1 cut(s)	1501
FalI	AAGNNNNNCTT	2 cut(s)	1572, 1604
FaqI	GGGAC	4 cut(s)	68, 1661, 2003, 2090
FauNDI	CATATG	2 cut(s)	411, 666
FbaI	TGATCA	1 cut(s)	2152
Fnu4HI	GCNGC	6 cut(s)	34, 500, 1110, 1299, 1821, 2112
FokI	GGATG	7 cut(s)	237, 317, 668, 991, 1430, 1786, 2006
FriOI	GRGCYC	1 cut(s)	97
Fsp4HI	GCNGC	6 cut(s)	34, 500, 1110, 1299, 1821, 2112
FspBI	CTAG	4 cut(s)	203, 1577, 2024, 2117
GlaI	GCGC	3 cut(s)	217, 1408, 1585
GluI	GCNGC	6 cut(s)	34, 500, 1110, 1299, 1821, 2112
GsaI	CCCAGC	1 cut(s)	940
GsuI	CTGGAG	5 cut(s)	229, 1520, 1526, 1776, 2085
HaeII	RGCGCY	1 cut(s)	1410
HaeIII	GGCC	8 cut(s)	36, 309, 549, 591, 1252, 1620, 1876, 2032
HapII	CCGG	5 cut(s)	143, 446, 1152, 1550, 1642
HgaI	GACGC	2 cut(s)	1518, 2042
HhaI	GCGC	3 cut(s)	218, 1409, 1586
Hin1I	GRCGYC	1 cut(s)	2053
Hin6I	GCGC	3 cut(s)	216, 1407, 1584
HinP1I	GCGC	3 cut(s)	216, 1407, 1584
HindIII	AAGCTT	2 cut(s)	110, 773
HinfI	GANTC	4 cut(s)	57, 1093, 1163, 1531
HpaII	CCGG	5 cut(s)	143, 446, 1152, 1550, 1642
HphI	GGTGA	4 cut(s)	767, 1054, 1115, 1420
Hpy166II	GTNNAC	3 cut(s)	1319, 1442, 1610
Hpy188I	TCNGA	7 cut(s)	150, 683, 1287, 1453, 1506, 1972, 2058
Hpy8I	GTNNAC	3 cut(s)	1319, 1442, 1610
HpyAV	CCTTC	6 cut(s)	530, 1227, 1517, 1574, 1822, 2091
HpyCH4III	ACNGT	5 cut(s)	15, 724, 1088, 1446, 1929
HpyCH4IV	ACGT	3 cut(s)	81, 727, 1246
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1058, 1106, 1304, 1820, 1886, 2038, 2108
HpySE526I	ACGT	3 cut(s)	81, 727, 1246
Hsp92I	GRCGYC	1 cut(s)	2053
HspAI	GCGC	3 cut(s)	216, 1407, 1584
KflI	GGGWCCC	1 cut(s)	2077
KpnI	GGTACC	1 cut(s)	1576
Ksp22I	TGATCA	1 cut(s)	2152
Kzo9I	GATC	9 cut(s)	321, 406, 889, 981, 1330, 1558, 1666, 1744, 2152
LmnI	GCTCC	3 cut(s)	1484, 1545, 2015
Lsp1109I	GCAGC	4 cut(s)	511, 1096, 1310, 1807
LweI	GCATC	6 cut(s)	650, 674, 1035, 1096, 1925, 1963
MaeI	CTAG	4 cut(s)	203, 1577, 2024, 2117
MaeII	ACGT	3 cut(s)	81, 727, 1246
MaeIII	GTNAC	2 cut(s)	634, 2127
MalI	GATC	9 cut(s)	323, 408, 891, 983, 1332, 1560, 1668, 1746, 2154
MboI	GATC	9 cut(s)	321, 406, 889, 981, 1330, 1558, 1666, 1744, 2152
MboII	GAAGA	7 cut(s)	434, 844, 962, 976, 1195, 1532, 1568
MflI	RGATCY	4 cut(s)	321, 1558, 1666, 1744
MhlI	GDGCHC	4 cut(s)	97, 1321, 1444, 1612
MlyI	GAGTC	1 cut(s)	1540
MmeI	TCCRAC	2 cut(s)	85, 1358
Mph1103I	ATGCAT	2 cut(s)	487, 1050
MseI	TTAA	6 cut(s)	270, 495, 1227, 1490, 1959, 2136
MslI	CAYNNNNRTG	5 cut(s)	597, 1019, 1605, 1692, 1694
MspCI	CTTAAG	1 cut(s)	494
MspI	CCGG	5 cut(s)	143, 446, 1152, 1550, 1642
MspR9I	CCNGG	7 cut(s)	311, 446, 834, 1180, 1314, 1550, 1671
Mva1269I	GAATGC	1 cut(s)	487
MvaI	CCWGG	5 cut(s)	311, 834, 1180, 1314, 1671
MvnI	CGCG	1 cut(s)	218
MwoI	GCNNNNNNNGC	7 cut(s)	1058, 1106, 1304, 1820, 1886, 2038, 2108
NciI	CCSGG	2 cut(s)	446, 1550
NcoI	CCATGG	1 cut(s)	1302
NdeI	CATATG	2 cut(s)	411, 666
NdeII	GATC	9 cut(s)	321, 406, 889, 981, 1330, 1558, 1666, 1744, 2152
NlaIV	GGNNCC	6 cut(s)	388, 875, 1574, 2017, 2078, 2079
NmeAIII	GCCGAG	1 cut(s)	1087
NmuCI	GTSAC	2 cut(s)	634, 2127
NsiI	ATGCAT	2 cut(s)	487, 1050
NspI	RCATGY	4 cut(s)	841, 972, 1052, 2045
PaeI	GCATGC	2 cut(s)	841, 1052
PceI	AGGCCT	1 cut(s)	549
PciI	ACATGT	1 cut(s)	968
PctI	GAATGC	1 cut(s)	487
PfeI	GAWTC	3 cut(s)	57, 1093, 1163
PkrI	GCNGC	6 cut(s)	35, 501, 1111, 1300, 1822, 2113
PleI	GAGTC	1 cut(s)	1539
PpsI	GAGTC	1 cut(s)	1539
Ppu21I	YACGTR	1 cut(s)	728
PpuMI	RGGWCCY	3 cut(s)	164, 874, 2077
PscI	ACATGT	1 cut(s)	968
PshAI	GACNNNNGTC	1 cut(s)	1995
Psp5II	RGGWCCY	3 cut(s)	164, 874, 2077
Psp6I	CCWGG	5 cut(s)	309, 832, 1178, 1312, 1669
PspFI	CCCAGC	1 cut(s)	936
PspGI	CCWGG	5 cut(s)	309, 832, 1178, 1312, 1669
PspN4I	GGNNCC	6 cut(s)	388, 875, 1574, 2017, 2078, 2079
PspPI	GGNCC	6 cut(s)	164, 589, 874, 1619, 1628, 2077
PspPPI	RGGWCCY	3 cut(s)	164, 874, 2077
PstI	CTGCAG	1 cut(s)	1666
PstNI	CAGNNNCTG	1 cut(s)	167
PsuI	RGATCY	4 cut(s)	321, 1558, 1666, 1744
RsaI	GTAC	8 cut(s)	235, 473, 529, 703, 721, 726, 1353, 1574
RsaNI	GTAC	8 cut(s)	234, 472, 528, 702, 720, 725, 1352, 1573
RseI	CAYNNNNRTG	5 cut(s)	597, 1019, 1605, 1692, 1694
SaqAI	TTAA	6 cut(s)	270, 495, 1227, 1490, 1959, 2136
SatI	GCNGC	6 cut(s)	34, 500, 1110, 1299, 1821, 2112
Sau3AI	GATC	9 cut(s)	321, 406, 889, 981, 1330, 1558, 1666, 1744, 2152
Sau96I	GGNCC	6 cut(s)	164, 589, 874, 1619, 1628, 2077
ScaI	AGTACT	1 cut(s)	721
SchI	GAGTC	1 cut(s)	1540
ScrFI	CCNGG	7 cut(s)	311, 446, 834, 1180, 1314, 1550, 1671
SduI	GDGCHC	4 cut(s)	97, 1321, 1444, 1612
SfaNI	GCATC	6 cut(s)	650, 674, 1035, 1096, 1925, 1963
SfcI	CTRYAG	4 cut(s)	867, 1194, 1400, 1662
SinI	GGWCC	4 cut(s)	164, 874, 1628, 2077
SmiMI	CAYNNNNRTG	5 cut(s)	597, 1019, 1605, 1692, 1694
SmlI	CTYRAG	5 cut(s)	494, 566, 650, 793, 893
SmoI	CTYRAG	5 cut(s)	494, 566, 650, 793, 893
SnaBI	TACGTA	1 cut(s)	728
SphI	GCATGC	2 cut(s)	841, 1052
SseBI	AGGCCT	1 cut(s)	549
SsiI	CCGC	3 cut(s)	33, 218, 2112
SspI	AATATT	1 cut(s)	582
SspMI	CTAG	4 cut(s)	203, 1577, 2024, 2117
StuI	AGGCCT	1 cut(s)	549
StyD4I	CCNGG	7 cut(s)	309, 444, 832, 1178, 1312, 1548, 1669
StyI	CCWWGG	4 cut(s)	805, 1037, 1302, 2116
TaaI	ACNGT	5 cut(s)	15, 724, 1088, 1446, 1929
TaiI	ACGT	3 cut(s)	84, 730, 1249
TaqI	TCGA	5 cut(s)	281, 1000, 1081, 1357, 1718
TaqII	GACCGA	1 cut(s)	267
TatI	WGTACW	3 cut(s)	233, 471, 719
TauI	GCSGC	2 cut(s)	36, 2114
TfiI	GAWTC	3 cut(s)	57, 1093, 1163
Tru1I	TTAA	6 cut(s)	270, 495, 1227, 1490, 1959, 2136
Tru9I	TTAA	6 cut(s)	270, 495, 1227, 1490, 1959, 2136
TscAI	CASTG	5 cut(s)	622, 829, 964, 1617, 1746
TseFI	GTSAC	2 cut(s)	634, 2127
TseI	GCWGC	4 cut(s)	499, 1109, 1298, 1820
Tsp45I	GTSAC	2 cut(s)	634, 2127
TspDTI	ATGAA	7 cut(s)	256, 573, 1160, 1251, 1482, 1704, 1706
TspGWI	ACGGA	1 cut(s)	622
TspRI	CASTG	5 cut(s)	622, 829, 964, 1617, 1746
Vha464I	CTTAAG	1 cut(s)	494
VneI	GTGCAC	3 cut(s)	1317, 1440, 1608
VpaK11BI	GGWCC	4 cut(s)	164, 874, 1628, 2077
XagI	CCTNNNNNAGG	1 cut(s)	314
XapI	RAATTY	5 cut(s)	905, 1155, 1422, 1711, 1841
XceI	RCATGY	4 cut(s)	841, 972, 1052, 2045
XcmI	CCANNNNNNNNNTGG	1 cut(s)	348
XmaJI	CCTAGG	1 cut(s)	2116
XspI	CTAG	4 cut(s)	203, 1577, 2024, 2117
ZrmI	AGTACT	1 cut(s)	721
Zsp2I	ATGCAT	2 cut(s)	487, 1050

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.